AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0254260

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0254260 MtrunA17_Chr2g0314601 99.403 670 4 0 1 670 1 670 0.0 1388
Msa0254260 MtrunA17_Chr8g0359401 89.521 668 69 1 1 668 1 667 0.0 1264
Msa0254260 MtrunA17_Chr1g0147841 74.925 666 155 2 4 669 2 655 0.0 1046
Msa0254260 MtrunA17_Chr4g0057161 82.007 289 52 0 380 668 7 295 2.14e-174 498
Msa0254260 MtrunA17_Chr3g0097081 61.780 191 42 2 85 274 1 161 9.56e-67 221
Msa0254260 MtrunA17_Chr3g0097081 52.893 121 50 1 362 475 137 257 5.86e-32 125
Msa0254260 MtrunA17_Chr2g0327981 30.573 314 189 10 9 312 40 334 5.86e-27 112
Msa0254260 MtrunA17_Chr4g0031131 28.617 311 199 10 9 312 34 328 4.16e-26 110
Msa0254260 MtrunA17_Chr4g0067461 27.619 315 195 11 7 312 111 401 1.96e-25 109
Msa0254260 MtrunA17_Chr8g0380921 26.243 362 218 15 1 327 63 410 9.46e-25 107
Msa0254260 MtrunA17_Chr2g0293521 25.978 358 224 13 1 327 63 410 2.94e-24 105
Msa0254260 MtrunA17_Chr4g0073351 27.193 342 208 9 8 321 96 424 3.07e-22 100
Msa0254260 MtrunA17_Chr4g0011281 26.822 343 208 8 8 321 102 430 2.09e-20 94.7
Msa0254260 MtrunA17_Chr1g0195521 26.524 328 201 13 8 312 119 429 1.01e-19 92.4
Msa0254260 MtrunA17_Chr4g0056941 28.274 336 212 10 7 320 93 421 1.75e-19 91.7
Msa0254260 MtrunA17_Chr5g0398811 26.571 350 222 10 5 326 88 430 2.98e-19 90.9
Msa0254260 MtrunA17_Chr7g0230291 26.608 342 210 8 8 321 83 411 4.06e-19 90.5
Msa0254260 MtrunA17_Chr8g0388261 27.066 351 217 13 7 328 4 344 1.47e-18 87.8
Msa0254260 MtrunA17_Chr4g0076751 24.217 351 225 14 7 327 25 364 1.96e-18 87.8
Msa0254260 MtrunA17_Chr3g0121661 26.647 334 216 10 9 320 101 427 3.25e-17 84.7
Msa0254260 MtrunA17_Chr3g0136511 29.796 245 147 9 1 234 1 231 1.09e-14 77.4
Msa0254260 MtrunA17_Chr1g0208111 24.377 361 218 14 6 318 18 371 1.26e-14 76.3
Msa0254260 MtrunA17_Chr1g0182091 28.372 215 140 7 105 312 4 211 2.63e-14 73.2
Msa0254260 MtrunA17_Chr3g0133771 24.280 243 169 6 7 248 2 230 1.63e-12 69.3
Msa0254260 MtrunA17_Chr4g0027491 23.546 361 221 13 6 318 18 371 2.74e-12 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0254260 AT1G78570.1 88.077 671 76 2 1 669 1 669 0.0 1239
Msa0254260 AT3G14790.2 85.351 669 93 2 1 669 4 667 0.0 1170
Msa0254260 AT3G14790.1 85.351 669 93 2 1 669 1 664 0.0 1170
Msa0254260 AT1G53500.1 85.479 668 93 3 2 669 4 667 0.0 1161
Msa0254260 AT1G63000.1 83.039 283 48 0 387 669 15 297 1.56e-177 507
Msa0254260 AT2G28760.1 30.032 313 192 10 9 312 33 327 4.25e-28 116
Msa0254260 AT2G28760.2 30.032 313 192 10 9 312 33 327 4.25e-28 116
Msa0254260 AT2G28760.3 30.032 313 192 10 9 312 33 327 4.25e-28 116
Msa0254260 AT4G20460.1 27.714 350 212 12 8 327 71 409 7.08e-28 117
Msa0254260 AT2G28760.4 30.032 313 192 10 9 312 64 358 1.02e-27 115
Msa0254260 AT5G59290.4 29.712 313 193 10 9 312 3 297 1.38e-27 114
Msa0254260 AT5G59290.1 29.712 313 193 10 9 312 32 326 1.94e-27 114
Msa0254260 AT5G59290.3 29.712 313 193 10 9 312 25 319 2.23e-27 114
Msa0254260 AT5G59290.2 29.712 313 193 10 9 312 47 341 3.14e-27 114
Msa0254260 AT3G46440.2 29.393 313 194 10 9 312 31 325 4.67e-27 113
Msa0254260 AT3G46440.1 29.393 313 194 10 9 312 31 325 4.67e-27 113
Msa0254260 AT3G53520.1 27.187 320 209 10 9 321 122 424 8.93e-25 108
Msa0254260 AT3G62830.2 27.157 313 200 10 9 312 121 414 1.32e-24 107
Msa0254260 AT3G62830.1 27.157 313 200 10 9 312 121 414 1.32e-24 107
Msa0254260 AT2G47650.1 26.837 313 201 10 9 312 123 416 5.34e-24 105
Msa0254260 AT5G44480.3 26.989 352 210 15 8 326 72 409 1.23e-23 104
Msa0254260 AT5G44480.4 26.989 352 210 15 8 326 72 409 1.23e-23 104
Msa0254260 AT5G44480.2 26.989 352 210 15 8 326 72 409 1.23e-23 104
Msa0254260 AT5G44480.1 26.989 352 210 15 8 326 96 433 1.46e-23 104
Msa0254260 AT2G47650.2 26.498 317 203 10 9 312 123 422 7.41e-23 102
Msa0254260 AT1G30620.3 27.809 356 214 15 4 327 65 409 1.37e-22 101
Msa0254260 AT3G53520.2 26.875 320 208 11 9 321 122 422 1.46e-22 101
Msa0254260 AT1G30620.1 27.809 356 214 15 4 327 66 410 1.48e-22 101
Msa0254260 AT1G30620.6 27.809 356 214 15 4 327 66 410 1.48e-22 101
Msa0254260 AT1G30620.4 27.809 356 214 15 4 327 66 410 1.48e-22 101
Msa0254260 AT1G30620.2 27.809 356 214 15 4 327 66 410 1.48e-22 101
Msa0254260 AT3G53520.4 25.948 343 207 11 9 321 122 447 8.46e-22 99.4
Msa0254260 AT1G63180.1 26.331 357 222 14 1 327 1 346 1.03e-20 94.7
Msa0254260 AT4G00110.1 26.844 339 219 8 8 324 92 423 2.23e-20 94.7
Msa0254260 AT2G45310.1 26.866 335 216 9 9 321 99 426 9.79e-20 92.8
Msa0254260 AT1G02000.1 25.893 336 220 9 8 321 93 421 7.98e-19 90.1
Msa0254260 AT4G30440.1 30.901 233 151 6 8 234 89 317 2.48e-18 88.6
Msa0254260 AT1G64440.1 27.920 351 210 15 8 327 4 342 5.59e-18 86.7
Msa0254260 AT1G30620.5 30.256 195 121 6 4 191 66 252 1.33e-17 84.3
Msa0254260 AT3G53520.3 27.897 233 148 7 8 234 121 339 2.25e-17 84.7
Msa0254260 AT4G23920.1 26.163 344 219 12 7 323 3 338 3.89e-16 80.9
Msa0254260 AT1G63180.2 26.689 296 181 11 1 267 1 289 4.45e-16 80.1
Msa0254260 AT3G23820.1 26.567 335 217 10 8 320 113 440 1.53e-15 80.1
Msa0254260 AT1G47290.1 26.757 370 201 15 3 319 6 358 6.57e-15 77.4
Msa0254260 AT4G12250.1 26.100 341 223 8 3 321 92 425 1.54e-14 77.0
Msa0254260 AT1G47290.2 26.757 370 201 15 3 319 6 358 1.76e-14 76.6
Msa0254260 AT2G26260.1 26.506 249 148 8 2 234 5 234 1.76e-13 73.9
Msa0254260 AT1G73250.1 27.473 182 120 5 151 322 140 319 8.06e-13 70.5

Find 173 sgRNAs with CRISPR-Local

Find 235 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGGAATTCATTCTTCAATT+TGG 0.161081 2_2:-47121086 None:intergenic
CGATCAAAAGTTGAAGGTTT+TGG 0.178673 2_2:+47119865 Msa0254260:CDS
AGGTTCAGGCATTGGGTTTA+AGG 0.187878 2_2:+47120477 Msa0254260:CDS
GCTTACCATTGCCTTGGTTT+TGG 0.211812 2_2:-47120536 None:intergenic
TCTTTGATGGAAAGCAATTC+TGG 0.218557 2_2:-47121106 None:intergenic
TAATTGATAAGTATTATAAT+TGG 0.230179 2_2:+47121310 Msa0254260:three_prime_UTR
GTTGGACATGTTTACAATAT+TGG 0.241068 2_2:+47119716 Msa0254260:CDS
CCCTTCACAAACTTGAAGTT+TGG 0.241790 2_2:-47119155 None:intergenic
TATTGCAGAAGTTGCTGATA+TGG 0.245883 2_2:+47118968 Msa0254260:intron
TTTGCCATCTCAATTGAAAT+AGG 0.250438 2_2:-47120890 None:intergenic
ATTGCCAAGAGGATAAATTT+TGG 0.258449 2_2:-47119587 None:intergenic
TAGAAAGAACCGGCGAAATT+AGG 0.282017 2_2:-47120511 None:intergenic
AAGAAGTGAAGAGAGAAAAT+AGG 0.287535 2_2:-47117827 None:intergenic
AACCCAATGCCTGAACCTTC+TGG 0.294957 2_2:-47120472 None:intergenic
CTTGATAAGTGATTCTTTGA+TGG 0.295568 2_2:-47121119 None:intergenic
CAAGGGATTCCATATGAATA+TGG 0.304468 2_2:+47120205 Msa0254260:CDS
AATTGATAAGTATTATAATT+GGG 0.306164 2_2:+47121311 Msa0254260:three_prime_UTR
TCCAAACAGCATGACTGTTT+TGG 0.309945 2_2:+47120856 Msa0254260:CDS
CCTCTCAAGTTCTTGATCTA+TGG 0.311827 2_2:+47120136 Msa0254260:CDS
GGTATATCAACAATCCTAAT+TGG 0.322317 2_2:+47119939 Msa0254260:CDS
ACTCATGTCGACAACTCTTT+TGG 0.326250 2_2:+47119257 Msa0254260:CDS
TGAAGTTCCAGATACCTCTT+AGG 0.329274 2_2:-47120919 None:intergenic
ATGGCGTATGGTAGGTCTTA+TGG 0.335557 2_2:+47119497 Msa0254260:CDS
TTGCTTAGGTCAGATGAAAT+TGG 0.338530 2_2:-47120782 None:intergenic
TTAGAAGCTTGCAAGGTAAC+TGG 0.349049 2_2:+47119329 Msa0254260:CDS
TTCTTCCCATGTTGTTCTCT+CGG 0.350950 2_2:-47119894 None:intergenic
TTTCCACGTGTTGTGATTAC+CGG 0.356238 2_2:-47119524 None:intergenic
AAGTTAGCCCACTTGAAGTT+TGG 0.358387 2_2:-47121001 None:intergenic
ATCCAGAAGGTTCAGGCATT+GGG 0.362912 2_2:+47120470 Msa0254260:CDS
TGTGAGTTATGTTTGTGTAT+TGG 0.363789 2_2:+47121484 Msa0254260:three_prime_UTR
CTCACATCGCCCCACCAATT+AGG 0.372048 2_2:-47119953 None:intergenic
AGTACCATATATGTTGTTCT+TGG 0.373878 2_2:-47119297 None:intergenic
ACACGTGGAAACAATGTCTA+TGG 0.379199 2_2:+47119536 Msa0254260:CDS
GGAAAAGTTCTTCCGATTCA+TGG 0.380568 2_2:+47119614 Msa0254260:CDS
TCCAAAACAGTCATGCTGTT+TGG 0.382722 2_2:-47120857 None:intergenic
TTTATTGTAACGTGAAATCT+TGG 0.382737 2_2:-47120822 None:intergenic
TTCTTGATCTATGGAAGAAC+TGG 0.390031 2_2:+47120145 Msa0254260:CDS
AGAGGAACCTAAGAGGTATC+TGG 0.395333 2_2:+47120912 Msa0254260:CDS
TTGTGTTTAGAAGATAATAT+TGG 0.401763 2_2:+47121287 Msa0254260:three_prime_UTR
CCGTACTCTGCAACAAAAGC+TGG 0.402137 2_2:+47119458 Msa0254260:CDS
TTCACCAAGAACAACATATA+TGG 0.402269 2_2:+47119293 Msa0254260:CDS
AATCTACGGAGATTTAGATT+TGG 0.404375 2_2:-47117792 None:intergenic
CTTTGATGGAAAGCAATTCT+GGG 0.407319 2_2:-47121105 None:intergenic
ATGTTGCCAACCGGCTTATC+CGG 0.407727 2_2:+47119048 Msa0254260:CDS
ATCCTCATTACCGGGGCAGC+TGG 0.408875 2_2:+47119011 Msa0254260:CDS
GGTTGGATCGGTGGTTTGCT+TGG 0.414855 2_2:+47120166 Msa0254260:CDS
GATTCCAAAATTTATCCTCT+TGG 0.417223 2_2:+47119583 Msa0254260:CDS
GTTGGATCGGTGGTTTGCTT+GGG 0.417488 2_2:+47120167 Msa0254260:CDS
ACAGATGAGGATGCGGTTGT+TGG 0.419126 2_2:+47119401 Msa0254260:CDS
TAAACACAACAATAACAGTA+TGG 0.420329 2_2:-47121273 None:intergenic
TCTTGACGATCAAAAGTTGA+AGG 0.421916 2_2:+47119859 Msa0254260:CDS
TAAGTATTATAATTGGGGTT+AGG 0.426960 2_2:+47121317 Msa0254260:three_prime_UTR
GTAAGCACTGATGAGGTCTA+TGG 0.433555 2_2:+47119374 Msa0254260:CDS
AAGTTTGGAGATGATCTTGA+AGG 0.433792 2_2:-47119140 None:intergenic
CGGGAAGACCTAACGTTGAT+TGG 0.437127 2_2:+47120314 Msa0254260:CDS
CATCCAGAAGGTTCAGGCAT+TGG 0.437868 2_2:+47120469 Msa0254260:CDS
GAAGAGCCAATGCTCTTTGT+AGG 0.440799 2_2:-47120103 None:intergenic
TTGCGTCTCATGTTGCCAAC+CGG 0.441564 2_2:+47119039 Msa0254260:CDS
TGGTAGGTCTTATGGATTGC+CGG 0.441733 2_2:+47119505 Msa0254260:CDS
ATGACAATGTATGCACTCTC+AGG 0.444077 2_2:+47120750 Msa0254260:CDS
ATTCCATATGAATATGGAAA+AGG 0.444160 2_2:+47120211 Msa0254260:CDS
ATTATAATTGGGGTTAGGTA+TGG 0.447743 2_2:+47121322 Msa0254260:three_prime_UTR
TAGCTAGCTTACCATTGCCT+TGG 0.452476 2_2:-47120542 None:intergenic
CGTTGTTGGGACTTTAACAT+TGG 0.453861 2_2:+47120372 Msa0254260:CDS
GAAGCTCATCCAGAAGGTTC+AGG 0.456647 2_2:+47120463 Msa0254260:CDS
TTCAATGCAGCAGGCGTGAC+GGG 0.457186 2_2:+47120295 Msa0254260:CDS
TTTGAAGTTATCCTTCATAA+GGG 0.460918 2_2:+47119689 Msa0254260:CDS
CCATCCGTGTCAACGTTGTT+GGG 0.465475 2_2:+47120359 Msa0254260:CDS
CGCAATGAATCCAGCTGCCC+CGG 0.466589 2_2:-47119021 None:intergenic
ACCATCCGTGTCAACGTTGT+TGG 0.468379 2_2:+47120358 Msa0254260:CDS
TCGTTGTGGCTCACGGCGCC+AGG 0.468437 2_2:-47120947 None:intergenic
AAGTATCTCTGATCATTAAA+CGG 0.469948 2_2:-47119836 None:intergenic
ATCTGGAACTTCACAAATCC+TGG 0.470303 2_2:+47120929 Msa0254260:CDS
TTACAATATTGGAACTAAGA+AGG 0.484035 2_2:+47119727 Msa0254260:CDS
CGTACTCTGCAACAAAAGCT+GGG 0.487722 2_2:+47119459 Msa0254260:CDS
GTTGCAGAGTACGGATTTGT+TGG 0.489137 2_2:-47119449 None:intergenic
GAGATGATCTTGAAGGAATG+AGG 0.489742 2_2:-47119133 None:intergenic
ATTGATAAGTATTATAATTG+GGG 0.495923 2_2:+47121312 Msa0254260:three_prime_UTR
ACTTACAAATTGGTACGAAA+AGG 0.496883 2_2:-47117852 None:intergenic
TCTTCCTATTTCAATTGAGA+TGG 0.498900 2_2:+47120886 Msa0254260:CDS
TCTTGCAGCAACTATCACTT+TGG 0.499126 2_2:-47121041 None:intergenic
ATCCTTCATAAGGGAGAGGT+TGG 0.502469 2_2:+47119698 Msa0254260:CDS
AGACCTCATCAGTGCTTACA+TGG 0.502767 2_2:-47119370 None:intergenic
TTCCATATGAATATGGAAAA+GGG 0.506901 2_2:+47120212 Msa0254260:CDS
CAAGCTCTTCTCAATTGATC+CGG 0.510856 2_2:+47119784 Msa0254260:CDS
TAGCATAGTTCATCATCAAA+AGG 0.516883 2_2:-47120417 None:intergenic
GTTCTTCCGATTCATGGTGA+TGG 0.517259 2_2:+47119620 Msa0254260:CDS
TGCAAGAAGCAACAATGAAA+TGG 0.518316 2_2:+47121057 Msa0254260:CDS
TGAAATTGCGTGGATTGCTT+AGG 0.528340 2_2:-47120796 None:intergenic
TCCAAACTTCAAGTTTGTGA+AGG 0.531089 2_2:+47119154 Msa0254260:CDS
TTTATCCTCTTGGCAATGCA+AGG 0.531302 2_2:+47119593 Msa0254260:CDS
TTGATGATGAACTATGCTAC+CGG 0.533136 2_2:+47120421 Msa0254260:CDS
GTTACCTTGTGAGCTTGCTA+CGG 0.534317 2_2:+47121399 Msa0254260:three_prime_UTR
GGGAAATTGTGTGAGAAACA+AGG 0.534485 2_2:+47120187 Msa0254260:CDS
TTTGAACCATCACCATGAAT+CGG 0.534712 2_2:-47119626 None:intergenic
TATCAACAATCCTAATTGGT+GGG 0.540325 2_2:+47119943 Msa0254260:CDS
GTCCAACCTCTCCCTTATGA+AGG 0.542052 2_2:-47119700 None:intergenic
TGATCTATGGAAGAACTGGT+TGG 0.542351 2_2:+47120149 Msa0254260:CDS
ATTGTTGATATACCAGTCCA+TGG 0.546234 2_2:-47119930 None:intergenic
GAGAGAACAACATGGGAAGA+AGG 0.546603 2_2:+47119896 Msa0254260:CDS
CCAAACTTCAAGTTTGTGAA+GGG 0.547634 2_2:+47119155 Msa0254260:CDS
GGAAGAACTGGTTGGATCGG+TGG 0.548201 2_2:+47120157 Msa0254260:CDS
CCGAAAATGTTAATGATGCC+TGG 0.549298 2_2:+47119995 Msa0254260:CDS
GTTAATGATGCCTGGTGGGA+TGG 0.550016 2_2:+47120003 Msa0254260:CDS
CGGGATAGTTCCGGATAAGC+CGG 0.551283 2_2:-47119058 None:intergenic
GATTCACACCAATCAACGTT+AGG 0.551310 2_2:-47120322 None:intergenic
GGAGTTGAAATCGAAATCTA+CGG 0.551446 2_2:-47117806 None:intergenic
ATCCAGCTGCCCCGGTAATG+AGG 0.552923 2_2:-47119013 None:intergenic
GAAATGCTCGTCATGGCGTA+TGG 0.556137 2_2:+47119485 Msa0254260:CDS
GGATGGAGAGACATTTCAAT+GGG 0.556466 2_2:+47120020 Msa0254260:CDS
TTCTTTCTATTCCAAAACCA+AGG 0.556902 2_2:+47120525 Msa0254260:CDS
TGCTCGTCATGGCGTATGGT+AGG 0.557344 2_2:+47119489 Msa0254260:CDS
CCCAACAACGTTGACACGGA+TGG 0.559674 2_2:-47120359 None:intergenic
TTTCAATGCAGCAGGCGTGA+CGG 0.562169 2_2:+47120294 Msa0254260:CDS
AGTTATCCTTCATAAGGGAG+AGG 0.562307 2_2:+47119694 Msa0254260:CDS
GGAAATTGTGTGAGAAACAA+GGG 0.564756 2_2:+47120188 Msa0254260:CDS
CGATAGACTCAGTGATGAGA+AGG 0.564861 2_2:-47119208 None:intergenic
CTATTCCAAAACCAAGGCAA+TGG 0.566752 2_2:+47120531 Msa0254260:CDS
AAATGTCTCTCCATCCCACC+AGG 0.569236 2_2:-47120013 None:intergenic
GGGATGGAGAGACATTTCAA+TGG 0.570949 2_2:+47120019 Msa0254260:CDS
TCATGTCTTCAATATCAGAA+TGG 0.573520 2_2:-47121431 None:intergenic
GAAGACACTCCTAATTTCGC+CGG 0.574480 2_2:+47120502 Msa0254260:CDS
ACATTGATCCAAACTTCAAG+TGG 0.579567 2_2:+47120993 Msa0254260:CDS
CTCAAGTACTAATACTCGAA+TGG 0.579731 2_2:+47120072 Msa0254260:CDS
TGTCTTATTAGAAGCTTGCA+AGG 0.580370 2_2:+47119322 Msa0254260:CDS
ATATCAACAATCCTAATTGG+TGG 0.585366 2_2:+47119942 Msa0254260:CDS
TGGAGAGACAGATGAGGATG+CGG 0.589535 2_2:+47119394 Msa0254260:CDS
TGGGGCTGAAATGCTCGTCA+TGG 0.592576 2_2:+47119478 Msa0254260:CDS
TATGGAAGAACTGGTTGGAT+CGG 0.593876 2_2:+47120154 Msa0254260:CDS
GAGCCAATGCTCTTTGTAGG+TGG 0.595767 2_2:-47120100 None:intergenic
GTTCCACCTACAAAGAGCAT+TGG 0.596551 2_2:+47120097 Msa0254260:CDS
TGAGGATCGTTCATCACTCG+TGG 0.597692 2_2:+47120240 Msa0254260:CDS
CCATAGATCAAGAACTTGAG+AGG 0.598084 2_2:-47120136 None:intergenic
ATCAACAATCCTAATTGGTG+GGG 0.599048 2_2:+47119944 Msa0254260:CDS
GCTTGAAGAAAACCATGGAC+TGG 0.603416 2_2:+47119918 Msa0254260:CDS
GTATTAGTACTTGAGACAAA+CGG 0.603642 2_2:-47120064 None:intergenic
GAGAATCTCGTTGTGGCTCA+CGG 0.604501 2_2:-47120954 None:intergenic
GCTGCATTGAAAATATGAGT+TGG 0.607206 2_2:-47120283 None:intergenic
GTTATCTAGCACTTATGCCG+TGG 0.611201 2_2:+47121561 Msa0254260:three_prime_UTR
ATCATACTCGAATATGCACC+CGG 0.611680 2_2:-47120440 None:intergenic
AACAGTAATCGAGTTTGTCG+AGG 0.615310 2_2:-47119097 None:intergenic
TGATGATGAACTATGCTACC+GGG 0.618950 2_2:+47120422 Msa0254260:CDS
ATGGCAAAGAGGAACCTAAG+AGG 0.622901 2_2:+47120905 Msa0254260:CDS
TATGATGAAGCTCATCCAGA+AGG 0.624291 2_2:+47120457 Msa0254260:CDS
ATATTGGAACTAAGAAGGAA+AGG 0.626669 2_2:+47119732 Msa0254260:CDS
TTTCAATTGAGATGGCAAAG+AGG 0.627252 2_2:+47120894 Msa0254260:CDS
TTTGTATTGTGAAGATGTTG+CGG 0.630976 2_2:+47119661 Msa0254260:CDS
CATCCATGTAAGCACTGATG+AGG 0.632531 2_2:+47119367 Msa0254260:CDS
AAGTCCCAACAACGTTGACA+CGG 0.632986 2_2:-47120363 None:intergenic
ATAGTTCCGGATAAGCCGGT+TGG 0.636641 2_2:-47119054 None:intergenic
AATTGGTGGGGCGATGTGAG+TGG 0.640742 2_2:+47119956 Msa0254260:CDS
ATCTTGGTGATGAAATTGCG+TGG 0.642464 2_2:-47120806 None:intergenic
ATATGGAAAAGGGCGTCTTG+AGG 0.643401 2_2:+47120222 Msa0254260:CDS
TGATGAGAAGGTAGTTGACA+AGG 0.649249 2_2:-47119196 None:intergenic
AGAAGGCTTGAAGAAAACCA+TGG 0.651189 2_2:+47119913 Msa0254260:CDS
TGACAATGTATGCACTCTCA+GGG 0.651558 2_2:+47120751 Msa0254260:CDS
TTAACCGTAGCAAGCTCACA+AGG 0.651715 2_2:-47121403 None:intergenic
GGTCTATGGAGAGACAGATG+AGG 0.654888 2_2:+47119388 Msa0254260:CDS
GAAGAGCTTGCATATATCAG+TGG 0.655262 2_2:-47119771 None:intergenic
CATTGATCCAAACTTCAAGT+GGG 0.662099 2_2:+47120994 Msa0254260:CDS
TTGCCGGTAATCACAACACG+TGG 0.662580 2_2:+47119521 Msa0254260:CDS
TAGATCAAGAACTTGAGAGG+AGG 0.663040 2_2:-47120133 None:intergenic
ACATCTCGAGAATCTCGTTG+TGG 0.664572 2_2:-47120961 None:intergenic
AAGTACTAATACTCGAATGG+TGG 0.665787 2_2:+47120075 Msa0254260:CDS
AAATGTTAATGATGCCTGGT+GGG 0.666092 2_2:+47119999 Msa0254260:CDS
GTACTCTGCAACAAAAGCTG+GGG 0.681769 2_2:+47119460 Msa0254260:CDS
TTGGTCCGAGAGAACAACAT+GGG 0.688575 2_2:+47119889 Msa0254260:CDS
GAAGTAGCTGAGAAGCCTCG+TGG 0.688718 2_2:-47119427 None:intergenic
ATGGTGATGGTTCAAATGTG+AGG 0.706627 2_2:+47119633 Msa0254260:CDS
GTTGGTCCGAGAGAACAACA+TGG 0.719943 2_2:+47119888 Msa0254260:CDS
TGCGGTTGTTGGAAACCACG+AGG 0.732893 2_2:+47119412 Msa0254260:CDS
AAAATGTTAATGATGCCTGG+TGG 0.736377 2_2:+47119998 Msa0254260:CDS
GTATTGTGAAGATGTTGCGG+AGG 0.741886 2_2:+47119664 Msa0254260:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTGATAAGTATTATAAT+TGG + chr2_2:47121310-47121329 Msa0254260:three_prime_UTR 10.0%
!! AATTGATAAGTATTATAATT+GGG + chr2_2:47121311-47121330 Msa0254260:three_prime_UTR 10.0%
!! GAAAAAAAAAACTTACAAAT+TGG - chr2_2:47117865-47117884 None:intergenic 15.0%
!! ATTCATAATCATAATGAAAA+TGG - chr2_2:47120674-47120693 None:intergenic 15.0%
!! ATTGATAAGTATTATAATTG+GGG + chr2_2:47121312-47121331 Msa0254260:three_prime_UTR 15.0%
!!! ACTAGATTTTTATTTAAATG+AGG - chr2_2:47121541-47121560 None:intergenic 15.0%
!!! TGTTTTAGTTGTTATTTTGA+AGG + chr2_2:47118118-47118137 Msa0254260:intron 20.0%
!!! TTGTTTTTACTGAATTTTAG+TGG + chr2_2:47118282-47118301 Msa0254260:intron 20.0%
!!! ATTGATATTTTGACTTGTTA+AGG + chr2_2:47118347-47118366 Msa0254260:intron 20.0%
!! ACATTCAATTTCTATACTAA+AGG - chr2_2:47118815-47118834 None:intergenic 20.0%
!! ACAGAAACTAAAATAAAACA+TGG - chr2_2:47118877-47118896 None:intergenic 20.0%
!!! TTTAGTTTCTGTGATTATTT+AGG + chr2_2:47118885-47118904 Msa0254260:intron 20.0%
!!! ATTTTGATTATATTTGTCTG+TGG + chr2_2:47118911-47118930 Msa0254260:intron 20.0%
!! AAAAAAAAAAGGAATCAGAA+TGG - chr2_2:47120596-47120615 None:intergenic 20.0%
!! TTGTGTTTAGAAGATAATAT+TGG + chr2_2:47121287-47121306 Msa0254260:three_prime_UTR 20.0%
!!! AATGAGTGTTTTTTATGATC+AGG + chr2_2:47117988-47118007 Msa0254260:intron 25.0%
!!! ATGAGTGTTTTTTATGATCA+GGG + chr2_2:47117989-47118008 Msa0254260:intron 25.0%
! TTTCATCATAAACAATCACA+TGG - chr2_2:47118077-47118096 None:intergenic 25.0%
!!! GATGTGTGTTTTTATTGTTT+AGG + chr2_2:47118214-47118233 Msa0254260:intron 25.0%
!!! TTTTACTGAATTTTAGTGGT+TGG + chr2_2:47118286-47118305 Msa0254260:intron 25.0%
!!! ATTTTGACTTGTTAAGGAAA+TGG + chr2_2:47118353-47118372 Msa0254260:intron 25.0%
!! TCAAGAAAATGTGTTACTAT+AGG + chr2_2:47118377-47118396 Msa0254260:intron 25.0%
! GATCTGTATCTTAAGATAAA+TGG - chr2_2:47118466-47118485 None:intergenic 25.0%
! ATAGTAAACACAGTAAGTTT+AGG - chr2_2:47118556-47118575 None:intergenic 25.0%
! ATAAGAAGTTAACAAGATCA+TGG - chr2_2:47118777-47118796 None:intergenic 25.0%
!!! ACTAAAGGTAAATTGACTTT+TGG - chr2_2:47118800-47118819 None:intergenic 25.0%
!!! AATTTTGGAATCAACTTTTC+AGG - chr2_2:47119575-47119594 None:intergenic 25.0%
!!! TTTTGAAGTTATCCTTCATA+AGG + chr2_2:47119688-47119707 Msa0254260:CDS 25.0%
!! TTTGAAGTTATCCTTCATAA+GGG + chr2_2:47119689-47119708 Msa0254260:CDS 25.0%
! TTACAATATTGGAACTAAGA+AGG + chr2_2:47119727-47119746 Msa0254260:CDS 25.0%
! AAGTATCTCTGATCATTAAA+CGG - chr2_2:47119839-47119858 None:intergenic 25.0%
! ATTCCATATGAATATGGAAA+AGG + chr2_2:47120211-47120230 Msa0254260:CDS 25.0%
! TTCCATATGAATATGGAAAA+GGG + chr2_2:47120212-47120231 Msa0254260:CDS 25.0%
! CCTCGATTTCAAAAAAAAAA+AGG - chr2_2:47120607-47120626 None:intergenic 25.0%
!!! CCTTTTTTTTTTTGAAATCG+AGG + chr2_2:47120604-47120623 Msa0254260:intron 25.0%
! TTTATTGTAACGTGAAATCT+TGG - chr2_2:47120825-47120844 None:intergenic 25.0%
! TAAACACAACAATAACAGTA+TGG - chr2_2:47121276-47121295 None:intergenic 25.0%
! TAAGTATTATAATTGGGGTT+AGG + chr2_2:47121317-47121336 Msa0254260:three_prime_UTR 25.0%
AATCTACGGAGATTTAGATT+TGG - chr2_2:47117795-47117814 None:intergenic 30.0%
AAGAAGTGAAGAGAGAAAAT+AGG - chr2_2:47117830-47117849 None:intergenic 30.0%
ACTTACAAATTGGTACGAAA+AGG - chr2_2:47117855-47117874 None:intergenic 30.0%
AAAAAAACAGAGAAAGAGTG+AGG - chr2_2:47117887-47117906 None:intergenic 30.0%
TTCTTTCATTGATCTTGTGA+TGG + chr2_2:47117915-47117934 Msa0254260:intron 30.0%
CAAAATCCAAATCATCAACT+GGG - chr2_2:47117941-47117960 None:intergenic 30.0%
ACAAAATCCAAATCATCAAC+TGG - chr2_2:47117942-47117961 None:intergenic 30.0%
GAATCTGATTCATGAAATTG+TGG + chr2_2:47118192-47118211 Msa0254260:intron 30.0%
AAAACTCACACAAGTTAAAG+TGG - chr2_2:47118422-47118441 None:intergenic 30.0%
TGGGATTCATCATAATGAAT+CGG - chr2_2:47118512-47118531 None:intergenic 30.0%
TCGAGTCATTACAATGAATT+GGG - chr2_2:47118531-47118550 None:intergenic 30.0%
ATCGAGTCATTACAATGAAT+TGG - chr2_2:47118532-47118551 None:intergenic 30.0%
TTCACCAAGAACAACATATA+TGG + chr2_2:47119293-47119312 Msa0254260:CDS 30.0%
AGTACCATATATGTTGTTCT+TGG - chr2_2:47119300-47119319 None:intergenic 30.0%
!! ACTTTTCAGGAAACTGATTT+GGG - chr2_2:47119562-47119581 None:intergenic 30.0%
!! AACTTTTCAGGAAACTGATT+TGG - chr2_2:47119563-47119582 None:intergenic 30.0%
GATTCCAAAATTTATCCTCT+TGG + chr2_2:47119583-47119602 Msa0254260:CDS 30.0%
! ATTGCCAAGAGGATAAATTT+TGG - chr2_2:47119590-47119609 None:intergenic 30.0%
TTTGTATTGTGAAGATGTTG+CGG + chr2_2:47119661-47119680 Msa0254260:CDS 30.0%
GTTGGACATGTTTACAATAT+TGG + chr2_2:47119716-47119735 Msa0254260:CDS 30.0%
ATATTGGAACTAAGAAGGAA+AGG + chr2_2:47119732-47119751 Msa0254260:CDS 30.0%
!! ACAAATTTTATGCTAGTCTC+CGG - chr2_2:47119806-47119825 None:intergenic 30.0%
! GATCAAAAGTTGAAGGTTTT+GGG + chr2_2:47119866-47119885 Msa0254260:CDS 30.0%
GGTATATCAACAATCCTAAT+TGG + chr2_2:47119939-47119958 Msa0254260:CDS 30.0%
ATATCAACAATCCTAATTGG+TGG + chr2_2:47119942-47119961 Msa0254260:CDS 30.0%
TATCAACAATCCTAATTGGT+GGG + chr2_2:47119943-47119962 Msa0254260:CDS 30.0%
! ATCATTAACATTTTCGGATG+AGG - chr2_2:47119992-47120011 None:intergenic 30.0%
GTATTAGTACTTGAGACAAA+CGG - chr2_2:47120067-47120086 None:intergenic 30.0%
TAGCATAGTTCATCATCAAA+AGG - chr2_2:47120420-47120439 None:intergenic 30.0%
TTCTTTCTATTCCAAAACCA+AGG + chr2_2:47120525-47120544 Msa0254260:CDS 30.0%
! TTGAACAAGATTTGTTCAAC+AGG - chr2_2:47120645-47120664 None:intergenic 30.0%
TTAATCTGTGAATGTTTCTC+AGG + chr2_2:47120706-47120725 Msa0254260:intron 30.0%
TCTTCCTATTTCAATTGAGA+TGG + chr2_2:47120886-47120905 Msa0254260:CDS 30.0%
TTTGCCATCTCAATTGAAAT+AGG - chr2_2:47120893-47120912 None:intergenic 30.0%
TGGGAATTCATTCTTCAATT+TGG - chr2_2:47121089-47121108 None:intergenic 30.0%
CTTGATAAGTGATTCTTTGA+TGG - chr2_2:47121122-47121141 None:intergenic 30.0%
! GCTACCAAATTTTTGTGTTT+TGG + chr2_2:47121212-47121231 Msa0254260:three_prime_UTR 30.0%
GTAGCCAAAACACAAAAATT+TGG - chr2_2:47121219-47121238 None:intergenic 30.0%
ATTATAATTGGGGTTAGGTA+TGG + chr2_2:47121322-47121341 Msa0254260:three_prime_UTR 30.0%
TCATGTCTTCAATATCAGAA+TGG - chr2_2:47121434-47121453 None:intergenic 30.0%
TGTGAGTTATGTTTGTGTAT+TGG + chr2_2:47121484-47121503 Msa0254260:three_prime_UTR 30.0%
GGAGTTGAAATCGAAATCTA+CGG - chr2_2:47117809-47117828 None:intergenic 35.0%
ATTGACTACACAAAGTTGAC+AGG - chr2_2:47118591-47118610 None:intergenic 35.0%
TATTGCAGAAGTTGCTGATA+TGG + chr2_2:47118968-47118987 Msa0254260:intron 35.0%
!!! GGTAATGAGGATGTTTTTTG+GGG - chr2_2:47119003-47119022 None:intergenic 35.0%
!!! CGGTAATGAGGATGTTTTTT+GGG - chr2_2:47119004-47119023 None:intergenic 35.0%
CCAAAAAACATCCTCATTAC+CGG + chr2_2:47119002-47119021 Msa0254260:CDS 35.0%
CAAAAAACATCCTCATTACC+GGG + chr2_2:47119003-47119022 Msa0254260:CDS 35.0%
AAAAAACATCCTCATTACCG+GGG + chr2_2:47119004-47119023 Msa0254260:CDS 35.0%
! AGGACAACAATTTTGTAGTC+GGG - chr2_2:47119080-47119099 None:intergenic 35.0%
! GAGGACAACAATTTTGTAGT+CGG - chr2_2:47119081-47119100 None:intergenic 35.0%
AAGTTTGGAGATGATCTTGA+AGG - chr2_2:47119143-47119162 None:intergenic 35.0%
! TCCAAACTTCAAGTTTGTGA+AGG + chr2_2:47119154-47119173 Msa0254260:CDS 35.0%
! CCAAACTTCAAGTTTGTGAA+GGG + chr2_2:47119155-47119174 Msa0254260:CDS 35.0%
! TGTCTTATTAGAAGCTTGCA+AGG + chr2_2:47119322-47119341 Msa0254260:CDS 35.0%
TTTGAACCATCACCATGAAT+CGG - chr2_2:47119629-47119648 None:intergenic 35.0%
TCTTGACGATCAAAAGTTGA+AGG + chr2_2:47119859-47119878 Msa0254260:CDS 35.0%
! CGATCAAAAGTTGAAGGTTT+TGG + chr2_2:47119865-47119884 Msa0254260:CDS 35.0%
!!! AAAAGTTGAAGGTTTTGGGT+TGG + chr2_2:47119870-47119889 Msa0254260:CDS 35.0%
ATTGTTGATATACCAGTCCA+TGG - chr2_2:47119933-47119952 None:intergenic 35.0%
ATCAACAATCCTAATTGGTG+GGG + chr2_2:47119944-47119963 Msa0254260:CDS 35.0%
! CCAGGCATCATTAACATTTT+CGG - chr2_2:47119998-47120017 None:intergenic 35.0%
AAAATGTTAATGATGCCTGG+TGG + chr2_2:47119998-47120017 Msa0254260:CDS 35.0%
AAATGTTAATGATGCCTGGT+GGG + chr2_2:47119999-47120018 Msa0254260:CDS 35.0%
CTCAAGTACTAATACTCGAA+TGG + chr2_2:47120072-47120091 Msa0254260:CDS 35.0%
AAGTACTAATACTCGAATGG+TGG + chr2_2:47120075-47120094 Msa0254260:CDS 35.0%
TTCTTGATCTATGGAAGAAC+TGG + chr2_2:47120145-47120164 Msa0254260:CDS 35.0%
GGAAATTGTGTGAGAAACAA+GGG + chr2_2:47120188-47120207 Msa0254260:CDS 35.0%
CAAGGGATTCCATATGAATA+TGG + chr2_2:47120205-47120224 Msa0254260:CDS 35.0%
GCTGCATTGAAAATATGAGT+TGG - chr2_2:47120286-47120305 None:intergenic 35.0%
! ACTCATATTTTCAATGCAGC+AGG + chr2_2:47120286-47120305 Msa0254260:CDS 35.0%
!! TTGATGATGAACTATGCTAC+CGG + chr2_2:47120421-47120440 Msa0254260:CDS 35.0%
!!! GGTTTTGGAATAGAAAGAAC+CGG - chr2_2:47120524-47120543 None:intergenic 35.0%
TTGCTTAGGTCAGATGAAAT+TGG - chr2_2:47120785-47120804 None:intergenic 35.0%
TTTCAATTGAGATGGCAAAG+AGG + chr2_2:47120894-47120913 Msa0254260:CDS 35.0%
ACATTGATCCAAACTTCAAG+TGG + chr2_2:47120993-47121012 Msa0254260:CDS 35.0%
CATTGATCCAAACTTCAAGT+GGG + chr2_2:47120994-47121013 Msa0254260:CDS 35.0%
TGCAAGAAGCAACAATGAAA+TGG + chr2_2:47121057-47121076 Msa0254260:CDS 35.0%
CTTTGATGGAAAGCAATTCT+GGG - chr2_2:47121108-47121127 None:intergenic 35.0%
TCTTTGATGGAAAGCAATTC+TGG - chr2_2:47121109-47121128 None:intergenic 35.0%
CACAAAGTTGACAGGAAAAG+TGG - chr2_2:47118583-47118602 None:intergenic 40.0%
!!! CCGGTAATGAGGATGTTTTT+TGG - chr2_2:47119005-47119024 None:intergenic 40.0%
! TTTTGTAGTCGGGATAGTTC+CGG - chr2_2:47119070-47119089 None:intergenic 40.0%
AACAGTAATCGAGTTTGTCG+AGG - chr2_2:47119100-47119119 None:intergenic 40.0%
GAGATGATCTTGAAGGAATG+AGG - chr2_2:47119136-47119155 None:intergenic 40.0%
CCCTTCACAAACTTGAAGTT+TGG - chr2_2:47119158-47119177 None:intergenic 40.0%
TGATGAGAAGGTAGTTGACA+AGG - chr2_2:47119199-47119218 None:intergenic 40.0%
! ACTCATGTCGACAACTCTTT+TGG + chr2_2:47119257-47119276 Msa0254260:CDS 40.0%
TTAGAAGCTTGCAAGGTAAC+TGG + chr2_2:47119329-47119348 Msa0254260:CDS 40.0%
TTTCCACGTGTTGTGATTAC+CGG - chr2_2:47119527-47119546 None:intergenic 40.0%
ACACGTGGAAACAATGTCTA+TGG + chr2_2:47119536-47119555 Msa0254260:CDS 40.0%
TTTATCCTCTTGGCAATGCA+AGG + chr2_2:47119593-47119612 Msa0254260:CDS 40.0%
GGAAAAGTTCTTCCGATTCA+TGG + chr2_2:47119614-47119633 Msa0254260:CDS 40.0%
!! ATGGTGATGGTTCAAATGTG+AGG + chr2_2:47119633-47119652 Msa0254260:CDS 40.0%
AGTTATCCTTCATAAGGGAG+AGG + chr2_2:47119694-47119713 Msa0254260:CDS 40.0%
GAAGAGCTTGCATATATCAG+TGG - chr2_2:47119774-47119793 None:intergenic 40.0%
CAAGCTCTTCTCAATTGATC+CGG + chr2_2:47119784-47119803 Msa0254260:CDS 40.0%
TTCTTCCCATGTTGTTCTCT+CGG - chr2_2:47119897-47119916 None:intergenic 40.0%
AGAAGGCTTGAAGAAAACCA+TGG + chr2_2:47119913-47119932 Msa0254260:CDS 40.0%
CCGAAAATGTTAATGATGCC+TGG + chr2_2:47119995-47120014 Msa0254260:CDS 40.0%
GGATGGAGAGACATTTCAAT+GGG + chr2_2:47120020-47120039 Msa0254260:CDS 40.0%
TAGATCAAGAACTTGAGAGG+AGG - chr2_2:47120136-47120155 None:intergenic 40.0%
CCATAGATCAAGAACTTGAG+AGG - chr2_2:47120139-47120158 None:intergenic 40.0%
CCTCTCAAGTTCTTGATCTA+TGG + chr2_2:47120136-47120155 Msa0254260:CDS 40.0%
TGATCTATGGAAGAACTGGT+TGG + chr2_2:47120149-47120168 Msa0254260:CDS 40.0%
TATGGAAGAACTGGTTGGAT+CGG + chr2_2:47120154-47120173 Msa0254260:CDS 40.0%
GGGAAATTGTGTGAGAAACA+AGG + chr2_2:47120187-47120206 Msa0254260:CDS 40.0%
! CGCCCTTTTCCATATTCATA+TGG - chr2_2:47120217-47120236 None:intergenic 40.0%
GATTCACACCAATCAACGTT+AGG - chr2_2:47120325-47120344 None:intergenic 40.0%
CGTTGTTGGGACTTTAACAT+TGG + chr2_2:47120372-47120391 Msa0254260:CDS 40.0%
! TGATGATGAACTATGCTACC+GGG + chr2_2:47120422-47120441 Msa0254260:CDS 40.0%
ATCATACTCGAATATGCACC+CGG - chr2_2:47120443-47120462 None:intergenic 40.0%
TATGATGAAGCTCATCCAGA+AGG + chr2_2:47120457-47120476 Msa0254260:CDS 40.0%
TAGAAAGAACCGGCGAAATT+AGG - chr2_2:47120514-47120533 None:intergenic 40.0%
CTATTCCAAAACCAAGGCAA+TGG + chr2_2:47120531-47120550 Msa0254260:CDS 40.0%
ATGACAATGTATGCACTCTC+AGG + chr2_2:47120750-47120769 Msa0254260:CDS 40.0%
TGACAATGTATGCACTCTCA+GGG + chr2_2:47120751-47120770 Msa0254260:CDS 40.0%
! TGAAATTGCGTGGATTGCTT+AGG - chr2_2:47120799-47120818 None:intergenic 40.0%
!! ATCTTGGTGATGAAATTGCG+TGG - chr2_2:47120809-47120828 None:intergenic 40.0%
! TCCAAACAGCATGACTGTTT+TGG + chr2_2:47120856-47120875 Msa0254260:CDS 40.0%
TCCAAAACAGTCATGCTGTT+TGG - chr2_2:47120860-47120879 None:intergenic 40.0%
TGAAGTTCCAGATACCTCTT+AGG - chr2_2:47120922-47120941 None:intergenic 40.0%
! ATCTGGAACTTCACAAATCC+TGG + chr2_2:47120929-47120948 Msa0254260:CDS 40.0%
AAGTTAGCCCACTTGAAGTT+TGG - chr2_2:47121004-47121023 None:intergenic 40.0%
TCTTGCAGCAACTATCACTT+TGG - chr2_2:47121044-47121063 None:intergenic 40.0%
!! TGATGGCCCAGTTGATGATT+TGG + chr2_2:47117932-47117951 Msa0254260:intron 45.0%
CGATAGACTCAGTGATGAGA+AGG - chr2_2:47119211-47119230 None:intergenic 45.0%
!! CATCCATGTAAGCACTGATG+AGG + chr2_2:47119367-47119386 Msa0254260:CDS 45.0%
AGACCTCATCAGTGCTTACA+TGG - chr2_2:47119373-47119392 None:intergenic 45.0%
!! GTAAGCACTGATGAGGTCTA+TGG + chr2_2:47119374-47119393 Msa0254260:CDS 45.0%
GTTGCAGAGTACGGATTTGT+TGG - chr2_2:47119452-47119471 None:intergenic 45.0%
! CCAGCTTTTGTTGCAGAGTA+CGG - chr2_2:47119461-47119480 None:intergenic 45.0%
CGTACTCTGCAACAAAAGCT+GGG + chr2_2:47119459-47119478 Msa0254260:CDS 45.0%
GTACTCTGCAACAAAAGCTG+GGG + chr2_2:47119460-47119479 Msa0254260:CDS 45.0%
ATGGCGTATGGTAGGTCTTA+TGG + chr2_2:47119497-47119516 Msa0254260:CDS 45.0%
! TGGTAGGTCTTATGGATTGC+CGG + chr2_2:47119505-47119524 Msa0254260:CDS 45.0%
! CTTTTCCTTGCATTGCCAAG+AGG - chr2_2:47119601-47119620 None:intergenic 45.0%
GTTCTTCCGATTCATGGTGA+TGG + chr2_2:47119620-47119639 Msa0254260:CDS 45.0%
GTATTGTGAAGATGTTGCGG+AGG + chr2_2:47119664-47119683 Msa0254260:CDS 45.0%
ATCCTTCATAAGGGAGAGGT+TGG + chr2_2:47119698-47119717 Msa0254260:CDS 45.0%
TTGGTCCGAGAGAACAACAT+GGG + chr2_2:47119889-47119908 Msa0254260:CDS 45.0%
GAGAGAACAACATGGGAAGA+AGG + chr2_2:47119896-47119915 Msa0254260:CDS 45.0%
GCTTGAAGAAAACCATGGAC+TGG + chr2_2:47119918-47119937 Msa0254260:CDS 45.0%
GGGATGGAGAGACATTTCAA+TGG + chr2_2:47120019-47120038 Msa0254260:CDS 45.0%
! GTTCCACCTACAAAGAGCAT+TGG + chr2_2:47120097-47120116 Msa0254260:CDS 45.0%
GAAGAGCCAATGCTCTTTGT+AGG - chr2_2:47120106-47120125 None:intergenic 45.0%
!! ATATGGAAAAGGGCGTCTTG+AGG + chr2_2:47120222-47120241 Msa0254260:CDS 45.0%
AAGTCCCAACAACGTTGACA+CGG - chr2_2:47120366-47120385 None:intergenic 45.0%
ATCCAGAAGGTTCAGGCATT+GGG + chr2_2:47120470-47120489 Msa0254260:CDS 45.0%
!! AGGTTCAGGCATTGGGTTTA+AGG + chr2_2:47120477-47120496 Msa0254260:CDS 45.0%
GAAGACACTCCTAATTTCGC+CGG + chr2_2:47120502-47120521 Msa0254260:CDS 45.0%
! GCTTACCATTGCCTTGGTTT+TGG - chr2_2:47120539-47120558 None:intergenic 45.0%
! TAGCTAGCTTACCATTGCCT+TGG - chr2_2:47120545-47120564 None:intergenic 45.0%
ATGGCAAAGAGGAACCTAAG+AGG + chr2_2:47120905-47120924 Msa0254260:CDS 45.0%
!! AGAGGAACCTAAGAGGTATC+TGG + chr2_2:47120912-47120931 Msa0254260:CDS 45.0%
ACATCTCGAGAATCTCGTTG+TGG - chr2_2:47120964-47120983 None:intergenic 45.0%
!! GTTACCTTGTGAGCTTGCTA+CGG + chr2_2:47121399-47121418 Msa0254260:three_prime_UTR 45.0%
TTAACCGTAGCAAGCTCACA+AGG - chr2_2:47121406-47121425 None:intergenic 45.0%
TTGCGTCTCATGTTGCCAAC+CGG + chr2_2:47119039-47119058 Msa0254260:CDS 50.0%
! ATGTTGCCAACCGGCTTATC+CGG + chr2_2:47119048-47119067 Msa0254260:CDS 50.0%
ATAGTTCCGGATAAGCCGGT+TGG - chr2_2:47119057-47119076 None:intergenic 50.0%
GGTCTATGGAGAGACAGATG+AGG + chr2_2:47119388-47119407 Msa0254260:CDS 50.0%
TGGAGAGACAGATGAGGATG+CGG + chr2_2:47119394-47119413 Msa0254260:CDS 50.0%
ACAGATGAGGATGCGGTTGT+TGG + chr2_2:47119401-47119420 Msa0254260:CDS 50.0%
CCGTACTCTGCAACAAAAGC+TGG + chr2_2:47119458-47119477 Msa0254260:CDS 50.0%
GAAATGCTCGTCATGGCGTA+TGG + chr2_2:47119485-47119504 Msa0254260:CDS 50.0%
TTGCCGGTAATCACAACACG+TGG + chr2_2:47119521-47119540 Msa0254260:CDS 50.0%
GTCCAACCTCTCCCTTATGA+AGG - chr2_2:47119703-47119722 None:intergenic 50.0%
GTTGGTCCGAGAGAACAACA+TGG + chr2_2:47119888-47119907 Msa0254260:CDS 50.0%
GTTAATGATGCCTGGTGGGA+TGG + chr2_2:47120003-47120022 Msa0254260:CDS 50.0%
AAATGTCTCTCCATCCCACC+AGG - chr2_2:47120016-47120035 None:intergenic 50.0%
GAGCCAATGCTCTTTGTAGG+TGG - chr2_2:47120103-47120122 None:intergenic 50.0%
!! GTTGGATCGGTGGTTTGCTT+GGG + chr2_2:47120167-47120186 Msa0254260:CDS 50.0%
TGAGGATCGTTCATCACTCG+TGG + chr2_2:47120240-47120259 Msa0254260:CDS 50.0%
TTTCAATGCAGCAGGCGTGA+CGG + chr2_2:47120294-47120313 Msa0254260:CDS 50.0%
CGGGAAGACCTAACGTTGAT+TGG + chr2_2:47120314-47120333 Msa0254260:CDS 50.0%
ACCATCCGTGTCAACGTTGT+TGG + chr2_2:47120358-47120377 Msa0254260:CDS 50.0%
CCATCCGTGTCAACGTTGTT+GGG + chr2_2:47120359-47120378 Msa0254260:CDS 50.0%
GAAGCTCATCCAGAAGGTTC+AGG + chr2_2:47120463-47120482 Msa0254260:CDS 50.0%
CATCCAGAAGGTTCAGGCAT+TGG + chr2_2:47120469-47120488 Msa0254260:CDS 50.0%
AACCCAATGCCTGAACCTTC+TGG - chr2_2:47120475-47120494 None:intergenic 50.0%
GAGAATCTCGTTGTGGCTCA+CGG - chr2_2:47120957-47120976 None:intergenic 50.0%
CGGGATAGTTCCGGATAAGC+CGG - chr2_2:47119061-47119080 None:intergenic 55.0%
TGCGGTTGTTGGAAACCACG+AGG + chr2_2:47119412-47119431 Msa0254260:CDS 55.0%
GAAGTAGCTGAGAAGCCTCG+TGG - chr2_2:47119430-47119449 None:intergenic 55.0%
TGGGGCTGAAATGCTCGTCA+TGG + chr2_2:47119478-47119497 Msa0254260:CDS 55.0%
TGCTCGTCATGGCGTATGGT+AGG + chr2_2:47119489-47119508 Msa0254260:CDS 55.0%
CTCACATCGCCCCACCAATT+AGG - chr2_2:47119956-47119975 None:intergenic 55.0%
AATTGGTGGGGCGATGTGAG+TGG + chr2_2:47119956-47119975 Msa0254260:CDS 55.0%
GGAAGAACTGGTTGGATCGG+TGG + chr2_2:47120157-47120176 Msa0254260:CDS 55.0%
!! GGTTGGATCGGTGGTTTGCT+TGG + chr2_2:47120166-47120185 Msa0254260:CDS 55.0%
TTCAATGCAGCAGGCGTGAC+GGG + chr2_2:47120295-47120314 Msa0254260:CDS 55.0%
CCCAACAACGTTGACACGGA+TGG - chr2_2:47120362-47120381 None:intergenic 55.0%
ATCCTCATTACCGGGGCAGC+TGG + chr2_2:47119011-47119030 Msa0254260:CDS 60.0%
ATCCAGCTGCCCCGGTAATG+AGG - chr2_2:47119016-47119035 None:intergenic 60.0%
CGCAATGAATCCAGCTGCCC+CGG - chr2_2:47119024-47119043 None:intergenic 60.0%
TCGTTGTGGCTCACGGCGCC+AGG - chr2_2:47120950-47120969 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 47117747 47121582 47117747 ID=Msa0254260;Name=Msa0254260
chr2_2 mRNA 47117747 47121582 47117747 ID=Msa0254260-mRNA-1;Parent=Msa0254260;Name=Msa0254260-mRNA-1;_AED=0.02;_eAED=0.02;_QI=133|1|1|1|0.5|0.33|3|408|670
chr2_2 exon 47117747 47117868 47117747 ID=Msa0254260-mRNA-1:exon:14360;Parent=Msa0254260-mRNA-1
chr2_2 exon 47118976 47120552 47118976 ID=Msa0254260-mRNA-1:exon:14361;Parent=Msa0254260-mRNA-1
chr2_2 exon 47120728 47121582 47120728 ID=Msa0254260-mRNA-1:exon:14362;Parent=Msa0254260-mRNA-1
chr2_2 five_prime_UTR 47117747 47117868 47117747 ID=Msa0254260-mRNA-1:five_prime_utr;Parent=Msa0254260-mRNA-1
chr2_2 five_prime_UTR 47118976 47118986 47118976 ID=Msa0254260-mRNA-1:five_prime_utr;Parent=Msa0254260-mRNA-1
chr2_2 CDS 47118987 47120552 47118987 ID=Msa0254260-mRNA-1:cds;Parent=Msa0254260-mRNA-1
chr2_2 CDS 47120728 47121174 47120728 ID=Msa0254260-mRNA-1:cds;Parent=Msa0254260-mRNA-1
chr2_2 three_prime_UTR 47121175 47121582 47121175 ID=Msa0254260-mRNA-1:three_prime_utr;Parent=Msa0254260-mRNA-1
Gene Sequence

>Msa0254260

ATGGCAACGCACACCCCAAAAAACATCCTCATTACCGGGGCAGCTGGATTCATTGCGTCTCATGTTGCCAACCGGCTTATCCGGAACTATCCCGACTACAAAATTGTTGTCCTCGACAAACTCGATTACTGTTCTAATCTGAAGAACCTCATTCCTTCAAGATCATCTCCAAACTTCAAGTTTGTGAAGGGAGACATTGAAAGTGCTGACCTTGTCAACTACCTTCTCATCACTGAGTCTATCGACACAATTATGCATTTTGCTGCTCAAACTCATGTCGACAACTCTTTTGGTAACAGCTTTGAGTTCACCAAGAACAACATATATGGTACTCATGTCTTATTAGAAGCTTGCAAGGTAACTGGTCAGATCAAAAGATTCATCCATGTAAGCACTGATGAGGTCTATGGAGAGACAGATGAGGATGCGGTTGTTGGAAACCACGAGGCTTCTCAGCTACTTCCAACAAATCCGTACTCTGCAACAAAAGCTGGGGCTGAAATGCTCGTCATGGCGTATGGTAGGTCTTATGGATTGCCGGTAATCACAACACGTGGAAACAATGTCTATGGCCCAAATCAGTTTCCTGAAAAGTTGATTCCAAAATTTATCCTCTTGGCAATGCAAGGAAAAGTTCTTCCGATTCATGGTGATGGTTCAAATGTGAGGAGTTATTTGTATTGTGAAGATGTTGCGGAGGCTTTTGAAGTTATCCTTCATAAGGGAGAGGTTGGACATGTTTACAATATTGGAACTAAGAAGGAAAGGAGAGTTATTGATGTAGCCACTGATATATGCAAGCTCTTCTCAATTGATCCGGAGACTAGCATAAAATTTGTAGAGAACAGACCGTTTAATGATCAGAGATACTTTCTTGACGATCAAAAGTTGAAGGTTTTGGGTTGGTCCGAGAGAACAACATGGGAAGAAGGCTTGAAGAAAACCATGGACTGGTATATCAACAATCCTAATTGGTGGGGCGATGTGAGTGGTGCATTGCTTCCTCATCCGAAAATGTTAATGATGCCTGGTGGGATGGAGAGACATTTCAATGGGTCTGAAGAAGAAAATTCTGCACCGTTTGTCTCAAGTACTAATACTCGAATGGTGGTTCCACCTACAAAGAGCATTGGCTCTTCGCAGAAACCTCCTCTCAAGTTCTTGATCTATGGAAGAACTGGTTGGATCGGTGGTTTGCTTGGGAAATTGTGTGAGAAACAAGGGATTCCATATGAATATGGAAAAGGGCGTCTTGAGGATCGTTCATCACTCGTGGCTGATATTCAGAACGTGAAGCCAACTCATATTTTCAATGCAGCAGGCGTGACGGGAAGACCTAACGTTGATTGGTGTGAATCTCATAAAACTGAAACCATCCGTGTCAACGTTGTTGGGACTTTAACATTGGCTGATGTAAGCAGAGAGCATAACCTTTTGATGATGAACTATGCTACCGGGTGCATATTCGAGTATGATGAAGCTCATCCAGAAGGTTCAGGCATTGGGTTTAAGGAAGAAGACACTCCTAATTTCGCCGGTTCTTTCTATTCCAAAACCAAGGCAATGGTTGAAGAGCTTTTGAAAGACTATGACAATGTATGCACTCTCAGGGTTCGCATGCCAATTTCATCTGACCTAAGCAATCCACGCAATTTCATCACCAAGATTTCACGTTACAATAAAGTAGTCAACATTCCAAACAGCATGACTGTTTTGGACGAACTTCTTCCTATTTCAATTGAGATGGCAAAGAGGAACCTAAGAGGTATCTGGAACTTCACAAATCCTGGCGCCGTGAGCCACAACGAGATTCTCGAGATGTACAAAGACTACATTGATCCAAACTTCAAGTGGGCTAACTTCACACTTGAAGAACAAGCCAAAGTGATAGTTGCTGCAAGAAGCAACAATGAAATGGATGCATCCAAATTGAAGAATGAATTCCCAGAATTGCTTTCCATCAAAGAATCACTTATCAAGTACGTCTTTGAGCTAAACAAGAAAAGTGCTTAA
Protein sequence

>Msa0254260

MATHTPKNILITGAAGFIASHVANRLIRNYPDYKIVVLDKLDYCSNLKNLIPSRSSPNFKFVKGDIESADLVNYLLITESIDTIMHFAAQTHVDNSFGNSFEFTKNNIYGTHVLLEACKVTGQIKRFIHVSTDEVYGETDEDAVVGNHEASQLLPTNPYSATKAGAEMLVMAYGRSYGLPVITTRGNNVYGPNQFPEKLIPKFILLAMQGKVLPIHGDGSNVRSYLYCEDVAEAFEVILHKGEVGHVYNIGTKKERRVIDVATDICKLFSIDPETSIKFVENRPFNDQRYFLDDQKLKVLGWSERTTWEEGLKKTMDWYINNPNWWGDVSGALLPHPKMLMMPGGMERHFNGSEEENSAPFVSSTNTRMVVPPTKSIGSSQKPPLKFLIYGRTGWIGGLLGKLCEKQGIPYEYGKGRLEDRSSLVADIQNVKPTHIFNAAGVTGRPNVDWCESHKTETIRVNVVGTLTLADVSREHNLLMMNYATGCIFEYDEAHPEGSGIGFKEEDTPNFAGSFYSKTKAMVEELLKDYDNVCTLRVRMPISSDLSNPRNFITKISRYNKVVNIPNSMTVLDELLPISIEMAKRNLRGIWNFTNPGAVSHNEILEMYKDYIDPNFKWANFTLEEQAKVIVAARSNNEMDASKLKNEFPELLSIKESLIKYVFELNKKSA*