AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0568600

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0568600 MtrunA17_Chr8g0383471 93.902 82 5 0 1 82 1 82 2.01e-44 137
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 46 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAACATTGATTATGATTTA+TGG 0.145840 4_1:-70231979 None:intergenic
AACTATAGATATTTGATATA+TGG 0.209688 4_1:-70231049 None:intergenic
AAACTAGGAGACTTTAATTA+TGG 0.215662 4_1:-70231551 None:intergenic
CTTGTGTTTGTGTCCCTTCA+TGG 0.225941 4_1:-70231449 None:intergenic
TTCCCTCAATGTTACGTTGT+TGG 0.341086 4_1:-70230813 None:intergenic
TGGCAAAACACTCAATTATC+AGG 0.352740 4_1:+70228535 Msa0568600:five_prime_UTR
CACTTGTAAATGTATAGTTT+TGG 0.364191 4_1:+70231785 Msa0568600:three_prime_UTR
ACCCTTTGTGTTCTTTAACT+TGG 0.379775 4_1:+70230840 Msa0568600:five_prime_UTR
TACTAAAGATGGAGCTGAAA+AGG 0.386508 4_1:+70231401 Msa0568600:CDS
AGGGGAGCATATATAAATAT+AGG 0.412997 4_1:+70231824 Msa0568600:three_prime_UTR
TTGAGAGTAATATTAAGATA+TGG 0.450623 4_1:+70231070 Msa0568600:exon
TGCATAAGATATGGCCTAGT+TGG 0.452766 4_1:-70231017 None:intergenic
ATAACTCTCCTCCATGCATA+TGG 0.467549 4_1:-70228500 None:intergenic
AAGCTCATAGATAGAATTGC+AGG 0.467654 4_1:-70231646 None:intergenic
TTTGTGTCCCTCGAAATTGT+TGG 0.468411 4_1:-70230787 None:intergenic
AGGAGACTTTAATTATGGAT+TGG 0.479268 4_1:-70231546 None:intergenic
AGACTTTAATTATGGATTGG+TGG 0.484159 4_1:-70231543 None:intergenic
ATGGATTGGTGGATTCTTTG+AGG 0.484420 4_1:-70231532 None:intergenic
GCAACAAGAAGGCGTTTGCT+TGG 0.499691 4_1:-70231101 None:intergenic
ATTGGTGGATTCTTTGAGGC+AGG 0.510445 4_1:-70231528 None:intergenic
GGTAATTAAGCACTGTGGAT+TGG 0.514972 4_1:-70231624 None:intergenic
ACTAAAGATGGAGCTGAAAA+GGG 0.528078 4_1:+70231402 Msa0568600:CDS
AGCCAACAACGTAACATTGA+GGG 0.530525 4_1:+70230811 Msa0568600:five_prime_UTR
TGGAAGTTATGAGAGTGTGA+GGG 0.536134 4_1:+70231805 Msa0568600:three_prime_UTR
TTGGAAGTTATGAGAGTGTG+AGG 0.537291 4_1:+70231804 Msa0568600:three_prime_UTR
TCCAAGTTAAAGAACACAAA+GGG 0.541459 4_1:-70230841 None:intergenic
GGAAGTTATGAGAGTGTGAG+GGG 0.541779 4_1:+70231806 Msa0568600:three_prime_UTR
ACATATGTCTGCATAAGGTA+AGG 0.543962 4_1:-70231908 None:intergenic
TTGTTTAAGCTAAACCATGA+AGG 0.545024 4_1:+70231435 Msa0568600:CDS
GGAGTTAAGTAATTTGCAAG+AGG 0.553059 4_1:-70230977 None:intergenic
TGATGACTTGTTGGATACAA+TGG 0.554869 4_1:+70231497 Msa0568600:CDS
ACAACGTAACATTGAGGGAA+TGG 0.556781 4_1:+70230816 Msa0568600:five_prime_UTR
AACTTGAGAGATACTAAAGA+TGG 0.564007 4_1:+70231390 Msa0568600:CDS
GAGTTAAGTAATTTGCAAGA+GGG 0.566314 4_1:-70230976 None:intergenic
TTCATCTTAACTACAGCTCA+AGG 0.570896 4_1:+70231140 Msa0568600:CDS
GCACAGTGCCAACAATTTCG+AGG 0.572541 4_1:+70230779 Msa0568600:five_prime_UTR
TGTTTAAGCTAAACCATGAA+GGG 0.579354 4_1:+70231436 Msa0568600:CDS
AGAGTTATTCTATCAAGTCA+TGG 0.596916 4_1:+70228515 Msa0568600:five_prime_UTR
CACAGTGCCAACAATTTCGA+GGG 0.621204 4_1:+70230780 Msa0568600:five_prime_UTR
AAGCCAACAACGTAACATTG+AGG 0.634993 4_1:+70230810 Msa0568600:five_prime_UTR
ATCCAAGTTAAAGAACACAA+AGG 0.639483 4_1:-70230842 None:intergenic
TTGGGGAAGAGCCATATGCA+TGG 0.644947 4_1:+70228489 None:intergenic
TGATTAAGCATCAACAACAC+TGG 0.649291 4_1:-70231747 None:intergenic
AGCTCATAGATAGAATTGCA+GGG 0.664206 4_1:-70231645 None:intergenic
TGCAGGGTAATTAAGCACTG+TGG 0.694712 4_1:-70231629 None:intergenic
GGGAAGAGCCATATGCATGG+AGG 0.733072 4_1:+70228492 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGCTATAATAAAAAATTAT+AGG - chr4_1:70228754-70228773 None:intergenic 10.0%
!!! TTTTTATAGATTTTTTTTTG+AGG + chr4_1:70228873-70228892 Msa0568600:intron 10.0%
!!! AATATTTTTTTTGAGAAAAA+GGG + chr4_1:70229535-70229554 Msa0568600:intron 10.0%
!!! AATTTTTTATAATCTATAAG+AGG - chr4_1:70230045-70230064 None:intergenic 10.0%
!!! TACAATTTTAAAAATATTGA+GGG - chr4_1:70230240-70230259 None:intergenic 10.0%
!!! TTACAATTTTAAAAATATTG+AGG - chr4_1:70230241-70230260 None:intergenic 10.0%
!! AATTAATATTTGTATATTTG+AGG + chr4_1:70231323-70231342 Msa0568600:intron 10.0%
!! TAAAAAAATATGTATTGTGA+TGG + chr4_1:70228628-70228647 Msa0568600:intron 15.0%
!!! TTTTCTTATATCAGATATAT+AGG + chr4_1:70228704-70228723 Msa0568600:intron 15.0%
!!! ATTAGAATTTTGATTCATTT+CGG + chr4_1:70229097-70229116 Msa0568600:intron 15.0%
!! GATGTTATATATAGATAATT+GGG + chr4_1:70229147-70229166 Msa0568600:intron 15.0%
!!! CAATATTTTTTTTGAGAAAA+AGG + chr4_1:70229534-70229553 Msa0568600:intron 15.0%
!!! AAACATTATCATTGATTTTT+CGG - chr4_1:70230543-70230562 None:intergenic 15.0%
!!! ATTTTATTAATGCAGATATT+TGG + chr4_1:70230751-70230770 Msa0568600:intron 15.0%
!! AACTATAGATATTTGATATA+TGG - chr4_1:70231052-70231071 None:intergenic 15.0%
!!! AATTTTTGGTAGAAAAAAAA+AGG + chr4_1:70231599-70231618 Msa0568600:three_prime_UTR 15.0%
!! GCAAAATAGTTTAGTAATAT+TGG + chr4_1:70228770-70228789 Msa0568600:intron 20.0%
!!! AGTTTTAAAAATCTCTTGTT+CGG - chr4_1:70229052-70229071 None:intergenic 20.0%
!! GGATGTTATATATAGATAAT+TGG + chr4_1:70229146-70229165 Msa0568600:intron 20.0%
!! AAAAAAAGAGGTTCATATTA+GGG - chr4_1:70229176-70229195 None:intergenic 20.0%
!! TAAAAAAAGAGGTTCATATT+AGG - chr4_1:70229177-70229196 None:intergenic 20.0%
!!! TAATATGAACCTCTTTTTTT+AGG + chr4_1:70229176-70229195 Msa0568600:intron 20.0%
!!! AATATGAACCTCTTTTTTTA+GGG + chr4_1:70229177-70229196 Msa0568600:intron 20.0%
!! TTGTATGAAAGTTATTATCA+TGG - chr4_1:70229633-70229652 None:intergenic 20.0%
!! AATCCAGATTAAAAGAAAAA+CGG - chr4_1:70229792-70229811 None:intergenic 20.0%
!! TTAATCTGGATTTCTTAAAT+AGG + chr4_1:70229800-70229819 Msa0568600:intron 20.0%
!!! TTTTAACCTTTCAATTTTTG+AGG + chr4_1:70230722-70230741 Msa0568600:intron 20.0%
!!! TTTAACCTTTCAATTTTTGA+GGG + chr4_1:70230723-70230742 Msa0568600:intron 20.0%
!!! AAAACAATTGTTGTGATTTT+AGG - chr4_1:70230933-70230952 None:intergenic 20.0%
!! TTGAGAGTAATATTAAGATA+TGG + chr4_1:70231070-70231089 Msa0568600:exon 20.0%
!! GAAAACATCACATAAATTAT+AGG - chr4_1:70231349-70231368 None:intergenic 20.0%
!!! ATTTATGTGATGTTTTCTTT+TGG + chr4_1:70231352-70231371 Msa0568600:intron 20.0%
!! GAAAGAGAAAAAATAAAACT+AGG - chr4_1:70231569-70231588 None:intergenic 20.0%
!!! ATATTTTGAACTTTGAAGAT+AGG + chr4_1:70231681-70231700 Msa0568600:three_prime_UTR 20.0%
!!! TATTTTGAACTTTGAAGATA+GGG + chr4_1:70231682-70231701 Msa0568600:three_prime_UTR 20.0%
! GTATATCTCTTCTTTATTCA+TGG - chr4_1:70228681-70228700 None:intergenic 25.0%
! TATAGGAAACTTTCAAAACT+TGG - chr4_1:70228737-70228756 None:intergenic 25.0%
! TGAAAATCCTAGTTTAAACT+CGG - chr4_1:70228994-70229013 None:intergenic 25.0%
!!! GTTTTAAAAATCTCTTGTTC+GGG - chr4_1:70229051-70229070 None:intergenic 25.0%
! AAAAAAGAGGTTCATATTAG+GGG - chr4_1:70229175-70229194 None:intergenic 25.0%
! AAATTAAAGTTGCGAAATCA+AGG - chr4_1:70229360-70229379 None:intergenic 25.0%
! GTTGATTATAGTTGTTTATG+TGG - chr4_1:70229606-70229625 None:intergenic 25.0%
!!! AAACCGTTTTTCTTTTAATC+TGG + chr4_1:70229786-70229805 Msa0568600:intron 25.0%
! TCAATATCTTCGTTAAAAAC+AGG - chr4_1:70229828-70229847 None:intergenic 25.0%
!! TTATGTGGTTTTTAACAGAA+AGG + chr4_1:70229852-70229871 Msa0568600:intron 25.0%
! CAATCAATAACCAATTATCT+AGG - chr4_1:70229987-70230006 None:intergenic 25.0%
!!! GAAATTTAGATTTTGACACA+AGG + chr4_1:70230351-70230370 Msa0568600:intron 25.0%
! TATCATAGTATATGTATCGA+TGG - chr4_1:70230509-70230528 None:intergenic 25.0%
! TTTGTCAGAATATATATGCA+AGG + chr4_1:70230560-70230579 Msa0568600:intron 25.0%
!! AAACAAATAAACACGTTTTG+AGG - chr4_1:70230687-70230706 None:intergenic 25.0%
!!! TTAACCTTTCAATTTTTGAG+GGG + chr4_1:70230724-70230743 Msa0568600:intron 25.0%
!!! TTTTGAAGTTGCATAAGATA+TGG - chr4_1:70231029-70231048 None:intergenic 25.0%
!! TGCATCGTTCATTAATTTTA+AGG + chr4_1:70231203-70231222 Msa0568600:intron 25.0%
!! AGAAAAAAATGATGACTTGT+TGG + chr4_1:70231488-70231507 Msa0568600:CDS 25.0%
! AAACTAGGAGACTTTAATTA+TGG - chr4_1:70231554-70231573 None:intergenic 25.0%
!! TTTCCTTAGAGAACAATTTT+TGG + chr4_1:70231585-70231604 Msa0568600:three_prime_UTR 25.0%
! AAAAATCTGTGATTTAAGTG+TGG - chr4_1:70231712-70231731 None:intergenic 25.0%
!! ACTTAAATCACAGATTTTTG+TGG + chr4_1:70231713-70231732 Msa0568600:three_prime_UTR 25.0%
!! CACTTGTAAATGTATAGTTT+TGG + chr4_1:70231785-70231804 Msa0568600:three_prime_UTR 25.0%
!! ATATACTAAGCATCTGTTTT+TGG - chr4_1:70231885-70231904 None:intergenic 25.0%
!! GGAACATTGATTATGATTTA+TGG - chr4_1:70231982-70232001 None:intergenic 25.0%
AGAGTTATTCTATCAAGTCA+TGG + chr4_1:70228515-70228534 Msa0568600:five_prime_UTR 30.0%
AGTAGATATTTGTACTGTGA+TGG + chr4_1:70228595-70228614 Msa0568600:intron 30.0%
TGATATACGTAGCATATGAT+AGG + chr4_1:70228802-70228821 Msa0568600:intron 30.0%
!! TTCGATTAGTTTGAGATAAG+GGG - chr4_1:70229026-70229045 None:intergenic 30.0%
!! CTTCGATTAGTTTGAGATAA+GGG - chr4_1:70229027-70229046 None:intergenic 30.0%
!! ACTTCGATTAGTTTGAGATA+AGG - chr4_1:70229028-70229047 None:intergenic 30.0%
AGTCACAGTAAAGATTCATA+TGG - chr4_1:70229279-70229298 None:intergenic 30.0%
! TTGTGAATTAGATGATTTGC+TGG - chr4_1:70229320-70229339 None:intergenic 30.0%
TTATGAACCAGAAACCATTT+GGG - chr4_1:70229433-70229452 None:intergenic 30.0%
TTTATGAACCAGAAACCATT+TGG - chr4_1:70229434-70229453 None:intergenic 30.0%
TTCAAATTCTACAGTTGCAA+TGG - chr4_1:70229486-70229505 None:intergenic 30.0%
GCAACTGTAGAATTTGAATA+AGG + chr4_1:70229488-70229507 Msa0568600:intron 30.0%
! AAGCTTGTCTTTTGTGTTTA+AGG - chr4_1:70229514-70229533 None:intergenic 30.0%
AACAGTTGGAATAACAATAG+TGG - chr4_1:70229567-70229586 None:intergenic 30.0%
GTTAACGGGAACAAAATTAA+TGG + chr4_1:70229670-70229689 Msa0568600:intron 30.0%
! GAAGATATTGAAGCATTATG+TGG + chr4_1:70229837-70229856 Msa0568600:intron 30.0%
!!! ATTATGGACCCCTTTTTTAA+AGG - chr4_1:70229956-70229975 None:intergenic 30.0%
TTAAAAAAGGGGTCCATAAT+AGG + chr4_1:70229956-70229975 Msa0568600:intron 30.0%
TAAAAAAGGGGTCCATAATA+GGG + chr4_1:70229957-70229976 Msa0568600:intron 30.0%
CCTTATAATGTGAGGTTATT+TGG - chr4_1:70230194-70230213 None:intergenic 30.0%
CCAAATAACCTCACATTATA+AGG + chr4_1:70230191-70230210 Msa0568600:intron 30.0%
CATTTAACTAGCACAAGATT+TGG + chr4_1:70230430-70230449 Msa0568600:intron 30.0%
!!! AAATAAACACGTTTTGAGGA+GGG - chr4_1:70230683-70230702 None:intergenic 30.0%
!! TAACCTTTCAATTTTTGAGG+GGG + chr4_1:70230725-70230744 Msa0568600:intron 30.0%
TCCAAGTTAAAGAACACAAA+GGG - chr4_1:70230844-70230863 None:intergenic 30.0%
ATCCAAGTTAAAGAACACAA+AGG - chr4_1:70230845-70230864 None:intergenic 30.0%
! GAGTTAAGTAATTTGCAAGA+GGG - chr4_1:70230979-70230998 None:intergenic 30.0%
!! CTAGTTGGTGTCTTTTTATA+GGG - chr4_1:70231005-70231024 None:intergenic 30.0%
TCAACAAAAATGCAACAAGA+AGG - chr4_1:70231115-70231134 None:intergenic 30.0%
AACTTGAGAGATACTAAAGA+TGG + chr4_1:70231390-70231409 Msa0568600:CDS 30.0%
TTGTTTAAGCTAAACCATGA+AGG + chr4_1:70231435-70231454 Msa0568600:CDS 30.0%
TGTTTAAGCTAAACCATGAA+GGG + chr4_1:70231436-70231455 Msa0568600:CDS 30.0%
!! AGACTTTAATTATGGATTGG+TGG - chr4_1:70231546-70231565 None:intergenic 30.0%
AGGAGACTTTAATTATGGAT+TGG - chr4_1:70231549-70231568 None:intergenic 30.0%
CTACCAAAAATTGTTCTCTA+AGG - chr4_1:70231591-70231610 None:intergenic 30.0%
! TAAATCACAGATTTTTGTGG+AGG + chr4_1:70231716-70231735 Msa0568600:three_prime_UTR 30.0%
! AGGGGAGCATATATAAATAT+AGG + chr4_1:70231824-70231843 Msa0568600:three_prime_UTR 30.0%
CAAAAACATATGTCTGCATA+AGG - chr4_1:70231916-70231935 None:intergenic 30.0%
TGGCAAAACACTCAATTATC+AGG + chr4_1:70228535-70228554 Msa0568600:five_prime_UTR 35.0%
CATTGATCCGAGTTTAAACT+AGG + chr4_1:70228984-70229003 Msa0568600:intron 35.0%
GTCATATAGCAAAGAACCAT+GGG - chr4_1:70229207-70229226 None:intergenic 35.0%
AGTCATATAGCAAAGAACCA+TGG - chr4_1:70229208-70229227 None:intergenic 35.0%
! TGCTATATGACTTGCAATAG+TGG + chr4_1:70229216-70229235 Msa0568600:intron 35.0%
! GCTATATGACTTGCAATAGT+GGG + chr4_1:70229217-70229236 Msa0568600:intron 35.0%
CACTTCCTTCTCAATTTACA+TGG + chr4_1:70229246-70229265 Msa0568600:intron 35.0%
TCCTTCTCAATTTACATGGT+TGG + chr4_1:70229250-70229269 Msa0568600:intron 35.0%
CACAGTAAAGATTCATATGG+AGG - chr4_1:70229276-70229295 None:intergenic 35.0%
TTTAACATAGCCATCCCAAA+TGG + chr4_1:70229416-70229435 Msa0568600:intron 35.0%
AGCGTGAAAACATTAACAGT+TGG - chr4_1:70229581-70229600 None:intergenic 35.0%
TTTGTTCCCGTTAACAAACT+TGG - chr4_1:70229665-70229684 None:intergenic 35.0%
GAACAAAATTAATGGCTCCA+TGG + chr4_1:70229678-70229697 Msa0568600:intron 35.0%
TCTGCACTCTGATATACATT+TGG - chr4_1:70229887-70229906 None:intergenic 35.0%
GAGTAATTACCTGTTATTGC+AGG + chr4_1:70229905-70229924 Msa0568600:intron 35.0%
! TTATTGCAGGTCATTTGCTA+AGG + chr4_1:70229918-70229937 Msa0568600:intron 35.0%
! ATGGGTGGACCTTTAAAAAA+GGG + chr4_1:70229944-70229963 Msa0568600:intron 35.0%
AAAAAAGGGGTCCATAATAG+GGG + chr4_1:70229958-70229977 Msa0568600:intron 35.0%
CAAAAGCTCCTTATAATGTG+AGG - chr4_1:70230202-70230221 None:intergenic 35.0%
ACAAGATTTGGATTTGTGCA+TGG + chr4_1:70230442-70230461 Msa0568600:intron 35.0%
! TGACTTGTGAGATTGGAAAA+GGG + chr4_1:70230607-70230626 Msa0568600:intron 35.0%
GAATCACATATCACCCTTAT+AGG + chr4_1:70230629-70230648 Msa0568600:intron 35.0%
!!! AATAAACACGTTTTGAGGAG+GGG - chr4_1:70230682-70230701 None:intergenic 35.0%
!!! CAAATAAACACGTTTTGAGG+AGG - chr4_1:70230684-70230703 None:intergenic 35.0%
ACCCTTTGTGTTCTTTAACT+TGG + chr4_1:70230840-70230859 Msa0568600:five_prime_UTR 35.0%
!! ACACTTTTTGCTTCGATTAC+AGG - chr4_1:70230868-70230887 None:intergenic 35.0%
! GGAGTTAAGTAATTTGCAAG+AGG - chr4_1:70230980-70230999 None:intergenic 35.0%
!! TTGGTGTCTTTTTATAGGGA+GGG - chr4_1:70231001-70231020 None:intergenic 35.0%
!! CCTAGTTGGTGTCTTTTTAT+AGG - chr4_1:70231006-70231025 None:intergenic 35.0%
CCTATAAAAAGACACCAACT+AGG + chr4_1:70231003-70231022 Msa0568600:five_prime_UTR 35.0%
TTCATCTTAACTACAGCTCA+AGG + chr4_1:70231140-70231159 Msa0568600:CDS 35.0%
TACTAAAGATGGAGCTGAAA+AGG + chr4_1:70231401-70231420 Msa0568600:CDS 35.0%
ACTAAAGATGGAGCTGAAAA+GGG + chr4_1:70231402-70231421 Msa0568600:CDS 35.0%
!! TGATGACTTGTTGGATACAA+TGG + chr4_1:70231497-70231516 Msa0568600:CDS 35.0%
AGCTCATAGATAGAATTGCA+GGG - chr4_1:70231648-70231667 None:intergenic 35.0%
AAGCTCATAGATAGAATTGC+AGG - chr4_1:70231649-70231668 None:intergenic 35.0%
TGATTAAGCATCAACAACAC+TGG - chr4_1:70231750-70231769 None:intergenic 35.0%
ACATATGTCTGCATAAGGTA+AGG - chr4_1:70231911-70231930 None:intergenic 35.0%
ATAACTCTCCTCCATGCATA+TGG - chr4_1:70228503-70228522 None:intergenic 40.0%
ATATAGCAAAGAACCATGGG+TGG - chr4_1:70229204-70229223 None:intergenic 40.0%
GCCAACCATGTAAATTGAGA+AGG - chr4_1:70229254-70229273 None:intergenic 40.0%
GAATCTCAGTTCGGCTTAAA+GGG - chr4_1:70229464-70229483 None:intergenic 40.0%
GTTGCAATGGAATCTCAGTT+CGG - chr4_1:70229473-70229492 None:intergenic 40.0%
!! CCTGAAGCCAAGTTTGTTAA+CGG + chr4_1:70229655-70229674 Msa0568600:intron 40.0%
!! CTGAAGCCAAGTTTGTTAAC+GGG + chr4_1:70229656-70229675 Msa0568600:intron 40.0%
AATTAATGGCTCCATGGATC+TGG + chr4_1:70229684-70229703 Msa0568600:intron 40.0%
ATTAATGGCTCCATGGATCT+GGG + chr4_1:70229685-70229704 Msa0568600:intron 40.0%
GCAGAGAAAACGTCATGAAT+CGG + chr4_1:70229720-70229739 Msa0568600:intron 40.0%
AGCAAATGACCTGCAATAAC+AGG - chr4_1:70229917-70229936 None:intergenic 40.0%
!!! CTTTTTTAAAGGTCCACCCA+TGG - chr4_1:70229945-70229964 None:intergenic 40.0%
! CATGGGTGGACCTTTAAAAA+AGG + chr4_1:70229943-70229962 Msa0568600:intron 40.0%
! TGGGTGGACCTTTAAAAAAG+GGG + chr4_1:70229945-70229964 Msa0568600:intron 40.0%
TATCTAGGAAGCCCCTATTA+TGG - chr4_1:70229972-70229991 None:intergenic 40.0%
ATAGGGGCTTCCTAGATAAT+TGG + chr4_1:70229974-70229993 Msa0568600:intron 40.0%
GTCTATGTGCACGATTATCA+CGG + chr4_1:70230476-70230495 Msa0568600:intron 40.0%
ATCTCACAAGTCACAACACA+TGG - chr4_1:70230600-70230619 None:intergenic 40.0%
! TGTGTTGTGACTTGTGAGAT+TGG + chr4_1:70230600-70230619 Msa0568600:intron 40.0%
! GTGACTTGTGAGATTGGAAA+AGG + chr4_1:70230606-70230625 Msa0568600:intron 40.0%
TGCATACAGGAGACCTATAA+GGG - chr4_1:70230645-70230664 None:intergenic 40.0%
TTGCATACAGGAGACCTATA+AGG - chr4_1:70230646-70230665 None:intergenic 40.0%
TTATAGGTCTCCTGTATGCA+AGG + chr4_1:70230645-70230664 Msa0568600:intron 40.0%
!!! ATAAACACGTTTTGAGGAGG+GGG - chr4_1:70230681-70230700 None:intergenic 40.0%
ACACCCCCTCAAAAATTGAA+AGG - chr4_1:70230731-70230750 None:intergenic 40.0%
TTTGTGTCCCTCGAAATTGT+TGG - chr4_1:70230790-70230809 None:intergenic 40.0%
AAGCCAACAACGTAACATTG+AGG + chr4_1:70230810-70230829 Msa0568600:five_prime_UTR 40.0%
AGCCAACAACGTAACATTGA+GGG + chr4_1:70230811-70230830 Msa0568600:five_prime_UTR 40.0%
TTCCCTCAATGTTACGTTGT+TGG - chr4_1:70230816-70230835 None:intergenic 40.0%
ACAACGTAACATTGAGGGAA+TGG + chr4_1:70230816-70230835 Msa0568600:five_prime_UTR 40.0%
!!! AAGTGTTGTCATTTTCAGCG+AGG + chr4_1:70230882-70230901 Msa0568600:five_prime_UTR 40.0%
!! GTTGGTGTCTTTTTATAGGG+AGG - chr4_1:70231002-70231021 None:intergenic 40.0%
TGCATAAGATATGGCCTAGT+TGG - chr4_1:70231020-70231039 None:intergenic 40.0%
ATTAATGAACGATGCAGCAG+TGG - chr4_1:70231199-70231218 None:intergenic 40.0%
!! ATGGATTGGTGGATTCTTTG+AGG - chr4_1:70231535-70231554 None:intergenic 40.0%
! GGTAATTAAGCACTGTGGAT+TGG - chr4_1:70231627-70231646 None:intergenic 40.0%
! TTGGAAGTTATGAGAGTGTG+AGG + chr4_1:70231804-70231823 Msa0568600:three_prime_UTR 40.0%
! TGGAAGTTATGAGAGTGTGA+GGG + chr4_1:70231805-70231824 Msa0568600:three_prime_UTR 40.0%
TGGGTGGACCCTAAAAAAAG+AGG - chr4_1:70229188-70229207 None:intergenic 45.0%
!!! CTTTTTTTAGGGTCCACCCA+TGG + chr4_1:70229188-70229207 Msa0568600:intron 45.0%
TAGCCATCCCAAATGGTTTC+TGG + chr4_1:70229423-70229442 Msa0568600:intron 45.0%
GAACCAGAAACCATTTGGGA+TGG - chr4_1:70229429-70229448 None:intergenic 45.0%
AGTTCGGCTTAAAGGGAATG+AGG - chr4_1:70229457-70229476 None:intergenic 45.0%
GGAATCTCAGTTCGGCTTAA+AGG - chr4_1:70229465-70229484 None:intergenic 45.0%
CCGTTAACAAACTTGGCTTC+AGG - chr4_1:70229658-70229677 None:intergenic 45.0%
GAGAAAACGTCATGAATCGG+TGG + chr4_1:70229723-70229742 Msa0568600:intron 45.0%
ATCGGTGGTTTCAGAATCAG+CGG + chr4_1:70229738-70229757 Msa0568600:intron 45.0%
! AGGTCATTTGCTAAGGACCA+TGG + chr4_1:70229925-70229944 Msa0568600:intron 45.0%
! GGTCATTTGCTAAGGACCAT+GGG + chr4_1:70229926-70229945 Msa0568600:intron 45.0%
TATGTGCACGATTATCACGG+TGG + chr4_1:70230479-70230498 Msa0568600:intron 45.0%
!!! TAAACACGTTTTGAGGAGGG+GGG - chr4_1:70230680-70230699 None:intergenic 45.0%
CACAGTGCCAACAATTTCGA+GGG + chr4_1:70230780-70230799 Msa0568600:five_prime_UTR 45.0%
CTTGTGTTTGTGTCCCTTCA+TGG - chr4_1:70231452-70231471 None:intergenic 45.0%
!! ATTGGTGGATTCTTTGAGGC+AGG - chr4_1:70231531-70231550 None:intergenic 45.0%
! TGCAGGGTAATTAAGCACTG+TGG - chr4_1:70231632-70231651 None:intergenic 45.0%
! GGAAGTTATGAGAGTGTGAG+GGG + chr4_1:70231806-70231825 Msa0568600:three_prime_UTR 45.0%
!! CTATTATTTAAAAAAATATA+TGG - chr4_1:70228933-70228952 None:intergenic 5.0%
!!! AAAAATTAAGATTTTTATTT+TGG + chr4_1:70229125-70229144 Msa0568600:intron 5.0%
AACATCCCATCCCAGATCCA+TGG - chr4_1:70229698-70229717 None:intergenic 50.0%
TTTCAGAATCAGCGGTGCGA+CGG + chr4_1:70229746-70229765 Msa0568600:intron 50.0%
! CATTTGCTAAGGACCATGGG+TGG + chr4_1:70229929-70229948 Msa0568600:intron 50.0%
GGTCTCCTGTATGCAAGGAA+TGG + chr4_1:70230650-70230669 Msa0568600:intron 50.0%
!!! AAACACGTTTTGAGGAGGGG+GGG - chr4_1:70230679-70230698 None:intergenic 50.0%
GCACAGTGCCAACAATTTCG+AGG + chr4_1:70230779-70230798 Msa0568600:five_prime_UTR 50.0%
! ATTTTCAGCGAGGACAGAGC+AGG + chr4_1:70230892-70230911 Msa0568600:five_prime_UTR 50.0%
!! GCAACAAGAAGGCGTTTGCT+TGG - chr4_1:70231104-70231123 None:intergenic 50.0%
ATGAACGATGCAGCAGTGGT+AGG - chr4_1:70231195-70231214 None:intergenic 50.0%
ATGGCTCCATGGATCTGGGA+TGG + chr4_1:70229689-70229708 Msa0568600:intron 55.0%
! TGGCTCCATGGATCTGGGAT+GGG + chr4_1:70229690-70229709 Msa0568600:intron 55.0%
GGGCTCCATTCCTTGCATAC+AGG - chr4_1:70230658-70230677 None:intergenic 55.0%
!!! AACACGTTTTGAGGAGGGGG+GGG - chr4_1:70230678-70230697 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 70228499 70232009 70228499 ID=Msa0568600;Name=Msa0568600
chr4_1 mRNA 70228499 70232009 70228499 ID=Msa0568600-mRNA-1;Parent=Msa0568600;Name=Msa0568600-mRNA-1;_AED=0.02;_eAED=0.02;_QI=381|1|1|1|0.5|0.33|3|449|82
chr4_1 exon 70228499 70228556 70228499 ID=Msa0568600-mRNA-1:exon:23620;Parent=Msa0568600-mRNA-1
chr4_1 exon 70230766 70231161 70230766 ID=Msa0568600-mRNA-1:exon:23621;Parent=Msa0568600-mRNA-1
chr4_1 exon 70231385 70232009 70231385 ID=Msa0568600-mRNA-1:exon:23622;Parent=Msa0568600-mRNA-1
chr4_1 five_prime_UTR 70228499 70228556 70228499 ID=Msa0568600-mRNA-1:five_prime_utr;Parent=Msa0568600-mRNA-1
chr4_1 five_prime_UTR 70230766 70231088 70230766 ID=Msa0568600-mRNA-1:five_prime_utr;Parent=Msa0568600-mRNA-1
chr4_1 CDS 70231089 70231161 70231089 ID=Msa0568600-mRNA-1:cds;Parent=Msa0568600-mRNA-1
chr4_1 CDS 70231385 70231560 70231385 ID=Msa0568600-mRNA-1:cds;Parent=Msa0568600-mRNA-1
chr4_1 three_prime_UTR 70231561 70232009 70231561 ID=Msa0568600-mRNA-1:three_prime_utr;Parent=Msa0568600-mRNA-1
Gene Sequence

>Msa0568600

ATGGCTATTGTACCAAGCAAACGCCTTCTTGTTGCATTTTTGTTGATTTGCTTCATCTTAACTACAGCTCAAGCTAGAAACTTGAGAGATACTAAAGATGGAGCTGAAAAGGGTCAAAGTAGCTTGTTTAAGCTAAACCATGAAGGGACACAAACACAAGAAAAAACACAAACACAAGAAAAAAATGATGACTTGTTGGATACAATGGACTACACACCTGCCTCAAAGAATCCACCAATCCATAATTAA
Protein sequence

>Msa0568600

MAIVPSKRLLVAFLLICFILTTAQARNLRDTKDGAEKGQSSLFKLNHEGTQTQEKTQTQEKNDDLLDTMDYTPASKNPPIHN*