AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580025385.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025385.01.T01 MTR_5g027460 72.519 131 34 2 31 159 412 542 1.17e-54 181
MsG0580025385.01.T01 MTR_5g027460 100.000 33 0 0 1 33 227 259 4.89e-13 66.2
MsG0580025385.01.T01 MTR_8g071050 42.222 135 66 4 27 159 376 500 2.98e-23 95.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 43 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAGCTCTCCGGTGGTTTC+TGG 0.133359 5:+17833140 MsG0580025385.01.T01:CDS
GGAATTGTTGTGTTGCTATT+TGG 0.217379 5:+17833060 MsG0580025385.01.T01:CDS
AAGAAGGTGCTTTCTCTATA+TGG 0.334773 5:+17832928 MsG0580025385.01.T01:CDS
TGCTTTCTTGAGAGTTGTTC+CGG 0.346386 5:-17832259 None:intergenic
ACGGACACAGTCACTGGAAA+TGG 0.347797 5:+17832814 MsG0580025385.01.T01:CDS
GCTGGTGGTGCTGTTGCTGC+AGG 0.363219 5:+17833095 MsG0580025385.01.T01:CDS
TCACTTCATGCTACTGCTGC+TGG 0.374544 5:+17832318 MsG0580025385.01.T01:CDS
CAGTTGGGCCAGGACGGATT+AGG 0.378121 5:+17833000 MsG0580025385.01.T01:CDS
TCAGGGTTTGGATTCTATTA+CGG 0.378827 5:+17832795 MsG0580025385.01.T01:intron
GAGCTGCAGGTTTGTGTGTC+GGG 0.405501 5:+17832964 MsG0580025385.01.T01:CDS
CGGACACAGTCACTGGAAAT+GGG 0.411485 5:+17832815 MsG0580025385.01.T01:CDS
CTTTCTCTATATGGTATGCT+TGG 0.415039 5:+17832937 MsG0580025385.01.T01:CDS
GGGTTCACAGTTGGTTCAGT+TGG 0.419215 5:+17832984 MsG0580025385.01.T01:CDS
CTTGAGAGTTGTTCCGGTGA+AGG 0.430405 5:-17832253 None:intergenic
CTCATGATCCGCGCCTTCAC+CGG 0.455419 5:+17832240 None:intergenic
GGTTCACAGTTGGTTCAGTT+GGG 0.464283 5:+17832985 MsG0580025385.01.T01:CDS
TCTATTACGGACACAGTCAC+TGG 0.495368 5:+17832808 MsG0580025385.01.T01:CDS
ATTTGGAAACTCAGTGATGC+TGG 0.502013 5:+17833077 MsG0580025385.01.T01:CDS
GGGTTAGATTTGTGCTCCAT+CGG 0.505069 5:+17832904 MsG0580025385.01.T01:CDS
CTGCCATTCAGAGCACATCC+AGG 0.506053 5:+17832855 MsG0580025385.01.T01:CDS
CCAAAAGCGTGCAAAATCCC+TGG 0.514306 5:-17832873 None:intergenic
ACAGTTGGTTCAGTTGGGCC+AGG 0.514316 5:+17832990 MsG0580025385.01.T01:CDS
AGTAGCATGAAGTGAGAGGC+GGG 0.517268 5:-17832310 None:intergenic
GGACGGATTAGGACTTCGTT+CGG 0.527260 5:+17833011 MsG0580025385.01.T01:CDS
TTGTGTGTCGGGTTCACAGT+TGG 0.527867 5:+17832975 MsG0580025385.01.T01:CDS
CTGGTGGTGCTGTTGCTGCA+GGG 0.528747 5:+17833096 MsG0580025385.01.T01:CDS
AGAGCTGCAGGTTTGTGTGT+CGG 0.529302 5:+17832963 MsG0580025385.01.T01:CDS
ATCCCTGGATGTGCTCTGAA+TGG 0.535476 5:-17832858 None:intergenic
TTTGTGCTCCATCGGAAAGA+AGG 0.540712 5:+17832912 MsG0580025385.01.T01:CDS
AGAAAGCACCTTCTTTCCGA+TGG 0.541888 5:-17832920 None:intergenic
CGATAATGAGAGAAGCTCTC+CGG 0.546258 5:+17833130 MsG0580025385.01.T01:CDS
AACGAAGTCCTAATCCGTCC+TGG 0.555456 5:-17833008 None:intergenic
ACAACAATTCCAGCAATAGC+AGG 0.576506 5:-17833048 None:intergenic
TATGCTTGGATGAGAGCTGC+AGG 0.590623 5:+17832951 MsG0580025385.01.T01:CDS
TTGGTTCAGTTGGGCCAGGA+CGG 0.593555 5:+17832994 MsG0580025385.01.T01:CDS
CAGCAGTAGCATGAAGTGAG+AGG 0.596792 5:-17832314 None:intergenic
TGCCATTCAGAGCACATCCA+GGG 0.607697 5:+17832856 MsG0580025385.01.T01:CDS
CAGTAGCATGAAGTGAGAGG+CGG 0.616592 5:-17832311 None:intergenic
GAATCTAAACCAGAAACCAC+CGG 0.628298 5:-17833149 None:intergenic
GAGTTGTTCCGGTGAAGGCG+CGG 0.634731 5:-17832248 None:intergenic
GGACACAGTCACTGGAAATG+GGG 0.658492 5:+17832816 MsG0580025385.01.T01:CDS
TAATGAGAGAAGCTCTCCGG+TGG 0.661539 5:+17833133 MsG0580025385.01.T01:CDS
TGGAAACTCAGTGATGCTGG+TGG 0.737554 5:+17833080 MsG0580025385.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GATATTTGAAGATGGAAAAA+GGG - Chr5:17832532-17832551 None:intergenic 25.0%
GGATATTTGAAGATGGAAAA+AGG - Chr5:17832533-17832552 None:intergenic 30.0%
TCGATTTCAGTGGTTTAATT+TGG + Chr5:17832434-17832453 MsG0580025385.01.T01:intron 30.0%
TTCAAATATCCTCATGCTAT+TGG + Chr5:17832542-17832561 MsG0580025385.01.T01:intron 30.0%
AAATATCCTCATGCTATTGG+AGG + Chr5:17832545-17832564 MsG0580025385.01.T01:intron 35.0%
AGAGCGATATTATGAGTCTT+TGG + Chr5:17832405-17832424 MsG0580025385.01.T01:intron 35.0%
AGCATGAGGATATTTGAAGA+TGG - Chr5:17832540-17832559 None:intergenic 35.0%
CAGAACAAAAGCAAATGCAA+TGG - Chr5:17832657-17832676 None:intergenic 35.0%
CTTTCTCTATATGGTATGCT+TGG + Chr5:17832937-17832956 MsG0580025385.01.T01:CDS 35.0%
GAGCGATATTATGAGTCTTT+GGG + Chr5:17832406-17832425 MsG0580025385.01.T01:intron 35.0%
TCAGGGTTTGGATTCTATTA+CGG + Chr5:17832795-17832814 MsG0580025385.01.T01:intron 35.0%
! GCACAGTTTTTCATTAGACA+AGG + Chr5:17832279-17832298 MsG0580025385.01.T01:CDS 35.0%
! GGAATTGTTGTGTTGCTATT+TGG + Chr5:17833060-17833079 MsG0580025385.01.T01:CDS 35.0%
!! AAGAAGGTGCTTTCTCTATA+TGG + Chr5:17832928-17832947 MsG0580025385.01.T01:CDS 35.0%
!! AGTGGTTTAATTTGGCTTGT+TGG + Chr5:17832442-17832461 MsG0580025385.01.T01:intron 35.0%
!! CATTGCATTTGCTTTTGTTC+TGG + Chr5:17832655-17832674 MsG0580025385.01.T01:intron 35.0%
!! GTGGTTTAATTTGGCTTGTT+GGG + Chr5:17832443-17832462 MsG0580025385.01.T01:intron 35.0%
!! TTCACAACAGGCTATTTTCA+CGG + Chr5:17832593-17832612 MsG0580025385.01.T01:intron 35.0%
AACGAGTGGGAAAAGGAAAT+AGG - Chr5:17832684-17832703 None:intergenic 40.0%
ACAACAATTCCAGCAATAGC+AGG - Chr5:17833051-17833070 None:intergenic 40.0%
ACAATGAAACGAGTGGGAAA+AGG - Chr5:17832691-17832710 None:intergenic 40.0%
ATTTGGAAACTCAGTGATGC+TGG + Chr5:17833077-17833096 MsG0580025385.01.T01:CDS 40.0%
CATCGAGTTCGTGTATCATA+TGG - Chr5:17832379-17832398 None:intergenic 40.0%
CGAAAATGAGATCGAATCAG+TGG - Chr5:17832499-17832518 None:intergenic 40.0%
GAATCTAAACCAGAAACCAC+CGG - Chr5:17833152-17833171 None:intergenic 40.0%
TGCTTTCTTGAGAGTTGTTC+CGG - Chr5:17832262-17832281 None:intergenic 40.0%
TTCCTTGCATCATTCACAAC+AGG + Chr5:17832581-17832600 MsG0580025385.01.T01:intron 40.0%
TTGTTGGACAGCTTTGCATT+AGG + Chr5:17832631-17832650 MsG0580025385.01.T01:intron 40.0%
! GGTGGTGTGATTTTCATTGT+TGG + Chr5:17832615-17832634 MsG0580025385.01.T01:intron 40.0%
! TTGGGTTGTTTCGATTTCAG+TGG + Chr5:17832424-17832443 MsG0580025385.01.T01:intron 40.0%
!! TCACAACAGGCTATTTTCAC+GGG + Chr5:17832594-17832613 MsG0580025385.01.T01:intron 40.0%
AGCAGTGACAATGAAACGAG+TGG - Chr5:17832698-17832717 None:intergenic 45.0%
AGCCTGTTGTGAATGATGCA+AGG - Chr5:17832586-17832605 None:intergenic 45.0%
ATCTTAACCACGCGCAGTAA+AGG - Chr5:17832725-17832744 None:intergenic 45.0%
CGATAATGAGAGAAGCTCTC+CGG + Chr5:17833130-17833149 MsG0580025385.01.T01:CDS 45.0%
GCAGTGACAATGAAACGAGT+GGG - Chr5:17832697-17832716 None:intergenic 45.0%
GGGTTAGATTTGTGCTCCAT+CGG + Chr5:17832904-17832923 MsG0580025385.01.T01:CDS 45.0%
TCTATTACGGACACAGTCAC+TGG + Chr5:17832808-17832827 MsG0580025385.01.T01:CDS 45.0%
TTCTCTCGCTTCTGTCTTCA+GGG + Chr5:17832778-17832797 MsG0580025385.01.T01:intron 45.0%
TTTCTCTCGCTTCTGTCTTC+AGG + Chr5:17832777-17832796 MsG0580025385.01.T01:intron 45.0%
TTTGTGCTCCATCGGAAAGA+AGG + Chr5:17832912-17832931 MsG0580025385.01.T01:CDS 45.0%
! CTTTTTGCACCTGCTATTGC+TGG + Chr5:17833039-17833058 MsG0580025385.01.T01:CDS 45.0%
! GGATTTTGCACGCTTTTGGA+AGG + Chr5:17832877-17832896 MsG0580025385.01.T01:CDS 45.0%
! GGTTCACAGTTGGTTCAGTT+GGG + Chr5:17832985-17833004 MsG0580025385.01.T01:CDS 45.0%
!! AGAAAGCACCTTCTTTCCGA+TGG - Chr5:17832923-17832942 None:intergenic 45.0%
!! GCACGCTTTTGGAAGGTTTT+GGG + Chr5:17832884-17832903 MsG0580025385.01.T01:CDS 45.0%
!! TGCACGCTTTTGGAAGGTTT+TGG + Chr5:17832883-17832902 MsG0580025385.01.T01:CDS 45.0%
!!! TACTGCTGCTGGTTTTTTGG+TGG + Chr5:17832329-17832348 MsG0580025385.01.T01:intron 45.0%
!!! TGCTACTGCTGCTGGTTTTT+TGG + Chr5:17832326-17832345 MsG0580025385.01.T01:intron 45.0%
AACGAAGTCCTAATCCGTCC+TGG - Chr5:17833011-17833030 None:intergenic 50.0%
ACGGACACAGTCACTGGAAA+TGG + Chr5:17832814-17832833 MsG0580025385.01.T01:CDS 50.0%
AGAGCTGCAGGTTTGTGTGT+CGG + Chr5:17832963-17832982 MsG0580025385.01.T01:CDS 50.0%
AGTAGCATGAAGTGAGAGGC+GGG - Chr5:17832313-17832332 None:intergenic 50.0%
CAGCAGTAGCATGAAGTGAG+AGG - Chr5:17832317-17832336 None:intergenic 50.0%
CAGTAGCATGAAGTGAGAGG+CGG - Chr5:17832314-17832333 None:intergenic 50.0%
CGGACACAGTCACTGGAAAT+GGG + Chr5:17832815-17832834 MsG0580025385.01.T01:CDS 50.0%
CTTGAGAGTTGTTCCGGTGA+AGG - Chr5:17832256-17832275 None:intergenic 50.0%
GCAAGACCTCCAATAGCATG+AGG - Chr5:17832554-17832573 None:intergenic 50.0%
GCGCGTGGTTAAGATGAACT+CGG + Chr5:17832730-17832749 MsG0580025385.01.T01:intron 50.0%
GGACACAGTCACTGGAAATG+GGG + Chr5:17832816-17832835 MsG0580025385.01.T01:CDS 50.0%
TAATGAGAGAAGCTCTCCGG+TGG + Chr5:17833133-17833152 MsG0580025385.01.T01:CDS 50.0%
TATGCTTGGATGAGAGCTGC+AGG + Chr5:17832951-17832970 MsG0580025385.01.T01:CDS 50.0%
TCACTTCATGCTACTGCTGC+TGG + Chr5:17832318-17832337 MsG0580025385.01.T01:CDS 50.0%
TCGCTTCTGTCTTCAGGGTT+TGG + Chr5:17832783-17832802 MsG0580025385.01.T01:intron 50.0%
TCGGTGAAGATGCAGATCGT+TGG + Chr5:17832749-17832768 MsG0580025385.01.T01:intron 50.0%
TGCCATTCAGAGCACATCCA+GGG + Chr5:17832856-17832875 MsG0580025385.01.T01:CDS 50.0%
TGGAAACTCAGTGATGCTGG+TGG + Chr5:17833080-17833099 MsG0580025385.01.T01:CDS 50.0%
TTGTGTGTCGGGTTCACAGT+TGG + Chr5:17832975-17832994 MsG0580025385.01.T01:CDS 50.0%
! ATCCCTGGATGTGCTCTGAA+TGG - Chr5:17832861-17832880 None:intergenic 50.0%
! CCAAAAGCGTGCAAAATCCC+TGG - Chr5:17832876-17832895 None:intergenic 50.0%
! CCAGGGATTTTGCACGCTTT+TGG + Chr5:17832873-17832892 MsG0580025385.01.T01:CDS 50.0%
! GGGTTCACAGTTGGTTCAGT+TGG + Chr5:17832984-17833003 MsG0580025385.01.T01:CDS 50.0%
!! CAACAGGCTATTTTCACGGG+TGG + Chr5:17832597-17832616 MsG0580025385.01.T01:intron 50.0%
!! GGACGGATTAGGACTTCGTT+CGG + Chr5:17833011-17833030 MsG0580025385.01.T01:CDS 50.0%
AGAAGCTCTCCGGTGGTTTC+TGG + Chr5:17833140-17833159 MsG0580025385.01.T01:CDS 55.0%
CTGCCATTCAGAGCACATCC+AGG + Chr5:17832855-17832874 MsG0580025385.01.T01:CDS 55.0%
GAGCTGCAGGTTTGTGTGTC+GGG + Chr5:17832964-17832983 MsG0580025385.01.T01:CDS 55.0%
GCTACAACCTTTACTGCGCG+TGG + Chr5:17832715-17832734 MsG0580025385.01.T01:intron 55.0%
TTGGTTCAGTTGGGCCAGGA+CGG + Chr5:17832994-17833013 MsG0580025385.01.T01:CDS 55.0%
! ACAGTTGGTTCAGTTGGGCC+AGG + Chr5:17832990-17833009 MsG0580025385.01.T01:CDS 55.0%
GAGTTGTTCCGGTGAAGGCG+CGG - Chr5:17832251-17832270 None:intergenic 60.0%
! CAGTTGGGCCAGGACGGATT+AGG + Chr5:17833000-17833019 MsG0580025385.01.T01:CDS 60.0%
!! CTGGTGGTGCTGTTGCTGCA+GGG + Chr5:17833096-17833115 MsG0580025385.01.T01:CDS 60.0%
!! GCTGGTGGTGCTGTTGCTGC+AGG + Chr5:17833095-17833114 MsG0580025385.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr5 gene 17832243 17833196 17832243 ID=MsG0580025385.01;Name=MsG0580025385.01
Chr5 mRNA 17832243 17833196 17832243 ID=MsG0580025385.01.T01;Parent=MsG0580025385.01;Name=MsG0580025385.01.T01;_AED=0.50;_eAED=0.51;_QI=0|0|0|1|1|0.5|2|0|164
Chr5 exon 17832243 17832339 17832243 ID=MsG0580025385.01.T01:exon:3007;Parent=MsG0580025385.01.T01
Chr5 exon 17832799 17833196 17832799 ID=MsG0580025385.01.T01:exon:3008;Parent=MsG0580025385.01.T01
Chr5 CDS 17832243 17832339 17832243 ID=MsG0580025385.01.T01:cds;Parent=MsG0580025385.01.T01
Chr5 CDS 17832799 17833196 17832799 ID=MsG0580025385.01.T01:cds;Parent=MsG0580025385.01.T01
Gene Sequence

>MsG0580025385.01.T01

ATGATCCGCGCCTTCACCGGAACAACTCTCAAGAAAGCACAGTTTTTCATTAGACAAGGTGTTTCATCCCGCCTCTCACTTCATGCTACTGCTGCTGGGTTTGGATTCTATTACGGACACAGTCACTGGAAATGGGGACACTTGTTCTTTTCGCTGCCATTCAGAGCACATCCAGGGATTTTGCACGCTTTTGGAAGGTTTTGGGTTAGATTTGTGCTCCATCGGAAAGAAGGTGCTTTCTCTATATGGTATGCTTGGATGAGAGCTGCAGGTTTGTGTGTCGGGTTCACAGTTGGTTCAGTTGGGCCAGGACGGATTAGGACTTCGTTCGGAGTTGCTTTTTGCACCTGCTATTGCTGGAATTGTTGTGTTGCTATTTGGAAACTCAGTGATGCTGGTGGTGCTGTTGCTGCAGGGAATGTAAGTGACGATAATGAGAGAAGCTCTCCGGTGGTTTCTGGTTTAGATTCGAAGAAGCTGCTCGAGTTTGAATAA

Protein sequence

>MsG0580025385.01.T01

MIRAFTGTTLKKAQFFIRQGVSSRLSLHATAAGFGFYYGHSHWKWGHLFFSLPFRAHPGILHAFGRFWVRFVLHRKEGAFSIWYAWMRAAGLCVGFTVGSVGPGRIRTSFGVAFCTCYCWNCCVAIWKLSDAGGAVAAGNVSDDNERSSPVVSGLDSKKLLEFE*