AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0143170

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0143170 MtrunA17_Chr7g0266221 99.284 419 3 0 51 469 1 419 0.0 845
Msa0143170 MtrunA17_Chr1g0178681 71.360 419 120 0 51 469 1 419 0.0 589
Msa0143170 MtrunA17_Chr4g0026391 63.723 419 152 0 51 469 2 420 0.0 555
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0143170 AT1G67190.1 63.007 419 155 0 51 469 1 419 0.0 527
Msa0143170 AT1G67190.2 63.007 419 155 0 51 469 1 419 0.0 527

Find 103 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAACTTAAGGATTTAAAT+TGG 0.171218 1_4:-2100637 Msa0143170:three_prime_UTR
TTCTCAATGATATTGAAATT+AGG 0.193329 1_4:+2101810 None:intergenic
AATCATGAATTCAGTGAATT+TGG 0.195737 1_4:+2100966 None:intergenic
TCTCAATGATATTGAAATTA+GGG 0.201362 1_4:+2101811 None:intergenic
TACAATAAAATCTTTCATTA+AGG 0.228257 1_4:+2103742 None:intergenic
GAATCTGACATAGCGATTTC+TGG 0.230502 1_4:+2101603 None:intergenic
TAAGATCCTCCACAGTATTT+AGG 0.246972 1_4:+2100600 None:intergenic
TACCTTCTTGTCCGTCTTGC+CGG 0.277927 1_4:+2100841 None:intergenic
AGAGCAAATTTCGCGATATA+GGG 0.320402 1_4:+2102001 None:intergenic
AAGTTGCAATGGTGTTATAT+TGG 0.323507 1_4:+2100652 None:intergenic
CATCCTTAACTTGCAAGAAA+TGG 0.324453 1_4:-2102079 Msa0143170:CDS
AAAGATGGAGTGCTTCATTA+TGG 0.324477 1_4:-2101168 Msa0143170:CDS
TAGAGCAAATTTCGCGATAT+AGG 0.327459 1_4:+2102000 None:intergenic
GCAACTTAAGGATTTAAATT+GGG 0.331533 1_4:-2100636 Msa0143170:three_prime_UTR
AGTTTGGGTAGCTGGACTTT+TGG 0.334910 1_4:-2101070 Msa0143170:CDS
CTGTTACCCCTGTCATTGCT+TGG 0.349306 1_4:-2101887 Msa0143170:CDS
AATTCATAGGTACCATTAGA+TGG 0.353568 1_4:+2103840 None:intergenic
ATGTATTGAATGAAAGAGTT+TGG 0.353897 1_4:+2102033 None:intergenic
ATAGACAAGGTTTCAAGTTT+CGG 0.358073 1_4:+2101633 None:intergenic
TCTCCATTTCTTGCAAGTTA+AGG 0.360964 1_4:+2102076 None:intergenic
ATCTTTCAAGTCATAACTTA+AGG 0.368328 1_4:+2101185 None:intergenic
TATAACACCATTGCAACTTA+AGG 0.371601 1_4:-2100648 Msa0143170:three_prime_UTR
GCTTAGCAGCTCTTCTTTGA+AGG 0.394764 1_4:-2101565 Msa0143170:CDS
TTAAGTTATGACTTGAAAGA+TGG 0.408134 1_4:-2101183 Msa0143170:CDS
CCAATCTACATTGAATTCAT+AGG 0.409184 1_4:+2103827 None:intergenic
TAAATGAATCCTCCATCTAA+TGG 0.419177 1_4:-2103852 Msa0143170:CDS
AGGATCTTAATCAAACAGTT+TGG 0.423175 1_4:-2100586 Msa0143170:three_prime_UTR
CTTGGCTAGCAATCCTATCT+CGG 0.423380 1_4:-2101757 Msa0143170:CDS
TGTTAGGCGCTGCAATCCTT+TGG 0.433874 1_4:+2101938 None:intergenic
GTATCATATTATCCCATCTT+GGG 0.437104 1_4:-2102124 Msa0143170:CDS
GTGCTTCATTATGGCTTGCA+AGG 0.446222 1_4:-2101159 Msa0143170:CDS
CGAAATTTGCTCTAGTCACT+TGG 0.446849 1_4:-2101991 Msa0143170:CDS
ATGATACCGACCACTTCAAC+AGG 0.448381 1_4:+2102140 None:intergenic
AAGGAAAGGTGCGTTAATCT+AGG 0.450642 1_4:+2101204 None:intergenic
AGTATTGGTTTGATCTCATC+CGG 0.460308 1_4:-2100860 Msa0143170:three_prime_UTR
TACATAAGCCAAGCAATGAC+AGG 0.467063 1_4:+2101879 None:intergenic
TATCCCATCTTGGGTCTGCT+AGG 0.472254 1_4:-2102115 Msa0143170:CDS
TTGTAAATCAAATGCAATCA+AGG 0.474891 1_4:+2100558 None:intergenic
GGTATCATATTATCCCATCT+TGG 0.475174 1_4:-2102125 Msa0143170:CDS
ATCCCATCTTGGGTCTGCTA+GGG 0.476732 1_4:-2102114 Msa0143170:CDS
ATCTGTTGCTGTCAAAGTAT+TGG 0.476869 1_4:-2100875 Msa0143170:three_prime_UTR
CATCATCCATGAAAATTGTT+AGG 0.477751 1_4:+2101922 None:intergenic
TCTAGGAAAACAAACAGATA+TGG 0.480581 1_4:+2101221 None:intergenic
CAACTTAAGGATTTAAATTG+GGG 0.481345 1_4:-2100635 Msa0143170:three_prime_UTR
TTCGGCTCAACACCCGAGAT+AGG 0.481385 1_4:+2101744 None:intergenic
TTTAAATTGGGGTCGTGTTG+TGG 0.482965 1_4:-2100624 Msa0143170:three_prime_UTR
CATCCCTAGCAGACCCAAGA+TGG 0.487907 1_4:+2102111 None:intergenic
TTGAATATGAGTAGGAGGTT+CGG 0.495590 1_4:-2100906 Msa0143170:exon
CCTATGAATTCAATGTAGAT+TGG 0.496450 1_4:-2103827 Msa0143170:CDS
TAGATTGGTCTCGTATTAAG+AGG 0.502089 1_4:-2103812 Msa0143170:CDS
TTTAAATCCTTAAGTTGCAA+TGG 0.505721 1_4:+2100641 None:intergenic
AGTAGCTATGCTAAATATTG+TGG 0.506191 1_4:-2100520 Msa0143170:three_prime_UTR
TGCTTCATTATGGCTTGCAA+GGG 0.506316 1_4:-2101158 Msa0143170:CDS
AATGTGGTTGTGTTAGAACT+TGG 0.510674 1_4:-2101120 Msa0143170:CDS
CAAGGAAATTTATGATACTT+CGG 0.514673 1_4:+2101726 None:intergenic
AAAACGACACAAGAAAATCA+TGG 0.522256 1_4:+2100808 None:intergenic
GTTCGAGTTTGAATATGAGT+AGG 0.522810 1_4:-2100914 Msa0143170:exon
GATCAATTCAAAAGCATCAA+AGG 0.535278 1_4:+2101461 None:intergenic
TTGGCTAGCAATCCTATCTC+GGG 0.539654 1_4:-2101756 Msa0143170:CDS
CTCAATGATATTGAAATTAG+GGG 0.540853 1_4:+2101812 None:intergenic
TGTGTAACTTCATATTTATG+TGG 0.543217 1_4:-2101344 Msa0143170:CDS
CGAGTTTGAATATGAGTAGG+AGG 0.545250 1_4:-2100911 Msa0143170:exon
TCAAGTCATAACTTAAGGAA+AGG 0.545749 1_4:+2101190 None:intergenic
TATTATCTCCAATGTCAAGA+TGG 0.549282 1_4:+2101391 None:intergenic
TTGCATTTGATTTACAATTG+TGG 0.549574 1_4:-2100552 Msa0143170:three_prime_UTR
AAGTAGCAGAAGCATTGCTC+AGG 0.558819 1_4:-2100781 Msa0143170:three_prime_UTR
GCAAGAAATGGAGAGAAGCT+TGG 0.560253 1_4:-2102067 Msa0143170:CDS
AGTGTCATCCATCTTGACAT+TGG 0.562714 1_4:-2101399 Msa0143170:CDS
AGCAATGCTTCTGCTACTTG+AGG 0.564710 1_4:+2100785 None:intergenic
TACACACATCTACGATCTCA+AGG 0.565105 1_4:+2101361 None:intergenic
TTTCATTCAATACATCTGAC+TGG 0.565276 1_4:-2102025 Msa0143170:CDS
AATCGCTATGTCAGATTCAG+AGG 0.571141 1_4:-2101598 Msa0143170:CDS
ACTGAATTCATGATTCAACT+AGG 0.580130 1_4:-2100958 Msa0143170:CDS
ATCCCTAGCAGACCCAAGAT+GGG 0.587597 1_4:+2102112 None:intergenic
AATAATAGCTCGACGCGTGG+AGG 0.588508 1_4:-2103789 Msa0143170:CDS
GAGCAAATTTCGCGATATAG+GGG 0.592223 1_4:+2102002 None:intergenic
GGAGGATCTTCCTGTTGAAG+TGG 0.592625 1_4:-2102150 Msa0143170:CDS
AACGACAGCGACTTCAAGCA+AGG 0.592920 1_4:+2101708 None:intergenic
ATATCATTGAGAAATGCAGC+AGG 0.597773 1_4:-2101800 Msa0143170:CDS
TGGTTGTGTTAGAACTTGGA+TGG 0.599503 1_4:-2101116 Msa0143170:CDS
TTCTGGACAAACAATAGACA+AGG 0.600718 1_4:+2101620 None:intergenic
ATCCGGCAAGACGGACAAGA+AGG 0.600879 1_4:-2100843 Msa0143170:three_prime_UTR
AGGAATAATAGCTCGACGCG+TGG 0.607212 1_4:-2103792 Msa0143170:CDS
TGCTTGGCTTATGTATACGA+GGG 0.608384 1_4:-2101871 Msa0143170:CDS
CACAAGAAAATCATGGAACA+CGG 0.609378 1_4:+2100815 None:intergenic
TATTGAATGAAAGAGTTTGG+AGG 0.609653 1_4:+2102036 None:intergenic
ATTTCGAAATATAGATCGAA+GGG 0.610428 1_4:+2100676 None:intergenic
TATTTCGAAATATAGATCGA+AGG 0.611854 1_4:+2100675 None:intergenic
TGATAAACTCATATTAGTGG+CGG 0.615767 1_4:-2101517 Msa0143170:CDS
GGATTACTGAGGGACTCTTG+TGG 0.623079 1_4:-2103768 Msa0143170:CDS
ACATAAGCCAAGCAATGACA+GGG 0.628082 1_4:+2101880 None:intergenic
TTACTGAGGGACTCTTGTGG+TGG 0.628893 1_4:-2103765 Msa0143170:CDS
GATCTTCCTGTTGAAGTGGT+CGG 0.634333 1_4:-2102146 Msa0143170:CDS
TTTGATCTCATCCGGCAAGA+CGG 0.637709 1_4:-2100852 Msa0143170:three_prime_UTR
TTGCTTGGCTTATGTATACG+AGG 0.648455 1_4:-2101872 Msa0143170:CDS
CGAGATAGGATTGCTAGCCA+AGG 0.649589 1_4:+2101758 None:intergenic
TCATAGGTACCATTAGATGG+AGG 0.655418 1_4:+2103843 None:intergenic
GACGCGTGGAGGATTACTGA+GGG 0.673117 1_4:-2103778 Msa0143170:CDS
TGTTGTGGTCCTAAATACTG+TGG 0.680479 1_4:-2100609 Msa0143170:three_prime_UTR
TTGACAGCAACAGATGCATG+TGG 0.715644 1_4:+2100883 None:intergenic
CGACGCGTGGAGGATTACTG+AGG 0.729954 1_4:-2103779 Msa0143170:CDS
TGTGGTCCTAAATACTGTGG+AGG 0.739502 1_4:-2100606 Msa0143170:three_prime_UTR
CATAAGCCAAGCAATGACAG+GGG 0.741525 1_4:+2101881 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAATTGTTGTTATTTTTTT+TGG - chr1_4:2101714-2101733 Msa0143170:CDS 10.0%
!! TACAATAAAATCTTTCATTA+AGG + chr1_4:2100612-2100631 None:intergenic 15.0%
!! TTTAAGAAATAATCTTATCA+AGG + chr1_4:2100650-2100669 None:intergenic 15.0%
!! TTAACATATTAAAATGAGAA+GGG + chr1_4:2101041-2101060 None:intergenic 15.0%
!! ATTAACATATTAAAATGAGA+AGG + chr1_4:2101042-2101061 None:intergenic 15.0%
!! TTGAATAATTAAAATGAAAG+TGG + chr1_4:2101554-2101573 None:intergenic 15.0%
!!! ATAATAACCTATTCTAAAAG+AGG + chr1_4:2100735-2100754 None:intergenic 20.0%
!! ATTTAGCTAACATAAAAGTT+GGG + chr1_4:2100810-2100829 None:intergenic 20.0%
!! TATTTAGCTAACATAAAAGT+TGG + chr1_4:2100811-2100830 None:intergenic 20.0%
!!! AACTTTTATGTTAGCTAAAT+AGG - chr1_4:2100810-2100829 Msa0143170:three_prime_UTR 20.0%
!!! ACTTTTATGTTAGCTAAATA+GGG - chr1_4:2100811-2100830 Msa0143170:three_prime_UTR 20.0%
!! AAAATTAGGTATATATTCAG+AGG - chr1_4:2100992-2101011 Msa0143170:CDS 20.0%
!! AAATTAGGTATATATTCAGA+GGG - chr1_4:2100993-2101012 Msa0143170:CDS 20.0%
!!! TCTTTGAAAAAATAAATGCA+TGG + chr1_4:2101067-2101086 None:intergenic 20.0%
!! TATAGAGATAGTTTCTTATT+GGG + chr1_4:2101173-2101192 None:intergenic 20.0%
!! ATATAGAGATAGTTTCTTAT+TGG + chr1_4:2101174-2101193 None:intergenic 20.0%
!! TGTATATATGTTATAAACTG+TGG - chr1_4:2101215-2101234 Msa0143170:CDS 20.0%
!! CATATATCATATATCTTTAG+AGG - chr1_4:2101250-2101269 Msa0143170:CDS 20.0%
!!! TTTTATCAATGAAATGACAA+GGG + chr1_4:2101670-2101689 None:intergenic 20.0%
!!! ATTTTATCAATGAAATGACA+AGG + chr1_4:2101671-2101690 None:intergenic 20.0%
!!! TTGTTGTTATTTTTTTTGGT+AGG - chr1_4:2101718-2101737 Msa0143170:CDS 20.0%
!! TTCAATTAATCATAATGGTT+CGG + chr1_4:2101978-2101997 None:intergenic 20.0%
!! ACACTTTCAATTAATCATAA+TGG + chr1_4:2101983-2102002 None:intergenic 20.0%
!!! ACTGTTTTTGATTGAAATAT+AGG - chr1_4:2102154-2102173 Msa0143170:CDS 20.0%
!!! TGTTAAATTATTTTATGCGA+TGG - chr1_4:2102180-2102199 Msa0143170:intron 20.0%
!! TCTCAATGATATTGAAATTA+GGG + chr1_4:2102543-2102562 None:intergenic 20.0%
!! TTCTCAATGATATTGAAATT+AGG + chr1_4:2102544-2102563 None:intergenic 20.0%
!!! TTTTGATAAACTCATATTAG+TGG - chr1_4:2102831-2102850 Msa0143170:intron 20.0%
! AAGAAATAATCTTATCAAGG+TGG + chr1_4:2100647-2100666 None:intergenic 25.0%
!!! CATAAAACCTCTTTTAGAAT+AGG - chr1_4:2100725-2100744 Msa0143170:three_prime_UTR 25.0%
! AAAAAATTACGACAGAATGA+AGG + chr1_4:2100847-2100866 None:intergenic 25.0%
! TTAACTAATTAGATGGAGAA+TGG + chr1_4:2100905-2100924 None:intergenic 25.0%
! ACTTGCATTAACTAATTAGA+TGG + chr1_4:2100912-2100931 None:intergenic 25.0%
! AAAACCAAACTCGTAAAATT+AGG - chr1_4:2100978-2100997 Msa0143170:CDS 25.0%
!! TATACCTAATTTTACGAGTT+TGG + chr1_4:2100985-2101004 None:intergenic 25.0%
! AAGGAAAAATTGAAATCGAT+CGG + chr1_4:2101471-2101490 None:intergenic 25.0%
!!! TTTACCAATTTTTTATGCAG+AGG + chr1_4:2101596-2101615 None:intergenic 25.0%
!!! TAAATTATTTTATGCGATGG+AGG - chr1_4:2102183-2102202 Msa0143170:intron 25.0%
! ATGTATTGAATGAAAGAGTT+TGG + chr1_4:2102321-2102340 None:intergenic 25.0%
! CTCAATGATATTGAAATTAG+GGG + chr1_4:2102542-2102561 None:intergenic 25.0%
! CAAGGAAATTTATGATACTT+CGG + chr1_4:2102628-2102647 None:intergenic 25.0%
!! TGCTTTTGAATTGATCAATA+AGG - chr1_4:2102897-2102916 Msa0143170:intron 25.0%
!! GCTTTTGAATTGATCAATAA+GGG - chr1_4:2102898-2102917 Msa0143170:intron 25.0%
!! CTTTTGAATTGATCAATAAG+GGG - chr1_4:2102899-2102918 Msa0143170:intron 25.0%
! TGTGTAACTTCATATTTATG+TGG - chr1_4:2103007-2103026 Msa0143170:intron 25.0%
!! ATCTTTCAAGTCATAACTTA+AGG + chr1_4:2103169-2103188 None:intergenic 25.0%
! TTAAGTTATGACTTGAAAGA+TGG - chr1_4:2103168-2103187 Msa0143170:intron 25.0%
! ATGTCCTAATCTTAAAAAGA+TGG - chr1_4:2103308-2103327 Msa0143170:intron 25.0%
!!! ATGACCATCTTTTTAAGATT+AGG + chr1_4:2103315-2103334 None:intergenic 25.0%
! CTTAAAAAGATGGTCATTTA+TGG - chr1_4:2103318-2103337 Msa0143170:intron 25.0%
! AATCATGAATTCAGTGAATT+TGG + chr1_4:2103388-2103407 None:intergenic 25.0%
! ATTTCGAAATATAGATCGAA+GGG + chr1_4:2103678-2103697 None:intergenic 25.0%
! TATTTCGAAATATAGATCGA+AGG + chr1_4:2103679-2103698 None:intergenic 25.0%
!! TTTAAATCCTTAAGTTGCAA+TGG + chr1_4:2103713-2103732 None:intergenic 25.0%
! TGCAACTTAAGGATTTAAAT+TGG - chr1_4:2103714-2103733 Msa0143170:intron 25.0%
! GCAACTTAAGGATTTAAATT+GGG - chr1_4:2103715-2103734 Msa0143170:intron 25.0%
! CAACTTAAGGATTTAAATTG+GGG - chr1_4:2103716-2103735 Msa0143170:intron 25.0%
! TTGTAAATCAAATGCAATCA+AGG + chr1_4:2103796-2103815 None:intergenic 25.0%
!! TTGCATTTGATTTACAATTG+TGG - chr1_4:2103799-2103818 Msa0143170:CDS 25.0%
AATTCATAGGTACCATTAGA+TGG + chr1_4:2100514-2100533 None:intergenic 30.0%
CCAATCTACATTGAATTCAT+AGG + chr1_4:2100527-2100546 None:intergenic 30.0%
CCTATGAATTCAATGTAGAT+TGG - chr1_4:2100524-2100543 Msa0143170:three_prime_UTR 30.0%
AAATAATCTTATCAAGGTGG+AGG + chr1_4:2100644-2100663 None:intergenic 30.0%
TTCTTAAACAAGTTCCACTT+TGG - chr1_4:2100662-2100681 Msa0143170:three_prime_UTR 30.0%
!! AAGAGGTTTTATGTGAGTAA+TGG + chr1_4:2100718-2100737 None:intergenic 30.0%
!! GATATTCAGATCGATTTGAT+CGG - chr1_4:2101132-2101151 Msa0143170:CDS 30.0%
AGGAAAAATTGAAATCGATC+GGG + chr1_4:2101470-2101489 None:intergenic 30.0%
ACGAAAGATAACAGTTAGAA+GGG + chr1_4:2101521-2101540 None:intergenic 30.0%
AACGAAAGATAACAGTTAGA+AGG + chr1_4:2101522-2101541 None:intergenic 30.0%
ACATCCTCTGCATAAAAAAT+TGG - chr1_4:2101589-2101608 Msa0143170:CDS 30.0%
ATGTGAGTGAATTAGTATCT+GGG + chr1_4:2101638-2101657 None:intergenic 30.0%
!! ACTGTTTCTGATTGATATCT+TGG - chr1_4:2101755-2101774 Msa0143170:CDS 30.0%
TTTCTGATTGATATCTTGGT+CGG - chr1_4:2101759-2101778 Msa0143170:CDS 30.0%
AAAATAAATAAAGGGCAACC+CGG + chr1_4:2102053-2102072 None:intergenic 30.0%
GTATCATATTATCCCATCTT+GGG - chr1_4:2102227-2102246 Msa0143170:intron 30.0%
TATTGAATGAAAGAGTTTGG+AGG + chr1_4:2102318-2102337 None:intergenic 30.0%
TTTCATTCAATACATCTGAC+TGG - chr1_4:2102326-2102345 Msa0143170:intron 30.0%
!!! TGTACACTTTTTCAAACCAA+AGG - chr1_4:2102397-2102416 Msa0143170:intron 30.0%
CATCATCCATGAAAATTGTT+AGG + chr1_4:2102432-2102451 None:intergenic 30.0%
!! ATAGACAAGGTTTCAAGTTT+CGG + chr1_4:2102721-2102740 None:intergenic 30.0%
TGATAAACTCATATTAGTGG+CGG - chr1_4:2102834-2102853 Msa0143170:intron 30.0%
!! GATCAATTCAAAAGCATCAA+AGG + chr1_4:2102893-2102912 None:intergenic 30.0%
TATTATCTCCAATGTCAAGA+TGG + chr1_4:2102963-2102982 None:intergenic 30.0%
TCTAGGAAAACAAACAGATA+TGG + chr1_4:2103133-2103152 None:intergenic 30.0%
TCAAGTCATAACTTAAGGAA+AGG + chr1_4:2103164-2103183 None:intergenic 30.0%
ACTGAATTCATGATTCAACT+AGG - chr1_4:2103393-2103412 Msa0143170:intron 30.0%
AAAACGACACAAGAAAATCA+TGG + chr1_4:2103546-2103565 None:intergenic 30.0%
!! AAGTTGCAATGGTGTTATAT+TGG + chr1_4:2103702-2103721 None:intergenic 30.0%
TATAACACCATTGCAACTTA+AGG - chr1_4:2103703-2103722 Msa0143170:intron 30.0%
AGGATCTTAATCAAACAGTT+TGG - chr1_4:2103765-2103784 Msa0143170:CDS 30.0%
AGTAGCTATGCTAAATATTG+TGG - chr1_4:2103831-2103850 Msa0143170:CDS 30.0%
!! TAGATTGGTCTCGTATTAAG+AGG - chr1_4:2100539-2100558 Msa0143170:three_prime_UTR 35.0%
TATTATTCTCCTCAGTCTCT+TGG - chr1_4:2100749-2100768 Msa0143170:three_prime_UTR 35.0%
! TTTTAACGTCCTCCTTTTGT+TGG - chr1_4:2100864-2100883 Msa0143170:three_prime_UTR 35.0%
AACTCCAACAATCCAACAAA+AGG + chr1_4:2100879-2100898 None:intergenic 35.0%
!!! CGAGTTTGGTTTTTGTAAGT+GGG + chr1_4:2100971-2100990 None:intergenic 35.0%
!!! ACGAGTTTGGTTTTTGTAAG+TGG + chr1_4:2100972-2100991 None:intergenic 35.0%
TATCCTCTACAATGACTGTA+TGG + chr1_4:2101106-2101125 None:intergenic 35.0%
GGTGGAAAGTTAAAATGAGT+GGG + chr1_4:2101365-2101384 None:intergenic 35.0%
GGAAAAATTGAAATCGATCG+GGG + chr1_4:2101469-2101488 None:intergenic 35.0%
TACTTGGATGATTAAAGGGT+CGG + chr1_4:2101495-2101514 None:intergenic 35.0%
GGCTTACTTGGATGATTAAA+GGG + chr1_4:2101499-2101518 None:intergenic 35.0%
CGAAAGATAACAGTTAGAAG+GGG + chr1_4:2101520-2101539 None:intergenic 35.0%
CATGTGAGTGAATTAGTATC+TGG + chr1_4:2101639-2101658 None:intergenic 35.0%
GATATCAATCAGAAACAGTC+TGG + chr1_4:2101754-2101773 None:intergenic 35.0%
GATTTGTGATTGAATGATGC+TGG + chr1_4:2101887-2101906 None:intergenic 35.0%
GGTATCATATTATCCCATCT+TGG - chr1_4:2102226-2102245 Msa0143170:intron 35.0%
!! CATCCTTAACTTGCAAGAAA+TGG - chr1_4:2102272-2102291 Msa0143170:intron 35.0%
TCTCCATTTCTTGCAAGTTA+AGG + chr1_4:2102278-2102297 None:intergenic 35.0%
AGAGCAAATTTCGCGATATA+GGG + chr1_4:2102353-2102372 None:intergenic 35.0%
TAGAGCAAATTTCGCGATAT+AGG + chr1_4:2102354-2102373 None:intergenic 35.0%
ATATCATTGAGAAATGCAGC+AGG - chr1_4:2102551-2102570 Msa0143170:intron 35.0%
TTCTGGACAAACAATAGACA+AGG + chr1_4:2102734-2102753 None:intergenic 35.0%
CGTCGAAGTTAAAAAAGCTT+CGG - chr1_4:2103058-2103077 Msa0143170:intron 35.0%
! AAAGATGGAGTGCTTCATTA+TGG - chr1_4:2103183-2103202 Msa0143170:intron 35.0%
! GTTGTCTTTTCTGATGAATG+TGG - chr1_4:2103215-2103234 Msa0143170:intron 35.0%
AATGTGGTTGTGTTAGAACT+TGG - chr1_4:2103231-2103250 Msa0143170:intron 35.0%
! TCAGTGATCTTTTCTCAGTT+TGG - chr1_4:2103265-2103284 Msa0143170:intron 35.0%
! CAGTGATCTTTTCTCAGTTT+GGG - chr1_4:2103266-2103285 Msa0143170:intron 35.0%
! GTTCGAGTTTGAATATGAGT+AGG - chr1_4:2103437-2103456 Msa0143170:intron 35.0%
TTGAATATGAGTAGGAGGTT+CGG - chr1_4:2103445-2103464 Msa0143170:intron 35.0%
ATCTGTTGCTGTCAAAGTAT+TGG - chr1_4:2103476-2103495 Msa0143170:intron 35.0%
!! AGTATTGGTTTGATCTCATC+CGG - chr1_4:2103491-2103510 Msa0143170:intron 35.0%
CACAAGAAAATCATGGAACA+CGG + chr1_4:2103539-2103558 None:intergenic 35.0%
TAAGATCCTCCACAGTATTT+AGG + chr1_4:2103754-2103773 None:intergenic 35.0%
TCATAGGTACCATTAGATGG+AGG + chr1_4:2100511-2100530 None:intergenic 40.0%
TTCCACTTTGGTTGCAAATG+CGG - chr1_4:2100674-2100693 Msa0143170:three_prime_UTR 40.0%
TAAACCAATCCAAGAGACTG+AGG + chr1_4:2100761-2100780 None:intergenic 40.0%
! TCCTCCTTTTGTTGGATTGT+TGG - chr1_4:2100872-2100891 Msa0143170:three_prime_UTR 40.0%
TCCAACAATCCAACAAAAGG+AGG + chr1_4:2100876-2100895 None:intergenic 40.0%
!! GTTTTTGTAAGTGGGTATCG+TGG + chr1_4:2100963-2100982 None:intergenic 40.0%
GAACCATACAGTCATTGTAG+AGG - chr1_4:2101100-2101119 Msa0143170:CDS 40.0%
GGGTGGAAAGTTAAAATGAG+TGG + chr1_4:2101366-2101385 None:intergenic 40.0%
GGGCTTACTTGGATGATTAA+AGG + chr1_4:2101500-2101519 None:intergenic 40.0%
TGATATCTTGGTCGGTGTAA+TGG - chr1_4:2101767-2101786 Msa0143170:CDS 40.0%
ATGATACCGATCACTTCAAC+AGG + chr1_4:2101806-2101825 None:intergenic 40.0%
GAAAAGTCTTGACACTATCC+CGG - chr1_4:2102003-2102022 Msa0143170:CDS 40.0%
GAGCAAATTTCGCGATATAG+GGG + chr1_4:2102352-2102371 None:intergenic 40.0%
! CGAAATTTGCTCTAGTCACT+TGG - chr1_4:2102360-2102379 Msa0143170:intron 40.0%
ACATAAGCCAAGCAATGACA+GGG + chr1_4:2102474-2102493 None:intergenic 40.0%
TACATAAGCCAAGCAATGAC+AGG + chr1_4:2102475-2102494 None:intergenic 40.0%
TTGCTTGGCTTATGTATACG+AGG - chr1_4:2102479-2102498 Msa0143170:intron 40.0%
TGCTTGGCTTATGTATACGA+GGG - chr1_4:2102480-2102499 Msa0143170:intron 40.0%
GAAATGCAGCAGGAAAAAGT+TGG - chr1_4:2102561-2102580 Msa0143170:intron 40.0%
!! AAAGTTGGAGGTTTTGACCT+TGG - chr1_4:2102576-2102595 Msa0143170:intron 40.0%
! TTTTGTTAGCATCTCAGCGT+TGG - chr1_4:2102669-2102688 Msa0143170:intron 40.0%
GAATCTGACATAGCGATTTC+TGG + chr1_4:2102751-2102770 None:intergenic 40.0%
AATCGCTATGTCAGATTCAG+AGG - chr1_4:2102753-2102772 Msa0143170:intron 40.0%
AGTGTCATCCATCTTGACAT+TGG - chr1_4:2102952-2102971 Msa0143170:intron 40.0%
TACACACATCTACGATCTCA+AGG + chr1_4:2102993-2103012 None:intergenic 40.0%
AGTTAAAAAAGCTTCGGCTG+TGG - chr1_4:2103064-2103083 Msa0143170:intron 40.0%
! AAGGAAAGGTGCGTTAATCT+AGG + chr1_4:2103150-2103169 None:intergenic 40.0%
! TGCTTCATTATGGCTTGCAA+GGG - chr1_4:2103193-2103212 Msa0143170:intron 40.0%
TGGTTGTGTTAGAACTTGGA+TGG - chr1_4:2103235-2103254 Msa0143170:intron 40.0%
CGAGTTTGAATATGAGTAGG+AGG - chr1_4:2103440-2103459 Msa0143170:intron 40.0%
TTTAAATTGGGGTCGTGTTG+TGG - chr1_4:2103727-2103746 Msa0143170:intron 40.0%
TGTTGTGGTCCTAAATACTG+TGG - chr1_4:2103742-2103761 Msa0143170:intron 40.0%
TCCACTTTGGTTGCAAATGC+GGG - chr1_4:2100675-2100694 Msa0143170:three_prime_UTR 45.0%
TTCTCCTCAGTCTCTTGGAT+TGG - chr1_4:2100754-2100773 Msa0143170:three_prime_UTR 45.0%
GACGAAGGGAAAATTTGGCT+GGG + chr1_4:2101386-2101405 None:intergenic 45.0%
AGACGAAGGGAAAATTTGGC+TGG + chr1_4:2101387-2101406 None:intergenic 45.0%
CCTCAGACGAAGGGAAAATT+TGG + chr1_4:2101391-2101410 None:intergenic 45.0%
! CCAAATTTTCCCTTCGTCTG+AGG - chr1_4:2101388-2101407 Msa0143170:CDS 45.0%
AGAGCTGGAAGAAGAAGAAG+CGG + chr1_4:2101420-2101439 None:intergenic 45.0%
ACAGTTAGAAGGGGCTTACT+TGG + chr1_4:2101511-2101530 None:intergenic 45.0%
!! TCTTGGTCGGTGTAATGGAT+TGG - chr1_4:2101772-2101791 Msa0143170:CDS 45.0%
ACCCTTCCTGTTGAAGTGAT+CGG - chr1_4:2101797-2101816 Msa0143170:CDS 45.0%
ACCGATCACTTCAACAGGAA+GGG + chr1_4:2101801-2101820 None:intergenic 45.0%
TACCGATCACTTCAACAGGA+AGG + chr1_4:2101802-2101821 None:intergenic 45.0%
GATCTTCCTGTTGAAGTGGT+CGG - chr1_4:2102205-2102224 Msa0143170:intron 45.0%
ATGATACCGACCACTTCAAC+AGG + chr1_4:2102214-2102233 None:intergenic 45.0%
GCAAGAAATGGAGAGAAGCT+TGG - chr1_4:2102284-2102303 Msa0143170:intron 45.0%
! GCAGCGCCTAACAATTTTCA+TGG - chr1_4:2102423-2102442 Msa0143170:intron 45.0%
CATAAGCCAAGCAATGACAG+GGG + chr1_4:2102473-2102492 None:intergenic 45.0%
ATGCAGCAGGAAAAAGTTGG+AGG - chr1_4:2102564-2102583 Msa0143170:intron 45.0%
CTTGGCTAGCAATCCTATCT+CGG - chr1_4:2102594-2102613 Msa0143170:intron 45.0%
TTGGCTAGCAATCCTATCTC+GGG - chr1_4:2102595-2102614 Msa0143170:intron 45.0%
GCTTAGCAGCTCTTCTTTGA+AGG - chr1_4:2102786-2102805 Msa0143170:intron 45.0%
!!! GAGTGTTGTTTTTGACGACG+AGG - chr1_4:2103086-2103105 Msa0143170:intron 45.0%
!! TGTTTTTGACGACGAGGATG+AGG - chr1_4:2103092-2103111 Msa0143170:intron 45.0%
! GTGCTTCATTATGGCTTGCA+AGG - chr1_4:2103192-2103211 Msa0143170:intron 45.0%
! CTTTTCTCAGTTTGGGTAGC+TGG - chr1_4:2103273-2103292 Msa0143170:intron 45.0%
! AGTTTGGGTAGCTGGACTTT+TGG - chr1_4:2103281-2103300 Msa0143170:intron 45.0%
TTGACAGCAACAGATGCATG+TGG + chr1_4:2103471-2103490 None:intergenic 45.0%
TTTGATCTCATCCGGCAAGA+CGG - chr1_4:2103499-2103518 Msa0143170:intron 45.0%
! AGCAATGCTTCTGCTACTTG+AGG + chr1_4:2103569-2103588 None:intergenic 45.0%
!! AAGTAGCAGAAGCATTGCTC+AGG - chr1_4:2103570-2103589 Msa0143170:intron 45.0%
TGTGGTCCTAAATACTGTGG+AGG - chr1_4:2103745-2103764 Msa0143170:CDS 45.0%
!! AGAATAAAAAAATAAATAAA+GGG + chr1_4:2102061-2102080 None:intergenic 5.0%
!! AAGAATAAAAAAATAAATAA+AGG + chr1_4:2102062-2102081 None:intergenic 5.0%
AGGAATAATAGCTCGACGCG+TGG - chr1_4:2100559-2100578 Msa0143170:three_prime_UTR 50.0%
AATAATAGCTCGACGCGTGG+AGG - chr1_4:2100562-2100581 Msa0143170:three_prime_UTR 50.0%
GGATTACTGAGGGACTCTTG+TGG - chr1_4:2100583-2100602 Msa0143170:three_prime_UTR 50.0%
TTACTGAGGGACTCTTGTGG+TGG - chr1_4:2100586-2100605 Msa0143170:three_prime_UTR 50.0%
ACCCGCATTTGCAACCAAAG+TGG + chr1_4:2100679-2100698 None:intergenic 50.0%
CGTGTTCTATGTCACGTGCT+AGG + chr1_4:2101333-2101352 None:intergenic 50.0%
GAAGGGAAAATTTGGCTGGG+TGG + chr1_4:2101383-2101402 None:intergenic 50.0%
GGATGATTAAAGGGTCGGCA+AGG + chr1_4:2101490-2101509 None:intergenic 50.0%
GGAGGATCTTCCTGTTGAAG+TGG - chr1_4:2102201-2102220 Msa0143170:intron 50.0%
TATCCCATCTTGGGTCTGCT+AGG - chr1_4:2102236-2102255 Msa0143170:intron 50.0%
ATCCCATCTTGGGTCTGCTA+GGG - chr1_4:2102237-2102256 Msa0143170:intron 50.0%
ATCCCTAGCAGACCCAAGAT+GGG + chr1_4:2102242-2102261 None:intergenic 50.0%
! TGTTAGGCGCTGCAATCCTT+TGG + chr1_4:2102416-2102435 None:intergenic 50.0%
CTGTTACCCCTGTCATTGCT+TGG - chr1_4:2102464-2102483 Msa0143170:intron 50.0%
CGAGATAGGATTGCTAGCCA+AGG + chr1_4:2102596-2102615 None:intergenic 50.0%
AACGACAGCGACTTCAAGCA+AGG + chr1_4:2102646-2102665 None:intergenic 50.0%
! TGGGTAGCTGGACTTTTGGA+AGG - chr1_4:2103285-2103304 Msa0143170:intron 50.0%
TACCTTCTTGTCCGTCTTGC+CGG + chr1_4:2103513-2103532 None:intergenic 50.0%
GACGCGTGGAGGATTACTGA+GGG - chr1_4:2100573-2100592 Msa0143170:three_prime_UTR 55.0%
GAGAGAGAGAGAGAGAGAGC+TGG + chr1_4:2101435-2101454 None:intergenic 55.0%
CATCCCTAGCAGACCCAAGA+TGG + chr1_4:2102243-2102262 None:intergenic 55.0%
TTCGGCTCAACACCCGAGAT+AGG + chr1_4:2102610-2102629 None:intergenic 55.0%
ATCCGGCAAGACGGACAAGA+AGG - chr1_4:2103508-2103527 Msa0143170:intron 55.0%
CGACGCGTGGAGGATTACTG+AGG - chr1_4:2100572-2100591 Msa0143170:three_prime_UTR 60.0%
CGGCTCTCTCCTCAGACGAA+GGG + chr1_4:2101400-2101419 None:intergenic 60.0%
TATCCCGGACCCTGCGTAAG+CGG - chr1_4:2102018-2102037 Msa0143170:CDS 60.0%
GCGGCTCTCTCCTCAGACGA+AGG + chr1_4:2101401-2101420 None:intergenic 65.0%
ATCCCGGACCCTGCGTAAGC+GGG - chr1_4:2102019-2102038 Msa0143170:CDS 65.0%
TGCACGCTCCCGCTTACGCA+GGG + chr1_4:2102030-2102049 None:intergenic 65.0%
CTCCCGCTTACGCAGGGTCC+GGG + chr1_4:2102024-2102043 None:intergenic 70.0%
GCTCCCGCTTACGCAGGGTC+CGG + chr1_4:2102025-2102044 None:intergenic 70.0%
GTGCACGCTCCCGCTTACGC+AGG + chr1_4:2102031-2102050 None:intergenic 70.0%
! GCGTAAGCGGGAGCGTGCAC+CGG - chr1_4:2102031-2102050 Msa0143170:CDS 70.0%
! CGTAAGCGGGAGCGTGCACC+GGG - chr1_4:2102032-2102051 Msa0143170:CDS 70.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 2100502 2103871 2100502 ID=Msa0143170;Name=Msa0143170
chr1_4 mRNA 2100502 2103871 2100502 ID=Msa0143170-mRNA-1;Parent=Msa0143170;Name=Msa0143170-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|0|0.5|0.5|1|1|2|413|469
chr1_4 exon 2100502 2102197 2100502 ID=Msa0143170-mRNA-1:exon:854;Parent=Msa0143170-mRNA-1
chr1_4 exon 2103745 2103871 2103745 ID=Msa0143170-mRNA-1:exon:853;Parent=Msa0143170-mRNA-1
chr1_4 CDS 2103745 2103871 2103745 ID=Msa0143170-mRNA-1:cds;Parent=Msa0143170-mRNA-1
chr1_4 CDS 2100915 2102197 2100915 ID=Msa0143170-mRNA-1:cds;Parent=Msa0143170-mRNA-1
chr1_4 three_prime_UTR 2100502 2100914 2100502 ID=Msa0143170-mRNA-1:three_prime_utr;Parent=Msa0143170-mRNA-1
Gene Sequence

>Msa0143170

ATGAATCCTCCATCTAATGGTACCTATGAATTCAATGTAGATTGGTCTCGTATTAAGAGGAATAATAGCTCGACGCGTGGAGGATTACTGAGGGACTCTTGTGGTGGCCTTAATGAAAGATTTTATTGTTCTGTTAAATTATTTTATGCGATGGAGGATCTTCCTGTTGAAGTGGTCGGTATCATATTATCCCATCTTGGGTCTGCTAGGGATGTTCTGATTGCATCCTTAACTTGCAAGAAATGGAGAGAAGCTTGGCAGAATCACCTCCAAACTCTTTCATTCAATACATCTGACTGGCCCCTATATCGCGAAATTTGCTCTAGTCACTTGGAAATGCTCATTACTTGTACACTTTTTCAAACCAAAGGATTGCAGCGCCTAACAATTTTCATGGATGATGAGCATGAGTTTTCTGTTACCCCTGTCATTGCTTGGCTTATGTATACGAGGGATTCATTGCGCGAGCTACGTTATAATGTGAGAACATCCCCTAATTTCAATATCATTGAGAAATGCAGCAGGAAAAAGTTGGAGGTTTTGACCTTGGCTAGCAATCCTATCTCGGGTGTTGAGCCGAAGTATCATAAATTTCCTTGCTTGAAGTCGCTGTCGTTGAGTTTTGTTAGCATCTCAGCGTTGGATTTGAGTCTTCTGCTTTCTGCTTGTCCGAAACTTGAAACCTTGTCTATTGTTTGTCCAGAAATCGCTATGTCAGATTCAGAGGCTTCAATAGAGCTTAGCAGCTCTTCTTTGAAGGATTTCTTTGTCGAGTCATATAGTTTTGATAAACTCATATTAGTGGCGGATATGCTCGAGAATCTGCATCTGAAAGATTGTTCCTTTGATGCTTTTGAATTGATCAATAAGGGGACATTGAAAGTATTGAAGCTTGATGATGTTAGTGTCATCCATCTTGACATTGGAGATAATACAGAAAACCTTGAGATCGTAGATGTGTGTAACTTCATATTTATGTGGCAAAATTTTTATAATATGATCTCAAAAGCGTCGAAGTTAAAAAAGCTTCGGCTGTGGAGTGTTGTTTTTGACGACGAGGATGAGGTTGTTGATATAGAGACCATATCTGTTTGTTTTCCTAGATTAACGCACCTTTCCTTAAGTTATGACTTGAAAGATGGAGTGCTTCATTATGGCTTGCAAGGGTTGTCTTTTCTGATGAATGTGGTTGTGTTAGAACTTGGATGGACGACAATCAGTGATCTTTTCTCAGTTTGGGTAGCTGGACTTTTGGAAGGATGTCCTAATCTTAAAAAGATGGTCATTTATGGCTATGTTGCTGAGATCAAAACACATGAAGAGTGTCAAACTTTTACCAAATTCACTGAATTCATGATTCAACTAGGAAGAAAATATAGTCATATAAAGTTCGAGTTTGAATATGAGTAG
Protein sequence

>Msa0143170

MNPPSNGTYEFNVDWSRIKRNNSSTRGGLLRDSCGGLNERFYCSVKLFYAMEDLPVEVVGIILSHLGSARDVLIASLTCKKWREAWQNHLQTLSFNTSDWPLYREICSSHLEMLITCTLFQTKGLQRLTIFMDDEHEFSVTPVIAWLMYTRDSLRELRYNVRTSPNFNIIEKCSRKKLEVLTLASNPISGVEPKYHKFPCLKSLSLSFVSISALDLSLLLSACPKLETLSIVCPEIAMSDSEASIELSSSSLKDFFVESYSFDKLILVADMLENLHLKDCSFDAFELINKGTLKVLKLDDVSVIHLDIGDNTENLEIVDVCNFIFMWQNFYNMISKASKLKKLRLWSVVFDDEDEVVDIETISVCFPRLTHLSLSYDLKDGVLHYGLQGLSFLMNVVVLELGWTTISDLFSVWVAGLLEGCPNLKKMVIYGYVAEIKTHEECQTFTKFTEFMIQLGRKYSHIKFEFEYE*