Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0003890 | Msa0516720 | 0.805098 | 1.572361e-49 | -8.615850e-47 |
| Msa0007770 | Msa0516720 | 0.804258 | 2.354660e-49 | -8.615850e-47 |
| Msa0017510 | Msa0516720 | 0.807191 | 5.702846e-50 | -8.615850e-47 |
| Msa0040450 | Msa0516720 | 0.812105 | 5.012665e-51 | -8.615850e-47 |
| Msa0045790 | Msa0516720 | 0.801471 | 8.864126e-49 | -8.615850e-47 |
| Msa0046160 | Msa0516720 | 0.803985 | 2.684076e-49 | -8.615850e-47 |
| Msa0116770 | Msa0516720 | 0.800657 | 1.300218e-48 | -8.615850e-47 |
| Msa0136070 | Msa0516720 | 0.805757 | 1.144172e-49 | -8.615850e-47 |
| Msa0157290 | Msa0516720 | 0.806950 | 6.411457e-50 | -8.615850e-47 |
| Msa0165210 | Msa0516720 | 0.803599 | 3.227373e-49 | -8.615850e-47 |
| Msa0165220 | Msa0516720 | 0.805022 | 1.631110e-49 | -8.615850e-47 |
| Msa0197660 | Msa0516720 | 0.857252 | 1.852347e-62 | -8.615850e-47 |
| Msa0212310 | Msa0516720 | 0.800258 | 1.568296e-48 | -8.615850e-47 |
| Msa0230100 | Msa0516720 | 0.802161 | 6.397828e-49 | -8.615850e-47 |
| Msa0428080 | Msa0516720 | 0.820393 | 7.024710e-53 | -8.615850e-47 |
| Msa0239800 | Msa0516720 | 0.855210 | 7.350846e-62 | -8.615850e-47 |
| Msa0277820 | Msa0516720 | 0.835542 | 1.579743e-56 | -8.615850e-47 |
| Msa0317510 | Msa0516720 | 0.854765 | 9.898280e-62 | -8.615850e-47 |
| Msa0516720 | Msa0623080 | 0.817945 | 2.534660e-52 | -8.615850e-47 |
| Msa0516720 | Msa0657970 | 0.804110 | 2.527880e-49 | -8.615850e-47 |
| Msa0516720 | Msa0696450 | 0.806385 | 8.440745e-50 | -8.615850e-47 |
| Msa0516720 | Msa0880140 | 0.803529 | 3.337089e-49 | -8.615850e-47 |
| Msa0516720 | Msa0880950 | 0.806219 | 9.148192e-50 | -8.615850e-47 |
| Msa0516720 | Msa1014540 | 0.815155 | 1.068448e-51 | -8.615850e-47 |
| Msa0516720 | Msa1082500 | 0.805630 | 1.216489e-49 | -8.615850e-47 |
| Msa0516720 | Msa1238930 | 0.808220 | 3.446215e-50 | -8.615850e-47 |
| Msa0516720 | Msa1244250 | 0.807531 | 4.830669e-50 | -8.615850e-47 |
| Msa0516720 | Msa1249490 | 0.809722 | 1.644197e-50 | -8.615850e-47 |
| Msa0516720 | Msa1314170 | 0.819084 | 1.398429e-52 | -8.615850e-47 |
| Msa0516720 | Msa1409700 | 0.808239 | 3.415216e-50 | -8.615850e-47 |
| Msa0516720 | Msa1424750 | 0.813344 | 2.684346e-51 | -8.615850e-47 |
| Msa0516720 | Msa1436480 | 0.801739 | 7.811536e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTTACAAGAGGGTCAATT+TGG | 0.192572 | 3_4:+68225811 | Msa0516720:CDS |
| TTTGGAGACAAAATTATATA+TGG | 0.209786 | 3_4:+68225263 | Msa0516720:five_prime_UTR |
| GACAAGAATAAGGATGAATT+TGG | 0.329771 | 3_4:+68225245 | Msa0516720:five_prime_UTR |
| GGGTCAATTTGGGACTAAAT+AGG | 0.371012 | 3_4:+68225822 | Msa0516720:exon |
| TTTGGGACTAAATAGGTGTT+TGG | 0.379595 | 3_4:+68225829 | Msa0516720:exon |
| GTTTAAAATTACACATTTGT+TGG | 0.414296 | 3_4:+68225535 | Msa0516720:five_prime_UTR |
| TTTATGTCTAAGAAAGGTCT+AGG | 0.418606 | 3_4:-68225902 | None:intergenic |
| ACAGGGGTGAGACAAGAATA+AGG | 0.429745 | 3_4:+68225235 | None:intergenic |
| TTAAAATTTATGTCTAAGAA+AGG | 0.433401 | 3_4:-68225908 | None:intergenic |
| GCTTACAAGAGGGTCAATTT+GGG | 0.435870 | 3_4:+68225812 | Msa0516720:CDS |
| GTTGTAGTAAATTGAGGAGA+AGG | 0.436524 | 3_4:+68225510 | Msa0516720:intron |
| CTTATTCCAGGTTTCATCCA+TGG | 0.439084 | 3_4:+68225700 | Msa0516720:intron |
| TTGGAGACAAAATTATATAT+GGG | 0.456932 | 3_4:+68225264 | Msa0516720:five_prime_UTR |
| TTATTGCTCTTTACATGAAG+TGG | 0.459678 | 3_4:+68225734 | Msa0516720:CDS |
| TTGTAGTAAATTGAGGAGAA+GGG | 0.474580 | 3_4:+68225511 | Msa0516720:intron |
| TTATTCCAGGTTTCATCCAT+GGG | 0.496206 | 3_4:+68225701 | Msa0516720:intron |
| TAGTTTCATCTCCAGTGCAA+AGG | 0.555004 | 3_4:+68225568 | Msa0516720:CDS |
| TTCTTCAAGTCCCACTTGTG+AGG | 0.564284 | 3_4:-68225766 | None:intergenic |
| TTATGTCTAAGAAAGGTCTA+GGG | 0.603744 | 3_4:-68225901 | None:intergenic |
| CAATAACATCTATTCACCCA+TGG | 0.638860 | 3_4:-68225717 | None:intergenic |
| GGAGAACAAAGCCTCACAAG+TGG | 0.638950 | 3_4:+68225755 | Msa0516720:CDS |
| GAGAACAAAGCCTCACAAGT+GGG | 0.677160 | 3_4:+68225756 | Msa0516720:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTCAAGAATTGTTTTTAT+TGG | + | chr3_4:68225788-68225807 | Msa0516720:CDS | 15.0% |
| !! | TTAAAATTTATGTCTAAGAA+AGG | - | chr3_4:68225911-68225930 | None:intergenic | 15.0% |
| !!! | TTCTTAGACATAAATTTTAA+TGG | + | chr3_4:68225911-68225930 | Msa0516720:three_prime_UTR | 15.0% |
| !! | TTTGGAGACAAAATTATATA+TGG | + | chr3_4:68225263-68225282 | Msa0516720:five_prime_UTR | 20.0% |
| !! | TTGGAGACAAAATTATATAT+GGG | + | chr3_4:68225264-68225283 | Msa0516720:five_prime_UTR | 20.0% |
| !!! | TGAAAACGAAAAACAAATTT+TGG | - | chr3_4:68225431-68225450 | None:intergenic | 20.0% |
| !!! | TTTTTTGTTGTAGTAAATTG+AGG | + | chr3_4:68225504-68225523 | Msa0516720:intron | 20.0% |
| !!! | GTTTAAAATTACACATTTGT+TGG | + | chr3_4:68225535-68225554 | Msa0516720:five_prime_UTR | 20.0% |
| !! | CATAGCAATAAAAAATAACA+TGG | - | chr3_4:68225659-68225678 | None:intergenic | 20.0% |
| !!! | TAGTATTTTAAACTTATTCC+AGG | + | chr3_4:68225688-68225707 | Msa0516720:intron | 20.0% |
| ! | GTTAGAAATGAAATCAAATG+TGG | - | chr3_4:68225468-68225487 | None:intergenic | 25.0% |
| ! | AATAACATGGCATTATTTGA+AGG | - | chr3_4:68225646-68225665 | None:intergenic | 25.0% |
| GACAAGAATAAGGATGAATT+TGG | + | chr3_4:68225245-68225264 | Msa0516720:five_prime_UTR | 30.0% | |
| GAGACAAAATTATATATGGG+TGG | + | chr3_4:68225267-68225286 | Msa0516720:intron | 30.0% | |
| GAAATGAAATCAAATGTGGA+AGG | - | chr3_4:68225464-68225483 | None:intergenic | 30.0% | |
| TTGTAGTAAATTGAGGAGAA+GGG | + | chr3_4:68225511-68225530 | Msa0516720:intron | 30.0% | |
| !! | ATATTTTTCAACCTTTGCAC+TGG | - | chr3_4:68225582-68225601 | None:intergenic | 30.0% |
| TTATTGCTCTTTACATGAAG+TGG | + | chr3_4:68225734-68225753 | Msa0516720:CDS | 30.0% | |
| !! | TTTTTATTGGTGCTTACAAG+AGG | + | chr3_4:68225801-68225820 | Msa0516720:CDS | 30.0% |
| !! | TTTTATTGGTGCTTACAAGA+GGG | + | chr3_4:68225802-68225821 | Msa0516720:CDS | 30.0% |
| ! | TTTTATAGTGCGTAATCACA+AGG | + | chr3_4:68225868-68225887 | Msa0516720:three_prime_UTR | 30.0% |
| TTATGTCTAAGAAAGGTCTA+GGG | - | chr3_4:68225904-68225923 | None:intergenic | 30.0% | |
| TTTATGTCTAAGAAAGGTCT+AGG | - | chr3_4:68225905-68225924 | None:intergenic | 30.0% | |
| TGTTGAAGCAAAGAAATCAG+TGG | - | chr3_4:68225294-68225313 | None:intergenic | 35.0% | |
| GACTGATATCAACTGAGAAA+GGG | - | chr3_4:68225385-68225404 | None:intergenic | 35.0% | |
| AGACTGATATCAACTGAGAA+AGG | - | chr3_4:68225386-68225405 | None:intergenic | 35.0% | |
| GTTGTAGTAAATTGAGGAGA+AGG | + | chr3_4:68225510-68225529 | Msa0516720:intron | 35.0% | |
| GGCATTATTTGAAGGATTTG+AGG | - | chr3_4:68225638-68225657 | None:intergenic | 35.0% | |
| TTATTCCAGGTTTCATCCAT+GGG | + | chr3_4:68225701-68225720 | Msa0516720:intron | 35.0% | |
| CAATAACATCTATTCACCCA+TGG | - | chr3_4:68225720-68225739 | None:intergenic | 35.0% | |
| ! | TTTGGGACTAAATAGGTGTT+TGG | + | chr3_4:68225829-68225848 | Msa0516720:exon | 35.0% |
| GGGAAAAAACACAGTGCAAA+AGG | - | chr3_4:68225365-68225384 | None:intergenic | 40.0% | |
| TAGTTTCATCTCCAGTGCAA+AGG | + | chr3_4:68225568-68225587 | Msa0516720:CDS | 40.0% | |
| CTTATTCCAGGTTTCATCCA+TGG | + | chr3_4:68225700-68225719 | Msa0516720:intron | 40.0% | |
| TGCTTACAAGAGGGTCAATT+TGG | + | chr3_4:68225811-68225830 | Msa0516720:CDS | 40.0% | |
| GCTTACAAGAGGGTCAATTT+GGG | + | chr3_4:68225812-68225831 | Msa0516720:CDS | 40.0% | |
| ! | GGGTCAATTTGGGACTAAAT+AGG | + | chr3_4:68225822-68225841 | Msa0516720:exon | 40.0% |
| ATTCACCCATGGATGAAACC+TGG | - | chr3_4:68225709-68225728 | None:intergenic | 45.0% | |
| GAGAACAAAGCCTCACAAGT+GGG | + | chr3_4:68225756-68225775 | Msa0516720:CDS | 45.0% | |
| TTCTTCAAGTCCCACTTGTG+AGG | - | chr3_4:68225769-68225788 | None:intergenic | 45.0% | |
| GGAGAACAAAGCCTCACAAG+TGG | + | chr3_4:68225755-68225774 | Msa0516720:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_4 | gene | 68225236 | 68225948 | 68225236 | ID=Msa0516720;Name=Msa0516720 |
| chr3_4 | mRNA | 68225236 | 68225948 | 68225236 | ID=Msa0516720-mRNA-1;Parent=Msa0516720;Name=Msa0516720-mRNA-1;_AED=0.19;_eAED=0.19;_QI=93|1|1|1|0|0|3|109|58 |
| chr3_4 | exon | 68225236 | 68225285 | 68225236 | ID=Msa0516720-mRNA-1:exon:17946;Parent=Msa0516720-mRNA-1 |
| chr3_4 | exon | 68225517 | 68225606 | 68225517 | ID=Msa0516720-mRNA-1:exon:17947;Parent=Msa0516720-mRNA-1 |
| chr3_4 | exon | 68225710 | 68225948 | 68225710 | ID=Msa0516720-mRNA-1:exon:17948;Parent=Msa0516720-mRNA-1 |
| chr3_4 | five_prime_UTR | 68225236 | 68225285 | 68225236 | ID=Msa0516720-mRNA-1:five_prime_utr;Parent=Msa0516720-mRNA-1 |
| chr3_4 | five_prime_UTR | 68225517 | 68225559 | 68225517 | ID=Msa0516720-mRNA-1:five_prime_utr;Parent=Msa0516720-mRNA-1 |
| chr3_4 | CDS | 68225560 | 68225606 | 68225560 | ID=Msa0516720-mRNA-1:cds;Parent=Msa0516720-mRNA-1 |
| chr3_4 | CDS | 68225710 | 68225839 | 68225710 | ID=Msa0516720-mRNA-1:cds;Parent=Msa0516720-mRNA-1 |
| chr3_4 | three_prime_UTR | 68225840 | 68225948 | 68225840 | ID=Msa0516720-mRNA-1:three_prime_utr;Parent=Msa0516720-mRNA-1 |
| Gene Sequence |
| Protein sequence |
