AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1361200


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1361200 MtrunA17_Chr8g0383471 93.902 82 5 0 1 82 1 82 2.01e-44 137
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 46 sgRNAs with CRISPR-Local

Find 133 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAACATTGATTATGATTTA+TGG 0.145840 tig0017374:+54153 None:intergenic
AACTATAGATATTTGATATA+TGG 0.209688 tig0017374:+55084 None:intergenic
AAACTAGGAGACTTTAATTA+TGG 0.215662 tig0017374:+54582 None:intergenic
CTTGTGTTTGTGTCCCTTCA+TGG 0.225941 tig0017374:+54684 None:intergenic
TTCCCTCAATGTTACGTTGT+TGG 0.341086 tig0017374:+55320 None:intergenic
TGGCAAAACACTCAATTATC+AGG 0.352740 tig0017374:-56763 Msa1361200:intron
GATTTATGGATGTAGATATA+TGG 0.352860 tig0017374:+54167 None:intergenic
ACCCTTTGTGTTCTTTAACT+TGG 0.379775 tig0017374:-55293 Msa1361200:five_prime_UTR
TACTAAAGATGGAGCTGAAA+AGG 0.386508 tig0017374:-54732 Msa1361200:CDS
AGGGGAGCATATATAAATAT+AGG 0.412997 tig0017374:-54308 Msa1361200:three_prime_UTR
TTGAGAGTAATATTAAGATA+TGG 0.450623 tig0017374:-55063 Msa1361200:exon
TGCATAAGATATGGCCTAGT+TGG 0.452766 tig0017374:+55116 None:intergenic
ATAACTCTCCTCCATGCATA+TGG 0.467549 tig0017374:+56798 None:intergenic
AAGCTCATAGATAGAATTGC+AGG 0.467654 tig0017374:+54486 None:intergenic
TTTGTGTCCCTCGAAATTGT+TGG 0.468411 tig0017374:+55346 None:intergenic
AGGAGACTTTAATTATGGAT+TGG 0.479268 tig0017374:+54587 None:intergenic
AGACTTTAATTATGGATTGG+TGG 0.484159 tig0017374:+54590 None:intergenic
ATGGATTGGTGGATTCTTTG+AGG 0.484420 tig0017374:+54601 None:intergenic
GCAACAAGAAGGCGTTTGCT+TGG 0.499691 tig0017374:+55032 None:intergenic
ATTGGTGGATTCTTTGAGGC+AGG 0.510445 tig0017374:+54605 None:intergenic
GGTAATTAAGCACTGTGGAT+TGG 0.514972 tig0017374:+54508 None:intergenic
ACTAAAGATGGAGCTGAAAA+GGG 0.528078 tig0017374:-54731 Msa1361200:CDS
AGCCAACAACGTAACATTGA+GGG 0.530525 tig0017374:-55322 Msa1361200:five_prime_UTR
TGGAAGTTATGAGAGTGTGA+GGG 0.536134 tig0017374:-54327 Msa1361200:three_prime_UTR
TTGGAAGTTATGAGAGTGTG+AGG 0.537291 tig0017374:-54328 Msa1361200:three_prime_UTR
TCCAAGTTAAAGAACACAAA+GGG 0.541459 tig0017374:+55292 None:intergenic
GGAAGTTATGAGAGTGTGAG+GGG 0.541779 tig0017374:-54326 Msa1361200:three_prime_UTR
ACATATGTCTGCATAAGGTA+AGG 0.543962 tig0017374:+54224 None:intergenic
TTGTTTAAGCTAAACCATGA+AGG 0.545024 tig0017374:-54698 Msa1361200:CDS
GGAGTTAAGTAATTTGCAAG+AGG 0.553059 tig0017374:+55156 None:intergenic
TGATGACTTGTTGGATACAA+TGG 0.554869 tig0017374:-54636 Msa1361200:CDS
ACAACGTAACATTGAGGGAA+TGG 0.556781 tig0017374:-55317 Msa1361200:five_prime_UTR
AACTTGAGAGATACTAAAGA+TGG 0.564007 tig0017374:-54743 Msa1361200:CDS
GAGTTAAGTAATTTGCAAGA+GGG 0.566314 tig0017374:+55157 None:intergenic
TTCATCTTAACTACAGCTCA+AGG 0.570896 tig0017374:-54993 Msa1361200:intron
GCACAGTGCCAACAATTTCG+AGG 0.572541 tig0017374:-55354 Msa1361200:five_prime_UTR
TGTTTAAGCTAAACCATGAA+GGG 0.579354 tig0017374:-54697 Msa1361200:CDS
AGAGTTATTCTATCAAGTCA+TGG 0.596916 tig0017374:-56783 Msa1361200:five_prime_UTR
CACAGTGCCAACAATTTCGA+GGG 0.621204 tig0017374:-55353 Msa1361200:five_prime_UTR
AAGCCAACAACGTAACATTG+AGG 0.634993 tig0017374:-55323 Msa1361200:five_prime_UTR
ATCCAAGTTAAAGAACACAA+AGG 0.639483 tig0017374:+55291 None:intergenic
TTGGGGAAGAGCCATATGCA+TGG 0.644947 tig0017374:-56809 None:intergenic
TGATTAAGCATCAACAACAC+TGG 0.649291 tig0017374:+54385 None:intergenic
AGCTCATAGATAGAATTGCA+GGG 0.664206 tig0017374:+54487 None:intergenic
TGCAGGGTAATTAAGCACTG+TGG 0.694712 tig0017374:+54503 None:intergenic
GGGAAGAGCCATATGCATGG+AGG 0.733072 tig0017374:-56806 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGCTATAATAAAAAATTAT+AGG + tig0017374:54400-54419 None:intergenic 10.0%
!!! TTTTTATAGATTTTTTTTTG+AGG - tig0017374:54519-54538 Msa1361200:three_prime_UTR 10.0%
!!! AATTTTTTATAATCTATAAG+AGG + tig0017374:54859-54878 None:intergenic 10.0%
!! AATTAATATTTGTATATTTG+AGG - tig0017374:56134-56153 Msa1361200:intron 10.0%
!! TAAAAAAATATGTATTGTGA+TGG - tig0017374:54274-54293 Msa1361200:three_prime_UTR 15.0%
!!! TTTTCTTATATCAGATATAT+AGG - tig0017374:54350-54369 Msa1361200:three_prime_UTR 15.0%
!!! ATTAGAATTTTGATTCATTT+CGG - tig0017374:54743-54762 Msa1361200:CDS 15.0%
!!! TACAATTTTAAAAATACTGA+GGG + tig0017374:55052-55071 None:intergenic 15.0%
!!! TTACAATTTTAAAAATACTG+AGG + tig0017374:55053-55072 None:intergenic 15.0%
!!! AAACATTATCATTGATTTTT+CGG + tig0017374:55355-55374 None:intergenic 15.0%
!!! ATTTTATTAATGCAGATATT+TGG - tig0017374:55562-55581 Msa1361200:intron 15.0%
!! AACTATAGATATTTGATATA+TGG + tig0017374:55863-55882 None:intergenic 15.0%
!! GCAAAATAGTTTAGTAATAT+TGG - tig0017374:54416-54435 Msa1361200:three_prime_UTR 20.0%
!!! AGTTTTAAAAATCTCTTGTT+CGG + tig0017374:54698-54717 None:intergenic 20.0%
!! GGATGTTATATATAGATAAT+TGG - tig0017374:54792-54811 Msa1361200:intron 20.0%
!!! TTTTAACCTTTCAATTTTTG+AGG - tig0017374:55533-55552 Msa1361200:intron 20.0%
!!! TTTAACCTTTCAATTTTTGA+GGG - tig0017374:55534-55553 Msa1361200:intron 20.0%
!!! AAAACAATTGTTGTGATTTT+AGG + tig0017374:55744-55763 None:intergenic 20.0%
!! TTGAGAGTAATATTAAGATA+TGG - tig0017374:55881-55900 Msa1361200:intron 20.0%
!! GAAAACATCACATAAATTAT+AGG + tig0017374:56160-56179 None:intergenic 20.0%
!!! ATTTATGTGATGTTTTCTTT+TGG - tig0017374:56163-56182 Msa1361200:intron 20.0%
!! GAAAGAGAAAAAATAAAACT+AGG + tig0017374:56380-56399 None:intergenic 20.0%
!!! ATTTTTGGTAGGAAAAAAAA+AGG - tig0017374:56411-56430 Msa1361200:intron 20.0%
!!! ATATTTTGAACTTTGAAGAT+AGG - tig0017374:56493-56512 Msa1361200:intron 20.0%
!!! TATTTTGAACTTTGAAGATA+GGG - tig0017374:56494-56513 Msa1361200:intron 20.0%
!!! CACTTTTAAATGTATAGTTT+TGG - tig0017374:56597-56616 Msa1361200:intron 20.0%
! GTATATCTCTTCTTTATTCA+TGG + tig0017374:54327-54346 None:intergenic 25.0%
! TATAGGAAACTTTCAAAACT+TGG + tig0017374:54383-54402 None:intergenic 25.0%
! TGAAAATCCTAGTTTAAACT+CGG + tig0017374:54640-54659 None:intergenic 25.0%
!!! GTTTTAAAAATCTCTTGTTC+GGG + tig0017374:54697-54716 None:intergenic 25.0%
!!! GAAATTTAGATTTTGACACA+AGG - tig0017374:55163-55182 Msa1361200:five_prime_UTR 25.0%
! TATCATAGTATATGTATCGA+TGG + tig0017374:55321-55340 None:intergenic 25.0%
! TTTGTCAGAATATATATGCA+AGG - tig0017374:55372-55391 Msa1361200:intron 25.0%
!! AAACAAATAAACACGTTTTG+AGG + tig0017374:55498-55517 None:intergenic 25.0%
!!! TTAACCTTTCAATTTTTGAG+GGG - tig0017374:55535-55554 Msa1361200:intron 25.0%
!!! TTTTGAAGTTGCATAAGATA+TGG + tig0017374:55840-55859 None:intergenic 25.0%
!! TGCATCGTTCATTAATTTTA+AGG - tig0017374:56014-56033 Msa1361200:intron 25.0%
!! AGAAAAAAATGATGACTTGT+TGG - tig0017374:56299-56318 Msa1361200:intron 25.0%
! AAACTAGGAGACTTTAATTA+TGG + tig0017374:56365-56384 None:intergenic 25.0%
!! TTTCCTTAGAGAACAATTTT+TGG - tig0017374:56396-56415 Msa1361200:intron 25.0%
! AAAAATCTGTGATTTAAGTG+TGG + tig0017374:56524-56543 None:intergenic 25.0%
!! ACTTAAATCACAGATTTTTG+TGG - tig0017374:56525-56544 Msa1361200:intron 25.0%
!! ATATACTAAGCATCTGTTTT+TGG + tig0017374:56697-56716 None:intergenic 25.0%
! GATTTATGGATGTAGATATA+TGG + tig0017374:56780-56799 None:intergenic 25.0%
!! GGAACATTGATTATGATTTA+TGG + tig0017374:56794-56813 None:intergenic 25.0%
AGAGTTATTCTATCAAGTCA+TGG - tig0017374:54161-54180 Msa1361200:three_prime_UTR 30.0%
AGTAGATATTTGTACTGTGA+TGG - tig0017374:54241-54260 Msa1361200:three_prime_UTR 30.0%
TGATATACGTAGCATATGAT+AGG - tig0017374:54448-54467 Msa1361200:three_prime_UTR 30.0%
!! TTCGATTAGTTTGAGATAAG+GGG + tig0017374:54672-54691 None:intergenic 30.0%
!! CTTCGATTAGTTTGAGATAA+GGG + tig0017374:54673-54692 None:intergenic 30.0%
!! ACTTCGATTAGTTTGAGATA+AGG + tig0017374:54674-54693 None:intergenic 30.0%
CCTTATAATGTGAGGTTATT+TGG + tig0017374:55006-55025 None:intergenic 30.0%
CCAAATAACCTCACATTATA+AGG - tig0017374:55003-55022 Msa1361200:CDS 30.0%
CATTTAACTAGCACAAGATT+TGG - tig0017374:55242-55261 Msa1361200:five_prime_UTR 30.0%
!!! AAATAAACACGTTTTGAGGA+GGG + tig0017374:55494-55513 None:intergenic 30.0%
!! TAACCTTTCAATTTTTGAGG+GGG - tig0017374:55536-55555 Msa1361200:intron 30.0%
TCCAAGTTAAAGAACACAAA+GGG + tig0017374:55655-55674 None:intergenic 30.0%
ATCCAAGTTAAAGAACACAA+AGG + tig0017374:55656-55675 None:intergenic 30.0%
! GAGTTAAGTAATTTGCAAGA+GGG + tig0017374:55790-55809 None:intergenic 30.0%
!! CTAGTTGGTGTCTTTTTATA+GGG + tig0017374:55816-55835 None:intergenic 30.0%
TCAACAAAAATGCAACAAGA+AGG + tig0017374:55926-55945 None:intergenic 30.0%
AACTTGAGAGATACTAAAGA+TGG - tig0017374:56201-56220 Msa1361200:intron 30.0%
TTGTTTAAGCTAAACCATGA+AGG - tig0017374:56246-56265 Msa1361200:intron 30.0%
TGTTTAAGCTAAACCATGAA+GGG - tig0017374:56247-56266 Msa1361200:intron 30.0%
!! AGACTTTAATTATGGATTGG+TGG + tig0017374:56357-56376 None:intergenic 30.0%
AGGAGACTTTAATTATGGAT+TGG + tig0017374:56360-56379 None:intergenic 30.0%
CTACCAAAAATTGTTCTCTA+AGG + tig0017374:56402-56421 None:intergenic 30.0%
!!! CTTAGAGAACAATTTTTGGT+AGG - tig0017374:56400-56419 Msa1361200:intron 30.0%
! TAAATCACAGATTTTTGTGG+AGG - tig0017374:56528-56547 Msa1361200:intron 30.0%
! AGGGGAGCATATATAAATAT+AGG - tig0017374:56636-56655 Msa1361200:intron 30.0%
CAAAAACATATGTCTGCATA+AGG + tig0017374:56728-56747 None:intergenic 30.0%
TGGCAAAACACTCAATTATC+AGG - tig0017374:54181-54200 Msa1361200:three_prime_UTR 35.0%
CATTGATCCGAGTTTAAACT+AGG - tig0017374:54630-54649 Msa1361200:CDS 35.0%
CAAAAGCTCCTTATAATGTG+AGG + tig0017374:55014-55033 None:intergenic 35.0%
ACAAGATTTGGATTTGTGCA+TGG - tig0017374:55254-55273 Msa1361200:five_prime_UTR 35.0%
GAATCACATATCACCCTTAT+AGG - tig0017374:55441-55460 Msa1361200:intron 35.0%
!!! AATAAACACGTTTTGAGGAG+GGG + tig0017374:55493-55512 None:intergenic 35.0%
!!! CAAATAAACACGTTTTGAGG+AGG + tig0017374:55495-55514 None:intergenic 35.0%
ACCCTTTGTGTTCTTTAACT+TGG - tig0017374:55651-55670 Msa1361200:intron 35.0%
!! ACACTTTTTGCTTCGATTAC+AGG + tig0017374:55679-55698 None:intergenic 35.0%
! GGAGTTAAGTAATTTGCAAG+AGG + tig0017374:55791-55810 None:intergenic 35.0%
!! TTGGTGTCTTTTTATAGGGA+GGG + tig0017374:55812-55831 None:intergenic 35.0%
!! CCTAGTTGGTGTCTTTTTAT+AGG + tig0017374:55817-55836 None:intergenic 35.0%
CCTATAAAAAGACACCAACT+AGG - tig0017374:55814-55833 Msa1361200:intron 35.0%
TTCATCTTAACTACAGCTCA+AGG - tig0017374:55951-55970 Msa1361200:intron 35.0%
TACTAAAGATGGAGCTGAAA+AGG - tig0017374:56212-56231 Msa1361200:intron 35.0%
ACTAAAGATGGAGCTGAAAA+GGG - tig0017374:56213-56232 Msa1361200:intron 35.0%
!! TGATGACTTGTTGGATACAA+TGG - tig0017374:56308-56327 Msa1361200:intron 35.0%
AGCTCATAGATAGAATTGCA+GGG + tig0017374:56460-56479 None:intergenic 35.0%
AAGCTCATAGATAGAATTGC+AGG + tig0017374:56461-56480 None:intergenic 35.0%
TGATTAAGCATCAACAACAC+TGG + tig0017374:56562-56581 None:intergenic 35.0%
ACATATGTCTGCATAAGGTA+AGG + tig0017374:56723-56742 None:intergenic 35.0%
ATAACTCTCCTCCATGCATA+TGG + tig0017374:54149-54168 None:intergenic 40.0%
GTCTATGTGCACGATTATCA+CGG - tig0017374:55288-55307 Msa1361200:five_prime_UTR 40.0%
TGACCTGTGAGATTGGAAAA+GGG - tig0017374:55419-55438 Msa1361200:intron 40.0%
! ATTCCCTTTTCCAATCTCAC+AGG + tig0017374:55425-55444 None:intergenic 40.0%
TGCATACAGGAGACCTATAA+GGG + tig0017374:55457-55476 None:intergenic 40.0%
TTGCATACAGGAGACCTATA+AGG + tig0017374:55458-55477 None:intergenic 40.0%
TTATAGGTCTCCTGTATGCA+AGG - tig0017374:55457-55476 Msa1361200:intron 40.0%
!!! ATAAACACGTTTTGAGGAGG+GGG + tig0017374:55492-55511 None:intergenic 40.0%
ACACCCCCTCAAAAATTGAA+AGG + tig0017374:55542-55561 None:intergenic 40.0%
TTTGTGTCCCTCGAAATTGT+TGG + tig0017374:55601-55620 None:intergenic 40.0%
AAGCCAACAACGTAACATTG+AGG - tig0017374:55621-55640 Msa1361200:intron 40.0%
AGCCAACAACGTAACATTGA+GGG - tig0017374:55622-55641 Msa1361200:intron 40.0%
TTCCCTCAATGTTACGTTGT+TGG + tig0017374:55627-55646 None:intergenic 40.0%
ACAACGTAACATTGAGGGAA+TGG - tig0017374:55627-55646 Msa1361200:intron 40.0%
!!! AAGTGTTGTCATTTTCAGCG+AGG - tig0017374:55693-55712 Msa1361200:intron 40.0%
!! GTTGGTGTCTTTTTATAGGG+AGG + tig0017374:55813-55832 None:intergenic 40.0%
TGCATAAGATATGGCCTAGT+TGG + tig0017374:55831-55850 None:intergenic 40.0%
ATTAATGAACGATGCAGCAG+TGG + tig0017374:56010-56029 None:intergenic 40.0%
!! ATGGATTGGTGGATTCTTTG+AGG + tig0017374:56346-56365 None:intergenic 40.0%
! GGTAATTAAGCACTGTGGAT+TGG + tig0017374:56439-56458 None:intergenic 40.0%
! TTGGAAGTTATGAGAGTGTG+AGG - tig0017374:56616-56635 Msa1361200:intron 40.0%
! TGGAAGTTATGAGAGTGTGA+GGG - tig0017374:56617-56636 Msa1361200:intron 40.0%
TATGTGCACGATTATCACGG+TGG - tig0017374:55291-55310 Msa1361200:five_prime_UTR 45.0%
ATCTCACAGGTCACAACACA+TGG + tig0017374:55412-55431 None:intergenic 45.0%
TGTGTTGTGACCTGTGAGAT+TGG - tig0017374:55412-55431 Msa1361200:intron 45.0%
GTGACCTGTGAGATTGGAAA+AGG - tig0017374:55418-55437 Msa1361200:intron 45.0%
!!! TAAACACGTTTTGAGGAGGG+GGG + tig0017374:55491-55510 None:intergenic 45.0%
CACAGTGCCAACAATTTCGA+GGG - tig0017374:55591-55610 Msa1361200:intron 45.0%
CTTGTGTTTGTGTCCCTTCA+TGG + tig0017374:56263-56282 None:intergenic 45.0%
!! ATTGGTGGATTCTTTGAGGC+AGG + tig0017374:56342-56361 None:intergenic 45.0%
! TGCAGGGTAATTAAGCACTG+TGG + tig0017374:56444-56463 None:intergenic 45.0%
! GGAAGTTATGAGAGTGTGAG+GGG - tig0017374:56618-56637 Msa1361200:intron 45.0%
!! CTATTATTTAAAAAAATATA+TGG + tig0017374:54579-54598 None:intergenic 5.0%
!!! AAAAATTAAGATTTTTATTT+TGG - tig0017374:54771-54790 Msa1361200:intron 5.0%
GGTCTCCTGTATGCAAGGAA+TGG - tig0017374:55462-55481 Msa1361200:intron 50.0%
!!! AAACACGTTTTGAGGAGGGG+GGG + tig0017374:55490-55509 None:intergenic 50.0%
GCACAGTGCCAACAATTTCG+AGG - tig0017374:55590-55609 Msa1361200:intron 50.0%
! ATTTTCAGCGAGGACAGAGC+AGG - tig0017374:55703-55722 Msa1361200:intron 50.0%
!! GCAACAAGAAGGCGTTTGCT+TGG + tig0017374:55915-55934 None:intergenic 50.0%
ATGAACGATGCAGCAGTGGT+AGG + tig0017374:56006-56025 None:intergenic 50.0%
GGGCTCCATTCCTTGCATAC+AGG + tig0017374:55470-55489 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
tig0017374 gene 54145 56821 54145 ID=Msa1361200;Name=Msa1361200
tig0017374 mRNA 54145 56821 54145 ID=Msa1361200-mRNA-1;Parent=Msa1361200;Name=Msa1361200-mRNA-1;_AED=0.02;_eAED=0.02;_QI=381|1|1|1|0.5|0.33|3|450|82
tig0017374 exon 54145 54770 54145 ID=Msa1361200-mRNA-1:exon:12298;Parent=Msa1361200-mRNA-1
tig0017374 exon 54994 55389 54994 ID=Msa1361200-mRNA-1:exon:12297;Parent=Msa1361200-mRNA-1
tig0017374 exon 56764 56821 56764 ID=Msa1361200-mRNA-1:exon:12296;Parent=Msa1361200-mRNA-1
tig0017374 five_prime_UTR 56764 56821 56764 ID=Msa1361200-mRNA-1:five_prime_utr;Parent=Msa1361200-mRNA-1
tig0017374 five_prime_UTR 55067 55389 55067 ID=Msa1361200-mRNA-1:five_prime_utr;Parent=Msa1361200-mRNA-1
tig0017374 CDS 54994 55066 54994 ID=Msa1361200-mRNA-1:cds;Parent=Msa1361200-mRNA-1
tig0017374 CDS 54595 54770 54595 ID=Msa1361200-mRNA-1:cds;Parent=Msa1361200-mRNA-1
tig0017374 three_prime_UTR 54145 54594 54145 ID=Msa1361200-mRNA-1:three_prime_utr;Parent=Msa1361200-mRNA-1
Gene Sequence

>Msa1361200

ATGGCTATTGTACCAAGCAAACGCCTTCTTGTTGCATTTTTGTTGATTTGCTTCATCTTAACTACAGCTCAAGCTAGAAACTTGAGAGATACTAAAGATGGAGCTGAAAAGGGTCAAAGTAGCTTGTTTAAGCTAAACCATGAAGGGACACAAACACAAGAAAAAACACAAACACAAGAAAAAAATGATGACTTGTTGGATACAATGGACTACACACCTGCCTCAAAGAATCCACCAATCCATAATTAA

Protein sequence

>Msa1361200

MAIVPSKRLLVAFLLICFILTTAQARNLRDTKDGAEKGQSSLFKLNHEGTQTQEKTQTQEKNDDLLDTMDYTPASKNPPIHN*