AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0332390

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0332390 MtrunA17_Chr2g0314601 99.403 670 4 0 1 670 1 670 0.0 1388
Msa0332390 MtrunA17_Chr8g0359401 89.521 668 69 1 1 668 1 667 0.0 1264
Msa0332390 MtrunA17_Chr1g0147841 74.925 666 155 2 4 669 2 655 0.0 1046
Msa0332390 MtrunA17_Chr4g0057161 82.007 289 52 0 380 668 7 295 2.14e-174 498
Msa0332390 MtrunA17_Chr3g0097081 61.780 191 42 2 85 274 1 161 9.56e-67 221
Msa0332390 MtrunA17_Chr3g0097081 52.893 121 50 1 362 475 137 257 5.86e-32 125
Msa0332390 MtrunA17_Chr2g0327981 30.573 314 189 10 9 312 40 334 5.86e-27 112
Msa0332390 MtrunA17_Chr4g0031131 28.617 311 199 10 9 312 34 328 4.16e-26 110
Msa0332390 MtrunA17_Chr4g0067461 27.619 315 195 11 7 312 111 401 1.96e-25 109
Msa0332390 MtrunA17_Chr8g0380921 26.243 362 218 15 1 327 63 410 9.46e-25 107
Msa0332390 MtrunA17_Chr2g0293521 25.978 358 224 13 1 327 63 410 2.94e-24 105
Msa0332390 MtrunA17_Chr4g0073351 27.193 342 208 9 8 321 96 424 3.07e-22 100
Msa0332390 MtrunA17_Chr4g0011281 26.822 343 208 8 8 321 102 430 2.09e-20 94.7
Msa0332390 MtrunA17_Chr1g0195521 26.524 328 201 13 8 312 119 429 1.01e-19 92.4
Msa0332390 MtrunA17_Chr4g0056941 28.274 336 212 10 7 320 93 421 1.75e-19 91.7
Msa0332390 MtrunA17_Chr5g0398811 26.571 350 222 10 5 326 88 430 2.98e-19 90.9
Msa0332390 MtrunA17_Chr7g0230291 26.608 342 210 8 8 321 83 411 4.06e-19 90.5
Msa0332390 MtrunA17_Chr8g0388261 27.066 351 217 13 7 328 4 344 1.47e-18 87.8
Msa0332390 MtrunA17_Chr4g0076751 24.217 351 225 14 7 327 25 364 1.96e-18 87.8
Msa0332390 MtrunA17_Chr3g0121661 26.647 334 216 10 9 320 101 427 3.25e-17 84.7
Msa0332390 MtrunA17_Chr3g0136511 29.796 245 147 9 1 234 1 231 1.09e-14 77.4
Msa0332390 MtrunA17_Chr1g0208111 24.377 361 218 14 6 318 18 371 1.26e-14 76.3
Msa0332390 MtrunA17_Chr1g0182091 28.372 215 140 7 105 312 4 211 2.63e-14 73.2
Msa0332390 MtrunA17_Chr3g0133771 24.280 243 169 6 7 248 2 230 1.63e-12 69.3
Msa0332390 MtrunA17_Chr4g0027491 23.546 361 221 13 6 318 18 371 2.74e-12 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0332390 AT1G78570.1 88.077 671 76 2 1 669 1 669 0.0 1239
Msa0332390 AT3G14790.2 85.351 669 93 2 1 669 4 667 0.0 1170
Msa0332390 AT3G14790.1 85.351 669 93 2 1 669 1 664 0.0 1170
Msa0332390 AT1G53500.1 85.479 668 93 3 2 669 4 667 0.0 1161
Msa0332390 AT1G63000.1 83.039 283 48 0 387 669 15 297 1.56e-177 507
Msa0332390 AT2G28760.1 30.032 313 192 10 9 312 33 327 4.25e-28 116
Msa0332390 AT2G28760.2 30.032 313 192 10 9 312 33 327 4.25e-28 116
Msa0332390 AT2G28760.3 30.032 313 192 10 9 312 33 327 4.25e-28 116
Msa0332390 AT4G20460.1 27.714 350 212 12 8 327 71 409 7.08e-28 117
Msa0332390 AT2G28760.4 30.032 313 192 10 9 312 64 358 1.02e-27 115
Msa0332390 AT5G59290.4 29.712 313 193 10 9 312 3 297 1.38e-27 114
Msa0332390 AT5G59290.1 29.712 313 193 10 9 312 32 326 1.94e-27 114
Msa0332390 AT5G59290.3 29.712 313 193 10 9 312 25 319 2.23e-27 114
Msa0332390 AT5G59290.2 29.712 313 193 10 9 312 47 341 3.14e-27 114
Msa0332390 AT3G46440.2 29.393 313 194 10 9 312 31 325 4.67e-27 113
Msa0332390 AT3G46440.1 29.393 313 194 10 9 312 31 325 4.67e-27 113
Msa0332390 AT3G53520.1 27.187 320 209 10 9 321 122 424 8.93e-25 108
Msa0332390 AT3G62830.2 27.157 313 200 10 9 312 121 414 1.32e-24 107
Msa0332390 AT3G62830.1 27.157 313 200 10 9 312 121 414 1.32e-24 107
Msa0332390 AT2G47650.1 26.837 313 201 10 9 312 123 416 5.34e-24 105
Msa0332390 AT5G44480.3 26.989 352 210 15 8 326 72 409 1.23e-23 104
Msa0332390 AT5G44480.4 26.989 352 210 15 8 326 72 409 1.23e-23 104
Msa0332390 AT5G44480.2 26.989 352 210 15 8 326 72 409 1.23e-23 104
Msa0332390 AT5G44480.1 26.989 352 210 15 8 326 96 433 1.46e-23 104
Msa0332390 AT2G47650.2 26.498 317 203 10 9 312 123 422 7.41e-23 102
Msa0332390 AT1G30620.3 27.809 356 214 15 4 327 65 409 1.37e-22 101
Msa0332390 AT3G53520.2 26.875 320 208 11 9 321 122 422 1.46e-22 101
Msa0332390 AT1G30620.1 27.809 356 214 15 4 327 66 410 1.48e-22 101
Msa0332390 AT1G30620.6 27.809 356 214 15 4 327 66 410 1.48e-22 101
Msa0332390 AT1G30620.4 27.809 356 214 15 4 327 66 410 1.48e-22 101
Msa0332390 AT1G30620.2 27.809 356 214 15 4 327 66 410 1.48e-22 101
Msa0332390 AT3G53520.4 25.948 343 207 11 9 321 122 447 8.46e-22 99.4
Msa0332390 AT1G63180.1 26.331 357 222 14 1 327 1 346 1.03e-20 94.7
Msa0332390 AT4G00110.1 26.844 339 219 8 8 324 92 423 2.23e-20 94.7
Msa0332390 AT2G45310.1 26.866 335 216 9 9 321 99 426 9.79e-20 92.8
Msa0332390 AT1G02000.1 25.893 336 220 9 8 321 93 421 7.98e-19 90.1
Msa0332390 AT4G30440.1 30.901 233 151 6 8 234 89 317 2.48e-18 88.6
Msa0332390 AT1G64440.1 27.920 351 210 15 8 327 4 342 5.59e-18 86.7
Msa0332390 AT1G30620.5 30.256 195 121 6 4 191 66 252 1.33e-17 84.3
Msa0332390 AT3G53520.3 27.897 233 148 7 8 234 121 339 2.25e-17 84.7
Msa0332390 AT4G23920.1 26.163 344 219 12 7 323 3 338 3.89e-16 80.9
Msa0332390 AT1G63180.2 26.689 296 181 11 1 267 1 289 4.45e-16 80.1
Msa0332390 AT3G23820.1 26.567 335 217 10 8 320 113 440 1.53e-15 80.1
Msa0332390 AT1G47290.1 26.757 370 201 15 3 319 6 358 6.57e-15 77.4
Msa0332390 AT4G12250.1 26.100 341 223 8 3 321 92 425 1.54e-14 77.0
Msa0332390 AT1G47290.2 26.757 370 201 15 3 319 6 358 1.76e-14 76.6
Msa0332390 AT2G26260.1 26.506 249 148 8 2 234 5 234 1.76e-13 73.9
Msa0332390 AT1G73250.1 27.473 182 120 5 151 322 140 319 8.06e-13 70.5

Find 172 sgRNAs with CRISPR-Local

Find 236 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGGAATTCATTCTTCAATT+TGG 0.161081 2_4:-49449335 None:intergenic
CGATCAAAAGTTGAAGGTTT+TGG 0.178673 2_4:+49448114 Msa0332390:CDS
AGGTTCAGGCATTGGGTTTA+AGG 0.187878 2_4:+49448726 Msa0332390:CDS
GCTTACCATTGCCTTGGTTT+TGG 0.211812 2_4:-49448785 None:intergenic
TCTTTGATGGAAAGCAATTC+TGG 0.218557 2_4:-49449355 None:intergenic
TAATTGATAAGTATTATAAT+TGG 0.230179 2_4:+49449559 Msa0332390:three_prime_UTR
GTTGGACATGTTTACAATAT+TGG 0.241068 2_4:+49447965 Msa0332390:CDS
CCCTTCACAAACTTGAAGTT+TGG 0.241790 2_4:-49447404 None:intergenic
TATTGCAGAAGTTGCTGATA+TGG 0.245883 2_4:+49447217 Msa0332390:intron
TTTGCCATCTCAATTGAAAT+AGG 0.250438 2_4:-49449139 None:intergenic
TAGAAAGAACCGGCGAAATT+AGG 0.282017 2_4:-49448760 None:intergenic
AAGAAGTGAAGAGAGAAAAT+AGG 0.287535 2_4:-49446075 None:intergenic
AACCCAATGCCTGAACCTTC+TGG 0.294957 2_4:-49448721 None:intergenic
CTTGATAAGTGATTCTTTGA+TGG 0.295568 2_4:-49449368 None:intergenic
CAAGGGATTCCATATGAATA+TGG 0.304468 2_4:+49448454 Msa0332390:CDS
AATTGATAAGTATTATAATT+GGG 0.306164 2_4:+49449560 Msa0332390:three_prime_UTR
ATGGCGTATGGTAGATCTTA+TGG 0.306165 2_4:+49447746 Msa0332390:CDS
TCCAAACAGCATGACTGTTT+TGG 0.309945 2_4:+49449105 Msa0332390:CDS
CCTCTCAAGTTCTTGATCTA+TGG 0.311827 2_4:+49448385 Msa0332390:CDS
GGTATATCAACAATCCTAAT+TGG 0.322317 2_4:+49448188 Msa0332390:CDS
ACTCATGTCGACAACTCTTT+TGG 0.326250 2_4:+49447506 Msa0332390:CDS
TGAAGTTCCAGATACCTCTT+AGG 0.329274 2_4:-49449168 None:intergenic
TTGCTTAGGTCAGATGAAAT+TGG 0.338530 2_4:-49449031 None:intergenic
TTAGAAGCTTGCAAGGTAAC+TGG 0.349049 2_4:+49447578 Msa0332390:CDS
TTCTTCCCATGTTGTTCTCT+CGG 0.350950 2_4:-49448143 None:intergenic
TTTCCACGTGTTGTGATTAC+CGG 0.356238 2_4:-49447773 None:intergenic
AAGTTAGCCCACTTGAAGTT+TGG 0.358387 2_4:-49449250 None:intergenic
ATCCAGAAGGTTCAGGCATT+GGG 0.362912 2_4:+49448719 Msa0332390:CDS
TGTGAGTTATGTTTGTGTAT+TGG 0.363789 2_4:+49449733 Msa0332390:three_prime_UTR
CTCACATCGCCCCACCAATT+AGG 0.372048 2_4:-49448202 None:intergenic
AGTACCATATATGTTGTTCT+TGG 0.373878 2_4:-49447546 None:intergenic
ACACGTGGAAACAATGTCTA+TGG 0.379199 2_4:+49447785 Msa0332390:CDS
GGAAAAGTTCTTCCGATTCA+TGG 0.380568 2_4:+49447863 Msa0332390:CDS
TCCAAAACAGTCATGCTGTT+TGG 0.382722 2_4:-49449106 None:intergenic
TTTATTGTAACGTGAAATCT+TGG 0.382737 2_4:-49449071 None:intergenic
TTCTTGATCTATGGAAGAAC+TGG 0.390031 2_4:+49448394 Msa0332390:CDS
CATTGCCAAGAGGATAAACT+TGG 0.392228 2_4:-49447837 None:intergenic
AGAGGAACCTAAGAGGTATC+TGG 0.395333 2_4:+49449161 Msa0332390:CDS
TTGTGTTTAGAAGATAATAT+TGG 0.401763 2_4:+49449536 Msa0332390:three_prime_UTR
CCGTACTCTGCAACAAAAGC+TGG 0.402137 2_4:+49447707 Msa0332390:CDS
TTCACCAAGAACAACATATA+TGG 0.402269 2_4:+49447542 Msa0332390:CDS
AATCTACGGAGATTTAGATT+TGG 0.404375 2_4:-49446040 None:intergenic
CTTTGATGGAAAGCAATTCT+GGG 0.407319 2_4:-49449354 None:intergenic
ATGTTGCCAACCGGCTTATC+CGG 0.407727 2_4:+49447297 Msa0332390:CDS
ATCCTCATTACCGGGGCAGC+TGG 0.408875 2_4:+49447260 Msa0332390:CDS
GGTTGGATCGGTGGTTTGCT+TGG 0.414855 2_4:+49448415 Msa0332390:CDS
GTTGGATCGGTGGTTTGCTT+GGG 0.417488 2_4:+49448416 Msa0332390:CDS
ACAGATGAGGATGCGGTTGT+TGG 0.419126 2_4:+49447650 Msa0332390:CDS
TAAACACAACAATAACAGTA+TGG 0.420329 2_4:-49449522 None:intergenic
TCTTGACGATCAAAAGTTGA+AGG 0.421916 2_4:+49448108 Msa0332390:CDS
TAAGTATTATAATTGGGGTT+AGG 0.426960 2_4:+49449566 Msa0332390:three_prime_UTR
GTAAGCACTGATGAGGTCTA+TGG 0.433555 2_4:+49447623 Msa0332390:CDS
AAGTTTGGAGATGATCTTGA+AGG 0.433792 2_4:-49447389 None:intergenic
CGGGAAGACCTAACGTTGAT+TGG 0.437127 2_4:+49448563 Msa0332390:CDS
CATCCAGAAGGTTCAGGCAT+TGG 0.437868 2_4:+49448718 Msa0332390:CDS
TGGTAGATCTTATGGATTGC+CGG 0.438990 2_4:+49447754 Msa0332390:CDS
GAAGAGCCAATGCTCTTTGT+AGG 0.440799 2_4:-49448352 None:intergenic
TTGCGTCTCATGTTGCCAAC+CGG 0.441564 2_4:+49447288 Msa0332390:CDS
ATGACAATGTATGCACTCTC+AGG 0.444077 2_4:+49448999 Msa0332390:CDS
ATTCCATATGAATATGGAAA+AGG 0.444160 2_4:+49448460 Msa0332390:CDS
ATTATAATTGGGGTTAGGTA+TGG 0.447743 2_4:+49449571 Msa0332390:three_prime_UTR
TAGCTAGCTTACCATTGCCT+TGG 0.452476 2_4:-49448791 None:intergenic
CGTTGTTGGGACTTTAACAT+TGG 0.453861 2_4:+49448621 Msa0332390:CDS
GATTCCCAAGTTTATCCTCT+TGG 0.455793 2_4:+49447832 Msa0332390:CDS
GAAGCTCATCCAGAAGGTTC+AGG 0.456647 2_4:+49448712 Msa0332390:CDS
TTCAATGCAGCAGGCGTGAC+GGG 0.457186 2_4:+49448544 Msa0332390:CDS
TTTGAAGTTATCCTTCATAA+GGG 0.460918 2_4:+49447938 Msa0332390:CDS
CCATCCGTGTCAACGTTGTT+GGG 0.465475 2_4:+49448608 Msa0332390:CDS
CGCAATGAATCCAGCTGCCC+CGG 0.466589 2_4:-49447270 None:intergenic
ACCATCCGTGTCAACGTTGT+TGG 0.468379 2_4:+49448607 Msa0332390:CDS
TCGTTGTGGCTCACGGCGCC+AGG 0.468437 2_4:-49449196 None:intergenic
AAGTATCTCTGATCATTAAA+CGG 0.469948 2_4:-49448085 None:intergenic
ATCTGGAACTTCACAAATCC+TGG 0.470303 2_4:+49449178 Msa0332390:CDS
TTACAATATTGGAACTAAGA+AGG 0.484035 2_4:+49447976 Msa0332390:CDS
CGTACTCTGCAACAAAAGCT+GGG 0.487722 2_4:+49447708 Msa0332390:CDS
GTTGCAGAGTACGGATTTGT+TGG 0.489137 2_4:-49447698 None:intergenic
GAGATGATCTTGAAGGAATG+AGG 0.489742 2_4:-49447382 None:intergenic
ATTGATAAGTATTATAATTG+GGG 0.495923 2_4:+49449561 Msa0332390:three_prime_UTR
TCTTCCTATTTCAATTGAGA+TGG 0.498900 2_4:+49449135 Msa0332390:CDS
TCTTGCAGCAACTATCACTT+TGG 0.499126 2_4:-49449290 None:intergenic
ATCCTTCATAAGGGAGAGGT+TGG 0.502469 2_4:+49447947 Msa0332390:CDS
AGACCTCATCAGTGCTTACA+TGG 0.502767 2_4:-49447619 None:intergenic
TTCCATATGAATATGGAAAA+GGG 0.506901 2_4:+49448461 Msa0332390:CDS
CAAGCTCTTCTCAATTGATC+CGG 0.510856 2_4:+49448033 Msa0332390:CDS
TAGCATAGTTCATCATCAAA+AGG 0.516883 2_4:-49448666 None:intergenic
GTTCTTCCGATTCATGGTGA+TGG 0.517259 2_4:+49447869 Msa0332390:CDS
TGCAAGAAGCAACAATGAAA+TGG 0.518316 2_4:+49449306 Msa0332390:CDS
TGAAATTGCGTGGATTGCTT+AGG 0.528340 2_4:-49449045 None:intergenic
TCCAAACTTCAAGTTTGTGA+AGG 0.531089 2_4:+49447403 Msa0332390:CDS
TTGATGATGAACTATGCTAC+CGG 0.533136 2_4:+49448670 Msa0332390:CDS
GTTACCTTGTGAGCTTGCTA+CGG 0.534317 2_4:+49449648 Msa0332390:three_prime_UTR
GGGAAATTGTGTGAGAAACA+AGG 0.534485 2_4:+49448436 Msa0332390:CDS
TTTGAACCATCACCATGAAT+CGG 0.534712 2_4:-49447875 None:intergenic
TTTATCCTCTTGGCAATGCA+AGG 0.535363 2_4:+49447842 Msa0332390:CDS
TATCAACAATCCTAATTGGT+GGG 0.540325 2_4:+49448192 Msa0332390:CDS
GTCCAACCTCTCCCTTATGA+AGG 0.542052 2_4:-49447949 None:intergenic
TGATCTATGGAAGAACTGGT+TGG 0.542351 2_4:+49448398 Msa0332390:CDS
ATTGTTGATATACCAGTCCA+TGG 0.546234 2_4:-49448179 None:intergenic
GAGAGAACAACATGGGAAGA+AGG 0.546603 2_4:+49448145 Msa0332390:CDS
CCAAACTTCAAGTTTGTGAA+GGG 0.547634 2_4:+49447404 Msa0332390:CDS
GGAAGAACTGGTTGGATCGG+TGG 0.548201 2_4:+49448406 Msa0332390:CDS
CCGAAAATGTTAATGATGCC+TGG 0.549298 2_4:+49448244 Msa0332390:CDS
GTTAATGATGCCTGGTGGGA+TGG 0.550016 2_4:+49448252 Msa0332390:CDS
CGGGATAGTTCCGGATAAGC+CGG 0.551283 2_4:-49447307 None:intergenic
GATTCACACCAATCAACGTT+AGG 0.551310 2_4:-49448571 None:intergenic
ATCCAGCTGCCCCGGTAATG+AGG 0.552923 2_4:-49447262 None:intergenic
GGAGCTGAAATCGAAATCTA+CGG 0.555069 2_4:-49446054 None:intergenic
GGATGGAGAGACATTTCAAT+GGG 0.556466 2_4:+49448269 Msa0332390:CDS
TTCTTTCTATTCCAAAACCA+AGG 0.556902 2_4:+49448774 Msa0332390:CDS
CCCAACAACGTTGACACGGA+TGG 0.559674 2_4:-49448608 None:intergenic
TTTCAATGCAGCAGGCGTGA+CGG 0.562169 2_4:+49448543 Msa0332390:CDS
AGTTATCCTTCATAAGGGAG+AGG 0.562307 2_4:+49447943 Msa0332390:CDS
GGAAATTGTGTGAGAAACAA+GGG 0.564756 2_4:+49448437 Msa0332390:CDS
CGATAGACTCAGTGATGAGA+AGG 0.564861 2_4:-49447457 None:intergenic
CTATTCCAAAACCAAGGCAA+TGG 0.566752 2_4:+49448780 Msa0332390:CDS
AAATGTCTCTCCATCCCACC+AGG 0.569236 2_4:-49448262 None:intergenic
GGGATGGAGAGACATTTCAA+TGG 0.570949 2_4:+49448268 Msa0332390:CDS
TCATGTCTTCAATATCAGAA+TGG 0.573520 2_4:-49449680 None:intergenic
GAAATGCTTGTCATGGCGTA+TGG 0.574187 2_4:+49447734 Msa0332390:CDS
GAAGACACTCCTAATTTCGC+CGG 0.574480 2_4:+49448751 Msa0332390:CDS
ACATTGATCCAAACTTCAAG+TGG 0.579567 2_4:+49449242 Msa0332390:CDS
CTCAAGTACTAATACTCGAA+TGG 0.579731 2_4:+49448321 Msa0332390:CDS
TGTCTTATTAGAAGCTTGCA+AGG 0.580370 2_4:+49447571 Msa0332390:CDS
ATATCAACAATCCTAATTGG+TGG 0.585366 2_4:+49448191 Msa0332390:CDS
TGGAGAGACAGATGAGGATG+CGG 0.589535 2_4:+49447643 Msa0332390:CDS
TATGGAAGAACTGGTTGGAT+CGG 0.593876 2_4:+49448403 Msa0332390:CDS
GAGCCAATGCTCTTTGTAGG+TGG 0.595767 2_4:-49448349 None:intergenic
GTTCCACCTACAAAGAGCAT+TGG 0.596551 2_4:+49448346 Msa0332390:CDS
TGAGGATCGTTCATCACTCG+TGG 0.597692 2_4:+49448489 Msa0332390:CDS
CCATAGATCAAGAACTTGAG+AGG 0.598084 2_4:-49448385 None:intergenic
ATCAACAATCCTAATTGGTG+GGG 0.599048 2_4:+49448193 Msa0332390:CDS
GCTTGAAGAAAACCATGGAC+TGG 0.603416 2_4:+49448167 Msa0332390:CDS
GTATTAGTACTTGAGACAAA+CGG 0.603642 2_4:-49448313 None:intergenic
GAGAATCTCGTTGTGGCTCA+CGG 0.604501 2_4:-49449203 None:intergenic
TGGGGCTGAAATGCTTGTCA+TGG 0.606750 2_4:+49447727 Msa0332390:CDS
GCTGCATTGAAAATATGAGT+TGG 0.607206 2_4:-49448532 None:intergenic
GTTATCTAGCACTTATGCCG+TGG 0.611201 2_4:+49449810 Msa0332390:three_prime_UTR
ATCATACTCGAATATGCACC+CGG 0.611680 2_4:-49448689 None:intergenic
AACAGTAATCGAGTTTGTCG+AGG 0.615310 2_4:-49447346 None:intergenic
TGATGATGAACTATGCTACC+GGG 0.618950 2_4:+49448671 Msa0332390:CDS
ATGGCAAAGAGGAACCTAAG+AGG 0.622901 2_4:+49449154 Msa0332390:CDS
TATGATGAAGCTCATCCAGA+AGG 0.624291 2_4:+49448706 Msa0332390:CDS
ATATTGGAACTAAGAAGGAA+AGG 0.626669 2_4:+49447981 Msa0332390:CDS
TTTCAATTGAGATGGCAAAG+AGG 0.627252 2_4:+49449143 Msa0332390:CDS
CATCCATGTAAGCACTGATG+AGG 0.632531 2_4:+49447616 Msa0332390:CDS
AAGTCCCAACAACGTTGACA+CGG 0.632986 2_4:-49448612 None:intergenic
ATAGTTCCGGATAAGCCGGT+TGG 0.636641 2_4:-49447303 None:intergenic
AATTGGTGGGGCGATGTGAG+TGG 0.640742 2_4:+49448205 Msa0332390:CDS
ATCTTGGTGATGAAATTGCG+TGG 0.642464 2_4:-49449055 None:intergenic
ATATGGAAAAGGGCGTCTTG+AGG 0.643401 2_4:+49448471 Msa0332390:CDS
TGATGAGAAGGTAGTTGACA+AGG 0.649249 2_4:-49447445 None:intergenic
AGAAGGCTTGAAGAAAACCA+TGG 0.651189 2_4:+49448162 Msa0332390:CDS
TGACAATGTATGCACTCTCA+GGG 0.651558 2_4:+49449000 Msa0332390:CDS
TTAACCGTAGCAAGCTCACA+AGG 0.651715 2_4:-49449652 None:intergenic
GGTCTATGGAGAGACAGATG+AGG 0.654888 2_4:+49447637 Msa0332390:CDS
GAAGAGCTTGCATATATCAG+TGG 0.655262 2_4:-49448020 None:intergenic
CATTGATCCAAACTTCAAGT+GGG 0.662099 2_4:+49449243 Msa0332390:CDS
TTGCCGGTAATCACAACACG+TGG 0.662580 2_4:+49447770 Msa0332390:CDS
TAGATCAAGAACTTGAGAGG+AGG 0.663040 2_4:-49448382 None:intergenic
ACATCTCGAGAATCTCGTTG+TGG 0.664572 2_4:-49449210 None:intergenic
AAGTACTAATACTCGAATGG+TGG 0.665787 2_4:+49448324 Msa0332390:CDS
AAATGTTAATGATGCCTGGT+GGG 0.666092 2_4:+49448248 Msa0332390:CDS
ATTGCCAAGAGGATAAACTT+GGG 0.666126 2_4:-49447836 None:intergenic
TCTGTATTGTGAAGATGTTG+CGG 0.669863 2_4:+49447910 Msa0332390:CDS
GTACTCTGCAACAAAAGCTG+GGG 0.681769 2_4:+49447709 Msa0332390:CDS
TTGGTCCGAGAGAACAACAT+GGG 0.688575 2_4:+49448138 Msa0332390:CDS
GAAGTAGCTGAGAAGCCTCG+TGG 0.688718 2_4:-49447676 None:intergenic
ATGGTGATGGTTCAAATGTG+AGG 0.706627 2_4:+49447882 Msa0332390:CDS
GTTGGTCCGAGAGAACAACA+TGG 0.719943 2_4:+49448137 Msa0332390:CDS
GTATTGTGAAGATGTTGCGG+AGG 0.726140 2_4:+49447913 Msa0332390:CDS
TGCGGTTGTTGGAAACCACG+AGG 0.732893 2_4:+49447661 Msa0332390:CDS
AAAATGTTAATGATGCCTGG+TGG 0.736377 2_4:+49448247 Msa0332390:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTGATAAGTATTATAAT+TGG + chr2_4:49449559-49449578 Msa0332390:three_prime_UTR 10.0%
!! AATTGATAAGTATTATAATT+GGG + chr2_4:49449560-49449579 Msa0332390:three_prime_UTR 10.0%
!! ATTCATAATCATAATGAAAA+TGG - chr2_4:49448923-49448942 None:intergenic 15.0%
!! ATTGATAAGTATTATAATTG+GGG + chr2_4:49449561-49449580 Msa0332390:three_prime_UTR 15.0%
!!! ACTAGATTTTTATTTAAATG+AGG - chr2_4:49449790-49449809 None:intergenic 15.0%
!! GAACAAAAAAACTTACAAAT+TGG - chr2_4:49446113-49446132 None:intergenic 20.0%
!!! TTGTTTTTACTGAATTTTAG+TGG + chr2_4:49446531-49446550 Msa0332390:intron 20.0%
!!! ATTGATATTTTGACTTGTTA+AGG + chr2_4:49446596-49446615 Msa0332390:intron 20.0%
!!! TAGAAAAAAGTTTTTCTGAT+TGG + chr2_4:49446977-49446996 Msa0332390:intron 20.0%
!! ACATTCAATTTCTATACTAA+AGG - chr2_4:49447064-49447083 None:intergenic 20.0%
!! ACAGAAACTAAAATAAAACA+TGG - chr2_4:49447126-49447145 None:intergenic 20.0%
!!! TTTAGTTTCTGTGATTATTT+AGG + chr2_4:49447134-49447153 Msa0332390:intron 20.0%
!!! ATTTTGATTATATTTGTCTG+TGG + chr2_4:49447160-49447179 Msa0332390:intron 20.0%
!! AAAAAAAAAAGGAATCAGAA+TGG - chr2_4:49448845-49448864 None:intergenic 20.0%
!! TTGTGTTTAGAAGATAATAT+TGG + chr2_4:49449536-49449555 Msa0332390:three_prime_UTR 20.0%
!!! AATGAGTGTTTTTTATGATC+TGG + chr2_4:49446239-49446258 Msa0332390:intron 25.0%
!!! ATGAGTGTTTTTTATGATCT+GGG + chr2_4:49446240-49446259 Msa0332390:intron 25.0%
! TTTCATCATAAACAATCACA+TGG - chr2_4:49446328-49446347 None:intergenic 25.0%
!!! TGGATGTGTTTTTATTGTTT+AGG + chr2_4:49446463-49446482 Msa0332390:intron 25.0%
!!! TTTTACTGAATTTTAGTGGT+TGG + chr2_4:49446535-49446554 Msa0332390:intron 25.0%
!!! ATTTTGACTTGTTAAGGAAA+TGG + chr2_4:49446602-49446621 Msa0332390:intron 25.0%
!! TCAAGAAAATGTGTTACTAT+AGG + chr2_4:49446626-49446645 Msa0332390:intron 25.0%
! GATCTGTATCTTAAGATAAA+TGG - chr2_4:49446715-49446734 None:intergenic 25.0%
! ATAGTAAACACAGTAAGTTT+AGG - chr2_4:49446805-49446824 None:intergenic 25.0%
! ATAAGAAGTTAACAAGATCA+TGG - chr2_4:49447026-49447045 None:intergenic 25.0%
!!! ACTAAAGGTAAATTGACTTT+TGG - chr2_4:49447049-49447068 None:intergenic 25.0%
!!! TTTTGAAGTTATCCTTCATA+AGG + chr2_4:49447937-49447956 Msa0332390:CDS 25.0%
!! TTTGAAGTTATCCTTCATAA+GGG + chr2_4:49447938-49447957 Msa0332390:CDS 25.0%
! TTACAATATTGGAACTAAGA+AGG + chr2_4:49447976-49447995 Msa0332390:CDS 25.0%
! AAGTATCTCTGATCATTAAA+CGG - chr2_4:49448088-49448107 None:intergenic 25.0%
! ATTCCATATGAATATGGAAA+AGG + chr2_4:49448460-49448479 Msa0332390:CDS 25.0%
! TTCCATATGAATATGGAAAA+GGG + chr2_4:49448461-49448480 Msa0332390:CDS 25.0%
! CCTCGATTTCAAAAAAAAAA+AGG - chr2_4:49448856-49448875 None:intergenic 25.0%
!!! CCTTTTTTTTTTTGAAATCG+AGG + chr2_4:49448853-49448872 Msa0332390:intron 25.0%
! TTTATTGTAACGTGAAATCT+TGG - chr2_4:49449074-49449093 None:intergenic 25.0%
! TAAACACAACAATAACAGTA+TGG - chr2_4:49449525-49449544 None:intergenic 25.0%
! TAAGTATTATAATTGGGGTT+AGG + chr2_4:49449566-49449585 Msa0332390:three_prime_UTR 25.0%
AATCTACGGAGATTTAGATT+TGG - chr2_4:49446043-49446062 None:intergenic 30.0%
AAGAAGTGAAGAGAGAAAAT+AGG - chr2_4:49446078-49446097 None:intergenic 30.0%
TTCTTTCATTGATCTTGTGA+TGG + chr2_4:49446166-49446185 Msa0332390:intron 30.0%
CAAAATCCAAATCATCAACT+GGG - chr2_4:49446192-49446211 None:intergenic 30.0%
ACAAAATCCAAATCATCAAC+TGG - chr2_4:49446193-49446212 None:intergenic 30.0%
GAATCTGATTCATGAAATTG+TGG + chr2_4:49446443-49446462 Msa0332390:intron 30.0%
AAAACTCACACAAGTTAAAG+TGG - chr2_4:49446671-49446690 None:intergenic 30.0%
TGGGATTCATCATAATGAAT+CGG - chr2_4:49446761-49446780 None:intergenic 30.0%
TCGAGTCATTACAATGAATT+GGG - chr2_4:49446780-49446799 None:intergenic 30.0%
ATCGAGTCATTACAATGAAT+TGG - chr2_4:49446781-49446800 None:intergenic 30.0%
TTCACCAAGAACAACATATA+TGG + chr2_4:49447542-49447561 Msa0332390:CDS 30.0%
AGTACCATATATGTTGTTCT+TGG - chr2_4:49447549-49447568 None:intergenic 30.0%
!! ACTTTTCAGGAAACTGATTT+GGG - chr2_4:49447811-49447830 None:intergenic 30.0%
!! AACTTTTCAGGAAACTGATT+TGG - chr2_4:49447812-49447831 None:intergenic 30.0%
GTTGGACATGTTTACAATAT+TGG + chr2_4:49447965-49447984 Msa0332390:CDS 30.0%
ATATTGGAACTAAGAAGGAA+AGG + chr2_4:49447981-49448000 Msa0332390:CDS 30.0%
!! ACAAATTTTATGCTAGTCTC+CGG - chr2_4:49448055-49448074 None:intergenic 30.0%
! GATCAAAAGTTGAAGGTTTT+GGG + chr2_4:49448115-49448134 Msa0332390:CDS 30.0%
GGTATATCAACAATCCTAAT+TGG + chr2_4:49448188-49448207 Msa0332390:CDS 30.0%
ATATCAACAATCCTAATTGG+TGG + chr2_4:49448191-49448210 Msa0332390:CDS 30.0%
TATCAACAATCCTAATTGGT+GGG + chr2_4:49448192-49448211 Msa0332390:CDS 30.0%
! ATCATTAACATTTTCGGATG+AGG - chr2_4:49448241-49448260 None:intergenic 30.0%
GTATTAGTACTTGAGACAAA+CGG - chr2_4:49448316-49448335 None:intergenic 30.0%
TAGCATAGTTCATCATCAAA+AGG - chr2_4:49448669-49448688 None:intergenic 30.0%
TTCTTTCTATTCCAAAACCA+AGG + chr2_4:49448774-49448793 Msa0332390:CDS 30.0%
! TTGAACAAGATTTGTTCAAC+AGG - chr2_4:49448894-49448913 None:intergenic 30.0%
TTAATCTGTGAATGTTTCTC+AGG + chr2_4:49448955-49448974 Msa0332390:intron 30.0%
TCTTCCTATTTCAATTGAGA+TGG + chr2_4:49449135-49449154 Msa0332390:CDS 30.0%
TTTGCCATCTCAATTGAAAT+AGG - chr2_4:49449142-49449161 None:intergenic 30.0%
TGGGAATTCATTCTTCAATT+TGG - chr2_4:49449338-49449357 None:intergenic 30.0%
CTTGATAAGTGATTCTTTGA+TGG - chr2_4:49449371-49449390 None:intergenic 30.0%
! GCTACCAAATTTTTGTGTTT+TGG + chr2_4:49449461-49449480 Msa0332390:three_prime_UTR 30.0%
GTAGCCAAAACACAAAAATT+TGG - chr2_4:49449468-49449487 None:intergenic 30.0%
ATTATAATTGGGGTTAGGTA+TGG + chr2_4:49449571-49449590 Msa0332390:three_prime_UTR 30.0%
TCATGTCTTCAATATCAGAA+TGG - chr2_4:49449683-49449702 None:intergenic 30.0%
TGTGAGTTATGTTTGTGTAT+TGG + chr2_4:49449733-49449752 Msa0332390:three_prime_UTR 30.0%
ATTGACTACACAAAGTTGAC+AGG - chr2_4:49446840-49446859 None:intergenic 35.0%
TATTGCAGAAGTTGCTGATA+TGG + chr2_4:49447217-49447236 Msa0332390:intron 35.0%
!!! GGTAATGAGGATGTTTTTTG+GGG - chr2_4:49447252-49447271 None:intergenic 35.0%
!!! CGGTAATGAGGATGTTTTTT+GGG - chr2_4:49447253-49447272 None:intergenic 35.0%
CCAAAAAACATCCTCATTAC+CGG + chr2_4:49447251-49447270 Msa0332390:CDS 35.0%
CAAAAAACATCCTCATTACC+GGG + chr2_4:49447252-49447271 Msa0332390:CDS 35.0%
AAAAAACATCCTCATTACCG+GGG + chr2_4:49447253-49447272 Msa0332390:CDS 35.0%
! AGGACAACAATTTTGTAGTC+GGG - chr2_4:49447329-49447348 None:intergenic 35.0%
! GAGGACAACAATTTTGTAGT+CGG - chr2_4:49447330-49447349 None:intergenic 35.0%
AAGTTTGGAGATGATCTTGA+AGG - chr2_4:49447392-49447411 None:intergenic 35.0%
! TCCAAACTTCAAGTTTGTGA+AGG + chr2_4:49447403-49447422 Msa0332390:CDS 35.0%
! CCAAACTTCAAGTTTGTGAA+GGG + chr2_4:49447404-49447423 Msa0332390:CDS 35.0%
! TGTCTTATTAGAAGCTTGCA+AGG + chr2_4:49447571-49447590 Msa0332390:CDS 35.0%
! AACTTGGGAATCAACTTTTC+AGG - chr2_4:49447824-49447843 None:intergenic 35.0%
ATTGCCAAGAGGATAAACTT+GGG - chr2_4:49447839-49447858 None:intergenic 35.0%
TTTGAACCATCACCATGAAT+CGG - chr2_4:49447878-49447897 None:intergenic 35.0%
TCTGTATTGTGAAGATGTTG+CGG + chr2_4:49447910-49447929 Msa0332390:CDS 35.0%
TCTTGACGATCAAAAGTTGA+AGG + chr2_4:49448108-49448127 Msa0332390:CDS 35.0%
! CGATCAAAAGTTGAAGGTTT+TGG + chr2_4:49448114-49448133 Msa0332390:CDS 35.0%
!!! AAAAGTTGAAGGTTTTGGGT+TGG + chr2_4:49448119-49448138 Msa0332390:CDS 35.0%
ATTGTTGATATACCAGTCCA+TGG - chr2_4:49448182-49448201 None:intergenic 35.0%
ATCAACAATCCTAATTGGTG+GGG + chr2_4:49448193-49448212 Msa0332390:CDS 35.0%
! CCAGGCATCATTAACATTTT+CGG - chr2_4:49448247-49448266 None:intergenic 35.0%
AAAATGTTAATGATGCCTGG+TGG + chr2_4:49448247-49448266 Msa0332390:CDS 35.0%
AAATGTTAATGATGCCTGGT+GGG + chr2_4:49448248-49448267 Msa0332390:CDS 35.0%
CTCAAGTACTAATACTCGAA+TGG + chr2_4:49448321-49448340 Msa0332390:CDS 35.0%
AAGTACTAATACTCGAATGG+TGG + chr2_4:49448324-49448343 Msa0332390:CDS 35.0%
TTCTTGATCTATGGAAGAAC+TGG + chr2_4:49448394-49448413 Msa0332390:CDS 35.0%
GGAAATTGTGTGAGAAACAA+GGG + chr2_4:49448437-49448456 Msa0332390:CDS 35.0%
CAAGGGATTCCATATGAATA+TGG + chr2_4:49448454-49448473 Msa0332390:CDS 35.0%
GCTGCATTGAAAATATGAGT+TGG - chr2_4:49448535-49448554 None:intergenic 35.0%
! ACTCATATTTTCAATGCAGC+AGG + chr2_4:49448535-49448554 Msa0332390:CDS 35.0%
!! TTGATGATGAACTATGCTAC+CGG + chr2_4:49448670-49448689 Msa0332390:CDS 35.0%
!!! GGTTTTGGAATAGAAAGAAC+CGG - chr2_4:49448773-49448792 None:intergenic 35.0%
TTGCTTAGGTCAGATGAAAT+TGG - chr2_4:49449034-49449053 None:intergenic 35.0%
TTTCAATTGAGATGGCAAAG+AGG + chr2_4:49449143-49449162 Msa0332390:CDS 35.0%
ACATTGATCCAAACTTCAAG+TGG + chr2_4:49449242-49449261 Msa0332390:CDS 35.0%
CATTGATCCAAACTTCAAGT+GGG + chr2_4:49449243-49449262 Msa0332390:CDS 35.0%
TGCAAGAAGCAACAATGAAA+TGG + chr2_4:49449306-49449325 Msa0332390:CDS 35.0%
CTTTGATGGAAAGCAATTCT+GGG - chr2_4:49449357-49449376 None:intergenic 35.0%
TCTTTGATGGAAAGCAATTC+TGG - chr2_4:49449358-49449377 None:intergenic 35.0%
GGAGCTGAAATCGAAATCTA+CGG - chr2_4:49446057-49446076 None:intergenic 40.0%
AAAAACCAGAGCAAGAGTGA+GGG - chr2_4:49446135-49446154 None:intergenic 40.0%
AAAAAACCAGAGCAAGAGTG+AGG - chr2_4:49446136-49446155 None:intergenic 40.0%
CACAAAGTTGACAGGAAAAG+TGG - chr2_4:49446832-49446851 None:intergenic 40.0%
!!! CCGGTAATGAGGATGTTTTT+TGG - chr2_4:49447254-49447273 None:intergenic 40.0%
! TTTTGTAGTCGGGATAGTTC+CGG - chr2_4:49447319-49447338 None:intergenic 40.0%
AACAGTAATCGAGTTTGTCG+AGG - chr2_4:49447349-49447368 None:intergenic 40.0%
GAGATGATCTTGAAGGAATG+AGG - chr2_4:49447385-49447404 None:intergenic 40.0%
CCCTTCACAAACTTGAAGTT+TGG - chr2_4:49447407-49447426 None:intergenic 40.0%
TGATGAGAAGGTAGTTGACA+AGG - chr2_4:49447448-49447467 None:intergenic 40.0%
! ACTCATGTCGACAACTCTTT+TGG + chr2_4:49447506-49447525 Msa0332390:CDS 40.0%
TTAGAAGCTTGCAAGGTAAC+TGG + chr2_4:49447578-49447597 Msa0332390:CDS 40.0%
ATGGCGTATGGTAGATCTTA+TGG + chr2_4:49447746-49447765 Msa0332390:CDS 40.0%
! TGGTAGATCTTATGGATTGC+CGG + chr2_4:49447754-49447773 Msa0332390:CDS 40.0%
TTTCCACGTGTTGTGATTAC+CGG - chr2_4:49447776-49447795 None:intergenic 40.0%
ACACGTGGAAACAATGTCTA+TGG + chr2_4:49447785-49447804 Msa0332390:CDS 40.0%
GATTCCCAAGTTTATCCTCT+TGG + chr2_4:49447832-49447851 Msa0332390:CDS 40.0%
CATTGCCAAGAGGATAAACT+TGG - chr2_4:49447840-49447859 None:intergenic 40.0%
TTTATCCTCTTGGCAATGCA+AGG + chr2_4:49447842-49447861 Msa0332390:CDS 40.0%
GGAAAAGTTCTTCCGATTCA+TGG + chr2_4:49447863-49447882 Msa0332390:CDS 40.0%
!! ATGGTGATGGTTCAAATGTG+AGG + chr2_4:49447882-49447901 Msa0332390:CDS 40.0%
AGTTATCCTTCATAAGGGAG+AGG + chr2_4:49447943-49447962 Msa0332390:CDS 40.0%
GAAGAGCTTGCATATATCAG+TGG - chr2_4:49448023-49448042 None:intergenic 40.0%
CAAGCTCTTCTCAATTGATC+CGG + chr2_4:49448033-49448052 Msa0332390:CDS 40.0%
TTCTTCCCATGTTGTTCTCT+CGG - chr2_4:49448146-49448165 None:intergenic 40.0%
AGAAGGCTTGAAGAAAACCA+TGG + chr2_4:49448162-49448181 Msa0332390:CDS 40.0%
CCGAAAATGTTAATGATGCC+TGG + chr2_4:49448244-49448263 Msa0332390:CDS 40.0%
GGATGGAGAGACATTTCAAT+GGG + chr2_4:49448269-49448288 Msa0332390:CDS 40.0%
TAGATCAAGAACTTGAGAGG+AGG - chr2_4:49448385-49448404 None:intergenic 40.0%
CCATAGATCAAGAACTTGAG+AGG - chr2_4:49448388-49448407 None:intergenic 40.0%
CCTCTCAAGTTCTTGATCTA+TGG + chr2_4:49448385-49448404 Msa0332390:CDS 40.0%
TGATCTATGGAAGAACTGGT+TGG + chr2_4:49448398-49448417 Msa0332390:CDS 40.0%
TATGGAAGAACTGGTTGGAT+CGG + chr2_4:49448403-49448422 Msa0332390:CDS 40.0%
GGGAAATTGTGTGAGAAACA+AGG + chr2_4:49448436-49448455 Msa0332390:CDS 40.0%
! CGCCCTTTTCCATATTCATA+TGG - chr2_4:49448466-49448485 None:intergenic 40.0%
GATTCACACCAATCAACGTT+AGG - chr2_4:49448574-49448593 None:intergenic 40.0%
CGTTGTTGGGACTTTAACAT+TGG + chr2_4:49448621-49448640 Msa0332390:CDS 40.0%
! TGATGATGAACTATGCTACC+GGG + chr2_4:49448671-49448690 Msa0332390:CDS 40.0%
ATCATACTCGAATATGCACC+CGG - chr2_4:49448692-49448711 None:intergenic 40.0%
TATGATGAAGCTCATCCAGA+AGG + chr2_4:49448706-49448725 Msa0332390:CDS 40.0%
TAGAAAGAACCGGCGAAATT+AGG - chr2_4:49448763-49448782 None:intergenic 40.0%
CTATTCCAAAACCAAGGCAA+TGG + chr2_4:49448780-49448799 Msa0332390:CDS 40.0%
ATGACAATGTATGCACTCTC+AGG + chr2_4:49448999-49449018 Msa0332390:CDS 40.0%
TGACAATGTATGCACTCTCA+GGG + chr2_4:49449000-49449019 Msa0332390:CDS 40.0%
! TGAAATTGCGTGGATTGCTT+AGG - chr2_4:49449048-49449067 None:intergenic 40.0%
!! ATCTTGGTGATGAAATTGCG+TGG - chr2_4:49449058-49449077 None:intergenic 40.0%
! TCCAAACAGCATGACTGTTT+TGG + chr2_4:49449105-49449124 Msa0332390:CDS 40.0%
TCCAAAACAGTCATGCTGTT+TGG - chr2_4:49449109-49449128 None:intergenic 40.0%
TGAAGTTCCAGATACCTCTT+AGG - chr2_4:49449171-49449190 None:intergenic 40.0%
! ATCTGGAACTTCACAAATCC+TGG + chr2_4:49449178-49449197 Msa0332390:CDS 40.0%
AAGTTAGCCCACTTGAAGTT+TGG - chr2_4:49449253-49449272 None:intergenic 40.0%
TCTTGCAGCAACTATCACTT+TGG - chr2_4:49449293-49449312 None:intergenic 40.0%
!! TGATGGCCCAGTTGATGATT+TGG + chr2_4:49446183-49446202 Msa0332390:intron 45.0%
CGATAGACTCAGTGATGAGA+AGG - chr2_4:49447460-49447479 None:intergenic 45.0%
!! CATCCATGTAAGCACTGATG+AGG + chr2_4:49447616-49447635 Msa0332390:CDS 45.0%
AGACCTCATCAGTGCTTACA+TGG - chr2_4:49447622-49447641 None:intergenic 45.0%
!! GTAAGCACTGATGAGGTCTA+TGG + chr2_4:49447623-49447642 Msa0332390:CDS 45.0%
GTTGCAGAGTACGGATTTGT+TGG - chr2_4:49447701-49447720 None:intergenic 45.0%
! CCAGCTTTTGTTGCAGAGTA+CGG - chr2_4:49447710-49447729 None:intergenic 45.0%
CGTACTCTGCAACAAAAGCT+GGG + chr2_4:49447708-49447727 Msa0332390:CDS 45.0%
GTACTCTGCAACAAAAGCTG+GGG + chr2_4:49447709-49447728 Msa0332390:CDS 45.0%
GAAATGCTTGTCATGGCGTA+TGG + chr2_4:49447734-49447753 Msa0332390:CDS 45.0%
! CTTTTCCTTGCATTGCCAAG+AGG - chr2_4:49447850-49447869 None:intergenic 45.0%
GTTCTTCCGATTCATGGTGA+TGG + chr2_4:49447869-49447888 Msa0332390:CDS 45.0%
GTATTGTGAAGATGTTGCGG+AGG + chr2_4:49447913-49447932 Msa0332390:CDS 45.0%
ATCCTTCATAAGGGAGAGGT+TGG + chr2_4:49447947-49447966 Msa0332390:CDS 45.0%
TTGGTCCGAGAGAACAACAT+GGG + chr2_4:49448138-49448157 Msa0332390:CDS 45.0%
GAGAGAACAACATGGGAAGA+AGG + chr2_4:49448145-49448164 Msa0332390:CDS 45.0%
GCTTGAAGAAAACCATGGAC+TGG + chr2_4:49448167-49448186 Msa0332390:CDS 45.0%
GGGATGGAGAGACATTTCAA+TGG + chr2_4:49448268-49448287 Msa0332390:CDS 45.0%
! GTTCCACCTACAAAGAGCAT+TGG + chr2_4:49448346-49448365 Msa0332390:CDS 45.0%
GAAGAGCCAATGCTCTTTGT+AGG - chr2_4:49448355-49448374 None:intergenic 45.0%
!! ATATGGAAAAGGGCGTCTTG+AGG + chr2_4:49448471-49448490 Msa0332390:CDS 45.0%
AAGTCCCAACAACGTTGACA+CGG - chr2_4:49448615-49448634 None:intergenic 45.0%
ATCCAGAAGGTTCAGGCATT+GGG + chr2_4:49448719-49448738 Msa0332390:CDS 45.0%
!! AGGTTCAGGCATTGGGTTTA+AGG + chr2_4:49448726-49448745 Msa0332390:CDS 45.0%
GAAGACACTCCTAATTTCGC+CGG + chr2_4:49448751-49448770 Msa0332390:CDS 45.0%
! GCTTACCATTGCCTTGGTTT+TGG - chr2_4:49448788-49448807 None:intergenic 45.0%
! TAGCTAGCTTACCATTGCCT+TGG - chr2_4:49448794-49448813 None:intergenic 45.0%
ATGGCAAAGAGGAACCTAAG+AGG + chr2_4:49449154-49449173 Msa0332390:CDS 45.0%
!! AGAGGAACCTAAGAGGTATC+TGG + chr2_4:49449161-49449180 Msa0332390:CDS 45.0%
ACATCTCGAGAATCTCGTTG+TGG - chr2_4:49449213-49449232 None:intergenic 45.0%
!! GTTACCTTGTGAGCTTGCTA+CGG + chr2_4:49449648-49449667 Msa0332390:three_prime_UTR 45.0%
TTAACCGTAGCAAGCTCACA+AGG - chr2_4:49449655-49449674 None:intergenic 45.0%
TTGTTCCCTCACTCTTGCTC+TGG + chr2_4:49446127-49446146 Msa0332390:intron 50.0%
TTGCGTCTCATGTTGCCAAC+CGG + chr2_4:49447288-49447307 Msa0332390:CDS 50.0%
! ATGTTGCCAACCGGCTTATC+CGG + chr2_4:49447297-49447316 Msa0332390:CDS 50.0%
ATAGTTCCGGATAAGCCGGT+TGG - chr2_4:49447306-49447325 None:intergenic 50.0%
GGTCTATGGAGAGACAGATG+AGG + chr2_4:49447637-49447656 Msa0332390:CDS 50.0%
TGGAGAGACAGATGAGGATG+CGG + chr2_4:49447643-49447662 Msa0332390:CDS 50.0%
ACAGATGAGGATGCGGTTGT+TGG + chr2_4:49447650-49447669 Msa0332390:CDS 50.0%
CCGTACTCTGCAACAAAAGC+TGG + chr2_4:49447707-49447726 Msa0332390:CDS 50.0%
TGGGGCTGAAATGCTTGTCA+TGG + chr2_4:49447727-49447746 Msa0332390:CDS 50.0%
TTGCCGGTAATCACAACACG+TGG + chr2_4:49447770-49447789 Msa0332390:CDS 50.0%
GTCCAACCTCTCCCTTATGA+AGG - chr2_4:49447952-49447971 None:intergenic 50.0%
GTTGGTCCGAGAGAACAACA+TGG + chr2_4:49448137-49448156 Msa0332390:CDS 50.0%
GTTAATGATGCCTGGTGGGA+TGG + chr2_4:49448252-49448271 Msa0332390:CDS 50.0%
AAATGTCTCTCCATCCCACC+AGG - chr2_4:49448265-49448284 None:intergenic 50.0%
GAGCCAATGCTCTTTGTAGG+TGG - chr2_4:49448352-49448371 None:intergenic 50.0%
!! GTTGGATCGGTGGTTTGCTT+GGG + chr2_4:49448416-49448435 Msa0332390:CDS 50.0%
TGAGGATCGTTCATCACTCG+TGG + chr2_4:49448489-49448508 Msa0332390:CDS 50.0%
TTTCAATGCAGCAGGCGTGA+CGG + chr2_4:49448543-49448562 Msa0332390:CDS 50.0%
CGGGAAGACCTAACGTTGAT+TGG + chr2_4:49448563-49448582 Msa0332390:CDS 50.0%
ACCATCCGTGTCAACGTTGT+TGG + chr2_4:49448607-49448626 Msa0332390:CDS 50.0%
CCATCCGTGTCAACGTTGTT+GGG + chr2_4:49448608-49448627 Msa0332390:CDS 50.0%
GAAGCTCATCCAGAAGGTTC+AGG + chr2_4:49448712-49448731 Msa0332390:CDS 50.0%
CATCCAGAAGGTTCAGGCAT+TGG + chr2_4:49448718-49448737 Msa0332390:CDS 50.0%
AACCCAATGCCTGAACCTTC+TGG - chr2_4:49448724-49448743 None:intergenic 50.0%
GAGAATCTCGTTGTGGCTCA+CGG - chr2_4:49449206-49449225 None:intergenic 50.0%
CGGGATAGTTCCGGATAAGC+CGG - chr2_4:49447310-49447329 None:intergenic 55.0%
TGCGGTTGTTGGAAACCACG+AGG + chr2_4:49447661-49447680 Msa0332390:CDS 55.0%
GAAGTAGCTGAGAAGCCTCG+TGG - chr2_4:49447679-49447698 None:intergenic 55.0%
CTCACATCGCCCCACCAATT+AGG - chr2_4:49448205-49448224 None:intergenic 55.0%
AATTGGTGGGGCGATGTGAG+TGG + chr2_4:49448205-49448224 Msa0332390:CDS 55.0%
GGAAGAACTGGTTGGATCGG+TGG + chr2_4:49448406-49448425 Msa0332390:CDS 55.0%
!! GGTTGGATCGGTGGTTTGCT+TGG + chr2_4:49448415-49448434 Msa0332390:CDS 55.0%
TTCAATGCAGCAGGCGTGAC+GGG + chr2_4:49448544-49448563 Msa0332390:CDS 55.0%
CCCAACAACGTTGACACGGA+TGG - chr2_4:49448611-49448630 None:intergenic 55.0%
ATCCTCATTACCGGGGCAGC+TGG + chr2_4:49447260-49447279 Msa0332390:CDS 60.0%
ATCCAGCTGCCCCGGTAATG+AGG - chr2_4:49447265-49447284 None:intergenic 60.0%
CGCAATGAATCCAGCTGCCC+CGG - chr2_4:49447273-49447292 None:intergenic 60.0%
TCGTTGTGGCTCACGGCGCC+AGG - chr2_4:49449199-49449218 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 49445995 49449831 49445995 ID=Msa0332390;Name=Msa0332390
chr2_4 mRNA 49445995 49449831 49445995 ID=Msa0332390-mRNA-1;Parent=Msa0332390;Name=Msa0332390-mRNA-1;_AED=0.02;_eAED=0.02;_QI=133|1|1|1|0.5|0.33|3|408|670
chr2_4 exon 49445995 49446116 49445995 ID=Msa0332390-mRNA-1:exon:14784;Parent=Msa0332390-mRNA-1
chr2_4 exon 49447225 49448801 49447225 ID=Msa0332390-mRNA-1:exon:14785;Parent=Msa0332390-mRNA-1
chr2_4 exon 49448977 49449831 49448977 ID=Msa0332390-mRNA-1:exon:14786;Parent=Msa0332390-mRNA-1
chr2_4 five_prime_UTR 49445995 49446116 49445995 ID=Msa0332390-mRNA-1:five_prime_utr;Parent=Msa0332390-mRNA-1
chr2_4 five_prime_UTR 49447225 49447235 49447225 ID=Msa0332390-mRNA-1:five_prime_utr;Parent=Msa0332390-mRNA-1
chr2_4 CDS 49447236 49448801 49447236 ID=Msa0332390-mRNA-1:cds;Parent=Msa0332390-mRNA-1
chr2_4 CDS 49448977 49449423 49448977 ID=Msa0332390-mRNA-1:cds;Parent=Msa0332390-mRNA-1
chr2_4 three_prime_UTR 49449424 49449831 49449424 ID=Msa0332390-mRNA-1:three_prime_utr;Parent=Msa0332390-mRNA-1
Gene Sequence

>Msa0332390

ATGGCAACGCACACCCCAAAAAACATCCTCATTACCGGGGCAGCTGGATTCATTGCGTCTCATGTTGCCAACCGGCTTATCCGGAACTATCCCGACTACAAAATTGTTGTCCTCGACAAACTCGATTACTGTTCTAATCTGAAGAACCTCATTCCTTCAAGATCATCTCCAAACTTCAAGTTTGTGAAGGGAGACATTGAAAGTGCTGACCTTGTCAACTACCTTCTCATCACTGAGTCTATCGACACAATTATGCATTTTGCTGCTCAAACTCATGTCGACAACTCTTTTGGTAACAGCTTTGAGTTCACCAAGAACAACATATATGGTACTCATGTCTTATTAGAAGCTTGCAAGGTAACTGGTCAGATCAAAAGATTCATCCATGTAAGCACTGATGAGGTCTATGGAGAGACAGATGAGGATGCGGTTGTTGGAAACCACGAGGCTTCTCAGCTACTTCCAACAAATCCGTACTCTGCAACAAAAGCTGGGGCTGAAATGCTTGTCATGGCGTATGGTAGATCTTATGGATTGCCGGTAATCACAACACGTGGAAACAATGTCTATGGCCCAAATCAGTTTCCTGAAAAGTTGATTCCCAAGTTTATCCTCTTGGCAATGCAAGGAAAAGTTCTTCCGATTCATGGTGATGGTTCAAATGTGAGGAGTTATCTGTATTGTGAAGATGTTGCGGAGGCTTTTGAAGTTATCCTTCATAAGGGAGAGGTTGGACATGTTTACAATATTGGAACTAAGAAGGAAAGGAGAGTTATTGATGTAGCCACTGATATATGCAAGCTCTTCTCAATTGATCCGGAGACTAGCATAAAATTTGTAGAGAACAGACCGTTTAATGATCAGAGATACTTTCTTGACGATCAAAAGTTGAAGGTTTTGGGTTGGTCCGAGAGAACAACATGGGAAGAAGGCTTGAAGAAAACCATGGACTGGTATATCAACAATCCTAATTGGTGGGGCGATGTGAGTGGTGCATTGCTTCCTCATCCGAAAATGTTAATGATGCCTGGTGGGATGGAGAGACATTTCAATGGGTCTGAAGAAGAAAATTCTGCACCGTTTGTCTCAAGTACTAATACTCGAATGGTGGTTCCACCTACAAAGAGCATTGGCTCTTCGCAGAAACCTCCTCTCAAGTTCTTGATCTATGGAAGAACTGGTTGGATCGGTGGTTTGCTTGGGAAATTGTGTGAGAAACAAGGGATTCCATATGAATATGGAAAAGGGCGTCTTGAGGATCGTTCATCACTCGTGGCTGATATTCAGAACGTGAAGCCAACTCATATTTTCAATGCAGCAGGCGTGACGGGAAGACCTAACGTTGATTGGTGTGAATCTCATAAAACTGAAACCATCCGTGTCAACGTTGTTGGGACTTTAACATTGGCTGATGTAAGCAGAGAGCATAACCTTTTGATGATGAACTATGCTACCGGGTGCATATTCGAGTATGATGAAGCTCATCCAGAAGGTTCAGGCATTGGGTTTAAGGAAGAAGACACTCCTAATTTCGCCGGTTCTTTCTATTCCAAAACCAAGGCAATGGTTGAAGAGCTTTTGAAAGACTATGACAATGTATGCACTCTCAGGGTTCGCATGCCAATTTCATCTGACCTAAGCAATCCACGCAATTTCATCACCAAGATTTCACGTTACAATAAAGTAGTCAACATTCCAAACAGCATGACTGTTTTGGACGAACTTCTTCCTATTTCAATTGAGATGGCAAAGAGGAACCTAAGAGGTATCTGGAACTTCACAAATCCTGGCGCCGTGAGCCACAACGAGATTCTCGAGATGTACAAAGACTACATTGATCCAAACTTCAAGTGGGCTAACTTCACACTTGAAGAACAAGCCAAAGTGATAGTTGCTGCAAGAAGCAACAATGAAATGGATGCATCCAAATTGAAGAATGAATTCCCAGAATTGCTTTCCATCAAAGAATCACTTATCAAGTACGTCTTTGAGCTAAACAAGAAAAGTGCTTAA
Protein sequence

>Msa0332390

MATHTPKNILITGAAGFIASHVANRLIRNYPDYKIVVLDKLDYCSNLKNLIPSRSSPNFKFVKGDIESADLVNYLLITESIDTIMHFAAQTHVDNSFGNSFEFTKNNIYGTHVLLEACKVTGQIKRFIHVSTDEVYGETDEDAVVGNHEASQLLPTNPYSATKAGAEMLVMAYGRSYGLPVITTRGNNVYGPNQFPEKLIPKFILLAMQGKVLPIHGDGSNVRSYLYCEDVAEAFEVILHKGEVGHVYNIGTKKERRVIDVATDICKLFSIDPETSIKFVENRPFNDQRYFLDDQKLKVLGWSERTTWEEGLKKTMDWYINNPNWWGDVSGALLPHPKMLMMPGGMERHFNGSEEENSAPFVSSTNTRMVVPPTKSIGSSQKPPLKFLIYGRTGWIGGLLGKLCEKQGIPYEYGKGRLEDRSSLVADIQNVKPTHIFNAAGVTGRPNVDWCESHKTETIRVNVVGTLTLADVSREHNLLMMNYATGCIFEYDEAHPEGSGIGFKEEDTPNFAGSFYSKTKAMVEELLKDYDNVCTLRVRMPISSDLSNPRNFITKISRYNKVVNIPNSMTVLDELLPISIEMAKRNLRGIWNFTNPGAVSHNEILEMYKDYIDPNFKWANFTLEEQAKVIVAARSNNEMDASKLKNEFPELLSIKESLIKYVFELNKKSA*