AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0646650

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0646650 MtrunA17_Chr2g0305551 70.950 179 13 1 1 140 1 179 2.59e-81 237
Msa0646650 MtrunA17_Chr4g0034311 72.159 176 10 1 1 137 1 176 2.87e-77 233
Msa0646650 MtrunA17_Chr4g0034311 63.158 171 18 4 1 131 177 342 1.80e-60 189
Msa0646650 MtrunA17_Chr2g0305541 61.404 171 21 4 1 131 1 166 7.56e-61 185
Msa0646650 MtrunA17_Chr4g0034301 58.389 149 23 1 14 123 9 157 4.73e-51 159
Msa0646650 MtrunA17_Chr1g0170851 37.671 146 55 1 13 122 2 147 1.69e-22 86.7
Msa0646650 MtrunA17_Chr5g0435991 37.241 145 54 2 14 122 3 146 1.88e-22 86.7
Msa0646650 MtrunA17_Chr5g0417631 38.621 145 52 2 14 122 3 146 2.08e-21 84.0
Msa0646650 MtrunA17_Chr5g0435621 35.862 145 56 2 14 122 3 146 2.76e-21 83.6
Msa0646650 MtrunA17_Chr1g0197531 37.241 145 55 1 14 122 3 147 3.28e-21 83.6
Msa0646650 MtrunA17_Chr1g0148751 38.621 145 51 3 14 122 3 145 3.52e-21 83.2
Msa0646650 MtrunA17_Chr5g0435981 38.621 145 51 3 14 122 3 145 4.51e-21 83.2
Msa0646650 MtrunA17_Chr5g0427351 35.616 146 57 2 13 122 2 146 1.85e-20 81.6
Msa0646650 MtrunA17_Chr1g0197501 35.333 150 57 1 13 122 22 171 2.90e-19 79.0
Msa0646650 MtrunA17_Chr5g0435611 35.616 146 57 2 14 123 3 147 1.93e-18 76.3
Msa0646650 MtrunA17_Chr5g0435881 47.887 71 35 2 12 82 1 69 1.48e-12 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0646650 AT2G16060.1 51.634 153 35 1 9 122 4 156 7.20e-47 149
Msa0646650 AT3G10520.1 38.312 154 56 1 10 124 2 155 4.08e-28 101

Find 109 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGAATGGATACAAAATTT+TGG 0.113832 4_3:-52436902 None:intergenic
TACATATGCTACTATATATT+TGG 0.157808 4_3:-52437018 None:intergenic
TTTAAAATTACTTATCAAAT+TGG 0.196171 4_3:-52436210 None:intergenic
ATTATGACATGTTAGAATTC+TGG 0.233407 4_3:+52437516 Msa0646650:intron
GTTAACAAAACAAGCTCTTT+TGG 0.251442 4_3:+52436058 Msa0646650:intron
GGTAACAAAGCAAGCCCTTT+TGG 0.262800 4_3:+52438173 Msa0646650:intron
TCAACTTCAATAAGCTTGTT+TGG 0.263429 4_3:+52438393 Msa0646650:three_prime_UTR
ATTATGATTTGTTAGAATAC+TGG 0.276560 4_3:+52435453 Msa0646650:intron
AGGGCTCTGATGTCATTTAT+TGG 0.282946 4_3:+52436617 Msa0646650:three_prime_UTR
TAAATGATATCTTATGGATT+TGG 0.292211 4_3:-52436717 None:intergenic
AGTTATATGGCAAATGAATT+AGG 0.296700 4_3:-52436253 None:intergenic
TAATAATTGTCCAAGTTATA+TGG 0.311664 4_3:-52436266 None:intergenic
TGTTGCTTCTGACGCTTCTA+TGG 0.316131 4_3:-52436645 None:intergenic
TCTATATAAATGATATCTTA+TGG 0.325424 4_3:-52436723 None:intergenic
ATTAAGTTGCGAACCATTGC+AGG 0.327796 4_3:-52436299 None:intergenic
GAAAGATTCAAATGTTCATT+TGG 0.327999 4_3:+52435513 Msa0646650:CDS
TATATAGTAGCATATGTAAA+AGG 0.328796 4_3:+52437023 Msa0646650:three_prime_UTR
GATTTATATATGTTGCTATT+GGG 0.329197 4_3:+52438447 Msa0646650:three_prime_UTR
AGATGTGGTGTATAATCTTT+TGG 0.332737 4_3:-52436690 None:intergenic
GTCTAATTAAGAGGAGGGTT+TGG 0.334332 4_3:-52438340 None:intergenic
AATGAAAACATTTGCTTAGC+TGG 0.335061 4_3:-52435487 None:intergenic
CAACTTCAATAAGCTTGTTT+GGG 0.339010 4_3:+52438394 Msa0646650:three_prime_UTR
ATAGCTGGAGACCACATTTC+AGG 0.347672 4_3:-52436101 None:intergenic
TGATTTATATATGTTGCTAT+TGG 0.349197 4_3:+52438446 Msa0646650:three_prime_UTR
GCTCTTCAACAAGTTGAATC+AGG 0.353526 4_3:+52436597 Msa0646650:three_prime_UTR
AAAAGATTCAAATGTTCCTT+TGG 0.354441 4_3:+52437576 Msa0646650:three_prime_UTR
CATCTCAGCTTTGATGGCAT+TGG 0.372687 4_3:-52438274 None:intergenic
ATATAATACTCCTAATTTGT+TGG 0.375159 4_3:+52437122 Msa0646650:three_prime_UTR
ATATCTTACACTTGCATTGT+CGG 0.396542 4_3:+52436497 Msa0646650:three_prime_UTR
AATGAGAACAATTGCTTAGC+TGG 0.398732 4_3:-52437550 None:intergenic
GGTGCCACTCATTTCAAAAC+TGG 0.400143 4_3:+52437843 Msa0646650:three_prime_UTR
TTTGGATTTCTCTTCAACAT+TGG 0.401643 4_3:-52438322 None:intergenic
TGAGATGTGGTCACCGGCTA+TGG 0.409008 4_3:+52438218 Msa0646650:three_prime_UTR
AATTCATTTGCCATATAACT+TGG 0.410353 4_3:+52436256 Msa0646650:three_prime_UTR
TTGGTTCAAGTGTGATTAGT+TGG 0.411107 4_3:-52436999 None:intergenic
ATTCACTCATTGATTGAAGA+TGG 0.415873 4_3:-52437202 None:intergenic
TTTATATTATTCAATGATCT+AGG 0.418335 4_3:-52438539 None:intergenic
GAAAGAAGCTCATGATCAAC+TGG 0.424877 4_3:+52436181 Msa0646650:CDS
GACAATGCAAGTGTAAGATA+TGG 0.426053 4_3:-52436495 None:intergenic
TGGCATTGGCCAGCTGATCA+TGG 0.436974 4_3:-52438260 None:intergenic
TATTGTGACCACATAAGAAT+TGG 0.439195 4_3:-52436752 None:intergenic
CTTATGTGGTCACAATACTT+TGG 0.442238 4_3:+52436758 Msa0646650:three_prime_UTR
AATTGAGAGAGAGATTGTTT+AGG 0.452123 4_3:-52436464 None:intergenic
GAATCAAACTTGAAGAAGTT+AGG 0.452597 4_3:+52437822 Msa0646650:three_prime_UTR
ATGGCATGAGGCTTGAGCTT+AGG 0.456443 4_3:-52437607 None:intergenic
CATTTCAGCTTTGATAGCAT+TGG 0.458163 4_3:-52436159 None:intergenic
TGATATCTTATGGATTTGGT+TGG 0.459100 4_3:-52436713 None:intergenic
ATTTATATATGTTGCTATTG+GGG 0.462800 4_3:+52438448 Msa0646650:three_prime_UTR
ACATCGACTATAATAAAAGT+TGG 0.465038 4_3:-52436419 None:intergenic
ATAGCATGAGGCTTAAGCTT+AGG 0.467752 4_3:-52435544 None:intergenic
GGCAGAGGCTCATGATCAGC+TGG 0.480770 4_3:+52436136 Msa0646650:CDS
AGTTGGCCCTTCGTACCAAT+GGG 0.485063 4_3:-52437179 None:intergenic
CTCTGTTATAACACATAACA+TGG 0.486959 4_3:+52435171 Msa0646650:exon
AAGCTCTAGTGGTAAAATCA+TGG 0.489161 4_3:+52435245 Msa0646650:CDS
TGCTATGTCTGTCTTTCTCA+TGG 0.489438 4_3:+52435561 Msa0646650:CDS
AGAAACATAGTCTAATTAAG+AGG 0.496520 4_3:-52438349 None:intergenic
TCTATAAGAAAATATTTGAA+TGG 0.503370 4_3:-52436916 None:intergenic
GATTCAACTTGTTGAAGAGC+GGG 0.503888 4_3:-52436594 None:intergenic
TGCCATGTCTGTCTTTCTCA+TGG 0.508395 4_3:+52437624 Msa0646650:three_prime_UTR
TATGGATTTGGTTGGAGATG+TGG 0.512151 4_3:-52436705 None:intergenic
AAAACATTTGCTTAGCTGGT+GGG 0.513234 4_3:-52435483 None:intergenic
GCAGTTCAATTGAGGAATGC+TGG 0.517566 4_3:+52437783 Msa0646650:three_prime_UTR
GAGTTGGCCCTTCGTACCAA+TGG 0.518325 4_3:-52437180 None:intergenic
TTACAAAGATTGAATAGAGC+AGG 0.519931 4_3:+52436564 Msa0646650:three_prime_UTR
GGGAGAAGCCCATGATCAGC+TGG 0.521253 4_3:+52438251 Msa0646650:three_prime_UTR
AAGCTCTAGTGGTGAAGTCA+TGG 0.522167 4_3:+52437363 Msa0646650:three_prime_UTR
AATTGTGGTGGCAGTGGCAA+TGG 0.527332 4_3:-52435130 None:intergenic
CACCGGCTATGGAAAATGCA+TGG 0.527671 4_3:+52438229 Msa0646650:three_prime_UTR
GGCATTGGCCAGCTGATCAT+GGG 0.529199 4_3:-52438259 None:intergenic
ATAGCCGGTGACCACATCTC+AGG 0.534181 4_3:-52438216 None:intergenic
GTGAATCAGCAGTTCAATTG+AGG 0.534730 4_3:+52437775 Msa0646650:three_prime_UTR
AAACATTTGCTTAGCTGGTG+GGG 0.534952 4_3:-52435482 None:intergenic
ACCGGCTATGGAAAATGCAT+GGG 0.537843 4_3:+52438230 Msa0646650:three_prime_UTR
ATTAAGAATAATAGTGACAA+TGG 0.553831 4_3:-52437289 None:intergenic
CTCTTCAACAAGTTGAATCA+GGG 0.558410 4_3:+52436598 Msa0646650:three_prime_UTR
TTCTTAATCAGCAGTTAACA+TGG 0.559415 4_3:+52437304 Msa0646650:three_prime_UTR
GAGAACAAGAAAACTGTGGA+TGG 0.560503 4_3:+52435196 Msa0646650:CDS
AATGCAATCAAGAACAACTC+TGG 0.563556 4_3:+52437386 Msa0646650:three_prime_UTR
TCATTGATTGAAGATGGAGT+TGG 0.565949 4_3:-52437196 None:intergenic
CTTATTGAAGTTGAGAGCAA+AGG 0.567872 4_3:-52438384 None:intergenic
AGAAGAGCAAGAAGCTCTAG+TGG 0.572167 4_3:+52435234 Msa0646650:CDS
AACATAGTCTAATTAAGAGG+AGG 0.573065 4_3:-52438346 None:intergenic
TTAGTTAGCATGCCCTGCAA+TGG 0.573865 4_3:+52436286 Msa0646650:three_prime_UTR
TTATTAAATTGTGGTGGCAG+TGG 0.574745 4_3:-52435136 None:intergenic
GATCACGACCAATTCTTATG+TGG 0.575601 4_3:+52436744 Msa0646650:three_prime_UTR
GAAAACATTTGCTTAGCTGG+TGG 0.575978 4_3:-52435484 None:intergenic
ACATAGTCTAATTAAGAGGA+GGG 0.576395 4_3:-52438345 None:intergenic
TTGTTTAGGTCAAGTGTGAG+TGG 0.578967 4_3:-52436450 None:intergenic
TTCTTGGCATTGTAGTCAAG+AGG 0.580590 4_3:-52436538 None:intergenic
TAACTCCCCATTGGTACGAA+GGG 0.585772 4_3:+52437173 Msa0646650:three_prime_UTR
ATAACTCCCCATTGGTACGA+AGG 0.587043 4_3:+52437172 Msa0646650:three_prime_UTR
ACTGCAAAATAACTCCCCAT+TGG 0.591136 4_3:+52437164 Msa0646650:three_prime_UTR
AATATAAATAAATTCTACAA+TGG 0.593261 4_3:-52438569 None:intergenic
AATGTTTGTTTATTAAATTG+TGG 0.595500 4_3:-52435145 None:intergenic
ATTACCTGAGATGTGGTCAC+CGG 0.617794 4_3:+52438212 Msa0646650:three_prime_UTR
GAGAACAATTGCTTAGCTGG+TGG 0.618028 4_3:-52437547 None:intergenic
TGATTCAACTTGTTGAAGAG+CGG 0.620586 4_3:-52436595 None:intergenic
AGAGGAACAAGAAGCTCTAG+TGG 0.622190 4_3:+52437352 Msa0646650:three_prime_UTR
TTAAGTTGCGAACCATTGCA+GGG 0.623307 4_3:-52436298 None:intergenic
AAGAAGCATTACCTGAGATG+TGG 0.630182 4_3:+52438205 Msa0646650:three_prime_UTR
AAGAAGCAATACCTGAAATG+TGG 0.642554 4_3:+52436090 Msa0646650:CDS
GTTTGTTTATTAAATTGTGG+TGG 0.658420 4_3:-52435142 None:intergenic
AGAAAGACAGACATGGCATG+AGG 0.665594 4_3:-52437619 None:intergenic
GGAAGAGAACAAGAAAACTG+TGG 0.684809 4_3:+52435192 Msa0646650:CDS
AGAAAGACAGACATAGCATG+AGG 0.685943 4_3:-52435556 None:intergenic
CCGGCTATGGAAAATGCATG+GGG 0.691095 4_3:+52438231 Msa0646650:three_prime_UTR
GTTGGCCCTTCGTACCAATG+GGG 0.706789 4_3:-52437178 None:intergenic
TACAGACAGTTATTTCACAG+AGG 0.726258 4_3:+52437334 Msa0646650:three_prime_UTR
TACCATGAGAAAGACAGACA+TGG 0.729739 4_3:-52437626 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATAATAAATTTTGGTTAT+TGG + chr4_3:52435784-52435803 Msa0646650:intron 10.0%
!!! TTTAAAATTACTTATCAAAT+TGG - chr4_3:52436213-52436232 None:intergenic 10.0%
!!! CTTATATTCTTAATTTTTTT+TGG - chr4_3:52436383-52436402 None:intergenic 10.0%
!!! ATTAATTTCATTTTTCATTT+AGG + chr4_3:52437442-52437461 Msa0646650:intron 10.0%
!! AATATAAATAAATTCTACAA+TGG - chr4_3:52438572-52438591 None:intergenic 10.0%
!! AATGTTTGTTTATTAAATTG+TGG - chr4_3:52435148-52435167 None:intergenic 15.0%
!!! TTAATTAATTAATTTTCAGC+TGG + chr4_3:52435374-52435393 Msa0646650:intron 15.0%
!!! ACCTCTAAAATAATAAATTT+TGG + chr4_3:52435777-52435796 Msa0646650:intron 15.0%
!!! ACCAAAATTTATTATTTTAG+AGG - chr4_3:52435781-52435800 None:intergenic 15.0%
!! TCTATATAAATGATATCTTA+TGG - chr4_3:52436726-52436745 None:intergenic 15.0%
!! TCTATAAGAAAATATTTGAA+TGG - chr4_3:52436919-52436938 None:intergenic 15.0%
!! GTAATTAATCAATAAACAAA+TGG - chr4_3:52437673-52437692 None:intergenic 15.0%
!!! GAGTTTAATCTTTTTAATTA+TGG + chr4_3:52438067-52438086 Msa0646650:intron 15.0%
!! TTTATATTATTCAATGATCT+AGG - chr4_3:52438542-52438561 None:intergenic 15.0%
!!! ATTATGATTTGTTAGAATAC+TGG + chr4_3:52435453-52435472 Msa0646650:intron 20.0%
!! AATTTCTAATTTAAGTCACA+AGG - chr4_3:52435635-52435654 None:intergenic 20.0%
!! TCAAATCATACAATATTGAA+AGG - chr4_3:52435946-52435965 None:intergenic 20.0%
!! TAATAATTGTCCAAGTTATA+TGG - chr4_3:52436269-52436288 None:intergenic 20.0%
!! TAAATGATATCTTATGGATT+TGG - chr4_3:52436720-52436739 None:intergenic 20.0%
!!! TTGAATGGATACAAAATTTT+GGG - chr4_3:52436904-52436923 None:intergenic 20.0%
!!! TTTGAATGGATACAAAATTT+TGG - chr4_3:52436905-52436924 None:intergenic 20.0%
!! TACATATGCTACTATATATT+TGG - chr4_3:52437021-52437040 None:intergenic 20.0%
!! TATATAGTAGCATATGTAAA+AGG + chr4_3:52437023-52437042 Msa0646650:three_prime_UTR 20.0%
!!! TTTTCTTTTTAAGTTTTTGC+AGG - chr4_3:52437062-52437081 None:intergenic 20.0%
!!! ATATAATACTCCTAATTTGT+TGG + chr4_3:52437122-52437141 Msa0646650:three_prime_UTR 20.0%
!! ATTAAGAATAATAGTGACAA+TGG - chr4_3:52437292-52437311 None:intergenic 20.0%
!! TTTACTAGTATATATTGACA+AGG + chr4_3:52437959-52437978 Msa0646650:intron 20.0%
!! AACTCATATTGTGTATATTT+GGG - chr4_3:52438052-52438071 None:intergenic 20.0%
!! AAACTCATATTGTGTATATT+TGG - chr4_3:52438053-52438072 None:intergenic 20.0%
!!! TTTTATTGTTTCGTTATGTA+AGG + chr4_3:52438152-52438171 Msa0646650:intron 20.0%
!! TGATTTATATATGTTGCTAT+TGG + chr4_3:52438446-52438465 Msa0646650:three_prime_UTR 20.0%
!!! GATTTATATATGTTGCTATT+GGG + chr4_3:52438447-52438466 Msa0646650:three_prime_UTR 20.0%
!!! ATTTATATATGTTGCTATTG+GGG + chr4_3:52438448-52438467 Msa0646650:three_prime_UTR 20.0%
!!! ATTGTTGTTTTGCTCTATTA+CGG - chr4_3:52435102-52435121 None:intergenic 25.0%
! GTTTGTTTATTAAATTGTGG+TGG - chr4_3:52435145-52435164 None:intergenic 25.0%
! AATGCAATGAAGAAAAATTC+TGG + chr4_3:52435268-52435287 Msa0646650:CDS 25.0%
! ATGCAATGAAGAAAAATTCT+GGG + chr4_3:52435269-52435288 Msa0646650:CDS 25.0%
! GAAAGATTCAAATGTTCATT+TGG + chr4_3:52435513-52435532 Msa0646650:CDS 25.0%
! CCTCAATTATACTAGTTATA+TGG - chr4_3:52435601-52435620 None:intergenic 25.0%
! CCATATAACTAGTATAATTG+AGG + chr4_3:52435598-52435617 Msa0646650:intron 25.0%
! GAATCAAATTTGAAGAAGTT+AGG + chr4_3:52435862-52435881 Msa0646650:intron 25.0%
!!! TTACATTAGTCTTCTACAAA+AGG - chr4_3:52436006-52436025 None:intergenic 25.0%
! AGTTATATGGCAAATGAATT+AGG - chr4_3:52436256-52436275 None:intergenic 25.0%
! AATTCATTTGCCATATAACT+TGG + chr4_3:52436256-52436275 Msa0646650:three_prime_UTR 25.0%
! AGATGATAATCATGAATCAT+TGG - chr4_3:52436360-52436379 None:intergenic 25.0%
!! ACATCGACTATAATAAAAGT+TGG - chr4_3:52436422-52436441 None:intergenic 25.0%
!! TCAATCTTTGTAAGTTTTCT+TGG - chr4_3:52436557-52436576 None:intergenic 25.0%
! ATTATGACATGTTAGAATTC+TGG + chr4_3:52437516-52437535 Msa0646650:intron 25.0%
! AAAAGATTCAAATGTTCCTT+TGG + chr4_3:52437576-52437595 Msa0646650:three_prime_UTR 25.0%
! GTAATACTAACATACTACTA+TGG + chr4_3:52437888-52437907 Msa0646650:intron 25.0%
!! GTGTATATTTGAAATCATGA+TGG + chr4_3:52437994-52438013 Msa0646650:intron 25.0%
! AGAAACATAGTCTAATTAAG+AGG - chr4_3:52438352-52438371 None:intergenic 25.0%
CTCTGTTATAACACATAACA+TGG + chr4_3:52435171-52435190 Msa0646650:exon 30.0%
TGCAATGAAGAAAAATTCTG+GGG + chr4_3:52435270-52435289 Msa0646650:CDS 30.0%
AATGAAAACATTTGCTTAGC+TGG - chr4_3:52435490-52435509 None:intergenic 30.0%
AGCATGATACTCATAAACTA+CGG + chr4_3:52435675-52435694 Msa0646650:intron 30.0%
TATACTATTTACGACCCTAA+AGG - chr4_3:52435730-52435749 None:intergenic 30.0%
! TGTAAAAGATGAGCATTTTG+AGG + chr4_3:52435906-52435925 Msa0646650:intron 30.0%
! GTTAACAAAACAAGCTCTTT+TGG + chr4_3:52436058-52436077 Msa0646650:intron 30.0%
GTTCGCAACTTAATTGATAA+GGG + chr4_3:52436308-52436327 Msa0646650:intron 30.0%
AATTGAGAGAGAGATTGTTT+AGG - chr4_3:52436467-52436486 None:intergenic 30.0%
ATATCTTACACTTGCATTGT+CGG + chr4_3:52436497-52436516 Msa0646650:three_prime_UTR 30.0%
TTACAAAGATTGAATAGAGC+AGG + chr4_3:52436564-52436583 Msa0646650:three_prime_UTR 30.0%
!! TTTTGGTGGTTATGAATCAT+TGG - chr4_3:52436676-52436695 None:intergenic 30.0%
! AGATGTGGTGTATAATCTTT+TGG - chr4_3:52436693-52436712 None:intergenic 30.0%
! TGATATCTTATGGATTTGGT+TGG - chr4_3:52436716-52436735 None:intergenic 30.0%
TATTGTGACCACATAAGAAT+TGG - chr4_3:52436755-52436774 None:intergenic 30.0%
! AAAATTTTGGGCACAAGATT+TGG - chr4_3:52436892-52436911 None:intergenic 30.0%
!!! TGTGCTTTTTCCAACAAATT+AGG - chr4_3:52437135-52437154 None:intergenic 30.0%
! ATTCACTCATTGATTGAAGA+TGG - chr4_3:52437205-52437224 None:intergenic 30.0%
TTCTTAATCAGCAGTTAACA+TGG + chr4_3:52437304-52437323 Msa0646650:three_prime_UTR 30.0%
GAATCAAACTTGAAGAAGTT+AGG + chr4_3:52437822-52437841 Msa0646650:three_prime_UTR 30.0%
! TGTAAAAGATGAGCATTTTG+AGG + chr4_3:52435906-52435925 Msa0646650:intron 30.0%
! GTTCTTCTAGAAAAGTAGTA+CGG - chr4_3:52438108-52438127 None:intergenic 30.0%
! TTCTTTTATGGTCTCCAAAA+GGG - chr4_3:52438190-52438209 None:intergenic 30.0%
!!! TTTTTTCATCTCAGCTTTGA+TGG - chr4_3:52438283-52438302 None:intergenic 30.0%
TTTGGATTTCTCTTCAACAT+TGG - chr4_3:52438325-52438344 None:intergenic 30.0%
ACATAGTCTAATTAAGAGGA+GGG - chr4_3:52438348-52438367 None:intergenic 30.0%
AACATAGTCTAATTAAGAGG+AGG - chr4_3:52438349-52438368 None:intergenic 30.0%
! TCAACTTCAATAAGCTTGTT+TGG + chr4_3:52438393-52438412 Msa0646650:three_prime_UTR 30.0%
! CAACTTCAATAAGCTTGTTT+GGG + chr4_3:52438394-52438413 Msa0646650:three_prime_UTR 30.0%
!! TGGGGTTTGATAAGTTTTAA+AGG + chr4_3:52438466-52438485 Msa0646650:three_prime_UTR 30.0%
TTATTAAATTGTGGTGGCAG+TGG - chr4_3:52435139-52435158 None:intergenic 35.0%
! AAGCTCTAGTGGTAAAATCA+TGG + chr4_3:52435245-52435264 Msa0646650:CDS 35.0%
AAAACATTTGCTTAGCTGGT+GGG - chr4_3:52435486-52435505 None:intergenic 35.0%
ATGAGTATCATGCTAGTAAC+TGG - chr4_3:52435669-52435688 None:intergenic 35.0%
! TCATGCTAACTTGTACCTTT+AGG + chr4_3:52435712-52435731 Msa0646650:intron 35.0%
! CATGCTAACTTGTACCTTTA+GGG + chr4_3:52435713-52435732 Msa0646650:intron 35.0%
AAGAAGCAATACCTGAAATG+TGG + chr4_3:52436090-52436109 Msa0646650:CDS 35.0%
TCCAGCTATGAAAAATGCAT+GGG + chr4_3:52436115-52436134 Msa0646650:CDS 35.0%
! CATTTCAGCTTTGATAGCAT+TGG - chr4_3:52436162-52436181 None:intergenic 35.0%
GGTTCGCAACTTAATTGATA+AGG + chr4_3:52436307-52436326 Msa0646650:intron 35.0%
GACAATGCAAGTGTAAGATA+TGG - chr4_3:52436498-52436517 None:intergenic 35.0%
TGCATTGTCGGTAAAAACAA+CGG + chr4_3:52436509-52436528 Msa0646650:three_prime_UTR 35.0%
!! TGATTCAACTTGTTGAAGAG+CGG - chr4_3:52436598-52436617 None:intergenic 35.0%
! CTCTTCAACAAGTTGAATCA+GGG + chr4_3:52436598-52436617 Msa0646650:three_prime_UTR 35.0%
! TGTGGTGTATAATCTTTTGG+TGG - chr4_3:52436690-52436709 None:intergenic 35.0%
CTTATGTGGTCACAATACTT+TGG + chr4_3:52436758-52436777 Msa0646650:three_prime_UTR 35.0%
TTGGTTCAAGTGTGATTAGT+TGG - chr4_3:52437002-52437021 None:intergenic 35.0%
! TCATTGATTGAAGATGGAGT+TGG - chr4_3:52437199-52437218 None:intergenic 35.0%
! TACAGACAGTTATTTCACAG+AGG + chr4_3:52437334-52437353 Msa0646650:three_prime_UTR 35.0%
AATGCAATCAAGAACAACTC+TGG + chr4_3:52437386-52437405 Msa0646650:three_prime_UTR 35.0%
AATGAGAACAATTGCTTAGC+TGG - chr4_3:52437553-52437572 None:intergenic 35.0%
!!! TACACCAGTTTTGAAATGAG+TGG - chr4_3:52437850-52437869 None:intergenic 35.0%
ACTATGGTTGACACAATGTT+AGG + chr4_3:52437904-52437923 Msa0646650:intron 35.0%
AAAAGACTTGTACATTCGCA+CGG - chr4_3:52437942-52437961 None:intergenic 35.0%
! ATTGTGTATATTTGGGTTCG+TGG - chr4_3:52438045-52438064 None:intergenic 35.0%
! CTTCTTTTATGGTCTCCAAA+AGG - chr4_3:52438191-52438210 None:intergenic 35.0%
! CTCAGGTAATGCTTCTTTTA+TGG - chr4_3:52438202-52438221 None:intergenic 35.0%
CTTATTGAAGTTGAGAGCAA+AGG - chr4_3:52438387-52438406 None:intergenic 35.0%
GGAAGAGAACAAGAAAACTG+TGG + chr4_3:52435192-52435211 Msa0646650:CDS 40.0%
GAGAACAAGAAAACTGTGGA+TGG + chr4_3:52435196-52435215 Msa0646650:CDS 40.0%
TCGTGAAAAACTTGAGACTC+AGG - chr4_3:52435297-52435316 None:intergenic 40.0%
CATGAGTGATACTACTCAGT+TGG - chr4_3:52435424-52435443 None:intergenic 40.0%
AAACATTTGCTTAGCTGGTG+GGG - chr4_3:52435485-52435504 None:intergenic 40.0%
GAAAACATTTGCTTAGCTGG+TGG - chr4_3:52435487-52435506 None:intergenic 40.0%
ATAGCATGAGGCTTAAGCTT+AGG - chr4_3:52435547-52435566 None:intergenic 40.0%
AGAAAGACAGACATAGCATG+AGG - chr4_3:52435559-52435578 None:intergenic 40.0%
TGCTATGTCTGTCTTTCTCA+TGG + chr4_3:52435561-52435580 Msa0646650:CDS 40.0%
GGTGACACTCATTTCAAATC+TGG + chr4_3:52435883-52435902 Msa0646650:intron 40.0%
!! ACAAGCTCTTTTGGAGACAA+TGG + chr4_3:52436067-52436086 Msa0646650:CDS 40.0%
CTCCAGCTATGAAAAATGCA+TGG + chr4_3:52436114-52436133 Msa0646650:CDS 40.0%
TATGAAAAATGCATGGGCAG+AGG + chr4_3:52436121-52436140 Msa0646650:CDS 40.0%
GAAAGAAGCTCATGATCAAC+TGG + chr4_3:52436181-52436200 Msa0646650:CDS 40.0%
! TTAAGTTGCGAACCATTGCA+GGG - chr4_3:52436301-52436320 None:intergenic 40.0%
! ATTAAGTTGCGAACCATTGC+AGG - chr4_3:52436302-52436321 None:intergenic 40.0%
TTGTTTAGGTCAAGTGTGAG+TGG - chr4_3:52436453-52436472 None:intergenic 40.0%
TTCTTGGCATTGTAGTCAAG+AGG - chr4_3:52436541-52436560 None:intergenic 40.0%
!! GATTCAACTTGTTGAAGAGC+GGG - chr4_3:52436597-52436616 None:intergenic 40.0%
! GCTCTTCAACAAGTTGAATC+AGG + chr4_3:52436597-52436616 Msa0646650:three_prime_UTR 40.0%
! AGGGCTCTGATGTCATTTAT+TGG + chr4_3:52436617-52436636 Msa0646650:three_prime_UTR 40.0%
! TATGGATTTGGTTGGAGATG+TGG - chr4_3:52436708-52436727 None:intergenic 40.0%
GATCACGACCAATTCTTATG+TGG + chr4_3:52436744-52436763 Msa0646650:three_prime_UTR 40.0%
ACTGCAAAATAACTCCCCAT+TGG + chr4_3:52437164-52437183 Msa0646650:three_prime_UTR 40.0%
! GTGCTGTGGTACAGTTTTTA+TGG - chr4_3:52437239-52437258 None:intergenic 40.0%
!! TGATTTTGTTGCTTGTGCTG+TGG - chr4_3:52437253-52437272 None:intergenic 40.0%
! AGCTTAGGATTTTGCTCCAA+AGG - chr4_3:52435532-52435551 None:intergenic 40.0%
TACCATGAGAAAGACAGACA+TGG - chr4_3:52435566-52435585 None:intergenic 40.0%
TGGATGCTAAGTGTTAAGCA+AGG - chr4_3:52437653-52437672 None:intergenic 40.0%
GTGAATCAGCAGTTCAATTG+AGG + chr4_3:52437775-52437794 Msa0646650:three_prime_UTR 40.0%
!! AAGAAGCATTACCTGAGATG+TGG + chr4_3:52438205-52438224 Msa0646650:three_prime_UTR 40.0%
GTCTAATTAAGAGGAGGGTT+TGG - chr4_3:52438343-52438362 None:intergenic 40.0%
! AGAAGAGCAAGAAGCTCTAG+TGG + chr4_3:52435234-52435253 Msa0646650:CDS 45.0%
ATAGCTGGAGACCACATTTC+AGG - chr4_3:52436104-52436123 None:intergenic 45.0%
! GCCCATGCATTTTTCATAGC+TGG - chr4_3:52436119-52436138 None:intergenic 45.0%
TTAGTTAGCATGCCCTGCAA+TGG + chr4_3:52436286-52436305 Msa0646650:three_prime_UTR 45.0%
! TTGATAAGGGAGAGCATGTG+TGG + chr4_3:52436321-52436340 Msa0646650:intron 45.0%
! TGATAAGGGAGAGCATGTGT+GGG + chr4_3:52436322-52436341 Msa0646650:intron 45.0%
! TGTTGCTTCTGACGCTTCTA+TGG - chr4_3:52436648-52436667 None:intergenic 45.0%
ATAACTCCCCATTGGTACGA+AGG + chr4_3:52437172-52437191 Msa0646650:three_prime_UTR 45.0%
TAACTCCCCATTGGTACGAA+GGG + chr4_3:52437173-52437192 Msa0646650:three_prime_UTR 45.0%
! AGAGGAACAAGAAGCTCTAG+TGG + chr4_3:52437352-52437371 Msa0646650:three_prime_UTR 45.0%
! AAGCTCTAGTGGTGAAGTCA+TGG + chr4_3:52437363-52437382 Msa0646650:three_prime_UTR 45.0%
GAGAACAATTGCTTAGCTGG+TGG - chr4_3:52437550-52437569 None:intergenic 45.0%
AGAAAGACAGACATGGCATG+AGG - chr4_3:52437622-52437641 None:intergenic 45.0%
TGCCATGTCTGTCTTTCTCA+TGG + chr4_3:52437624-52437643 Msa0646650:three_prime_UTR 45.0%
GCAGTTCAATTGAGGAATGC+TGG + chr4_3:52437783-52437802 Msa0646650:three_prime_UTR 45.0%
!! GGTGCCACTCATTTCAAAAC+TGG + chr4_3:52437843-52437862 Msa0646650:three_prime_UTR 45.0%
! GGTAACAAAGCAAGCCCTTT+TGG + chr4_3:52438173-52438192 Msa0646650:intron 45.0%
ATTACCTGAGATGTGGTCAC+CGG + chr4_3:52438212-52438231 Msa0646650:three_prime_UTR 45.0%
ACCGGCTATGGAAAATGCAT+GGG + chr4_3:52438230-52438249 Msa0646650:three_prime_UTR 45.0%
! CATCTCAGCTTTGATGGCAT+TGG - chr4_3:52438277-52438296 None:intergenic 45.0%
!!! ATTTATATTCATTTTATTAT+AGG + chr4_3:52438583-52438602 Msa0646650:three_prime_UTR 5.0%
AATTGTGGTGGCAGTGGCAA+TGG - chr4_3:52435133-52435152 None:intergenic 50.0%
AGTTGGCCCTTCGTACCAAT+GGG - chr4_3:52437182-52437201 None:intergenic 50.0%
ATGGCATGAGGCTTGAGCTT+AGG - chr4_3:52437610-52437629 None:intergenic 50.0%
TGTACATTCGCACGGATGCA+CGG - chr4_3:52437934-52437953 None:intergenic 50.0%
! CACCGGCTATGGAAAATGCA+TGG + chr4_3:52438229-52438248 Msa0646650:three_prime_UTR 50.0%
! CCCCATGCATTTTCCATAGC+CGG - chr4_3:52438234-52438253 None:intergenic 50.0%
CCGGCTATGGAAAATGCATG+GGG + chr4_3:52438231-52438250 Msa0646650:three_prime_UTR 50.0%
GTCAGCAGTTCAACTGCGCA+AGG + chr4_3:52435819-52435838 Msa0646650:intron 55.0%
GTTGGCCCTTCGTACCAATG+GGG - chr4_3:52437181-52437200 None:intergenic 55.0%
GAGTTGGCCCTTCGTACCAA+TGG - chr4_3:52437183-52437202 None:intergenic 55.0%
ATAGCCGGTGACCACATCTC+AGG - chr4_3:52438219-52438238 None:intergenic 55.0%
! TGAGATGTGGTCACCGGCTA+TGG + chr4_3:52438218-52438237 Msa0646650:three_prime_UTR 55.0%
!! GGCATTGGCCAGCTGATCAT+GGG - chr4_3:52438262-52438281 None:intergenic 55.0%
!! TGGCATTGGCCAGCTGATCA+TGG - chr4_3:52438263-52438282 None:intergenic 55.0%
GCAGTTCAACTGCGCAAGGC+TGG + chr4_3:52435823-52435842 Msa0646650:intron 60.0%
GGCAGAGGCTCATGATCAGC+TGG + chr4_3:52436136-52436155 Msa0646650:CDS 60.0%
GGGAGAAGCCCATGATCAGC+TGG + chr4_3:52438251-52438270 Msa0646650:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 52435081 52438619 52435081 ID=Msa0646650;Name=Msa0646650
chr4_3 mRNA 52435081 52438619 52435081 ID=Msa0646650-mRNA-1;Parent=Msa0646650;Name=Msa0646650-mRNA-1;_AED=0.09;_eAED=0.09;_QI=109|0.83|1|1|0.83|0.71|7|1815|140
chr4_3 exon 52435081 52435317 52435081 ID=Msa0646650-mRNA-1:exon:12855;Parent=Msa0646650-mRNA-1
chr4_3 exon 52435468 52435582 52435468 ID=Msa0646650-mRNA-1:exon:12856;Parent=Msa0646650-mRNA-1
chr4_3 exon 52436059 52436323 52436059 ID=Msa0646650-mRNA-1:exon:12857;Parent=Msa0646650-mRNA-1
chr4_3 exon 52436384 52437435 52436384 ID=Msa0646650-mRNA-1:exon:12858;Parent=Msa0646650-mRNA-1
chr4_3 exon 52437531 52437645 52437531 ID=Msa0646650-mRNA-1:exon:12859;Parent=Msa0646650-mRNA-1
chr4_3 exon 52437771 52437887 52437771 ID=Msa0646650-mRNA-1:exon:12860;Parent=Msa0646650-mRNA-1
chr4_3 exon 52438174 52438619 52438174 ID=Msa0646650-mRNA-1:exon:12861;Parent=Msa0646650-mRNA-1
chr4_3 five_prime_UTR 52435081 52435189 52435081 ID=Msa0646650-mRNA-1:five_prime_utr;Parent=Msa0646650-mRNA-1
chr4_3 CDS 52435190 52435317 52435190 ID=Msa0646650-mRNA-1:cds;Parent=Msa0646650-mRNA-1
chr4_3 CDS 52435468 52435582 52435468 ID=Msa0646650-mRNA-1:cds;Parent=Msa0646650-mRNA-1
chr4_3 CDS 52436059 52436238 52436059 ID=Msa0646650-mRNA-1:cds;Parent=Msa0646650-mRNA-1
chr4_3 three_prime_UTR 52436239 52436323 52436239 ID=Msa0646650-mRNA-1:three_prime_utr;Parent=Msa0646650-mRNA-1
chr4_3 three_prime_UTR 52436384 52437435 52436384 ID=Msa0646650-mRNA-1:three_prime_utr;Parent=Msa0646650-mRNA-1
chr4_3 three_prime_UTR 52437531 52437645 52437531 ID=Msa0646650-mRNA-1:three_prime_utr;Parent=Msa0646650-mRNA-1
chr4_3 three_prime_UTR 52437771 52437887 52437771 ID=Msa0646650-mRNA-1:three_prime_utr;Parent=Msa0646650-mRNA-1
chr4_3 three_prime_UTR 52438174 52438619 52438174 ID=Msa0646650-mRNA-1:three_prime_utr;Parent=Msa0646650-mRNA-1
Gene Sequence

>Msa0646650

ATGGAAGAGAACAAGAAAACTGTGGATGGAAGTGTTTGTTTCACAGAAGAGCAAGAAGCTCTAGTGGTAAAATCATGGAATGCAATGAAGAAAAATTCTGGGGACCTGAGTCTCAAGTTTTTCACGAAAATACTGGAGATTGCCCCACCAGCTAAGCAAATGTTTTCATTCTTGAAAGATTCAAATGTTCATTTGGAGCAAAATCCTAAGCTTAAGCCTCATGCTATGTCTGTCTTTCTCATGTTAACAAAACAAGCTCTTTTGGAGACAATGGAAGAAGCAATACCTGAAATGTGGTCTCCAGCTATGAAAAATGCATGGGCAGAGGCTCATGATCAGCTGGCCAATGCTATCAAAGCTGAAATGAAAGAAGCTCATGATCAACTGGACAATGCCAATTTGATAAGTAATTTTAAATTTTAA
Protein sequence

>Msa0646650

MEENKKTVDGSVCFTEEQEALVVKSWNAMKKNSGDLSLKFFTKILEIAPPAKQMFSFLKDSNVHLEQNPKLKPHAMSVFLMLTKQALLETMEEAIPEMWSPAMKNAWAEAHDQLANAIKAEMKEAHDQLDNANLISNFKF*