AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1351370

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1351370 MtrunA17_Chr3g0126901 97.727 440 10 0 1 440 1 440 0.0 897
Msa1351370 MtrunA17_Chr1g0146931 57.078 438 144 12 40 436 49 483 4.44e-106 323
Msa1351370 MtrunA17_Chr8g0346021 32.857 420 195 16 1 363 1 390 2.44e-36 140
Msa1351370 MtrunA17_Chr6g0462191 39.236 288 139 8 1 272 1 268 1.00e-28 117
Msa1351370 MtrunA17_Chr7g0273861 28.758 459 224 19 1 404 1 411 1.24e-28 117
Msa1351370 MtrunA17_Chr1g0200161 37.640 178 85 4 112 275 141 306 9.46e-27 112
Msa1351370 MtrunA17_Chr4g0020671 25.575 391 234 12 1 368 1 357 6.20e-26 109
Msa1351370 MtrunA17_Chr1g0181581 26.591 440 241 18 1 372 1 426 6.82e-26 109
Msa1351370 MtrunA17_Chr7g0263731 35.517 290 146 10 1 272 1 267 2.05e-25 108
Msa1351370 MtrunA17_Chr4g0025441 38.660 194 77 6 107 281 88 258 2.33e-24 104
Msa1351370 MtrunA17_Chr4g0045641 26.609 466 236 15 1 372 1 454 1.91e-23 102
Msa1351370 MtrunA17_Chr1g0204901 58.779 131 48 3 56 180 74 204 4.29e-22 98.2
Msa1351370 MtrunA17_Chr1g0156121 34.884 172 68 5 112 273 124 261 7.21e-21 95.5
Msa1351370 MtrunA17_Chr8g0371131 60.526 76 29 1 109 184 68 142 8.49e-21 88.2
Msa1351370 MtrunA17_Chr6g0472551 34.343 198 94 5 114 311 119 280 3.02e-20 92.8
Msa1351370 MtrunA17_Chr1g0203711 63.768 69 25 0 112 180 115 183 1.60e-19 90.5
Msa1351370 MtrunA17_Chr3g0135851 42.857 168 88 5 112 272 91 257 1.67e-19 90.5
Msa1351370 MtrunA17_Chr1g0172611 28.571 399 219 11 4 376 3 361 1.91e-19 89.7
Msa1351370 MtrunA17_Chr8g0389781 58.442 77 27 1 101 172 110 186 4.92e-19 89.0
Msa1351370 MtrunA17_Chr8g0340761 43.137 204 98 5 1 188 1 202 7.84e-19 88.2
Msa1351370 MtrunA17_Chr7g0273951 58.571 70 29 0 111 180 105 174 2.38e-18 86.3
Msa1351370 MtrunA17_Chr4g0060381 35.862 145 72 3 110 247 98 228 2.66e-18 87.4
Msa1351370 MtrunA17_Chr2g0330921 52.174 92 37 1 97 181 75 166 2.17e-17 83.2
Msa1351370 MtrunA17_Chr1g0166431 56.579 76 32 1 90 164 94 169 6.40e-17 82.4
Msa1351370 MtrunA17_Chr2g0283321 30.939 181 105 6 114 286 65 233 4.02e-15 75.9
Msa1351370 MtrunA17_Chr4g0057321 58.209 67 27 1 114 179 90 156 2.19e-14 74.7
Msa1351370 MtrunA17_Chr2g0330051 35.664 143 76 6 141 272 107 244 2.12e-12 68.6
Msa1351370 MtrunA17_Chr5g0416161 32.386 176 72 7 110 274 117 256 9.81e-12 67.0
Msa1351370 MtrunA17_Chr2g0316401 26.257 179 106 5 114 280 76 240 1.16e-11 65.5
Msa1351370 MtrunA17_Chr7g0215371 52.632 57 27 0 112 168 19 75 1.86e-11 60.5
Msa1351370 MtrunA17_Chr8g0342551 59.036 83 32 1 110 190 88 170 3.39e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1351370 AT3G49260.1 47.333 450 190 17 1 408 1 445 1.09e-87 275
Msa1351370 AT3G49260.4 47.333 450 190 17 1 408 1 445 1.09e-87 275
Msa1351370 AT3G49260.2 47.333 450 190 17 1 408 1 445 1.09e-87 275
Msa1351370 AT3G49260.3 47.450 451 189 17 1 408 1 446 2.29e-87 274
Msa1351370 AT5G03040.1 30.189 424 218 13 1 363 1 407 3.72e-38 144
Msa1351370 AT5G03040.3 30.189 424 218 13 1 363 1 407 3.72e-38 144
Msa1351370 AT5G03040.2 30.189 424 218 13 1 363 1 407 3.72e-38 144
Msa1351370 AT3G52290.1 33.103 435 188 15 5 370 2 402 3.96e-37 140
Msa1351370 AT4G00820.1 32.239 335 179 11 63 373 85 395 3.68e-31 125
Msa1351370 AT1G01110.2 31.421 401 219 14 1 367 1 379 9.28e-31 124
Msa1351370 AT3G09710.1 31.104 299 142 10 4 272 3 267 8.51e-30 120
Msa1351370 AT3G09710.2 29.712 313 142 11 4 272 3 281 6.67e-28 115
Msa1351370 AT4G10640.1 28.358 402 217 14 1 367 1 366 5.06e-27 112
Msa1351370 AT2G26180.1 41.262 206 112 5 112 309 84 288 1.47e-26 111
Msa1351370 AT3G59690.2 31.317 281 148 10 115 367 172 435 1.27e-24 106
Msa1351370 AT3G59690.1 31.317 281 148 10 115 367 172 435 1.27e-24 106
Msa1351370 AT3G22190.3 41.799 189 81 4 105 281 81 252 5.26e-23 100
Msa1351370 AT3G22190.2 41.799 189 81 4 105 281 81 252 5.26e-23 100
Msa1351370 AT3G22190.4 41.799 189 81 4 105 281 81 252 5.26e-23 100
Msa1351370 AT3G22190.1 41.799 189 81 4 105 281 81 252 5.26e-23 100
Msa1351370 AT1G72670.1 29.178 377 221 12 1 357 1 351 1.33e-22 99.8
Msa1351370 AT5G13460.1 30.888 259 140 11 114 360 113 344 1.78e-22 99.8
Msa1351370 AT3G49380.1 39.896 193 84 6 1 178 1 176 2.76e-22 97.8
Msa1351370 AT4G23060.1 62.821 78 28 1 101 178 157 233 3.59e-21 96.3
Msa1351370 AT4G14750.1 66.667 66 22 0 112 177 105 170 7.65e-21 94.4
Msa1351370 AT4G14750.3 66.667 66 22 0 112 177 164 229 1.23e-20 94.4
Msa1351370 AT4G14750.2 66.667 66 22 0 112 177 164 229 1.24e-20 94.4
Msa1351370 AT1G17480.1 42.063 126 67 2 110 235 92 211 3.27e-20 92.0
Msa1351370 AT2G43680.4 28.014 282 160 9 114 368 324 589 1.13e-19 92.0
Msa1351370 AT2G43680.2 28.014 282 160 9 114 368 324 589 1.13e-19 92.0
Msa1351370 AT2G43680.3 28.014 282 160 9 114 368 324 589 1.13e-19 92.0
Msa1351370 AT2G43680.5 28.014 282 160 9 114 368 324 589 1.13e-19 92.0
Msa1351370 AT2G43680.1 28.014 282 160 9 114 368 325 590 1.19e-19 92.0
Msa1351370 AT3G16490.1 50.000 96 43 1 101 196 96 186 1.02e-18 88.2
Msa1351370 AT2G26410.2 34.254 181 92 6 109 275 145 312 1.27e-18 88.6
Msa1351370 AT2G26410.1 34.254 181 92 6 109 275 145 312 1.27e-18 88.6
Msa1351370 AT5G07240.1 57.647 85 23 1 101 172 82 166 2.42e-18 87.0
Msa1351370 AT5G07240.2 57.647 85 23 1 101 172 128 212 3.01e-18 87.0
Msa1351370 AT1G18840.1 55.385 65 29 0 112 176 109 173 8.14e-18 86.3
Msa1351370 AT1G18840.3 55.385 65 29 0 112 176 109 173 8.14e-18 86.3
Msa1351370 AT1G18840.2 55.385 65 29 0 112 176 109 173 8.14e-18 86.3
Msa1351370 AT1G19870.1 33.929 168 62 5 112 272 215 340 1.06e-17 86.3
Msa1351370 AT1G19870.2 33.929 168 62 5 112 272 32 157 1.09e-17 85.9
Msa1351370 AT3G51380.1 56.338 71 30 1 109 179 34 103 1.15e-17 78.6
Msa1351370 AT4G29150.1 56.522 69 30 0 106 174 125 193 6.78e-16 79.7
Msa1351370 AT1G51960.1 42.308 104 56 2 114 216 102 202 1.80e-15 77.8
Msa1351370 AT5G62070.1 71.698 53 15 0 114 166 119 171 8.69e-15 76.3
Msa1351370 AT1G74690.1 53.846 65 30 0 112 176 113 177 1.40e-14 76.3
Msa1351370 AT1G14380.1 50.000 64 32 0 111 174 93 156 2.46e-14 75.9
Msa1351370 AT1G14380.3 50.000 64 32 0 111 174 93 156 2.46e-14 75.9
Msa1351370 AT1G14380.4 50.000 64 32 0 111 174 93 156 2.46e-14 75.9
Msa1351370 AT1G14380.6 50.000 64 32 0 111 174 93 156 2.68e-14 75.5
Msa1351370 AT1G14380.5 50.000 64 32 0 111 174 93 156 2.68e-14 75.5
Msa1351370 AT1G14380.2 50.000 64 32 0 111 174 93 156 2.68e-14 75.5
Msa1351370 AT1G14380.7 50.000 64 32 0 111 174 93 156 3.49e-14 75.1
Msa1351370 AT2G33990.1 29.208 202 127 8 114 309 49 240 7.47e-14 72.0
Msa1351370 AT5G03960.3 34.568 243 134 7 4 231 3 235 5.64e-12 67.8
Msa1351370 AT5G03960.2 34.568 243 134 7 4 231 3 235 5.64e-12 67.8
Msa1351370 AT5G03960.1 39.037 187 99 4 60 231 48 234 1.53e-11 66.2
Msa1351370 AT3G15050.1 28.090 178 110 4 111 280 49 216 1.67e-11 64.7

Find 145 sgRNAs with CRISPR-Local

Find 281 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTTTAATTATTACTATTTA+TGG 0.027919 tig0012204:+31067 Msa1351370:three_prime_UTR
TCAAACAATGTTAGGCCTTT+AGG 0.179218 tig0012204:+30492 Msa1351370:CDS
GGGCTTGGGCTTCTAGTAAT+TGG 0.200692 tig0012204:-30864 None:intergenic
CCTTCAATCTAAACGGATTT+CGG 0.207622 tig0012204:+30905 Msa1351370:CDS
CAAACAATGTTAGGCCTTTA+GGG 0.216561 tig0012204:+30493 Msa1351370:CDS
GGGCGTCGTTTGGATCTTAC+TGG 0.267147 tig0012204:+30766 Msa1351370:CDS
CTTCAATCTAAACGGATTTC+GGG 0.269149 tig0012204:+30906 Msa1351370:CDS
AAATTAAGGACACTGATTTC+AGG 0.276524 tig0012204:+29755 Msa1351370:CDS
TGGCCTTTGCAGACTGTGTT+GGG 0.282948 tig0012204:-30713 None:intergenic
GACTTAAATCATGAAATTCT+TGG 0.286842 tig0012204:-30635 None:intergenic
CATCTTCCATAACAACAATT+AGG 0.288073 tig0012204:+26954 Msa1351370:five_prime_UTR
CTTCCTTCATTCGTGATATC+TGG 0.297331 tig0012204:-28890 None:intergenic
CTTTCACTATCATCAACATT+TGG 0.297842 tig0012204:-30402 None:intergenic
TGAAATCCTAATTGTTGTTA+TGG 0.298589 tig0012204:-26960 None:intergenic
TCAAAGTAGCCAGAAAATTA+AGG 0.332991 tig0012204:+29741 Msa1351370:CDS
AGCAGCAGAGGAGGTATCTA+TGG 0.333458 tig0012204:+28844 Msa1351370:CDS
ACGTGCACTGCGTGCTTTGA+AGG 0.337011 tig0012204:+29513 Msa1351370:CDS
CATGGTATAGTATAATTATA+AGG 0.337361 tig0012204:-31005 None:intergenic
ATATCATCAGTGGGTGTATA+TGG 0.341287 tig0012204:-30537 None:intergenic
CGTGTTTGTCGTGAGTCAAT+TGG 0.349060 tig0012204:-29654 None:intergenic
AACGACGCCCGATGCTTTGA+TGG 0.352089 tig0012204:-30753 None:intergenic
AGCAGCAGCTGAAGCTGCAG+TGG 0.352700 tig0012204:+28976 Msa1351370:CDS
AATGTTAGGCCTTTAGGGTT+GGG 0.372284 tig0012204:+30498 Msa1351370:CDS
ACATGGAGCTACAATGAATA+AGG 0.377871 tig0012204:+29780 Msa1351370:CDS
TTTCACTATCATCAACATTT+GGG 0.379669 tig0012204:-30401 None:intergenic
TGGGCCTTGACTCTTAATCT+TGG 0.382714 tig0012204:-30733 None:intergenic
CCAACATTCATCTCAGAAAC+AGG 0.383345 tig0012204:+27074 Msa1351370:five_prime_UTR
TCAAAGCATCGGGCGTCGTT+TGG 0.387573 tig0012204:+30756 Msa1351370:CDS
CCAACCAGTTCCAATCCAAT+TGG 0.388280 tig0012204:-30449 None:intergenic
AGAAGGAAAAGGAAGAGAAT+TGG 0.395607 tig0012204:+28813 Msa1351370:intron
AGCTGCTAGAGTTGTGAGAT+TGG 0.400903 tig0012204:+29012 Msa1351370:CDS
CCGAAATCCGTTTAGATTGA+AGG 0.401125 tig0012204:-30905 None:intergenic
AGATGAATGTTGGTAGTTAC+TGG 0.406597 tig0012204:-27064 None:intergenic
TATCACAATCAAACAATGTT+AGG 0.407700 tig0012204:+30484 Msa1351370:CDS
ACACAAATGGTTGGAACTAT+AGG 0.408666 tig0012204:+29716 Msa1351370:CDS
GCAAGCATTGGTAAGGGTTC+AGG 0.410589 tig0012204:+29609 Msa1351370:CDS
TGGAGGTGTACCTAGCTATA+TGG 0.419424 tig0012204:+30689 Msa1351370:CDS
TTGGCCTTTGCAGACTGTGT+TGG 0.420117 tig0012204:-30714 None:intergenic
TATGATTCATCTGGCTCTGG+TGG 0.422243 tig0012204:+30825 Msa1351370:CDS
GGCTATGATTCATCTGGCTC+TGG 0.428311 tig0012204:+30822 Msa1351370:CDS
TGAGCGTGAAAGAGCCCAAT+TGG 0.428420 tig0012204:+30434 Msa1351370:CDS
AGCTGAAGCTGCAGTGGCTG+CGG 0.436330 tig0012204:+28982 Msa1351370:CDS
TGCTGCTGTTGCTGCAGCAT+AGG 0.442444 tig0012204:-28957 None:intergenic
CGTTGGTGGTGTTTAGAGAT+AGG 0.443029 tig0012204:-30657 None:intergenic
GAGCCCAATTGGATTGGAAC+TGG 0.444353 tig0012204:+30445 Msa1351370:CDS
CGGGTGCTGGTGTTGAGGAT+TGG 0.453226 tig0012204:+30925 Msa1351370:CDS
GGGATGGGCTATGATTCATC+TGG 0.454170 tig0012204:+30816 Msa1351370:CDS
TCCATACGCTTTCAACTGCC+AGG 0.454990 tig0012204:+29813 Msa1351370:CDS
TGACAGTGACACAAAACATA+TGG 0.456294 tig0012204:-31157 None:intergenic
CTTGTCCAGCCATGACCTCC+AGG 0.458475 tig0012204:-30957 None:intergenic
AGGTCATGGACATAACTCTA+AGG 0.464310 tig0012204:+29036 Msa1351370:CDS
CAATGTTAGGCCTTTAGGGT+TGG 0.466914 tig0012204:+30497 Msa1351370:CDS
ATGATTCATCTGGCTCTGGT+GGG 0.467215 tig0012204:+30826 Msa1351370:CDS
ATTCATGGGACAGTAATGTT+TGG 0.472342 tig0012204:-27007 None:intergenic
CTCATTCAGTCCTACTATAG+AGG 0.474856 tig0012204:+29076 Msa1351370:CDS
ATGATTTCTCTCTTCAGTCA+AGG 0.475457 tig0012204:-28927 None:intergenic
TCTAAACGGATTTCGGGTGC+TGG 0.479590 tig0012204:+30912 Msa1351370:CDS
ATGTTAGGCCTTTAGGGTTG+GGG 0.482998 tig0012204:+30499 Msa1351370:CDS
ACGGAGTCCTCCGACTGGGA+TGG 0.488447 tig0012204:+30800 Msa1351370:CDS
GCTAGAGTTGTGAGATTGGC+AGG 0.489676 tig0012204:+29016 Msa1351370:CDS
TGATTTCTCTCTTCAGTCAA+GGG 0.491440 tig0012204:-28926 None:intergenic
TTTGCATGGATTGAGTCTCT+TGG 0.491563 tig0012204:-30597 None:intergenic
TTGTGTGTGGTCTAAACCTT+GGG 0.492357 tig0012204:+31030 Msa1351370:three_prime_UTR
TCCTCTCAGATGGGTAAGAA+AGG 0.494472 tig0012204:+27278 Msa1351370:exon
GGATTCGCCTTCAATCTAAA+CGG 0.496890 tig0012204:+30898 Msa1351370:CDS
GGCCTTTGCAGACTGTGTTG+GGG 0.500229 tig0012204:-30712 None:intergenic
AGTTACTGGTCCTTCATGTA+TGG 0.501378 tig0012204:-27050 None:intergenic
ACCTGGCAGTTGAAAGCGTA+TGG 0.501521 tig0012204:-29814 None:intergenic
TCAGCACGGAGTCCTCCGAC+TGG 0.503212 tig0012204:+30795 Msa1351370:CDS
CAACCAGTTCCAATCCAATT+GGG 0.509130 tig0012204:-30448 None:intergenic
CGGAGTCCTCCGACTGGGAT+GGG 0.510075 tig0012204:+30801 Msa1351370:CDS
TCAAGGCCCATCAAAGCATC+GGG 0.510279 tig0012204:+30746 Msa1351370:CDS
ATTGGAACTGGTTGGAGCGT+TGG 0.514467 tig0012204:+30457 Msa1351370:CDS
CATCAGTGGGTGTATATGGT+GGG 0.517012 tig0012204:-30533 None:intergenic
GTATATGGTGGGGTCTCAAG+TGG 0.518909 tig0012204:-30522 None:intergenic
TCAAGTGGCCCCAACCCTAA+AGG 0.519519 tig0012204:-30507 None:intergenic
GTTGTGAGATTGGCAGGTCA+TGG 0.520639 tig0012204:+29022 Msa1351370:CDS
ACGACAAAGTTAGACACAAA+TGG 0.522106 tig0012204:+29703 Msa1351370:CDS
ACATAGCCTTTCCTCTCAGA+TGG 0.522560 tig0012204:+27268 Msa1351370:exon
GATGCATGCAAGCATTGGTA+AGG 0.524234 tig0012204:+29602 Msa1351370:CDS
CAAACACGAGCGGACAGTAG+AGG 0.524630 tig0012204:+29669 Msa1351370:CDS
GTCAAGGCCCATCAAAGCAT+CGG 0.524983 tig0012204:+30745 Msa1351370:CDS
TCACCAGATATCACGAATGA+AGG 0.525050 tig0012204:+28887 Msa1351370:CDS
TCATGAGGCCCATGTTTGCA+TGG 0.531097 tig0012204:-30611 None:intergenic
CGTGCACTGCGTGCTTTGAA+GGG 0.531337 tig0012204:+29514 Msa1351370:CDS
CATGAAATTCTTGGTTCATG+AGG 0.532356 tig0012204:-30626 None:intergenic
GTGAAAGAGCCCAATTGGAT+TGG 0.532613 tig0012204:+30439 Msa1351370:CDS
TACTAGGTATCCTCTATAGT+AGG 0.537083 tig0012204:-29086 None:intergenic
TTCTTCAGACATATCATCAG+TGG 0.544019 tig0012204:-30547 None:intergenic
ATCAGTGGGTGTATATGGTG+GGG 0.545609 tig0012204:-30532 None:intergenic
CTCCACTGTGGTGTCGTTGG+TGG 0.546689 tig0012204:-30671 None:intergenic
GATTTCGGGTGCTGGTGTTG+AGG 0.548721 tig0012204:+30920 Msa1351370:CDS
CACCTCCACTGTGGTGTCGT+TGG 0.549460 tig0012204:-30674 None:intergenic
TGATTCATCTGGCTCTGGTG+GGG 0.551322 tig0012204:+30827 Msa1351370:CDS
TTTAGACCACACACAATACA+TGG 0.552448 tig0012204:-31023 None:intergenic
ACGACGCCCGATGCTTTGAT+GGG 0.553948 tig0012204:-30752 None:intergenic
TCTTCAGACATATCATCAGT+GGG 0.559544 tig0012204:-30546 None:intergenic
CAAAGTTAGACACAAATGGT+TGG 0.564649 tig0012204:+29707 Msa1351370:CDS
CTCAACTCTACAAGAGAACA+AGG 0.565237 tig0012204:+27352 Msa1351370:CDS
GACTCAATCCATGCAAACAT+GGG 0.565689 tig0012204:+30603 Msa1351370:CDS
ATATCTGGTGAACTATATGC+AGG 0.565870 tig0012204:-28875 None:intergenic
AGACTCAATCCATGCAAACA+TGG 0.566185 tig0012204:+30602 Msa1351370:CDS
TTTGTGTCTAACTTTGTCGT+AGG 0.567471 tig0012204:-29700 None:intergenic
CCAATTGGATTGGAACTGGT+TGG 0.567620 tig0012204:+30449 Msa1351370:CDS
TGAGATTGCAAGCACTTGTG+AGG 0.571803 tig0012204:+29542 Msa1351370:CDS
GAGATTGCAAGCACTTGTGA+GGG 0.574688 tig0012204:+29543 Msa1351370:CDS
TTCAGTCAAGGGTGTTGATG+TGG 0.575945 tig0012204:-28915 None:intergenic
TGGGTAAGAAAGGAAGCAGT+TGG 0.576166 tig0012204:+27288 Msa1351370:CDS
TGTGTTGGGGCCATATAGCT+AGG 0.577052 tig0012204:-30699 None:intergenic
TCATCAGTGGGTGTATATGG+TGG 0.580532 tig0012204:-30534 None:intergenic
GCAGCATGAAGCAGCAGAGG+AGG 0.582128 tig0012204:+28835 Msa1351370:CDS
CCTGTTTCTGAGATGAATGT+TGG 0.586683 tig0012204:-27074 None:intergenic
CAGCACGGAGTCCTCCGACT+GGG 0.587545 tig0012204:+30796 Msa1351370:CDS
GACACTGATTTCAGGAAACA+TGG 0.590046 tig0012204:+29763 Msa1351370:CDS
TCTTACCCATCTGAGAGGAA+AGG 0.590209 tig0012204:-27274 None:intergenic
CATAGCCTTTCCTCTCAGAT+GGG 0.597012 tig0012204:+27269 Msa1351370:exon
GGCCCCAACACAGTCTGCAA+AGG 0.604497 tig0012204:+30710 Msa1351370:CDS
AATGCGATGCATGCAAGCAT+TGG 0.605182 tig0012204:+29597 Msa1351370:CDS
TTGTCCAGCCATGACCTCCA+GGG 0.606858 tig0012204:-30956 None:intergenic
GGTATAGTATAATTATAAGG+AGG 0.607103 tig0012204:-31002 None:intergenic
TTGGCAGCATGAAGCAGCAG+AGG 0.610256 tig0012204:+28832 Msa1351370:CDS
AGTAATTGGATGAGCAGCAG+TGG 0.612150 tig0012204:-30850 None:intergenic
CCACCACACACCATACATGA+AGG 0.614977 tig0012204:+27040 Msa1351370:five_prime_UTR
TATGGAAGATGAGGATGGTG+AGG 0.620544 tig0012204:-26942 None:intergenic
CTTACTGGAGCTCGTCAGCA+CGG 0.621539 tig0012204:+30781 Msa1351370:CDS
TGTCCAGCCATGACCTCCAG+GGG 0.633773 tig0012204:-30955 None:intergenic
GAATCATAGCCCATCCCAGT+CGG 0.633968 tig0012204:-30810 None:intergenic
GTTGTTATGGAAGATGAGGA+TGG 0.634471 tig0012204:-26947 None:intergenic
ATTGTGTGTGGTCTAAACCT+TGG 0.638387 tig0012204:+31029 Msa1351370:three_prime_UTR
AAGGCCAAGATTAAGAGTCA+AGG 0.638913 tig0012204:+30729 Msa1351370:CDS
CCTTCATGTATGGTGTGTGG+TGG 0.639025 tig0012204:-27040 None:intergenic
CTATTACAAATACATGCCCA+AGG 0.639355 tig0012204:-31046 None:intergenic
ATGCATGCAAGCATTGGTAA+GGG 0.640516 tig0012204:+29603 Msa1351370:CDS
TCCTTTCTTACCCATCTGAG+AGG 0.647484 tig0012204:-27279 None:intergenic
CACCAACGACACCACAGTGG+AGG 0.650201 tig0012204:+30672 Msa1351370:CDS
AATTGTTGTTATGGAAGATG+AGG 0.653084 tig0012204:-26951 None:intergenic
TCATAGCCCATCCCAGTCGG+AGG 0.653601 tig0012204:-30807 None:intergenic
GGTCCTTCATGTATGGTGTG+TGG 0.670230 tig0012204:-27043 None:intergenic
AGATTGCAAGCACTTGTGAG+GGG 0.683402 tig0012204:+29544 Msa1351370:CDS
TTGTGAGGGGACATAATGTG+CGG 0.702353 tig0012204:+29557 Msa1351370:CDS
ACTATACCATGTATTGTGTG+TGG 0.706301 tig0012204:+31017 Msa1351370:three_prime_UTR
TGACTCACGACAAACACGAG+CGG 0.737315 tig0012204:+29659 Msa1351370:CDS
CACCACCAACGACACCACAG+TGG 0.740022 tig0012204:+30669 Msa1351370:CDS
GTCCAGCCATGACCTCCAGG+GGG 0.740945 tig0012204:-30954 None:intergenic
AGCTAGGTACACCTCCACTG+TGG 0.765897 tig0012204:-30683 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTTTAATTATTACTATTTA+TGG + tig0012204:31067-31086 Msa1351370:three_prime_UTR 10.0%
!!! ACAATCCTATTTAATTTTTA+AGG + tig0012204:27194-27213 Msa1351370:intron 15.0%
!!! ATTTGAGTTAATATTTCTTT+TGG + tig0012204:27532-27551 Msa1351370:intron 15.0%
!!! TTTGAGTTAATATTTCTTTT+GGG + tig0012204:27533-27552 Msa1351370:intron 15.0%
!!! TATGAGAAAAATCTATTTTT+TGG - tig0012204:27712-27731 None:intergenic 15.0%
!! ATATAATTACATACTGTATA+GGG - tig0012204:28026-28045 None:intergenic 15.0%
!! TATGTAATTATATGTGTATA+TGG + tig0012204:28033-28052 Msa1351370:intron 15.0%
!! AATATTTGTATTAGATTGTA+CGG + tig0012204:29359-29378 Msa1351370:intron 15.0%
!!! TCTATTCATTTTAATTAGAT+GGG - tig0012204:29890-29909 None:intergenic 15.0%
!!! TTTTTACAGACAAATATTAA+TGG - tig0012204:30161-30180 None:intergenic 15.0%
!! CAAAACTATATTATATATTG+TGG - tig0012204:30329-30348 None:intergenic 15.0%
!! TGTTTATGTATCTAAATTTG+AGG - tig0012204:27120-27139 None:intergenic 20.0%
!! GGAAACCTTAAAAATTAAAT+AGG - tig0012204:27202-27221 None:intergenic 20.0%
!! CAAAAAGAAAAAAGAAAGAA+AGG - tig0012204:27390-27409 None:intergenic 20.0%
!!! TCTTTTTGAAAACATTTATC+AGG + tig0012204:27402-27421 Msa1351370:intron 20.0%
!!! TTCAGTAAACAAACAAATTT+TGG - tig0012204:27458-27477 None:intergenic 20.0%
!!! TGACTAAATACTGATTATTA+TGG - tig0012204:27774-27793 None:intergenic 20.0%
!!! AATCAATTTATGCTTTCTTT+TGG - tig0012204:27841-27860 None:intergenic 20.0%
!! ATAAGTAATGCTACATATTA+TGG + tig0012204:27870-27889 Msa1351370:intron 20.0%
!! CATATAATTACATACTGTAT+AGG - tig0012204:28027-28046 None:intergenic 20.0%
!! ACTCATAATTAACAATCATT+TGG - tig0012204:28251-28270 None:intergenic 20.0%
!! TTTGTTAAATACAAGAGAAT+TGG + tig0012204:29863-29882 Msa1351370:intron 20.0%
!! TTGTTAAATACAAGAGAATT+GGG + tig0012204:29864-29883 Msa1351370:intron 20.0%
!!! CTCTATTCATTTTAATTAGA+TGG - tig0012204:29891-29910 None:intergenic 20.0%
!! ATAAAACAAGTAATATGAGT+TGG - tig0012204:29975-29994 None:intergenic 20.0%
!!! TATTAATGGTCAATTTGTTA+GGG - tig0012204:30147-30166 None:intergenic 20.0%
!!! ATATTAATGGTCAATTTGTT+AGG - tig0012204:30148-30167 None:intergenic 20.0%
!! CATGGTATAGTATAATTATA+AGG - tig0012204:31008-31027 None:intergenic 20.0%
!!! ATGCACATTTTATTAATCAA+CGG - tig0012204:31255-31274 None:intergenic 20.0%
!! TGAAATCCTAATTGTTGTTA+TGG - tig0012204:26963-26982 None:intergenic 25.0%
! GGAAGAAAAATAACATTGAT+TGG - tig0012204:27429-27448 None:intergenic 25.0%
!!! ACAAACAAATTTTGGTGAAT+GGG - tig0012204:27450-27469 None:intergenic 25.0%
!!! AACAAACAAATTTTGGTGAA+TGG - tig0012204:27451-27470 None:intergenic 25.0%
!!! TTTTTGAGACTTGATCAAAA+TGG - tig0012204:27491-27510 None:intergenic 25.0%
! ATGTTATCCTTTATTGTGAA+AGG - tig0012204:27903-27922 None:intergenic 25.0%
! CATTTACTGTACTTATATGT+AGG - tig0012204:27928-27947 None:intergenic 25.0%
! TGATTGTTAATTATGAGTGA+AGG + tig0012204:28253-28272 Msa1351370:intron 25.0%
! ATTATGAGTGAAGGATTATT+TGG + tig0012204:28262-28281 Msa1351370:intron 25.0%
! TGAAATAAATTGTGTTCGTT+CGG + tig0012204:28464-28483 Msa1351370:intron 25.0%
! AACAATGACTTAATCTATGT+TGG + tig0012204:28774-28793 Msa1351370:intron 25.0%
! ACAATGACTTAATCTATGTT+GGG + tig0012204:28775-28794 Msa1351370:intron 25.0%
!! AAGTCTTCTTTTATCTGTTT+TGG + tig0012204:29108-29127 Msa1351370:intron 25.0%
! ATGAGTGTATGACATATTTA+TGG - tig0012204:29204-29223 None:intergenic 25.0%
! AATCAATGTGACTTTAATGT+TGG - tig0012204:29306-29325 None:intergenic 25.0%
!! AATACAAATATTTTCCATGC+AGG - tig0012204:29351-29370 None:intergenic 25.0%
! CTAAGAAAAAATGAATAACC+TGG - tig0012204:29834-29853 None:intergenic 25.0%
!!! ATTAATGGTCAATTTGTTAG+GGG - tig0012204:30146-30165 None:intergenic 25.0%
! CAAGCATCAAATATGTTAAA+AGG - tig0012204:30217-30236 None:intergenic 25.0%
!! CTTTTAACATATTTGATGCT+TGG + tig0012204:30215-30234 Msa1351370:intron 25.0%
! TTTCACTATCATCAACATTT+GGG - tig0012204:30404-30423 None:intergenic 25.0%
! TATCACAATCAAACAATGTT+AGG + tig0012204:30484-30503 Msa1351370:CDS 25.0%
! GACTTAAATCATGAAATTCT+TGG - tig0012204:30638-30657 None:intergenic 25.0%
! GGTATAGTATAATTATAAGG+AGG - tig0012204:31005-31024 None:intergenic 25.0%
!! GAATCATAATATTCGTTTTC+GGG - tig0012204:31138-31157 None:intergenic 25.0%
!! GGAATCATAATATTCGTTTT+CGG - tig0012204:31139-31158 None:intergenic 25.0%
!! TGCACATTTTATTAATCAAC+GGG - tig0012204:31254-31273 None:intergenic 25.0%
! AATTGTTGTTATGGAAGATG+AGG - tig0012204:26954-26973 None:intergenic 30.0%
CATCTTCCATAACAACAATT+AGG + tig0012204:26954-26973 Msa1351370:five_prime_UTR 30.0%
CATTCTCTTCGTAACATATA+AGG + tig0012204:27138-27157 Msa1351370:intron 30.0%
!!! AGTTTTCAAGTCTTCTTCTA+AGG + tig0012204:27328-27347 Msa1351370:CDS 30.0%
TCTCATAGACAATAAGAATC+TGG + tig0012204:27725-27744 Msa1351370:intron 30.0%
!!! TATGCTTTCTTTTGGTTTCT+TGG - tig0012204:27833-27852 None:intergenic 30.0%
! GTTGTAGTTGTTGTCTTTTA+TGG + tig0012204:27957-27976 Msa1351370:intron 30.0%
! TCTTTTATGGAACCACATAA+AGG + tig0012204:27970-27989 Msa1351370:intron 30.0%
ATGTTAAGTTCACATGATAG+GGG - tig0012204:28060-28079 None:intergenic 30.0%
CATGTTAAGTTCACATGATA+GGG - tig0012204:28061-28080 None:intergenic 30.0%
ACATGTTAAGTTCACATGAT+AGG - tig0012204:28062-28081 None:intergenic 30.0%
CACTATGGAAATCTAACATT+GGG - tig0012204:28174-28193 None:intergenic 30.0%
TAATTAAGTGATTAACCAGC+TGG - tig0012204:28211-28230 None:intergenic 30.0%
! TTTTCACTTTCAGAAGGAAA+AGG + tig0012204:28802-28821 Msa1351370:intron 30.0%
CAGATAAAAGAAGACTTACT+AGG - tig0012204:29105-29124 None:intergenic 30.0%
ATTAATTCTGTGGTTCAGTA+TGG + tig0012204:29177-29196 Msa1351370:intron 30.0%
GTAACTGAAAGTTTGAAAGT+AGG - tig0012204:29228-29247 None:intergenic 30.0%
TGACACTTGCAATATATGAT+CGG + tig0012204:29268-29287 Msa1351370:intron 30.0%
TAATTGATTGAGTGTGTTCA+GGG - tig0012204:29401-29420 None:intergenic 30.0%
TTAATTGATTGAGTGTGTTC+AGG - tig0012204:29402-29421 None:intergenic 30.0%
AGCTAATGACAGAAAAACAT+AGG - tig0012204:29448-29467 None:intergenic 30.0%
TCAAAGTAGCCAGAAAATTA+AGG + tig0012204:29741-29760 Msa1351370:CDS 30.0%
! AATCAGTGTCCTTAATTTTC+TGG - tig0012204:29753-29772 None:intergenic 30.0%
!! AAATTAAGGACACTGATTTC+AGG + tig0012204:29755-29774 Msa1351370:CDS 30.0%
AAAATGAATAGAGTGTAGAG+TGG + tig0012204:29898-29917 Msa1351370:intron 30.0%
AAGTTAGTTGCTCAATTTAG+AGG + tig0012204:30001-30020 Msa1351370:intron 30.0%
!! TTAATGGTCAATTTGTTAGG+GGG - tig0012204:30145-30164 None:intergenic 30.0%
AATGATTCTTCAGATGGAAT+TGG - tig0012204:30305-30324 None:intergenic 30.0%
CTTTCACTATCATCAACATT+TGG - tig0012204:30405-30424 None:intergenic 30.0%
TGAAGAAAAAACAGTTGACA+TGG + tig0012204:30563-30582 Msa1351370:CDS 30.0%
AAAAACAGTTGACATGGATA+TGG + tig0012204:30569-30588 Msa1351370:CDS 30.0%
ATTCATGGGACAGTAATGTT+TGG - tig0012204:27010-27029 None:intergenic 35.0%
!!! GTGGCATTTTTGAGATTCAT+GGG - tig0012204:27024-27043 None:intergenic 35.0%
!! AGATGAATGTTGGTAGTTAC+TGG - tig0012204:27067-27086 None:intergenic 35.0%
! GGATTGTTACCTTGTCTTTT+AGG - tig0012204:27181-27200 None:intergenic 35.0%
CAATTCAGACAACATGTTAG+AGG - tig0012204:27223-27242 None:intergenic 35.0%
TGTAGCATTACTTATACTGC+AGG - tig0012204:27865-27884 None:intergenic 35.0%
CAGTGTACCTTTCACAATAA+AGG + tig0012204:27893-27912 Msa1351370:intron 35.0%
AGGTAGATTCATCCTTTATG+TGG - tig0012204:27985-28004 None:intergenic 35.0%
GATGAATGATGTGATGTGTT+AGG - tig0012204:28134-28153 None:intergenic 35.0%
GCACTATGGAAATCTAACAT+TGG - tig0012204:28175-28194 None:intergenic 35.0%
! GATGGATTTGTTTAGCACTA+TGG - tig0012204:28189-28208 None:intergenic 35.0%
GAGTGAAGGATTATTTGGAT+CGG + tig0012204:28267-28286 Msa1351370:intron 35.0%
TAAACACTGATGAGACTGTT+TGG + tig0012204:28314-28333 Msa1351370:intron 35.0%
AAACACTGATGAGACTGTTT+GGG + tig0012204:28315-28334 Msa1351370:intron 35.0%
CATATAGTCGTCTATTTGGA+TGG - tig0012204:28731-28750 None:intergenic 35.0%
AGTGCATATAGTCGTCTATT+TGG - tig0012204:28735-28754 None:intergenic 35.0%
!!! GGACTTTTTTCACTTTCAGA+AGG + tig0012204:28796-28815 Msa1351370:intron 35.0%
AGAAGGAAAAGGAAGAGAAT+TGG + tig0012204:28813-28832 Msa1351370:intron 35.0%
ATATCTGGTGAACTATATGC+AGG - tig0012204:28878-28897 None:intergenic 35.0%
TGATTTCTCTCTTCAGTCAA+GGG - tig0012204:28929-28948 None:intergenic 35.0%
ATGATTTCTCTCTTCAGTCA+AGG - tig0012204:28930-28949 None:intergenic 35.0%
TACTAGGTATCCTCTATAGT+AGG - tig0012204:29089-29108 None:intergenic 35.0%
GCAGCATCATATTAATTCTG+TGG + tig0012204:29167-29186 Msa1351370:intron 35.0%
ATCATTGTAGTAAACCTGCA+TGG + tig0012204:29334-29353 Msa1351370:intron 35.0%
AGCTGTTCCATTTCGTATAT+AGG + tig0012204:29486-29505 Msa1351370:intron 35.0%
TTTGTGTCTAACTTTGTCGT+AGG - tig0012204:29703-29722 None:intergenic 35.0%
ACGACAAAGTTAGACACAAA+TGG + tig0012204:29703-29722 Msa1351370:CDS 35.0%
CAAAGTTAGACACAAATGGT+TGG + tig0012204:29707-29726 Msa1351370:CDS 35.0%
ACACAAATGGTTGGAACTAT+AGG + tig0012204:29716-29735 Msa1351370:CDS 35.0%
ACATGGAGCTACAATGAATA+AGG + tig0012204:29780-29799 Msa1351370:CDS 35.0%
!! CTAAGGAAGTATTGTGAAGT+AGG + tig0012204:30088-30107 Msa1351370:intron 35.0%
!! TAATGGTCAATTTGTTAGGG+GGG - tig0012204:30144-30163 None:intergenic 35.0%
! TGTGGTAATGATTCTTCAGA+TGG - tig0012204:30311-30330 None:intergenic 35.0%
TCAAACAATGTTAGGCCTTT+AGG + tig0012204:30492-30511 Msa1351370:CDS 35.0%
CAAACAATGTTAGGCCTTTA+GGG + tig0012204:30493-30512 Msa1351370:CDS 35.0%
ATATCATCAGTGGGTGTATA+TGG - tig0012204:30540-30559 None:intergenic 35.0%
TCTTCAGACATATCATCAGT+GGG - tig0012204:30549-30568 None:intergenic 35.0%
TTCTTCAGACATATCATCAG+TGG - tig0012204:30550-30569 None:intergenic 35.0%
CATGAAATTCTTGGTTCATG+AGG - tig0012204:30629-30648 None:intergenic 35.0%
!! TCCCATTAATTTTTGGGCTT+GGG - tig0012204:30881-30900 None:intergenic 35.0%
!! ATCCCATTAATTTTTGGGCT+TGG - tig0012204:30882-30901 None:intergenic 35.0%
! GGCGAATCCCATTAATTTTT+GGG - tig0012204:30887-30906 None:intergenic 35.0%
! AGGCGAATCCCATTAATTTT+TGG - tig0012204:30888-30907 None:intergenic 35.0%
! CCTTCAATCTAAACGGATTT+CGG + tig0012204:30905-30924 Msa1351370:CDS 35.0%
! CTTCAATCTAAACGGATTTC+GGG + tig0012204:30906-30925 Msa1351370:CDS 35.0%
ACTATACCATGTATTGTGTG+TGG + tig0012204:31017-31036 Msa1351370:three_prime_UTR 35.0%
TTTAGACCACACACAATACA+TGG - tig0012204:31026-31045 None:intergenic 35.0%
CTATTACAAATACATGCCCA+AGG - tig0012204:31049-31068 None:intergenic 35.0%
TGACAGTGACACAAAACATA+TGG - tig0012204:31160-31179 None:intergenic 35.0%
GTTGTTATGGAAGATGAGGA+TGG - tig0012204:26950-26969 None:intergenic 40.0%
!!! GGTGGCATTTTTGAGATTCA+TGG - tig0012204:27025-27044 None:intergenic 40.0%
AGTTACTGGTCCTTCATGTA+TGG - tig0012204:27053-27072 None:intergenic 40.0%
!! CCTGTTTCTGAGATGAATGT+TGG - tig0012204:27077-27096 None:intergenic 40.0%
CCAACATTCATCTCAGAAAC+AGG + tig0012204:27074-27093 Msa1351370:five_prime_UTR 40.0%
CGTGTATGTCCTAAAAGACA+AGG + tig0012204:27169-27188 Msa1351370:intron 40.0%
CTCAACTCTACAAGAGAACA+AGG + tig0012204:27352-27371 Msa1351370:CDS 40.0%
GATGAATCTACCTTGCCTTT+AGG + tig0012204:27992-28011 Msa1351370:intron 40.0%
TACATACTGTATAGGGTTGC+TGG - tig0012204:28019-28038 None:intergenic 40.0%
TAAGTGATTAACCAGCTGGA+TGG - tig0012204:28207-28226 None:intergenic 40.0%
! GACTAATTCCATTAGCTCTC+TGG + tig0012204:28376-28395 Msa1351370:intron 40.0%
TAAGCTATCCAGAGAGCTAA+TGG - tig0012204:28387-28406 None:intergenic 40.0%
TCACCAGATATCACGAATGA+AGG + tig0012204:28887-28906 Msa1351370:CDS 40.0%
CTTCCTTCATTCGTGATATC+TGG - tig0012204:28893-28912 None:intergenic 40.0%
AGGTCATGGACATAACTCTA+AGG + tig0012204:29036-29055 Msa1351370:CDS 40.0%
CTCATTCAGTCCTACTATAG+AGG + tig0012204:29076-29095 Msa1351370:CDS 40.0%
!! ATGCATGCAAGCATTGGTAA+GGG + tig0012204:29603-29622 Msa1351370:CDS 40.0%
!! GACACTGATTTCAGGAAACA+TGG + tig0012204:29763-29782 Msa1351370:CDS 40.0%
! GCTTGGTGACTACACAAAAA+CGG + tig0012204:30232-30251 Msa1351370:intron 40.0%
GCATAGAAAACCACATGCAA+AGG + tig0012204:30271-30290 Msa1351370:intron 40.0%
! GGAATTGATTCCTTTGCATG+TGG - tig0012204:30284-30303 None:intergenic 40.0%
CAACCAGTTCCAATCCAATT+GGG - tig0012204:30451-30470 None:intergenic 40.0%
!! AATGTTAGGCCTTTAGGGTT+GGG + tig0012204:30498-30517 Msa1351370:CDS 40.0%
! TTTGCATGGATTGAGTCTCT+TGG - tig0012204:30600-30619 None:intergenic 40.0%
AGACTCAATCCATGCAAACA+TGG + tig0012204:30602-30621 Msa1351370:CDS 40.0%
GACTCAATCCATGCAAACAT+GGG + tig0012204:30603-30622 Msa1351370:CDS 40.0%
AAGGCCAAGATTAAGAGTCA+AGG + tig0012204:30729-30748 Msa1351370:CDS 40.0%
AGCCCAAGCCCAAAAATTAA+TGG + tig0012204:30876-30895 Msa1351370:CDS 40.0%
GCCCAAGCCCAAAAATTAAT+GGG + tig0012204:30877-30896 Msa1351370:CDS 40.0%
GGATTCGCCTTCAATCTAAA+CGG + tig0012204:30898-30917 Msa1351370:CDS 40.0%
CCGAAATCCGTTTAGATTGA+AGG - tig0012204:30908-30927 None:intergenic 40.0%
ATTGTGTGTGGTCTAAACCT+TGG + tig0012204:31029-31048 Msa1351370:three_prime_UTR 40.0%
TTGTGTGTGGTCTAAACCTT+GGG + tig0012204:31030-31049 Msa1351370:three_prime_UTR 40.0%
!!! ATTCGTTTTCGGGTGTCTTT+TGG - tig0012204:31128-31147 None:intergenic 40.0%
TATGGAAGATGAGGATGGTG+AGG - tig0012204:26945-26964 None:intergenic 45.0%
ACATAGCCTTTCCTCTCAGA+TGG + tig0012204:27268-27287 Msa1351370:exon 45.0%
CATAGCCTTTCCTCTCAGAT+GGG + tig0012204:27269-27288 Msa1351370:exon 45.0%
TCTTACCCATCTGAGAGGAA+AGG - tig0012204:27277-27296 None:intergenic 45.0%
TCCTCTCAGATGGGTAAGAA+AGG + tig0012204:27278-27297 Msa1351370:exon 45.0%
TCCTTTCTTACCCATCTGAG+AGG - tig0012204:27282-27301 None:intergenic 45.0%
TGGGTAAGAAAGGAAGCAGT+TGG + tig0012204:27288-27307 Msa1351370:CDS 45.0%
TATAGGGTTGCTGGTCCTAA+AGG - tig0012204:28010-28029 None:intergenic 45.0%
GCTAAACAAATCCATCCAGC+TGG + tig0012204:28193-28212 Msa1351370:intron 45.0%
!! TTCAGTCAAGGGTGTTGATG+TGG - tig0012204:28918-28937 None:intergenic 45.0%
AGCTGCTAGAGTTGTGAGAT+TGG + tig0012204:29012-29031 Msa1351370:CDS 45.0%
CGTCGAGCCTATATACGAAA+TGG - tig0012204:29496-29515 None:intergenic 45.0%
TGAGATTGCAAGCACTTGTG+AGG + tig0012204:29542-29561 Msa1351370:CDS 45.0%
GAGATTGCAAGCACTTGTGA+GGG + tig0012204:29543-29562 Msa1351370:CDS 45.0%
AGATTGCAAGCACTTGTGAG+GGG + tig0012204:29544-29563 Msa1351370:CDS 45.0%
TTGTGAGGGGACATAATGTG+CGG + tig0012204:29557-29576 Msa1351370:CDS 45.0%
AATGCGATGCATGCAAGCAT+TGG + tig0012204:29597-29616 Msa1351370:CDS 45.0%
!! GATGCATGCAAGCATTGGTA+AGG + tig0012204:29602-29621 Msa1351370:CDS 45.0%
CGTGTTTGTCGTGAGTCAAT+TGG - tig0012204:29657-29676 None:intergenic 45.0%
ACCTTTCATGTAGATCCCGA+TGG + tig0012204:30037-30056 Msa1351370:intron 45.0%
ACCATCGGGATCTACATGAA+AGG - tig0012204:30041-30060 None:intergenic 45.0%
GACTACATCAGTACACCATC+GGG - tig0012204:30055-30074 None:intergenic 45.0%
CGACTACATCAGTACACCAT+CGG - tig0012204:30056-30075 None:intergenic 45.0%
! CGATGGTGTACTGATGTAGT+CGG + tig0012204:30054-30073 Msa1351370:intron 45.0%
TACTGATGTAGTCGGTCGTT+AGG + tig0012204:30062-30081 Msa1351370:intron 45.0%
GTGAAAGAGCCCAATTGGAT+TGG + tig0012204:30439-30458 Msa1351370:CDS 45.0%
CCAACCAGTTCCAATCCAAT+TGG - tig0012204:30452-30471 None:intergenic 45.0%
! CCAATTGGATTGGAACTGGT+TGG + tig0012204:30449-30468 Msa1351370:CDS 45.0%
!! CAATGTTAGGCCTTTAGGGT+TGG + tig0012204:30497-30516 Msa1351370:CDS 45.0%
!! ATGTTAGGCCTTTAGGGTTG+GGG + tig0012204:30499-30518 Msa1351370:CDS 45.0%
ATCAGTGGGTGTATATGGTG+GGG - tig0012204:30535-30554 None:intergenic 45.0%
CATCAGTGGGTGTATATGGT+GGG - tig0012204:30536-30555 None:intergenic 45.0%
TCATCAGTGGGTGTATATGG+TGG - tig0012204:30537-30556 None:intergenic 45.0%
!! CGTTGGTGGTGTTTAGAGAT+AGG - tig0012204:30660-30679 None:intergenic 45.0%
! TGGAGGTGTACCTAGCTATA+TGG + tig0012204:30689-30708 Msa1351370:CDS 45.0%
TGGGCCTTGACTCTTAATCT+TGG - tig0012204:30736-30755 None:intergenic 45.0%
!! TATGATTCATCTGGCTCTGG+TGG + tig0012204:30825-30844 Msa1351370:CDS 45.0%
!! ATGATTCATCTGGCTCTGGT+GGG + tig0012204:30826-30845 Msa1351370:CDS 45.0%
AGTAATTGGATGAGCAGCAG+TGG - tig0012204:30853-30872 None:intergenic 45.0%
!! TTTCGGGTGTCTTTTGGTGT+GGG - tig0012204:31122-31141 None:intergenic 45.0%
!!! TTTTCGGGTGTCTTTTGGTG+TGG - tig0012204:31123-31142 None:intergenic 45.0%
CCTTCATGTATGGTGTGTGG+TGG - tig0012204:27043-27062 None:intergenic 50.0%
CCACCACACACCATACATGA+AGG + tig0012204:27040-27059 Msa1351370:five_prime_UTR 50.0%
GGTCCTTCATGTATGGTGTG+TGG - tig0012204:27046-27065 None:intergenic 50.0%
CTGCGAGAGCTTACGGAAAT+GGG - tig0012204:28667-28686 None:intergenic 50.0%
TCTGCGAGAGCTTACGGAAA+TGG - tig0012204:28668-28687 None:intergenic 50.0%
AGACTGTCTGCGAGAGCTTA+CGG - tig0012204:28674-28693 None:intergenic 50.0%
!! AGCAGCAGAGGAGGTATCTA+TGG + tig0012204:28844-28863 Msa1351370:CDS 50.0%
GCTAGAGTTGTGAGATTGGC+AGG + tig0012204:29016-29035 Msa1351370:CDS 50.0%
GTTGTGAGATTGGCAGGTCA+TGG + tig0012204:29022-29041 Msa1351370:CDS 50.0%
!! GCAAGCATTGGTAAGGGTTC+AGG + tig0012204:29609-29628 Msa1351370:CDS 50.0%
TGACTCACGACAAACACGAG+CGG + tig0012204:29659-29678 Msa1351370:CDS 50.0%
TCCATACGCTTTCAACTGCC+AGG + tig0012204:29813-29832 Msa1351370:CDS 50.0%
! ACCTGGCAGTTGAAAGCGTA+TGG - tig0012204:29817-29836 None:intergenic 50.0%
AGTCGGTCGTTAGGATGCTA+AGG + tig0012204:30071-30090 Msa1351370:intron 50.0%
!! TGGTCAATTTGTTAGGGGGG+TGG - tig0012204:30141-30160 None:intergenic 50.0%
TGAGCGTGAAAGAGCCCAAT+TGG + tig0012204:30434-30453 Msa1351370:CDS 50.0%
! GAGCCCAATTGGATTGGAAC+TGG + tig0012204:30445-30464 Msa1351370:CDS 50.0%
ATTGGAACTGGTTGGAGCGT+TGG + tig0012204:30457-30476 Msa1351370:CDS 50.0%
! GTATATGGTGGGGTCTCAAG+TGG - tig0012204:30525-30544 None:intergenic 50.0%
TCATGAGGCCCATGTTTGCA+TGG - tig0012204:30614-30633 None:intergenic 50.0%
TGTGTTGGGGCCATATAGCT+AGG - tig0012204:30702-30721 None:intergenic 50.0%
TGGCCTTTGCAGACTGTGTT+GGG - tig0012204:30716-30735 None:intergenic 50.0%
! TTGGCCTTTGCAGACTGTGT+TGG - tig0012204:30717-30736 None:intergenic 50.0%
GTCAAGGCCCATCAAAGCAT+CGG + tig0012204:30745-30764 Msa1351370:CDS 50.0%
! TCAAGGCCCATCAAAGCATC+GGG + tig0012204:30746-30765 Msa1351370:CDS 50.0%
GAATCATAGCCCATCCCAGT+CGG - tig0012204:30813-30832 None:intergenic 50.0%
!! GGGATGGGCTATGATTCATC+TGG + tig0012204:30816-30835 Msa1351370:CDS 50.0%
! GGCTATGATTCATCTGGCTC+TGG + tig0012204:30822-30841 Msa1351370:CDS 50.0%
!! TGATTCATCTGGCTCTGGTG+GGG + tig0012204:30827-30846 Msa1351370:CDS 50.0%
GGGCTTGGGCTTCTAGTAAT+TGG - tig0012204:30867-30886 None:intergenic 50.0%
! TCTAAACGGATTTCGGGTGC+TGG + tig0012204:30912-30931 Msa1351370:CDS 50.0%
!! TTCGGGTGTCTTTTGGTGTG+GGG - tig0012204:31121-31140 None:intergenic 50.0%
GGTTGCTGGTCCTAAAGGCA+AGG - tig0012204:28005-28024 None:intergenic 55.0%
TTGGCAGCATGAAGCAGCAG+AGG + tig0012204:28832-28851 Msa1351370:CDS 55.0%
TGCTGCTGTTGCTGCAGCAT+AGG - tig0012204:28960-28979 None:intergenic 55.0%
ACGTGCACTGCGTGCTTTGA+AGG + tig0012204:29513-29532 Msa1351370:CDS 55.0%
! CGTGCACTGCGTGCTTTGAA+GGG + tig0012204:29514-29533 Msa1351370:CDS 55.0%
CAAACACGAGCGGACAGTAG+AGG + tig0012204:29669-29688 Msa1351370:CDS 55.0%
TCAAGTGGCCCCAACCCTAA+AGG - tig0012204:30510-30529 None:intergenic 55.0%
AGCTAGGTACACCTCCACTG+TGG - tig0012204:30686-30705 None:intergenic 55.0%
GGCCTTTGCAGACTGTGTTG+GGG - tig0012204:30715-30734 None:intergenic 55.0%
ACGACGCCCGATGCTTTGAT+GGG - tig0012204:30755-30774 None:intergenic 55.0%
AACGACGCCCGATGCTTTGA+TGG - tig0012204:30756-30775 None:intergenic 55.0%
!! TCAAAGCATCGGGCGTCGTT+TGG + tig0012204:30756-30775 Msa1351370:CDS 55.0%
! GGGCGTCGTTTGGATCTTAC+TGG + tig0012204:30766-30785 Msa1351370:CDS 55.0%
CTTACTGGAGCTCGTCAGCA+CGG + tig0012204:30781-30800 Msa1351370:CDS 55.0%
!! GATTTCGGGTGCTGGTGTTG+AGG + tig0012204:30920-30939 Msa1351370:CDS 55.0%
TTGTCCAGCCATGACCTCCA+GGG - tig0012204:30959-30978 None:intergenic 55.0%
GCAGCATGAAGCAGCAGAGG+AGG + tig0012204:28835-28854 Msa1351370:CDS 60.0%
AGCAGCAGCTGAAGCTGCAG+TGG + tig0012204:28976-28995 Msa1351370:CDS 60.0%
AGCTGAAGCTGCAGTGGCTG+CGG + tig0012204:28982-29001 Msa1351370:CDS 60.0%
CACCACCAACGACACCACAG+TGG + tig0012204:30669-30688 Msa1351370:CDS 60.0%
!! CTCCACTGTGGTGTCGTTGG+TGG - tig0012204:30674-30693 None:intergenic 60.0%
CACCAACGACACCACAGTGG+AGG + tig0012204:30672-30691 Msa1351370:CDS 60.0%
!! CACCTCCACTGTGGTGTCGT+TGG - tig0012204:30677-30696 None:intergenic 60.0%
GGCCCCAACACAGTCTGCAA+AGG + tig0012204:30710-30729 Msa1351370:CDS 60.0%
TCATAGCCCATCCCAGTCGG+AGG - tig0012204:30810-30829 None:intergenic 60.0%
!! CGGGTGCTGGTGTTGAGGAT+TGG + tig0012204:30925-30944 Msa1351370:CDS 60.0%
!! GAGGATTGGCCAATTCCCCC+TGG + tig0012204:30939-30958 Msa1351370:CDS 60.0%
! GATTGGCCAATTCCCCCTGG+AGG + tig0012204:30942-30961 Msa1351370:CDS 60.0%
CCATGACCTCCAGGGGGAAT+TGG - tig0012204:30951-30970 None:intergenic 60.0%
CCAATTCCCCCTGGAGGTCA+TGG + tig0012204:30948-30967 Msa1351370:CDS 60.0%
TGTCCAGCCATGACCTCCAG+GGG - tig0012204:30958-30977 None:intergenic 60.0%
CTTGTCCAGCCATGACCTCC+AGG - tig0012204:30960-30979 None:intergenic 60.0%
TCAGCACGGAGTCCTCCGAC+TGG + tig0012204:30795-30814 Msa1351370:CDS 65.0%
CAGCACGGAGTCCTCCGACT+GGG + tig0012204:30796-30815 Msa1351370:CDS 65.0%
ACGGAGTCCTCCGACTGGGA+TGG + tig0012204:30800-30819 Msa1351370:CDS 65.0%
CGGAGTCCTCCGACTGGGAT+GGG + tig0012204:30801-30820 Msa1351370:CDS 65.0%
TTCCCCCTGGAGGTCATGGC+TGG + tig0012204:30952-30971 Msa1351370:CDS 65.0%
GTCCAGCCATGACCTCCAGG+GGG - tig0012204:30957-30976 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0012204 gene 26891 31280 26891 ID=Msa1351370;Name=Msa1351370
tig0012204 mRNA 26891 31280 26891 ID=Msa1351370-mRNA-1;Parent=Msa1351370;Name=Msa1351370-mRNA-1;_AED=0.02;_eAED=0.02;_QI=226|1|1|1|0.75|0.6|5|291|440
tig0012204 exon 26891 27095 26891 ID=Msa1351370-mRNA-1:exon:7708;Parent=Msa1351370-mRNA-1
tig0012204 exon 27266 27373 27266 ID=Msa1351370-mRNA-1:exon:7709;Parent=Msa1351370-mRNA-1
tig0012204 exon 28815 29105 28815 ID=Msa1351370-mRNA-1:exon:7710;Parent=Msa1351370-mRNA-1
tig0012204 exon 29508 29834 29508 ID=Msa1351370-mRNA-1:exon:7711;Parent=Msa1351370-mRNA-1
tig0012204 exon 30372 31280 30372 ID=Msa1351370-mRNA-1:exon:7712;Parent=Msa1351370-mRNA-1
tig0012204 five_prime_UTR 26891 27095 26891 ID=Msa1351370-mRNA-1:five_prime_utr;Parent=Msa1351370-mRNA-1
tig0012204 five_prime_UTR 27266 27286 27266 ID=Msa1351370-mRNA-1:five_prime_utr;Parent=Msa1351370-mRNA-1
tig0012204 CDS 27287 27373 27287 ID=Msa1351370-mRNA-1:cds;Parent=Msa1351370-mRNA-1
tig0012204 CDS 28815 29105 28815 ID=Msa1351370-mRNA-1:cds;Parent=Msa1351370-mRNA-1
tig0012204 CDS 29508 29834 29508 ID=Msa1351370-mRNA-1:cds;Parent=Msa1351370-mRNA-1
tig0012204 CDS 30372 30989 30372 ID=Msa1351370-mRNA-1:cds;Parent=Msa1351370-mRNA-1
tig0012204 three_prime_UTR 30990 31280 30990 ID=Msa1351370-mRNA-1:three_prime_utr;Parent=Msa1351370-mRNA-1
Gene Sequence

>Msa1351370

ATGGGTAAGAAAGGAAGCAGTTGGTTCTCATCTGTGAAGAAAGTTTTCAAGTCTTCTTCTAAGGACTCAACTCTACAAGAGAACAAGAAGGAAAAGGAAGAGAATTGGCAGCATGAAGCAGCAGAGGAGGTATCTATGGAGCATTTTCCTGCATATAGTTCACCAGATATCACGAATGAAGGAAGCACCACATCAACACCCTTGACTGAAGAGAGAAATCATGCAATTGCCTATGCTGCAGCAACAGCAGCAGCAGCTGAAGCTGCAGTGGCTGCGGCTCAAGCAGCTGCTAGAGTTGTGAGATTGGCAGGTCATGGACATAACTCTAAGGTTGAAAAAGCAGCAATACTCATTCAGTCCTACTATAGAGGATACCTAGCTCGACGTGCACTGCGTGCTTTGAAGGGATTAGTGAGATTGCAAGCACTTGTGAGGGGACATAATGTGCGGAAGCAAGCACAGATGACAATGCGATGCATGCAAGCATTGGTAAGGGTTCAGGCAAGAGTAAGAGCTCGCAGACTCCAATTGACTCACGACAAACACGAGCGGACAGTAGAGGAACAACATCCTACGACAAAGTTAGACACAAATGGTTGGAACTATAGGCGTCAAAGTAGCCAGAAAATTAAGGACACTGATTTCAGGAAACATGGAGCTACAATGAATAAGGAGAAAGCTCTTCCATACGCTTTCAACTGCCAGCAACTGCAGAAGCAATATTTGCACATAGACCCAAATGTTGATGATAGTGAAAGTTACAGCAATGAGCGTGAAAGAGCCCAATTGGATTGGAACTGGTTGGAGCGTTGGATGTTATCACAATCAAACAATGTTAGGCCTTTAGGGTTGGGGCCACTTGAGACCCCACCATATACACCCACTGATGATATGTCTGAAGAAAAAACAGTTGACATGGATATGGTTGCACCAAGAGACTCAATCCATGCAAACATGGGCCTCATGAACCAAGAATTTCATGATTTAAGTCCTATCTCTAAACACCACCAACGACACCACAGTGGAGGTGTACCTAGCTATATGGCCCCAACACAGTCTGCAAAGGCCAAGATTAAGAGTCAAGGCCCATCAAAGCATCGGGCGTCGTTTGGATCTTACTGGAGCTCGTCAGCACGGAGTCCTCCGACTGGGATGGGCTATGATTCATCTGGCTCTGGTGGGGCCACTGCTGCTCATCCAATTACTAGAAGCCCAAGCCCAAAAATTAATGGGATTCGCCTTCAATCTAAACGGATTTCGGGTGCTGGTGTTGAGGATTGGCCAATTCCCCCTGGAGGTCATGGCTGGACAAGATTTGATTGA
Protein sequence

>Msa1351370

MGKKGSSWFSSVKKVFKSSSKDSTLQENKKEKEENWQHEAAEEVSMEHFPAYSSPDITNEGSTTSTPLTEERNHAIAYAAATAAAAEAAVAAAQAAARVVRLAGHGHNSKVEKAAILIQSYYRGYLARRALRALKGLVRLQALVRGHNVRKQAQMTMRCMQALVRVQARVRARRLQLTHDKHERTVEEQHPTTKLDTNGWNYRRQSSQKIKDTDFRKHGATMNKEKALPYAFNCQQLQKQYLHIDPNVDDSESYSNERERAQLDWNWLERWMLSQSNNVRPLGLGPLETPPYTPTDDMSEEKTVDMDMVAPRDSIHANMGLMNQEFHDLSPISKHHQRHHSGGVPSYMAPTQSAKAKIKSQGPSKHRASFGSYWSSSARSPPTGMGYDSSGSGGATAAHPITRSPSPKINGIRLQSKRISGAGVEDWPIPPGGHGWTRFD*