AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0223440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0223440 MtrunA17_Chr5g0442931 100.000 178 0 0 1 178 1 178 2.99e-133 370
Msa0223440 MtrunA17_Chr5g0442901 100.000 178 0 0 1 178 1 178 2.99e-133 370
Msa0223440 MtrunA17_Chr2g0328821 100.000 178 0 0 1 178 17 194 3.33e-133 370
Msa0223440 MtrunA17_Chr3g0095911 94.382 178 10 0 1 178 1 178 2.58e-125 350
Msa0223440 MtrunA17_Chr2g0286131 62.353 85 32 0 92 176 1 85 2.93e-34 117
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0223440 AT2G34480.1 90.230 174 17 0 5 178 5 178 3.01e-119 335
Msa0223440 AT2G34480.2 90.230 174 17 0 5 178 44 217 3.24e-119 336
Msa0223440 AT1G29965.1 87.079 178 23 0 1 178 1 178 1.24e-117 330
Msa0223440 AT3G14600.1 87.079 178 23 0 1 178 1 178 4.20e-117 329
Msa0223440 AT1G29970.2 89.017 173 19 0 6 178 140 312 1.08e-114 328

Find 63 sgRNAs with CRISPR-Local

Find 242 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACCAAGGGGAACTTGATTT+TGG 0.160265 2_1:+73002074 None:intergenic
AGATTAAGAACTATGGAATT+TGG 0.243479 2_1:-73002296 Msa0223440:CDS
GAAAAGTAATGGACAAGTTT+TGG 0.246012 2_1:-73003029 Msa0223440:CDS
GTTGGGCTTCTTAGCTTTGT+AGG 0.272211 2_1:+73002011 None:intergenic
ACTACAATCAGCTCGGAAAA+TGG 0.314844 2_1:-73003387 Msa0223440:exon
ACGAAATGGCATCTCGTCAT+AGG 0.320455 2_1:-73002185 Msa0223440:CDS
AGATTTATCGCATGAAGCTT+TGG 0.333905 2_1:-73003204 Msa0223440:CDS
GATTTATCGCATGAAGCTTT+GGG 0.349457 2_1:-73003203 Msa0223440:CDS
GTCAGTACTTTAAGAGCTTT+CGG 0.352974 2_1:-73001927 Msa0223440:three_prime_UTR
TGTTTGTGCACTCTTATTGC+TGG 0.354017 2_1:-73001703 Msa0223440:three_prime_UTR
CCTACCAAGATTAAGAACTA+TGG 0.366976 2_1:-73002303 Msa0223440:CDS
AACTTACCAGAACTTGGATT+TGG 0.367421 2_1:+73003162 None:intergenic
CCTTGGTGTACAAGAAGATT+AGG 0.391832 2_1:-73002059 Msa0223440:CDS
TCCCAGCTAATCTGTGCAAA+AGG 0.391912 2_1:-73002122 Msa0223440:CDS
AATTCCATAGTTCTTAATCT+TGG 0.403701 2_1:+73002299 None:intergenic
ATTCACTGAATCACATAAAC+AGG 0.404006 2_1:+73001989 None:intergenic
AGCTTCATGCGATAAATCTT+AGG 0.407205 2_1:+73003208 None:intergenic
CTGTCTGAGTTTGCTGGTAA+TGG 0.414348 2_1:+73001880 None:intergenic
TGGTGGCGGTCTTAATGATC+TGG 0.424945 2_1:+73002145 None:intergenic
TACCGTGATACTACTCTAAA+CGG 0.448863 2_1:-73002228 Msa0223440:CDS
ATAAGGAACTTACCAGAACT+TGG 0.449276 2_1:+73003156 None:intergenic
ACAAAATACTTGATAAATAA+CGG 0.456124 2_1:+73001838 None:intergenic
TTCGTGCCAAATCCAAGTTC+TGG 0.468779 2_1:-73003168 Msa0223440:intron
CGAACACTGTCTGAGTTTGC+TGG 0.479837 2_1:+73001874 None:intergenic
CTGAATCACATAAACAGGTT+GGG 0.486125 2_1:+73001994 None:intergenic
CTCTTCCGACAACCTGGTAC+TGG 0.487827 2_1:+73003257 None:intergenic
TTTCTTGAGGAAGTTGAAGA+AGG 0.490067 2_1:-73003056 Msa0223440:CDS
TGGCATCTCGTCATAGGGTC+AGG 0.499078 2_1:-73002179 Msa0223440:CDS
TTGCGTTATCAGAGTCGTAC+TGG 0.505845 2_1:-73002273 Msa0223440:CDS
AGAAGATTAGGCCTCCTACT+AGG 0.511127 2_1:-73002047 Msa0223440:CDS
TCCACAGTTTCACCAGTACC+AGG 0.511898 2_1:-73003269 Msa0223440:intron
TTGATGTACCGGTACAAGCT+TGG 0.513924 2_1:-73001777 Msa0223440:three_prime_UTR
AAGAAGGTTAAGAAAAGTAA+TGG 0.518066 2_1:-73003040 Msa0223440:CDS
TTTGCACAGATTAGCTGGGA+TGG 0.520373 2_1:+73002125 None:intergenic
GGAGACAATGTACAACGAAA+TGG 0.522780 2_1:-73002199 Msa0223440:CDS
CAAGCAACTCTAACATGAGA+AGG 0.524095 2_1:+73001958 None:intergenic
CCCAGCTAATCTGTGCAAAA+GGG 0.524734 2_1:-73002121 Msa0223440:CDS
AAGAGCACTTCCCACCGAAA+AGG 0.530461 2_1:-73003239 Msa0223440:CDS
TTTCACCAGTACCAGGTTGT+CGG 0.538716 2_1:-73003262 Msa0223440:CDS
CGAAATGGCATCTCGTCATA+GGG 0.541059 2_1:-73002184 Msa0223440:CDS
CCATAGTTCTTAATCTTGGT+AGG 0.548600 2_1:+73002303 None:intergenic
TCGGAAAATGGTTAACGCTA+AGG 0.552440 2_1:-73003375 Msa0223440:intron
TTCCAAAATCAAGTTCCCCT+TGG 0.555039 2_1:-73002076 Msa0223440:CDS
GGATTTGGCACGAACCTCGT+TGG 0.555937 2_1:+73003177 None:intergenic
CAGATTAGCTGGGATGGTGG+CGG 0.559030 2_1:+73002131 None:intergenic
ACTGAATCACATAAACAGGT+TGG 0.563649 2_1:+73001993 None:intergenic
ATGCTAATAGCTAATGAGCT+AGG 0.567864 2_1:-73001664 Msa0223440:three_prime_UTR
ATTCAAAACCAAGCTTGTAC+CGG 0.570756 2_1:+73001769 None:intergenic
CTAATCTTCTTGTACACCAA+GGG 0.571190 2_1:+73002060 None:intergenic
GTCTTAATGATCTGGATGCA+AGG 0.582689 2_1:+73002153 None:intergenic
GAAGTGCTCTTCCGACAACC+TGG 0.588662 2_1:+73003251 None:intergenic
GCACAGATTAGCTGGGATGG+TGG 0.592241 2_1:+73002128 None:intergenic
AAGCAACTCTAACATGAGAA+GGG 0.603748 2_1:+73001959 None:intergenic
GCTGATGAACGAAGCTGTGT+TGG 0.604991 2_1:-73001633 Msa0223440:three_prime_UTR
TGGTTATCACAATATGTACA+AGG 0.613559 2_1:-73002253 Msa0223440:CDS
ACAGAAAACTACAATCAGCT+CGG 0.620129 2_1:-73003394 Msa0223440:five_prime_UTR
CCTAATCTTCTTGTACACCA+AGG 0.642782 2_1:+73002059 None:intergenic
TACTACTCTAAACGGTGCTG+TGG 0.650976 2_1:-73002220 Msa0223440:CDS
ATTGTTGTAGGTATTTCTTG+AGG 0.651477 2_1:-73003069 Msa0223440:intron
CACCGTTTAGAGTAGTATCA+CGG 0.660256 2_1:+73002226 None:intergenic
TACTTGATAAATAACGGACT+AGG 0.668215 2_1:+73001844 None:intergenic
TAATCTTCTTGTACACCAAG+GGG 0.679937 2_1:+73002061 None:intergenic
GAAGCTTTGGGCAACCAACG+AGG 0.727612 2_1:-73003191 Msa0223440:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATAATGTTAATTGTTGT+AGG - chr2_1:73001971-73001990 Msa0223440:three_prime_UTR 15.0%
!! ACAAAATACTTGATAAATAA+CGG + chr2_1:73003217-73003236 None:intergenic 15.0%
!! TTAATAATGTTAATTGTTGT+AGG - chr2_1:73001971-73001990 Msa0223440:three_prime_UTR 15.0%
!! ACAAAATACTTGATAAATAA+CGG + chr2_1:73003217-73003236 None:intergenic 15.0%
!! TGTATCAAATTTGAATTAAG+AGG + chr2_1:73002082-73002101 None:intergenic 20.0%
!! ATTCAAATTTGATACAACTT+AGG - chr2_1:73002086-73002105 Msa0223440:CDS 20.0%
!! AAATCATTGAAATGTCATAT+AGG + chr2_1:73002203-73002222 None:intergenic 20.0%
!! TTGTTGTAACATATCTATTA+AGG - chr2_1:73002428-73002447 Msa0223440:intron 20.0%
!! CGTATTGTTTCAATTATTAT+AGG - chr2_1:73002580-73002599 Msa0223440:intron 20.0%
!! CAATTATTATAGGCAAAATA+AGG - chr2_1:73002590-73002609 Msa0223440:intron 20.0%
!! TGTATCAAATTTGAATTAAG+AGG + chr2_1:73002082-73002101 None:intergenic 20.0%
!! ATTCAAATTTGATACAACTT+AGG - chr2_1:73002086-73002105 Msa0223440:CDS 20.0%
!! AAATCATTGAAATGTCATAT+AGG + chr2_1:73002203-73002222 None:intergenic 20.0%
!! TTGTTGTAACATATCTATTA+AGG - chr2_1:73002428-73002447 Msa0223440:intron 20.0%
!! CGTATTGTTTCAATTATTAT+AGG - chr2_1:73002580-73002599 Msa0223440:intron 20.0%
!! CAATTATTATAGGCAAAATA+AGG - chr2_1:73002590-73002609 Msa0223440:intron 20.0%
! AAGAAGGTTAAGAAAAGTAA+TGG - chr2_1:73002012-73002031 Msa0223440:CDS 25.0%
! TTATGTCAAAATCCAAACAT+AGG - chr2_1:73002398-73002417 Msa0223440:intron 25.0%
! AATAGATATGTTACAACAAC+AGG + chr2_1:73002427-73002446 None:intergenic 25.0%
! AATTCCATAGTTCTTAATCT+TGG + chr2_1:73002756-73002775 None:intergenic 25.0%
! AGATTAAGAACTATGGAATT+TGG - chr2_1:73002756-73002775 Msa0223440:intron 25.0%
!! TTTTTCTCTTTTTGATGTAC+CGG - chr2_1:73003264-73003283 Msa0223440:CDS 25.0%
!!! TAGTGAACTTTTTGTTTTGA+AGG - chr2_1:73003307-73003326 Msa0223440:intron 25.0%
! AAGAAGGTTAAGAAAAGTAA+TGG - chr2_1:73002012-73002031 Msa0223440:CDS 25.0%
! TTATGTCAAAATCCAAACAT+AGG - chr2_1:73002398-73002417 Msa0223440:intron 25.0%
! AATAGATATGTTACAACAAC+AGG + chr2_1:73002427-73002446 None:intergenic 25.0%
! AATTCCATAGTTCTTAATCT+TGG + chr2_1:73002756-73002775 None:intergenic 25.0%
! AGATTAAGAACTATGGAATT+TGG - chr2_1:73002756-73002775 Msa0223440:intron 25.0%
!! TTTTTCTCTTTTTGATGTAC+CGG - chr2_1:73003264-73003283 Msa0223440:CDS 25.0%
!!! TAGTGAACTTTTTGTTTTGA+AGG - chr2_1:73003307-73003326 Msa0223440:intron 25.0%
TTTGTGTGTGAATCTTAACA+CGG - chr2_1:73001745-73001764 Msa0223440:three_prime_UTR 30.0%
ATTGTTGTAGGTATTTCTTG+AGG - chr2_1:73001983-73002002 Msa0223440:exon 30.0%
!!! GAAAAGTAATGGACAAGTTT+TGG - chr2_1:73002023-73002042 Msa0223440:CDS 30.0%
TCATAAACTGTATCATGGAA+TGG + chr2_1:73002242-73002261 None:intergenic 30.0%
ACAAGTCATAAACTGTATCA+TGG + chr2_1:73002247-73002266 None:intergenic 30.0%
!! TGACTTGTTTGAAAGTACTT+TGG - chr2_1:73002259-73002278 Msa0223440:CDS 30.0%
!! TTGTTTGAAAGTACTTTGGT+TGG - chr2_1:73002263-73002282 Msa0223440:CDS 30.0%
!! TGTTTGAAAGTACTTTGGTT+GGG - chr2_1:73002264-73002283 Msa0223440:CDS 30.0%
AATTGTGGTGAAAGAATATG+TGG - chr2_1:73002615-73002634 Msa0223440:intron 30.0%
TAACAACTAAAGCAAACACA+GGG + chr2_1:73002651-73002670 None:intergenic 30.0%
ATAACAACTAAAGCAAACAC+AGG + chr2_1:73002652-73002671 None:intergenic 30.0%
TGGTTATCACAATATGTACA+AGG - chr2_1:73002799-73002818 Msa0223440:intron 30.0%
!!! TAGCTTTGTAGGTTGTTTTT+AGG + chr2_1:73003033-73003052 None:intergenic 30.0%
ATTCACTGAATCACATAAAC+AGG + chr2_1:73003066-73003085 None:intergenic 30.0%
!! TACTTGATAAATAACGGACT+AGG + chr2_1:73003211-73003230 None:intergenic 30.0%
!!! TGAACTTTTTGTTTTGAAGG+AGG - chr2_1:73003310-73003329 Msa0223440:intron 30.0%
!!! GAACTTTTTGTTTTGAAGGA+GGG - chr2_1:73003311-73003330 Msa0223440:intron 30.0%
TTTGTGTGTGAATCTTAACA+CGG - chr2_1:73001745-73001764 Msa0223440:three_prime_UTR 30.0%
ATTGTTGTAGGTATTTCTTG+AGG - chr2_1:73001983-73002002 Msa0223440:exon 30.0%
!!! GAAAAGTAATGGACAAGTTT+TGG - chr2_1:73002023-73002042 Msa0223440:CDS 30.0%
TCATAAACTGTATCATGGAA+TGG + chr2_1:73002242-73002261 None:intergenic 30.0%
ACAAGTCATAAACTGTATCA+TGG + chr2_1:73002247-73002266 None:intergenic 30.0%
!! TGACTTGTTTGAAAGTACTT+TGG - chr2_1:73002259-73002278 Msa0223440:CDS 30.0%
!! TTGTTTGAAAGTACTTTGGT+TGG - chr2_1:73002263-73002282 Msa0223440:CDS 30.0%
!! TGTTTGAAAGTACTTTGGTT+GGG - chr2_1:73002264-73002283 Msa0223440:CDS 30.0%
AATTGTGGTGAAAGAATATG+TGG - chr2_1:73002615-73002634 Msa0223440:intron 30.0%
TAACAACTAAAGCAAACACA+GGG + chr2_1:73002651-73002670 None:intergenic 30.0%
ATAACAACTAAAGCAAACAC+AGG + chr2_1:73002652-73002671 None:intergenic 30.0%
TGGTTATCACAATATGTACA+AGG - chr2_1:73002799-73002818 Msa0223440:intron 30.0%
!!! TAGCTTTGTAGGTTGTTTTT+AGG + chr2_1:73003033-73003052 None:intergenic 30.0%
ATTCACTGAATCACATAAAC+AGG + chr2_1:73003066-73003085 None:intergenic 30.0%
!! TACTTGATAAATAACGGACT+AGG + chr2_1:73003211-73003230 None:intergenic 30.0%
!!! TGAACTTTTTGTTTTGAAGG+AGG - chr2_1:73003310-73003329 Msa0223440:intron 30.0%
!!! GAACTTTTTGTTTTGAAGGA+GGG - chr2_1:73003311-73003330 Msa0223440:intron 30.0%
ACAGAAAACTACAATCAGCT+CGG - chr2_1:73001658-73001677 Msa0223440:three_prime_UTR 35.0%
! CTTAGGATGTTCATCCTTTT+CGG + chr2_1:73001830-73001849 None:intergenic 35.0%
AGCTTCATGCGATAAATCTT+AGG + chr2_1:73001847-73001866 None:intergenic 35.0%
AGATTTATCGCATGAAGCTT+TGG - chr2_1:73001848-73001867 Msa0223440:three_prime_UTR 35.0%
GATTTATCGCATGAAGCTTT+GGG - chr2_1:73001849-73001868 Msa0223440:three_prime_UTR 35.0%
AACTTACCAGAACTTGGATT+TGG + chr2_1:73001893-73001912 None:intergenic 35.0%
ATAAGGAACTTACCAGAACT+TGG + chr2_1:73001899-73001918 None:intergenic 35.0%
TTTCTTGAGGAAGTTGAAGA+AGG - chr2_1:73001996-73002015 Msa0223440:exon 35.0%
!!! ACAAGTTTTGGCTATCAATG+AGG - chr2_1:73002035-73002054 Msa0223440:CDS 35.0%
! AAAGTACTTTGGTTGGGAAA+GGG - chr2_1:73002270-73002289 Msa0223440:CDS 35.0%
!!! TGTTCTGTTTTGATTTGTGC+TGG - chr2_1:73002295-73002314 Msa0223440:CDS 35.0%
!!! GTTCTGTTTTGATTTGTGCT+GGG - chr2_1:73002296-73002315 Msa0223440:CDS 35.0%
ACAGCTTACACTCAAATGTT+TGG + chr2_1:73002362-73002381 None:intergenic 35.0%
AACAACAGGTAACCTATGTT+TGG + chr2_1:73002413-73002432 None:intergenic 35.0%
TGTCTTGAGTATTTCCTAGA+GGG - chr2_1:73002491-73002510 Msa0223440:intron 35.0%
TCAGCTATGAAATTAGTCAC+TGG + chr2_1:73002530-73002549 None:intergenic 35.0%
AGGCAAAATAAGGCTAATTG+TGG - chr2_1:73002600-73002619 Msa0223440:intron 35.0%
! ACCTTCAATTGCAGTCTTTT+TGG - chr2_1:73002675-73002694 Msa0223440:intron 35.0%
TCCAAAAAGACTGCAATTGA+AGG + chr2_1:73002679-73002698 None:intergenic 35.0%
! ATTGCAGTCTTTTTGGACAT+AGG - chr2_1:73002682-73002701 Msa0223440:intron 35.0%
! TTGCAGTCTTTTTGGACATA+GGG - chr2_1:73002683-73002702 Msa0223440:intron 35.0%
CCATAGTTCTTAATCTTGGT+AGG + chr2_1:73002752-73002771 None:intergenic 35.0%
CCTACCAAGATTAAGAACTA+TGG - chr2_1:73002749-73002768 Msa0223440:intron 35.0%
TACCGTGATACTACTCTAAA+CGG - chr2_1:73002824-73002843 Msa0223440:intron 35.0%
!!! GGAACTTGATTTTGGAATTG+TGG + chr2_1:73002973-73002992 None:intergenic 35.0%
TAATCTTCTTGTACACCAAG+GGG + chr2_1:73002994-73003013 None:intergenic 35.0%
CTAATCTTCTTGTACACCAA+GGG + chr2_1:73002995-73003014 None:intergenic 35.0%
CTGAATCACATAAACAGGTT+GGG + chr2_1:73003061-73003080 None:intergenic 35.0%
ACTGAATCACATAAACAGGT+TGG + chr2_1:73003062-73003081 None:intergenic 35.0%
AAGCAACTCTAACATGAGAA+GGG + chr2_1:73003096-73003115 None:intergenic 35.0%
! GTCAGTACTTTAAGAGCTTT+CGG - chr2_1:73003125-73003144 Msa0223440:intron 35.0%
ATTCAAAACCAAGCTTGTAC+CGG + chr2_1:73003286-73003305 None:intergenic 35.0%
ATGCTAATAGCTAATGAGCT+AGG - chr2_1:73003388-73003407 Msa0223440:exon 35.0%
ACAGAAAACTACAATCAGCT+CGG - chr2_1:73001658-73001677 Msa0223440:three_prime_UTR 35.0%
! CTTAGGATGTTCATCCTTTT+CGG + chr2_1:73001830-73001849 None:intergenic 35.0%
AGCTTCATGCGATAAATCTT+AGG + chr2_1:73001847-73001866 None:intergenic 35.0%
AGATTTATCGCATGAAGCTT+TGG - chr2_1:73001848-73001867 Msa0223440:three_prime_UTR 35.0%
GATTTATCGCATGAAGCTTT+GGG - chr2_1:73001849-73001868 Msa0223440:three_prime_UTR 35.0%
AACTTACCAGAACTTGGATT+TGG + chr2_1:73001893-73001912 None:intergenic 35.0%
ATAAGGAACTTACCAGAACT+TGG + chr2_1:73001899-73001918 None:intergenic 35.0%
TTTCTTGAGGAAGTTGAAGA+AGG - chr2_1:73001996-73002015 Msa0223440:exon 35.0%
!!! ACAAGTTTTGGCTATCAATG+AGG - chr2_1:73002035-73002054 Msa0223440:CDS 35.0%
! AAAGTACTTTGGTTGGGAAA+GGG - chr2_1:73002270-73002289 Msa0223440:CDS 35.0%
!!! TGTTCTGTTTTGATTTGTGC+TGG - chr2_1:73002295-73002314 Msa0223440:CDS 35.0%
!!! GTTCTGTTTTGATTTGTGCT+GGG - chr2_1:73002296-73002315 Msa0223440:CDS 35.0%
ACAGCTTACACTCAAATGTT+TGG + chr2_1:73002362-73002381 None:intergenic 35.0%
AACAACAGGTAACCTATGTT+TGG + chr2_1:73002413-73002432 None:intergenic 35.0%
TGTCTTGAGTATTTCCTAGA+GGG - chr2_1:73002491-73002510 Msa0223440:intron 35.0%
TCAGCTATGAAATTAGTCAC+TGG + chr2_1:73002530-73002549 None:intergenic 35.0%
AGGCAAAATAAGGCTAATTG+TGG - chr2_1:73002600-73002619 Msa0223440:intron 35.0%
! ACCTTCAATTGCAGTCTTTT+TGG - chr2_1:73002675-73002694 Msa0223440:intron 35.0%
TCCAAAAAGACTGCAATTGA+AGG + chr2_1:73002679-73002698 None:intergenic 35.0%
! ATTGCAGTCTTTTTGGACAT+AGG - chr2_1:73002682-73002701 Msa0223440:intron 35.0%
! TTGCAGTCTTTTTGGACATA+GGG - chr2_1:73002683-73002702 Msa0223440:intron 35.0%
CCATAGTTCTTAATCTTGGT+AGG + chr2_1:73002752-73002771 None:intergenic 35.0%
CCTACCAAGATTAAGAACTA+TGG - chr2_1:73002749-73002768 Msa0223440:intron 35.0%
TACCGTGATACTACTCTAAA+CGG - chr2_1:73002824-73002843 Msa0223440:intron 35.0%
!!! GGAACTTGATTTTGGAATTG+TGG + chr2_1:73002973-73002992 None:intergenic 35.0%
TAATCTTCTTGTACACCAAG+GGG + chr2_1:73002994-73003013 None:intergenic 35.0%
CTAATCTTCTTGTACACCAA+GGG + chr2_1:73002995-73003014 None:intergenic 35.0%
CTGAATCACATAAACAGGTT+GGG + chr2_1:73003061-73003080 None:intergenic 35.0%
ACTGAATCACATAAACAGGT+TGG + chr2_1:73003062-73003081 None:intergenic 35.0%
AAGCAACTCTAACATGAGAA+GGG + chr2_1:73003096-73003115 None:intergenic 35.0%
! GTCAGTACTTTAAGAGCTTT+CGG - chr2_1:73003125-73003144 Msa0223440:intron 35.0%
ATTCAAAACCAAGCTTGTAC+CGG + chr2_1:73003286-73003305 None:intergenic 35.0%
ATGCTAATAGCTAATGAGCT+AGG - chr2_1:73003388-73003407 Msa0223440:exon 35.0%
ACTACAATCAGCTCGGAAAA+TGG - chr2_1:73001665-73001684 Msa0223440:three_prime_UTR 40.0%
TCGGAAAATGGTTAACGCTA+AGG - chr2_1:73001677-73001696 Msa0223440:three_prime_UTR 40.0%
GATACAACTTAGGCTGTGTT+TGG - chr2_1:73002096-73002115 Msa0223440:CDS 40.0%
ATACCGAACAAGTGCTTCTA+GGG + chr2_1:73002158-73002177 None:intergenic 40.0%
AATACCGAACAAGTGCTTCT+AGG + chr2_1:73002159-73002178 None:intergenic 40.0%
!! GAAAGTACTTTGGTTGGGAA+AGG - chr2_1:73002269-73002288 Msa0223440:CDS 40.0%
GGCATACAGTAACAGTTTGA+AGG + chr2_1:73002471-73002490 None:intergenic 40.0%
! CTCTAGGAAATACTCAAGAC+AGG + chr2_1:73002492-73002511 None:intergenic 40.0%
CTGTCTTGAGTATTTCCTAG+AGG - chr2_1:73002490-73002509 Msa0223440:intron 40.0%
GTTTCATGCTAAAACCCTCT+AGG + chr2_1:73002508-73002527 None:intergenic 40.0%
! TGCAGTCTTTTTGGACATAG+GGG - chr2_1:73002684-73002703 Msa0223440:intron 40.0%
! TTTTTGGACATAGGGGCTTA+TGG - chr2_1:73002691-73002710 Msa0223440:intron 40.0%
! CACCGTTTAGAGTAGTATCA+CGG + chr2_1:73002829-73002848 None:intergenic 40.0%
GGAGACAATGTACAACGAAA+TGG - chr2_1:73002853-73002872 Msa0223440:intron 40.0%
GTCTTAATGATCTGGATGCA+AGG + chr2_1:73002902-73002921 None:intergenic 40.0%
TTCCAAAATCAAGTTCCCCT+TGG - chr2_1:73002976-73002995 Msa0223440:intron 40.0%
CCTAATCTTCTTGTACACCA+AGG + chr2_1:73002996-73003015 None:intergenic 40.0%
! CCTTGGTGTACAAGAAGATT+AGG - chr2_1:73002993-73003012 Msa0223440:intron 40.0%
!!! GTTGTTTTTAGGCTCCTAGT+AGG + chr2_1:73003022-73003041 None:intergenic 40.0%
CAAGCAACTCTAACATGAGA+AGG + chr2_1:73003097-73003116 None:intergenic 40.0%
TGTTTGTGCACTCTTATTGC+TGG - chr2_1:73003349-73003368 Msa0223440:intron 40.0%
ACTACAATCAGCTCGGAAAA+TGG - chr2_1:73001665-73001684 Msa0223440:three_prime_UTR 40.0%
TCGGAAAATGGTTAACGCTA+AGG - chr2_1:73001677-73001696 Msa0223440:three_prime_UTR 40.0%
GATACAACTTAGGCTGTGTT+TGG - chr2_1:73002096-73002115 Msa0223440:CDS 40.0%
ATACCGAACAAGTGCTTCTA+GGG + chr2_1:73002158-73002177 None:intergenic 40.0%
AATACCGAACAAGTGCTTCT+AGG + chr2_1:73002159-73002178 None:intergenic 40.0%
!! GAAAGTACTTTGGTTGGGAA+AGG - chr2_1:73002269-73002288 Msa0223440:CDS 40.0%
GGCATACAGTAACAGTTTGA+AGG + chr2_1:73002471-73002490 None:intergenic 40.0%
! CTCTAGGAAATACTCAAGAC+AGG + chr2_1:73002492-73002511 None:intergenic 40.0%
CTGTCTTGAGTATTTCCTAG+AGG - chr2_1:73002490-73002509 Msa0223440:intron 40.0%
GTTTCATGCTAAAACCCTCT+AGG + chr2_1:73002508-73002527 None:intergenic 40.0%
! TGCAGTCTTTTTGGACATAG+GGG - chr2_1:73002684-73002703 Msa0223440:intron 40.0%
! TTTTTGGACATAGGGGCTTA+TGG - chr2_1:73002691-73002710 Msa0223440:intron 40.0%
! CACCGTTTAGAGTAGTATCA+CGG + chr2_1:73002829-73002848 None:intergenic 40.0%
GGAGACAATGTACAACGAAA+TGG - chr2_1:73002853-73002872 Msa0223440:intron 40.0%
GTCTTAATGATCTGGATGCA+AGG + chr2_1:73002902-73002921 None:intergenic 40.0%
TTCCAAAATCAAGTTCCCCT+TGG - chr2_1:73002976-73002995 Msa0223440:intron 40.0%
CCTAATCTTCTTGTACACCA+AGG + chr2_1:73002996-73003015 None:intergenic 40.0%
! CCTTGGTGTACAAGAAGATT+AGG - chr2_1:73002993-73003012 Msa0223440:intron 40.0%
!!! GTTGTTTTTAGGCTCCTAGT+AGG + chr2_1:73003022-73003041 None:intergenic 40.0%
CAAGCAACTCTAACATGAGA+AGG + chr2_1:73003097-73003116 None:intergenic 40.0%
TGTTTGTGCACTCTTATTGC+TGG - chr2_1:73003349-73003368 Msa0223440:intron 40.0%
TTTCACCAGTACCAGGTTGT+CGG - chr2_1:73001790-73001809 Msa0223440:three_prime_UTR 45.0%
! GGATGTTCATCCTTTTCGGT+GGG + chr2_1:73001826-73001845 None:intergenic 45.0%
! AGGATGTTCATCCTTTTCGG+TGG + chr2_1:73001827-73001846 None:intergenic 45.0%
TTCGTGCCAAATCCAAGTTC+TGG - chr2_1:73001884-73001903 Msa0223440:three_prime_UTR 45.0%
TACCGAACAAGTGCTTCTAG+GGG + chr2_1:73002157-73002176 None:intergenic 45.0%
TTGCGTTATCAGAGTCGTAC+TGG - chr2_1:73002779-73002798 Msa0223440:intron 45.0%
! TACTACTCTAAACGGTGCTG+TGG - chr2_1:73002832-73002851 Msa0223440:intron 45.0%
ACGAAATGGCATCTCGTCAT+AGG - chr2_1:73002867-73002886 Msa0223440:intron 45.0%
CGAAATGGCATCTCGTCATA+GGG - chr2_1:73002868-73002887 Msa0223440:intron 45.0%
! TTTGCACAGATTAGCTGGGA+TGG + chr2_1:73002930-73002949 None:intergenic 45.0%
TCCCAGCTAATCTGTGCAAA+AGG - chr2_1:73002930-73002949 Msa0223440:intron 45.0%
!! CCCTTTTGCACAGATTAGCT+GGG + chr2_1:73002934-73002953 None:intergenic 45.0%
CCCAGCTAATCTGTGCAAAA+GGG - chr2_1:73002931-73002950 Msa0223440:intron 45.0%
! TCCCTTTTGCACAGATTAGC+TGG + chr2_1:73002935-73002954 None:intergenic 45.0%
! CACCAAGGGGAACTTGATTT+TGG + chr2_1:73002981-73003000 None:intergenic 45.0%
AGAAGATTAGGCCTCCTACT+AGG - chr2_1:73003005-73003024 Msa0223440:intron 45.0%
!! GTTTTTAGGCTCCTAGTAGG+AGG + chr2_1:73003019-73003038 None:intergenic 45.0%
!! GTTGGGCTTCTTAGCTTTGT+AGG + chr2_1:73003044-73003063 None:intergenic 45.0%
!! CTGTCTGAGTTTGCTGGTAA+TGG + chr2_1:73003175-73003194 None:intergenic 45.0%
! TTGATGTACCGGTACAAGCT+TGG - chr2_1:73003275-73003294 Msa0223440:intron 45.0%
TTTCACCAGTACCAGGTTGT+CGG - chr2_1:73001790-73001809 Msa0223440:three_prime_UTR 45.0%
! GGATGTTCATCCTTTTCGGT+GGG + chr2_1:73001826-73001845 None:intergenic 45.0%
! AGGATGTTCATCCTTTTCGG+TGG + chr2_1:73001827-73001846 None:intergenic 45.0%
TTCGTGCCAAATCCAAGTTC+TGG - chr2_1:73001884-73001903 Msa0223440:three_prime_UTR 45.0%
TACCGAACAAGTGCTTCTAG+GGG + chr2_1:73002157-73002176 None:intergenic 45.0%
TTGCGTTATCAGAGTCGTAC+TGG - chr2_1:73002779-73002798 Msa0223440:intron 45.0%
! TACTACTCTAAACGGTGCTG+TGG - chr2_1:73002832-73002851 Msa0223440:intron 45.0%
ACGAAATGGCATCTCGTCAT+AGG - chr2_1:73002867-73002886 Msa0223440:intron 45.0%
CGAAATGGCATCTCGTCATA+GGG - chr2_1:73002868-73002887 Msa0223440:intron 45.0%
! TTTGCACAGATTAGCTGGGA+TGG + chr2_1:73002930-73002949 None:intergenic 45.0%
TCCCAGCTAATCTGTGCAAA+AGG - chr2_1:73002930-73002949 Msa0223440:intron 45.0%
!! CCCTTTTGCACAGATTAGCT+GGG + chr2_1:73002934-73002953 None:intergenic 45.0%
CCCAGCTAATCTGTGCAAAA+GGG - chr2_1:73002931-73002950 Msa0223440:intron 45.0%
! TCCCTTTTGCACAGATTAGC+TGG + chr2_1:73002935-73002954 None:intergenic 45.0%
! CACCAAGGGGAACTTGATTT+TGG + chr2_1:73002981-73003000 None:intergenic 45.0%
AGAAGATTAGGCCTCCTACT+AGG - chr2_1:73003005-73003024 Msa0223440:intron 45.0%
!! GTTTTTAGGCTCCTAGTAGG+AGG + chr2_1:73003019-73003038 None:intergenic 45.0%
!! GTTGGGCTTCTTAGCTTTGT+AGG + chr2_1:73003044-73003063 None:intergenic 45.0%
!! CTGTCTGAGTTTGCTGGTAA+TGG + chr2_1:73003175-73003194 None:intergenic 45.0%
! TTGATGTACCGGTACAAGCT+TGG - chr2_1:73003275-73003294 Msa0223440:intron 45.0%
!! TGTTAATTAATAAATAAATA+AGG + chr2_1:73001916-73001935 None:intergenic 5.0%
!! TGTTAATTAATAAATAAATA+AGG + chr2_1:73001916-73001935 None:intergenic 5.0%
TCCACAGTTTCACCAGTACC+AGG - chr2_1:73001783-73001802 Msa0223440:three_prime_UTR 50.0%
!! ACCTGGTACTGGTGAAACTG+TGG + chr2_1:73001787-73001806 None:intergenic 50.0%
!! AAGAGCACTTCCCACCGAAA+AGG - chr2_1:73001813-73001832 Msa0223440:three_prime_UTR 50.0%
!! GACCCCTAGAAGCACTTGTT+CGG - chr2_1:73002152-73002171 Msa0223440:CDS 50.0%
!! TGGTGGCGGTCTTAATGATC+TGG + chr2_1:73002910-73002929 None:intergenic 50.0%
CGAACACTGTCTGAGTTTGC+TGG + chr2_1:73003181-73003200 None:intergenic 50.0%
GCTGATGAACGAAGCTGTGT+TGG - chr2_1:73003419-73003438 Msa0223440:five_prime_UTR 50.0%
TCCACAGTTTCACCAGTACC+AGG - chr2_1:73001783-73001802 Msa0223440:three_prime_UTR 50.0%
!! ACCTGGTACTGGTGAAACTG+TGG + chr2_1:73001787-73001806 None:intergenic 50.0%
!! AAGAGCACTTCCCACCGAAA+AGG - chr2_1:73001813-73001832 Msa0223440:three_prime_UTR 50.0%
!! GACCCCTAGAAGCACTTGTT+CGG - chr2_1:73002152-73002171 Msa0223440:CDS 50.0%
!! TGGTGGCGGTCTTAATGATC+TGG + chr2_1:73002910-73002929 None:intergenic 50.0%
CGAACACTGTCTGAGTTTGC+TGG + chr2_1:73003181-73003200 None:intergenic 50.0%
GCTGATGAACGAAGCTGTGT+TGG - chr2_1:73003419-73003438 Msa0223440:five_prime_UTR 50.0%
CTCTTCCGACAACCTGGTAC+TGG + chr2_1:73001798-73001817 None:intergenic 55.0%
! GAAGTGCTCTTCCGACAACC+TGG + chr2_1:73001804-73001823 None:intergenic 55.0%
!! GAAGCTTTGGGCAACCAACG+AGG - chr2_1:73001861-73001880 Msa0223440:three_prime_UTR 55.0%
GGATTTGGCACGAACCTCGT+TGG + chr2_1:73001878-73001897 None:intergenic 55.0%
TGGCATCTCGTCATAGGGTC+AGG - chr2_1:73002873-73002892 Msa0223440:intron 55.0%
! CAGATTAGCTGGGATGGTGG+CGG + chr2_1:73002924-73002943 None:intergenic 55.0%
! GCACAGATTAGCTGGGATGG+TGG + chr2_1:73002927-73002946 None:intergenic 55.0%
CTCTTCCGACAACCTGGTAC+TGG + chr2_1:73001798-73001817 None:intergenic 55.0%
! GAAGTGCTCTTCCGACAACC+TGG + chr2_1:73001804-73001823 None:intergenic 55.0%
!! GAAGCTTTGGGCAACCAACG+AGG - chr2_1:73001861-73001880 Msa0223440:three_prime_UTR 55.0%
GGATTTGGCACGAACCTCGT+TGG + chr2_1:73001878-73001897 None:intergenic 55.0%
TGGCATCTCGTCATAGGGTC+AGG - chr2_1:73002873-73002892 Msa0223440:intron 55.0%
! CAGATTAGCTGGGATGGTGG+CGG + chr2_1:73002924-73002943 None:intergenic 55.0%
! GCACAGATTAGCTGGGATGG+TGG + chr2_1:73002927-73002946 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 73001628 73003446 73001628 ID=Msa0223440;Name=Msa0223440
chr2_1 mRNA 73001628 73003446 73001628 ID=Msa0223440-mRNA-1;Parent=Msa0223440;Name=Msa0223440-mRNA-1;_AED=0.05;_eAED=0.05;_QI=56|1|1|1|1|1|4|371|178
chr2_1 exon 73001628 73002340 73001628 ID=Msa0223440-mRNA-1:exon:21283;Parent=Msa0223440-mRNA-1
chr2_1 exon 73003018 73003081 73003018 ID=Msa0223440-mRNA-1:exon:21282;Parent=Msa0223440-mRNA-1
chr2_1 exon 73003169 73003284 73003169 ID=Msa0223440-mRNA-1:exon:21281;Parent=Msa0223440-mRNA-1
chr2_1 exon 73003376 73003446 73003376 ID=Msa0223440-mRNA-1:exon:21280;Parent=Msa0223440-mRNA-1
chr2_1 five_prime_UTR 73003391 73003446 73003391 ID=Msa0223440-mRNA-1:five_prime_utr;Parent=Msa0223440-mRNA-1
chr2_1 CDS 73003376 73003390 73003376 ID=Msa0223440-mRNA-1:cds;Parent=Msa0223440-mRNA-1
chr2_1 CDS 73003169 73003284 73003169 ID=Msa0223440-mRNA-1:cds;Parent=Msa0223440-mRNA-1
chr2_1 CDS 73003018 73003081 73003018 ID=Msa0223440-mRNA-1:cds;Parent=Msa0223440-mRNA-1
chr2_1 CDS 73001999 73002340 73001999 ID=Msa0223440-mRNA-1:cds;Parent=Msa0223440-mRNA-1
chr2_1 three_prime_UTR 73001628 73001998 73001628 ID=Msa0223440-mRNA-1:three_prime_utr;Parent=Msa0223440-mRNA-1
Gene Sequence

>Msa0223440

ATGGTTAACGCTAAGTTTCACCAGTACCAGGTTGTCGGAAGAGCACTTCCCACCGAAAAGGATGAACATCCTAAGATTTATCGCATGAAGCTTTGGGCAACCAACGAGGTTCGTGCCAAATCCAAGTTCTGGTATTTCTTGAGGAAGTTGAAGAAGGTTAAGAAAAGTAATGGACAAGTTTTGGCTATCAATGAGATCTTTGAGAAAAATCCTACCAAGATTAAGAACTATGGAATTTGGTTGCGTTATCAGAGTCGTACTGGTTATCACAATATGTACAAGGAATACCGTGATACTACTCTAAACGGTGCTGTGGAGACAATGTACAACGAAATGGCATCTCGTCATAGGGTCAGGTTTCCTTGCATCCAGATCATTAAGACCGCCACCATCCCAGCTAATCTGTGCAAAAGGGAGAGCACTAAGCAGTTCCACAATTCCAAAATCAAGTTCCCCTTGGTGTACAAGAAGATTAGGCCTCCTACTAGGAGCCTAAAAACAACCTACAAAGCTAAGAAGCCCAACCTGTTTATGTGA
Protein sequence

>Msa0223440

MVNAKFHQYQVVGRALPTEKDEHPKIYRMKLWATNEVRAKSKFWYFLRKLKKVKKSNGQVLAINEIFEKNPTKIKNYGIWLRYQSRTGYHNMYKEYRDTTLNGAVETMYNEMASRHRVRFPCIQIIKTATIPANLCKRESTKQFHNSKIKFPLVYKKIRPPTRSLKTTYKAKKPNLFM*