AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0086770

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0086770 MtrunA17_Chr4g0008081 96.524 374 6 2 1 374 66 432 0.0 735
Msa0086770 MtrunA17_Chr1g0202981 98.880 357 4 0 18 374 1 357 0.0 727
Msa0086770 MtrunA17_Chr4g0062991 49.032 155 70 3 200 348 2 153 1.35e-40 141
Msa0086770 MtrunA17_Chr3g0104471 23.569 297 211 6 44 330 106 396 4.41e-12 67.0
Msa0086770 MtrunA17_Chr3g0130221 23.145 337 227 10 22 344 24 342 4.77e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0086770 AT1G76340.1 80.347 346 65 2 30 372 25 370 0.0 546
Msa0086770 AT5G19980.1 49.091 330 161 3 36 358 10 339 3.79e-101 303
Msa0086770 AT2G13650.3 24.333 300 213 5 46 336 39 333 2.24e-12 67.8
Msa0086770 AT2G13650.1 24.333 300 213 5 46 336 39 333 2.24e-12 67.8

Find 88 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATGATTAATTTGGTGATA+TGG 0.203404 1_2:-67505783 Msa0086770:CDS
GTTCATCAAATTTCAGTATA+TGG 0.213733 1_2:-67506573 Msa0086770:CDS
CATGTGGGCGGTGGCGTATT+TGG 0.213873 1_2:-67506121 Msa0086770:CDS
TCCCCTACAGCAACGAAAAT+TGG 0.244454 1_2:+67506261 None:intergenic
TATCATTGGTAAACCAATAT+TGG 0.256096 1_2:+67505454 None:intergenic
CACAACAGTCAATCCAATAT+TGG 0.268433 1_2:-67505467 Msa0086770:three_prime_UTR
GGTCTGGCGATCTCTTTCTT+TGG 0.299461 1_2:-67505877 Msa0086770:CDS
TGCTGTCAATTATCAACAAA+TGG 0.310945 1_2:-67506518 Msa0086770:CDS
TCCGCTGGAATTGTTGATAA+TGG 0.331742 1_2:-67505992 Msa0086770:CDS
CGCAGGGCTATTTCTGCAAC+TGG 0.345581 1_2:-67505844 Msa0086770:CDS
GTGGCGTTGTCGTGCTTGTT+TGG 0.347148 1_2:-67505898 Msa0086770:CDS
GTTGACGGTTATGATTAATT+TGG 0.350016 1_2:-67505791 Msa0086770:CDS
TATGATTAATTTGGTGATAT+GGG 0.359061 1_2:-67505782 Msa0086770:CDS
GCAACTGGATTTACTGTTCT+CGG 0.370937 1_2:-67505829 Msa0086770:CDS
CCGCTGGAATTGTTGATAAT+GGG 0.392791 1_2:-67505991 Msa0086770:CDS
AAATTCTTCACGTTACTAGT+AGG 0.398839 1_2:+67506651 None:intergenic
AATGAGAATGATGGTGTTGC+TGG 0.408683 1_2:-67506615 Msa0086770:CDS
TTTGAAGACGTGGGCTTCGT+TGG 0.410068 1_2:-67506208 Msa0086770:CDS
TTGAAGACGTGGGCTTCGTT+GGG 0.412050 1_2:-67506207 Msa0086770:CDS
TAAACCGAAGGCTGCGATAC+AGG 0.412609 1_2:-67505680 Msa0086770:CDS
GTTTCCTGTATCGCAGCCTT+CGG 0.449049 1_2:+67505676 None:intergenic
GCTGTCAATTATCAACAAAT+GGG 0.454438 1_2:-67506517 Msa0086770:CDS
ATGATGGTGTTGCTGGAAAC+TGG 0.466449 1_2:-67506608 Msa0086770:CDS
CAACGATTGAATTGAACACT+TGG 0.468132 1_2:-67506056 Msa0086770:CDS
AATTTCAGTATCACTAATGT+TGG 0.470014 1_2:+67505597 None:intergenic
GTAACGTAATCCGAACATAC+TGG 0.470095 1_2:+67505327 None:intergenic
GTTGTCGTGCTTGTTTGGTC+TGG 0.470531 1_2:-67505893 Msa0086770:CDS
ATACTGAAATTAATAAATCA+AGG 0.471333 1_2:-67505585 Msa0086770:CDS
TGCAGTTCTCAGTCTCTCTC+TGG 0.474638 1_2:-67508505 Msa0086770:five_prime_UTR
AATTCTTCACGTTACTAGTA+GGG 0.474774 1_2:+67506652 None:intergenic
CACTGTTGGTAAACAGTGAC+AGG 0.483050 1_2:+67506337 None:intergenic
TACTTTCATTGTGTTCCGAT+CGG 0.492664 1_2:-67506289 Msa0086770:CDS
TCAGTATATGGTGTTGCTGC+TGG 0.493201 1_2:-67506561 Msa0086770:CDS
GACATTCACTGAGATATCAT+TGG 0.499001 1_2:+67505440 None:intergenic
AACGCCACAGGCAAAACCAC+AGG 0.500816 1_2:+67505913 None:intergenic
AACAAGCACGACAACGCCAC+AGG 0.501250 1_2:+67505901 None:intergenic
TGCGGCTTACATGTGGGCGG+TGG 0.503669 1_2:-67506130 Msa0086770:CDS
TGTAACGCGGTAAGCGCACC+CGG 0.509471 1_2:+67506474 None:intergenic
CAGTGTTTCTGCATCGGCCG+TGG 0.509990 1_2:-67506236 Msa0086770:CDS
TTTGATTTGTATGCTAGGTG+GGG 0.511236 1_2:-67505725 Msa0086770:CDS
TCGTTGGGAACTATATTCGC+TGG 0.512357 1_2:-67506192 Msa0086770:CDS
GAGACTGGTGAGAAGAGGAC+TGG 0.514293 1_2:+67508556 None:intergenic
TGTCATGAAATTTCCTTACC+CGG 0.516107 1_2:-67506493 Msa0086770:CDS
TATAGTGAATAAGTTGTTGA+CGG 0.516373 1_2:-67505806 Msa0086770:CDS
AGAGAGAGTGAGCAGATTGG+TGG 0.517600 1_2:+67508472 None:intergenic
AACGATTGAATTGAACACTT+GGG 0.519198 1_2:-67506055 Msa0086770:CDS
TAGCAAAACTCCGGCAGCAC+TGG 0.521554 1_2:+67506443 None:intergenic
CAGTACTTCACCAGTGCTGC+CGG 0.521678 1_2:-67506453 Msa0086770:CDS
CGTGGCCGTCTTTGAAGACG+TGG 0.524674 1_2:-67506218 Msa0086770:CDS
AAGAGAGAGAGTGAGCAGAT+TGG 0.527889 1_2:+67508469 None:intergenic
GCGGTAAGCGCACCCGGGTA+AGG 0.528937 1_2:+67506480 None:intergenic
GAGAGTGAGCAGATTGGTGG+AGG 0.535238 1_2:+67508475 None:intergenic
AGTCGCGATAAGAGTGAGAC+TGG 0.542135 1_2:+67508541 None:intergenic
TCGACGTGGGTGGGTACAGT+GGG 0.543028 1_2:-67505751 Msa0086770:CDS
TTTGCTATGTGGATGGCTCA+AGG 0.548441 1_2:-67506427 Msa0086770:CDS
GTTCTCACTCACTCAAACTC+AGG 0.548486 1_2:-67508411 Msa0086770:intron
AGATCACTGATGAGTCTGAT+TGG 0.551515 1_2:-67505945 Msa0086770:CDS
AGAGTGAGACTGGTGAGAAG+AGG 0.554192 1_2:+67508551 None:intergenic
CGAAAATTGGAACAGCCGAT+CGG 0.559850 1_2:+67506274 None:intergenic
GGGAGTCAGTGTTTCTGCAT+CGG 0.560622 1_2:-67506242 Msa0086770:CDS
CTCGACGTGGGTGGGTACAG+TGG 0.569198 1_2:-67505752 Msa0086770:CDS
AGACTGGTGAGAAGAGGACT+GGG 0.571748 1_2:+67508557 None:intergenic
GTCATGAAATTTCCTTACCC+GGG 0.580675 1_2:-67506492 Msa0086770:CDS
TGTGTTGTACAATAATATTG+AGG 0.586598 1_2:-67506028 Msa0086770:CDS
TCAGTGAATGTCATGCTCAT+CGG 0.587493 1_2:-67505429 Msa0086770:three_prime_UTR
GAAATTAATAAATCAAGGGA+AGG 0.592364 1_2:-67505580 Msa0086770:CDS
ACGATTGAATTGAACACTTG+GGG 0.594882 1_2:-67506054 Msa0086770:CDS
GAAGCCCACGTCTTCAAAGA+CGG 0.608352 1_2:+67506213 None:intergenic
ATGAATGTGAATGAGAATGA+TGG 0.610284 1_2:-67506624 Msa0086770:CDS
CACGTCTTCAAAGACGGCCA+CGG 0.612914 1_2:+67506219 None:intergenic
GGATAAGCACTCGACGTGGG+TGG 0.617289 1_2:-67505761 Msa0086770:CDS
GATAAGCACTCGACGTGGGT+GGG 0.619780 1_2:-67505760 Msa0086770:CDS
GGAAACTACACAAGAAGATG+AGG 0.619840 1_2:-67505659 Msa0086770:CDS
CCATCCACATAGCAAAACTC+CGG 0.620293 1_2:+67506434 None:intergenic
TACTGAAATTAATAAATCAA+GGG 0.623472 1_2:-67505584 Msa0086770:CDS
CACAATGAAAGTATCTACGT+TGG 0.624331 1_2:+67506299 None:intergenic
GTGGCCGTCTTTGAAGACGT+GGG 0.627250 1_2:-67506217 Msa0086770:CDS
TGATTGGCATTCATTCCCTG+TGG 0.634911 1_2:-67505929 Msa0086770:CDS
ATGTAACAGTAACTCACTGT+TGG 0.640519 1_2:+67506323 None:intergenic
GCAATCAACAAGTAAACCGA+AGG 0.642301 1_2:-67505692 Msa0086770:CDS
ACGCCACAGGCAAAACCACA+GGG 0.653659 1_2:+67505914 None:intergenic
CCCATTATCAACAATTCCAG+CGG 0.654894 1_2:+67505991 None:intergenic
TTGATTTGATGACAATGTGG+AGG 0.666318 1_2:-67506386 Msa0086770:CDS
ATGGGATAAGCACTCGACGT+GGG 0.666646 1_2:-67505764 Msa0086770:CDS
TATGGGATAAGCACTCGACG+TGG 0.700984 1_2:-67505765 Msa0086770:CDS
AGCTTGATTTGATGACAATG+TGG 0.707635 1_2:-67506389 Msa0086770:CDS
GTAACGCGGTAAGCGCACCC+GGG 0.720177 1_2:+67506475 None:intergenic
ACTGGTGAAGTACTGTAACG+CGG 0.793914 1_2:+67506461 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAGGTTTTTTTTTTTAATT+TGG - chr1_2:67506038-67506057 Msa0086770:CDS 10.0%
!!! TAGGTTTTTTTTTTTAATTT+GGG - chr1_2:67506039-67506058 Msa0086770:CDS 10.0%
!! TAAAGAATATCATTAAAAAA+AGG + chr1_2:67506290-67506309 None:intergenic 10.0%
!!! TTTTATATTTTTTTACTTGT+TGG - chr1_2:67506614-67506633 Msa0086770:CDS 10.0%
!!! AATTTTGTTATGATTAGAAT+TGG - chr1_2:67505594-67505613 Msa0086770:CDS 15.0%
!!! AGGTTTTTTTTTTTAATTTG+GGG - chr1_2:67506040-67506059 Msa0086770:CDS 15.0%
!! GAAAATTTATTAAAATACAC+CGG - chr1_2:67506479-67506498 Msa0086770:CDS 15.0%
!!! ATTTATTTGAGTTTATAGTA+AGG + chr1_2:67506545-67506564 None:intergenic 15.0%
!!! AGATTTTAGATTGATATTAA+TGG - chr1_2:67507194-67507213 Msa0086770:intron 15.0%
!! ATACTGAAATTAATAAATCA+AGG - chr1_2:67508331-67508350 Msa0086770:intron 15.0%
!! TACTGAAATTAATAAATCAA+GGG - chr1_2:67508332-67508351 Msa0086770:intron 15.0%
!! AAAAATGATATAATTTGGAT+TGG + chr1_2:67508428-67508447 None:intergenic 15.0%
!! TGTGAAAAAATGATATAATT+TGG + chr1_2:67508433-67508452 None:intergenic 15.0%
!!! TACATTTTCTAAACTGTATT+TGG + chr1_2:67505835-67505854 None:intergenic 20.0%
!! TAAAATACTATGTATTGCAT+TGG - chr1_2:67505888-67505907 Msa0086770:CDS 20.0%
!!! GTTTCAATTTCTCATTTTAT+AGG - chr1_2:67506260-67506279 Msa0086770:CDS 20.0%
!!! TTTTTTTACTTGTTGGTTAT+AGG - chr1_2:67506621-67506640 Msa0086770:CDS 20.0%
!!! AGAAAATTTTCATGTTCAAT+AGG + chr1_2:67506681-67506700 None:intergenic 20.0%
!! AATAGTATTATGACATTCAT+TGG - chr1_2:67506726-67506745 Msa0086770:intron 20.0%
!!! TTATGATTAATTTGGTGATA+TGG - chr1_2:67508133-67508152 Msa0086770:intron 20.0%
!!! TATGATTAATTTGGTGATAT+GGG - chr1_2:67508134-67508153 Msa0086770:intron 20.0%
!! AAAAATTATTCCAGTATGTT+CGG - chr1_2:67508579-67508598 Msa0086770:five_prime_UTR 20.0%
! TTTGTATACTATTGTTCTCT+TGG + chr1_2:67505686-67505705 None:intergenic 25.0%
! AACTAATCTAGATATCTCTT+TGG + chr1_2:67505802-67505821 None:intergenic 25.0%
! GTATGCAATATTGTTTATAC+AGG + chr1_2:67505946-67505965 None:intergenic 25.0%
!! GAAAATCACTTAGGAATTTT+AGG - chr1_2:67506020-67506039 Msa0086770:CDS 25.0%
!! TTTGTTGTATTCTTCGAAAT+TGG - chr1_2:67506065-67506084 Msa0086770:CDS 25.0%
! TTGTTGTATTCTTCGAAATT+GGG - chr1_2:67506066-67506085 Msa0086770:CDS 25.0%
! ATGAGGAATCTTAAAAAACT+TGG + chr1_2:67506194-67506213 None:intergenic 25.0%
! AACAAAAAAGAATACCTCTA+TGG + chr1_2:67506243-67506262 None:intergenic 25.0%
!! TTGTAAAGCTTATGATTTTC+CGG + chr1_2:67506501-67506520 None:intergenic 25.0%
!!! TAAGAACTTTGAAATTGCAT+TGG + chr1_2:67506583-67506602 None:intergenic 25.0%
! TTCAAAGTTCTTATCTTTGA+AGG - chr1_2:67506590-67506609 Msa0086770:CDS 25.0%
! TTTCAAGCATAAATTAAGTC+CGG + chr1_2:67507016-67507035 None:intergenic 25.0%
! GTTCATCAAATTTCAGTATA+TGG - chr1_2:67507343-67507362 Msa0086770:intron 25.0%
!!! TGATTATCAGTTTACTTTTG+CGG - chr1_2:67507768-67507787 Msa0086770:intron 25.0%
!!! TTTTGTTTACATAAAGCATG+TGG - chr1_2:67507834-67507853 Msa0086770:intron 25.0%
! TGTGTTGTACAATAATATTG+AGG - chr1_2:67507888-67507907 Msa0086770:intron 25.0%
! TATAGTGAATAAGTTGTTGA+CGG - chr1_2:67508110-67508129 Msa0086770:intron 25.0%
! AATTTCAGTATCACTAATGT+TGG + chr1_2:67508322-67508341 None:intergenic 25.0%
! GAAATTAATAAATCAAGGGA+AGG - chr1_2:67508336-67508355 Msa0086770:intron 25.0%
!! CTGTGAAAAATATACTTTTG+AGG + chr1_2:67508401-67508420 None:intergenic 25.0%
!!! CTCAAAAGTATATTTTTCAC+AGG - chr1_2:67508399-67508418 Msa0086770:intron 25.0%
! TATCATTGGTAAACCAATAT+TGG + chr1_2:67508465-67508484 None:intergenic 25.0%
!!! GCATTTTGTTTACGTTATAT+TGG - chr1_2:67508523-67508542 Msa0086770:five_prime_UTR 25.0%
!!! CTGTGTTTTTTACAGAATCA+GGG + chr1_2:67505335-67505354 None:intergenic 30.0%
!!! TCTGTGTTTTTTACAGAATC+AGG + chr1_2:67505336-67505355 None:intergenic 30.0%
TTAATTCCTTGCAGAAATGA+AGG + chr1_2:67505859-67505878 None:intergenic 30.0%
TGCAATATTGTTTATACAGG+TGG + chr1_2:67505943-67505962 None:intergenic 30.0%
ACAGAAAGAGAAAATCACTT+AGG - chr1_2:67506011-67506030 Msa0086770:CDS 30.0%
! TCGAAATTGGGAATTTTTGT+TGG - chr1_2:67506078-67506097 Msa0086770:CDS 30.0%
!!! GATAAATGACTTTTCAAGTC+TGG + chr1_2:67506147-67506166 None:intergenic 30.0%
!! AAAATTAGAGGTTCTAGATG+AGG + chr1_2:67506211-67506230 None:intergenic 30.0%
AAAAAAGAATACCTCTATGG+TGG + chr1_2:67506240-67506259 None:intergenic 30.0%
CTTTACAAACACTTACACAA+GGG - chr1_2:67506513-67506532 Msa0086770:CDS 30.0%
AGTATTATGACATTCATTGG+TGG - chr1_2:67506729-67506748 Msa0086770:intron 30.0%
!!! TTTTTTTTTTTGTGCGAGTG+TGG + chr1_2:67506865-67506884 None:intergenic 30.0%
ACTACAAGTTACAGTTCATA+TGG + chr1_2:67506991-67507010 None:intergenic 30.0%
TTCACCTTAAAGAATCTAAG+CGG + chr1_2:67507161-67507180 None:intergenic 30.0%
AATTCTTCACGTTACTAGTA+GGG + chr1_2:67507267-67507286 None:intergenic 30.0%
AAATTCTTCACGTTACTAGT+AGG + chr1_2:67507268-67507287 None:intergenic 30.0%
ATGAATGTGAATGAGAATGA+TGG - chr1_2:67507292-67507311 Msa0086770:intron 30.0%
TGCTGTCAATTATCAACAAA+TGG - chr1_2:67507398-67507417 Msa0086770:intron 30.0%
GCTGTCAATTATCAACAAAT+GGG - chr1_2:67507399-67507418 Msa0086770:intron 30.0%
AACGATTGAATTGAACACTT+GGG - chr1_2:67507861-67507880 Msa0086770:intron 30.0%
GTTGACGGTTATGATTAATT+TGG - chr1_2:67508125-67508144 Msa0086770:intron 30.0%
!!! TTTTGATTTGTATGCTAGGT+GGG - chr1_2:67508190-67508209 Msa0086770:intron 30.0%
!! AGTCTCCAATTGCTTGTTTT+AGG - chr1_2:67505539-67505558 Msa0086770:three_prime_UTR 35.0%
!!! GTCTCCAATTGCTTGTTTTA+GGG - chr1_2:67505540-67505559 Msa0086770:three_prime_UTR 35.0%
GAAACCCTAAAACAAGCAAT+TGG + chr1_2:67505547-67505566 None:intergenic 35.0%
!!! TTGTTTTAGGGTTTCCGAAT+CGG - chr1_2:67505552-67505571 Msa0086770:three_prime_UTR 35.0%
TCCAATTCTAATTCCCGATT+CGG + chr1_2:67505569-67505588 None:intergenic 35.0%
GCTATTAGAAAGATTTCTCG+TGG + chr1_2:67505746-67505765 None:intergenic 35.0%
! AAAAAACTTGGCTCGCAAAA+AGG + chr1_2:67506182-67506201 None:intergenic 35.0%
! TGGTGGATCTGTAAAATTAG+AGG + chr1_2:67506223-67506242 None:intergenic 35.0%
! ATTTTACAGATCCACCATAG+AGG - chr1_2:67506226-67506245 Msa0086770:CDS 35.0%
GCTTTACAAACACTTACACA+AGG - chr1_2:67506512-67506531 Msa0086770:CDS 35.0%
! TTTACTTGTTGGTTATAGGC+AGG - chr1_2:67506625-67506644 Msa0086770:CDS 35.0%
CAGTCAAAGTCAAAGATGAA+TGG - chr1_2:67506702-67506721 Msa0086770:intron 35.0%
AGACTGCAGAGACATTTAAT+TGG + chr1_2:67506964-67506983 None:intergenic 35.0%
! GAACTGTAACTTGTAGTTAC+CGG - chr1_2:67506994-67507013 Msa0086770:intron 35.0%
TGCTTGAAAGAGATGATGTA+TGG - chr1_2:67507027-67507046 Msa0086770:intron 35.0%
TGAGTTGTACTTACAAGACT+CGG + chr1_2:67507058-67507077 None:intergenic 35.0%
TGTCATGAAATTTCCTTACC+CGG - chr1_2:67507423-67507442 Msa0086770:intron 35.0%
! AGCTTGATTTGATGACAATG+TGG - chr1_2:67507527-67507546 Msa0086770:intron 35.0%
! TTGATTTGATGACAATGTGG+AGG - chr1_2:67507530-67507549 Msa0086770:intron 35.0%
ATGTAACAGTAACTCACTGT+TGG + chr1_2:67507596-67507615 None:intergenic 35.0%
!! CACAATGAAAGTATCTACGT+TGG + chr1_2:67507620-67507639 None:intergenic 35.0%
TACTTTCATTGTGTTCCGAT+CGG - chr1_2:67507627-67507646 Msa0086770:intron 35.0%
! TTCCAATTTTCGTTGCTGTA+GGG - chr1_2:67507653-67507672 Msa0086770:intron 35.0%
CAACGATTGAATTGAACACT+TGG - chr1_2:67507860-67507879 Msa0086770:intron 35.0%
ACGATTGAATTGAACACTTG+GGG - chr1_2:67507862-67507881 Msa0086770:intron 35.0%
!!! GGACTTTTGATTTGTATGCT+AGG - chr1_2:67508186-67508205 Msa0086770:intron 35.0%
!!! CTTTTGATTTGTATGCTAGG+TGG - chr1_2:67508189-67508208 Msa0086770:intron 35.0%
!! TTTGATTTGTATGCTAGGTG+GGG - chr1_2:67508191-67508210 Msa0086770:intron 35.0%
CACAACAGTCAATCCAATAT+TGG - chr1_2:67508449-67508468 Msa0086770:five_prime_UTR 35.0%
GACATTCACTGAGATATCAT+TGG + chr1_2:67508479-67508498 None:intergenic 35.0%
CAAGCAATTGGAGACTGAAT+TGG + chr1_2:67505535-67505554 None:intergenic 40.0%
!!! TGTTTTAGGGTTTCCGAATC+GGG - chr1_2:67505553-67505572 Msa0086770:three_prime_UTR 40.0%
TCCGAATCGGGAATTAGAAT+TGG - chr1_2:67505565-67505584 Msa0086770:exon 40.0%
! ACTATTGTTCTCTTGGCTCT+GGG + chr1_2:67505679-67505698 None:intergenic 40.0%
TACTATTGTTCTCTTGGCTC+TGG + chr1_2:67505680-67505699 None:intergenic 40.0%
ATGTAGCCTTCATTTCTGCA+AGG - chr1_2:67505850-67505869 Msa0086770:CDS 40.0%
TCCATAGACCTCTTCACTAA+TGG - chr1_2:67505974-67505993 Msa0086770:CDS 40.0%
AAGCAATGCCATTAGTGAAG+AGG + chr1_2:67505985-67506004 None:intergenic 40.0%
!!! GACTTTTCAAGTCTGGCAAA+TGG + chr1_2:67506140-67506159 None:intergenic 40.0%
!!! GGTTTTTTTAGCGCGTTGAT+GGG + chr1_2:67506660-67506679 None:intergenic 40.0%
!!! AGGTTTTTTTAGCGCGTTGA+TGG + chr1_2:67506661-67506680 None:intergenic 40.0%
ATGAGCGCATTACTTGTCAT+AGG + chr1_2:67506784-67506803 None:intergenic 40.0%
AATGCGCTCATTTGATATGC+TGG - chr1_2:67506793-67506812 Msa0086770:intron 40.0%
ATGCGCTCATTTGATATGCT+GGG - chr1_2:67506794-67506813 Msa0086770:intron 40.0%
TAAAGCAGAGTGTGAGAAGT+GGG + chr1_2:67506835-67506854 None:intergenic 40.0%
TCTGAGAAGTGAGGAATTGT+GGG - chr1_2:67506920-67506939 Msa0086770:intron 40.0%
!! ACATTTAATTGGCTCGAGAG+CGG + chr1_2:67506953-67506972 None:intergenic 40.0%
AACTATGAATTCACACCCCA+GGG + chr1_2:67507111-67507130 None:intergenic 40.0%
ATTACACACACTGAGAAGTC+AGG + chr1_2:67507134-67507153 None:intergenic 40.0%
TTCGCCGCTTAGATTCTTTA+AGG - chr1_2:67507154-67507173 Msa0086770:intron 40.0%
!! AATGAGAATGATGGTGTTGC+TGG - chr1_2:67507301-67507320 Msa0086770:intron 40.0%
GTCATGAAATTTCCTTACCC+GGG - chr1_2:67507424-67507443 Msa0086770:intron 40.0%
AGGTAAAAAATCACAGCAGC+CGG + chr1_2:67507562-67507581 None:intergenic 40.0%
! GTTCCAATTTTCGTTGCTGT+AGG - chr1_2:67507652-67507671 Msa0086770:intron 40.0%
! TCCAATTTTCGTTGCTGTAG+GGG - chr1_2:67507654-67507673 Msa0086770:intron 40.0%
!! TTACTTTTGCGGCTTACATG+TGG - chr1_2:67507779-67507798 Msa0086770:intron 40.0%
! TACTTTTGCGGCTTACATGT+GGG - chr1_2:67507780-67507799 Msa0086770:intron 40.0%
! TCCGCTGGAATTGTTGATAA+TGG - chr1_2:67507924-67507943 Msa0086770:intron 40.0%
CCCATTATCAACAATTCCAG+CGG + chr1_2:67507928-67507947 None:intergenic 40.0%
!! CCGCTGGAATTGTTGATAAT+GGG - chr1_2:67507925-67507944 Msa0086770:intron 40.0%
AGATCACTGATGAGTCTGAT+TGG - chr1_2:67507971-67507990 Msa0086770:intron 40.0%
GCAACTGGATTTACTGTTCT+CGG - chr1_2:67508087-67508106 Msa0086770:intron 40.0%
GCAATCAACAAGTAAACCGA+AGG - chr1_2:67508224-67508243 Msa0086770:intron 40.0%
GGAAACTACACAAGAAGATG+AGG - chr1_2:67508257-67508276 Msa0086770:intron 40.0%
TCAGTGAATGTCATGCTCAT+CGG - chr1_2:67508487-67508506 Msa0086770:five_prime_UTR 40.0%
GTAACGTAATCCGAACATAC+TGG + chr1_2:67508592-67508611 None:intergenic 40.0%
AAGAGAGAGAGTGAGCAGAT+TGG + chr1_2:67505450-67505469 None:intergenic 45.0%
GTTCTCACTCACTCAAACTC+AGG - chr1_2:67505505-67505524 Msa0086770:three_prime_UTR 45.0%
GCCATTAGTGAAGAGGTCTA+TGG + chr1_2:67505978-67505997 None:intergenic 45.0%
AAAGCAGAGTGTGAGAAGTG+GGG + chr1_2:67506834-67506853 None:intergenic 45.0%
CTAAAGCAGAGTGTGAGAAG+TGG + chr1_2:67506836-67506855 None:intergenic 45.0%
! CTCTGAGAAGTGAGGAATTG+TGG - chr1_2:67506919-67506938 Msa0086770:intron 45.0%
!! CATTTAATTGGCTCGAGAGC+GGG + chr1_2:67506952-67506971 None:intergenic 45.0%
! CAGGGCATCAAATGTCTCTA+CGG + chr1_2:67507093-67507112 None:intergenic 45.0%
GTAGAGACATTTGATGCCCT+GGG - chr1_2:67507092-67507111 Msa0086770:intron 45.0%
TAGAGACATTTGATGCCCTG+GGG - chr1_2:67507093-67507112 Msa0086770:intron 45.0%
GAACTATGAATTCACACCCC+AGG + chr1_2:67507112-67507131 None:intergenic 45.0%
!! ATGATGGTGTTGCTGGAAAC+TGG - chr1_2:67507308-67507327 Msa0086770:intron 45.0%
!! TCAGTATATGGTGTTGCTGC+TGG - chr1_2:67507355-67507374 Msa0086770:intron 45.0%
!! ACTGGTGAAGTACTGTAACG+CGG + chr1_2:67507458-67507477 None:intergenic 45.0%
CCATCCACATAGCAAAACTC+CGG + chr1_2:67507485-67507504 None:intergenic 45.0%
! TTTGCTATGTGGATGGCTCA+AGG - chr1_2:67507489-67507508 Msa0086770:intron 45.0%
! GACAATGTGGAGGTTTTTGC+CGG - chr1_2:67507540-67507559 Msa0086770:intron 45.0%
! CACTGTTGGTAAACAGTGAC+AGG + chr1_2:67507582-67507601 None:intergenic 45.0%
CGAAAATTGGAACAGCCGAT+CGG + chr1_2:67507645-67507664 None:intergenic 45.0%
TCCCCTACAGCAACGAAAAT+TGG + chr1_2:67507658-67507677 None:intergenic 45.0%
TCGTTGGGAACTATATTCGC+TGG - chr1_2:67507724-67507743 Msa0086770:intron 45.0%
TGATTGGCATTCATTCCCTG+TGG - chr1_2:67507987-67508006 Msa0086770:intron 45.0%
! TCTTTGGATTTTCGTGTCGC+AGG - chr1_2:67508055-67508074 Msa0086770:intron 45.0%
! CTTTGGATTTTCGTGTCGCA+GGG - chr1_2:67508056-67508075 Msa0086770:intron 45.0%
AGACTGGTGAGAAGAGGACT+GGG + chr1_2:67505362-67505381 None:intergenic 50.0%
AGAGTGAGACTGGTGAGAAG+AGG + chr1_2:67505368-67505387 None:intergenic 50.0%
AGTCGCGATAAGAGTGAGAC+TGG + chr1_2:67505378-67505397 None:intergenic 50.0%
TGCAGTTCTCAGTCTCTCTC+TGG - chr1_2:67505411-67505430 Msa0086770:three_prime_UTR 50.0%
AGAGAGAGTGAGCAGATTGG+TGG + chr1_2:67505447-67505466 None:intergenic 50.0%
! CACACACACTCTGAGAAGTG+AGG - chr1_2:67506911-67506930 Msa0086770:intron 50.0%
CGTAGAGACATTTGATGCCC+TGG - chr1_2:67507091-67507110 Msa0086770:intron 50.0%
!! CCGGAGTTTTGCTATGTGGA+TGG - chr1_2:67507482-67507501 Msa0086770:intron 50.0%
! GGGAGTCAGTGTTTCTGCAT+CGG - chr1_2:67507674-67507693 Msa0086770:intron 50.0%
GAAGCCCACGTCTTCAAAGA+CGG + chr1_2:67507706-67507725 None:intergenic 50.0%
TTTGAAGACGTGGGCTTCGT+TGG - chr1_2:67507708-67507727 Msa0086770:intron 50.0%
TTGAAGACGTGGGCTTCGTT+GGG - chr1_2:67507709-67507728 Msa0086770:intron 50.0%
! TTTTGCGGCTTACATGTGGG+CGG - chr1_2:67507783-67507802 Msa0086770:intron 50.0%
!! ATTCCCTGTGGTTTTGCCTG+TGG - chr1_2:67507999-67508018 Msa0086770:intron 50.0%
GTTGTCGTGCTTGTTTGGTC+TGG - chr1_2:67508023-67508042 Msa0086770:intron 50.0%
GGTCTGGCGATCTCTTTCTT+TGG - chr1_2:67508039-67508058 Msa0086770:intron 50.0%
TATGGGATAAGCACTCGACG+TGG - chr1_2:67508151-67508170 Msa0086770:intron 50.0%
ATGGGATAAGCACTCGACGT+GGG - chr1_2:67508152-67508171 Msa0086770:intron 50.0%
TAAACCGAAGGCTGCGATAC+AGG - chr1_2:67508236-67508255 Msa0086770:intron 50.0%
GTTTCCTGTATCGCAGCCTT+CGG + chr1_2:67508243-67508262 None:intergenic 50.0%
GAGACTGGTGAGAAGAGGAC+TGG + chr1_2:67505363-67505382 None:intergenic 55.0%
GAGAGTGAGCAGATTGGTGG+AGG + chr1_2:67505444-67505463 None:intergenic 55.0%
CAGTACTTCACCAGTGCTGC+CGG - chr1_2:67507463-67507482 Msa0086770:intron 55.0%
TAGCAAAACTCCGGCAGCAC+TGG + chr1_2:67507476-67507495 None:intergenic 55.0%
!! GCTGCCGGAGTTTTGCTATG+TGG - chr1_2:67507478-67507497 Msa0086770:intron 55.0%
CACGTCTTCAAAGACGGCCA+CGG + chr1_2:67507700-67507719 None:intergenic 55.0%
! GTGGCCGTCTTTGAAGACGT+GGG - chr1_2:67507699-67507718 Msa0086770:intron 55.0%
!!! GGCTCTTCTGCTTTTTCCGC+TGG - chr1_2:67507909-67507928 Msa0086770:intron 55.0%
ACGCCACAGGCAAAACCACA+GGG + chr1_2:67508005-67508024 None:intergenic 55.0%
AACGCCACAGGCAAAACCAC+AGG + chr1_2:67508006-67508025 None:intergenic 55.0%
AACAAGCACGACAACGCCAC+AGG + chr1_2:67508018-67508037 None:intergenic 55.0%
GTGGCGTTGTCGTGCTTGTT+TGG - chr1_2:67508018-67508037 Msa0086770:intron 55.0%
!! CGCAGGGCTATTTCTGCAAC+TGG - chr1_2:67508072-67508091 Msa0086770:intron 55.0%
GATAAGCACTCGACGTGGGT+GGG - chr1_2:67508156-67508175 Msa0086770:intron 55.0%
TGTAACGCGGTAAGCGCACC+CGG + chr1_2:67507445-67507464 None:intergenic 60.0%
! CAGTGTTTCTGCATCGGCCG+TGG - chr1_2:67507680-67507699 Msa0086770:intron 60.0%
! CGTGGCCGTCTTTGAAGACG+TGG - chr1_2:67507698-67507717 Msa0086770:intron 60.0%
CATGTGGGCGGTGGCGTATT+TGG - chr1_2:67507795-67507814 Msa0086770:intron 60.0%
GGATAAGCACTCGACGTGGG+TGG - chr1_2:67508155-67508174 Msa0086770:intron 60.0%
TCGACGTGGGTGGGTACAGT+GGG - chr1_2:67508165-67508184 Msa0086770:intron 60.0%
GTAACGCGGTAAGCGCACCC+GGG + chr1_2:67507444-67507463 None:intergenic 65.0%
TGCGGCTTACATGTGGGCGG+TGG - chr1_2:67507786-67507805 Msa0086770:intron 65.0%
CTCGACGTGGGTGGGTACAG+TGG - chr1_2:67508164-67508183 Msa0086770:intron 65.0%
GCGGTAAGCGCACCCGGGTA+AGG + chr1_2:67507439-67507458 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 67505307 67508631 67505307 ID=Msa0086770;Name=Msa0086770
chr1_2 mRNA 67505307 67508631 67505307 ID=Msa0086770-mRNA-1;Parent=Msa0086770;Name=Msa0086770-mRNA-1;_AED=0.00;_eAED=0.00;_QI=220|1|1|1|0|0.5|2|266|374
chr1_2 exon 67505307 67506697 67505307 ID=Msa0086770-mRNA-1:exon:22947;Parent=Msa0086770-mRNA-1
chr1_2 exon 67508412 67508631 67508412 ID=Msa0086770-mRNA-1:exon:22946;Parent=Msa0086770-mRNA-1
chr1_2 five_prime_UTR 67508412 67508631 67508412 ID=Msa0086770-mRNA-1:five_prime_utr;Parent=Msa0086770-mRNA-1
chr1_2 CDS 67505573 67506697 67505573 ID=Msa0086770-mRNA-1:cds;Parent=Msa0086770-mRNA-1
chr1_2 three_prime_UTR 67505307 67505572 67505307 ID=Msa0086770-mRNA-1:three_prime_utr;Parent=Msa0086770-mRNA-1
Gene Sequence

>Msa0086770

ATGTCAAAAGTTGTTGAAAAAAACCCTACTAGTAACGTGAAGAATTTGAATATGAATGTGAATGAGAATGATGGTGTTGCTGGAAACTGGTACACTTCAATTGTTCATCAAATTTCAGTATATGGTGTTGCTGCTGGTTACTGTTTATCTGCATCTTTGCTGTCAATTATCAACAAATGGGCTGTCATGAAATTTCCTTACCCGGGTGCGCTTACCGCGTTACAGTACTTCACCAGTGCTGCCGGAGTTTTGCTATGTGGATGGCTCAAGGTTGTCGAACACGACAAGCTTGATTTGATGACAATGTGGAGGTTTTTGCCGGCTGCTGTGATTTTTTACCTGTCACTGTTTACCAACAGTGAGTTACTGTTACATGCCAACGTAGATACTTTCATTGTGTTCCGATCGGCTGTTCCAATTTTCGTTGCTGTAGGGGAGTCAGTGTTTCTGCATCGGCCGTGGCCGTCTTTGAAGACGTGGGCTTCGTTGGGAACTATATTCGCTGGAAGTGTGCTTTATGTTGCAACTGATTATCAGTTTACTTTTGCGGCTTACATGTGGGCGGTGGCGTATTTGGTTAGTATGACGATAGATTTTGTTTACATAAAGCATGTGGTTACAACGATTGAATTGAACACTTGGGGTCTTGTGTTGTACAATAATATTGAGGCTCTTCTGCTTTTTCCGCTGGAATTGTTGATAATGGGTGAGTTGAAGAAGATACAACATGAGATCACTGATGAGTCTGATTGGCATTCATTCCCTGTGGTTTTGCCTGTGGCGTTGTCGTGCTTGTTTGGTCTGGCGATCTCTTTCTTTGGATTTTCGTGTCGCAGGGCTATTTCTGCAACTGGATTTACTGTTCTCGGTATAGTGAATAAGTTGTTGACGGTTATGATTAATTTGGTGATATGGGATAAGCACTCGACGTGGGTGGGTACAGTGGGACTTTTGATTTGTATGCTAGGTGGGGTTATGTATCAGCAATCAACAAGTAAACCGAAGGCTGCGATACAGGAAACTACACAAGAAGATGAGGAACAACTGAAGTTGCTTGAAATGCAAGTCAATTCTGAGACCAACATTAGTGATACTGAAATTAATAAATCAAGGGAAGGAAATTGA
Protein sequence

>Msa0086770

MSKVVEKNPTSNVKNLNMNVNENDGVAGNWYTSIVHQISVYGVAAGYCLSASLLSIINKWAVMKFPYPGALTALQYFTSAAGVLLCGWLKVVEHDKLDLMTMWRFLPAAVIFYLSLFTNSELLLHANVDTFIVFRSAVPIFVAVGESVFLHRPWPSLKTWASLGTIFAGSVLYVATDYQFTFAAYMWAVAYLVSMTIDFVYIKHVVTTIELNTWGLVLYNNIEALLLFPLELLIMGELKKIQHEITDESDWHSFPVVLPVALSCLFGLAISFFGFSCRRAISATGFTVLGIVNKLLTVMINLVIWDKHSTWVGTVGLLICMLGGVMYQQSTSKPKAAIQETTQEDEEQLKLLEMQVNSETNISDTEINKSREGN*