AlfalfaGEDB | Msa0223440

M. sativa cultivar zhongmu-4 / Msa0223440

KEGG: K02882
GO: GO:0002181 GO:0003674 GO:0003676 GO:0003723 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0015934 GO:0019538 GO:0022625 GO:0022626 GO:0032991 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0071704 GO:0097159 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF01775.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0223440	NP_001365661.1	100.000	178	0	0	1	178	1	178	3.25e-129	370

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0223440	sp\|Q9ATF5\|RL18A_CASSA	89.888	178	18	0	1	178	1	178	4.25e-119	337

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0223440	B7FJC3	100.000	178	0	0	1	178	1	178	1.55e-129	370

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0040220	Msa0223440	0.803530	3.336605e-49	-8.615850e-47
Msa0087910	Msa0223440	0.826491	2.635133e-54	-8.615850e-47
Msa0223440	Msa0264640	0.820398	7.006747e-53	-8.615850e-47
Msa0223440	Msa0301220	0.815561	8.678265e-52	-8.615850e-47
Msa0223440	Msa0369410	0.841593	4.325117e-58	-8.615850e-47
Msa0223440	Msa0463520	0.816245	6.107290e-52	-8.615850e-47
Msa0223440	Msa0596370	0.808852	2.526182e-50	-8.615850e-47
Msa0223440	Msa0597240	0.805585	1.243285e-49	-8.615850e-47
Msa0223440	Msa0694400	0.801278	9.710241e-49	-8.615850e-47
Msa0223440	Msa1040160	0.839638	1.405468e-57	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0223440	MtrunA17_Chr5g0442931	100.000	178	0	1	178	1	178	2.99e-133	370
Msa0223440	MtrunA17_Chr5g0442901	100.000	178	0	1	178	1	178	2.99e-133	370
Msa0223440	MtrunA17_Chr2g0328821	100.000	178	0	1	178	17	194	3.33e-133	370
Msa0223440	MtrunA17_Chr3g0095911	94.382	178	10	1	178	1	178	2.58e-125	350
Msa0223440	MtrunA17_Chr2g0286131	62.353	85	32	92	176	1	85	2.93e-34	117

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0223440	AT2G34480.1	90.230	174	17	5	178	5	178	3.01e-119	335
Msa0223440	AT2G34480.2	90.230	174	17	5	178	44	217	3.24e-119	336
Msa0223440	AT1G29965.1	87.079	178	23	1	178	1	178	1.24e-117	330
Msa0223440	AT3G14600.1	87.079	178	23	1	178	1	178	4.20e-117	329
Msa0223440	AT1G29970.2	89.017	173	19	6	178	140	312	1.08e-114	328

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Find 63 sgRNAs with CRISPR-Local

Find 242 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACTTACCAGAACTTGGATT+TGG	0.367421	2_1:+73003162	None:intergenic
AAGAAGGTTAAGAAAAGTAA+TGG	0.518066	2_1:-73003040	Msa0223440:CDS
AAGAGCACTTCCCACCGAAA+AGG	0.530461	2_1:-73003239	Msa0223440:CDS
AAGCAACTCTAACATGAGAA+GGG	0.603748	2_1:+73001959	None:intergenic
AATTCCATAGTTCTTAATCT+TGG	0.403701	2_1:+73002299	None:intergenic
ACAAAATACTTGATAAATAA+CGG	0.456124	2_1:+73001838	None:intergenic
ACAGAAAACTACAATCAGCT+CGG	0.620129	2_1:-73003394	Msa0223440:five_prime_UTR
ACGAAATGGCATCTCGTCAT+AGG	0.320455	2_1:-73002185	Msa0223440:CDS
ACTACAATCAGCTCGGAAAA+TGG	0.314844	2_1:-73003387	Msa0223440:exon
ACTGAATCACATAAACAGGT+TGG	0.563649	2_1:+73001993	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTTGTGTGTGAATCTTAACA+CGG	-	chr2_1:73001745-73001764	Msa0223440:three_prime_UTR	30.0%
ATTGTTGTAGGTATTTCTTG+AGG	-	chr2_1:73001983-73002002	Msa0223440:exon	30.0%
TCATAAACTGTATCATGGAA+TGG	+	chr2_1:73002242-73002261	None:intergenic	30.0%
ACAAGTCATAAACTGTATCA+TGG	+	chr2_1:73002247-73002266	None:intergenic	30.0%
AATTGTGGTGAAAGAATATG+TGG	-	chr2_1:73002615-73002634	Msa0223440:intron	30.0%
TAACAACTAAAGCAAACACA+GGG	+	chr2_1:73002651-73002670	None:intergenic	30.0%
ATAACAACTAAAGCAAACAC+AGG	+	chr2_1:73002652-73002671	None:intergenic	30.0%
TGGTTATCACAATATGTACA+AGG	-	chr2_1:73002799-73002818	Msa0223440:intron	30.0%
ATTCACTGAATCACATAAAC+AGG	+	chr2_1:73003066-73003085	None:intergenic	30.0%
TTTGTGTGTGAATCTTAACA+CGG	-	chr2_1:73001745-73001764	Msa0223440:three_prime_UTR	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	73001628	73003446	73001628	ID=Msa0223440;Name=Msa0223440
chr2_1	mRNA	73001628	73003446	73001628	ID=Msa0223440-mRNA-1;Parent=Msa0223440;Name=Msa0223440-mRNA-1;_AED=0.05;_eAED=0.05;_QI=56\|1\|1\|1\|1\|1\|4\|371\|178
chr2_1	exon	73001628	73002340	73001628	ID=Msa0223440-mRNA-1:exon:21283;Parent=Msa0223440-mRNA-1
chr2_1	exon	73003018	73003081	73003018	ID=Msa0223440-mRNA-1:exon:21282;Parent=Msa0223440-mRNA-1
chr2_1	exon	73003169	73003284	73003169	ID=Msa0223440-mRNA-1:exon:21281;Parent=Msa0223440-mRNA-1
chr2_1	exon	73003376	73003446	73003376	ID=Msa0223440-mRNA-1:exon:21280;Parent=Msa0223440-mRNA-1
chr2_1	five_prime_UTR	73003391	73003446	73003391	ID=Msa0223440-mRNA-1:five_prime_utr;Parent=Msa0223440-mRNA-1
chr2_1	CDS	73003376	73003390	73003376	ID=Msa0223440-mRNA-1:cds;Parent=Msa0223440-mRNA-1
chr2_1	CDS	73003169	73003284	73003169	ID=Msa0223440-mRNA-1:cds;Parent=Msa0223440-mRNA-1
chr2_1	CDS	73003018	73003081	73003018	ID=Msa0223440-mRNA-1:cds;Parent=Msa0223440-mRNA-1
chr2_1	CDS	73001999	73002340	73001999	ID=Msa0223440-mRNA-1:cds;Parent=Msa0223440-mRNA-1
chr2_1	three_prime_UTR	73001628	73001998	73001628	ID=Msa0223440-mRNA-1:three_prime_utr;Parent=Msa0223440-mRNA-1

Gene Sequence

>Msa0223440

ATGGTTAACGCTAAGTTTCACCAGTACCAGGTTGTCGGAAGAGCACTTCCCACCGAAAAGGATGAACATCCTAAGATTTATCGCATGAAGCTTTGGGCAACCAACGAGGTTCGTGCCAAATCCAAGTTCTGGTATTTCTTGAGGAAGTTGAAGAAGGTTAAGAAAAGTAATGGACAAGTTTTGGCTATCAATGAGATCTTTGAGAAAAATCCTACCAAGATTAAGAACTATGGAATTTGGTTGCGTTATCAGAGTCGTACTGGTTATCACAATATGTACAAGGAATACCGTGATACTACTCTAAACGGTGCTGTGGAGACAATGTACAACGAAATGGCATCTCGTCATAGGGTCAGGTTTCCTTGCATCCAGATCATTAAGACCGCCACCATCCCAGCTAATCTGTGCAAAAGGGAGAGCACTAAGCAGTTCCACAATTCCAAAATCAAGTTCCCCTTGGTGTACAAGAAGATTAGGCCTCCTACTAGGAGCCTAAAAACAACCTACAAAGCTAAGAAGCCCAACCTGTTTATGTGA

Protein sequence

>Msa0223440

MVNAKFHQYQVVGRALPTEKDEHPKIYRMKLWATNEVRAKSKFWYFLRKLKKVKKSNGQVLAINEIFEKNPTKIKNYGIWLRYQSRTGYHNMYKEYRDTTLNGAVETMYNEMASRHRVRFPCIQIIKTATIPANLCKRESTKQFHNSKIKFPLVYKKIRPPTRSLKTTYKAKKPNLFM*