AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0218040

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0218040 MtrunA17_Chr2g0320061 97.484 318 8 0 1 318 1 318 0.0 632
Msa0218040 MtrunA17_Chr2g0320051 92.453 318 24 0 1 318 1 318 0.0 601
Msa0218040 MtrunA17_Chr2g0320031 83.072 319 53 1 1 318 1 319 0.0 548
Msa0218040 MtrunA17_Chr2g0320101 83.962 318 50 1 1 318 1 317 0.0 545
Msa0218040 MtrunA17_Chr2g0320071 83.648 318 51 1 1 318 1 317 0.0 540
Msa0218040 MtrunA17_Chr2g0320091 77.116 319 71 2 1 318 1 318 2.37e-178 495
Msa0218040 MtrunA17_Chr3g0129051 66.254 323 103 4 1 318 1 322 1.04e-146 416
Msa0218040 MtrunA17_Chr3g0129061 65.944 323 104 4 1 318 1 322 1.12e-141 402
Msa0218040 MtrunA17_Chr3g0129041 65.176 313 106 2 8 318 11 322 5.33e-138 393
Msa0218040 MtrunA17_Chr7g0244461 59.032 310 126 1 9 318 4 312 6.44e-135 385
Msa0218040 MtrunA17_Chr5g0438021 60.952 315 119 4 7 318 12 325 6.41e-134 383
Msa0218040 MtrunA17_Chr7g0244451 57.419 310 131 1 9 318 8 316 1.18e-129 372
Msa0218040 MtrunA17_Chr3g0106921 55.140 321 140 3 1 318 1 320 2.25e-127 366
Msa0218040 MtrunA17_Chr1g0213061 55.625 320 139 2 1 318 3 321 4.14e-123 356
Msa0218040 MtrunA17_Chr3g0106901 58.654 312 126 2 7 318 15 323 1.41e-119 347
Msa0218040 MtrunA17_Chr3g0114451 55.449 312 138 1 7 318 8 318 2.98e-119 345
Msa0218040 MtrunA17_Chr4g0013171 55.096 314 138 3 7 318 9 321 9.62e-119 344
Msa0218040 MtrunA17_Chr5g0432441 56.667 300 125 3 23 318 28 326 6.89e-116 337
Msa0218040 MtrunA17_Chr5g0432351 56.623 302 126 4 21 318 21 321 1.86e-115 336
Msa0218040 MtrunA17_Chr7g0224371 53.651 315 142 4 6 318 10 322 6.43e-115 335
Msa0218040 MtrunA17_Chr5g0432341 56.954 302 125 4 21 318 21 321 7.18e-115 335
Msa0218040 MtrunA17_Chr4g0013201 52.941 306 143 1 13 318 34 338 1.01e-114 335
Msa0218040 MtrunA17_Chr4g0038171 53.481 316 140 3 7 318 9 321 1.25e-113 332
Msa0218040 MtrunA17_Chr4g0043391 53.822 314 138 3 9 318 14 324 1.54e-113 331
Msa0218040 MtrunA17_Chr5g0404721 52.492 301 136 1 25 318 30 330 1.08e-112 329
Msa0218040 MtrunA17_Chr4g0013181 55.290 293 128 3 28 318 62 353 4.84e-112 328
Msa0218040 MtrunA17_Chr5g0432331 55.960 302 128 4 21 318 52 352 2.46e-111 327
Msa0218040 MtrunA17_Chr3g0107881 50.470 319 154 3 4 318 6 324 4.37e-111 325
Msa0218040 MtrunA17_Chr4g0038151 51.724 319 147 4 4 318 6 321 2.67e-110 323
Msa0218040 MtrunA17_Chr4g0013191 53.333 300 136 3 22 318 42 340 1.24e-109 322
Msa0218040 MtrunA17_Chr4g0038161 54.153 301 133 3 22 318 21 320 1.59e-109 321
Msa0218040 MtrunA17_Chr8g0372601 50.831 301 141 1 25 318 30 330 6.38e-108 317
Msa0218040 MtrunA17_Chr5g0402581 51.402 321 146 4 6 318 8 326 6.26e-106 312
Msa0218040 MtrunA17_Chr5g0402571 52.532 316 137 5 11 318 16 326 2.82e-105 310
Msa0218040 MtrunA17_Chr1g0175561 49.837 307 145 3 21 318 20 326 2.22e-103 306
Msa0218040 MtrunA17_Chr1g0155611 51.987 302 137 2 25 318 46 347 4.19e-102 303
Msa0218040 MtrunA17_Chr2g0299691 49.226 323 150 4 8 318 38 358 1.34e-99 297
Msa0218040 MtrunA17_Chr4g0070951 48.598 321 152 4 8 318 12 329 1.57e-99 296
Msa0218040 MtrunA17_Chr7g0258811 47.302 315 157 3 13 318 57 371 2.38e-99 297
Msa0218040 MtrunA17_Chr2g0292151 48.208 307 151 2 20 318 24 330 5.36e-98 293
Msa0218040 MtrunA17_Chr2g0292211 47.634 317 155 3 13 318 25 341 8.58e-98 293
Msa0218040 MtrunA17_Chr2g0292271 47.712 306 152 2 21 318 25 330 5.88e-96 288
Msa0218040 MtrunA17_Chr2g0292111 47.557 307 153 2 20 318 24 330 1.48e-95 286
Msa0218040 MtrunA17_Chr4g0068621 46.535 303 157 1 21 318 34 336 2.79e-95 285
Msa0218040 MtrunA17_Chr2g0292251 46.906 307 155 2 20 318 23 329 4.43e-95 285
Msa0218040 MtrunA17_Chr2g0292181 45.679 324 168 2 3 318 16 339 6.28e-94 283
Msa0218040 MtrunA17_Chr2g0291751 47.799 318 155 4 9 318 16 330 7.79e-94 282
Msa0218040 MtrunA17_Chr2g0292261 45.679 324 168 2 3 318 7 330 7.82e-93 280
Msa0218040 MtrunA17_Chr5g0400331 48.026 304 144 3 28 318 29 331 1.05e-92 278
Msa0218040 MtrunA17_Chr2g0292291 45.846 325 167 3 3 318 8 332 1.14e-92 279
Msa0218040 MtrunA17_Chr2g0292241 45.541 314 163 2 13 318 17 330 1.15e-92 279
Msa0218040 MtrunA17_Chr2g0292091 46.382 304 155 2 23 318 22 325 2.69e-92 278
Msa0218040 MtrunA17_Chr4g0013721 46.732 306 152 4 20 318 95 396 4.52e-90 275
Msa0218040 MtrunA17_Chr5g0432401 46.226 318 125 7 6 317 8 285 2.67e-89 268
Msa0218040 MtrunA17_Chr2g0292101 45.724 304 157 2 23 318 23 326 6.46e-88 267
Msa0218040 MtrunA17_Chr3g0128061 42.415 323 175 4 6 318 5 326 2.98e-85 259
Msa0218040 MtrunA17_Chr5g0421411 45.614 285 144 5 45 318 1 285 2.53e-84 256
Msa0218040 MtrunA17_Chr4g0013821 43.279 305 162 4 21 318 24 324 9.05e-84 256
Msa0218040 MtrunA17_Chr6g0477071 41.611 298 167 6 22 318 51 342 9.17e-82 251
Msa0218040 MtrunA17_Chr4g0024121 41.159 328 181 5 1 318 1 326 3.27e-80 246
Msa0218040 MtrunA17_Chr2g0322861 42.500 320 172 6 8 318 70 386 1.94e-79 246
Msa0218040 MtrunA17_Chr1g0204011 39.506 324 178 8 3 318 9 322 8.07e-76 235
Msa0218040 MtrunA17_Chr4g0063671 40.798 326 173 7 7 318 1 320 7.87e-75 233
Msa0218040 MtrunA17_Chr5g0401431 39.818 329 185 5 1 317 1 328 7.48e-74 230
Msa0218040 MtrunA17_Chr7g0268501 40.439 319 178 4 7 318 7 320 9.89e-74 229
Msa0218040 MtrunA17_Chr7g0268521 40.439 319 178 4 7 318 7 320 9.89e-74 229
Msa0218040 MtrunA17_Chr7g0254201 39.672 305 165 5 23 318 18 312 1.36e-73 229
Msa0218040 MtrunA17_Chr1g0194151 41.212 330 176 7 1 318 10 333 1.38e-73 230
Msa0218040 MtrunA17_Chr6g0460711 40.881 318 178 6 6 318 2 314 2.93e-73 228
Msa0218040 MtrunA17_Chr8g0371381 39.935 308 167 6 20 318 21 319 2.96e-73 228
Msa0218040 MtrunA17_Chr1g0148021 39.752 322 182 5 7 318 9 328 4.80e-73 228
Msa0218040 MtrunA17_Chr2g0279041 41.860 301 158 6 29 318 31 325 1.75e-72 226
Msa0218040 MtrunA17_Chr5g0405991 39.144 327 176 7 1 318 4 316 1.81e-72 226
Msa0218040 MtrunA17_Chr1g0157051 40.000 320 173 8 8 317 7 317 2.90e-72 226
Msa0218040 MtrunA17_Chr1g0188431 38.365 318 185 7 6 318 16 327 1.45e-71 224
Msa0218040 MtrunA17_Chr7g0254231 39.597 298 172 6 23 318 20 311 6.42e-70 219
Msa0218040 MtrunA17_Chr1g0194271 40.059 337 177 7 1 318 10 340 1.85e-69 219
Msa0218040 MtrunA17_Chr7g0225221 37.578 322 195 4 1 318 1 320 2.67e-69 218
Msa0218040 MtrunA17_Chr1g0164891 41.254 303 162 6 29 318 93 392 1.06e-68 219
Msa0218040 MtrunA17_Chr6g0467161 38.889 324 180 8 7 318 19 336 1.19e-68 217
Msa0218040 MtrunA17_Chr4g0053871 39.482 309 181 4 14 317 16 323 6.92e-67 212
Msa0218040 MtrunA17_Chr4g0046131 38.462 325 187 7 4 316 21 344 1.24e-66 212
Msa0218040 MtrunA17_Chr2g0296621 37.304 319 178 7 10 318 35 341 7.01e-66 210
Msa0218040 MtrunA17_Chr4g0076211 38.384 297 166 7 30 318 46 333 1.30e-63 204
Msa0218040 MtrunA17_Chr1g0197411 36.478 318 191 5 11 318 14 330 1.35e-63 204
Msa0218040 MtrunA17_Chr3g0114461 54.891 184 83 0 135 318 9 192 6.61e-63 197
Msa0218040 MtrunA17_Chr4g0072091 38.870 301 170 5 24 314 23 319 6.85e-62 199
Msa0218040 MtrunA17_Chr1g0181511 34.551 301 190 4 23 318 25 323 1.14e-61 199
Msa0218040 MtrunA17_Chr4g0075241 34.437 302 189 4 24 318 28 327 2.34e-61 198
Msa0218040 MtrunA17_Chr2g0278671 38.907 311 170 9 21 318 20 323 4.85e-61 197
Msa0218040 MtrunA17_Chr3g0136751 35.780 327 196 3 1 318 1 322 5.65e-60 194
Msa0218040 MtrunA17_Chr2g0316481 36.527 334 170 9 25 318 76 407 7.18e-60 197
Msa0218040 MtrunA17_Chr6g0467121 38.514 296 174 6 25 318 74 363 8.22e-60 196
Msa0218040 MtrunA17_Chr2g0316791 36.527 334 170 9 25 318 76 407 1.83e-59 196
Msa0218040 MtrunA17_Chr1g0181481 34.884 301 189 4 23 318 25 323 2.48e-59 193
Msa0218040 MtrunA17_Chr2g0316831 36.336 333 170 9 25 317 112 442 9.08e-59 195
Msa0218040 MtrunA17_Chr6g0467251 35.849 318 191 6 7 314 10 324 1.45e-58 191
Msa0218040 MtrunA17_Chr1g0181431 33.555 301 193 4 23 318 25 323 2.52e-58 190
Msa0218040 MtrunA17_Chr6g0451571 34.839 310 185 6 21 318 30 334 4.48e-58 190
Msa0218040 MtrunA17_Chr2g0279061 42.912 261 132 6 69 318 12 266 2.32e-57 186
Msa0218040 MtrunA17_Chr2g0329641 34.441 331 192 7 7 318 11 335 3.12e-57 187
Msa0218040 MtrunA17_Chr8g0364291 34.906 318 196 6 10 318 9 324 3.88e-57 187
Msa0218040 MtrunA17_Chr2g0316191 34.925 335 175 8 25 318 76 408 4.03e-57 189
Msa0218040 MtrunA17_Chr4g0074851 33.028 327 204 6 5 318 4 328 1.99e-52 175
Msa0218040 MtrunA17_Chr2g0311251 34.036 332 177 8 25 317 11 339 4.13e-52 174
Msa0218040 MtrunA17_Chr2g0311241 31.467 375 174 8 23 317 58 429 7.71e-52 176
Msa0218040 MtrunA17_Chr5g0413051 33.968 315 181 7 23 318 71 377 3.11e-51 173
Msa0218040 MtrunA17_Chr2g0292281 40.191 209 107 4 118 318 18 216 5.43e-44 150
Msa0218040 MtrunA17_Chr3g0114491 63.303 109 40 0 24 132 17 125 1.01e-43 146
Msa0218040 MtrunA17_Chr2g0316141 35.793 271 149 10 71 318 11 279 1.53e-40 143
Msa0218040 MtrunA17_Chr2g0316181 35.793 271 149 10 71 318 11 279 1.53e-40 143
Msa0218040 MtrunA17_Chr2g0316811 34.979 243 132 5 25 243 50 290 4.25e-40 143
Msa0218040 MtrunA17_Chr4g0004591 28.308 325 161 5 22 317 74 355 2.83e-38 139
Msa0218040 MtrunA17_Chr5g0432321 52.137 117 55 1 146 262 17 132 1.19e-33 120
Msa0218040 MtrunA17_Chr3g0104101 41.096 146 73 7 185 318 5 149 2.23e-24 96.7
Msa0218040 MtrunA17_Chr1g0181491 42.241 116 61 3 22 135 9 120 6.55e-24 95.5
Msa0218040 MtrunA17_Chr5g0432361 50.000 90 43 2 229 317 20 108 2.35e-22 90.1
Msa0218040 MtrunA17_Chr1g0181501 29.630 189 126 4 135 318 3 189 4.86e-21 89.0
Msa0218040 MtrunA17_Chr7g0254181 41.905 105 57 2 87 191 22 122 1.61e-20 86.7
Msa0218040 MtrunA17_Chr4g0013811 47.297 74 39 0 136 209 6 79 7.80e-18 77.0
Msa0218040 MtrunA17_Chr6g0468731 52.778 72 30 2 64 135 22 89 5.39e-17 75.1
Msa0218040 MtrunA17_Chr1g0196191 55.224 67 29 1 67 133 27 92 1.58e-15 72.8
Msa0218040 MtrunA17_Chr3g0137751 25.000 272 138 8 40 297 91 310 2.23e-15 75.5
Msa0218040 MtrunA17_Chr7g0268491 43.529 85 46 1 234 318 15 97 4.22e-13 64.3
Msa0218040 MtrunA17_Chr7g0254171 29.545 132 92 1 71 202 4 134 1.20e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0218040 AT5G05340.1 62.416 298 109 2 23 318 28 324 2.05e-133 382
Msa0218040 AT5G58400.1 56.688 314 132 3 8 318 13 325 1.39e-119 347
Msa0218040 AT5G58390.1 57.827 313 128 4 9 318 5 316 1.12e-115 337
Msa0218040 AT4G36430.1 49.020 306 149 1 20 318 25 330 1.07e-108 320
Msa0218040 AT5G66390.1 48.485 330 156 2 3 318 4 333 3.85e-108 318
Msa0218040 AT1G14550.1 50.000 318 156 3 3 318 5 321 6.68e-108 317
Msa0218040 AT3G50990.1 50.980 306 143 1 20 318 36 341 1.23e-106 315
Msa0218040 AT2G18140.1 46.269 335 163 3 1 318 1 335 3.72e-105 311
Msa0218040 AT1G44970.1 53.642 302 132 2 25 318 45 346 4.78e-105 311
Msa0218040 AT2G18150.1 49.169 301 146 1 25 318 36 336 9.82e-105 310
Msa0218040 AT1G14540.1 48.243 313 161 1 7 318 3 315 2.72e-104 308
Msa0218040 AT5G06720.1 47.256 328 163 3 1 318 6 333 2.49e-103 306
Msa0218040 AT1G49570.1 51.325 302 136 4 23 314 46 346 4.61e-101 301
Msa0218040 AT5G06730.1 47.134 314 157 3 13 318 22 334 9.85e-100 298
Msa0218040 AT4G08780.1 49.191 309 145 5 21 318 20 327 5.19e-99 295
Msa0218040 AT2G38390.1 47.452 314 156 3 14 318 20 333 2.22e-98 294
Msa0218040 AT3G32980.1 47.352 321 159 3 8 318 14 334 1.01e-97 292
Msa0218040 AT4G08770.1 47.403 308 152 3 21 318 20 327 6.94e-96 288
Msa0218040 AT3G49120.1 46.584 322 162 3 7 318 14 335 1.32e-94 285
Msa0218040 AT5G19890.1 46.749 323 156 5 6 318 11 327 2.17e-93 280
Msa0218040 AT3G49110.1 45.342 322 166 3 7 318 15 336 1.14e-92 280
Msa0218040 AT2G38380.1 45.768 319 164 3 9 318 15 333 1.86e-92 279
Msa0218040 AT4G16270.1 45.981 311 159 3 15 318 40 348 4.70e-91 275
Msa0218040 AT2G35380.1 45.593 329 159 5 9 318 8 335 7.69e-90 272
Msa0218040 AT2G22420.1 43.077 325 172 5 1 318 1 319 2.50e-89 270
Msa0218040 AT1G68850.1 42.020 307 164 6 25 318 30 335 1.68e-87 266
Msa0218040 AT5G19880.1 44.582 323 165 6 8 318 9 329 8.57e-84 256
Msa0218040 AT3G03670.1 42.271 317 173 4 9 318 8 321 8.79e-79 243
Msa0218040 AT5G15180.1 39.812 319 182 4 7 318 14 329 7.22e-78 241
Msa0218040 AT5G17820.1 41.509 318 168 6 8 318 7 313 3.15e-77 239
Msa0218040 AT1G05260.1 40.461 304 171 4 23 318 24 325 7.22e-77 238
Msa0218040 AT5G64120.1 44.932 296 151 5 29 318 39 328 2.72e-76 237
Msa0218040 AT5G17820.2 41.509 318 168 6 8 318 60 366 2.78e-76 238
Msa0218040 AT5G42180.1 39.752 322 180 6 4 317 1 316 3.98e-76 236
Msa0218040 AT3G01190.1 41.463 328 175 8 1 318 1 321 7.01e-76 236
Msa0218040 AT4G11290.1 39.688 320 184 3 7 318 8 326 9.49e-76 235
Msa0218040 AT5G14130.1 40.248 323 173 7 8 318 16 330 4.76e-75 234
Msa0218040 AT5G14130.2 40.248 323 173 7 8 318 57 371 1.25e-74 234
Msa0218040 AT3G21770.1 38.284 303 177 4 20 314 24 324 1.66e-72 227
Msa0218040 AT1G71695.1 41.837 294 165 5 25 314 44 335 8.11e-72 226
Msa0218040 AT1G05240.1 39.414 307 166 6 25 318 26 325 1.04e-71 225
Msa0218040 AT1G05250.1 39.414 307 166 6 25 318 26 325 1.04e-71 225
Msa0218040 AT2G18980.1 40.379 317 172 7 13 318 13 323 5.34e-70 221
Msa0218040 AT2G41480.1 38.889 324 180 7 7 318 11 328 1.16e-69 220
Msa0218040 AT4G26010.1 39.375 320 181 8 1 318 1 309 1.70e-69 219
Msa0218040 AT4G26010.2 39.062 320 182 8 1 318 29 337 3.29e-69 219
Msa0218040 AT3G28200.1 39.404 302 179 4 20 318 16 316 4.67e-69 218
Msa0218040 AT4G30170.1 40.065 307 169 6 22 318 24 325 1.14e-68 217
Msa0218040 AT5G67400.1 37.771 323 189 5 7 318 8 329 1.55e-68 217
Msa0218040 AT4G37530.1 39.297 313 169 6 21 318 23 329 1.57e-68 217
Msa0218040 AT5G40150.1 38.614 303 181 4 20 318 27 328 1.57e-68 217
Msa0218040 AT5G51890.1 38.006 321 180 8 7 318 12 322 1.53e-67 214
Msa0218040 AT2G35380.2 47.755 245 114 4 87 318 4 247 3.28e-67 211
Msa0218040 AT3G49960.1 38.608 316 183 4 13 318 15 329 7.87e-67 213
Msa0218040 AT5G39580.1 41.751 297 160 6 29 318 29 319 1.93e-66 211
Msa0218040 AT4G33420.1 39.322 295 172 6 25 318 37 325 2.94e-66 211
Msa0218040 AT4G25980.1 38.037 326 183 8 6 318 7 326 1.00e-65 209
Msa0218040 AT4G37520.1 37.621 311 173 6 23 318 25 329 1.01e-65 210
Msa0218040 AT4G33420.2 39.322 295 172 6 25 318 84 372 1.08e-65 211
Msa0218040 AT5G64110.1 40.361 332 173 7 4 318 7 330 6.09e-65 208
Msa0218040 AT1G30870.1 37.667 300 168 7 25 314 51 341 2.30e-64 207
Msa0218040 AT1G77100.1 39.812 319 180 9 7 318 6 319 7.90e-64 204
Msa0218040 AT4G37520.2 37.299 311 171 7 23 318 25 326 1.92e-63 204
Msa0218040 AT5G64100.1 38.806 335 175 8 5 318 6 331 3.36e-63 203
Msa0218040 AT2G39040.1 39.228 311 172 7 22 318 43 350 4.21e-63 204
Msa0218040 AT4G17690.1 36.242 298 185 4 25 318 26 322 1.94e-62 201
Msa0218040 AT4G33870.1 33.036 336 177 7 23 314 66 397 2.55e-62 203
Msa0218040 AT2G34060.1 37.621 311 181 6 18 316 35 344 4.23e-62 201
Msa0218040 AT1G34510.1 38.111 307 182 6 13 318 10 309 4.78e-62 200
Msa0218040 AT2G24800.1 35.948 306 181 4 23 318 29 329 8.96e-60 194
Msa0218040 AT4G31760.1 34.969 326 194 4 2 318 10 326 1.18e-59 194
Msa0218040 AT4G37530.2 41.016 256 141 3 21 266 23 278 2.34e-59 192
Msa0218040 AT5G47000.1 34.796 319 199 5 8 318 13 330 1.04e-58 192
Msa0218040 AT5G22410.1 36.086 327 188 10 1 315 5 322 1.34e-58 191
Msa0218040 AT1G24110.1 35.016 317 195 5 11 318 8 322 7.04e-57 187
Msa0218040 AT5G24070.1 36.242 298 171 6 33 318 45 335 6.52e-56 185
Msa0218040 AT2G37130.1 34.098 305 189 6 22 318 27 327 5.03e-55 182
Msa0218040 AT3G17070.1 35.783 313 177 9 22 318 35 339 5.81e-55 182
Msa0218040 AT4G21960.1 32.822 326 204 5 6 318 4 327 2.03e-54 181
Msa0218040 AT2G37130.2 34.114 299 185 6 28 318 2 296 3.95e-54 179
Msa0218040 AT2G43480.1 35.452 299 172 7 33 318 45 335 1.42e-53 179
Msa0218040 AT4G25980.2 43.316 187 102 3 6 192 52 234 2.87e-45 155
Msa0218040 AT3G17070.2 35.821 268 147 10 67 318 18 276 1.52e-41 145
Msa0218040 AT5G39580.2 41.117 197 107 4 29 225 29 216 1.65e-41 144
Msa0218040 AT2G24800.2 39.785 186 106 2 23 206 29 210 2.21e-40 142
Msa0218040 AT3G42570.1 37.634 93 57 1 101 192 57 149 9.21e-15 70.9
Msa0218040 AT4G35970.1 26.482 253 130 9 59 300 33 240 3.86e-13 68.9
Msa0218040 AT4G35970.2 27.727 220 104 8 59 267 33 208 4.23e-11 62.4

Find 86 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTAACAAATAATTTGTTTA+AGG 0.133122 2_1:-65064416 None:intergenic
AATGACCTTACTGTCCTTTC+TGG 0.161088 2_1:+65066223 Msa0218040:CDS
TTCTGGTGGTGACATAAATT+TGG 0.222257 2_1:+65066354 Msa0218040:CDS
TTTGTGTTTCTTTAATTTGA+TGG 0.252614 2_1:+65066740 Msa0218040:three_prime_UTR
GATGACACTGCTACCTTTAC+CGG 0.263088 2_1:+65064727 Msa0218040:CDS
CAACTTAATCCTAACTTCTA+TGG 0.286741 2_1:+65064478 Msa0218040:CDS
TGTTGGTTCTCAAGTTTCTT+TGG 0.288110 2_1:+65066480 Msa0218040:CDS
CAGAAGATGGTCCAGGGATT+TGG 0.307170 2_1:-65066153 None:intergenic
AGCAGACAAATTTGAAACTT+TGG 0.312130 2_1:+65064193 Msa0218040:five_prime_UTR
GGTCATTTCCCTGCGCACTA+TGG 0.317039 2_1:-65064524 None:intergenic
CAAGTTCTTCCATAGAAGTT+AGG 0.319481 2_1:-65064487 None:intergenic
AGTCCTCTAACTGGGACTAA+TGG 0.328102 2_1:+65066577 Msa0218040:CDS
AATGACCTTGTTGCTAATAA+AGG 0.329690 2_1:+65066424 Msa0218040:CDS
AAATCAAATTGTCCTACTTC+TGG 0.333770 2_1:+65066337 Msa0218040:CDS
TTGTGATGGATCAATCTTAT+TGG 0.342695 2_1:+65064705 Msa0218040:CDS
GCAGACAAATTTGAAACTTT+GGG 0.346926 2_1:+65064194 Msa0218040:five_prime_UTR
GTCCTCTAACTGGGACTAAT+GGG 0.358033 2_1:+65066578 Msa0218040:CDS
TGCATGCACCGTCACAATAA+TGG 0.360164 2_1:+65064280 Msa0218040:five_prime_UTR
AGTAGAATTAGTCCTCTAAC+TGG 0.369986 2_1:+65066568 Msa0218040:CDS
GTTCTCAAGTTTCTTTGGTT+AGG 0.371031 2_1:+65066485 Msa0218040:CDS
TCCCCATTAGTCCCAGTTAG+AGG 0.372964 2_1:-65066580 None:intergenic
AGCTTCATTATTGATAGCTT+TGG 0.373071 2_1:-65064545 None:intergenic
TTTCCCTGCGCACTATGGTT+TGG 0.378494 2_1:-65064519 None:intergenic
CGCACTATGGTTTGGAGGCT+AGG 0.382068 2_1:-65064511 None:intergenic
ATCAATAATGAAGCTCGAAT+TGG 0.383740 2_1:+65064553 Msa0218040:CDS
ACTAAGCAATATATAGAGAT+AGG 0.390916 2_1:-65064315 None:intergenic
GTTTGTCTTACTTAAATCAA+TGG 0.396273 2_1:+65066797 Msa0218040:three_prime_UTR
ATTTCCATAACTTCTCTCAT+TGG 0.397402 2_1:+65064370 Msa0218040:five_prime_UTR
AATACCAATGAGAGAAGTTA+TGG 0.398012 2_1:-65064374 None:intergenic
AATCTCTATAAAATCTTGAA+TGG 0.404314 2_1:-65066650 None:intergenic
ATAGAGATAGGAATTTGTTT+AGG 0.407055 2_1:-65064303 None:intergenic
TAATTGTTGTCAAAAGTAAC+TGG 0.409448 2_1:-65066397 None:intergenic
TTAGAGAATCAAAGTCATAA+AGG 0.409731 2_1:+65066679 Msa0218040:three_prime_UTR
AAGATAGTGTTGTAACAAAA+GGG 0.415119 2_1:-65064024 None:intergenic
CCCTGCGCACTATGGTTTGG+AGG 0.416029 2_1:-65064516 None:intergenic
CATAAAGGTTGACTAGATCT+TGG 0.416665 2_1:+65066694 Msa0218040:three_prime_UTR
AAGTATGAAGTAGTCGTTGC+TGG 0.428466 2_1:-65063990 None:intergenic
TTGTGTAAAAGTTATTATTA+CGG 0.433761 2_1:+65066876 Msa0218040:three_prime_UTR
TATCGGGTAAGTCAAGTAAT+GGG 0.436429 2_1:+65064080 Msa0218040:five_prime_UTR
GAAGATAGTGTTGTAACAAA+AGG 0.438100 2_1:-65064025 None:intergenic
GGGAGATTAGAAAGAATTGC+AGG 0.441402 2_1:+65066599 Msa0218040:CDS
TTACATTTACAAAGCAATCA+TGG 0.442899 2_1:-65064600 None:intergenic
AAGAGACTTTGCAGCTGCTA+TGG 0.475794 2_1:+65066537 Msa0218040:CDS
TACTTTATGGGAGGAACTTG+TGG 0.476515 2_1:-65064138 None:intergenic
ACAAACAACATATATATTTG+TGG 0.485474 2_1:-65066780 None:intergenic
GTTAGGATTAAGTTGTGCAT+TGG 0.489786 2_1:-65064470 None:intergenic
GATAGGTCAGAAGATGGTCC+AGG 0.491263 2_1:-65066160 None:intergenic
TTGACATTCTGCTTGACCTA+TGG 0.493071 2_1:-65066254 None:intergenic
GCCTCCAAACCATAGTGCGC+AGG 0.498639 2_1:+65064515 Msa0218040:CDS
GTCGTTGCTGGGTAGAAGAA+TGG 0.502683 2_1:-65063978 None:intergenic
TTATCGGGTAAGTCAAGTAA+TGG 0.514390 2_1:+65064079 Msa0218040:five_prime_UTR
TAATAGTCAGAAAGAGAAGA+TGG 0.518431 2_1:-65064242 None:intergenic
CTAGCACTTGCAACTAGAGA+TGG 0.521717 2_1:+65064862 Msa0218040:CDS
AACTAGAGATGGAATTGCTC+TGG 0.526745 2_1:+65064873 Msa0218040:CDS
GCAGCAAACAGCCAAATCCC+TGG 0.526990 2_1:+65066142 Msa0218040:CDS
CTTGCATCTCTTCTTCCAAG+TGG 0.553652 2_1:-65066103 None:intergenic
TGCAAAGTTTGTGTCAATGT+TGG 0.554512 2_1:-65066305 None:intergenic
CTTTCTGGTGCACACACCAT+AGG 0.556404 2_1:+65066238 Msa0218040:CDS
GCACTATGGTTTGGAGGCTA+GGG 0.562885 2_1:-65064510 None:intergenic
TCAAATTGTCCTACTTCTGG+TGG 0.566583 2_1:+65066340 Msa0218040:CDS
TGTTTAGGCCATTATTGTGA+CGG 0.566598 2_1:-65064288 None:intergenic
CCAAGTGGTACTACCCATGT+TGG 0.572648 2_1:-65066088 None:intergenic
CCAACATGGGTAGTACCACT+TGG 0.573801 2_1:+65066088 Msa0218040:CDS
TGTAGCTTGGAGGACCAACA+TGG 0.577701 2_1:+65066074 Msa0218040:intron
TAGACCTTTGTTTCTAAACA+TGG 0.579085 2_1:-65066194 None:intergenic
TTTATGTCACCACCAGAAGT+AGG 0.583827 2_1:-65066349 None:intergenic
ACATGGTAGTGAGTGTGGAT+AGG 0.587508 2_1:-65066177 None:intergenic
CCTCCAAACCATAGTGCGCA+GGG 0.588313 2_1:+65064516 Msa0218040:CDS
TTTATGGGAGGAACTTGTGG+AGG 0.590542 2_1:-65064135 None:intergenic
AGATAGTGTTGTAACAAAAG+GGG 0.592502 2_1:-65064023 None:intergenic
AGTATGAAGTAGTCGTTGCT+GGG 0.595877 2_1:-65063989 None:intergenic
GATAGTGTTGTAACAAAAGG+GGG 0.599441 2_1:-65064022 None:intergenic
ACTACCATGTTTAGAAACAA+AGG 0.599660 2_1:+65066190 Msa0218040:CDS
TGACTAGATCTTGGACACAT+TGG 0.605648 2_1:+65066703 Msa0218040:three_prime_UTR
GTGCACCAGAAAGGACAGTA+AGG 0.606249 2_1:-65066228 None:intergenic
AGTGTGGATAGGTCAGAAGA+TGG 0.621043 2_1:-65066166 None:intergenic
TCTAAACATGGTAGTGAGTG+TGG 0.630401 2_1:-65066182 None:intergenic
GTAGAATTAGTCCTCTAACT+GGG 0.630567 2_1:+65066569 Msa0218040:CDS
AGAGAAGTTATGGAAATGTG+AGG 0.643958 2_1:-65064364 None:intergenic
GAAGTCCTTTATTAGCAACA+AGG 0.654884 2_1:-65066429 None:intergenic
TCCTCTAACTGGGACTAATG+GGG 0.661630 2_1:+65066579 Msa0218040:CDS
CTATGGTGTGTGCACCAGAA+AGG 0.672627 2_1:-65066237 None:intergenic
ATAGGTCAGAAGATGGTCCA+GGG 0.677432 2_1:-65066159 None:intergenic
AATATCAGCACAAGAGACAG+TGG 0.690128 2_1:-65064839 None:intergenic
ACTGGAGAGACAGAATCAAG+AGG 0.693800 2_1:-65066379 None:intergenic
GTAGCTTGGAGGACCAACAT+GGG 0.713947 2_1:+65066075 Msa0218040:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTCTTTGTTTTATTTTAT+CGG + chr2_1:65064063-65064082 Msa0218040:five_prime_UTR 10.0%
!! CTACATTAAATAAAATAAAT+CGG - chr2_1:65065580-65065599 None:intergenic 10.0%
!! ATATAATTAGAGAAATTTAT+TGG - chr2_1:65065843-65065862 None:intergenic 10.0%
!!! TTTCTTTGTTTTATTTTATC+GGG + chr2_1:65064064-65064083 Msa0218040:five_prime_UTR 15.0%
!!! CTTTTTTATGTTTACTTTAT+GGG - chr2_1:65064153-65064172 None:intergenic 15.0%
!!! TCTTTTTTATGTTTACTTTA+TGG - chr2_1:65064154-65064173 None:intergenic 15.0%
!!! TGGTATTTTTATATTGAATA+TGG + chr2_1:65064390-65064409 Msa0218040:exon 15.0%
!!! TGTAACAAATAATTTGTTTA+AGG - chr2_1:65064419-65064438 None:intergenic 15.0%
!!! AATTTATTTGCTTATTTTGT+AGG + chr2_1:65064681-65064700 Msa0218040:intron 15.0%
!!! ATTTATTTGCTTATTTTGTA+GGG + chr2_1:65064682-65064701 Msa0218040:intron 15.0%
!! AAAATACATCACATAATATA+TGG + chr2_1:65065364-65065383 Msa0218040:intron 15.0%
!!! CGATTTATTTTATTTAATGT+AGG + chr2_1:65065578-65065597 Msa0218040:intron 15.0%
!! TATATTGTGTTTAATTCATT+CGG + chr2_1:65065717-65065736 Msa0218040:intron 15.0%
!!! AATTAATTAATTTTGTAGCT+TGG + chr2_1:65066061-65066080 Msa0218040:intron 15.0%
!! TTGTGTAAAAGTTATTATTA+CGG + chr2_1:65066876-65066895 Msa0218040:three_prime_UTR 15.0%
!!! TTTTCAACATTATCTTAAAG+TGG - chr2_1:65064628-65064647 None:intergenic 20.0%
!! AAGAAAAAAGAAGAAAGAAA+TGG - chr2_1:65064907-65064926 None:intergenic 20.0%
!! TTAAACTATCTACAACATAA+GGG - chr2_1:65064930-65064949 None:intergenic 20.0%
!! ATTAAACTATCTACAACATA+AGG - chr2_1:65064931-65064950 None:intergenic 20.0%
!! TGCTGAAAAATAAAAAATTG+TGG + chr2_1:65064971-65064990 Msa0218040:intron 20.0%
!! GCTGAAAAATAAAAAATTGT+GGG + chr2_1:65064972-65064991 Msa0218040:intron 20.0%
!!! AATCAATAACATCTCTAAAA+AGG - chr2_1:65065058-65065077 None:intergenic 20.0%
!!! TTCTTTATTTCTTTCTTACT+TGG + chr2_1:65065122-65065141 Msa0218040:intron 20.0%
!!! GTTTCCTATAATTTTGTTTT+TGG + chr2_1:65065214-65065233 Msa0218040:intron 20.0%
!! GATAAAAAATATGTGAGAAA+AGG - chr2_1:65065283-65065302 None:intergenic 20.0%
!!! AAATATCTATTTTATCTGGA+GGG - chr2_1:65065338-65065357 None:intergenic 20.0%
!!! AAAATATCTATTTTATCTGG+AGG - chr2_1:65065339-65065358 None:intergenic 20.0%
!!! TGCAAAATATCTATTTTATC+TGG - chr2_1:65065342-65065361 None:intergenic 20.0%
!! ACTTGATACATGAATATAAA+TGG + chr2_1:65065652-65065671 Msa0218040:intron 20.0%
!! ACAATATACTAACTAGTTAT+GGG - chr2_1:65065705-65065724 None:intergenic 20.0%
!! TTAATTCATTCGGTTAAAAT+CGG + chr2_1:65065727-65065746 Msa0218040:intron 20.0%
!! ATAGGACTATAGTTTATATT+TGG - chr2_1:65065984-65066003 None:intergenic 20.0%
!! AAATATAAACTATAGTCCTA+TGG + chr2_1:65065983-65066002 Msa0218040:intron 20.0%
!! AATCTCTATAAAATCTTGAA+TGG - chr2_1:65066653-65066672 None:intergenic 20.0%
!!! TTTGTGTTTCTTTAATTTGA+TGG + chr2_1:65066740-65066759 Msa0218040:three_prime_UTR 20.0%
!! ACAAACAACATATATATTTG+TGG - chr2_1:65066783-65066802 None:intergenic 20.0%
! AAGATAGTGTTGTAACAAAA+GGG - chr2_1:65064027-65064046 None:intergenic 25.0%
! AAGAAAAAAGCTGCAAATAA+TGG - chr2_1:65064050-65064069 None:intergenic 25.0%
! GTGAGATAAAAAGAATACAA+TGG - chr2_1:65064109-65064128 None:intergenic 25.0%
!! TTTTATGTTTACTTTATGGG+AGG - chr2_1:65064150-65064169 None:intergenic 25.0%
!! ATAGAGATAGGAATTTGTTT+AGG - chr2_1:65064306-65064325 None:intergenic 25.0%
! ACTAAGCAATATATAGAGAT+AGG - chr2_1:65064318-65064337 None:intergenic 25.0%
! TTACATTTACAAAGCAATCA+TGG - chr2_1:65064603-65064622 None:intergenic 25.0%
! CATGCCAAAAACAAAATTAT+AGG - chr2_1:65065221-65065240 None:intergenic 25.0%
!!! CTATAATTTTGTTTTTGGCA+TGG + chr2_1:65065219-65065238 Msa0218040:intron 25.0%
!!! TGTCAACTCTTGTTATTTTA+TGG + chr2_1:65065310-65065329 Msa0218040:intron 25.0%
!!! GTCAACTCTTGTTATTTTAT+GGG + chr2_1:65065311-65065330 Msa0218040:intron 25.0%
!!! TCAACTCTTGTTATTTTATG+GGG + chr2_1:65065312-65065331 Msa0218040:intron 25.0%
!!! CGGTTTTATTATAAGATGAA+AGG + chr2_1:65065503-65065522 Msa0218040:intron 25.0%
! GCTCAAAAAAAAAAGATGAA+AGG + chr2_1:65065532-65065551 Msa0218040:intron 25.0%
! TGTATTGAGAAAAAAAAGTC+AGG - chr2_1:65065605-65065624 None:intergenic 25.0%
! CAATATACTAACTAGTTATG+GGG - chr2_1:65065704-65065723 None:intergenic 25.0%
! CACAATATACTAACTAGTTA+TGG - chr2_1:65065706-65065725 None:intergenic 25.0%
!!! GTATTTTGTACTTGCTTTTT+TGG - chr2_1:65065956-65065975 None:intergenic 25.0%
!! TAATTAATTTTGTAGCTTGG+AGG + chr2_1:65066064-65066083 Msa0218040:intron 25.0%
!!! AGGACAATTTGATTTTCTTA+AGG - chr2_1:65066332-65066351 None:intergenic 25.0%
! TAATTGTTGTCAAAAGTAAC+TGG - chr2_1:65066400-65066419 None:intergenic 25.0%
! TTAGAGAATCAAAGTCATAA+AGG + chr2_1:65066679-65066698 Msa0218040:three_prime_UTR 25.0%
! GTTTGTCTTACTTAAATCAA+TGG + chr2_1:65066797-65066816 Msa0218040:three_prime_UTR 25.0%
AGATAGTGTTGTAACAAAAG+GGG - chr2_1:65064026-65064045 None:intergenic 30.0%
GAAGATAGTGTTGTAACAAA+AGG - chr2_1:65064028-65064047 None:intergenic 30.0%
! AGCAGACAAATTTGAAACTT+TGG + chr2_1:65064193-65064212 Msa0218040:five_prime_UTR 30.0%
! GCAGACAAATTTGAAACTTT+GGG + chr2_1:65064194-65064213 Msa0218040:five_prime_UTR 30.0%
TAATAGTCAGAAAGAGAAGA+TGG - chr2_1:65064245-65064264 None:intergenic 30.0%
ATTTCCATAACTTCTCTCAT+TGG + chr2_1:65064370-65064389 Msa0218040:five_prime_UTR 30.0%
AATACCAATGAGAGAAGTTA+TGG - chr2_1:65064377-65064396 None:intergenic 30.0%
CAACTTAATCCTAACTTCTA+TGG + chr2_1:65064478-65064497 Msa0218040:CDS 30.0%
! AGCTTCATTATTGATAGCTT+TGG - chr2_1:65064548-65064567 None:intergenic 30.0%
ATCAATAATGAAGCTCGAAT+TGG + chr2_1:65064553-65064572 Msa0218040:CDS 30.0%
!! TTGTGATGGATCAATCTTAT+TGG + chr2_1:65064705-65064724 Msa0218040:CDS 30.0%
!!! AGCTTCAACACTAGTTTTAA+TGG - chr2_1:65064809-65064828 None:intergenic 30.0%
CAGCACTTAATGTGTAAATT+TGG - chr2_1:65064956-65064975 None:intergenic 30.0%
! AAAAGGTTAAAAGAGAGTGT+GGG - chr2_1:65065266-65065285 None:intergenic 30.0%
TGATCTTTGATAAAGAGACA+TGG - chr2_1:65065442-65065461 None:intergenic 30.0%
!! AAAATCGGTTTCTAAAACGA+TGG + chr2_1:65065742-65065761 Msa0218040:intron 30.0%
!!! TTGTACTTGCTTTTTTGGAA+TGG - chr2_1:65065951-65065970 None:intergenic 30.0%
AAACTAGTATAATGCACCAT+AGG - chr2_1:65066002-65066021 None:intergenic 30.0%
ACTACCATGTTTAGAAACAA+AGG + chr2_1:65066190-65066209 Msa0218040:CDS 30.0%
!! TAGACCTTTGTTTCTAAACA+TGG - chr2_1:65066197-65066216 None:intergenic 30.0%
AAATCAAATTGTCCTACTTC+TGG + chr2_1:65066337-65066356 Msa0218040:CDS 30.0%
AATGACCTTGTTGCTAATAA+AGG + chr2_1:65066424-65066443 Msa0218040:CDS 30.0%
GATAGTGTTGTAACAAAAGG+GGG - chr2_1:65064025-65064044 None:intergenic 35.0%
TTATCGGGTAAGTCAAGTAA+TGG + chr2_1:65064079-65064098 Msa0218040:five_prime_UTR 35.0%
TATCGGGTAAGTCAAGTAAT+GGG + chr2_1:65064080-65064099 Msa0218040:five_prime_UTR 35.0%
TGTTTAGGCCATTATTGTGA+CGG - chr2_1:65064291-65064310 None:intergenic 35.0%
AGAGAAGTTATGGAAATGTG+AGG - chr2_1:65064367-65064386 None:intergenic 35.0%
! GTTAGGATTAAGTTGTGCAT+TGG - chr2_1:65064473-65064492 None:intergenic 35.0%
CAAGTTCTTCCATAGAAGTT+AGG - chr2_1:65064490-65064509 None:intergenic 35.0%
!!! CTTATTTTGTAGGGTTGTGA+TGG + chr2_1:65064691-65064710 Msa0218040:intron 35.0%
ACCCAACAAAAATTCAGCTA+GGG + chr2_1:65064765-65064784 Msa0218040:CDS 35.0%
!!! CAAGTCATTTTCAAGTAGCT+AGG + chr2_1:65065175-65065194 Msa0218040:intron 35.0%
! AAAGGTTAAAAGAGAGTGTG+GGG - chr2_1:65065265-65065284 None:intergenic 35.0%
GAAAAGGTTAAAAGAGAGTG+TGG - chr2_1:65065267-65065286 None:intergenic 35.0%
TGCAAAGTTTGTGTCAATGT+TGG - chr2_1:65066308-65066327 None:intergenic 35.0%
! TTCTGGTGGTGACATAAATT+TGG + chr2_1:65066354-65066373 Msa0218040:CDS 35.0%
GAAGTCCTTTATTAGCAACA+AGG - chr2_1:65066432-65066451 None:intergenic 35.0%
! TCTGATCAAGCTCTTTTCAA+TGG + chr2_1:65066457-65066476 Msa0218040:CDS 35.0%
!! TGTTGGTTCTCAAGTTTCTT+TGG + chr2_1:65066480-65066499 Msa0218040:CDS 35.0%
GTTCTCAAGTTTCTTTGGTT+AGG + chr2_1:65066485-65066504 Msa0218040:CDS 35.0%
AGTAGAATTAGTCCTCTAAC+TGG + chr2_1:65066568-65066587 Msa0218040:CDS 35.0%
GTAGAATTAGTCCTCTAACT+GGG + chr2_1:65066569-65066588 Msa0218040:CDS 35.0%
CATAAAGGTTGACTAGATCT+TGG + chr2_1:65066694-65066713 Msa0218040:three_prime_UTR 35.0%
AGTATGAAGTAGTCGTTGCT+GGG - chr2_1:65063992-65064011 None:intergenic 40.0%
AAGTATGAAGTAGTCGTTGC+TGG - chr2_1:65063993-65064012 None:intergenic 40.0%
TACTTTATGGGAGGAACTTG+TGG - chr2_1:65064141-65064160 None:intergenic 40.0%
! AGCATTTTTCTCACCGGTAA+AGG - chr2_1:65064743-65064762 None:intergenic 40.0%
TTTACCGGTGAGAAAAATGC+TGG + chr2_1:65064742-65064761 Msa0218040:CDS 40.0%
GACCCAACAAAAATTCAGCT+AGG + chr2_1:65064764-65064783 Msa0218040:CDS 40.0%
! CCCCTAGCTGAATTTTTGTT+GGG - chr2_1:65064769-65064788 None:intergenic 40.0%
CCCAACAAAAATTCAGCTAG+GGG + chr2_1:65064766-65064785 Msa0218040:CDS 40.0%
! ACCCCTAGCTGAATTTTTGT+TGG - chr2_1:65064770-65064789 None:intergenic 40.0%
AATATCAGCACAAGAGACAG+TGG - chr2_1:65064842-65064861 None:intergenic 40.0%
AACTAGAGATGGAATTGCTC+TGG + chr2_1:65064873-65064892 Msa0218040:CDS 40.0%
! AAGGTTAAAAGAGAGTGTGG+GGG - chr2_1:65065264-65065283 None:intergenic 40.0%
AGACATGGCTGACAAAAGAA+TGG - chr2_1:65065427-65065446 None:intergenic 40.0%
TGGTAAGTGACACGATTCAT+AGG + chr2_1:65065672-65065691 Msa0218040:intron 40.0%
! TCTAAACATGGTAGTGAGTG+TGG - chr2_1:65066185-65066204 None:intergenic 40.0%
AATGACCTTACTGTCCTTTC+TGG + chr2_1:65066223-65066242 Msa0218040:CDS 40.0%
TTGACATTCTGCTTGACCTA+TGG - chr2_1:65066257-65066276 None:intergenic 40.0%
TCAAATTGTCCTACTTCTGG+TGG + chr2_1:65066340-65066359 Msa0218040:CDS 40.0%
TTTATGTCACCACCAGAAGT+AGG - chr2_1:65066352-65066371 None:intergenic 40.0%
! CAAGCTCTTTTCAATGGTGT+TGG + chr2_1:65066463-65066482 Msa0218040:CDS 40.0%
GGGAGATTAGAAAGAATTGC+AGG + chr2_1:65066599-65066618 Msa0218040:CDS 40.0%
TGACTAGATCTTGGACACAT+TGG + chr2_1:65066703-65066722 Msa0218040:three_prime_UTR 40.0%
TTTATGGGAGGAACTTGTGG+AGG - chr2_1:65064138-65064157 None:intergenic 45.0%
TGCATGCACCGTCACAATAA+TGG + chr2_1:65064280-65064299 Msa0218040:five_prime_UTR 45.0%
GATGACACTGCTACCTTTAC+CGG + chr2_1:65064727-65064746 Msa0218040:CDS 45.0%
CTAGCACTTGCAACTAGAGA+TGG + chr2_1:65064862-65064881 Msa0218040:CDS 45.0%
GGGGACATGGTTTGAAAACA+AGG - chr2_1:65065245-65065264 None:intergenic 45.0%
CTTGCATCTCTTCTTCCAAG+TGG - chr2_1:65066106-65066125 None:intergenic 45.0%
ATAGGTCAGAAGATGGTCCA+GGG - chr2_1:65066162-65066181 None:intergenic 45.0%
AGTGTGGATAGGTCAGAAGA+TGG - chr2_1:65066169-65066188 None:intergenic 45.0%
! ACATGGTAGTGAGTGTGGAT+AGG - chr2_1:65066180-65066199 None:intergenic 45.0%
ACTGGAGAGACAGAATCAAG+AGG - chr2_1:65066382-65066401 None:intergenic 45.0%
AAGAGACTTTGCAGCTGCTA+TGG + chr2_1:65066537-65066556 Msa0218040:CDS 45.0%
AGTCCTCTAACTGGGACTAA+TGG + chr2_1:65066577-65066596 Msa0218040:CDS 45.0%
GTCCTCTAACTGGGACTAAT+GGG + chr2_1:65066578-65066597 Msa0218040:CDS 45.0%
TCCTCTAACTGGGACTAATG+GGG + chr2_1:65066579-65066598 Msa0218040:CDS 45.0%
!! TATAAATGAAATTATAAAAA+CGG + chr2_1:65065483-65065502 Msa0218040:intron 5.0%
GTCGTTGCTGGGTAGAAGAA+TGG - chr2_1:65063981-65064000 None:intergenic 50.0%
! GCACTATGGTTTGGAGGCTA+GGG - chr2_1:65064513-65064532 None:intergenic 50.0%
TTTCCCTGCGCACTATGGTT+TGG - chr2_1:65064522-65064541 None:intergenic 50.0%
! GGGTCCAGCATTTTTCTCAC+CGG - chr2_1:65064749-65064768 None:intergenic 50.0%
! AAAAGAGAGTGTGGGGGACA+TGG - chr2_1:65065258-65065277 None:intergenic 50.0%
TGTAGCTTGGAGGACCAACA+TGG + chr2_1:65066074-65066093 Msa0218040:intron 50.0%
GTAGCTTGGAGGACCAACAT+GGG + chr2_1:65066075-65066094 Msa0218040:intron 50.0%
CCAAGTGGTACTACCCATGT+TGG - chr2_1:65066091-65066110 None:intergenic 50.0%
CCAACATGGGTAGTACCACT+TGG + chr2_1:65066088-65066107 Msa0218040:CDS 50.0%
CAGAAGATGGTCCAGGGATT+TGG - chr2_1:65066156-65066175 None:intergenic 50.0%
GATAGGTCAGAAGATGGTCC+AGG - chr2_1:65066163-65066182 None:intergenic 50.0%
GTGCACCAGAAAGGACAGTA+AGG - chr2_1:65066231-65066250 None:intergenic 50.0%
CTATGGTGTGTGCACCAGAA+AGG - chr2_1:65066240-65066259 None:intergenic 50.0%
! CTTTCTGGTGCACACACCAT+AGG + chr2_1:65066238-65066257 Msa0218040:CDS 50.0%
TCCCCATTAGTCCCAGTTAG+AGG - chr2_1:65066583-65066602 None:intergenic 50.0%
! CGCACTATGGTTTGGAGGCT+AGG - chr2_1:65064514-65064533 None:intergenic 55.0%
CCTCCAAACCATAGTGCGCA+GGG + chr2_1:65064516-65064535 Msa0218040:CDS 55.0%
GGTCATTTCCCTGCGCACTA+TGG - chr2_1:65064527-65064546 None:intergenic 55.0%
GCAGCAAACAGCCAAATCCC+TGG + chr2_1:65066142-65066161 Msa0218040:CDS 55.0%
GCCTCCAAACCATAGTGCGC+AGG + chr2_1:65064515-65064534 Msa0218040:CDS 60.0%
! CCCTGCGCACTATGGTTTGG+AGG - chr2_1:65064519-65064538 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 65063972 65066925 65063972 ID=Msa0218040;Name=Msa0218040
chr2_1 mRNA 65063972 65066925 65063972 ID=Msa0218040-mRNA-1;Parent=Msa0218040;Name=Msa0218040-mRNA-1;_AED=0.23;_eAED=0.23;_QI=437|1|1|1|1|1|3|292|318
chr2_1 exon 65063972 65064618 65063972 ID=Msa0218040-mRNA-1:exon:18079;Parent=Msa0218040-mRNA-1
chr2_1 exon 65064703 65064894 65064703 ID=Msa0218040-mRNA-1:exon:18080;Parent=Msa0218040-mRNA-1
chr2_1 exon 65066079 65066925 65066079 ID=Msa0218040-mRNA-1:exon:18081;Parent=Msa0218040-mRNA-1
chr2_1 five_prime_UTR 65063972 65064408 65063972 ID=Msa0218040-mRNA-1:five_prime_utr;Parent=Msa0218040-mRNA-1
chr2_1 CDS 65064409 65064618 65064409 ID=Msa0218040-mRNA-1:cds;Parent=Msa0218040-mRNA-1
chr2_1 CDS 65064703 65064894 65064703 ID=Msa0218040-mRNA-1:cds;Parent=Msa0218040-mRNA-1
chr2_1 CDS 65066079 65066633 65066079 ID=Msa0218040-mRNA-1:cds;Parent=Msa0218040-mRNA-1
chr2_1 three_prime_UTR 65066634 65066925 65066634 ID=Msa0218040-mRNA-1:three_prime_utr;Parent=Msa0218040-mRNA-1
Gene Sequence

>Msa0218040

ATGGCGACCTTAAACAAATTATTTGTTACACTCTCTATTCTTTCTCTCTTTGCTTGTTCAACCAATGCACAACTTAATCCTAACTTCTATGGAAGAACTTGCCCTAGCCTCCAAACCATAGTGCGCAGGGAAATGACCAAAGCTATCAATAATGAAGCTCGAATTGGTGCTTCTATACTTCGATTGTTCTTCCATGATTGCTTTGTAAATGGTTGTGATGGATCAATCTTATTGGATGACACTGCTACCTTTACCGGTGAGAAAAATGCTGGACCCAACAAAAATTCAGCTAGGGGTTTTGAAGTTATTGATACCATTAAAACTAGTGTTGAAGCTGCTTGCAGTGCCACTGTCTCTTGTGCTGATATTCTAGCACTTGCAACTAGAGATGGAATTGCTCTGCTTGGAGGACCAACATGGGTAGTACCACTTGGAAGAAGAGATGCAAGAACAGCAAGTCAAAGTGCAGCAAACAGCCAAATCCCTGGACCATCTTCTGACCTATCCACACTCACTACCATGTTTAGAAACAAAGGTCTAACATTAAATGACCTTACTGTCCTTTCTGGTGCACACACCATAGGTCAAGCAGAATGTCAATTTTTCAGAACTCGTATATACAATGAAACCAACATTGACACAAACTTTGCAACCTTAAGAAAATCAAATTGTCCTACTTCTGGTGGTGACATAAATTTGGCACCTCTTGATTCTGTCTCTCCAGTTACTTTTGACAACAATTACTATAATGACCTTGTTGCTAATAAAGGACTTCTTCATTCTGATCAAGCTCTTTTCAATGGTGTTGGTTCTCAAGTTTCTTTGGTTAGGACTTATAGTAGAAACAATATTGCTTTTAAAAGAGACTTTGCAGCTGCTATGGTGAAGATGAGTAGAATTAGTCCTCTAACTGGGACTAATGGGGAGATTAGAAAGAATTGCAGGCTTGTGAACTAA
Protein sequence

>Msa0218040

MATLNKLFVTLSILSLFACSTNAQLNPNFYGRTCPSLQTIVRREMTKAINNEARIGASILRLFFHDCFVNGCDGSILLDDTATFTGEKNAGPNKNSARGFEVIDTIKTSVEAACSATVSCADILALATRDGIALLGGPTWVVPLGRRDARTASQSAANSQIPGPSSDLSTLTTMFRNKGLTLNDLTVLSGAHTIGQAECQFFRTRIYNETNIDTNFATLRKSNCPTSGGDINLAPLDSVSPVTFDNNYYNDLVANKGLLHSDQALFNGVGSQVSLVRTYSRNNIAFKRDFAAAMVKMSRISPLTGTNGEIRKNCRLVN*