AlfalfaGEDB | Msa0031120

M. sativa cultivar zhongmu-4 / Msa0031120

KEGG: -
GO: -
PFAM: PF02298.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0031120	sp\|Q9SK27\|ENL1_ARATH	39.000	100	57	2	30	129	36	131	1.54e-19	84.7

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0031080	Msa0031120	0.918696	1.132627e-86	-8.615850e-47
Msa0031120	Msa0078390	0.838428	2.893111e-57	-8.615850e-47
Msa0031120	Msa0094960	0.817991	2.474468e-52	-8.615850e-47
Msa0031120	Msa0126130	0.876781	1.062743e-68	-8.615850e-47
Msa0031120	Msa0171060	0.857117	2.029990e-62	-8.615850e-47
Msa0031120	Msa0260250	0.814209	1.731137e-51	-8.615850e-47
Msa0031120	Msa0260290	0.814920	1.204626e-51	-8.615850e-47
Msa0031120	Msa0304250	0.819163	1.341853e-52	-8.615850e-47
Msa0031120	Msa0349550	0.806165	9.390466e-50	-8.615850e-47
Msa0031120	Msa0368640	0.809663	1.692907e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0031120	MtrunA17_Chr1g0188981	95.789	190	7	1	1	190	1	189	1.27e-131	367
Msa0031120	MtrunA17_Chr6g0458331	40.351	114	65	2	28	141	36	146	9.27e-26	97.4
Msa0031120	MtrunA17_Chr4g0062341	34.132	167	91	7	26	187	29	181	1.67e-24	94.7
Msa0031120	MtrunA17_Chr4g0062311	34.132	167	91	7	26	187	29	181	1.67e-24	94.7
Msa0031120	MtrunA17_Chr1g0205701	34.177	158	84	3	9	152	8	159	5.62e-23	90.9
Msa0031120	MtrunA17_Chr4g0039721	31.902	163	92	5	26	184	29	176	3.87e-22	88.6
Msa0031120	MtrunA17_Chr1g0150131	38.776	98	59	1	32	129	29	125	1.77e-21	86.7
Msa0031120	MtrunA17_Chr2g0331181	32.895	152	92	3	6	152	7	153	1.87e-20	84.3
Msa0031120	MtrunA17_Chr3g0136871	33.333	162	94	6	32	191	31	180	1.13e-19	82.0
Msa0031120	MtrunA17_Chr5g0394321	32.099	162	95	4	11	171	7	154	2.43e-19	81.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0031120	AT1G64640.1	55.280	161	60	4	9	162	6	161	5.94e-54	170
Msa0031120	AT3G20570.1	38.793	116	69	1	14	129	15	128	9.10e-27	101
Msa0031120	AT1G79800.1	38.843	121	71	2	28	147	33	151	3.48e-22	89.4
Msa0031120	AT1G48940.1	38.318	107	63	2	24	129	21	125	6.27e-22	88.2
Msa0031120	AT3G18590.1	37.864	103	61	2	28	129	26	126	1.11e-20	85.1
Msa0031120	AT4G31840.1	38.400	125	72	3	32	153	31	153	1.47e-20	84.7
Msa0031120	AT2G25060.1	39.000	100	57	2	30	129	36	131	1.56e-20	84.7
Msa0031120	AT5G57920.1	37.755	98	60	1	32	129	27	123	2.65e-20	84.0
Msa0031120	AT5G57920.2	37.755	98	60	1	32	129	26	122	3.96e-20	83.6
Msa0031120	AT5G53870.2	35.152	165	95	6	8	169	12	167	5.07e-20	86.3

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Find 48 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACACTTTGGTTTGGATT+TGG	0.318460	1_1:+57694212	None:intergenic
AAATGGCCAGTCATAACAAC+TGG	0.486950	1_1:-57694267	Msa0031120:CDS
AAATTTCACAACTTCACCCT+TGG	0.467456	1_1:-57694109	Msa0031120:CDS
AACAAAGTATTACATAATCA+AGG	0.522050	1_1:+57693430	None:intergenic
AAGATTCATATATCTGTTGG+AGG	0.558686	1_1:-57693774	Msa0031120:CDS
AAGGGTGAAGTTGTGAAATT+TGG	0.223732	1_1:+57694111	None:intergenic
AAGTTGGAGATCTAAATGCT+TGG	0.539050	1_1:-57694177	Msa0031120:CDS
AATGTTGATGCTGAAGCTAA+TGG	0.532427	1_1:-57693744	Msa0031120:CDS
ACACATGAAAAGTTGAAGTT+AGG	0.397508	1_1:+57693631	None:intergenic
ACTTACAAAGTGAGTCTCCA+AGG	0.579525	1_1:+57694092	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTAGATCTCCAACTTTGAAT+TGG	+	chr1_1:57693469-57693488	None:intergenic	30.0%
AAGATTCATATATCTGTTGG+AGG	-	chr1_1:57693877-57693896	Msa0031120:CDS	30.0%
ACACATGAAAAGTTGAAGTT+AGG	+	chr1_1:57694023-57694042	None:intergenic	30.0%
TCCGAAAAACATTAAATTGG+GGG	+	chr1_1:57694327-57694346	None:intergenic	30.0%
TTAGATCTCCAACTTTGAAT+TGG	+	chr1_1:57693469-57693488	None:intergenic	30.0%
AAGATTCATATATCTGTTGG+AGG	-	chr1_1:57693877-57693896	Msa0031120:CDS	30.0%
ACACATGAAAAGTTGAAGTT+AGG	+	chr1_1:57694023-57694042	None:intergenic	30.0%
TCCGAAAAACATTAAATTGG+GGG	+	chr1_1:57694327-57694346	None:intergenic	30.0%
CCAATAAAAAGTCCTTGTTG+CGG	-	chr1_1:57693328-57693347	Msa0031120:three_prime_UTR	35.0%
CAATAAAAAGTCCTTGTTGC+GGG	-	chr1_1:57693329-57693348	Msa0031120:three_prime_UTR	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	57693321	57694352	57693321	ID=Msa0031120;Name=Msa0031120
chr1_1	mRNA	57693321	57694352	57693321	ID=Msa0031120-mRNA-1;Parent=Msa0031120;Name=Msa0031120-mRNA-1;_AED=0.04;_eAED=0.04;_QI=65\|1\|1\|1\|1\|1\|2\|269\|191
chr1_1	exon	57693321	57693975	57693321	ID=Msa0031120-mRNA-1:exon:17465;Parent=Msa0031120-mRNA-1
chr1_1	exon	57694098	57694352	57694098	ID=Msa0031120-mRNA-1:exon:17464;Parent=Msa0031120-mRNA-1
chr1_1	five_prime_UTR	57694288	57694352	57694288	ID=Msa0031120-mRNA-1:five_prime_utr;Parent=Msa0031120-mRNA-1
chr1_1	CDS	57694098	57694287	57694098	ID=Msa0031120-mRNA-1:cds;Parent=Msa0031120-mRNA-1
chr1_1	CDS	57693590	57693975	57693590	ID=Msa0031120-mRNA-1:cds;Parent=Msa0031120-mRNA-1
chr1_1	three_prime_UTR	57693321	57693589	57693321	ID=Msa0031120-mRNA-1:three_prime_utr;Parent=Msa0031120-mRNA-1

Gene Sequence

>Msa0031120

ATGGCCAGTCATAACAACTGGTTCTTGGTTTCTTCTCTCTTGCTTACATTTCTCCAAATCCAAACCAAAGTGTTTTGCTACCAATTCAAAGTTGGAGATCTAAATGCTTGGGGTATACCCACCTCAGCAAATCCACAAGTCTATGCTAAATGGTCCAAATTTCACAACTTCACCCTTGGAGACTCACTTTTGTTTCTATATCCACCAAGTCAAGATTCGTTGATTCAAGTAACACAAGAATCTTACAAGAGCTGCAACACTAAAGATCCAATATTGTTTATGAACAATGGTAACTCATTGTTTAACATAACATCACATGGTGATTTCTACTTCACAAGTGGAGAAAATGGTCATTGCCAAAAGAATCAAAAGATTCATATATCTGTTGGAGGAATTGGAAATGTTGATGCTGAAGCTAATGGTCCAAGTTCTTCATTGCCTGCATCTGCACCCTCTTCACAAACAGTGTTTGGAAACATTCCAGTAGCTCCTTCTTCATCAAATTCACCTCACCTAACTTCAACTTTTCATGTGTTTATTATTGGATCTCTCATATATGCCTATTTTTTACTTTAA

Protein sequence

>Msa0031120

MASHNNWFLVSSLLLTFLQIQTKVFCYQFKVGDLNAWGIPTSANPQVYAKWSKFHNFTLGDSLLFLYPPSQDSLIQVTQESYKSCNTKDPILFMNNGNSLFNITSHGDFYFTSGENGHCQKNQKIHISVGGIGNVDAEANGPSSSLPASAPSSQTVFGNIPVAPSSSNSPHLTSTFHVFIIGSLIYAYFLL*