AlfalfaGEDB | Msa0332390

M. sativa cultivar zhongmu-4 / Msa0332390

KEGG: K12450
GO: GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006725 GO:0006793 GO:0006807 GO:0006810 GO:0008150 GO:0008152 GO:0009058 GO:0009225 GO:0009226 GO:0009506 GO:0009812 GO:0009813 GO:0009914 GO:0009966 GO:0009987 GO:0010253 GO:0010280 GO:0010315 GO:0010646 GO:0010817 GO:0010928 GO:0016829 GO:0016835 GO:0016836 GO:0018130 GO:0019438 GO:0023051 GO:0030054 GO:0030154 GO:0032502 GO:0033478 GO:0034641 GO:0034654 GO:0042127 GO:0042440 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046148 GO:0046483 GO:0048583 GO:0048869 GO:0050377 GO:0050789 GO:0050794 GO:0051179 GO:0051234 GO:0051552 GO:0051553 GO:0051554 GO:0051555 GO:0055044 GO:0055086 GO:0060918 GO:0065007 GO:0065008 GO:0071704 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617
PFAM: PF16363.8

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0332390	KEH38548.1	99.403	670	4	0	1	670	13	682	0.0	1390

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0332390	sp\|Q9SYM5\|RHM1_ARATH	88.077	671	76	2	1	669	1	669	0.0	1239

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0014170	Msa0332390	0.811865	5.653687e-51	-8.615850e-47
Msa0028340	Msa0332390	0.818568	1.831854e-52	-8.615850e-47
Msa0076170	Msa0332390	0.817482	3.223154e-52	-8.615850e-47
Msa0094710	Msa0332390	0.801739	7.811816e-49	-8.615850e-47
Msa0124200	Msa0332390	0.828032	1.125939e-54	-8.615850e-47
Msa0140880	Msa0332390	0.820850	5.516469e-53	-8.615850e-47
Msa0168570	Msa0332390	0.823427	1.393582e-53	-8.615850e-47
Msa0213880	Msa0332390	0.862921	3.598296e-64	-8.615850e-47
Msa0226310	Msa0332390	0.814920	1.204726e-51	-8.615850e-47
Msa0244890	Msa0332390	0.806748	7.074705e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0332390	MtrunA17_Chr2g0314601	99.403	670	4	0	1	670	1	670	0.0	1388
Msa0332390	MtrunA17_Chr8g0359401	89.521	668	69	1	1	668	1	667	0.0	1264
Msa0332390	MtrunA17_Chr1g0147841	74.925	666	155	2	4	669	2	655	0.0	1046
Msa0332390	MtrunA17_Chr4g0057161	82.007	289	52	0	380	668	7	295	2.14e-174	498
Msa0332390	MtrunA17_Chr3g0097081	61.780	191	42	2	85	274	1	161	9.56e-67	221
Msa0332390	MtrunA17_Chr3g0097081	52.893	121	50	1	362	475	137	257	5.86e-32	125
Msa0332390	MtrunA17_Chr2g0327981	30.573	314	189	10	9	312	40	334	5.86e-27	112
Msa0332390	MtrunA17_Chr4g0031131	28.617	311	199	10	9	312	34	328	4.16e-26	110
Msa0332390	MtrunA17_Chr4g0067461	27.619	315	195	11	7	312	111	401	1.96e-25	109
Msa0332390	MtrunA17_Chr8g0380921	26.243	362	218	15	1	327	63	410	9.46e-25	107

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0332390	AT1G78570.1	88.077	671	76	2	1	669	1	669	0.0	1239
Msa0332390	AT3G14790.2	85.351	669	93	2	1	669	4	667	0.0	1170
Msa0332390	AT3G14790.1	85.351	669	93	2	1	669	1	664	0.0	1170
Msa0332390	AT1G53500.1	85.479	668	93	3	2	669	4	667	0.0	1161
Msa0332390	AT1G63000.1	83.039	283	48	0	387	669	15	297	1.56e-177	507
Msa0332390	AT2G28760.1	30.032	313	192	10	9	312	33	327	4.25e-28	116
Msa0332390	AT2G28760.2	30.032	313	192	10	9	312	33	327	4.25e-28	116
Msa0332390	AT2G28760.3	30.032	313	192	10	9	312	33	327	4.25e-28	116
Msa0332390	AT4G20460.1	27.714	350	212	12	8	327	71	409	7.08e-28	117
Msa0332390	AT2G28760.4	30.032	313	192	10	9	312	64	358	1.02e-27	115

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Find 172 sgRNAs with CRISPR-Local

Find 236 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGTTAATGATGCCTGG+TGG	0.736377	2_4:+49448247	Msa0332390:CDS
AAATGTCTCTCCATCCCACC+AGG	0.569236	2_4:-49448262	None:intergenic
AAATGTTAATGATGCCTGGT+GGG	0.666092	2_4:+49448248	Msa0332390:CDS
AACAGTAATCGAGTTTGTCG+AGG	0.615310	2_4:-49447346	None:intergenic
AACCCAATGCCTGAACCTTC+TGG	0.294957	2_4:-49448721	None:intergenic
AAGAAGTGAAGAGAGAAAAT+AGG	0.287535	2_4:-49446075	None:intergenic
AAGTACTAATACTCGAATGG+TGG	0.665787	2_4:+49448324	Msa0332390:CDS
AAGTATCTCTGATCATTAAA+CGG	0.469948	2_4:-49448085	None:intergenic
AAGTCCCAACAACGTTGACA+CGG	0.632986	2_4:-49448612	None:intergenic
AAGTTAGCCCACTTGAAGTT+TGG	0.358387	2_4:-49449250	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATCTACGGAGATTTAGATT+TGG	-	chr2_4:49446043-49446062	None:intergenic	30.0%
AAGAAGTGAAGAGAGAAAAT+AGG	-	chr2_4:49446078-49446097	None:intergenic	30.0%
TTCTTTCATTGATCTTGTGA+TGG	+	chr2_4:49446166-49446185	Msa0332390:intron	30.0%
CAAAATCCAAATCATCAACT+GGG	-	chr2_4:49446192-49446211	None:intergenic	30.0%
ACAAAATCCAAATCATCAAC+TGG	-	chr2_4:49446193-49446212	None:intergenic	30.0%
GAATCTGATTCATGAAATTG+TGG	+	chr2_4:49446443-49446462	Msa0332390:intron	30.0%
AAAACTCACACAAGTTAAAG+TGG	-	chr2_4:49446671-49446690	None:intergenic	30.0%
TGGGATTCATCATAATGAAT+CGG	-	chr2_4:49446761-49446780	None:intergenic	30.0%
TCGAGTCATTACAATGAATT+GGG	-	chr2_4:49446780-49446799	None:intergenic	30.0%
ATCGAGTCATTACAATGAAT+TGG	-	chr2_4:49446781-49446800	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	49445995	49449831	49445995	ID=Msa0332390;Name=Msa0332390
chr2_4	mRNA	49445995	49449831	49445995	ID=Msa0332390-mRNA-1;Parent=Msa0332390;Name=Msa0332390-mRNA-1;_AED=0.02;_eAED=0.02;_QI=133\|1\|1\|1\|0.5\|0.33\|3\|408\|670
chr2_4	exon	49445995	49446116	49445995	ID=Msa0332390-mRNA-1:exon:14784;Parent=Msa0332390-mRNA-1
chr2_4	exon	49447225	49448801	49447225	ID=Msa0332390-mRNA-1:exon:14785;Parent=Msa0332390-mRNA-1
chr2_4	exon	49448977	49449831	49448977	ID=Msa0332390-mRNA-1:exon:14786;Parent=Msa0332390-mRNA-1
chr2_4	five_prime_UTR	49445995	49446116	49445995	ID=Msa0332390-mRNA-1:five_prime_utr;Parent=Msa0332390-mRNA-1
chr2_4	five_prime_UTR	49447225	49447235	49447225	ID=Msa0332390-mRNA-1:five_prime_utr;Parent=Msa0332390-mRNA-1
chr2_4	CDS	49447236	49448801	49447236	ID=Msa0332390-mRNA-1:cds;Parent=Msa0332390-mRNA-1
chr2_4	CDS	49448977	49449423	49448977	ID=Msa0332390-mRNA-1:cds;Parent=Msa0332390-mRNA-1
chr2_4	three_prime_UTR	49449424	49449831	49449424	ID=Msa0332390-mRNA-1:three_prime_utr;Parent=Msa0332390-mRNA-1

Gene Sequence

>Msa0332390

ATGGCAACGCACACCCCAAAAAACATCCTCATTACCGGGGCAGCTGGATTCATTGCGTCTCATGTTGCCAACCGGCTTATCCGGAACTATCCCGACTACAAAATTGTTGTCCTCGACAAACTCGATTACTGTTCTAATCTGAAGAACCTCATTCCTTCAAGATCATCTCCAAACTTCAAGTTTGTGAAGGGAGACATTGAAAGTGCTGACCTTGTCAACTACCTTCTCATCACTGAGTCTATCGACACAATTATGCATTTTGCTGCTCAAACTCATGTCGACAACTCTTTTGGTAACAGCTTTGAGTTCACCAAGAACAACATATATGGTACTCATGTCTTATTAGAAGCTTGCAAGGTAACTGGTCAGATCAAAAGATTCATCCATGTAAGCACTGATGAGGTCTATGGAGAGACAGATGAGGATGCGGTTGTTGGAAACCACGAGGCTTCTCAGCTACTTCCAACAAATCCGTACTCTGCAACAAAAGCTGGGGCTGAAATGCTTGTCATGGCGTATGGTAGATCTTATGGATTGCCGGTAATCACAACACGTGGAAACAATGTCTATGGCCCAAATCAGTTTCCTGAAAAGTTGATTCCCAAGTTTATCCTCTTGGCAATGCAAGGAAAAGTTCTTCCGATTCATGGTGATGGTTCAAATGTGAGGAGTTATCTGTATTGTGAAGATGTTGCGGAGGCTTTTGAAGTTATCCTTCATAAGGGAGAGGTTGGACATGTTTACAATATTGGAACTAAGAAGGAAAGGAGAGTTATTGATGTAGCCACTGATATATGCAAGCTCTTCTCAATTGATCCGGAGACTAGCATAAAATTTGTAGAGAACAGACCGTTTAATGATCAGAGATACTTTCTTGACGATCAAAAGTTGAAGGTTTTGGGTTGGTCCGAGAGAACAACATGGGAAGAAGGCTTGAAGAAAACCATGGACTGGTATATCAACAATCCTAATTGGTGGGGCGATGTGAGTGGTGCATTGCTTCCTCATCCGAAAATGTTAATGATGCCTGGTGGGATGGAGAGACATTTCAATGGGTCTGAAGAAGAAAATTCTGCACCGTTTGTCTCAAGTACTAATACTCGAATGGTGGTTCCACCTACAAAGAGCATTGGCTCTTCGCAGAAACCTCCTCTCAAGTTCTTGATCTATGGAAGAACTGGTTGGATCGGTGGTTTGCTTGGGAAATTGTGTGAGAAACAAGGGATTCCATATGAATATGGAAAAGGGCGTCTTGAGGATCGTTCATCACTCGTGGCTGATATTCAGAACGTGAAGCCAACTCATATTTTCAATGCAGCAGGCGTGACGGGAAGACCTAACGTTGATTGGTGTGAATCTCATAAAACTGAAACCATCCGTGTCAACGTTGTTGGGACTTTAACATTGGCTGATGTAAGCAGAGAGCATAACCTTTTGATGATGAACTATGCTACCGGGTGCATATTCGAGTATGATGAAGCTCATCCAGAAGGTTCAGGCATTGGGTTTAAGGAAGAAGACACTCCTAATTTCGCCGGTTCTTTCTATTCCAAAACCAAGGCAATGGTTGAAGAGCTTTTGAAAGACTATGACAATGTATGCACTCTCAGGGTTCGCATGCCAATTTCATCTGACCTAAGCAATCCACGCAATTTCATCACCAAGATTTCACGTTACAATAAAGTAGTCAACATTCCAAACAGCATGACTGTTTTGGACGAACTTCTTCCTATTTCAATTGAGATGGCAAAGAGGAACCTAAGAGGTATCTGGAACTTCACAAATCCTGGCGCCGTGAGCCACAACGAGATTCTCGAGATGTACAAAGACTACATTGATCCAAACTTCAAGTGGGCTAACTTCACACTTGAAGAACAAGCCAAAGTGATAGTTGCTGCAAGAAGCAACAATGAAATGGATGCATCCAAATTGAAGAATGAATTCCCAGAATTGCTTTCCATCAAAGAATCACTTATCAAGTACGTCTTTGAGCTAAACAAGAAAAGTGCTTAA

Protein sequence

>Msa0332390

MATHTPKNILITGAAGFIASHVANRLIRNYPDYKIVVLDKLDYCSNLKNLIPSRSSPNFKFVKGDIESADLVNYLLITESIDTIMHFAAQTHVDNSFGNSFEFTKNNIYGTHVLLEACKVTGQIKRFIHVSTDEVYGETDEDAVVGNHEASQLLPTNPYSATKAGAEMLVMAYGRSYGLPVITTRGNNVYGPNQFPEKLIPKFILLAMQGKVLPIHGDGSNVRSYLYCEDVAEAFEVILHKGEVGHVYNIGTKKERRVIDVATDICKLFSIDPETSIKFVENRPFNDQRYFLDDQKLKVLGWSERTTWEEGLKKTMDWYINNPNWWGDVSGALLPHPKMLMMPGGMERHFNGSEEENSAPFVSSTNTRMVVPPTKSIGSSQKPPLKFLIYGRTGWIGGLLGKLCEKQGIPYEYGKGRLEDRSSLVADIQNVKPTHIFNAAGVTGRPNVDWCESHKTETIRVNVVGTLTLADVSREHNLLMMNYATGCIFEYDEAHPEGSGIGFKEEDTPNFAGSFYSKTKAMVEELLKDYDNVCTLRVRMPISSDLSNPRNFITKISRYNKVVNIPNSMTVLDELLPISIEMAKRNLRGIWNFTNPGAVSHNEILEMYKDYIDPNFKWANFTLEEQAKVIVAARSNNEMDASKLKNEFPELLSIKESLIKYVFELNKKSA*