AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0170220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0170220 MtrunA17_Chr1g0187771 99.320 147 1 0 1 147 1 147 1.16e-108 305
Msa0170220 MtrunA17_Chr7g0261251 78.912 147 30 1 1 147 1 146 1.76e-86 249
Msa0170220 MtrunA17_Chr1g0182501 62.319 138 52 0 10 147 2 139 1.44e-66 198
Msa0170220 MtrunA17_Chr4g0037251 61.644 146 50 1 1 146 3 142 2.99e-66 197
Msa0170220 MtrunA17_Chr5g0397641 61.314 137 51 1 10 146 2 136 1.21e-63 191
Msa0170220 MtrunA17_Chr8g0386651 60.584 137 52 1 10 146 2 136 1.17e-61 186
Msa0170220 MtrunA17_Chr1g0203211 57.447 141 58 1 6 146 22 160 5.36e-60 182
Msa0170220 MtrunA17_Chr2g0291571 55.797 138 61 0 10 147 2 139 9.00e-59 179
Msa0170220 MtrunA17_Chr8g0380291 55.072 138 62 0 10 147 2 139 5.26e-56 172
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0170220 AT2G31200.1 76.190 147 34 1 1 147 1 146 6.69e-82 238
Msa0170220 AT5G59890.1 61.594 138 53 0 10 147 2 139 3.00e-65 195
Msa0170220 AT3G46010.2 57.931 145 61 0 3 147 6 150 8.65e-65 194
Msa0170220 AT4G00680.2 58.042 143 60 0 5 147 7 149 1.33e-64 194
Msa0170220 AT3G46010.1 60.145 138 55 0 10 147 2 139 2.43e-64 193
Msa0170220 AT4G00680.1 58.696 138 57 0 10 147 2 139 5.13e-64 192
Msa0170220 AT1G01750.1 58.696 138 57 0 10 147 2 139 1.95e-63 191
Msa0170220 AT1G01750.2 58.696 138 57 0 10 147 7 144 2.17e-63 191
Msa0170220 AT2G16700.1 58.503 147 55 1 1 147 3 143 3.05e-63 190
Msa0170220 AT4G25590.1 62.774 137 49 1 10 146 2 136 3.65e-63 190
Msa0170220 AT3G46000.1 60.870 138 52 1 10 147 2 137 2.68e-62 188
Msa0170220 AT3G46000.2 60.870 138 52 1 10 147 21 156 3.76e-62 188
Msa0170220 AT5G59890.3 61.364 132 51 0 16 147 1 132 2.15e-61 185
Msa0170220 AT5G59890.2 61.364 132 51 0 16 147 1 132 2.15e-61 185
Msa0170220 AT2G16700.4 61.364 132 51 0 16 147 1 132 1.13e-60 183
Msa0170220 AT2G16700.2 61.364 132 51 0 16 147 1 132 1.13e-60 183
Msa0170220 AT4G34970.1 58.394 137 57 0 11 147 5 141 2.00e-60 183
Msa0170220 AT5G52360.1 61.314 137 51 1 10 146 2 136 4.61e-60 182
Msa0170220 AT4G25590.2 62.595 131 47 1 16 146 1 129 1.72e-59 181
Msa0170220 AT5G59880.1 55.797 138 61 0 10 147 2 139 2.80e-59 180
Msa0170220 AT3G46000.3 60.606 132 50 1 16 147 1 130 1.34e-58 178
Msa0170220 AT5G59880.2 48.551 138 56 1 10 147 2 124 3.51e-46 146
Msa0170220 AT2G16700.3 50.427 117 51 2 1 117 3 112 9.61e-37 122
Msa0170220 AT3G45990.1 42.754 138 68 3 16 147 1 133 2.41e-34 117

Find 63 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCCTTAAGTACTATTATTA+TGG 0.209108 1_4:+54177654 Msa0170220:three_prime_UTR
TTGTAGGGAAATGCATCTTC+TGG 0.261866 1_4:+54176432 Msa0170220:intron
GCAGAGCGTAAGCACATTTC+AGG 0.286465 1_4:+54176470 Msa0170220:CDS
AACCATAATAATAGTACTTA+AGG 0.320026 1_4:-54177656 None:intergenic
TGTGAAGGCTGATACTGATA+TGG 0.329087 1_4:+54177736 Msa0170220:three_prime_UTR
AGAATTGTGTGGAGATGAAA+AGG 0.335598 1_4:-54174917 None:intergenic
AAGGGTTATGTCTATTAGTT+AGG 0.342272 1_4:+54177597 Msa0170220:three_prime_UTR
TGAAGAGTCAAGAATTTAGA+AGG 0.351710 1_4:-54174971 None:intergenic
GGGAATCCACTATGAGATTC+AGG 0.356002 1_4:+54177504 Msa0170220:CDS
CCTCTGCAGAGGGGTCTCTT+AGG 0.385406 1_4:-54177766 None:intergenic
AGAACCATCATATGGCAATA+AGG 0.388005 1_4:-54177802 None:intergenic
CAGGTTCAGAAGGGAGCTGC+AGG 0.400251 1_4:+54177483 Msa0170220:CDS
GAGGTCGTGGTCGAGAAAAC+TGG 0.418821 1_4:+54176552 Msa0170220:CDS
GCTTTCAGAGTGGGAGTTGG+AGG 0.419649 1_4:+54175016 Msa0170220:CDS
GTGATTAGTTCGCACGCTCC+TGG 0.421098 1_4:-54177561 None:intergenic
TGTTCCTTATTGCCATATGA+TGG 0.447267 1_4:+54177798 Msa0170220:three_prime_UTR
ACCATAATAATAGTACTTAA+GGG 0.452507 1_4:-54177655 None:intergenic
GATGTAGCATAAAGCATCTT+CGG 0.454388 1_4:-54177454 None:intergenic
ACTTCGAGTTCCATCTCTGT+TGG 0.461999 1_4:-54177535 None:intergenic
ATTGCCATATGATGGTTCTC+AGG 0.462653 1_4:+54177806 Msa0170220:three_prime_UTR
CTTCGAGTTCCATCTCTGTT+GGG 0.469550 1_4:-54177534 None:intergenic
CTGTAGCAGCTTCATAGCTA+TGG 0.485371 1_4:+54174994 Msa0170220:exon
ATAACCCTTTCAGTTTGCTC+AGG 0.490136 1_4:-54177583 None:intergenic
TGTAGGGAAATGCATCTTCT+GGG 0.504974 1_4:+54176433 Msa0170220:intron
GGATATTGAAACTCTGTCAA+TGG 0.511931 1_4:+54177852 Msa0170220:three_prime_UTR
CTTTCAGAGTGGGAGTTGGA+GGG 0.516133 1_4:+54175017 Msa0170220:CDS
ATCACCTGAGCAAACTGAAA+GGG 0.520857 1_4:+54177579 Msa0170220:exon
TCCACCTGAGAACCATCATA+TGG 0.526072 1_4:-54177810 None:intergenic
ATGGCTTTCAGAGTGGGAGT+TGG 0.530306 1_4:+54175013 Msa0170220:CDS
TTTCAGAGTGGGAGTTGGAG+GGG 0.532929 1_4:+54175018 Msa0170220:CDS
ATAGCTATGGCTTTCAGAGT+GGG 0.535262 1_4:+54175007 Msa0170220:exon
GGGAAATGCATCTTCTGGGA+TGG 0.538512 1_4:+54176437 Msa0170220:intron
CATCAAAGGACAGGTTCAGA+AGG 0.548452 1_4:+54177473 Msa0170220:CDS
AGCTAAAATTCCTCTGCAGA+GGG 0.551878 1_4:-54177776 None:intergenic
TTGTAGAGAGAGAAAGTGAG+AGG 0.554165 1_4:-54174946 None:intergenic
GGAAATGCATCTTCTGGGAT+GGG 0.555903 1_4:+54176438 Msa0170220:CDS
GGCAACAGACCCAACAGAGA+TGG 0.557284 1_4:+54177525 Msa0170220:CDS
GATGGAACTCGAAGTCCTCC+AGG 0.560122 1_4:+54177543 Msa0170220:CDS
AAATCATCATAGCTCTCAGA+AGG 0.561770 1_4:-54176579 None:intergenic
GTGAAGGCTGATACTGATAT+GGG 0.562558 1_4:+54177737 Msa0170220:three_prime_UTR
GGACGAATACGAGCTACAGA+AGG 0.568172 1_4:-54177433 None:intergenic
CATAGCTATGGCTTTCAGAG+TGG 0.573358 1_4:+54175006 Msa0170220:exon
TAAACAACATTCACCTGTGA+AGG 0.573509 1_4:+54177721 Msa0170220:three_prime_UTR
GAATTGTGTGGAGATGAAAA+GGG 0.573731 1_4:-54174916 None:intergenic
AGAGGAGAGAGAGAATTGTG+TGG 0.574529 1_4:-54174928 None:intergenic
CTGTTGCCTGAATCTCATAG+TGG 0.585469 1_4:-54177510 None:intergenic
TTTATGCTACATCAAAGGAC+AGG 0.592719 1_4:+54177464 Msa0170220:CDS
ATATCAGTATCAGCCTTCAC+AGG 0.593659 1_4:-54177734 None:intergenic
GATGCTTTATGCTACATCAA+AGG 0.601779 1_4:+54177459 Msa0170220:CDS
CAGCTAAAATTCCTCTGCAG+AGG 0.602388 1_4:-54177777 None:intergenic
ACTCTGTCAATGGAGGTGTT+TGG 0.602656 1_4:+54177862 Msa0170220:three_prime_UTR
AATCACCTGAGCAAACTGAA+AGG 0.609728 1_4:+54177578 Msa0170220:exon
TTTGTTGAAAACATTTCTTG+AGG 0.612163 1_4:-54177630 None:intergenic
ATCAAAGGACAGGTTCAGAA+GGG 0.621100 1_4:+54177474 Msa0170220:CDS
CCTAAGAGACCCCTCTGCAG+AGG 0.626974 1_4:+54177766 Msa0170220:three_prime_UTR
GCCATATGATGGTTCTCAGG+TGG 0.634844 1_4:+54177809 Msa0170220:three_prime_UTR
GCACATTTCAGGAATTGCAG+AGG 0.661602 1_4:+54176481 Msa0170220:CDS
ATTAGTTCGCACGCTCCTGG+AGG 0.663424 1_4:-54177558 None:intergenic
GTCGTGGTCGAGAAAACTGG+AGG 0.680018 1_4:+54176555 Msa0170220:CDS
GCTAAAATTCCTCTGCAGAG+GGG 0.687024 1_4:-54177775 None:intergenic
AGGTTCAGAAGGGAGCTGCA+GGG 0.689968 1_4:+54177484 Msa0170220:CDS
TATTGAAACTCTGTCAATGG+AGG 0.714240 1_4:+54177855 Msa0170220:three_prime_UTR
AATCATCATAGCTCTCAGAA+GGG 0.724970 1_4:-54176578 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATGGTTAAAAAAATATTTT+AGG - chr1_4:54174859-54174878 None:intergenic 10.0%
!! ATTTGAATAAAAATTTCTGA+GGG + chr1_4:54175475-54175494 Msa0170220:intron 15.0%
!! AACACAAAAATGAATAAAAA+AGG - chr1_4:54177000-54177019 None:intergenic 15.0%
!!! TTAATTAGTTTATATACTGT+AGG + chr1_4:54177038-54177057 Msa0170220:intron 15.0%
!! AATAAATGATAGAATGAAGA+TGG - chr1_4:54175051-54175070 None:intergenic 20.0%
!!! TAAGTTTTTATTTTGCTTTG+TGG + chr1_4:54175201-54175220 Msa0170220:intron 20.0%
!! GCATATGATTGAAAAATATA+TGG - chr1_4:54175268-54175287 None:intergenic 20.0%
!!! AAATGTAGACAAATTTCTTT+TGG - chr1_4:54175459-54175478 None:intergenic 20.0%
!! CATTTGAATAAAAATTTCTG+AGG + chr1_4:54175474-54175493 Msa0170220:intron 20.0%
!!! AAATAAATATCTTCGCATTT+TGG - chr1_4:54175605-54175624 None:intergenic 20.0%
!! ACTTAATAATCATATCAATG+GGG - chr1_4:54175870-54175889 None:intergenic 20.0%
!! CACTTAATAATCATATCAAT+GGG - chr1_4:54175871-54175890 None:intergenic 20.0%
!! ACACTTAATAATCATATCAA+TGG - chr1_4:54175872-54175891 None:intergenic 20.0%
!! TTGATATGATTATTAAGTGT+TGG + chr1_4:54175872-54175891 Msa0170220:intron 20.0%
!! TGATATGATTATTAAGTGTT+GGG + chr1_4:54175873-54175892 Msa0170220:intron 20.0%
!!! TTTCTCATTTTATACTTTAG+AGG + chr1_4:54175996-54176015 Msa0170220:intron 20.0%
!!! GATTATATTGCTTTGAAAAA+AGG + chr1_4:54176376-54176395 Msa0170220:intron 20.0%
!!! TAAAAAAGATTTTGCTCTTT+TGG - chr1_4:54176677-54176696 None:intergenic 20.0%
!! ACATATGTATATATTGCATT+TGG + chr1_4:54176792-54176811 Msa0170220:intron 20.0%
!!! TTTTGTGTTGATTTTTCTTT+TGG + chr1_4:54177011-54177030 Msa0170220:intron 20.0%
!!! TTTGTGTTGATTTTTCTTTT+GGG + chr1_4:54177012-54177031 Msa0170220:intron 20.0%
!! GACAATCTTAAAAAAAACTT+TGG + chr1_4:54177127-54177146 Msa0170220:intron 20.0%
!!! TTTTATTTTCTGCTTACATA+TGG + chr1_4:54177262-54177281 Msa0170220:intron 20.0%
!! ACCATAATAATAGTACTTAA+GGG - chr1_4:54177658-54177677 None:intergenic 20.0%
!! AACCATAATAATAGTACTTA+AGG - chr1_4:54177659-54177678 None:intergenic 20.0%
!!! GAAGTAATATGCTTATTTTA+TGG + chr1_4:54177831-54177850 Msa0170220:three_prime_UTR 20.0%
! TTAGACCTAAAATTTGTAGT+GGG - chr1_4:54175184-54175203 None:intergenic 25.0%
!!! GTTCATTTTTGTGTTGTTTT+TGG + chr1_4:54175515-54175534 Msa0170220:intron 25.0%
!!! TTTTTGTGTTGTTTTTGGTT+TGG + chr1_4:54175520-54175539 Msa0170220:intron 25.0%
!!! TTTTGTGTTGTTTTTGGTTT+GGG + chr1_4:54175521-54175540 Msa0170220:intron 25.0%
! TCCAATGATGTTAAGAAATT+AGG + chr1_4:54175638-54175657 Msa0170220:intron 25.0%
! TTATATATTGCTACTAGAGT+CGG - chr1_4:54175810-54175829 None:intergenic 25.0%
! AAGTTAAATGTAGATAGTGT+AGG + chr1_4:54175962-54175981 Msa0170220:intron 25.0%
!!! TATTGCTTTGAAAAAAGGAT+TGG + chr1_4:54176381-54176400 Msa0170220:intron 25.0%
! AATTTCGTGTAACTAATATG+TGG + chr1_4:54176404-54176423 Msa0170220:intron 25.0%
!!! TAATATGTGGATTTGTTGTA+GGG + chr1_4:54176417-54176436 Msa0170220:intron 25.0%
! AATTGATGAGAAAAAGAAAG+AGG + chr1_4:54176533-54176552 Msa0170220:CDS 25.0%
! AATAAAAAAGGTTGAGAGAT+AGG - chr1_4:54176988-54177007 None:intergenic 25.0%
! ATTTATATCCATCAGAATCA+TGG + chr1_4:54177094-54177113 Msa0170220:intron 25.0%
!!! ATTTTTTTGCAATCCACTAT+TGG - chr1_4:54177169-54177188 None:intergenic 25.0%
! ATTATAGTATTGTAGCGTAA+CGG - chr1_4:54177209-54177228 None:intergenic 25.0%
! AGTAAACTCAAGCAAAATAA+AGG - chr1_4:54177389-54177408 None:intergenic 25.0%
!! ATTTTGCTTGAGTTTACTAA+TGG + chr1_4:54177391-54177410 Msa0170220:intron 25.0%
! TTTGTTGAAAACATTTCTTG+AGG - chr1_4:54177633-54177652 None:intergenic 25.0%
!! CATTTTTGCGAATTTAGCTT+GGG + chr1_4:54174875-54174894 Msa0170220:five_prime_UTR 30.0%
!!! ATTTTTGCGAATTTAGCTTG+GGG + chr1_4:54174876-54174895 Msa0170220:five_prime_UTR 30.0%
TGAAGAGTCAAGAATTTAGA+AGG - chr1_4:54174974-54174993 None:intergenic 30.0%
AAATCAAACCAAAATCAGCA+TGG - chr1_4:54175101-54175120 None:intergenic 30.0%
!!! TCGAATGTTGTTGTTTTTGT+CGG + chr1_4:54175123-54175142 Msa0170220:intron 30.0%
!!! TTGTTTTTGTCGGATTTTGT+TGG + chr1_4:54175133-54175152 Msa0170220:intron 30.0%
! TTCTTCCCACTACAAATTTT+AGG + chr1_4:54175176-54175195 Msa0170220:intron 30.0%
CTTAGACCTAAAATTTGTAG+TGG - chr1_4:54175185-54175204 None:intergenic 30.0%
AAGAAAAACCACATAATACG+AGG - chr1_4:54175233-54175252 None:intergenic 30.0%
CATGTCTTTAGTACTTTGTT+GGG + chr1_4:54175550-54175569 Msa0170220:intron 30.0%
GCCTAATTTCTTAACATCAT+TGG - chr1_4:54175642-54175661 None:intergenic 30.0%
TTGAATGTAGTTACATGTGT+CGG + chr1_4:54175834-54175853 Msa0170220:intron 30.0%
AGAAGTTTATGACTACACTT+CGG - chr1_4:54176030-54176049 None:intergenic 30.0%
! TACAGTTTCCTCTTTTTGAT+TGG + chr1_4:54176178-54176197 Msa0170220:intron 30.0%
CTAAATCACCAATCAAAAAG+AGG - chr1_4:54176189-54176208 None:intergenic 30.0%
TATAATCAAAGTCCTTGTGA+TGG - chr1_4:54176363-54176382 None:intergenic 30.0%
!! CTAATATGTGGATTTGTTGT+AGG + chr1_4:54176416-54176435 Msa0170220:intron 30.0%
! GCATATCTACAATCATTTTC+AGG - chr1_4:54176618-54176637 None:intergenic 30.0%
!!! GCAAAATCTTTTTTATCGCA+TGG + chr1_4:54176682-54176701 Msa0170220:CDS 30.0%
! TTGCTCAATTTTTCTTGCAT+TGG + chr1_4:54176750-54176769 Msa0170220:intron 30.0%
!! AGTGGATTGCAAAAAAATAG+CGG + chr1_4:54177171-54177190 Msa0170220:intron 30.0%
TGTCAAATTATAACTTCCTC+TGG - chr1_4:54177354-54177373 None:intergenic 30.0%
! CTAATGGATAATTGCTTTAC+AGG + chr1_4:54177407-54177426 Msa0170220:intron 30.0%
AAGGGTTATGTCTATTAGTT+AGG + chr1_4:54177597-54177616 Msa0170220:three_prime_UTR 30.0%
GCCCTTAAGTACTATTATTA+TGG + chr1_4:54177654-54177673 Msa0170220:three_prime_UTR 30.0%
GAACTAGAAAAATACGAAAC+AGG - chr1_4:54177904-54177923 None:intergenic 30.0%
CCAAGCTAAATTCGCAAAAA+TGG - chr1_4:54174877-54174896 None:intergenic 35.0%
!! CCATTTTTGCGAATTTAGCT+TGG + chr1_4:54174874-54174893 Msa0170220:five_prime_UTR 35.0%
!!! TTTTTGCGAATTTAGCTTGG+GGG + chr1_4:54174877-54174896 Msa0170220:five_prime_UTR 35.0%
GAATTGTGTGGAGATGAAAA+GGG - chr1_4:54174919-54174938 None:intergenic 35.0%
AGAATTGTGTGGAGATGAAA+AGG - chr1_4:54174920-54174939 None:intergenic 35.0%
CTTACAGACATCTTCAACAT+CGG - chr1_4:54175317-54175336 None:intergenic 35.0%
ACACACAATATCAGCATTGT+CGG - chr1_4:54175352-54175371 None:intergenic 35.0%
! CAAAGTACTAAAGACATGCA+AGG - chr1_4:54175548-54175567 None:intergenic 35.0%
GCATGTCTTTAGTACTTTGT+TGG + chr1_4:54175549-54175568 Msa0170220:intron 35.0%
TTGTCAAATGAAATCCTGTG+CGG + chr1_4:54175668-54175687 Msa0170220:intron 35.0%
!! ACTTCATTTTTCCTCAAGCA+TGG + chr1_4:54175692-54175711 Msa0170220:intron 35.0%
!! GATTATTAAGTGTTGGGATC+CGG + chr1_4:54175879-54175898 Msa0170220:intron 35.0%
TTATGACTACACTTCGGATT+TGG - chr1_4:54176024-54176043 None:intergenic 35.0%
!!! TTTTTTGCACACTTACTGCA+TGG + chr1_4:54176124-54176143 Msa0170220:intron 35.0%
AGGAAACTGTAAATATTCCC+GGG - chr1_4:54176169-54176188 None:intergenic 35.0%
GAGGAAACTGTAAATATTCC+CGG - chr1_4:54176170-54176189 None:intergenic 35.0%
ATGAAATGAATCCCAGCAAT+GGG - chr1_4:54176306-54176325 None:intergenic 35.0%
CGAAAGGACTTTAGTATCAA+TGG + chr1_4:54176326-54176345 Msa0170220:intron 35.0%
AATCATCATAGCTCTCAGAA+GGG - chr1_4:54176581-54176600 None:intergenic 35.0%
AAATCATCATAGCTCTCAGA+AGG - chr1_4:54176582-54176601 None:intergenic 35.0%
!! TCTACAATCATTTTCAGGCA+AGG - chr1_4:54176613-54176632 None:intergenic 35.0%
AAAGACAGAAATCTGAACGA+GGG + chr1_4:54176869-54176888 Msa0170220:intron 35.0%
TTTATATACTGTAGGCATCC+TGG + chr1_4:54177046-54177065 Msa0170220:intron 35.0%
TAAACTAGTGTTGCCAATAG+TGG + chr1_4:54177153-54177172 Msa0170220:intron 35.0%
TTCTCTGCAAGCAGAAAATA+GGG - chr1_4:54177234-54177253 None:intergenic 35.0%
TTTCTCTGCAAGCAGAAAAT+AGG - chr1_4:54177235-54177254 None:intergenic 35.0%
! TTTTCTGCTTACATATGGAG+AGG + chr1_4:54177267-54177286 Msa0170220:intron 35.0%
! GATGTAGCATAAAGCATCTT+CGG - chr1_4:54177457-54177476 None:intergenic 35.0%
GATGCTTTATGCTACATCAA+AGG + chr1_4:54177459-54177478 Msa0170220:CDS 35.0%
TTTATGCTACATCAAAGGAC+AGG + chr1_4:54177464-54177483 Msa0170220:CDS 35.0%
TAAACAACATTCACCTGTGA+AGG + chr1_4:54177721-54177740 Msa0170220:three_prime_UTR 35.0%
TGTTCCTTATTGCCATATGA+TGG + chr1_4:54177798-54177817 Msa0170220:three_prime_UTR 35.0%
AGAACCATCATATGGCAATA+AGG - chr1_4:54177805-54177824 None:intergenic 35.0%
GGATATTGAAACTCTGTCAA+TGG + chr1_4:54177852-54177871 Msa0170220:three_prime_UTR 35.0%
TATTGAAACTCTGTCAATGG+AGG + chr1_4:54177855-54177874 Msa0170220:three_prime_UTR 35.0%
TTGTAGAGAGAGAAAGTGAG+AGG - chr1_4:54174949-54174968 None:intergenic 40.0%
ATAGCTATGGCTTTCAGAGT+GGG + chr1_4:54175007-54175026 Msa0170220:exon 40.0%
! CCAAAATCAGCATGGTCTTA+AGG - chr1_4:54175093-54175112 None:intergenic 40.0%
!!! CCTTAAGACCATGCTGATTT+TGG + chr1_4:54175090-54175109 Msa0170220:intron 40.0%
! GGTTGATTCCTCGTATTATG+TGG + chr1_4:54175222-54175241 Msa0170220:intron 40.0%
CGATGTTGAAGATGTCTGTA+AGG + chr1_4:54175315-54175334 Msa0170220:intron 40.0%
TGTGTTAGACACTTTCACTC+TGG + chr1_4:54175367-54175386 Msa0170220:intron 40.0%
TCTTTAGTACTTTGTTGGGC+TGG + chr1_4:54175554-54175573 Msa0170220:intron 40.0%
GTTACAAGTAGACATGACAC+CGG + chr1_4:54175726-54175745 Msa0170220:intron 40.0%
! GAATTGCTCACTAGAGTATC+CGG - chr1_4:54175901-54175920 None:intergenic 40.0%
GTAAGATATATCTCATGCCC+CGG + chr1_4:54176148-54176167 Msa0170220:intron 40.0%
TAAGATATATCTCATGCCCC+GGG + chr1_4:54176149-54176168 Msa0170220:intron 40.0%
GGAAACTGTAAATATTCCCG+GGG - chr1_4:54176168-54176187 None:intergenic 40.0%
!! TGTTTGGCAGAGCATTCTTT+TGG - chr1_4:54176273-54176292 None:intergenic 40.0%
AATGGGGAAAACAGCATGTT+TGG - chr1_4:54176289-54176308 None:intergenic 40.0%
TGAAATGAATCCCAGCAATG+GGG - chr1_4:54176305-54176324 None:intergenic 40.0%
GATGAAATGAATCCCAGCAA+TGG - chr1_4:54176307-54176326 None:intergenic 40.0%
CTGGGATTCATTTCATCGAA+AGG + chr1_4:54176310-54176329 Msa0170220:intron 40.0%
GAATCTCATCATCCATCACA+AGG + chr1_4:54176348-54176367 Msa0170220:intron 40.0%
TTGTAGGGAAATGCATCTTC+TGG + chr1_4:54176432-54176451 Msa0170220:intron 40.0%
TGTAGGGAAATGCATCTTCT+GGG + chr1_4:54176433-54176452 Msa0170220:intron 40.0%
TGAGAAAAAGAAAGAGGTCG+TGG + chr1_4:54176539-54176558 Msa0170220:CDS 40.0%
CTCGTTCAGATTTCTGTCTT+TGG - chr1_4:54176870-54176889 None:intergenic 40.0%
CAAAGACAGAAATCTGAACG+AGG + chr1_4:54176868-54176887 Msa0170220:intron 40.0%
TCTTGAGACCATGATTCTGA+TGG - chr1_4:54177105-54177124 None:intergenic 40.0%
GCAAAGTGTGAAAAGAGAGA+TGG - chr1_4:54177293-54177312 None:intergenic 40.0%
AACGCAACACGGTAAATGAA+AGG - chr1_4:54177328-54177347 None:intergenic 40.0%
ATCAAAGGACAGGTTCAGAA+GGG + chr1_4:54177474-54177493 Msa0170220:CDS 40.0%
AATCACCTGAGCAAACTGAA+AGG + chr1_4:54177578-54177597 Msa0170220:exon 40.0%
ATCACCTGAGCAAACTGAAA+GGG + chr1_4:54177579-54177598 Msa0170220:exon 40.0%
! ATAACCCTTTCAGTTTGCTC+AGG - chr1_4:54177586-54177605 None:intergenic 40.0%
ATATCAGTATCAGCCTTCAC+AGG - chr1_4:54177737-54177756 None:intergenic 40.0%
TGTGAAGGCTGATACTGATA+TGG + chr1_4:54177736-54177755 Msa0170220:three_prime_UTR 40.0%
GTGAAGGCTGATACTGATAT+GGG + chr1_4:54177737-54177756 Msa0170220:three_prime_UTR 40.0%
AGCTAAAATTCCTCTGCAGA+GGG - chr1_4:54177779-54177798 None:intergenic 40.0%
ATTGCCATATGATGGTTCTC+AGG + chr1_4:54177806-54177825 Msa0170220:three_prime_UTR 40.0%
AGAGGAGAGAGAGAATTGTG+TGG - chr1_4:54174931-54174950 None:intergenic 45.0%
CTGTAGCAGCTTCATAGCTA+TGG + chr1_4:54174994-54175013 Msa0170220:exon 45.0%
CATAGCTATGGCTTTCAGAG+TGG + chr1_4:54175006-54175025 Msa0170220:exon 45.0%
!! GTCGGATTTTGTTGGTTGCA+TGG + chr1_4:54175141-54175160 Msa0170220:intron 45.0%
! CAGCATTGTCGGTTGAGATA+GGG - chr1_4:54175341-54175360 None:intergenic 45.0%
! TCAGCATTGTCGGTTGAGAT+AGG - chr1_4:54175342-54175361 None:intergenic 45.0%
!! GGAAAAATGAAGTACCGCAC+AGG - chr1_4:54175685-54175704 None:intergenic 45.0%
TCAAAGCAACTCCATGCTTG+AGG - chr1_4:54175706-54175725 None:intergenic 45.0%
!! CATAGAAAGTGTCCAGTGTC+CGG - chr1_4:54175748-54175767 None:intergenic 45.0%
AACCTGTGAAGCACATACAC+TGG + chr1_4:54175776-54175795 Msa0170220:intron 45.0%
!!! ATGCTGTTTTCCCCATTGCT+GGG + chr1_4:54176292-54176311 Msa0170220:intron 45.0%
!! GGAAATGCATCTTCTGGGAT+GGG + chr1_4:54176438-54176457 Msa0170220:CDS 45.0%
GCACATTTCAGGAATTGCAG+AGG + chr1_4:54176481-54176500 Msa0170220:CDS 45.0%
AGATTGTCGATCTTGAGACC+AGG - chr1_4:54177067-54177086 None:intergenic 45.0%
! TCCTCTGGTTTAACGCAACA+CGG - chr1_4:54177339-54177358 None:intergenic 45.0%
CATCAAAGGACAGGTTCAGA+AGG + chr1_4:54177473-54177492 Msa0170220:CDS 45.0%
GGGAATCCACTATGAGATTC+AGG + chr1_4:54177504-54177523 Msa0170220:CDS 45.0%
CTGTTGCCTGAATCTCATAG+TGG - chr1_4:54177513-54177532 None:intergenic 45.0%
! CTTCGAGTTCCATCTCTGTT+GGG - chr1_4:54177537-54177556 None:intergenic 45.0%
! ACTTCGAGTTCCATCTCTGT+TGG - chr1_4:54177538-54177557 None:intergenic 45.0%
GCTAAAATTCCTCTGCAGAG+GGG - chr1_4:54177778-54177797 None:intergenic 45.0%
CAGCTAAAATTCCTCTGCAG+AGG - chr1_4:54177780-54177799 None:intergenic 45.0%
TCCACCTGAGAACCATCATA+TGG - chr1_4:54177813-54177832 None:intergenic 45.0%
!! ACTCTGTCAATGGAGGTGTT+TGG + chr1_4:54177862-54177881 Msa0170220:three_prime_UTR 45.0%
ATGGCTTTCAGAGTGGGAGT+TGG + chr1_4:54175013-54175032 Msa0170220:CDS 50.0%
CTTTCAGAGTGGGAGTTGGA+GGG + chr1_4:54175017-54175036 Msa0170220:CDS 50.0%
TTTCAGAGTGGGAGTTGGAG+GGG + chr1_4:54175018-54175037 Msa0170220:CDS 50.0%
CGGACACTGGACACTTTCTA+TGG + chr1_4:54175746-54175765 Msa0170220:intron 50.0%
GTCCAGTGTATGTGCTTCAC+AGG - chr1_4:54175781-54175800 None:intergenic 50.0%
!!! CATGCTGTTTTCCCCATTGC+TGG + chr1_4:54176291-54176310 Msa0170220:intron 50.0%
! GGGAAATGCATCTTCTGGGA+TGG + chr1_4:54176437-54176456 Msa0170220:intron 50.0%
GCAGAGCGTAAGCACATTTC+AGG + chr1_4:54176470-54176489 Msa0170220:CDS 50.0%
ACCGTGTTGCGTTAAACCAG+AGG + chr1_4:54177335-54177354 Msa0170220:intron 50.0%
GGACGAATACGAGCTACAGA+AGG - chr1_4:54177436-54177455 None:intergenic 50.0%
GCCATATGATGGTTCTCAGG+TGG + chr1_4:54177809-54177828 Msa0170220:three_prime_UTR 50.0%
!! GCGAATTTAGCTTGGGGGAC+TGG + chr1_4:54174882-54174901 Msa0170220:five_prime_UTR 55.0%
GCTTTCAGAGTGGGAGTTGG+AGG + chr1_4:54175016-54175035 Msa0170220:CDS 55.0%
GTAGACATGACACCGGACAC+TGG + chr1_4:54175733-54175752 Msa0170220:intron 55.0%
GAGGTCGTGGTCGAGAAAAC+TGG + chr1_4:54176552-54176571 Msa0170220:CDS 55.0%
GTCGTGGTCGAGAAAACTGG+AGG + chr1_4:54176555-54176574 Msa0170220:CDS 55.0%
AGGTTCAGAAGGGAGCTGCA+GGG + chr1_4:54177484-54177503 Msa0170220:CDS 55.0%
GGCAACAGACCCAACAGAGA+TGG + chr1_4:54177525-54177544 Msa0170220:CDS 55.0%
GATGGAACTCGAAGTCCTCC+AGG + chr1_4:54177543-54177562 Msa0170220:CDS 55.0%
ATTAGTTCGCACGCTCCTGG+AGG - chr1_4:54177561-54177580 None:intergenic 55.0%
! GTGATTAGTTCGCACGCTCC+TGG - chr1_4:54177564-54177583 None:intergenic 55.0%
CAGGTTCAGAAGGGAGCTGC+AGG + chr1_4:54177483-54177502 Msa0170220:CDS 60.0%
CCTCTGCAGAGGGGTCTCTT+AGG - chr1_4:54177769-54177788 None:intergenic 60.0%
CCTAAGAGACCCCTCTGCAG+AGG + chr1_4:54177766-54177785 Msa0170220:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 54174846 54177940 54174846 ID=Msa0170220;Name=Msa0170220
chr1_4 mRNA 54174846 54177940 54174846 ID=Msa0170220-mRNA-1;Parent=Msa0170220;Name=Msa0170220-mRNA-1;_AED=0.02;_eAED=0.02;_QI=167|1|1|1|1|1|3|361|147
chr1_4 exon 54174846 54175039 54174846 ID=Msa0170220-mRNA-1:exon:16111;Parent=Msa0170220-mRNA-1
chr1_4 exon 54176438 54176703 54176438 ID=Msa0170220-mRNA-1:exon:16112;Parent=Msa0170220-mRNA-1
chr1_4 exon 54177429 54177940 54177429 ID=Msa0170220-mRNA-1:exon:16113;Parent=Msa0170220-mRNA-1
chr1_4 five_prime_UTR 54174846 54175012 54174846 ID=Msa0170220-mRNA-1:five_prime_utr;Parent=Msa0170220-mRNA-1
chr1_4 CDS 54175013 54175039 54175013 ID=Msa0170220-mRNA-1:cds;Parent=Msa0170220-mRNA-1
chr1_4 CDS 54176438 54176703 54176438 ID=Msa0170220-mRNA-1:cds;Parent=Msa0170220-mRNA-1
chr1_4 CDS 54177429 54177579 54177429 ID=Msa0170220-mRNA-1:cds;Parent=Msa0170220-mRNA-1
chr1_4 three_prime_UTR 54177580 54177940 54177580 ID=Msa0170220-mRNA-1:three_prime_utr;Parent=Msa0170220-mRNA-1
Gene Sequence

>Msa0170220

ATGGCTTTCAGAGTGGGAGTTGGAGGGGGAAATGCATCTTCTGGGATGGGTGTTGCTGAGCAGAGCGTAAGCACATTTCAGGAATTGCAGAGGAAGAAAGTATATCGCTATGTGATTTTCAAAATTGATGAGAAAAAGAAAGAGGTCGTGGTCGAGAAAACTGGAGGCCCTTCTGAGAGCTATGATGATTTCACTGCATCCTTGCCTGAAAATGATTGTAGATATGCAGTCTTTGACTTTGATTTCGTGACTGCTGAGAACTGCCAAAAGAGCAAAATCTTTTTTATCGCATGGTCTCCTTCTGTAGCTCGTATTCGTCCGAAGATGCTTTATGCTACATCAAAGGACAGGTTCAGAAGGGAGCTGCAGGGAATCCACTATGAGATTCAGGCAACAGACCCAACAGAGATGGAACTCGAAGTCCTCCAGGAGCGTGCGAACTAA
Protein sequence

>Msa0170220

MAFRVGVGGGNASSGMGVAEQSVSTFQELQRKKVYRYVIFKIDEKKKEVVVEKTGGPSESYDDFTASLPENDCRYAVFDFDFVTAENCQKSKIFFIAWSPSVARIRPKMLYATSKDRFRRELQGIHYEIQATDPTEMELEVLQERAN*