AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0618530

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0618530 MtrunA17_Chr8g0383471 92.683 82 6 0 1 82 1 82 3.68e-44 137
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 48 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAACATTGATTATGATTTA+TGG 0.145840 4_2:+69474535 None:intergenic
AACTATAGATATTTGATATA+TGG 0.209688 4_2:+69475467 None:intergenic
AAACTAGGAGACTTTAATTA+TGG 0.215662 4_2:+69474965 None:intergenic
CTTGTGTTAGTGTCCCTTCA+TGG 0.254810 4_2:+69475067 None:intergenic
TTCCCTCAATGTTACGTTGT+TGG 0.341086 4_2:+69475703 None:intergenic
TGGCAAAACACTCAATTATC+AGG 0.352740 4_2:-69477974 Msa0618530:intron
CACTTGTAAATGTATAGTTT+TGG 0.364191 4_2:-69474729 Msa0618530:three_prime_UTR
ACCCTTTGTGTTCTTTAACT+TGG 0.379775 4_2:-69475676 Msa0618530:five_prime_UTR
TACTAAAGATGGAGCTGAAA+AGG 0.386508 4_2:-69475115 Msa0618530:CDS
AGGGGAGCATATATAAATAT+AGG 0.412997 4_2:-69474690 Msa0618530:three_prime_UTR
TTGAGAGTAATATTAAGATA+TGG 0.450623 4_2:-69475446 Msa0618530:exon
TGCATAAGATATGGCCTAGT+TGG 0.452766 4_2:+69475499 None:intergenic
ATAACTCTCCTCCATGCATA+TGG 0.467549 4_2:+69478009 None:intergenic
AAGCTCATAGATAGAATTGC+AGG 0.467654 4_2:+69474868 None:intergenic
TTTGTGTCCCTCGAAATTGT+TGG 0.468411 4_2:+69475729 None:intergenic
AGGAGACTTTAATTATGGAT+TGG 0.479268 4_2:+69474970 None:intergenic
AGACTTTAATTATGGATTGG+TGG 0.484159 4_2:+69474973 None:intergenic
ATGGATTGGTGGATTCTTTG+AGG 0.484420 4_2:+69474984 None:intergenic
TTTGTGGAGGTGAAAGCACC+AGG 0.494541 4_2:-69474785 Msa0618530:three_prime_UTR
GCAACAAGAAGGCGTTTGCT+TGG 0.499691 4_2:+69475415 None:intergenic
ATTGGTGGATTCTTTGAGGC+AGG 0.510445 4_2:+69474988 None:intergenic
GGTAATTAAGCACTGTGGAT+TGG 0.514972 4_2:+69474890 None:intergenic
ACTAAAGATGGAGCTGAAAA+GGG 0.528078 4_2:-69475114 Msa0618530:CDS
AGCCAACAACGTAACATTGA+GGG 0.530525 4_2:-69475705 Msa0618530:five_prime_UTR
TGGAAGTTATGAGAGTGTGA+GGG 0.536134 4_2:-69474709 Msa0618530:three_prime_UTR
TTGGAAGTTATGAGAGTGTG+AGG 0.537291 4_2:-69474710 Msa0618530:three_prime_UTR
TCCAAGTTAAAGAACACAAA+GGG 0.541459 4_2:+69475675 None:intergenic
GGAAGTTATGAGAGTGTGAG+GGG 0.541779 4_2:-69474708 Msa0618530:three_prime_UTR
ACATATGTCTGCATAAGGTA+AGG 0.543962 4_2:+69474606 None:intergenic
TTGTTTAAGCTAAACCATGA+AGG 0.545024 4_2:-69475081 Msa0618530:CDS
GGAGTTAAGTAATTTGCAAG+AGG 0.553059 4_2:+69475539 None:intergenic
TGATGACTTGTTGGATACAA+TGG 0.554869 4_2:-69475019 Msa0618530:CDS
ACAACGTAACATTGAGGGAA+TGG 0.556781 4_2:-69475700 Msa0618530:five_prime_UTR
AACTTGAGAGATACTAAAGA+TGG 0.564007 4_2:-69475126 Msa0618530:CDS
GAGTTAAGTAATTTGCAAGA+GGG 0.566314 4_2:+69475540 None:intergenic
TTCATCTTAACTACAGCTCA+AGG 0.570896 4_2:-69475376 Msa0618530:intron
GCACAGTGCCAACAATTTCG+AGG 0.572541 4_2:-69475737 Msa0618530:five_prime_UTR
TGTTTAAGCTAAACCATGAA+GGG 0.579354 4_2:-69475080 Msa0618530:CDS
AGAGTTATTCTATCAAGTCA+TGG 0.596916 4_2:-69477994 Msa0618530:five_prime_UTR
CACAGTGCCAACAATTTCGA+GGG 0.621204 4_2:-69475736 Msa0618530:five_prime_UTR
AAGCCAACAACGTAACATTG+AGG 0.634993 4_2:-69475706 Msa0618530:five_prime_UTR
ATCCAAGTTAAAGAACACAA+AGG 0.639483 4_2:+69475674 None:intergenic
TTGGGGAAGAGCCATATGCA+TGG 0.644947 4_2:-69478020 None:intergenic
TGATTAAGCATCAACAACCC+TGG 0.657692 4_2:+69474767 None:intergenic
AGCTCATAGATAGAATTGCA+GGG 0.664206 4_2:+69474869 None:intergenic
TTGTGGAGGTGAAAGCACCA+GGG 0.685604 4_2:-69474784 Msa0618530:three_prime_UTR
TGCAGGGTAATTAAGCACTG+TGG 0.694712 4_2:+69474885 None:intergenic
GGGAAGAGCCATATGCATGG+AGG 0.733072 4_2:-69478017 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGCTATAATAAAAAATTAT+AGG + chr4_2:69474782-69474801 None:intergenic 10.0%
!!! TTTTTATAGATTTTTTTTTG+AGG - chr4_2:69474901-69474920 Msa0618530:three_prime_UTR 10.0%
!!! AATATTTTTTTTGAGAAAAA+GGG - chr4_2:69475563-69475582 Msa0618530:five_prime_UTR 10.0%
!!! AATTTTTTATAATCTATAAG+AGG + chr4_2:69476068-69476087 None:intergenic 10.0%
!!! TACAATTTTAAAAATATTGA+GGG + chr4_2:69476262-69476281 None:intergenic 10.0%
!!! TTACAATTTTAAAAATATTG+AGG + chr4_2:69476263-69476282 None:intergenic 10.0%
!!! AAACATTATCATTAATTTTT+CGG + chr4_2:69476565-69476584 None:intergenic 10.0%
!! AATTAATATTTGTATATTTG+AGG - chr4_2:69477344-69477363 Msa0618530:intron 10.0%
!! TAAAAAAATATGTATTGTGA+TGG - chr4_2:69474656-69474675 Msa0618530:three_prime_UTR 15.0%
!!! TTTTCTTATATCAGATATAT+AGG - chr4_2:69474732-69474751 Msa0618530:three_prime_UTR 15.0%
!!! ATTAGAATTTTGATTCATTT+CGG - chr4_2:69475126-69475145 Msa0618530:CDS 15.0%
!! GATGTTATATATAGATAATT+GGG - chr4_2:69475176-69475195 Msa0618530:intron 15.0%
!!! CAATATTTTTTTTGAGAAAA+AGG - chr4_2:69475562-69475581 Msa0618530:five_prime_UTR 15.0%
!!! ATTTTATTAATGCAGATATT+TGG - chr4_2:69476772-69476791 Msa0618530:intron 15.0%
!! AACTATAGATATTTGATATA+TGG + chr4_2:69477073-69477092 None:intergenic 15.0%
!! AAAGAGAAAAAAATAAAACT+AGG + chr4_2:69477590-69477609 None:intergenic 15.0%
!! GCAAAATAGTTTAGTAATAT+TGG - chr4_2:69474798-69474817 Msa0618530:three_prime_UTR 20.0%
!!! AGTTTTAAAAATCTCTTGTT+CGG + chr4_2:69475081-69475100 None:intergenic 20.0%
!! GGATGTTATATATAGATAAT+TGG - chr4_2:69475175-69475194 Msa0618530:intron 20.0%
!! TTGTATGAAAGTTATTATCA+TGG + chr4_2:69475661-69475680 None:intergenic 20.0%
!! AATCCAGATTAAAAGAAAAA+CGG + chr4_2:69475819-69475838 None:intergenic 20.0%
!! TTAATCTGGATTTCTTAAAT+AGG - chr4_2:69475827-69475846 Msa0618530:intron 20.0%
!!! TTTTAACCTTTCAATTTTTG+AGG - chr4_2:69476743-69476762 Msa0618530:intron 20.0%
!!! TTTAACCTTTCAATTTTTGA+GGG - chr4_2:69476744-69476763 Msa0618530:intron 20.0%
!!! AAAACAATTGTTGTGATTTT+AGG + chr4_2:69476954-69476973 None:intergenic 20.0%
!! TTGAGAGTAATATTAAGATA+TGG - chr4_2:69477091-69477110 Msa0618530:intron 20.0%
!! GAAAACATCACATAAATTAT+AGG + chr4_2:69477370-69477389 None:intergenic 20.0%
!!! ATTTATGTGATGTTTTCTTT+TGG - chr4_2:69477373-69477392 Msa0618530:intron 20.0%
!!! ATTTTTGGTAGGAAAAAAAA+AGG - chr4_2:69477622-69477641 Msa0618530:intron 20.0%
!!! ATATTTTGAACTTTGAAGAT+AGG - chr4_2:69477704-69477723 Msa0618530:intron 20.0%
!!! TATTTTGAACTTTGAAGATA+GGG - chr4_2:69477705-69477724 Msa0618530:intron 20.0%
! GTATATCTCTTCTTTATTCA+TGG + chr4_2:69474709-69474728 None:intergenic 25.0%
! TATAGGAAACTTTCAAAACT+TGG + chr4_2:69474765-69474784 None:intergenic 25.0%
! TGAAAATCCTAGTTTAAACT+CGG + chr4_2:69475022-69475041 None:intergenic 25.0%
!!! GTTTTAAAAATCTCTTGTTC+GGG + chr4_2:69475080-69475099 None:intergenic 25.0%
! AAAAAAAGAGGTCCATATTA+GGG + chr4_2:69475205-69475224 None:intergenic 25.0%
! TAAAAAAAGAGGTCCATATT+AGG + chr4_2:69475206-69475225 None:intergenic 25.0%
!!! TAATATGGACCTCTTTTTTT+AGG - chr4_2:69475205-69475224 Msa0618530:intron 25.0%
!!! AATATGGACCTCTTTTTTTA+GGG - chr4_2:69475206-69475225 Msa0618530:intron 25.0%
! GAATGATGAAATTAAAGTTG+CGG + chr4_2:69475396-69475415 None:intergenic 25.0%
! GTTGATTATAGTTGTTTATG+TGG + chr4_2:69475634-69475653 None:intergenic 25.0%
!!! AAACCGTTTTTCTTTTAATC+TGG - chr4_2:69475813-69475832 Msa0618530:intron 25.0%
! TCAATATCTTCGTTAAAAAC+AGG + chr4_2:69475855-69475874 None:intergenic 25.0%
!! TTATGTGGTTTTTAACAGAA+AGG - chr4_2:69475879-69475898 Msa0618530:intron 25.0%
! CAATCAATAACCAATTATCT+AGG + chr4_2:69476014-69476033 None:intergenic 25.0%
!!! GAAATTTAGATTTTGACACA+AGG - chr4_2:69476373-69476392 Msa0618530:intron 25.0%
! TATCATAGTATATGTATCGA+TGG + chr4_2:69476531-69476550 None:intergenic 25.0%
!! AAACAAATAAACACGTTTTG+AGG + chr4_2:69476708-69476727 None:intergenic 25.0%
!!! TTAACCTTTCAATTTTTGAG+GGG - chr4_2:69476745-69476764 Msa0618530:intron 25.0%
!!! TTTTGAAGTTGCATAAGATA+TGG + chr4_2:69477050-69477069 None:intergenic 25.0%
!! TGCATCGTTCATTAATTTTA+AGG - chr4_2:69477224-69477243 Msa0618530:intron 25.0%
!! AGAAAAAAATGATGACTTGT+TGG - chr4_2:69477509-69477528 Msa0618530:intron 25.0%
! AAACTAGGAGACTTTAATTA+TGG + chr4_2:69477575-69477594 None:intergenic 25.0%
!! TTTCCTTAGAGAACAATTTT+TGG - chr4_2:69477607-69477626 Msa0618530:intron 25.0%
! AAAAATCTGTGATTTAAGTG+TGG + chr4_2:69477735-69477754 None:intergenic 25.0%
!! ACTTAAATCACAGATTTTTG+TGG - chr4_2:69477736-69477755 Msa0618530:intron 25.0%
!! CACTTGTAAATGTATAGTTT+TGG - chr4_2:69477808-69477827 Msa0618530:intron 25.0%
!! ATATACTAAGCATCTGTTTT+TGG + chr4_2:69477908-69477927 None:intergenic 25.0%
!! GGAACATTGATTATGATTTA+TGG + chr4_2:69478005-69478024 None:intergenic 25.0%
AGAGTTATTCTATCAAGTCA+TGG - chr4_2:69474543-69474562 Msa0618530:three_prime_UTR 30.0%
AGTAGATATTTGTACTGTGA+TGG - chr4_2:69474623-69474642 Msa0618530:three_prime_UTR 30.0%
TGATATACGTAGCATATGAT+AGG - chr4_2:69474830-69474849 Msa0618530:three_prime_UTR 30.0%
!! TTCGATTAGTTTCAGATAAG+GGG + chr4_2:69475055-69475074 None:intergenic 30.0%
!! CTTCGATTAGTTTCAGATAA+GGG + chr4_2:69475056-69475075 None:intergenic 30.0%
!! ACTTCGATTAGTTTCAGATA+AGG + chr4_2:69475057-69475076 None:intergenic 30.0%
AAAAAAGAGGTCCATATTAG+GGG + chr4_2:69475204-69475223 None:intergenic 30.0%
AGTTATATAGCAAGAACCAT+GGG + chr4_2:69475236-69475255 None:intergenic 30.0%
AAGTTATATAGCAAGAACCA+TGG + chr4_2:69475237-69475256 None:intergenic 30.0%
! TGCTATATAACTTGCAATAG+TGG - chr4_2:69475244-69475263 Msa0618530:intron 30.0%
! GCTATATAACTTGCAATAGT+GGG - chr4_2:69475245-69475264 Msa0618530:intron 30.0%
! TTGTGAATTAGATGATTTGC+TGG + chr4_2:69475348-69475367 None:intergenic 30.0%
AAATTAAAGTTGCGGAATCA+AGG + chr4_2:69475388-69475407 None:intergenic 30.0%
TTATGAACCAGAAACCATTT+GGG + chr4_2:69475461-69475480 None:intergenic 30.0%
TTTATGAACCAGAAACCATT+TGG + chr4_2:69475462-69475481 None:intergenic 30.0%
TTCAAATTCTACAGTTGCAA+TGG + chr4_2:69475514-69475533 None:intergenic 30.0%
GCAACTGTAGAATTTGAATA+AGG - chr4_2:69475516-69475535 Msa0618530:five_prime_UTR 30.0%
! AAGCTTGTCTTTTGTGTTTA+AGG + chr4_2:69475542-69475561 None:intergenic 30.0%
AACAGTTGGAATAACAATAG+TGG + chr4_2:69475595-69475614 None:intergenic 30.0%
GTTAACCGGAACAAAATTAA+TGG - chr4_2:69475698-69475717 Msa0618530:five_prime_UTR 30.0%
! GAAGATATTGAAGCATTATG+TGG - chr4_2:69475864-69475883 Msa0618530:intron 30.0%
!!! ATTATGGACCCCTTTTTTAA+AGG + chr4_2:69475983-69476002 None:intergenic 30.0%
TTAAAAAAGGGGTCCATAAT+AGG - chr4_2:69475983-69476002 Msa0618530:intron 30.0%
TAAAAAAGGGGTCCATAATA+GGG - chr4_2:69475984-69476003 Msa0618530:intron 30.0%
CCTTATAATGTGAGGTTATT+TGG + chr4_2:69476216-69476235 None:intergenic 30.0%
CCAAATAACCTCACATTATA+AGG - chr4_2:69476213-69476232 Msa0618530:intron 30.0%
CATTTAACTAGCACAAGATT+TGG - chr4_2:69476452-69476471 Msa0618530:intron 30.0%
TTTGTCAGAATATATGTGCA+AGG - chr4_2:69476582-69476601 Msa0618530:intron 30.0%
!!! AAATAAACACGTTTTGAGGA+GGG + chr4_2:69476704-69476723 None:intergenic 30.0%
!! TAACCTTTCAATTTTTGAGG+GGG - chr4_2:69476746-69476765 Msa0618530:intron 30.0%
TCCAAGTTAAAGAACACAAA+GGG + chr4_2:69476865-69476884 None:intergenic 30.0%
ATCCAAGTTAAAGAACACAA+AGG + chr4_2:69476866-69476885 None:intergenic 30.0%
! GAGTTAAGTAATTTGCAAGA+GGG + chr4_2:69477000-69477019 None:intergenic 30.0%
!! CTAGTTGGTGTCTTTTTATA+GGG + chr4_2:69477026-69477045 None:intergenic 30.0%
TCAACAAAAATGCAACAAGA+AGG + chr4_2:69477136-69477155 None:intergenic 30.0%
AACTTGAGAGATACTAAAGA+TGG - chr4_2:69477411-69477430 Msa0618530:intron 30.0%
TTGTTTAAGCTAAACCATGA+AGG - chr4_2:69477456-69477475 Msa0618530:intron 30.0%
TGTTTAAGCTAAACCATGAA+GGG - chr4_2:69477457-69477476 Msa0618530:intron 30.0%
!! AGACTTTAATTATGGATTGG+TGG + chr4_2:69477567-69477586 None:intergenic 30.0%
AGGAGACTTTAATTATGGAT+TGG + chr4_2:69477570-69477589 None:intergenic 30.0%
CTACCAAAAATTGTTCTCTA+AGG + chr4_2:69477613-69477632 None:intergenic 30.0%
!!! CTTAGAGAACAATTTTTGGT+AGG - chr4_2:69477611-69477630 Msa0618530:intron 30.0%
! TAAATCACAGATTTTTGTGG+AGG - chr4_2:69477739-69477758 Msa0618530:intron 30.0%
! AGGGGAGCATATATAAATAT+AGG - chr4_2:69477847-69477866 Msa0618530:intron 30.0%
CAAAAACATATGTCTGCATA+AGG + chr4_2:69477939-69477958 None:intergenic 30.0%
TGGCAAAACACTCAATTATC+AGG - chr4_2:69474563-69474582 Msa0618530:three_prime_UTR 35.0%
CATTGATCCGAGTTTAAACT+AGG - chr4_2:69475012-69475031 Msa0618530:CDS 35.0%
CACTTCCTTCTCAATTTACA+TGG - chr4_2:69475274-69475293 Msa0618530:intron 35.0%
TCCTTCTCAATTTACATGGT+TGG - chr4_2:69475278-69475297 Msa0618530:intron 35.0%
AGTCACAGTAAAGATTCATG+TGG + chr4_2:69475307-69475326 None:intergenic 35.0%
TTAAACATAGCCATCCCAAA+TGG - chr4_2:69475444-69475463 Msa0618530:exon 35.0%
AGCGTGAAAACATTAACAGT+TGG + chr4_2:69475609-69475628 None:intergenic 35.0%
TTTGTTCCGGTTAACAAACT+TGG + chr4_2:69475693-69475712 None:intergenic 35.0%
! TGGAGCCATTAATTTTGTTC+CGG + chr4_2:69475706-69475725 None:intergenic 35.0%
GAACAAAATTAATGGCTCCA+TGG - chr4_2:69475706-69475725 Msa0618530:five_prime_UTR 35.0%
TCTGCACTCTGATATACATT+TGG + chr4_2:69475914-69475933 None:intergenic 35.0%
! ATGGGTGGACCTTTAAAAAA+GGG - chr4_2:69475971-69475990 Msa0618530:intron 35.0%
AAAAAAGGGGTCCATAATAG+GGG - chr4_2:69475985-69476004 Msa0618530:intron 35.0%
CAAAAGCTCCTTATAATGTG+AGG + chr4_2:69476224-69476243 None:intergenic 35.0%
ACAAGATTTGGATTTGTGCA+TGG - chr4_2:69476464-69476483 Msa0618530:intron 35.0%
! TGACTTGTGAGATTGGAAAA+GGG - chr4_2:69476629-69476648 Msa0618530:intron 35.0%
GAATCACATATCACCCTTAT+AGG - chr4_2:69476651-69476670 Msa0618530:intron 35.0%
!!! AATAAACACGTTTTGAGGAG+GGG + chr4_2:69476703-69476722 None:intergenic 35.0%
!!! CAAATAAACACGTTTTGAGG+AGG + chr4_2:69476705-69476724 None:intergenic 35.0%
ACCCTTTGTGTTCTTTAACT+TGG - chr4_2:69476861-69476880 Msa0618530:intron 35.0%
!! ACACTTTTTGCTTCGATTAC+AGG + chr4_2:69476889-69476908 None:intergenic 35.0%
! GGAGTTAAGTAATTTGCAAG+AGG + chr4_2:69477001-69477020 None:intergenic 35.0%
!! TTGGTGTCTTTTTATAGGGA+GGG + chr4_2:69477022-69477041 None:intergenic 35.0%
!! CCTAGTTGGTGTCTTTTTAT+AGG + chr4_2:69477027-69477046 None:intergenic 35.0%
CCTATAAAAAGACACCAACT+AGG - chr4_2:69477024-69477043 Msa0618530:intron 35.0%
TTCATCTTAACTACAGCTCA+AGG - chr4_2:69477161-69477180 Msa0618530:intron 35.0%
TACTAAAGATGGAGCTGAAA+AGG - chr4_2:69477422-69477441 Msa0618530:intron 35.0%
ACTAAAGATGGAGCTGAAAA+GGG - chr4_2:69477423-69477442 Msa0618530:intron 35.0%
!! TGATGACTTGTTGGATACAA+TGG - chr4_2:69477518-69477537 Msa0618530:intron 35.0%
AGCTCATAGATAGAATTGCA+GGG + chr4_2:69477671-69477690 None:intergenic 35.0%
AAGCTCATAGATAGAATTGC+AGG + chr4_2:69477672-69477691 None:intergenic 35.0%
ACATATGTCTGCATAAGGTA+AGG + chr4_2:69477934-69477953 None:intergenic 35.0%
ATAACTCTCCTCCATGCATA+TGG + chr4_2:69474531-69474550 None:intergenic 40.0%
ATAATTGGGAGCCCCTAATA+TGG - chr4_2:69475190-69475209 Msa0618530:intron 40.0%
TATATAGCAAGAACCATGGG+TGG + chr4_2:69475233-69475252 None:intergenic 40.0%
GCCAACCATGTAAATTGAGA+AGG + chr4_2:69475282-69475301 None:intergenic 40.0%
CACAGTAAAGATTCATGTGG+AGG + chr4_2:69475304-69475323 None:intergenic 40.0%
GAATCTCAGTTCGGCTTAAA+GGG + chr4_2:69475492-69475511 None:intergenic 40.0%
GTTGCAATGGAATCTCAGTT+CGG + chr4_2:69475501-69475520 None:intergenic 40.0%
!! CTGAAGCCAAGTTTGTTAAC+CGG - chr4_2:69475684-69475703 Msa0618530:five_prime_UTR 40.0%
AATTAATGGCTCCATGGATC+TGG - chr4_2:69475712-69475731 Msa0618530:five_prime_UTR 40.0%
ATTAATGGCTCCATGGATCT+GGG - chr4_2:69475713-69475732 Msa0618530:five_prime_UTR 40.0%
TGCAGAGAAACGTCATGAAT+CGG - chr4_2:69475747-69475766 Msa0618530:five_prime_UTR 40.0%
GAGTAATTACCTGCTATTGC+AGG - chr4_2:69475932-69475951 Msa0618530:intron 40.0%
! CTATTGCAGGTCATTTGCTA+AGG - chr4_2:69475945-69475964 Msa0618530:intron 40.0%
!!! CTTTTTTAAAGGTCCACCCA+TGG + chr4_2:69475972-69475991 None:intergenic 40.0%
! CATGGGTGGACCTTTAAAAA+AGG - chr4_2:69475970-69475989 Msa0618530:intron 40.0%
! TGGGTGGACCTTTAAAAAAG+GGG - chr4_2:69475972-69475991 Msa0618530:intron 40.0%
TATCTAGGAAGCCCCTATTA+TGG + chr4_2:69475999-69476018 None:intergenic 40.0%
ATAGGGGCTTCCTAGATAAT+TGG - chr4_2:69476001-69476020 Msa0618530:intron 40.0%
GTCTATGTGCACGATTATCA+CGG - chr4_2:69476498-69476517 Msa0618530:intron 40.0%
ATCTCACAAGTCACAACACA+TGG + chr4_2:69476622-69476641 None:intergenic 40.0%
! TGTGTTGTGACTTGTGAGAT+TGG - chr4_2:69476622-69476641 Msa0618530:intron 40.0%
! GTGACTTGTGAGATTGGAAA+AGG - chr4_2:69476628-69476647 Msa0618530:intron 40.0%
TGCATACAGGAGACCTATAA+GGG + chr4_2:69476667-69476686 None:intergenic 40.0%
TTGCATACAGGAGACCTATA+AGG + chr4_2:69476668-69476687 None:intergenic 40.0%
TTATAGGTCTCCTGTATGCA+AGG - chr4_2:69476667-69476686 Msa0618530:intron 40.0%
!!! ATAAACACGTTTTGAGGAGG+GGG + chr4_2:69476702-69476721 None:intergenic 40.0%
ACACCCCCTCAAAAATTGAA+AGG + chr4_2:69476752-69476771 None:intergenic 40.0%
TTTGTGTCCCTCGAAATTGT+TGG + chr4_2:69476811-69476830 None:intergenic 40.0%
AAGCCAACAACGTAACATTG+AGG - chr4_2:69476831-69476850 Msa0618530:intron 40.0%
AGCCAACAACGTAACATTGA+GGG - chr4_2:69476832-69476851 Msa0618530:intron 40.0%
TTCCCTCAATGTTACGTTGT+TGG + chr4_2:69476837-69476856 None:intergenic 40.0%
ACAACGTAACATTGAGGGAA+TGG - chr4_2:69476837-69476856 Msa0618530:intron 40.0%
!!! AAGTGTTGTCATTTTCAGCG+AGG - chr4_2:69476903-69476922 Msa0618530:intron 40.0%
!! GTTGGTGTCTTTTTATAGGG+AGG + chr4_2:69477023-69477042 None:intergenic 40.0%
TGCATAAGATATGGCCTAGT+TGG + chr4_2:69477041-69477060 None:intergenic 40.0%
ATTAATGAACGATGCAGCAG+TGG + chr4_2:69477220-69477239 None:intergenic 40.0%
!! ATGGATTGGTGGATTCTTTG+AGG + chr4_2:69477556-69477575 None:intergenic 40.0%
! GGTAATTAAGCACTGTGGAT+TGG + chr4_2:69477650-69477669 None:intergenic 40.0%
TGATTAAGCATCAACAACCC+TGG + chr4_2:69477773-69477792 None:intergenic 40.0%
! TTGGAAGTTATGAGAGTGTG+AGG - chr4_2:69477827-69477846 Msa0618530:intron 40.0%
! TGGAAGTTATGAGAGTGTGA+GGG - chr4_2:69477828-69477847 Msa0618530:intron 40.0%
TGGGTGGACCCTAAAAAAAG+AGG + chr4_2:69475217-69475236 None:intergenic 45.0%
!!! CTTTTTTTAGGGTCCACCCA+TGG - chr4_2:69475217-69475236 Msa0618530:intron 45.0%
TAGCCATCCCAAATGGTTTC+TGG - chr4_2:69475451-69475470 Msa0618530:five_prime_UTR 45.0%
GAACCAGAAACCATTTGGGA+TGG + chr4_2:69475457-69475476 None:intergenic 45.0%
AGTTCGGCTTAAAGGGAATG+AGG + chr4_2:69475485-69475504 None:intergenic 45.0%
GGAATCTCAGTTCGGCTTAA+AGG + chr4_2:69475493-69475512 None:intergenic 45.0%
CGGTTAACAAACTTGGCTTC+AGG + chr4_2:69475686-69475705 None:intergenic 45.0%
AGAGAAACGTCATGAATCGG+TGG - chr4_2:69475750-69475769 Msa0618530:five_prime_UTR 45.0%
ATCGGTGGTTTCAGAATCAG+CGG - chr4_2:69475765-69475784 Msa0618530:intron 45.0%
AGCAAATGACCTGCAATAGC+AGG + chr4_2:69475944-69475963 None:intergenic 45.0%
! AGGTCATTTGCTAAGGACCA+TGG - chr4_2:69475952-69475971 Msa0618530:intron 45.0%
! GGTCATTTGCTAAGGACCAT+GGG - chr4_2:69475953-69475972 Msa0618530:intron 45.0%
TATGTGCACGATTATCACGG+TGG - chr4_2:69476501-69476520 Msa0618530:intron 45.0%
!!! TAAACACGTTTTGAGGAGGG+GGG + chr4_2:69476701-69476720 None:intergenic 45.0%
CACAGTGCCAACAATTTCGA+GGG - chr4_2:69476801-69476820 Msa0618530:intron 45.0%
CTTGTGTTAGTGTCCCTTCA+TGG + chr4_2:69477473-69477492 None:intergenic 45.0%
!! ATTGGTGGATTCTTTGAGGC+AGG + chr4_2:69477552-69477571 None:intergenic 45.0%
! TGCAGGGTAATTAAGCACTG+TGG + chr4_2:69477655-69477674 None:intergenic 45.0%
! GGAAGTTATGAGAGTGTGAG+GGG - chr4_2:69477829-69477848 Msa0618530:intron 45.0%
!! CTATTATTTAAAAAAATATA+TGG + chr4_2:69474961-69474980 None:intergenic 5.0%
!!! AAAAATTAAGATTTTTATTT+TGG - chr4_2:69475154-69475173 Msa0618530:intron 5.0%
!!! AAAAAATATCTAAATTTTTA+AGG + chr4_2:69476100-69476119 None:intergenic 5.0%
AACATCCCATCCCAGATCCA+TGG + chr4_2:69475726-69475745 None:intergenic 50.0%
! CATTTGCTAAGGACCATGGG+TGG - chr4_2:69475956-69475975 Msa0618530:intron 50.0%
GGTCTCCTGTATGCAAGGAA+TGG - chr4_2:69476672-69476691 Msa0618530:intron 50.0%
!!! AAACACGTTTTGAGGAGGGG+GGG + chr4_2:69476700-69476719 None:intergenic 50.0%
GCACAGTGCCAACAATTTCG+AGG - chr4_2:69476800-69476819 Msa0618530:intron 50.0%
! ATTTTCAGCGAGGACAGAGC+AGG - chr4_2:69476913-69476932 Msa0618530:intron 50.0%
!! GCAACAAGAAGGCGTTTGCT+TGG + chr4_2:69477125-69477144 None:intergenic 50.0%
ATGAACGATGCAGCAGTGGT+AGG + chr4_2:69477216-69477235 None:intergenic 50.0%
!! TTTGTGGAGGTGAAAGCACC+AGG - chr4_2:69477752-69477771 Msa0618530:intron 50.0%
!! TTGTGGAGGTGAAAGCACCA+GGG - chr4_2:69477753-69477772 Msa0618530:intron 50.0%
ATGGCTCCATGGATCTGGGA+TGG - chr4_2:69475717-69475736 Msa0618530:five_prime_UTR 55.0%
! TGGCTCCATGGATCTGGGAT+GGG - chr4_2:69475718-69475737 Msa0618530:five_prime_UTR 55.0%
GGGCTCCATTCCTTGCATAC+AGG + chr4_2:69476680-69476699 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 69474527 69478032 69474527 ID=Msa0618530;Name=Msa0618530
chr4_2 mRNA 69474527 69478032 69474527 ID=Msa0618530-mRNA-1;Parent=Msa0618530;Name=Msa0618530-mRNA-1;_AED=0.02;_eAED=0.02;_QI=381|1|1|1|0.5|0.33|3|451|82
chr4_2 exon 69474527 69475153 69474527 ID=Msa0618530-mRNA-1:exon:20073;Parent=Msa0618530-mRNA-1
chr4_2 exon 69475377 69475772 69475377 ID=Msa0618530-mRNA-1:exon:20072;Parent=Msa0618530-mRNA-1
chr4_2 exon 69477975 69478032 69477975 ID=Msa0618530-mRNA-1:exon:20071;Parent=Msa0618530-mRNA-1
chr4_2 five_prime_UTR 69477975 69478032 69477975 ID=Msa0618530-mRNA-1:five_prime_utr;Parent=Msa0618530-mRNA-1
chr4_2 five_prime_UTR 69475450 69475772 69475450 ID=Msa0618530-mRNA-1:five_prime_utr;Parent=Msa0618530-mRNA-1
chr4_2 CDS 69475377 69475449 69475377 ID=Msa0618530-mRNA-1:cds;Parent=Msa0618530-mRNA-1
chr4_2 CDS 69474978 69475153 69474978 ID=Msa0618530-mRNA-1:cds;Parent=Msa0618530-mRNA-1
chr4_2 three_prime_UTR 69474527 69474977 69474527 ID=Msa0618530-mRNA-1:three_prime_utr;Parent=Msa0618530-mRNA-1
Gene Sequence

>Msa0618530

ATGGCTATTGTACCAAGCAAACGCCTTCTTGTTGCATTTTTGTTGATTTGCTTCATCTTAACTACAGCTCAAGCTAGAAACTTGAGAGATACTAAAGATGGAGCTGAAAAGGGTCAAAGTAGCTTGTTTAAGCTAAACCATGAAGGGACACTAACACAAGAAAAAACACAAACACAAGAAAAAAATGATGACTTGTTGGATACAATGGACTACACACCTGCCTCAAAGAATCCACCAATCCATAATTAA
Protein sequence

>Msa0618530

MAIVPSKRLLVAFLLICFILTTAQARNLRDTKDGAEKGQSSLFKLNHEGTLTQEKTQTQEKNDDLLDTMDYTPASKNPPIHN*