AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0031120

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0031120 MtrunA17_Chr1g0188981 95.789 190 7 1 1 190 1 189 1.27e-131 367
Msa0031120 MtrunA17_Chr6g0458331 40.351 114 65 2 28 141 36 146 9.27e-26 97.4
Msa0031120 MtrunA17_Chr4g0062341 34.132 167 91 7 26 187 29 181 1.67e-24 94.7
Msa0031120 MtrunA17_Chr4g0062311 34.132 167 91 7 26 187 29 181 1.67e-24 94.7
Msa0031120 MtrunA17_Chr1g0205701 34.177 158 84 3 9 152 8 159 5.62e-23 90.9
Msa0031120 MtrunA17_Chr4g0039721 31.902 163 92 5 26 184 29 176 3.87e-22 88.6
Msa0031120 MtrunA17_Chr1g0150131 38.776 98 59 1 32 129 29 125 1.77e-21 86.7
Msa0031120 MtrunA17_Chr2g0331181 32.895 152 92 3 6 152 7 153 1.87e-20 84.3
Msa0031120 MtrunA17_Chr3g0136871 33.333 162 94 6 32 191 31 180 1.13e-19 82.0
Msa0031120 MtrunA17_Chr5g0394321 32.099 162 95 4 11 171 7 154 2.43e-19 81.3
Msa0031120 MtrunA17_Chr2g0324721 32.886 149 92 3 26 174 24 164 4.60e-19 80.9
Msa0031120 MtrunA17_Chr4g0040291 35.200 125 76 3 28 150 24 145 7.20e-19 80.1
Msa0031120 MtrunA17_Chr4g0041811 35.514 107 68 1 23 129 26 131 1.14e-18 80.1
Msa0031120 MtrunA17_Chr2g0324711 35.246 122 73 2 8 129 7 122 1.42e-18 82.0
Msa0031120 MtrunA17_Chr2g0319611 36.111 108 68 1 23 129 20 127 2.26e-17 77.4
Msa0031120 MtrunA17_Chr8g0385041 38.655 119 68 3 27 145 23 136 6.48e-17 77.0
Msa0031120 MtrunA17_Chr8g0360371 28.689 122 84 1 8 129 3 121 9.49e-17 76.3
Msa0031120 MtrunA17_Chr2g0323021 32.836 134 75 5 32 156 29 156 3.99e-16 73.6
Msa0031120 MtrunA17_Chr8g0385031 33.051 118 76 2 13 129 7 122 2.25e-15 72.4
Msa0031120 MtrunA17_Chr2g0317231 32.759 116 69 4 26 138 22 131 1.13e-14 71.6
Msa0031120 MtrunA17_Chr6g0481491 28.226 124 82 3 7 129 5 122 2.85e-14 68.2
Msa0031120 MtrunA17_Chr4g0064041 30.345 145 84 4 13 152 8 140 7.85e-14 68.6
Msa0031120 MtrunA17_Chr4g0064041 32.673 101 67 1 29 129 191 290 1.00e-12 65.5
Msa0031120 MtrunA17_Chr8g0385021 33.051 118 76 2 13 129 7 122 9.78e-14 67.8
Msa0031120 MtrunA17_Chr8g0387804 33.333 99 64 1 31 129 31 127 1.35e-13 64.7
Msa0031120 MtrunA17_Chr1g0196801 31.915 141 85 5 29 169 23 152 1.95e-13 65.5
Msa0031120 MtrunA17_Chr4g0073901 29.126 103 71 2 28 129 28 129 8.37e-13 63.9
Msa0031120 MtrunA17_Chr6g0459441 33.028 109 66 4 23 130 20 122 9.45e-12 60.8
Msa0031120 MtrunA17_Chr8g0335571 32.381 105 65 2 25 129 20 118 1.41e-11 60.8
Msa0031120 MtrunA17_Chr8g0335531 32.075 106 66 2 24 129 19 118 3.26e-11 59.7
Msa0031120 MtrunA17_Chr1g0185541 37.879 66 41 0 64 129 5 70 4.70e-11 56.6
Msa0031120 MtrunA17_Chr3g0132411 31.683 101 63 2 29 129 65 159 6.06e-11 58.5
Msa0031120 MtrunA17_Chr1g0211101 27.344 128 87 2 8 134 12 134 6.62e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0031120 AT1G64640.1 55.280 161 60 4 9 162 6 161 5.94e-54 170
Msa0031120 AT3G20570.1 38.793 116 69 1 14 129 15 128 9.10e-27 101
Msa0031120 AT1G79800.1 38.843 121 71 2 28 147 33 151 3.48e-22 89.4
Msa0031120 AT1G48940.1 38.318 107 63 2 24 129 21 125 6.27e-22 88.2
Msa0031120 AT3G18590.1 37.864 103 61 2 28 129 26 126 1.11e-20 85.1
Msa0031120 AT4G31840.1 38.400 125 72 3 32 153 31 153 1.47e-20 84.7
Msa0031120 AT2G25060.1 39.000 100 57 2 30 129 36 131 1.56e-20 84.7
Msa0031120 AT5G57920.1 37.755 98 60 1 32 129 27 123 2.65e-20 84.0
Msa0031120 AT5G57920.2 37.755 98 60 1 32 129 26 122 3.96e-20 83.6
Msa0031120 AT5G53870.2 35.152 165 95 6 8 169 12 167 5.07e-20 86.3
Msa0031120 AT5G53870.1 35.152 165 95 6 8 169 12 167 6.41e-20 86.3
Msa0031120 AT5G57920.3 37.755 98 60 1 32 129 59 155 9.14e-20 83.6
Msa0031120 AT4G27520.1 32.432 148 95 3 12 158 14 157 3.90e-19 84.0
Msa0031120 AT4G28365.1 32.867 143 87 5 27 166 27 163 5.72e-19 80.9
Msa0031120 AT5G14345.1 32.061 131 83 2 14 144 5 129 7.48e-19 79.3
Msa0031120 AT4G32490.1 33.333 108 69 1 27 134 29 133 2.01e-18 80.1
Msa0031120 AT5G25090.1 31.967 122 81 2 9 129 6 126 2.73e-18 79.0
Msa0031120 AT2G32300.2 28.049 164 110 2 1 164 31 186 5.41e-18 80.1
Msa0031120 AT2G32300.1 28.049 164 110 2 1 164 1 156 5.86e-18 79.3
Msa0031120 AT4G30590.1 35.714 98 62 1 32 129 32 128 1.92e-17 76.6
Msa0031120 AT2G23990.1 37.374 99 60 2 32 129 31 128 2.69e-17 76.6
Msa0031120 AT5G26330.1 34.014 147 74 3 29 155 24 167 8.35e-17 75.1
Msa0031120 AT2G31050.1 32.857 140 87 4 5 142 6 140 1.11e-12 64.3
Msa0031120 AT2G23990.2 30.508 118 61 3 32 129 31 147 1.24e-12 64.3
Msa0031120 AT5G07475.1 30.400 125 72 4 31 155 32 141 1.91e-12 63.5
Msa0031120 AT1G45063.2 33.333 108 68 2 29 135 149 253 2.18e-12 64.3
Msa0031120 AT1G45063.1 33.333 108 68 2 29 135 149 253 2.77e-12 64.7
Msa0031120 AT2G26720.1 27.778 144 98 2 1 142 1 140 8.84e-12 62.0
Msa0031120 AT3G60270.1 31.132 106 67 2 24 129 21 120 4.32e-11 59.7
Msa0031120 AT3G17675.1 30.303 99 64 1 31 129 34 127 4.90e-11 58.2

Find 48 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGGGTGAAGTTGTGAAATT+TGG 0.223732 1_1:+57694111 None:intergenic
CATATATCTGTTGGAGGAAT+TGG 0.263666 1_1:-57693768 Msa0031120:CDS
CCATTGTTCATAAACAATAT+TGG 0.281321 1_1:+57693876 None:intergenic
TTGATTATGTAATACTTTGT+TGG 0.307301 1_1:-57693428 Msa0031120:three_prime_UTR
AAAACACTTTGGTTTGGATT+TGG 0.318460 1_1:+57694212 None:intergenic
CCCTCTTCACAAACAGTGTT+TGG 0.328717 1_1:-57693693 Msa0031120:CDS
TACTTCACAAGTGGAGAAAA+TGG 0.332320 1_1:-57693816 Msa0031120:CDS
ATCCACAAGTCTATGCTAAA+TGG 0.353275 1_1:-57694135 Msa0031120:CDS
CAGTCATAACAACTGGTTCT+TGG 0.354742 1_1:-57694260 Msa0031120:CDS
GTTGTTATGACTGGCCATTT+TGG 0.356548 1_1:+57694270 None:intergenic
GAAAATAATAGAAACCAAAA+TGG 0.373724 1_1:-57694284 Msa0031120:exon
ACACATGAAAAGTTGAAGTT+AGG 0.397508 1_1:+57693631 None:intergenic
TTAGATCTCCAACTTTGAAT+TGG 0.398002 1_1:+57694185 None:intergenic
GGTAGCAAAACACTTTGGTT+TGG 0.401653 1_1:+57694206 None:intergenic
AGTTGGAGATCTAAATGCTT+GGG 0.404972 1_1:-57694176 Msa0031120:CDS
TCAATCTGTAATTCTTTATG+TGG 0.412274 1_1:-57693478 Msa0031120:three_prime_UTR
CCAAACACTGTTTGTGAAGA+GGG 0.433432 1_1:+57693693 None:intergenic
TTTGATGAAGAAGGAGCTAC+TGG 0.443782 1_1:+57693663 None:intergenic
CAAAAGATTCATATATCTGT+TGG 0.454335 1_1:-57693777 Msa0031120:CDS
TGAGGTGAATTTGATGAAGA+AGG 0.457613 1_1:+57693654 None:intergenic
AAATTTCACAACTTCACCCT+TGG 0.467456 1_1:-57694109 Msa0031120:CDS
AAATGGCCAGTCATAACAAC+TGG 0.486950 1_1:-57694267 Msa0031120:CDS
TTCCGATTGAGTCAAAATAG+AGG 0.500670 1_1:+57693386 None:intergenic
TGTGAAGAGGGTGCAGATGC+AGG 0.517498 1_1:+57693705 None:intergenic
AACAAAGTATTACATAATCA+AGG 0.522050 1_1:+57693430 None:intergenic
GACCATTTAGCATAGACTTG+TGG 0.528971 1_1:+57694133 None:intergenic
ATCAACGAATCTTGACTTGG+TGG 0.531393 1_1:+57693942 None:intergenic
AATGTTGATGCTGAAGCTAA+TGG 0.532427 1_1:-57693744 Msa0031120:CDS
GAATTGGTAGCAAAACACTT+TGG 0.533380 1_1:+57694201 None:intergenic
CCAATATTGTTTATGAACAA+TGG 0.534060 1_1:-57693876 Msa0031120:CDS
GATGCAGGCAATGAAGAACT+TGG 0.536171 1_1:+57693720 None:intergenic
AAGTTGGAGATCTAAATGCT+TGG 0.539050 1_1:-57694177 Msa0031120:CDS
TCCGATTGAGTCAAAATAGA+GGG 0.540503 1_1:+57693387 None:intergenic
TCCAAACACTGTTTGTGAAG+AGG 0.548927 1_1:+57693692 None:intergenic
AAGATTCATATATCTGTTGG+AGG 0.558686 1_1:-57693774 Msa0031120:CDS
GACTTGTGGATTTGCTGAGG+TGG 0.563923 1_1:+57694147 None:intergenic
CAAGAACCAGTTGTTATGAC+TGG 0.565931 1_1:+57694261 None:intergenic
TGAATCAACGAATCTTGACT+TGG 0.567574 1_1:+57693939 None:intergenic
GGTGATTTCTACTTCACAAG+TGG 0.570982 1_1:-57693825 Msa0031120:CDS
ACTTACAAAGTGAGTCTCCA+AGG 0.579525 1_1:+57694092 None:intergenic
ACTTGTGGATTTGCTGAGGT+GGG 0.610232 1_1:+57694148 None:intergenic
GTTGGAGATCTAAATGCTTG+GGG 0.615224 1_1:-57694175 Msa0031120:CDS
TTGTTTAACATAACATCACA+TGG 0.618399 1_1:-57693846 Msa0031120:CDS
TGAAAAGTTGAAGTTAGGTG+AGG 0.664125 1_1:+57693636 None:intergenic
ATAGACTTGTGGATTTGCTG+AGG 0.672234 1_1:+57694144 None:intergenic
CTTACAAAGTGAGTCTCCAA+GGG 0.672775 1_1:+57694093 None:intergenic
CGATTGAGTCAAAATAGAGG+GGG 0.679259 1_1:+57693389 None:intergenic
CCGATTGAGTCAAAATAGAG+GGG 0.680707 1_1:+57693388 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTACATTAAAGTAAAAAAT+AGG + chr1_1:57694070-57694089 None:intergenic 10.0%
!! TTTACATTAAAGTAAAAAAT+AGG + chr1_1:57694070-57694089 None:intergenic 10.0%
!!! TCTATTATTTTCTTAGATTT+AGG + chr1_1:57693359-57693378 None:intergenic 15.0%
!!! TCTATTATTTTCTTAGATTT+AGG + chr1_1:57693359-57693378 None:intergenic 15.0%
!! GAAAATAATAGAAACCAAAA+TGG - chr1_1:57693367-57693386 Msa0031120:three_prime_UTR 20.0%
!! GTTGGAATATATTGTATATA+TGG + chr1_1:57693585-57693604 None:intergenic 20.0%
!! TAAAATGAAATTAAGGAAGT+TGG + chr1_1:57693603-57693622 None:intergenic 20.0%
!! GTAACACTAAAATGAAATTA+AGG + chr1_1:57693610-57693629 None:intergenic 20.0%
!!! TATGAATCTTTTGATTCTTT+TGG + chr1_1:57693867-57693886 None:intergenic 20.0%
!!! ACTTTTCATGTGTTTATTAT+TGG - chr1_1:57694030-57694049 Msa0031120:intron 20.0%
!!! AACAAAGTATTACATAATCA+AGG + chr1_1:57694224-57694243 None:intergenic 20.0%
!! TTGATTATGTAATACTTTGT+TGG - chr1_1:57694223-57694242 Msa0031120:CDS 20.0%
!!! AAATTCCGAGTTTTAAAATA+GGG + chr1_1:57694300-57694319 None:intergenic 20.0%
!!! AAAATTCCGAGTTTTAAAAT+AGG + chr1_1:57694301-57694320 None:intergenic 20.0%
!! ATTCCGAAAAACATTAAATT+GGG + chr1_1:57694329-57694348 None:intergenic 20.0%
!! AATTCCGAAAAACATTAAAT+TGG + chr1_1:57694330-57694349 None:intergenic 20.0%
!! GAAAATAATAGAAACCAAAA+TGG - chr1_1:57693367-57693386 Msa0031120:three_prime_UTR 20.0%
!! GTTGGAATATATTGTATATA+TGG + chr1_1:57693585-57693604 None:intergenic 20.0%
!! TAAAATGAAATTAAGGAAGT+TGG + chr1_1:57693603-57693622 None:intergenic 20.0%
!! GTAACACTAAAATGAAATTA+AGG + chr1_1:57693610-57693629 None:intergenic 20.0%
!!! TATGAATCTTTTGATTCTTT+TGG + chr1_1:57693867-57693886 None:intergenic 20.0%
!!! ACTTTTCATGTGTTTATTAT+TGG - chr1_1:57694030-57694049 Msa0031120:intron 20.0%
!!! AACAAAGTATTACATAATCA+AGG + chr1_1:57694224-57694243 None:intergenic 20.0%
!! TTGATTATGTAATACTTTGT+TGG - chr1_1:57694223-57694242 Msa0031120:CDS 20.0%
!!! AAATTCCGAGTTTTAAAATA+GGG + chr1_1:57694300-57694319 None:intergenic 20.0%
!!! AAAATTCCGAGTTTTAAAAT+AGG + chr1_1:57694301-57694320 None:intergenic 20.0%
!! ATTCCGAAAAACATTAAATT+GGG + chr1_1:57694329-57694348 None:intergenic 20.0%
!! AATTCCGAAAAACATTAAAT+TGG + chr1_1:57694330-57694349 None:intergenic 20.0%
! TAGTGTTACAAAACTCAAAA+AGG - chr1_1:57693621-57693640 Msa0031120:CDS 25.0%
! AGTGTTACAAAACTCAAAAA+GGG - chr1_1:57693622-57693641 Msa0031120:CDS 25.0%
! CCATTGTTCATAAACAATAT+TGG + chr1_1:57693778-57693797 None:intergenic 25.0%
! CCAATATTGTTTATGAACAA+TGG - chr1_1:57693775-57693794 Msa0031120:CDS 25.0%
! TTGTTTAACATAACATCACA+TGG - chr1_1:57693805-57693824 Msa0031120:CDS 25.0%
! CAAAAGATTCATATATCTGT+TGG - chr1_1:57693874-57693893 Msa0031120:CDS 25.0%
! TCAATCTGTAATTCTTTATG+TGG - chr1_1:57694173-57694192 Msa0031120:CDS 25.0%
!!! AATTCCGAGTTTTAAAATAG+GGG + chr1_1:57694299-57694318 None:intergenic 25.0%
! TTCCGAAAAACATTAAATTG+GGG + chr1_1:57694328-57694347 None:intergenic 25.0%
! TAGTGTTACAAAACTCAAAA+AGG - chr1_1:57693621-57693640 Msa0031120:CDS 25.0%
! AGTGTTACAAAACTCAAAAA+GGG - chr1_1:57693622-57693641 Msa0031120:CDS 25.0%
! CCATTGTTCATAAACAATAT+TGG + chr1_1:57693778-57693797 None:intergenic 25.0%
! CCAATATTGTTTATGAACAA+TGG - chr1_1:57693775-57693794 Msa0031120:CDS 25.0%
! TTGTTTAACATAACATCACA+TGG - chr1_1:57693805-57693824 Msa0031120:CDS 25.0%
! CAAAAGATTCATATATCTGT+TGG - chr1_1:57693874-57693893 Msa0031120:CDS 25.0%
! TCAATCTGTAATTCTTTATG+TGG - chr1_1:57694173-57694192 Msa0031120:CDS 25.0%
!!! AATTCCGAGTTTTAAAATAG+GGG + chr1_1:57694299-57694318 None:intergenic 25.0%
! TTCCGAAAAACATTAAATTG+GGG + chr1_1:57694328-57694347 None:intergenic 25.0%
!! AAAACACTTTGGTTTGGATT+TGG + chr1_1:57693442-57693461 None:intergenic 30.0%
! TTTTGCTACCAATTCAAAGT+TGG - chr1_1:57693458-57693477 Msa0031120:three_prime_UTR 30.0%
TTAGATCTCCAACTTTGAAT+TGG + chr1_1:57693469-57693488 None:intergenic 30.0%
AAGATTCATATATCTGTTGG+AGG - chr1_1:57693877-57693896 Msa0031120:CDS 30.0%
ACACATGAAAAGTTGAAGTT+AGG + chr1_1:57694023-57694042 None:intergenic 30.0%
!!! ATTCCGAGTTTTAAAATAGG+GGG + chr1_1:57694298-57694317 None:intergenic 30.0%
TCCGAAAAACATTAAATTGG+GGG + chr1_1:57694327-57694346 None:intergenic 30.0%
!! AAAACACTTTGGTTTGGATT+TGG + chr1_1:57693442-57693461 None:intergenic 30.0%
! TTTTGCTACCAATTCAAAGT+TGG - chr1_1:57693458-57693477 Msa0031120:three_prime_UTR 30.0%
TTAGATCTCCAACTTTGAAT+TGG + chr1_1:57693469-57693488 None:intergenic 30.0%
AAGATTCATATATCTGTTGG+AGG - chr1_1:57693877-57693896 Msa0031120:CDS 30.0%
ACACATGAAAAGTTGAAGTT+AGG + chr1_1:57694023-57694042 None:intergenic 30.0%
!!! ATTCCGAGTTTTAAAATAGG+GGG + chr1_1:57694298-57694317 None:intergenic 30.0%
TCCGAAAAACATTAAATTGG+GGG + chr1_1:57694327-57694346 None:intergenic 30.0%
CCAATAAAAAGTCCTTGTTG+CGG - chr1_1:57693328-57693347 Msa0031120:three_prime_UTR 35.0%
CAATAAAAAGTCCTTGTTGC+GGG - chr1_1:57693329-57693348 Msa0031120:three_prime_UTR 35.0%
AATAAAAAGTCCTTGTTGCG+GGG - chr1_1:57693330-57693349 Msa0031120:three_prime_UTR 35.0%
! GAATTGGTAGCAAAACACTT+TGG + chr1_1:57693453-57693472 None:intergenic 35.0%
AAGTTGGAGATCTAAATGCT+TGG - chr1_1:57693474-57693493 Msa0031120:three_prime_UTR 35.0%
AGTTGGAGATCTAAATGCTT+GGG - chr1_1:57693475-57693494 Msa0031120:three_prime_UTR 35.0%
ATCCACAAGTCTATGCTAAA+TGG - chr1_1:57693516-57693535 Msa0031120:three_prime_UTR 35.0%
AAGGGTGAAGTTGTGAAATT+TGG + chr1_1:57693543-57693562 None:intergenic 35.0%
AAATTTCACAACTTCACCCT+TGG - chr1_1:57693542-57693561 Msa0031120:three_prime_UTR 35.0%
TGAATCAACGAATCTTGACT+TGG + chr1_1:57693715-57693734 None:intergenic 35.0%
TACTTCACAAGTGGAGAAAA+TGG - chr1_1:57693835-57693854 Msa0031120:CDS 35.0%
CATATATCTGTTGGAGGAAT+TGG - chr1_1:57693883-57693902 Msa0031120:CDS 35.0%
AATGTTGATGCTGAAGCTAA+TGG - chr1_1:57693907-57693926 Msa0031120:CDS 35.0%
TGAGGTGAATTTGATGAAGA+AGG + chr1_1:57694000-57694019 None:intergenic 35.0%
TGAAAAGTTGAAGTTAGGTG+AGG + chr1_1:57694018-57694037 None:intergenic 35.0%
TCCGATTGAGTCAAAATAGA+GGG + chr1_1:57694267-57694286 None:intergenic 35.0%
TTCCGATTGAGTCAAAATAG+AGG + chr1_1:57694268-57694287 None:intergenic 35.0%
!!! TTCCGAGTTTTAAAATAGGG+GGG + chr1_1:57694297-57694316 None:intergenic 35.0%
CGAAAAACATTAAATTGGGG+GGG + chr1_1:57694325-57694344 None:intergenic 35.0%
CCGAAAAACATTAAATTGGG+GGG + chr1_1:57694326-57694345 None:intergenic 35.0%
! CCCCCCAATTTAATGTTTTT+CGG - chr1_1:57694323-57694342 Msa0031120:five_prime_UTR 35.0%
CCAATAAAAAGTCCTTGTTG+CGG - chr1_1:57693328-57693347 Msa0031120:three_prime_UTR 35.0%
CAATAAAAAGTCCTTGTTGC+GGG - chr1_1:57693329-57693348 Msa0031120:three_prime_UTR 35.0%
AATAAAAAGTCCTTGTTGCG+GGG - chr1_1:57693330-57693349 Msa0031120:three_prime_UTR 35.0%
! GAATTGGTAGCAAAACACTT+TGG + chr1_1:57693453-57693472 None:intergenic 35.0%
AAGTTGGAGATCTAAATGCT+TGG - chr1_1:57693474-57693493 Msa0031120:three_prime_UTR 35.0%
AGTTGGAGATCTAAATGCTT+GGG - chr1_1:57693475-57693494 Msa0031120:three_prime_UTR 35.0%
ATCCACAAGTCTATGCTAAA+TGG - chr1_1:57693516-57693535 Msa0031120:three_prime_UTR 35.0%
AAGGGTGAAGTTGTGAAATT+TGG + chr1_1:57693543-57693562 None:intergenic 35.0%
AAATTTCACAACTTCACCCT+TGG - chr1_1:57693542-57693561 Msa0031120:three_prime_UTR 35.0%
TGAATCAACGAATCTTGACT+TGG + chr1_1:57693715-57693734 None:intergenic 35.0%
TACTTCACAAGTGGAGAAAA+TGG - chr1_1:57693835-57693854 Msa0031120:CDS 35.0%
CATATATCTGTTGGAGGAAT+TGG - chr1_1:57693883-57693902 Msa0031120:CDS 35.0%
AATGTTGATGCTGAAGCTAA+TGG - chr1_1:57693907-57693926 Msa0031120:CDS 35.0%
TGAGGTGAATTTGATGAAGA+AGG + chr1_1:57694000-57694019 None:intergenic 35.0%
TGAAAAGTTGAAGTTAGGTG+AGG + chr1_1:57694018-57694037 None:intergenic 35.0%
TCCGATTGAGTCAAAATAGA+GGG + chr1_1:57694267-57694286 None:intergenic 35.0%
TTCCGATTGAGTCAAAATAG+AGG + chr1_1:57694268-57694287 None:intergenic 35.0%
!!! TTCCGAGTTTTAAAATAGGG+GGG + chr1_1:57694297-57694316 None:intergenic 35.0%
CGAAAAACATTAAATTGGGG+GGG + chr1_1:57694325-57694344 None:intergenic 35.0%
CCGAAAAACATTAAATTGGG+GGG + chr1_1:57694326-57694345 None:intergenic 35.0%
! CCCCCCAATTTAATGTTTTT+CGG - chr1_1:57694323-57694342 Msa0031120:five_prime_UTR 35.0%
! CCGCAACAAGGACTTTTTAT+TGG + chr1_1:57693331-57693350 None:intergenic 40.0%
ATAAAAAGTCCTTGTTGCGG+GGG - chr1_1:57693331-57693350 Msa0031120:three_prime_UTR 40.0%
!! GTTGTTATGACTGGCCATTT+TGG + chr1_1:57693384-57693403 None:intergenic 40.0%
AAATGGCCAGTCATAACAAC+TGG - chr1_1:57693384-57693403 Msa0031120:three_prime_UTR 40.0%
CAAGAACCAGTTGTTATGAC+TGG + chr1_1:57693393-57693412 None:intergenic 40.0%
! CAGTCATAACAACTGGTTCT+TGG - chr1_1:57693391-57693410 Msa0031120:three_prime_UTR 40.0%
GGTAGCAAAACACTTTGGTT+TGG + chr1_1:57693448-57693467 None:intergenic 40.0%
GTTGGAGATCTAAATGCTTG+GGG - chr1_1:57693476-57693495 Msa0031120:three_prime_UTR 40.0%
!! ATAGACTTGTGGATTTGCTG+AGG + chr1_1:57693510-57693529 None:intergenic 40.0%
GACCATTTAGCATAGACTTG+TGG + chr1_1:57693521-57693540 None:intergenic 40.0%
CTTACAAAGTGAGTCTCCAA+GGG + chr1_1:57693561-57693580 None:intergenic 40.0%
ACTTACAAAGTGAGTCTCCA+AGG + chr1_1:57693562-57693581 None:intergenic 40.0%
!! ATCAACGAATCTTGACTTGG+TGG + chr1_1:57693712-57693731 None:intergenic 40.0%
GGTGATTTCTACTTCACAAG+TGG - chr1_1:57693826-57693845 Msa0031120:CDS 40.0%
CCAAACACTGTTTGTGAAGA+GGG + chr1_1:57693961-57693980 None:intergenic 40.0%
TCCAAACACTGTTTGTGAAG+AGG + chr1_1:57693962-57693981 None:intergenic 40.0%
TTTGATGAAGAAGGAGCTAC+TGG + chr1_1:57693991-57694010 None:intergenic 40.0%
CGATTGAGTCAAAATAGAGG+GGG + chr1_1:57694265-57694284 None:intergenic 40.0%
CCGATTGAGTCAAAATAGAG+GGG + chr1_1:57694266-57694285 None:intergenic 40.0%
!!! CCCCTCTATTTTGACTCAAT+CGG - chr1_1:57694263-57694282 Msa0031120:CDS 40.0%
!!! ACCCCCCCTATTTTAAAACT+CGG - chr1_1:57694292-57694311 Msa0031120:five_prime_UTR 40.0%
!!! TCCGAGTTTTAAAATAGGGG+GGG + chr1_1:57694296-57694315 None:intergenic 40.0%
! CCGCAACAAGGACTTTTTAT+TGG + chr1_1:57693331-57693350 None:intergenic 40.0%
ATAAAAAGTCCTTGTTGCGG+GGG - chr1_1:57693331-57693350 Msa0031120:three_prime_UTR 40.0%
!! GTTGTTATGACTGGCCATTT+TGG + chr1_1:57693384-57693403 None:intergenic 40.0%
AAATGGCCAGTCATAACAAC+TGG - chr1_1:57693384-57693403 Msa0031120:three_prime_UTR 40.0%
CAAGAACCAGTTGTTATGAC+TGG + chr1_1:57693393-57693412 None:intergenic 40.0%
! CAGTCATAACAACTGGTTCT+TGG - chr1_1:57693391-57693410 Msa0031120:three_prime_UTR 40.0%
GGTAGCAAAACACTTTGGTT+TGG + chr1_1:57693448-57693467 None:intergenic 40.0%
GTTGGAGATCTAAATGCTTG+GGG - chr1_1:57693476-57693495 Msa0031120:three_prime_UTR 40.0%
!! ATAGACTTGTGGATTTGCTG+AGG + chr1_1:57693510-57693529 None:intergenic 40.0%
GACCATTTAGCATAGACTTG+TGG + chr1_1:57693521-57693540 None:intergenic 40.0%
CTTACAAAGTGAGTCTCCAA+GGG + chr1_1:57693561-57693580 None:intergenic 40.0%
ACTTACAAAGTGAGTCTCCA+AGG + chr1_1:57693562-57693581 None:intergenic 40.0%
!! ATCAACGAATCTTGACTTGG+TGG + chr1_1:57693712-57693731 None:intergenic 40.0%
GGTGATTTCTACTTCACAAG+TGG - chr1_1:57693826-57693845 Msa0031120:CDS 40.0%
CCAAACACTGTTTGTGAAGA+GGG + chr1_1:57693961-57693980 None:intergenic 40.0%
TCCAAACACTGTTTGTGAAG+AGG + chr1_1:57693962-57693981 None:intergenic 40.0%
TTTGATGAAGAAGGAGCTAC+TGG + chr1_1:57693991-57694010 None:intergenic 40.0%
CGATTGAGTCAAAATAGAGG+GGG + chr1_1:57694265-57694284 None:intergenic 40.0%
CCGATTGAGTCAAAATAGAG+GGG + chr1_1:57694266-57694285 None:intergenic 40.0%
!!! CCCCTCTATTTTGACTCAAT+CGG - chr1_1:57694263-57694282 Msa0031120:CDS 40.0%
!!! ACCCCCCCTATTTTAAAACT+CGG - chr1_1:57694292-57694311 Msa0031120:five_prime_UTR 40.0%
!!! TCCGAGTTTTAAAATAGGGG+GGG + chr1_1:57694296-57694315 None:intergenic 40.0%
TAAAAAGTCCTTGTTGCGGG+GGG - chr1_1:57693332-57693351 Msa0031120:three_prime_UTR 45.0%
!! ACTTGTGGATTTGCTGAGGT+GGG + chr1_1:57693506-57693525 None:intergenic 45.0%
GATGCAGGCAATGAAGAACT+TGG + chr1_1:57693934-57693953 None:intergenic 45.0%
CCCTCTTCACAAACAGTGTT+TGG - chr1_1:57693958-57693977 Msa0031120:intron 45.0%
TAAAAAGTCCTTGTTGCGGG+GGG - chr1_1:57693332-57693351 Msa0031120:three_prime_UTR 45.0%
!! ACTTGTGGATTTGCTGAGGT+GGG + chr1_1:57693506-57693525 None:intergenic 45.0%
GATGCAGGCAATGAAGAACT+TGG + chr1_1:57693934-57693953 None:intergenic 45.0%
CCCTCTTCACAAACAGTGTT+TGG - chr1_1:57693958-57693977 Msa0031120:intron 45.0%
!! GACTTGTGGATTTGCTGAGG+TGG + chr1_1:57693507-57693526 None:intergenic 50.0%
!! GACTTGTGGATTTGCTGAGG+TGG + chr1_1:57693507-57693526 None:intergenic 50.0%
! ATTTAGGACCCCCCGCAACA+AGG + chr1_1:57693343-57693362 None:intergenic 55.0%
! TGTGAAGAGGGTGCAGATGC+AGG + chr1_1:57693949-57693968 None:intergenic 55.0%
! ATTTAGGACCCCCCGCAACA+AGG + chr1_1:57693343-57693362 None:intergenic 55.0%
! TGTGAAGAGGGTGCAGATGC+AGG + chr1_1:57693949-57693968 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 57693321 57694352 57693321 ID=Msa0031120;Name=Msa0031120
chr1_1 mRNA 57693321 57694352 57693321 ID=Msa0031120-mRNA-1;Parent=Msa0031120;Name=Msa0031120-mRNA-1;_AED=0.04;_eAED=0.04;_QI=65|1|1|1|1|1|2|269|191
chr1_1 exon 57693321 57693975 57693321 ID=Msa0031120-mRNA-1:exon:17465;Parent=Msa0031120-mRNA-1
chr1_1 exon 57694098 57694352 57694098 ID=Msa0031120-mRNA-1:exon:17464;Parent=Msa0031120-mRNA-1
chr1_1 five_prime_UTR 57694288 57694352 57694288 ID=Msa0031120-mRNA-1:five_prime_utr;Parent=Msa0031120-mRNA-1
chr1_1 CDS 57694098 57694287 57694098 ID=Msa0031120-mRNA-1:cds;Parent=Msa0031120-mRNA-1
chr1_1 CDS 57693590 57693975 57693590 ID=Msa0031120-mRNA-1:cds;Parent=Msa0031120-mRNA-1
chr1_1 three_prime_UTR 57693321 57693589 57693321 ID=Msa0031120-mRNA-1:three_prime_utr;Parent=Msa0031120-mRNA-1
Gene Sequence

>Msa0031120

ATGGCCAGTCATAACAACTGGTTCTTGGTTTCTTCTCTCTTGCTTACATTTCTCCAAATCCAAACCAAAGTGTTTTGCTACCAATTCAAAGTTGGAGATCTAAATGCTTGGGGTATACCCACCTCAGCAAATCCACAAGTCTATGCTAAATGGTCCAAATTTCACAACTTCACCCTTGGAGACTCACTTTTGTTTCTATATCCACCAAGTCAAGATTCGTTGATTCAAGTAACACAAGAATCTTACAAGAGCTGCAACACTAAAGATCCAATATTGTTTATGAACAATGGTAACTCATTGTTTAACATAACATCACATGGTGATTTCTACTTCACAAGTGGAGAAAATGGTCATTGCCAAAAGAATCAAAAGATTCATATATCTGTTGGAGGAATTGGAAATGTTGATGCTGAAGCTAATGGTCCAAGTTCTTCATTGCCTGCATCTGCACCCTCTTCACAAACAGTGTTTGGAAACATTCCAGTAGCTCCTTCTTCATCAAATTCACCTCACCTAACTTCAACTTTTCATGTGTTTATTATTGGATCTCTCATATATGCCTATTTTTTACTTTAA
Protein sequence

>Msa0031120

MASHNNWFLVSSLLLTFLQIQTKVFCYQFKVGDLNAWGIPTSANPQVYAKWSKFHNFTLGDSLLFLYPPSQDSLIQVTQESYKSCNTKDPILFMNNGNSLFNITSHGDFYFTSGENGHCQKNQKIHISVGGIGNVDAEANGPSSSLPASAPSSQTVFGNIPVAPSSSNSPHLTSTFHVFIIGSLIYAYFLL*