AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043431.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043431.01.T01 MTR_8g035610 99.531 213 1 0 1 213 1 213 1.59e-160 442
MsG0880043431.01.T01 MTR_8g035610 99.497 199 1 0 15 213 1 199 1.46e-149 414
MsG0880043431.01.T01 MTR_7g075760 57.653 196 80 2 9 203 5 198 1.16e-80 239
MsG0880043431.01.T01 MTR_7g076050 57.653 196 80 2 9 203 5 198 1.16e-80 239
MsG0880043431.01.T01 MTR_7g076070 56.938 209 77 3 1 205 1 200 5.68e-78 233
MsG0880043431.01.T01 MTR_7g075710 57.971 207 74 4 2 204 1 198 1.21e-76 229
MsG0880043431.01.T01 MTR_7g075750 57.812 192 76 2 15 205 1 188 4.83e-73 220
MsG0880043431.01.T01 MTR_7g076045 59.231 130 47 2 76 205 5 128 9.58e-47 151
MsG0880043431.01.T01 MTR_7g074480 60.784 51 15 2 110 160 3 48 3.12e-12 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043431.01.T01 AT3G18030 78.894 199 41 1 13 211 9 206 5.86e-124 349
MsG0880043431.01.T01 AT1G48605 76.923 195 44 1 14 208 2 195 6.40e-118 333

Find 79 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTACAACAACTTTGATCTT+AGG 0.238461 8:-24730441 MsG0880043431.01.T01:three_prime_UTR
CCAACTGAACTCACAGGTTC+TGG 0.249432 8:+24732405 None:intergenic
AGGTTAGCTTGTGGGGATTA+TGG 0.262541 8:-24730668 MsG0880043431.01.T01:CDS
GAGCCTTACAGTAGAGTAAA+TGG 0.285370 8:+24730619 None:intergenic
GAGTAAAATCCGAGGCTTCC+TGG 0.300240 8:+24732353 None:intergenic
GTTCCACATAAAAGTGTTCA+TGG 0.333551 8:+24730763 None:intergenic
CTCACCCTCTGAGACATAAA+AGG 0.344105 8:+24730382 None:intergenic
GGTTTGTATACACACCCTTT+TGG 0.357670 8:-24730360 MsG0880043431.01.T01:three_prime_UTR
AAGATCTTTGTTGAAATCTC+TGG 0.366216 8:-24732443 MsG0880043431.01.T01:five_prime_UTR
TTTGTCATTGTTTCACTGAA+TGG 0.370610 8:-24732290 MsG0880043431.01.T01:CDS
TGTTTGCTGTTGTAGATTGC+TGG 0.375544 8:-24730860 MsG0880043431.01.T01:intron
TCACATGTATCGTGCGAGCC+TGG 0.385273 8:-24730817 MsG0880043431.01.T01:CDS
CAAGCTAACCTCTTTGTAAC+AGG 0.386122 8:+24730680 None:intergenic
GTGGTGTGTAATAGTGTAAA+TGG 0.407317 8:-24730419 MsG0880043431.01.T01:three_prime_UTR
AAAATAGCATCGACTTATCA+AGG 0.416965 8:+24730502 None:intergenic
AGTAAAATCCGAGGCTTCCT+GGG 0.419242 8:+24732354 None:intergenic
TTGTCATTGTTTCACTGAAT+GGG 0.424119 8:-24732289 MsG0880043431.01.T01:CDS
TTCACATTGAGCTTCGCAAA+TGG 0.429618 8:-24732137 MsG0880043431.01.T01:CDS
TACACGACCAAATATCCAAA+AGG 0.439002 8:+24730345 None:intergenic
TAAATATCCACCATTCAAAC+AGG 0.444612 8:+24732465 None:intergenic
CGTATACAAAATCACATCCT+TGG 0.455438 8:+24732206 None:intergenic
TGTATACGGACGACGATGAA+TGG 0.456117 8:-24732191 MsG0880043431.01.T01:CDS
CAACTGAACTCACAGGTTCT+GGG 0.458227 8:+24732406 None:intergenic
TTGTTTCCTTGTTTGTAGAA+TGG 0.460477 8:-24730287 MsG0880043431.01.T01:three_prime_UTR
CCATTGTCGGCAAACACTCT+TGG 0.468148 8:-24732093 MsG0880043431.01.T01:CDS
TTACAAAGAGGTTAGCTTGT+GGG 0.472912 8:-24730676 MsG0880043431.01.T01:CDS
TCAAGTATGAATGGTTTACT+AGG 0.475295 8:-24730538 MsG0880043431.01.T01:three_prime_UTR
CTACCATTTACTCTACTGTA+AGG 0.476278 8:-24730622 MsG0880043431.01.T01:CDS
AACCACTGTTCAAGTATGAA+TGG 0.476353 8:-24730547 MsG0880043431.01.T01:three_prime_UTR
TCACATTGAGCTTCGCAAAT+GGG 0.477326 8:-24732136 MsG0880043431.01.T01:CDS
CACATGTATCGTGCGAGCCT+GGG 0.485764 8:-24730816 MsG0880043431.01.T01:CDS
CTCATCTCCATTGACGAGCT+TGG 0.486057 8:-24730719 MsG0880043431.01.T01:CDS
ACAGTAGAGTAAATGGTAGA+AGG 0.491124 8:+24730626 None:intergenic
ACTTGAAACTCCTGTTTGAA+TGG 0.492773 8:-24732475 MsG0880043431.01.T01:five_prime_UTR
ATGGCTGGTGCAACAAAGAA+TGG 0.494922 8:+24730782 None:intergenic
AGAAATACCAAGCTCGTCAA+TGG 0.495582 8:+24730712 None:intergenic
TCGCAAATGGGCTGATATCA+TGG 0.497396 8:-24732124 MsG0880043431.01.T01:CDS
AAACTGGTTGAAGTAATTTG+AGG 0.501499 8:+24730264 None:intergenic
CCAAGAGTGTTTGCCGACAA+TGG 0.502597 8:+24732093 None:intergenic
CATGGTCATTGCTCCATTGT+CGG 0.504477 8:-24732106 MsG0880043431.01.T01:CDS
TTGAAATCTCTGGTTGATGA+TGG 0.505971 8:-24732433 MsG0880043431.01.T01:exon
GTATACAAAATCACATCCTT+GGG 0.508904 8:+24732207 None:intergenic
CACATAAAAGTGTTCATGGC+TGG 0.518632 8:+24730767 None:intergenic
TACAAAGAGGTTAGCTTGTG+GGG 0.520289 8:-24730675 MsG0880043431.01.T01:CDS
CCAGAACCTGTGAGTTCAGT+TGG 0.528188 8:-24732405 MsG0880043431.01.T01:CDS
ATGAGATGCCGCTCTGTGAA+AGG 0.531219 8:+24730737 None:intergenic
ACACGACCAAATATCCAAAA+GGG 0.534142 8:+24730346 None:intergenic
GAATGGCTTGCTATAGTCCC+AGG 0.538623 8:+24730799 None:intergenic
TGCTGTTGTAGATTGCTGGA+GGG 0.541864 8:-24730856 MsG0880043431.01.T01:intron
ACGACGATGAATGGTCTAGT+TGG 0.542274 8:-24732182 MsG0880043431.01.T01:CDS
GCTTGTGGGGATTATGGCAA+TGG 0.544428 8:-24730662 MsG0880043431.01.T01:CDS
ACAACAACTTTGATCTTAGG+TGG 0.545975 8:-24730438 MsG0880043431.01.T01:three_prime_UTR
GGATTATGGCAATGGTGCAA+TGG 0.551856 8:-24730654 MsG0880043431.01.T01:CDS
CAAAACACGACACACGAGAA+GGG 0.559732 8:-24733755 MsG0880043431.01.T01:five_prime_UTR
GACACACGAGAAGGGAAACA+AGG 0.561249 8:-24733747 MsG0880043431.01.T01:five_prime_UTR
AAATAGCATCGACTTATCAA+GGG 0.569320 8:+24730503 None:intergenic
TGAACTCACAGGTTCTGGGC+TGG 0.571240 8:+24732410 None:intergenic
TCAAAGGCTCAGCAAGGTAA+CGG 0.571549 8:-24730587 MsG0880043431.01.T01:CDS
TGGTCTAGTTGGAAGAAACT+AGG 0.573969 8:-24732171 MsG0880043431.01.T01:CDS
TCATTCCACCTGTTACAAAG+AGG 0.579111 8:-24730688 MsG0880043431.01.T01:CDS
TGAAACTCCTGTTTGAATGG+TGG 0.582594 8:-24732472 MsG0880043431.01.T01:five_prime_UTR
GCTAACCTCTTTGTAACAGG+TGG 0.589007 8:+24730683 None:intergenic
TTGAGGCCATTCTACAAACA+AGG 0.593358 8:+24730281 None:intergenic
ACAAAACACGACACACGAGA+AGG 0.595122 8:-24733756 MsG0880043431.01.T01:five_prime_UTR
TTCTCTCCAACTGAACTCAC+AGG 0.597337 8:+24732399 None:intergenic
AACCATTCATACTTGAACAG+TGG 0.599364 8:+24730545 None:intergenic
GTCGGCAAACACTCTTGGCA+AGG 0.605734 8:-24732088 MsG0880043431.01.T01:intron
TTGCTGTTGTAGATTGCTGG+AGG 0.607972 8:-24730857 MsG0880043431.01.T01:intron
TGACCTCAAACAAAAGCGAA+AGG 0.615486 8:+24733704 None:intergenic
TGAAAGAACAGCGATTCCCA+AGG 0.623450 8:-24732223 MsG0880043431.01.T01:CDS
TATGAGTCAAAGGCTCAGCA+AGG 0.625267 8:-24730593 MsG0880043431.01.T01:CDS
TACTAGTGGAAGTGTTGCTG+CGG 0.634094 8:-24732328 MsG0880043431.01.T01:CDS
GTTACAAAGAGGTTAGCTTG+TGG 0.645490 8:-24730677 MsG0880043431.01.T01:CDS
GTAAAATCCGAGGCTTCCTG+GGG 0.650776 8:+24732355 None:intergenic
AATAGCATCGACTTATCAAG+GGG 0.669762 8:+24730504 None:intergenic
ACACACGAGAAGGGAAACAA+GGG 0.700242 8:-24733746 MsG0880043431.01.T01:five_prime_UTR
CTAGTAGCGAGTAAAATCCG+AGG 0.704126 8:+24732345 None:intergenic
GGAACAATCCTTTCACAGAG+CGG 0.724970 8:-24730745 MsG0880043431.01.T01:CDS
TAAAATCCGAGGCTTCCTGG+GGG 0.763745 8:+24732356 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTATATTTTAATAACCA+AGG - Chr8:24731473-24731492 MsG0880043431.01.T01:intron 10.0%
!! TATATTCTATCTACTTATAA+AGG - Chr8:24732034-24732053 MsG0880043431.01.T01:intron 15.0%
!! TTAGAAATTGTAAAAGAAAA+TGG + Chr8:24732139-24732158 None:intergenic 15.0%
!!! AATGTTTTTAGAAAGTTAAT+AGG - Chr8:24732342-24732361 MsG0880043431.01.T01:CDS 15.0%
!!! TGATTTTCTGAAATTATTAT+AGG - Chr8:24731334-24731353 MsG0880043431.01.T01:intron 15.0%
!! ACAGTATAGAATATACATTT+AGG - Chr8:24731411-24731430 MsG0880043431.01.T01:intron 20.0%
!! AGTTTATATTTCAGTTTAAC+AGG - Chr8:24731095-24731114 MsG0880043431.01.T01:intron 20.0%
!! ATGTTAGGATTATAGAATAA+AGG + Chr8:24733083-24733102 None:intergenic 20.0%
!! TATAGAATATACATTTAGGT+TGG - Chr8:24731415-24731434 MsG0880043431.01.T01:intron 20.0%
!! TGTTAGGATTATAGAATAAA+GGG + Chr8:24733082-24733101 None:intergenic 20.0%
!! TGTTAGTTAGATTTCTATTT+GGG + Chr8:24732678-24732697 None:intergenic 20.0%
!! TTCAGTTTAAATTACTAACA+AGG + Chr8:24732296-24732315 None:intergenic 20.0%
!! TTGAATATAGATGAATCTAA+TGG + Chr8:24731453-24731472 None:intergenic 20.0%
!! TTGTTAGTTAGATTTCTATT+TGG + Chr8:24732679-24732698 None:intergenic 20.0%
!!! ATAGAATATACATTTAGGTT+GGG - Chr8:24731416-24731435 MsG0880043431.01.T01:intron 20.0%
!!! GGAAAATGTATTTTGATTTT+AGG - Chr8:24730505-24730524 MsG0880043431.01.T01:three_prime_UTR 20.0%
!!! TTAACAATGCTTGTATATTT+TGG - Chr8:24731030-24731049 MsG0880043431.01.T01:intron 20.0%
! ACCTTCTATTACAAAAGAAA+GGG - Chr8:24732594-24732613 MsG0880043431.01.T01:intron 25.0%
! ATTACAAAAGAAAGGGATAA+TGG - Chr8:24732601-24732620 MsG0880043431.01.T01:intron 25.0%
! ATTACAACAACTTTGATCTT+AGG - Chr8:24733626-24733645 MsG0880043431.01.T01:intron 25.0%
! CAAAAAACAAACCTTCAAAT+TGG + Chr8:24731568-24731587 None:intergenic 25.0%
! CTTTACATATTACTACTACA+TGG + Chr8:24731191-24731210 None:intergenic 25.0%
! GCATATATCTTAGTTGTAAT+TGG + Chr8:24732535-24732554 None:intergenic 25.0%
! GTTGAATTGAAAATGAAAAC+AGG + Chr8:24732079-24732098 None:intergenic 25.0%
! TCCATTTAAAAAAGATTCCT+TGG + Chr8:24731493-24731512 None:intergenic 25.0%
! TCTATAATCCTAACATATGA+TGG - Chr8:24733087-24733106 MsG0880043431.01.T01:intron 25.0%
! TTACAAAAGAAAGGGATAAT+GGG - Chr8:24732602-24732621 MsG0880043431.01.T01:intron 25.0%
! TTCTATTACATTACACACTA+TGG + Chr8:24730743-24730762 None:intergenic 25.0%
!! ATTTTACGTTACCAATTTGA+AGG - Chr8:24731554-24731573 MsG0880043431.01.T01:intron 25.0%
!! TCTGAAATTATTATAGGTGT+CGG - Chr8:24731340-24731359 MsG0880043431.01.T01:intron 25.0%
!! TTAGAAGTTGGTTTATGATT+TGG - Chr8:24731157-24731176 MsG0880043431.01.T01:intron 25.0%
!!! ACCAAGGAATCTTTTTTAAA+TGG - Chr8:24731489-24731508 MsG0880043431.01.T01:intron 25.0%
AAGATCTTTGTTGAAATCTC+TGG - Chr8:24731624-24731643 MsG0880043431.01.T01:intron 30.0%
AGTGTGTAATGTAATAGAAC+AGG - Chr8:24730744-24730763 MsG0880043431.01.T01:CDS 30.0%
ATTATGTACTGTCCTCTAAA+GGG - Chr8:24731531-24731550 MsG0880043431.01.T01:intron 30.0%
CAATTGTGATAGTAAGAAGA+TGG + Chr8:24732250-24732269 None:intergenic 30.0%
GAAAATGAAAACAGGAAACA+TGG + Chr8:24732071-24732090 None:intergenic 30.0%
GACCTTCTATTACAAAAGAA+AGG - Chr8:24732593-24732612 MsG0880043431.01.T01:intron 30.0%
GGGAAGAACAATGTATTAAT+GGG - Chr8:24730650-24730669 MsG0880043431.01.T01:CDS 30.0%
GTATACAAAATCACATCCTT+GGG + Chr8:24731863-24731882 None:intergenic 30.0%
GTGTATGTACATCTTATGTA+TGG - Chr8:24733116-24733135 MsG0880043431.01.T01:intron 30.0%
TAAATATCCACCATTCAAAC+AGG + Chr8:24731605-24731624 None:intergenic 30.0%
TACACAACATGTGAAGAATT+CGG + Chr8:24732943-24732962 None:intergenic 30.0%
TCAAGTATGAATGGTTTACT+AGG - Chr8:24733529-24733548 MsG0880043431.01.T01:intron 30.0%
TCTAGAAACAATACAAACAG+TGG + Chr8:24732203-24732222 None:intergenic 30.0%
TTACTATGAGTTAGTTAGCT+AGG + Chr8:24732709-24732728 None:intergenic 30.0%
TTGTCATTGTTTCACTGAAT+GGG - Chr8:24731778-24731797 MsG0880043431.01.T01:intron 30.0%
TTTGTCATTGTTTCACTGAA+TGG - Chr8:24731777-24731796 MsG0880043431.01.T01:intron 30.0%
! AAAATGCAAAGACGATTTTG+TGG + Chr8:24731822-24731841 None:intergenic 30.0%
! AACTCATGTCATTTAGAAGT+TGG - Chr8:24731145-24731164 MsG0880043431.01.T01:intron 30.0%
! ATGCCAAAGATACTTTTCTA+AGG - Chr8:24732551-24732570 MsG0880043431.01.T01:intron 30.0%
! CAAGGATGTGATTTTGTATA+CGG - Chr8:24731862-24731881 MsG0880043431.01.T01:intron 30.0%
! TACCATCATTGAAAAAGTGT+AGG + Chr8:24730477-24730496 None:intergenic 30.0%
! TCCCTTTCTTTTGTAATAGA+AGG + Chr8:24732598-24732617 None:intergenic 30.0%
! TGCCAAAGATACTTTTCTAA+GGG - Chr8:24732552-24732571 MsG0880043431.01.T01:intron 30.0%
! TTGTTTCCTTGTTTGTAGAA+TGG - Chr8:24733780-24733799 MsG0880043431.01.T01:five_prime_UTR 30.0%
!! AAAATAGCATCGACTTATCA+AGG + Chr8:24733568-24733587 None:intergenic 30.0%
!! AAATAGCATCGACTTATCAA+GGG + Chr8:24733567-24733586 None:intergenic 30.0%
!! TTCCCTTAGAAAAGTATCTT+TGG + Chr8:24732557-24732576 None:intergenic 30.0%
!! TTTTGTTGTATTTCCGTTAC+AGG + Chr8:24732648-24732667 None:intergenic 30.0%
!!! ACTTTTTCAATGATGGTAGA+GGG - Chr8:24730479-24730498 MsG0880043431.01.T01:three_prime_UTR 30.0%
!!! TAGTCTGTTTTGATTCGATA+AGG + Chr8:24730796-24730815 None:intergenic 30.0%
!!! TTCCTACACTTTTTCAATGA+TGG - Chr8:24730472-24730491 MsG0880043431.01.T01:three_prime_UTR 30.0%
AAAGAAAGGATAACGCTGAT+TGG - Chr8:24732860-24732879 MsG0880043431.01.T01:intron 35.0%
AACCACTGTTCAAGTATGAA+TGG - Chr8:24733520-24733539 MsG0880043431.01.T01:intron 35.0%
AACCATTCATACTTGAACAG+TGG + Chr8:24733525-24733544 None:intergenic 35.0%
AATCAGCGTTATCCTTTCTT+TGG + Chr8:24732861-24732880 None:intergenic 35.0%
ACAACAACTTTGATCTTAGG+TGG - Chr8:24733629-24733648 MsG0880043431.01.T01:intron 35.0%
ACAACTGACCATCATATGTT+AGG + Chr8:24733098-24733117 None:intergenic 35.0%
ACACGACCAAATATCCAAAA+GGG + Chr8:24733724-24733743 None:intergenic 35.0%
ACACGCAAATCATCAAAAAG+AGG + Chr8:24730588-24730607 None:intergenic 35.0%
ACAGTAGAGTAAATGGTAGA+AGG + Chr8:24733444-24733463 None:intergenic 35.0%
ACATAATTCGCAATTCGCAT+TGG + Chr8:24732986-24733005 None:intergenic 35.0%
ACTTGAAACTCCTGTTTGAA+TGG - Chr8:24731592-24731611 MsG0880043431.01.T01:intron 35.0%
AGTAACATAATTCGTGCACT+AGG - Chr8:24732724-24732743 MsG0880043431.01.T01:intron 35.0%
AGTTTAACAGGACAAAGAGA+TGG - Chr8:24731107-24731126 MsG0880043431.01.T01:intron 35.0%
ATAGTAAGAAGATGGAAGAC+TGG + Chr8:24732242-24732261 None:intergenic 35.0%
CACGCAAATCATCAAAAAGA+GGG + Chr8:24730587-24730606 None:intergenic 35.0%
CATTATGTACTGTCCTCTAA+AGG - Chr8:24731530-24731549 MsG0880043431.01.T01:intron 35.0%
CGTATACAAAATCACATCCT+TGG + Chr8:24731864-24731883 None:intergenic 35.0%
CTACCATTTACTCTACTGTA+AGG - Chr8:24733445-24733464 MsG0880043431.01.T01:intron 35.0%
CTTATTCCATTAAGTGGGAT+CGG - Chr8:24730698-24730717 MsG0880043431.01.T01:CDS 35.0%
GCGTTGCTAACATCATATAA+CGG + Chr8:24732427-24732446 None:intergenic 35.0%
GGGGAAGAACAATGTATTAA+TGG - Chr8:24730649-24730668 MsG0880043431.01.T01:CDS 35.0%
GTAACGTAAAATCCCTTTAG+AGG + Chr8:24731546-24731565 None:intergenic 35.0%
GTACAGTATACTATGAAGCA+TGG - Chr8:24731254-24731273 MsG0880043431.01.T01:intron 35.0%
GTGGTGTGTAATAGTGTAAA+TGG - Chr8:24733648-24733667 MsG0880043431.01.T01:intron 35.0%
TACACGACCAAATATCCAAA+AGG + Chr8:24733725-24733744 None:intergenic 35.0%
TCGTGTGATTAATCGTTAGT+CGG - Chr8:24730627-24730646 MsG0880043431.01.T01:CDS 35.0%
TCTATACTGTCTATGAAGCA+CGG + Chr8:24731401-24731420 None:intergenic 35.0%
! AAGGCTCTTTTATGAGTCAA+AGG - Chr8:24733464-24733483 MsG0880043431.01.T01:intron 35.0%
! ACGCTGATTGGTGAAAAAAA+CGG - Chr8:24732872-24732891 MsG0880043431.01.T01:intron 35.0%
! ATTCTCCTTTTATGTCTCAG+AGG - Chr8:24733680-24733699 MsG0880043431.01.T01:intron 35.0%
! CGAGATAAAGTCTCTAAAAG+AGG + Chr8:24731067-24731086 None:intergenic 35.0%
! TACACACCCTTTTGGATATT+TGG - Chr8:24733715-24733734 MsG0880043431.01.T01:five_prime_UTR 35.0%
! TTCTCCTTTTATGTCTCAGA+GGG - Chr8:24733681-24733700 MsG0880043431.01.T01:intron 35.0%
!! AAAGAAAGGGATAATGGGTA+AGG - Chr8:24732607-24732626 MsG0880043431.01.T01:intron 35.0%
!! AATAGCATCGACTTATCAAG+GGG + Chr8:24733566-24733585 None:intergenic 35.0%
!! CAAGCCTTATTCCATTAAGT+GGG - Chr8:24730693-24730712 MsG0880043431.01.T01:CDS 35.0%
!! GATACTTTTCTAAGGGAAGA+AGG - Chr8:24732559-24732578 MsG0880043431.01.T01:intron 35.0%
!! GCTAACATCATATAACGGAT+AGG + Chr8:24732422-24732441 None:intergenic 35.0%
!! GTTCCACATAAAAGTGTTCA+TGG + Chr8:24733307-24733326 None:intergenic 35.0%
!! TTACAAAGAGGTTAGCTTGT+GGG - Chr8:24733391-24733410 MsG0880043431.01.T01:intron 35.0%
!! TTGAAATCTCTGGTTGATGA+TGG - Chr8:24731634-24731653 MsG0880043431.01.T01:intron 35.0%
!!! CACTTTTTCAATGATGGTAG+AGG - Chr8:24730478-24730497 MsG0880043431.01.T01:three_prime_UTR 35.0%
ACAGGAAACATGGAAGCAAA+CGG + Chr8:24732061-24732080 None:intergenic 40.0%
ACATCGTCACACAAACTTGT+CGG + Chr8:24732396-24732415 None:intergenic 40.0%
AGAAATACCAAGCTCGTCAA+TGG + Chr8:24733358-24733377 None:intergenic 40.0%
ATTCCTGTTTACACTGCAGT+AGG - Chr8:24730939-24730958 MsG0880043431.01.T01:intron 40.0%
CAAGCTAACCTCTTTGTAAC+AGG + Chr8:24733390-24733409 None:intergenic 40.0%
CATATTTGTCAGTGTCGTGT+CGG + Chr8:24731298-24731317 None:intergenic 40.0%
CCACTTAATGGAATAAGGCT+TGG + Chr8:24730695-24730714 None:intergenic 40.0%
CCTTAGCAACTAACCTGTAA+CGG - Chr8:24732632-24732651 MsG0880043431.01.T01:intron 40.0%
CGATCCCACTTAATGGAATA+AGG + Chr8:24730700-24730719 None:intergenic 40.0%
CGTGTGATTAATCGTTAGTC+GGG - Chr8:24730628-24730647 MsG0880043431.01.T01:CDS 40.0%
CTTGTCGGAACTAAAGCAAT+TGG + Chr8:24732381-24732400 None:intergenic 40.0%
GAGCCTTACAGTAGAGTAAA+TGG + Chr8:24733451-24733470 None:intergenic 40.0%
GAGTTAGTTAGCTAGGTTCA+AGG + Chr8:24732702-24732721 None:intergenic 40.0%
GTGTGATTAATCGTTAGTCG+GGG - Chr8:24730629-24730648 MsG0880043431.01.T01:CDS 40.0%
TAACAGGACAAAGAGATGGT+AGG - Chr8:24731111-24731130 MsG0880043431.01.T01:intron 40.0%
TATGTAGCAGTACCCGAATT+CGG + Chr8:24732786-24732805 None:intergenic 40.0%
TCACATTGAGCTTCGCAAAT+GGG - Chr8:24731931-24731950 MsG0880043431.01.T01:intron 40.0%
TCATTCCACCTGTTACAAAG+AGG - Chr8:24733379-24733398 MsG0880043431.01.T01:intron 40.0%
TGAAACTCCTGTTTGAATGG+TGG - Chr8:24731595-24731614 MsG0880043431.01.T01:intron 40.0%
TGACCTCAAACAAAAGCGAA+AGG + Chr8:24730366-24730385 None:intergenic 40.0%
TGGTCTAGTTGGAAGAAACT+AGG - Chr8:24731896-24731915 MsG0880043431.01.T01:intron 40.0%
TGTGATTAATCGTTAGTCGG+GGG - Chr8:24730630-24730649 MsG0880043431.01.T01:CDS 40.0%
TTCAATGATGGTAGAGGGTT+AGG - Chr8:24730484-24730503 MsG0880043431.01.T01:three_prime_UTR 40.0%
TTCACATTGAGCTTCGCAAA+TGG - Chr8:24731930-24731949 MsG0880043431.01.T01:intron 40.0%
TTGAGATGCATCAATTCACG+TGG - Chr8:24732806-24732825 MsG0880043431.01.T01:intron 40.0%
TTGAGGCCATTCTACAAACA+AGG + Chr8:24733789-24733808 None:intergenic 40.0%
TTTCCTACTGCAGTGTAAAC+AGG + Chr8:24730945-24730964 None:intergenic 40.0%
! CAGCCATGAACACTTTTATG+TGG - Chr8:24733301-24733320 MsG0880043431.01.T01:intron 40.0%
! GGTTTGTATACACACCCTTT+TGG - Chr8:24733707-24733726 MsG0880043431.01.T01:intron 40.0%
!! ACTCCTTTCGCTTTTGTTTG+AGG - Chr8:24730360-24730379 MsG0880043431.01.T01:three_prime_UTR 40.0%
!! CACATAAAAGTGTTCATGGC+TGG + Chr8:24733303-24733322 None:intergenic 40.0%
!! CCAAGCCTTATTCCATTAAG+TGG - Chr8:24730692-24730711 MsG0880043431.01.T01:CDS 40.0%
!! GTTACAAAGAGGTTAGCTTG+TGG - Chr8:24733390-24733409 MsG0880043431.01.T01:intron 40.0%
!! TACAAAGAGGTTAGCTTGTG+GGG - Chr8:24733392-24733411 MsG0880043431.01.T01:intron 40.0%
!! TGTTTGCTGTTGTAGATTGC+TGG - Chr8:24733207-24733226 MsG0880043431.01.T01:intron 40.0%
AAAGAGATGGTAGGTGGCTT+TGG - Chr8:24731120-24731139 MsG0880043431.01.T01:intron 45.0%
ACAAAACACGACACACGAGA+AGG - Chr8:24730311-24730330 MsG0880043431.01.T01:three_prime_UTR 45.0%
ACACACGAGAAGGGAAACAA+GGG - Chr8:24730321-24730340 MsG0880043431.01.T01:three_prime_UTR 45.0%
ACGACGATGAATGGTCTAGT+TGG - Chr8:24731885-24731904 MsG0880043431.01.T01:intron 45.0%
AGTAAAATCCGAGGCTTCCT+GGG + Chr8:24731716-24731735 None:intergenic 45.0%
ATACCAGGCGTACCAAAGAA+AGG - Chr8:24732846-24732865 MsG0880043431.01.T01:intron 45.0%
ATGGCTGGTGCAACAAAGAA+TGG + Chr8:24733288-24733307 None:intergenic 45.0%
ATGTAGCCGATCCCACTTAA+TGG + Chr8:24730707-24730726 None:intergenic 45.0%
CAAAACACGACACACGAGAA+GGG - Chr8:24730312-24730331 MsG0880043431.01.T01:three_prime_UTR 45.0%
CAACTGAACTCACAGGTTCT+GGG + Chr8:24731664-24731683 None:intergenic 45.0%
CAGGAAACATGGAAGCAAAC+GGG + Chr8:24732060-24732079 None:intergenic 45.0%
CATGGTCATTGCTCCATTGT+CGG - Chr8:24731961-24731980 MsG0880043431.01.T01:intron 45.0%
CTAGTAGCGAGTAAAATCCG+AGG + Chr8:24731725-24731744 None:intergenic 45.0%
GATGTTAGCAACGCAAGAGT+TGG - Chr8:24732433-24732452 MsG0880043431.01.T01:exon 45.0%
GCACAAATCTCACTGTCGAT+AGG + Chr8:24730828-24730847 None:intergenic 45.0%
GCTAACCTCTTTGTAACAGG+TGG + Chr8:24733387-24733406 None:intergenic 45.0%
GGAACAATCCTTTCACAGAG+CGG - Chr8:24733322-24733341 MsG0880043431.01.T01:intron 45.0%
GGATTATGGCAATGGTGCAA+TGG - Chr8:24733413-24733432 MsG0880043431.01.T01:intron 45.0%
TATCCTTTCTTTGGTACGCC+TGG + Chr8:24732852-24732871 None:intergenic 45.0%
TATGAGTCAAAGGCTCAGCA+AGG - Chr8:24733474-24733493 MsG0880043431.01.T01:intron 45.0%
TCAAAGGCTCAGCAAGGTAA+CGG - Chr8:24733480-24733499 MsG0880043431.01.T01:intron 45.0%
TGAAAGAACAGCGATTCCCA+AGG - Chr8:24731844-24731863 MsG0880043431.01.T01:intron 45.0%
TGCTGTTGTAGATTGCTGGA+GGG - Chr8:24733211-24733230 MsG0880043431.01.T01:intron 45.0%
TGTATACGGACGACGATGAA+TGG - Chr8:24731876-24731895 MsG0880043431.01.T01:intron 45.0%
TTCTCTCCAACTGAACTCAC+AGG + Chr8:24731671-24731690 None:intergenic 45.0%
TTTCTTTGGTACGCCTGGTA+TGG + Chr8:24732847-24732866 None:intergenic 45.0%
TTTGCACAAACTGCCATACC+AGG - Chr8:24732831-24732850 MsG0880043431.01.T01:intron 45.0%
! AAGAAGATGGAAGACTGGCT+AGG + Chr8:24732237-24732256 None:intergenic 45.0%
! AAGAGAGTGTGAGTCCTACT+AGG - Chr8:24730886-24730905 MsG0880043431.01.T01:intron 45.0%
! AGAGAGTGTGAGTCCTACTA+GGG - Chr8:24730887-24730906 MsG0880043431.01.T01:intron 45.0%
! CCGTTACAGGTTAGTTGCTA+AGG + Chr8:24732635-24732654 None:intergenic 45.0%
! CGGATTTTACTCGCTACTAG+TGG - Chr8:24731725-24731744 MsG0880043431.01.T01:intron 45.0%
! CTTTTATGTCTCAGAGGGTG+AGG - Chr8:24733686-24733705 MsG0880043431.01.T01:intron 45.0%
! TCGCAATTCGCATTGGTACA+CGG + Chr8:24732979-24732998 None:intergenic 45.0%
! TTGCTGTTGTAGATTGCTGG+AGG - Chr8:24733210-24733229 MsG0880043431.01.T01:intron 45.0%
!! AATTCGCGAGTACCGAATTC+GGG - Chr8:24732771-24732790 MsG0880043431.01.T01:intron 45.0%
!! AGAACAGGTAAAAAGCGTCG+AGG - Chr8:24730759-24730778 MsG0880043431.01.T01:CDS 45.0%
!! AGGTTAGCTTGTGGGGATTA+TGG - Chr8:24733399-24733418 MsG0880043431.01.T01:intron 45.0%
!! CAATTCGCGAGTACCGAATT+CGG - Chr8:24732770-24732789 MsG0880043431.01.T01:intron 45.0%
!! CTCACCCTCTGAGACATAAA+AGG + Chr8:24733688-24733707 None:intergenic 45.0%
!! TACTAGTGGAAGTGTTGCTG+CGG - Chr8:24731739-24731758 MsG0880043431.01.T01:intron 45.0%
!! TCGCAAATGGGCTGATATCA+TGG - Chr8:24731943-24731962 MsG0880043431.01.T01:intron 45.0%
ACGGATACAGACACGTACAC+CGG + Chr8:24731382-24731401 None:intergenic 50.0%
ATGAGATGCCGCTCTGTGAA+AGG + Chr8:24733333-24733352 None:intergenic 50.0%
CAGGACAAAGAGATGGTAGG+TGG - Chr8:24731114-24731133 MsG0880043431.01.T01:intron 50.0%
CCAACTGAACTCACAGGTTC+TGG + Chr8:24731665-24731684 None:intergenic 50.0%
CCAGAACCTGTGAGTTCAGT+TGG - Chr8:24731662-24731681 MsG0880043431.01.T01:intron 50.0%
CCATTGTCGGCAAACACTCT+TGG - Chr8:24731974-24731993 MsG0880043431.01.T01:intron 50.0%
CGAATTCGGTACTCGCGAAT+TGG + Chr8:24732772-24732791 None:intergenic 50.0%
CTCATCTCCATTGACGAGCT+TGG - Chr8:24733348-24733367 MsG0880043431.01.T01:intron 50.0%
GACACACGAGAAGGGAAACA+AGG - Chr8:24730320-24730339 MsG0880043431.01.T01:three_prime_UTR 50.0%
GAGTAAAATCCGAGGCTTCC+TGG + Chr8:24731717-24731736 None:intergenic 50.0%
GCTTGTGGGGATTATGGCAA+TGG - Chr8:24733405-24733424 MsG0880043431.01.T01:intron 50.0%
GTAAAATCCGAGGCTTCCTG+GGG + Chr8:24731715-24731734 None:intergenic 50.0%
TAAAATCCGAGGCTTCCTGG+GGG + Chr8:24731714-24731733 None:intergenic 50.0%
! CGAATTGGTTCACGCCTTCA+CGG + Chr8:24732757-24732776 None:intergenic 50.0%
! GAGAGTGTGAGTCCTACTAG+GGG - Chr8:24730888-24730907 MsG0880043431.01.T01:intron 50.0%
! TTCGCATTGGTACACGGTAC+AGG + Chr8:24732973-24732992 None:intergenic 50.0%
!! CCAAGAGTGTTTGCCGACAA+TGG + Chr8:24731977-24731996 None:intergenic 50.0%
!! GAATGGCTTGCTATAGTCCC+AGG + Chr8:24733271-24733290 None:intergenic 50.0%
CACATGTATCGTGCGAGCCT+GGG - Chr8:24733251-24733270 MsG0880043431.01.T01:intron 55.0%
CACTAGGCAGAGAACCGTGA+AGG - Chr8:24732740-24732759 MsG0880043431.01.T01:intron 55.0%
CACTTGACGCATCCCCTAGT+AGG + Chr8:24730903-24730922 None:intergenic 55.0%
CTACTAGGGGATGCGTCAAG+TGG - Chr8:24730901-24730920 MsG0880043431.01.T01:intron 55.0%
GAGAGCAAGCTCTTCTGCAC+TGG - Chr8:24732501-24732520 MsG0880043431.01.T01:intron 55.0%
GTCGGCAAACACTCTTGGCA+AGG - Chr8:24731979-24731998 MsG0880043431.01.T01:intron 55.0%
TCACATGTATCGTGCGAGCC+TGG - Chr8:24733250-24733269 MsG0880043431.01.T01:intron 55.0%
TGGTACACGGTACAGGTTCG+CGG + Chr8:24732966-24732985 None:intergenic 55.0%
TGTCAGTGTCGTGTCGGAGT+TGG + Chr8:24731292-24731311 None:intergenic 55.0%
!! TGAACTCACAGGTTCTGGGC+TGG + Chr8:24731660-24731679 None:intergenic 55.0%
CGGCATGTCTGTGTCGTGTC+CGG - Chr8:24731360-24731379 MsG0880043431.01.T01:intron 60.0%
GTGTCGGAGTTGGACACGCT+CGG + Chr8:24731282-24731301 None:intergenic 60.0%
CAGTGAATGCTGCTGCCCCC+AGG - Chr8:24731696-24731715 MsG0880043431.01.T01:intron 65.0%
CTGCTGCCCCCAGGAAGCCT+CGG - Chr8:24731705-24731724 MsG0880043431.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr8 gene 24730275 24733814 24730275 ID=MsG0880043431.01;Name=MsG0880043431.01
Chr8 mRNA 24730275 24733814 24730275 ID=MsG0880043431.01.T01;Parent=MsG0880043431.01;Name=MsG0880043431.01.T01;_AED=0.49;_eAED=0.49;_QI=181|1|1|1|0.5|0.33|3|302|213
Chr8 exon 24730275 24730867 24730275 ID=MsG0880043431.01.T01:exon:9177;Parent=MsG0880043431.01.T01
Chr8 exon 24732089 24732513 24732089 ID=MsG0880043431.01.T01:exon:9176;Parent=MsG0880043431.01.T01
Chr8 exon 24733708 24733814 24733708 ID=MsG0880043431.01.T01:exon:9175;Parent=MsG0880043431.01.T01
Chr8 five_prime_UTR 24733708 24733814 24733708 ID=MsG0880043431.01.T01:five_prime_utr;Parent=MsG0880043431.01.T01
Chr8 five_prime_UTR 24732440 24732513 24732440 ID=MsG0880043431.01.T01:five_prime_utr;Parent=MsG0880043431.01.T01
Chr8 CDS 24732089 24732439 24732089 ID=MsG0880043431.01.T01:cds;Parent=MsG0880043431.01.T01
Chr8 CDS 24730577 24730867 24730577 ID=MsG0880043431.01.T01:cds;Parent=MsG0880043431.01.T01
Chr8 three_prime_UTR 24730275 24730576 24730275 ID=MsG0880043431.01.T01:three_prime_utr;Parent=MsG0880043431.01.T01
Gene Sequence

>MsG0880043431.01.T01

ATGATGGCCAGCCCAGAACCTGTGAGTTCAGTTGGAGAGAATATGTCAGTGAATGCTGCTGCCCCCAGGAAGCCTCGGATTTTACTCGCTACTAGTGGAAGTGTTGCTGCGGTTAAATTTGCAAATCTTTGTCATTGTTTCACTGAATGGGCAGAAGTAAGAGCTGTTGCCACAAAATCGTCTTTGCATTTTATTGAAAGAACAGCGATTCCCAAGGATGTGATTTTGTATACGGACGACGATGAATGGTCTAGTTGGAAGAAACTAGGCGATAGCGTGCTTCACATTGAGCTTCGCAAATGGGCTGATATCATGGTCATTGCTCCATTGTCGGCAAACACTCTTGGCAAGATTGCTGGAGGGTTGTGTGACAATCTACTCACATGTATCGTGCGAGCCTGGGACTATAGCAAGCCATTCTTTGTTGCACCAGCCATGAACACTTTTATGTGGAACAATCCTTTCACAGAGCGGCATCTCATCTCCATTGACGAGCTTGGTATTTCTCTCATTCCACCTGTTACAAAGAGGTTAGCTTGTGGGGATTATGGCAATGGTGCAATGGCTGAACCTTCTACCATTTACTCTACTGTAAGGCTCTTTTATGAGTCAAAGGCTCAGCAAGGTAACGGCGATGTTTAG
Protein sequence

>MsG0880043431.01.T01

MMASPEPVSSVGENMSVNAAAPRKPRILLATSGSVAAVKFANLCHCFTEWAEVRAVATKSSLHFIERTAIPKDVILYTDDDEWSSWKKLGDSVLHIELRKWADIMVIAPLSANTLGKIAGGLCDNLLTCIVRAWDYSKPFFVAPAMNTFMWNNPFTERHLISIDELGISLIPPVTKRLACGDYGNGAMAEPSTIYSTVRLFYESKAQQGNGDV*