AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0336360

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0336360 MtrunA17_Chr2g0322181 99.520 417 2 0 1 417 1 417 0.0 858
Msa0336360 MtrunA17_Chr4g0025401 91.169 419 32 3 1 417 1 416 0.0 686
Msa0336360 MtrunA17_Chr8g0334811 70.991 424 115 5 1 417 1 423 0.0 582
Msa0336360 MtrunA17_Chr7g0269841 37.322 351 180 9 14 358 27 343 2.18e-58 194
Msa0336360 MtrunA17_Chr4g0040501 33.333 360 204 10 14 358 5 343 7.01e-51 174
Msa0336360 MtrunA17_Chr2g0333221 32.143 364 221 11 11 357 68 422 2.86e-49 174
Msa0336360 MtrunA17_Chr4g0010781 34.463 354 186 11 14 347 87 414 1.25e-48 171
Msa0336360 MtrunA17_Chr3g0123861 33.144 353 217 9 14 357 83 425 2.45e-48 170
Msa0336360 MtrunA17_Chr2g0324921 32.234 394 154 9 14 358 5 334 2.35e-47 165
Msa0336360 MtrunA17_Chr2g0282741 33.333 360 199 11 14 358 5 338 3.74e-47 164
Msa0336360 MtrunA17_Chr3g0138471 32.213 357 194 7 14 358 5 325 4.37e-46 161
Msa0336360 MtrunA17_Chr6g0478651 31.793 368 225 11 14 365 69 426 5.87e-46 166
Msa0336360 MtrunA17_Chr2g0282761 34.018 341 199 8 14 343 5 330 1.61e-45 160
Msa0336360 MtrunA17_Chr4g0006391 30.151 398 161 9 14 358 5 338 7.86e-40 145
Msa0336360 MtrunA17_Chr3g0087741 30.645 372 212 15 1 355 80 422 4.40e-39 145
Msa0336360 MtrunA17_Chr5g0426951 27.957 372 221 11 5 355 75 420 4.14e-31 124
Msa0336360 MtrunA17_Chr5g0415751 36.552 145 90 1 214 358 18 160 2.65e-28 109
Msa0336360 MtrunA17_Chr3g0093111 34.752 141 90 1 218 358 172 310 1.34e-21 94.7
Msa0336360 MtrunA17_Chr3g0093111 27.136 199 108 5 14 180 3 196 6.77e-16 78.2
Msa0336360 MtrunA17_Chr3g0124801 34.483 145 92 2 215 358 149 291 4.08e-19 87.0
Msa0336360 MtrunA17_Chr2g0277721 32.639 144 96 1 215 358 117 259 4.80e-18 83.6
Msa0336360 MtrunA17_Chr8g0352451 46.392 97 43 3 12 104 71 162 4.31e-15 76.6
Msa0336360 MtrunA17_Chr7g0263971 52.564 78 31 2 4 76 360 436 3.01e-14 74.3
Msa0336360 MtrunA17_Chr5g0399201 43.750 80 38 1 10 82 9 88 3.68e-14 74.3
Msa0336360 MtrunA17_Chr1g0209551 52.941 68 28 1 15 78 18 85 8.18e-14 72.8
Msa0336360 MtrunA17_Chr1g0159511 43.878 98 44 4 12 104 5 96 2.14e-13 71.2
Msa0336360 MtrunA17_Chr1g0159491 48.571 70 32 2 13 78 6 75 5.92e-13 63.9
Msa0336360 MtrunA17_Chr5g0393371 40.206 97 49 3 12 104 5 96 1.94e-12 68.6
Msa0336360 MtrunA17_Chr5g0415731 32.215 149 63 5 11 146 3 126 3.20e-12 63.9
Msa0336360 MtrunA17_Chr5g0414471 37.255 102 50 3 15 105 27 125 1.14e-11 66.2
Msa0336360 MtrunA17_Chr7g0271891 40.000 75 37 1 14 80 17 91 3.61e-11 61.6
Msa0336360 MtrunA17_Chr3g0091361 47.297 74 34 2 14 82 6 79 4.37e-11 62.4
Msa0336360 MtrunA17_Chr7g0242951 46.154 65 32 1 15 76 29 93 7.81e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0336360 AT5G22060.1 83.055 419 69 2 1 417 1 419 0.0 684
Msa0336360 AT3G44110.1 85.036 421 58 3 1 417 1 420 0.0 655
Msa0336360 AT3G44110.2 87.651 332 39 2 1 331 1 331 0.0 530
Msa0336360 AT3G62600.1 35.775 355 182 11 14 358 27 345 7.75e-56 188
Msa0336360 AT1G80030.2 32.964 361 216 10 14 357 76 427 5.47e-53 184
Msa0336360 AT1G80030.3 32.964 361 216 10 14 357 76 427 5.47e-53 184
Msa0336360 AT1G80030.1 32.964 361 216 10 14 357 76 427 5.47e-53 184
Msa0336360 AT1G80030.4 32.964 361 216 10 14 357 76 427 6.91e-53 184
Msa0336360 AT2G20560.1 33.711 353 205 8 14 358 5 336 8.21e-50 172
Msa0336360 AT5G25530.2 32.597 362 198 9 14 358 5 337 9.30e-48 166
Msa0336360 AT5G48030.1 34.167 360 201 13 14 355 95 436 1.04e-47 169
Msa0336360 AT2G22360.1 32.075 371 229 10 14 378 87 440 5.79e-47 167
Msa0336360 AT4G39960.1 31.198 359 228 9 11 357 83 434 6.08e-45 162
Msa0336360 AT4G39960.2 31.198 359 228 9 11 357 83 434 6.08e-45 162
Msa0336360 AT5G25530.1 31.892 370 198 10 14 358 5 345 6.85e-45 159
Msa0336360 AT4G28480.1 31.198 359 217 8 14 358 5 347 1.28e-44 158
Msa0336360 AT3G08910.1 30.789 380 164 9 14 358 5 320 9.56e-40 145
Msa0336360 AT3G17830.1 30.303 363 225 10 12 355 62 415 5.42e-38 144
Msa0336360 AT5G01390.1 29.517 393 165 11 14 358 5 333 9.32e-38 140
Msa0336360 AT1G28210.3 27.895 380 229 11 7 368 42 394 1.08e-33 130
Msa0336360 AT1G28210.1 27.895 380 229 11 7 368 42 394 1.50e-33 130
Msa0336360 AT1G28210.2 27.895 380 229 11 7 368 42 394 1.84e-33 130
Msa0336360 AT2G20550.1 37.241 145 89 1 214 358 141 283 1.06e-26 108
Msa0336360 AT2G20550.2 37.241 145 89 1 214 358 141 283 1.06e-26 108
Msa0336360 AT3G47940.1 36.552 145 90 1 214 358 204 346 1.82e-26 109
Msa0336360 AT3G47940.1 34.021 194 67 4 14 146 5 198 1.12e-20 93.2
Msa0336360 AT5G01390.2 35.507 138 87 1 221 358 140 275 1.11e-23 100
Msa0336360 AT5G01390.2 40.741 108 43 3 14 100 5 112 1.78e-16 79.7
Msa0336360 AT4G28480.2 34.694 147 92 2 212 358 147 289 1.68e-23 100
Msa0336360 AT4G28480.2 43.750 112 50 2 14 120 5 108 6.04e-18 84.3
Msa0336360 AT1G59725.1 36.957 138 85 1 221 358 193 328 7.45e-23 99.0
Msa0336360 AT1G59725.1 32.955 176 75 4 14 146 5 180 4.32e-19 88.2
Msa0336360 AT1G10350.1 36.552 145 87 3 214 358 207 346 7.94e-23 99.4
Msa0336360 AT1G10350.1 32.308 195 70 6 14 146 5 199 3.69e-19 88.6
Msa0336360 AT5G01390.4 35.870 184 70 6 14 149 5 188 7.49e-22 94.0
Msa0336360 AT1G11040.1 37.500 136 83 1 215 350 291 424 4.04e-20 92.4
Msa0336360 AT1G44160.1 32.593 135 89 1 224 358 221 353 1.71e-17 84.0
Msa0336360 AT5G01390.3 40.741 108 43 3 14 100 5 112 9.72e-17 79.7
Msa0336360 AT2G21510.5 44.330 97 45 3 12 104 5 96 1.79e-15 77.4
Msa0336360 AT2G21510.3 44.330 97 45 3 12 104 5 96 2.50e-15 77.4
Msa0336360 AT2G21510.1 44.330 97 45 3 12 104 5 96 2.50e-15 77.4
Msa0336360 AT2G21510.2 44.330 97 45 3 12 104 5 96 2.57e-15 77.4
Msa0336360 AT2G21510.4 44.330 97 45 3 12 104 5 96 2.57e-15 77.4
Msa0336360 AT5G05750.1 41.748 103 46 3 14 102 115 217 4.26e-14 72.8
Msa0336360 AT4G39150.3 42.268 97 47 3 12 104 5 96 9.46e-14 72.4
Msa0336360 AT4G39150.1 42.268 97 47 3 12 104 5 96 9.46e-14 72.4
Msa0336360 AT4G39150.2 42.268 97 47 3 12 104 5 96 9.46e-14 72.4
Msa0336360 AT1G59980.2 47.059 68 32 1 15 78 25 92 4.24e-13 70.5
Msa0336360 AT1G59980.1 47.059 68 32 1 15 78 25 92 6.38e-13 70.5
Msa0336360 AT5G03160.1 50.000 94 40 4 4 92 361 452 1.18e-12 69.7
Msa0336360 AT1G68370.1 51.471 68 29 1 15 78 19 86 1.25e-12 69.7
Msa0336360 AT3G57340.1 42.424 99 42 3 14 97 114 212 1.45e-12 68.9
Msa0336360 AT3G57340.2 42.424 99 42 3 14 97 114 212 1.45e-12 68.9
Msa0336360 AT3G08970.1 47.297 74 36 1 15 85 29 102 1.74e-12 69.7
Msa0336360 AT3G08970.2 47.297 74 36 1 15 85 29 102 1.74e-12 69.7
Msa0336360 AT1G24120.1 37.255 102 50 4 15 105 22 120 3.95e-12 68.2
Msa0336360 AT3G12170.2 46.479 71 34 1 8 74 42 112 1.25e-11 65.5
Msa0336360 AT3G12170.1 31.707 164 84 6 8 166 6 146 1.26e-11 65.1
Msa0336360 AT1G21080.3 39.175 97 49 3 12 104 5 95 2.07e-11 65.9
Msa0336360 AT1G21080.1 39.175 97 49 3 12 104 5 95 2.19e-11 65.5
Msa0336360 AT2G35720.1 38.824 85 45 1 5 82 7 91 2.76e-11 65.9
Msa0336360 AT1G74250.1 44.928 69 29 2 14 73 12 80 3.79e-11 65.5
Msa0336360 AT5G49060.2 46.269 67 33 1 11 74 97 163 4.00e-11 64.7
Msa0336360 AT5G49060.1 46.269 67 33 1 11 74 97 163 4.00e-11 64.7
Msa0336360 AT1G76700.1 39.394 99 51 3 12 106 5 98 6.20e-11 64.3
Msa0336360 AT4G10130.2 43.939 66 30 1 14 72 12 77 6.22e-11 61.6
Msa0336360 AT4G10130.4 43.939 66 30 1 14 72 12 77 6.22e-11 61.6
Msa0336360 AT4G10130.1 43.939 66 30 1 14 72 12 77 6.22e-11 61.6
Msa0336360 AT4G10130.3 43.939 66 30 1 14 72 12 77 6.22e-11 61.6
Msa0336360 AT4G10130.5 43.939 66 30 1 14 72 12 77 6.22e-11 61.6
Msa0336360 AT1G56300.1 41.176 68 32 1 12 71 12 79 7.86e-11 60.8
Msa0336360 AT5G06910.1 45.714 70 34 1 9 74 25 94 9.22e-11 62.8

Find 137 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCACAAAGCTTGAAAATT+AGG 0.093537 2_4:+55741213 Msa0336360:intron
TTCGGTGGAGGTGGATTTCC+TGG 0.267655 2_4:+55742174 Msa0336360:CDS
TTCACAAAGCTTGAAAATTA+GGG 0.287664 2_4:+55741214 Msa0336360:intron
TCACCCCTTGAAGGTCTCTT+TGG 0.287702 2_4:+55742346 Msa0336360:CDS
CCAATTTGCGCTGACTCATT+TGG 0.293928 2_4:+55743015 Msa0336360:CDS
GGAATGGGTGGTGGCGGTGG+TGG 0.294243 2_4:+55742120 Msa0336360:CDS
TCAGGATCACCACCCTTATC+AGG 0.295880 2_4:-55741402 None:intergenic
CCGTATAAAGATATCCTATA+TGG 0.308033 2_4:+55743614 Msa0336360:three_prime_UTR
CCAAATGAGTCAGCGCAAAT+TGG 0.320936 2_4:-55743015 None:intergenic
TTCACCCTTTCGTTTGAACT+TGG 0.337178 2_4:-55742942 None:intergenic
GAAGATATCTATGAGGCATT+TGG 0.338997 2_4:+55742584 Msa0336360:CDS
GAAGGAATGGGTGGTGGCGG+TGG 0.340853 2_4:+55742117 Msa0336360:CDS
ACTAAATACTATGAGATTCT+TGG 0.343941 2_4:+55741312 Msa0336360:CDS
AACTGAGATACAGGCCTTGC+TGG 0.344090 2_4:-55743335 None:intergenic
CCCCTTGAAGGTCTCTTTGG+AGG 0.348117 2_4:+55742349 Msa0336360:CDS
TTGCAGCCGGACACAGTTAC+TGG 0.350028 2_4:+55742884 Msa0336360:intron
CACCACCCATTCCTTCCTTA+AGG 0.353366 2_4:-55742110 None:intergenic
CCATATAGGATATCTTTATA+CGG 0.354474 2_4:-55743614 None:intergenic
TTAGTGTTATCACTCTTCTT+AGG 0.362823 2_4:-55741294 None:intergenic
TGTGCTGGTTGTCAAGGTTC+TGG 0.368082 2_4:+55742558 Msa0336360:CDS
TACGCTTTCTTCAAATCATC+AGG 0.375393 2_4:-55741354 None:intergenic
TCTTGATCAGGTCAAGGCTT+TGG 0.379949 2_4:+55743301 Msa0336360:CDS
TTGCATTTGCTGAATCATAT+TGG 0.385235 2_4:-55742610 None:intergenic
AAAGCTTGAAAATTAGGGTT+AGG 0.389474 2_4:+55741219 Msa0336360:intron
ACCACCCATTCCTTCCTTAA+GGG 0.391516 2_4:-55742109 None:intergenic
CACCCTTTCGTTTGAACTTG+GGG 0.398325 2_4:-55742940 None:intergenic
TCACCCTTTCGTTTGAACTT+GGG 0.404206 2_4:-55742941 None:intergenic
AAGATATCTATGAGGCATTT+GGG 0.411623 2_4:+55742585 Msa0336360:CDS
ATGAAAGGCCTCTGGTACAT+TGG 0.411721 2_4:-55743227 None:intergenic
TTGTGAAATTTGAAGATGTT+TGG 0.412460 2_4:+55741264 Msa0336360:exon
TGAGTCTCTGTCTCTTGATC+AGG 0.415051 2_4:+55743289 Msa0336360:CDS
GTGCTCAGCAGTAATGAGTT+TGG 0.416716 2_4:+55743501 Msa0336360:exon
AATTATAAAATTAACCATAT+AGG 0.417706 2_4:-55743628 None:intergenic
ACATCCTTGCAATGAATGTA+AGG 0.418761 2_4:+55742632 Msa0336360:CDS
CTTTGGAGGATCTTTACCTT+GGG 0.422824 2_4:+55742363 Msa0336360:CDS
AGAGCTTGCACAAGCTTATG+AGG 0.426543 2_4:+55742035 Msa0336360:CDS
TATTATCCTATGATTTAGAT+AGG 0.429059 2_4:+55743736 Msa0336360:three_prime_UTR
ATGTCTGTCAACTGAGATAC+AGG 0.432545 2_4:-55743344 None:intergenic
AAAGATGAAAAGATGTCAAA+TGG 0.433471 2_4:-55742150 None:intergenic
CCTTCTCTCCCTTGCACTGT+GGG 0.438316 2_4:-55742689 None:intergenic
TCATATACCTCACGCTTCTC+AGG 0.445588 2_4:-55742069 None:intergenic
GAGTTTGGAGATGTCCTTCA+TGG 0.448651 2_4:+55743516 Msa0336360:three_prime_UTR
TCCCTCACTGAGGCTTTATG+TGG 0.451630 2_4:+55742989 Msa0336360:CDS
TGCAATGAATGTAAGGGTAC+TGG 0.456888 2_4:+55742639 Msa0336360:CDS
TCATCTTTCTTCGGTGGAGG+TGG 0.461355 2_4:+55742165 Msa0336360:CDS
GTATGGTGAAGATGCCCTTA+AGG 0.462316 2_4:+55742095 Msa0336360:CDS
GAGATAGTGAATGATTATCT+TGG 0.463868 2_4:+55743847 Msa0336360:three_prime_UTR
GGTTTGATTTAATCAATAGT+TGG 0.465413 2_4:-55743045 None:intergenic
CGTGAGGTATATGATCAGTA+TGG 0.471347 2_4:+55742078 Msa0336360:CDS
TCTTTGGAGGATCTTTACCT+TGG 0.472410 2_4:+55742362 Msa0336360:CDS
GATTAAGAATCATCCTGATA+AGG 0.476346 2_4:+55741389 Msa0336360:CDS
GGATGTCGTTCACCCCTTGA+AGG 0.478745 2_4:+55742337 Msa0336360:CDS
AGATCGATGCCCACAGTGCA+AGG 0.481792 2_4:+55742680 Msa0336360:CDS
ATTAAGAATCATCCTGATAA+GGG 0.485570 2_4:+55741390 Msa0336360:CDS
TACAGGAAAGGATCAAAGTC+TGG 0.495336 2_4:+55742522 Msa0336360:intron
ACTAACCTAGGGGAAATAAT+CGG 0.496006 2_4:-55743786 None:intergenic
TTGAAGAAGAGACGAGAAGA+AGG 0.496972 2_4:+55743411 Msa0336360:CDS
TGCCCTTCATGAAAGGCCTC+TGG 0.498481 2_4:-55743235 None:intergenic
CCAGGCTTGACAACTTCTCC+AGG 0.499453 2_4:-55743067 None:intergenic
ATATCTCCAGTAACTGTGTC+CGG 0.501009 2_4:-55742890 None:intergenic
GGTGCTTCGATGACCTGTGC+TGG 0.505989 2_4:+55742543 Msa0336360:CDS
CTTGAAGTTCATGTTGAGAA+GGG 0.510818 2_4:+55742732 Msa0336360:CDS
ATCCTCCAAAGAGACCTTCA+AGG 0.518323 2_4:-55742351 None:intergenic
AAGGAAGGAATGGGTGGTGG+CGG 0.520461 2_4:+55742114 Msa0336360:CDS
ATAATGAATTACACTAACCT+AGG 0.520955 2_4:-55743798 None:intergenic
TACAAGGCAATCAATGATGA+AGG 0.521364 2_4:+55743200 Msa0336360:CDS
TTTGTACCTATCTAAATCAT+AGG 0.526396 2_4:-55743742 None:intergenic
CACCCCAAGTTCAAACGAAA+GGG 0.528668 2_4:+55742938 Msa0336360:CDS
AGATGCCCTTAAGGAAGGAA+TGG 0.532671 2_4:+55742104 Msa0336360:CDS
TGATCAAGAGACAGAGACTC+AGG 0.533617 2_4:-55743287 None:intergenic
CCTGGAGAAGTTGTCAAGCC+TGG 0.534032 2_4:+55743067 Msa0336360:CDS
GGTCAGAAGATTACCTTCCC+TGG 0.534089 2_4:+55742765 Msa0336360:CDS
TTCATCAGCTTCACCAGGGA+AGG 0.534704 2_4:-55742778 None:intergenic
TAACACATACCCCTTGCACT+TGG 0.540192 2_4:-55742426 None:intergenic
ACTTGAAGTTCATGTTGAGA+AGG 0.543623 2_4:+55742731 Msa0336360:CDS
TAATGAATTACACTAACCTA+GGG 0.544568 2_4:-55743797 None:intergenic
GATAATAGAGCATGCAAGGA+AGG 0.547276 2_4:-55743720 None:intergenic
CTTAAGGAAGGAATGGGTGG+TGG 0.547958 2_4:+55742111 Msa0336360:CDS
TGTCTTGTGCTCCAAGTGCA+AGG 0.549478 2_4:+55742415 Msa0336360:CDS
TACCAGAGGCCTTTCATGAA+GGG 0.550239 2_4:+55743233 Msa0336360:CDS
ACCGCTTCATCAGCTTCACC+AGG 0.550702 2_4:-55742783 None:intergenic
TCTGTCTCTTGATCAGGTCA+AGG 0.553837 2_4:+55743295 Msa0336360:CDS
GATGCCCTTAAGGAAGGAAT+GGG 0.556456 2_4:+55742105 Msa0336360:CDS
CCCACAGTGCAAGGGAGAGA+AGG 0.557002 2_4:+55742689 Msa0336360:CDS
CGTCTTTGTACTTCAACAGA+AGG 0.558455 2_4:+55742913 Msa0336360:CDS
TAAAGCTTGCCCTTCATGAA+AGG 0.559348 2_4:-55743242 None:intergenic
GTCTTGTGCTCCAAGTGCAA+GGG 0.560067 2_4:+55742416 Msa0336360:CDS
ATGACCTGTGCTGGTTGTCA+AGG 0.561075 2_4:+55742552 Msa0336360:CDS
GTTGAGAAGGGGATGCAGAA+TGG 0.563375 2_4:+55742744 Msa0336360:CDS
TGGTTACGCAGATTCATACA+AGG 0.564992 2_4:+55743184 Msa0336360:intron
GTACCAGAGGCCTTTCATGA+AGG 0.566111 2_4:+55743232 Msa0336360:CDS
ACTGAGATACAGGCCTTGCT+GGG 0.567469 2_4:-55743334 None:intergenic
GGTGAAGATGCCCTTAAGGA+AGG 0.569050 2_4:+55742099 Msa0336360:CDS
TCAGTTGACAGACATGGAGC+TGG 0.570239 2_4:+55743352 Msa0336360:CDS
GTGGTAGTAGCAGAGGAAGA+AGG 0.572018 2_4:+55742300 Msa0336360:CDS
ACAGGTGGTGGTAGTAGCAG+AGG 0.575959 2_4:+55742293 Msa0336360:intron
ATAGGATAATAGAGCATGCA+AGG 0.576670 2_4:-55743724 None:intergenic
AGAACCTTGACAACCAGCAC+AGG 0.585983 2_4:-55742556 None:intergenic
TGGAGACAATTCTCCCAGCA+AGG 0.587524 2_4:+55743321 Msa0336360:CDS
CCCTGGTGAAGCTGATGAAG+CGG 0.588726 2_4:+55742782 Msa0336360:CDS
ACACCCCAAGTTCAAACGAA+AGG 0.592574 2_4:+55742937 Msa0336360:CDS
AAGCTAAAACACATCCATGA+AGG 0.592582 2_4:-55743530 None:intergenic
TGTACTCTTTGAGCACCACC+AGG 0.594168 2_4:-55743476 None:intergenic
TCCTCCAAAGAGACCTTCAA+GGG 0.596017 2_4:-55742350 None:intergenic
TTGAAGTTCATGTTGAGAAG+GGG 0.603095 2_4:+55742733 Msa0336360:CDS
GTACCCTTACATTCATTGCA+AGG 0.605875 2_4:-55742636 None:intergenic
AGCTTTGTTGGGCAAAACAG+TGG 0.607380 2_4:+55743548 Msa0336360:three_prime_UTR
TTTCACCGATTATTTCCCCT+AGG 0.611137 2_4:+55743781 Msa0336360:three_prime_UTR
CAGGCTTGACAACTTCTCCA+GGG 0.612975 2_4:-55743066 None:intergenic
TAAGGGTGGTGATCCTGAAA+AGG 0.613088 2_4:+55741407 Msa0336360:CDS
TGTATCTCAGTTGACAGACA+TGG 0.616220 2_4:+55743346 Msa0336360:CDS
GCCCTTAAGGAAGGAATGGG+TGG 0.620209 2_4:+55742108 Msa0336360:CDS
GATGAGGATGATGAAATGCC+TGG 0.624859 2_4:+55743458 Msa0336360:CDS
AAGGCAGCAAGCTCAGCAAG+AGG 0.624868 2_4:+55743430 Msa0336360:CDS
ACCTTCTCTCCCTTGCACTG+TGG 0.630501 2_4:-55742690 None:intergenic
GATCGATGCCCACAGTGCAA+GGG 0.633588 2_4:+55742681 Msa0336360:CDS
AAAGCTTCTTAGATGTCCCA+AGG 0.634241 2_4:-55742379 None:intergenic
TCTTGTGCTCCAAGTGCAAG+GGG 0.635966 2_4:+55742417 Msa0336360:CDS
CTATTGATTAAATCAAACCC+TGG 0.637960 2_4:+55743049 Msa0336360:CDS
AGAGGAAGAAGGCAGAGACG+TGG 0.638556 2_4:+55742311 Msa0336360:CDS
CATCCTTGCAATGAATGTAA+GGG 0.643345 2_4:+55742633 Msa0336360:CDS
AAGAAGGCAGAGACGTGGTG+AGG 0.643979 2_4:+55742316 Msa0336360:CDS
TCAGCAAGAGGCATATGATG+AGG 0.654664 2_4:+55743442 Msa0336360:CDS
ACCACATAAAGCCTCAGTGA+GGG 0.659830 2_4:-55742990 None:intergenic
CATGGAGCTGGATGAGTGTG+AGG 0.667513 2_4:+55743364 Msa0336360:CDS
GATCAAGAGACAGAGACTCA+GGG 0.667543 2_4:-55743286 None:intergenic
AAGGAATGCCAATGTACCAG+AGG 0.667557 2_4:+55743219 Msa0336360:CDS
CTGGTATGAAGATATCTATG+AGG 0.673684 2_4:+55742577 Msa0336360:CDS
GAGTGATCCTGAGAAGCGTG+AGG 0.676105 2_4:+55742062 Msa0336360:CDS
AAGAATCATCCTGATAAGGG+TGG 0.677133 2_4:+55741393 Msa0336360:CDS
CCGCTTCATCAGCTTCACCA+GGG 0.689740 2_4:-55742782 None:intergenic
GCTTTACATTCACTTCACAG+TGG 0.719754 2_4:+55743259 Msa0336360:CDS
CCTCCAAAGAGACCTTCAAG+GGG 0.727234 2_4:-55742349 None:intergenic
AACCACATAAAGCCTCAGTG+AGG 0.740190 2_4:-55742991 None:intergenic
AATGAATTACACTAACCTAG+GGG 0.742373 2_4:-55743796 None:intergenic
GCACACATTGTCCCTCACTG+AGG 0.763892 2_4:+55742979 Msa0336360:CDS
GAGGATGATGAAATGCCTGG+TGG 0.804018 2_4:+55743461 Msa0336360:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTGAGATTTTTAAATTA+AGG + chr2_4:55741602-55741621 Msa0336360:intron 10.0%
!!! ATTTGAGATTTTTAAATTAA+GGG + chr2_4:55741603-55741622 Msa0336360:intron 10.0%
!! AATTATAAAATTAACCATAT+AGG - chr2_4:55743631-55743650 None:intergenic 10.0%
!! TTATCTTTGAATTTGATTAT+TGG + chr2_4:55741440-55741459 Msa0336360:intron 15.0%
!!! TTTGAGATTTTTAAATTAAG+GGG + chr2_4:55741604-55741623 Msa0336360:intron 15.0%
!!! AAAAGTGATTTTTTTACTAT+AGG + chr2_4:55741644-55741663 Msa0336360:intron 15.0%
!! ATTGAATTATATAATTGTTG+TGG + chr2_4:55741804-55741823 Msa0336360:intron 15.0%
!! ATTAGTTAATTGATGAAATT+AGG + chr2_4:55741975-55741994 Msa0336360:intron 15.0%
!! TTAGTTAATTGATGAAATTA+GGG + chr2_4:55741976-55741995 Msa0336360:intron 15.0%
!!! CTTGTTTGTTATTTTAATTT+AGG + chr2_4:55742205-55742224 Msa0336360:intron 15.0%
!!! TTATTTTAATTTAGGAGATT+AGG + chr2_4:55742213-55742232 Msa0336360:intron 15.0%
!!! TATTTTAATTTAGGAGATTA+GGG + chr2_4:55742214-55742233 Msa0336360:intron 15.0%
!!! AAAATTTTAAAGATACAATC+AGG + chr2_4:55743825-55743844 Msa0336360:three_prime_UTR 15.0%
!!! TAAAAAAAAACAACCTTTTC+AGG - chr2_4:55741423-55741442 None:intergenic 20.0%
!! TAGTGATTAGAATTGAATTT+AGG + chr2_4:55741504-55741523 Msa0336360:intron 20.0%
!! AGTGATTAGAATTGAATTTA+GGG + chr2_4:55741505-55741524 Msa0336360:intron 20.0%
!!! ATTGTTAGTTGTATTGATAT+TGG + chr2_4:55741850-55741869 Msa0336360:intron 20.0%
!!! TTGTTAGTTGTATTGATATT+GGG + chr2_4:55741851-55741870 Msa0336360:intron 20.0%
!!! AATTGTGATTAGTTAGTTTT+AGG + chr2_4:55741895-55741914 Msa0336360:intron 20.0%
!!! ATTGTGATTAGTTAGTTTTA+GGG + chr2_4:55741896-55741915 Msa0336360:intron 20.0%
!! AAAAATTGCTTAATAAGAGA+GGG - chr2_4:55743699-55743718 None:intergenic 20.0%
!! AAAAAATTGCTTAATAAGAG+AGG - chr2_4:55743700-55743719 None:intergenic 20.0%
!! TATTATCCTATGATTTAGAT+AGG + chr2_4:55743736-55743755 Msa0336360:three_prime_UTR 20.0%
!! ATTCTTTTGTTAGATCTCAA+CGG - chr2_4:55741192-55741211 None:intergenic 25.0%
! ATTCACAAAGCTTGAAAATT+AGG + chr2_4:55741213-55741232 Msa0336360:intron 25.0%
! TTCACAAAGCTTGAAAATTA+GGG + chr2_4:55741214-55741233 Msa0336360:intron 25.0%
! TTGTGAAATTTGAAGATGTT+TGG + chr2_4:55741264-55741283 Msa0336360:exon 25.0%
! ACTAAATACTATGAGATTCT+TGG + chr2_4:55741312-55741331 Msa0336360:CDS 25.0%
! ATTAAGAATCATCCTGATAA+GGG + chr2_4:55741390-55741409 Msa0336360:CDS 25.0%
! TATTGGATTATCTGATTGAT+TGG + chr2_4:55741457-55741476 Msa0336360:intron 25.0%
!! ATTGGATTTGATAATTGTTG+TGG + chr2_4:55741475-55741494 Msa0336360:intron 25.0%
! TTGGTAGAATGATATTTGTT+AGG + chr2_4:55741558-55741577 Msa0336360:intron 25.0%
!! TGGTAGAATGATATTTGTTA+GGG + chr2_4:55741559-55741578 Msa0336360:intron 25.0%
!!! TTGGTATTGATTATGCAATT+AGG + chr2_4:55741726-55741745 Msa0336360:intron 25.0%
! ATTGATTATGCAATTAGGAT+TGG + chr2_4:55741731-55741750 Msa0336360:intron 25.0%
! ATTGGGTTATATAATTGTTG+TGG + chr2_4:55741868-55741887 Msa0336360:intron 25.0%
! AAAGATGAAAAGATGTCAAA+TGG - chr2_4:55742153-55742172 None:intergenic 25.0%
! ATCATATTGTATTTGTGAAC+AGG + chr2_4:55742275-55742294 Msa0336360:intron 25.0%
!!! GTTTGTTGGTATTTTTCTAA+TGG + chr2_4:55742467-55742486 Msa0336360:intron 25.0%
!! GGTTTGATTTAATCAATAGT+TGG - chr2_4:55743048-55743067 None:intergenic 25.0%
!! TGCTGAAATTTTCAATCTTT+TGG + chr2_4:55743164-55743183 Msa0336360:intron 25.0%
! CCATATAGGATATCTTTATA+CGG - chr2_4:55743617-55743636 None:intergenic 25.0%
! TTTGTACCTATCTAAATCAT+AGG - chr2_4:55743745-55743764 None:intergenic 25.0%
! TAATGAATTACACTAACCTA+GGG - chr2_4:55743800-55743819 None:intergenic 25.0%
! ATAATGAATTACACTAACCT+AGG - chr2_4:55743801-55743820 None:intergenic 25.0%
AAAGCTTGAAAATTAGGGTT+AGG + chr2_4:55741219-55741238 Msa0336360:intron 30.0%
TTAGTGTTATCACTCTTCTT+AGG - chr2_4:55741297-55741316 None:intergenic 30.0%
GATTAAGAATCATCCTGATA+AGG + chr2_4:55741389-55741408 Msa0336360:CDS 30.0%
!! AGGGTTAGTTTGATTGAATT+AGG + chr2_4:55741524-55741543 Msa0336360:intron 30.0%
!! GGGTTAGTTTGATTGAATTA+GGG + chr2_4:55741525-55741544 Msa0336360:intron 30.0%
TTTGATTGAATTAGGGTTGT+GGG + chr2_4:55741532-55741551 Msa0336360:intron 30.0%
TTGTTAGGGTTTATTTGTCA+TGG + chr2_4:55741573-55741592 Msa0336360:intron 30.0%
AATATCAATCCCATTAGCTA+CGG - chr2_4:55741674-55741693 None:intergenic 30.0%
! TTACCCAATTGATTGTTGTT+AGG + chr2_4:55741699-55741718 Msa0336360:intron 30.0%
ACACCTAACAACAATCAATT+GGG - chr2_4:55741705-55741724 None:intergenic 30.0%
TACACCTAACAACAATCAAT+TGG - chr2_4:55741706-55741725 None:intergenic 30.0%
! TTGATTGTTGTTAGGTGTAT+TGG + chr2_4:55741707-55741726 Msa0336360:intron 30.0%
! GACATCTTTTCATCTTTCTT+CGG + chr2_4:55742156-55742175 Msa0336360:CDS 30.0%
ATATTGTATTTGTGAACAGG+TGG + chr2_4:55742278-55742297 Msa0336360:intron 30.0%
! TTGGTATTTTTCTAATGGCT+CGG + chr2_4:55742472-55742491 Msa0336360:intron 30.0%
!! TTGTTGATTGGTTTGATTAC+AGG + chr2_4:55742505-55742524 Msa0336360:intron 30.0%
! AAGATATCTATGAGGCATTT+GGG + chr2_4:55742585-55742604 Msa0336360:CDS 30.0%
! TTGCATTTGCTGAATCATAT+TGG - chr2_4:55742613-55742632 None:intergenic 30.0%
! CTATTGATTAAATCAAACCC+TGG + chr2_4:55743049-55743068 Msa0336360:CDS 30.0%
ATGTGAATAATCTACATACC+AGG - chr2_4:55743088-55743107 None:intergenic 30.0%
GTATGTAGATTATTCACATC+TGG + chr2_4:55743089-55743108 Msa0336360:intron 30.0%
CCGTATAAAGATATCCTATA+TGG + chr2_4:55743614-55743633 Msa0336360:three_prime_UTR 30.0%
AATGAATTACACTAACCTAG+GGG - chr2_4:55743799-55743818 None:intergenic 30.0%
TACGCTTTCTTCAAATCATC+AGG - chr2_4:55741357-55741376 None:intergenic 35.0%
GTTTGATTGAATTAGGGTTG+TGG + chr2_4:55741531-55741550 Msa0336360:intron 35.0%
ATATCAATCCCATTAGCTAC+GGG - chr2_4:55741673-55741692 None:intergenic 35.0%
AGGGTAGTGTGATTGAATTA+AGG + chr2_4:55741915-55741934 Msa0336360:intron 35.0%
AAATCAAACTACCATAACCC+AGG - chr2_4:55741941-55741960 None:intergenic 35.0%
!! GGGTTATGGTAGTTTGATTT+TGG + chr2_4:55741941-55741960 Msa0336360:intron 35.0%
! ATCTTTTCATCTTTCTTCGG+TGG + chr2_4:55742159-55742178 Msa0336360:CDS 35.0%
AACAAACAAGAAACCATACC+AGG - chr2_4:55742195-55742214 None:intergenic 35.0%
GGAGTTCTTACATTGTTGAT+TGG + chr2_4:55742493-55742512 Msa0336360:intron 35.0%
!! GATTGGTTTGATTACAGGAA+AGG + chr2_4:55742510-55742529 Msa0336360:intron 35.0%
CTGGTATGAAGATATCTATG+AGG + chr2_4:55742577-55742596 Msa0336360:CDS 35.0%
! GAAGATATCTATGAGGCATT+TGG + chr2_4:55742584-55742603 Msa0336360:CDS 35.0%
ACATCCTTGCAATGAATGTA+AGG + chr2_4:55742632-55742651 Msa0336360:CDS 35.0%
CATCCTTGCAATGAATGTAA+GGG + chr2_4:55742633-55742652 Msa0336360:CDS 35.0%
ACTTGAAGTTCATGTTGAGA+AGG + chr2_4:55742731-55742750 Msa0336360:CDS 35.0%
CTTGAAGTTCATGTTGAGAA+GGG + chr2_4:55742732-55742751 Msa0336360:CDS 35.0%
TTGAAGTTCATGTTGAGAAG+GGG + chr2_4:55742733-55742752 Msa0336360:CDS 35.0%
! GACTGAATTTTGTTCGTCTT+TGG + chr2_4:55743141-55743160 Msa0336360:intron 35.0%
TACAAGGCAATCAATGATGA+AGG + chr2_4:55743200-55743219 Msa0336360:CDS 35.0%
AAGCTAAAACACATCCATGA+AGG - chr2_4:55743533-55743552 None:intergenic 35.0%
!!! TGGATGTGTTTTAGCTTTGT+TGG + chr2_4:55743536-55743555 Msa0336360:three_prime_UTR 35.0%
!!! GGATGTGTTTTAGCTTTGTT+GGG + chr2_4:55743537-55743556 Msa0336360:three_prime_UTR 35.0%
! CTGCAATTTTGCAAACACAA+GGG - chr2_4:55743658-55743677 None:intergenic 35.0%
ATAGGATAATAGAGCATGCA+AGG - chr2_4:55743727-55743746 None:intergenic 35.0%
ACTAACCTAGGGGAAATAAT+CGG - chr2_4:55743789-55743808 None:intergenic 35.0%
AAGAATCATCCTGATAAGGG+TGG + chr2_4:55741393-55741412 Msa0336360:CDS 40.0%
TATCAATCCCATTAGCTACG+GGG - chr2_4:55741672-55741691 None:intergenic 40.0%
AGTGTGATTGAATTAAGGCC+TGG + chr2_4:55741920-55741939 Msa0336360:intron 40.0%
GTGTGATTGAATTAAGGCCT+GGG + chr2_4:55741921-55741940 Msa0336360:intron 40.0%
TTGAATTAAGGCCTGGGTTA+TGG + chr2_4:55741927-55741946 Msa0336360:intron 40.0%
CGTGAGGTATATGATCAGTA+TGG + chr2_4:55742078-55742097 Msa0336360:CDS 40.0%
! TTTTCATCTTTCTTCGGTGG+AGG + chr2_4:55742162-55742181 Msa0336360:CDS 40.0%
TTGTATTTGTGAACAGGTGG+TGG + chr2_4:55742281-55742300 Msa0336360:intron 40.0%
! TCTTTGGAGGATCTTTACCT+TGG + chr2_4:55742362-55742381 Msa0336360:CDS 40.0%
! CTTTGGAGGATCTTTACCTT+GGG + chr2_4:55742363-55742382 Msa0336360:CDS 40.0%
AAAGCTTCTTAGATGTCCCA+AGG - chr2_4:55742382-55742401 None:intergenic 40.0%
AGGTCTATCTGCATGTTTGT+TGG + chr2_4:55742453-55742472 Msa0336360:intron 40.0%
TACAGGAAAGGATCAAAGTC+TGG + chr2_4:55742522-55742541 Msa0336360:intron 40.0%
GTACCCTTACATTCATTGCA+AGG - chr2_4:55742639-55742658 None:intergenic 40.0%
! TGCAATGAATGTAAGGGTAC+TGG + chr2_4:55742639-55742658 Msa0336360:CDS 40.0%
! TACAGTTCCTTAGTTTGAGC+TGG + chr2_4:55742819-55742838 Msa0336360:intron 40.0%
CAACAATCCAGCTCAAACTA+AGG - chr2_4:55742829-55742848 None:intergenic 40.0%
CTTATTGTTTGTCTTGCAGC+CGG + chr2_4:55742871-55742890 Msa0336360:intron 40.0%
ATATCTCCAGTAACTGTGTC+CGG - chr2_4:55742893-55742912 None:intergenic 40.0%
CGTCTTTGTACTTCAACAGA+AGG + chr2_4:55742913-55742932 Msa0336360:CDS 40.0%
TCACCCTTTCGTTTGAACTT+GGG - chr2_4:55742944-55742963 None:intergenic 40.0%
TTCACCCTTTCGTTTGAACT+TGG - chr2_4:55742945-55742964 None:intergenic 40.0%
TGGTTACGCAGATTCATACA+AGG + chr2_4:55743184-55743203 Msa0336360:intron 40.0%
TAAAGCTTGCCCTTCATGAA+AGG - chr2_4:55743245-55743264 None:intergenic 40.0%
GCTTTACATTCACTTCACAG+TGG + chr2_4:55743259-55743278 Msa0336360:CDS 40.0%
ATGTCTGTCAACTGAGATAC+AGG - chr2_4:55743347-55743366 None:intergenic 40.0%
TGTATCTCAGTTGACAGACA+TGG + chr2_4:55743346-55743365 Msa0336360:CDS 40.0%
TTGAAGAAGAGACGAGAAGA+AGG + chr2_4:55743411-55743430 Msa0336360:CDS 40.0%
! GCTGCAATTTTGCAAACACA+AGG - chr2_4:55743659-55743678 None:intergenic 40.0%
GATAATAGAGCATGCAAGGA+AGG - chr2_4:55743723-55743742 None:intergenic 40.0%
! TTTCACCGATTATTTCCCCT+AGG + chr2_4:55743781-55743800 Msa0336360:three_prime_UTR 40.0%
TAAGGGTGGTGATCCTGAAA+AGG + chr2_4:55741407-55741426 Msa0336360:CDS 45.0%
! GAATTAGGGTTGTGGGTTGT+TGG + chr2_4:55741539-55741558 Msa0336360:intron 45.0%
TATAGGATCCCCGTAGCTAA+TGG + chr2_4:55741661-55741680 Msa0336360:intron 45.0%
ATAGGATCCCCGTAGCTAAT+GGG + chr2_4:55741662-55741681 Msa0336360:intron 45.0%
AGAGCTTGCACAAGCTTATG+AGG + chr2_4:55742035-55742054 Msa0336360:CDS 45.0%
TCATATACCTCACGCTTCTC+AGG - chr2_4:55742072-55742091 None:intergenic 45.0%
GTATGGTGAAGATGCCCTTA+AGG + chr2_4:55742095-55742114 Msa0336360:CDS 45.0%
AGATGCCCTTAAGGAAGGAA+TGG + chr2_4:55742104-55742123 Msa0336360:CDS 45.0%
GATGCCCTTAAGGAAGGAAT+GGG + chr2_4:55742105-55742124 Msa0336360:CDS 45.0%
ACCACCCATTCCTTCCTTAA+GGG - chr2_4:55742112-55742131 None:intergenic 45.0%
TCCTCCAAAGAGACCTTCAA+GGG - chr2_4:55742353-55742372 None:intergenic 45.0%
ATCCTCCAAAGAGACCTTCA+AGG - chr2_4:55742354-55742373 None:intergenic 45.0%
TAACACATACCCCTTGCACT+TGG - chr2_4:55742429-55742448 None:intergenic 45.0%
! AGTGCAAGGGGTATGTGTTA+TGG + chr2_4:55742429-55742448 Msa0336360:intron 45.0%
! CAAGGGGTATGTGTTATGGA+AGG + chr2_4:55742433-55742452 Msa0336360:intron 45.0%
ACACCCCAAGTTCAAACGAA+AGG + chr2_4:55742937-55742956 Msa0336360:CDS 45.0%
CACCCCAAGTTCAAACGAAA+GGG + chr2_4:55742938-55742957 Msa0336360:CDS 45.0%
CACCCTTTCGTTTGAACTTG+GGG - chr2_4:55742943-55742962 None:intergenic 45.0%
ACCACATAAAGCCTCAGTGA+GGG - chr2_4:55742993-55743012 None:intergenic 45.0%
AACCACATAAAGCCTCAGTG+AGG - chr2_4:55742994-55743013 None:intergenic 45.0%
CCAAATGAGTCAGCGCAAAT+TGG - chr2_4:55743018-55743037 None:intergenic 45.0%
!! CCAATTTGCGCTGACTCATT+TGG + chr2_4:55743015-55743034 Msa0336360:CDS 45.0%
AAGGAATGCCAATGTACCAG+AGG + chr2_4:55743219-55743238 Msa0336360:CDS 45.0%
ATGAAAGGCCTCTGGTACAT+TGG - chr2_4:55743230-55743249 None:intergenic 45.0%
TACCAGAGGCCTTTCATGAA+GGG + chr2_4:55743233-55743252 Msa0336360:CDS 45.0%
GATCAAGAGACAGAGACTCA+GGG - chr2_4:55743289-55743308 None:intergenic 45.0%
TGATCAAGAGACAGAGACTC+AGG - chr2_4:55743290-55743309 None:intergenic 45.0%
TGAGTCTCTGTCTCTTGATC+AGG + chr2_4:55743289-55743308 Msa0336360:CDS 45.0%
TCTGTCTCTTGATCAGGTCA+AGG + chr2_4:55743295-55743314 Msa0336360:CDS 45.0%
TCTTGATCAGGTCAAGGCTT+TGG + chr2_4:55743301-55743320 Msa0336360:CDS 45.0%
! TCAGCAAGAGGCATATGATG+AGG + chr2_4:55743442-55743461 Msa0336360:CDS 45.0%
GATGAGGATGATGAAATGCC+TGG + chr2_4:55743458-55743477 Msa0336360:CDS 45.0%
GTGCTCAGCAGTAATGAGTT+TGG + chr2_4:55743501-55743520 Msa0336360:exon 45.0%
GAGTTTGGAGATGTCCTTCA+TGG + chr2_4:55743516-55743535 Msa0336360:three_prime_UTR 45.0%
!! AGCTTTGTTGGGCAAAACAG+TGG + chr2_4:55743548-55743567 Msa0336360:three_prime_UTR 45.0%
TCAGGATCACCACCCTTATC+AGG - chr2_4:55741405-55741424 None:intergenic 50.0%
GGTGAAGATGCCCTTAAGGA+AGG + chr2_4:55742099-55742118 Msa0336360:CDS 50.0%
CACCACCCATTCCTTCCTTA+AGG - chr2_4:55742113-55742132 None:intergenic 50.0%
CTTAAGGAAGGAATGGGTGG+TGG + chr2_4:55742111-55742130 Msa0336360:CDS 50.0%
TCATCTTTCTTCGGTGGAGG+TGG + chr2_4:55742165-55742184 Msa0336360:CDS 50.0%
! TGGAGGTGGATTTCCTGGTA+TGG + chr2_4:55742179-55742198 Msa0336360:intron 50.0%
GTGGTAGTAGCAGAGGAAGA+AGG + chr2_4:55742300-55742319 Msa0336360:CDS 50.0%
TCACCCCTTGAAGGTCTCTT+TGG + chr2_4:55742346-55742365 Msa0336360:CDS 50.0%
CCTCCAAAGAGACCTTCAAG+GGG - chr2_4:55742352-55742371 None:intergenic 50.0%
TGTCTTGTGCTCCAAGTGCA+AGG + chr2_4:55742415-55742434 Msa0336360:CDS 50.0%
GTCTTGTGCTCCAAGTGCAA+GGG + chr2_4:55742416-55742435 Msa0336360:CDS 50.0%
TCTTGTGCTCCAAGTGCAAG+GGG + chr2_4:55742417-55742436 Msa0336360:CDS 50.0%
!! ATGACCTGTGCTGGTTGTCA+AGG + chr2_4:55742552-55742571 Msa0336360:CDS 50.0%
AGAACCTTGACAACCAGCAC+AGG - chr2_4:55742559-55742578 None:intergenic 50.0%
!! TGTGCTGGTTGTCAAGGTTC+TGG + chr2_4:55742558-55742577 Msa0336360:CDS 50.0%
GTTGAGAAGGGGATGCAGAA+TGG + chr2_4:55742744-55742763 Msa0336360:CDS 50.0%
GGTCAGAAGATTACCTTCCC+TGG + chr2_4:55742765-55742784 Msa0336360:CDS 50.0%
TTCATCAGCTTCACCAGGGA+AGG - chr2_4:55742781-55742800 None:intergenic 50.0%
! GCTGATGAAGCGGTAGGTTT+TGG + chr2_4:55742792-55742811 Msa0336360:intron 50.0%
! TCCCTCACTGAGGCTTTATG+TGG + chr2_4:55742989-55743008 Msa0336360:CDS 50.0%
CAGGCTTGACAACTTCTCCA+GGG - chr2_4:55743069-55743088 None:intergenic 50.0%
GTACCAGAGGCCTTTCATGA+AGG + chr2_4:55743232-55743251 Msa0336360:CDS 50.0%
TGGAGACAATTCTCCCAGCA+AGG + chr2_4:55743321-55743340 Msa0336360:CDS 50.0%
ACTGAGATACAGGCCTTGCT+GGG - chr2_4:55743337-55743356 None:intergenic 50.0%
AACTGAGATACAGGCCTTGC+TGG - chr2_4:55743338-55743357 None:intergenic 50.0%
TCAGTTGACAGACATGGAGC+TGG + chr2_4:55743352-55743371 Msa0336360:CDS 50.0%
GAGGATGATGAAATGCCTGG+TGG + chr2_4:55743461-55743480 Msa0336360:CDS 50.0%
TGTACTCTTTGAGCACCACC+AGG - chr2_4:55743479-55743498 None:intergenic 50.0%
! GAGTGATCCTGAGAAGCGTG+AGG + chr2_4:55742062-55742081 Msa0336360:CDS 55.0%
GCCCTTAAGGAAGGAATGGG+TGG + chr2_4:55742108-55742127 Msa0336360:CDS 55.0%
! AAGGAAGGAATGGGTGGTGG+CGG + chr2_4:55742114-55742133 Msa0336360:CDS 55.0%
! TTCGGTGGAGGTGGATTTCC+TGG + chr2_4:55742174-55742193 Msa0336360:CDS 55.0%
! ACAGGTGGTGGTAGTAGCAG+AGG + chr2_4:55742293-55742312 Msa0336360:intron 55.0%
AGAGGAAGAAGGCAGAGACG+TGG + chr2_4:55742311-55742330 Msa0336360:CDS 55.0%
AAGAAGGCAGAGACGTGGTG+AGG + chr2_4:55742316-55742335 Msa0336360:CDS 55.0%
GGATGTCGTTCACCCCTTGA+AGG + chr2_4:55742337-55742356 Msa0336360:CDS 55.0%
CCCCTTGAAGGTCTCTTTGG+AGG + chr2_4:55742349-55742368 Msa0336360:CDS 55.0%
AGATCGATGCCCACAGTGCA+AGG + chr2_4:55742680-55742699 Msa0336360:CDS 55.0%
GATCGATGCCCACAGTGCAA+GGG + chr2_4:55742681-55742700 Msa0336360:CDS 55.0%
CCTTCTCTCCCTTGCACTGT+GGG - chr2_4:55742692-55742711 None:intergenic 55.0%
ACCTTCTCTCCCTTGCACTG+TGG - chr2_4:55742693-55742712 None:intergenic 55.0%
CCGCTTCATCAGCTTCACCA+GGG - chr2_4:55742785-55742804 None:intergenic 55.0%
CCCTGGTGAAGCTGATGAAG+CGG + chr2_4:55742782-55742801 Msa0336360:CDS 55.0%
ACCGCTTCATCAGCTTCACC+AGG - chr2_4:55742786-55742805 None:intergenic 55.0%
GGTGAAGCTGATGAAGCGGT+AGG + chr2_4:55742786-55742805 Msa0336360:intron 55.0%
TTGCAGCCGGACACAGTTAC+TGG + chr2_4:55742884-55742903 Msa0336360:intron 55.0%
GCACACATTGTCCCTCACTG+AGG + chr2_4:55742979-55742998 Msa0336360:CDS 55.0%
CCAGGCTTGACAACTTCTCC+AGG - chr2_4:55743070-55743089 None:intergenic 55.0%
CCTGGAGAAGTTGTCAAGCC+TGG + chr2_4:55743067-55743086 Msa0336360:CDS 55.0%
TGCCCTTCATGAAAGGCCTC+TGG - chr2_4:55743238-55743257 None:intergenic 55.0%
CATGGAGCTGGATGAGTGTG+AGG + chr2_4:55743364-55743383 Msa0336360:CDS 55.0%
AAGGCAGCAAGCTCAGCAAG+AGG + chr2_4:55743430-55743449 Msa0336360:CDS 55.0%
GGTGCTTCGATGACCTGTGC+TGG + chr2_4:55742543-55742562 Msa0336360:CDS 60.0%
CCCACAGTGCAAGGGAGAGA+AGG + chr2_4:55742689-55742708 Msa0336360:CDS 60.0%
!! GAAGGAATGGGTGGTGGCGG+TGG + chr2_4:55742117-55742136 Msa0336360:CDS 65.0%
!! GGAATGGGTGGTGGCGGTGG+TGG + chr2_4:55742120-55742139 Msa0336360:CDS 70.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 55741160 55743868 55741160 ID=Msa0336360;Name=Msa0336360
chr2_4 mRNA 55741160 55743868 55741160 ID=Msa0336360-mRNA-1;Parent=Msa0336360;Name=Msa0336360-mRNA-1;_AED=0.11;_eAED=0.11;_QI=88|1|1|1|0.83|0.71|7|354|417
chr2_4 exon 55741160 55741191 55741160 ID=Msa0336360-mRNA-1:exon:16924;Parent=Msa0336360-mRNA-1
chr2_4 exon 55741223 55741428 55741223 ID=Msa0336360-mRNA-1:exon:16925;Parent=Msa0336360-mRNA-1
chr2_4 exon 55742030 55742195 55742030 ID=Msa0336360-mRNA-1:exon:16926;Parent=Msa0336360-mRNA-1
chr2_4 exon 55742297 55742438 55742297 ID=Msa0336360-mRNA-1:exon:16927;Parent=Msa0336360-mRNA-1
chr2_4 exon 55742527 55742803 55742527 ID=Msa0336360-mRNA-1:exon:16928;Parent=Msa0336360-mRNA-1
chr2_4 exon 55742890 55743088 55742890 ID=Msa0336360-mRNA-1:exon:16929;Parent=Msa0336360-mRNA-1
chr2_4 exon 55743195 55743868 55743195 ID=Msa0336360-mRNA-1:exon:16930;Parent=Msa0336360-mRNA-1
chr2_4 five_prime_UTR 55741160 55741191 55741160 ID=Msa0336360-mRNA-1:five_prime_utr;Parent=Msa0336360-mRNA-1
chr2_4 five_prime_UTR 55741223 55741278 55741223 ID=Msa0336360-mRNA-1:five_prime_utr;Parent=Msa0336360-mRNA-1
chr2_4 CDS 55741279 55741428 55741279 ID=Msa0336360-mRNA-1:cds;Parent=Msa0336360-mRNA-1
chr2_4 CDS 55742030 55742195 55742030 ID=Msa0336360-mRNA-1:cds;Parent=Msa0336360-mRNA-1
chr2_4 CDS 55742297 55742438 55742297 ID=Msa0336360-mRNA-1:cds;Parent=Msa0336360-mRNA-1
chr2_4 CDS 55742527 55742803 55742527 ID=Msa0336360-mRNA-1:cds;Parent=Msa0336360-mRNA-1
chr2_4 CDS 55742890 55743088 55742890 ID=Msa0336360-mRNA-1:cds;Parent=Msa0336360-mRNA-1
chr2_4 CDS 55743195 55743514 55743195 ID=Msa0336360-mRNA-1:cds;Parent=Msa0336360-mRNA-1
chr2_4 three_prime_UTR 55743515 55743868 55743515 ID=Msa0336360-mRNA-1:three_prime_utr;Parent=Msa0336360-mRNA-1
Gene Sequence

>Msa0336360

ATGTTTGGAAGAGCGCCTAAGAAGAGTGATAACACTAAATACTATGAGATTCTTGGTGTTTCGAAGAATGCTTCTCCTGATGATTTGAAGAAAGCGTATAAAAAAGCTGCGATTAAGAATCATCCTGATAAGGGTGGTGATCCTGAAAAGTTCAAAGAGCTTGCACAAGCTTATGAGGTTTTGAGTGATCCTGAGAAGCGTGAGGTATATGATCAGTATGGTGAAGATGCCCTTAAGGAAGGAATGGGTGGTGGCGGTGGTGGTCACGATCCATTTGACATCTTTTCATCTTTCTTCGGTGGAGGTGGATTTCCTGGTGGTGGTAGTAGCAGAGGAAGAAGGCAGAGACGTGGTGAGGATGTCGTTCACCCCTTGAAGGTCTCTTTGGAGGATCTTTACCTTGGGACATCTAAGAAGCTTTCTCTTTCACGAAATGTCTTGTGCTCCAAGTGCAAGGGGAAAGGATCAAAGTCTGGTGCTTCGATGACCTGTGCTGGTTGTCAAGGTTCTGGTATGAAGATATCTATGAGGCATTTGGGTGCCAATATGATTCAGCAAATGCAACATCCTTGCAATGAATGTAAGGGTACTGGAGAGACAATCAGTGACAAAGATCGATGCCCACAGTGCAAGGGAGAGAAGGTTGTTCAACAGAAGAAAGTACTTGAAGTTCATGTTGAGAAGGGGATGCAGAATGGTCAGAAGATTACCTTCCCTGGTGAAGCTGATGAAGCGCCGGACACAGTTACTGGAGATATCGTCTTTGTACTTCAACAGAAGGAACACCCCAAGTTCAAACGAAAGGGTGAAGATCTTTTTGTAGAGCACACATTGTCCCTCACTGAGGCTTTATGTGGTTTCCAATTTGCGCTGACTCATTTGGATTCTCGCCAACTATTGATTAAATCAAACCCTGGAGAAGTTGTCAAGCCTGATTCATACAAGGCAATCAATGATGAAGGAATGCCAATGTACCAGAGGCCTTTCATGAAGGGCAAGCTTTACATTCACTTCACAGTGGAGTTCCCTGAGTCTCTGTCTCTTGATCAGGTCAAGGCTTTGGAGACAATTCTCCCAGCAAGGCCTGTATCTCAGTTGACAGACATGGAGCTGGATGAGTGTGAGGAGACAACACTTCATGATGTCAACATTGAAGAAGAGACGAGAAGAAGGCAGCAAGCTCAGCAAGAGGCATATGATGAGGATGATGAAATGCCTGGTGGTGCTCAAAGAGTACAATGTGCTCAGCAGTAA
Protein sequence

>Msa0336360

MFGRAPKKSDNTKYYEILGVSKNASPDDLKKAYKKAAIKNHPDKGGDPEKFKELAQAYEVLSDPEKREVYDQYGEDALKEGMGGGGGGHDPFDIFSSFFGGGGFPGGGSSRGRRQRRGEDVVHPLKVSLEDLYLGTSKKLSLSRNVLCSKCKGKGSKSGASMTCAGCQGSGMKISMRHLGANMIQQMQHPCNECKGTGETISDKDRCPQCKGEKVVQQKKVLEVHVEKGMQNGQKITFPGEADEAPDTVTGDIVFVLQQKEHPKFKRKGEDLFVEHTLSLTEALCGFQFALTHLDSRQLLIKSNPGEVVKPDSYKAINDEGMPMYQRPFMKGKLYIHFTVEFPESLSLDQVKALETILPARPVSQLTDMELDECEETTLHDVNIEEETRRRQQAQQEAYDEDDEMPGGAQRVQCAQQ*