AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0112550

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0112550 MtrunA17_Chr1g0170671 90.566 212 17 1 1 209 1 212 2.33e-143 398
Msa0112550 MtrunA17_Chr5g0403911 39.375 160 86 3 56 205 26 184 2.73e-30 110
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0112550 AT5G51110.2 68.605 172 53 1 39 209 22 193 6.06e-87 255
Msa0112550 AT5G51110.1 64.021 189 66 2 23 209 32 220 1.98e-85 252
Msa0112550 AT1G29810.2 38.211 123 75 1 85 206 23 145 2.20e-28 104
Msa0112550 AT1G29810.1 38.655 119 72 1 89 206 66 184 5.38e-28 104

Find 51 sgRNAs with CRISPR-Local

Find 181 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGAAAGAGCAGAGATATT+TGG 0.213526 1_3:+27538169 None:intergenic
TGCTCTTTCTCTTTCTCAAC+AGG 0.290739 1_3:-27538158 Msa0112550:CDS
GAGAAAGAGCAGAGATATTT+GGG 0.293549 1_3:+27538170 None:intergenic
GGTTGTTCTATGTGGATATT+GGG 0.302096 1_3:+27536188 None:intergenic
AGTTCTGCTCTAACTTGATT+TGG 0.315123 1_3:+27536167 None:intergenic
TGGTTGTTCTATGTGGATAT+TGG 0.345309 1_3:+27536187 None:intergenic
AGGGTTGAGTTTGGTGGTGT+TGG 0.350306 1_3:+27538360 None:intergenic
GGGTTGAGTTTGGTGGTGTT+GGG 0.361314 1_3:+27538361 None:intergenic
GAGCAGAGATATTTGGGATA+AGG 0.364640 1_3:+27538176 None:intergenic
CAATTCGGAGAGAAAGTTAT+AGG 0.381204 1_3:-27538223 Msa0112550:CDS
GGGGACTGTAAAGGTGAAAT+GGG 0.394511 1_3:+27538127 None:intergenic
ATTGAATGAAGGGTTGAGTT+TGG 0.402466 1_3:+27538351 None:intergenic
ATCAAGTTAGAGCAGAACTA+TGG 0.405443 1_3:-27536163 Msa0112550:CDS
AATTGACTTTCCAACTCAGC+TGG 0.437285 1_3:+27538241 None:intergenic
CTTGATTTGGTTGTTCTATG+TGG 0.450240 1_3:+27536180 None:intergenic
ATGTTACCTTTCTTATAAGC+TGG 0.450280 1_3:+27538083 None:intergenic
TTTCAGAACCGTACTCCAAA+CGG 0.461066 1_3:-27538328 Msa0112550:CDS
TTCAGAACCGTACTCCAAAC+GGG 0.462057 1_3:-27538327 Msa0112550:CDS
CGAAGTGGAGAGAACCCGTT+TGG 0.465122 1_3:+27538313 None:intergenic
TGTCCCAAGGAAAAGAGCTT+GGG 0.472741 1_3:-27535470 Msa0112550:CDS
CTTTCCAACTCAGCTGGGAA+AGG 0.477753 1_3:+27538247 None:intergenic
GGGACTGTAAAGGTGAAATG+GGG 0.478411 1_3:+27538128 None:intergenic
CGGTTCTGAAAATTGAATGA+AGG 0.495691 1_3:+27538340 None:intergenic
TTGGGATTTATCTGTAACAC+GGG 0.496700 1_3:+27538379 None:intergenic
GAGAGAACCCGTTTGGAGTA+CGG 0.497420 1_3:+27538320 None:intergenic
TACTTAAACTTCGATGCTTG+TGG 0.499142 1_3:-27536286 Msa0112550:CDS
TGCTAACCAGCTTATAAGAA+AGG 0.502324 1_3:-27538089 Msa0112550:intron
GGTTAGCATCATCTTGAGAA+AGG 0.509678 1_3:+27538104 None:intergenic
GCTGAGTTGGAAAGTCAATT+CGG 0.516869 1_3:-27538238 Msa0112550:CDS
AGACCCTTTCCCAGCTGAGT+TGG 0.518212 1_3:-27538251 Msa0112550:CDS
TTTCCAACTCAGCTGGGAAA+GGG 0.519124 1_3:+27538248 None:intergenic
GTTAGCATCATCTTGAGAAA+GGG 0.522512 1_3:+27538105 None:intergenic
TTGTCCCAAGGAAAAGAGCT+TGG 0.523587 1_3:-27535471 Msa0112550:CDS
GGTTCTGAAAATTGAATGAA+GGG 0.540341 1_3:+27538341 None:intergenic
GAAGGATAATAATACAAGCT+TGG 0.542503 1_3:+27538402 None:intergenic
ATTGACTTTCCAACTCAGCT+GGG 0.560497 1_3:+27538242 None:intergenic
ATTTATCTGTAACACGGGGA+AGG 0.574185 1_3:+27538384 None:intergenic
TGGAGACTTGTGAATGATGA+AGG 0.574412 1_3:-27536311 Msa0112550:CDS
GAATGAAGGGTTGAGTTTGG+TGG 0.578352 1_3:+27538354 None:intergenic
TCTACAGTTGTAGAGACTGC+AGG 0.582828 1_3:-27536218 Msa0112550:CDS
GTTGTTCTATGTGGATATTG+GGG 0.611420 1_3:+27536189 None:intergenic
GGACTGTAAAGGTGAAATGG+GGG 0.615160 1_3:+27538129 None:intergenic
AGCATCATCTTGAGAAAGGG+GGG 0.631020 1_3:+27538108 None:intergenic
TTAGCATCATCTTGAGAAAG+GGG 0.631573 1_3:+27538106 None:intergenic
CAAACGGGTTCTCTCCACTT+CGG 0.636865 1_3:-27538312 Msa0112550:CDS
TAGCATCATCTTGAGAAAGG+GGG 0.654155 1_3:+27538107 None:intergenic
GAACTATGGACAACATCCAT+CGG 0.659072 1_3:-27536149 Msa0112550:intron
GTTGGGATTTATCTGTAACA+CGG 0.665689 1_3:+27538378 None:intergenic
AGACATCAGATCTTGTCCCA+AGG 0.671698 1_3:-27535483 Msa0112550:CDS
TCGTTGTTAAAAGACCGAAG+TGG 0.708298 1_3:+27538298 None:intergenic
TGGGATTTATCTGTAACACG+GGG 0.751053 1_3:+27538380 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTTGTAATAATTTGAAAAA+TGG - chr1_3:27535874-27535893 Msa0112550:intron 15.0%
!!! TCATAATCAAATTAACATTT+TGG + chr1_3:27536616-27536635 None:intergenic 15.0%
!! ATAAAACATCTAAATAGTTT+AGG + chr1_3:27537315-27537334 None:intergenic 15.0%
!!! CATATAAAAAATGTCTTTTA+AGG + chr1_3:27537953-27537972 None:intergenic 15.0%
!! TTTATAACTGTCTAAAAAAG+TGG + chr1_3:27535962-27535981 None:intergenic 20.0%
!! ATGAACATGTGTTTATAAAT+GGG + chr1_3:27536129-27536148 None:intergenic 20.0%
!! AATGAACATGTGTTTATAAA+TGG + chr1_3:27536130-27536149 None:intergenic 20.0%
!! ATATATAAGCTATAAAGACT+AGG - chr1_3:27536269-27536288 Msa0112550:CDS 20.0%
!! TTTAAATGATGCATAAAATC+TGG - chr1_3:27536793-27536812 Msa0112550:intron 20.0%
!!! TATTTTCCAAATGAAAATAC+AGG + chr1_3:27537046-27537065 None:intergenic 20.0%
!!! TTCATTTTTTGTAGAGTTTT+TGG - chr1_3:27537408-27537427 Msa0112550:intron 20.0%
!!! AAATTGAGAGATTTTAAATG+CGG - chr1_3:27537614-27537633 Msa0112550:intron 20.0%
!! AAAATCATATAAGCACATAT+AGG - chr1_3:27538247-27538266 Msa0112550:CDS 20.0%
!! TCTTTTAACAACGATTTCTT+GGG - chr1_3:27535588-27535607 Msa0112550:intron 25.0%
! TGAACATGTGTTTATAAATG+GGG + chr1_3:27536128-27536147 None:intergenic 25.0%
!! ACCACCAAACTAATATTTTT+GGG + chr1_3:27536296-27536315 None:intergenic 25.0%
!! TACCACCAAACTAATATTTT+TGG + chr1_3:27536297-27536316 None:intergenic 25.0%
!! CTTTTATAGACAAATGTTTG+TGG + chr1_3:27536409-27536428 None:intergenic 25.0%
! AGAAAAACGTTATAAGACAT+CGG - chr1_3:27536427-27536446 Msa0112550:intron 25.0%
! TTGGTAAAAGTTAAGATGAA+AGG + chr1_3:27536535-27536554 None:intergenic 25.0%
!! ATCTTAACTTTTACCAAACA+AGG - chr1_3:27536538-27536557 Msa0112550:intron 25.0%
!!! TCAAATTAACATTTTGGTCA+AGG + chr1_3:27536610-27536629 None:intergenic 25.0%
! TGTACATATACTCTATTGAA+TGG + chr1_3:27536701-27536720 None:intergenic 25.0%
!! TAGTTAAGTGTGTCAATTTT+TGG - chr1_3:27536914-27536933 Msa0112550:intron 25.0%
! TTTAAGAAGCAAGTGTAAAA+TGG + chr1_3:27537514-27537533 None:intergenic 25.0%
!! GTTTTTCTTTTCATTTATGC+AGG - chr1_3:27537535-27537554 Msa0112550:intron 25.0%
!! TTTTCATTTATGCAGGTTTT+GGG - chr1_3:27537542-27537561 Msa0112550:intron 25.0%
! AAGGAAAAAGGAATGATAAA+GGG + chr1_3:27538216-27538235 None:intergenic 25.0%
!!! CAAGATGAGAACTTATTTTT+AGG + chr1_3:27538281-27538300 None:intergenic 25.0%
GAAGGATAATAATACAAGCT+TGG + chr1_3:27535478-27535497 None:intergenic 30.0%
!! GGTTCTGAAAATTGAATGAA+GGG + chr1_3:27535539-27535558 None:intergenic 30.0%
! GTCTTTTAACAACGATTTCT+TGG - chr1_3:27535587-27535606 Msa0112550:intron 30.0%
! CTTTTAACAACGATTTCTTG+GGG - chr1_3:27535589-27535608 Msa0112550:intron 30.0%
! TTTTAACAACGATTTCTTGG+GGG - chr1_3:27535590-27535609 Msa0112550:intron 30.0%
ATGTTACCTTTCTTATAAGC+TGG + chr1_3:27535797-27535816 None:intergenic 30.0%
TCTCTAAGCAACTATTTACA+CGG - chr1_3:27535823-27535842 Msa0112550:intron 30.0%
CGTGTTAGTGTTAGAATATT+TGG - chr1_3:27535918-27535937 Msa0112550:intron 30.0%
!!! ATCTGTTTTAGAAATCGTGT+CGG - chr1_3:27536058-27536077 Msa0112550:intron 30.0%
GAACATGTGTTTATAAATGG+GGG + chr1_3:27536127-27536146 None:intergenic 30.0%
AGTGTTAAAGAATCTCACAT+CGG + chr1_3:27536156-27536175 None:intergenic 30.0%
AACTATATTACCTTCCAACA+TGG + chr1_3:27536194-27536213 None:intergenic 30.0%
AGGTCCCAAAAATATTAGTT+TGG - chr1_3:27536289-27536308 Msa0112550:CDS 30.0%
! TCCCAAAAATATTAGTTTGG+TGG - chr1_3:27536292-27536311 Msa0112550:CDS 30.0%
!! GTTTGTGGTTAATTTGTACA+AGG + chr1_3:27536394-27536413 None:intergenic 30.0%
CTCACAGATTACTTACAAAT+GGG + chr1_3:27536475-27536494 None:intergenic 30.0%
ACTCACAGATTACTTACAAA+TGG + chr1_3:27536476-27536495 None:intergenic 30.0%
! AAGCTTACATTTTCCTTGTT+TGG + chr1_3:27536554-27536573 None:intergenic 30.0%
ATATGAACTATGAACCACTA+AGG - chr1_3:27536747-27536766 Msa0112550:intron 30.0%
TATGAACTATGAACCACTAA+GGG - chr1_3:27536748-27536767 Msa0112550:intron 30.0%
! GCACATTTGATTATCAGTTT+TGG - chr1_3:27536945-27536964 Msa0112550:intron 30.0%
ATTGCAAAGATATTGCTTTC+AGG - chr1_3:27537003-27537022 Msa0112550:intron 30.0%
! GTTGAACCTGTATTTTCATT+TGG - chr1_3:27537037-27537056 Msa0112550:intron 30.0%
AACATGATATACAAGTGTTC+AGG - chr1_3:27537065-27537084 Msa0112550:intron 30.0%
! CTTTTCATTTATGCAGGTTT+TGG - chr1_3:27537541-27537560 Msa0112550:intron 30.0%
AAAACTGAGCTAAGAATGAT+AGG + chr1_3:27537790-27537809 None:intergenic 30.0%
TTTCAGTATTACATTAACCG+TGG - chr1_3:27537845-27537864 Msa0112550:intron 30.0%
!!! GGTAATACAAGCGTTTTTTT+AGG + chr1_3:27537979-27537998 None:intergenic 30.0%
TTGTATTACCAACATTACGT+TGG - chr1_3:27537989-27538008 Msa0112550:intron 30.0%
! TATTTCTGTAACGACGTTAT+TGG + chr1_3:27538164-27538183 None:intergenic 30.0%
! AGAGACATTTTGTAACTCAA+AGG - chr1_3:27538184-27538203 Msa0112550:CDS 30.0%
! GAGACATTTTGTAACTCAAA+GGG - chr1_3:27538185-27538204 Msa0112550:CDS 30.0%
GAAGGAAAAAGGAATGATAA+AGG + chr1_3:27538217-27538236 None:intergenic 30.0%
! ATGATTTTCGTTAGTGAAGA+AGG + chr1_3:27538235-27538254 None:intergenic 30.0%
! TTGAAAGCATGACATTTGAA+AGG - chr1_3:27538304-27538323 Msa0112550:CDS 30.0%
! GAAAGGCTTCAATATTTTTC+AGG - chr1_3:27538321-27538340 Msa0112550:CDS 30.0%
TTGGGATTTATCTGTAACAC+GGG + chr1_3:27535501-27535520 None:intergenic 35.0%
GTTGGGATTTATCTGTAACA+CGG + chr1_3:27535502-27535521 None:intergenic 35.0%
ATTGAATGAAGGGTTGAGTT+TGG + chr1_3:27535529-27535548 None:intergenic 35.0%
!! CGGTTCTGAAAATTGAATGA+AGG + chr1_3:27535540-27535559 None:intergenic 35.0%
CAATTCGGAGAGAAAGTTAT+AGG - chr1_3:27535654-27535673 Msa0112550:intron 35.0%
! TGGGATAAGGATTTTGTGTT+CGG + chr1_3:27535691-27535710 None:intergenic 35.0%
GAGAAAGAGCAGAGATATTT+GGG + chr1_3:27535710-27535729 None:intergenic 35.0%
AGAGAAAGAGCAGAGATATT+TGG + chr1_3:27535711-27535730 None:intergenic 35.0%
TTAGCATCATCTTGAGAAAG+GGG + chr1_3:27535774-27535793 None:intergenic 35.0%
GTTAGCATCATCTTGAGAAA+GGG + chr1_3:27535775-27535794 None:intergenic 35.0%
TGCTAACCAGCTTATAAGAA+AGG - chr1_3:27535788-27535807 Msa0112550:intron 35.0%
TATTCTAACACTAACACGTG+TGG + chr1_3:27535916-27535935 None:intergenic 35.0%
GAGATGAAGATGTTTGAATC+AGG - chr1_3:27536025-27536044 Msa0112550:intron 35.0%
!!! TCTGTTTTAGAAATCGTGTC+GGG - chr1_3:27536059-27536078 Msa0112550:intron 35.0%
TGAGATTACATTCTCCATGT+TGG - chr1_3:27536177-27536196 Msa0112550:CDS 35.0%
ATTACATTCTCCATGTTGGA+AGG - chr1_3:27536181-27536200 Msa0112550:CDS 35.0%
! CAAGTAAGGATTAAAGCACT+TGG - chr1_3:27536243-27536262 Msa0112550:CDS 35.0%
! ATTAGTTTGGTGGTATGAGT+TGG - chr1_3:27536302-27536321 Msa0112550:CDS 35.0%
!! AACACTGAAGAAGTGCTATT+AGG - chr1_3:27536325-27536344 Msa0112550:intron 35.0%
AGGATCATTAACTCAAGTTC+AGG + chr1_3:27536590-27536609 None:intergenic 35.0%
ATGGCATAGCATATACATCA+TGG + chr1_3:27536682-27536701 None:intergenic 35.0%
GATAAATTCAAAGCCCTTAG+TGG + chr1_3:27536764-27536783 None:intergenic 35.0%
AGAGCAGAGTAGCATAAAAA+TGG + chr1_3:27536855-27536874 None:intergenic 35.0%
GATAATCAAATGTGCATCTG+AGG + chr1_3:27536940-27536959 None:intergenic 35.0%
TGATACAGTCTGTTTGATGA+AGG - chr1_3:27537105-27537124 Msa0112550:intron 35.0%
CAGGGTGAAAATGTGTTATA+TGG + chr1_3:27537475-27537494 None:intergenic 35.0%
GGACTTCTATGAAATTTGCA+GGG + chr1_3:27537493-27537512 None:intergenic 35.0%
TGGACTTCTATGAAATTTGC+AGG + chr1_3:27537494-27537513 None:intergenic 35.0%
TACTTAAACTTCGATGCTTG+TGG - chr1_3:27537591-27537610 Msa0112550:intron 35.0%
GTTGTTCTATGTGGATATTG+GGG + chr1_3:27537691-27537710 None:intergenic 35.0%
GGTTGTTCTATGTGGATATT+GGG + chr1_3:27537692-27537711 None:intergenic 35.0%
TGGTTGTTCTATGTGGATAT+TGG + chr1_3:27537693-27537712 None:intergenic 35.0%
!! CTTGATTTGGTTGTTCTATG+TGG + chr1_3:27537700-27537719 None:intergenic 35.0%
AGTTCTGCTCTAACTTGATT+TGG + chr1_3:27537713-27537732 None:intergenic 35.0%
ATCAAGTTAGAGCAGAACTA+TGG - chr1_3:27537714-27537733 Msa0112550:intron 35.0%
AACTTGAACCAACGTAATGT+TGG + chr1_3:27538000-27538019 None:intergenic 35.0%
TCGTTAGTGAAGAAGGAAAA+AGG + chr1_3:27538228-27538247 None:intergenic 35.0%
!!! AGGCTTCAATATTTTTCAGG+TGG - chr1_3:27538324-27538343 Msa0112550:CDS 35.0%
ATTTATCTGTAACACGGGGA+AGG + chr1_3:27535496-27535515 None:intergenic 40.0%
TGGGATTTATCTGTAACACG+GGG + chr1_3:27535500-27535519 None:intergenic 40.0%
TTTCAGAACCGTACTCCAAA+CGG - chr1_3:27535549-27535568 Msa0112550:intron 40.0%
TCGTTGTTAAAAGACCGAAG+TGG + chr1_3:27535582-27535601 None:intergenic 40.0%
! AACGATTTCTTGGGGGATTT+TGG - chr1_3:27535597-27535616 Msa0112550:intron 40.0%
! ACGATTTCTTGGGGGATTTT+GGG - chr1_3:27535598-27535617 Msa0112550:intron 40.0%
! ATTGACTTTCCAACTCAGCT+GGG + chr1_3:27535638-27535657 None:intergenic 40.0%
! AATTGACTTTCCAACTCAGC+TGG + chr1_3:27535639-27535658 None:intergenic 40.0%
! GCTGAGTTGGAAAGTCAATT+CGG - chr1_3:27535639-27535658 Msa0112550:intron 40.0%
! GAGCAGAGATATTTGGGATA+AGG + chr1_3:27535704-27535723 None:intergenic 40.0%
TGCTCTTTCTCTTTCTCAAC+AGG - chr1_3:27535719-27535738 Msa0112550:intron 40.0%
TAGCATCATCTTGAGAAAGG+GGG + chr1_3:27535773-27535792 None:intergenic 40.0%
GGTTAGCATCATCTTGAGAA+AGG + chr1_3:27535776-27535795 None:intergenic 40.0%
CCTACCACAACCTAACAAAA+CGG - chr1_3:27536082-27536101 Msa0112550:intron 40.0%
CTACCACAACCTAACAAAAC+GGG - chr1_3:27536083-27536102 Msa0112550:intron 40.0%
GTTGTAGCTTAAAACCACCA+AGG - chr1_3:27536211-27536230 Msa0112550:CDS 40.0%
CTTACTTGCTCAATCAACCT+TGG + chr1_3:27536231-27536250 None:intergenic 40.0%
! CAAGGTTGATTGAGCAAGTA+AGG - chr1_3:27536229-27536248 Msa0112550:CDS 40.0%
!! CTGAAGAAGTGCTATTAGGT+TGG - chr1_3:27536329-27536348 Msa0112550:intron 40.0%
!! AAGAAGTGCTATTAGGTTGG+TGG - chr1_3:27536332-27536351 Msa0112550:intron 40.0%
ACATCGGATTAATTCTCGCT+CGG - chr1_3:27536443-27536462 Msa0112550:intron 40.0%
!!! CATTTATGCAGGTTTTGGGT+TGG - chr1_3:27537546-27537565 Msa0112550:intron 40.0%
TGGAGACTTGTGAATGATGA+AGG - chr1_3:27537566-27537585 Msa0112550:intron 40.0%
GAACTATGGACAACATCCAT+CGG - chr1_3:27537728-27537747 Msa0112550:intron 40.0%
TAGGAGGGAGTAGATAAGAA+GGG + chr1_3:27537771-27537790 None:intergenic 40.0%
ATAGGAGGGAGTAGATAAGA+AGG + chr1_3:27537772-27537791 None:intergenic 40.0%
CTGAGCTAAGAATGATAGGA+GGG + chr1_3:27537786-27537805 None:intergenic 40.0%
ACTGAGCTAAGAATGATAGG+AGG + chr1_3:27537787-27537806 None:intergenic 40.0%
TTACATTAACCGTGGTGAGA+TGG - chr1_3:27537853-27537872 Msa0112550:intron 40.0%
TTTCAATGTCCATCTCACCA+CGG + chr1_3:27537865-27537884 None:intergenic 40.0%
TGGTGAGATGGACATTGAAA+AGG - chr1_3:27537865-27537884 Msa0112550:intron 40.0%
GACTTAGATCCCTTAAGCAA+AGG - chr1_3:27538026-27538045 Msa0112550:intron 40.0%
! AAAGCCTCTAGTTTCTCTTG+TGG - chr1_3:27538053-27538072 Msa0112550:intron 40.0%
CAATCCACAAGAGAAACTAG+AGG + chr1_3:27538060-27538079 None:intergenic 40.0%
! AAGACCACAACTAGGTCTTT+TGG - chr1_3:27538100-27538119 Msa0112550:CDS 40.0%
GAATGAAGGGTTGAGTTTGG+TGG + chr1_3:27535526-27535545 None:intergenic 45.0%
TTCAGAACCGTACTCCAAAC+GGG - chr1_3:27535550-27535569 Msa0112550:intron 45.0%
! CGATTTCTTGGGGGATTTTG+GGG - chr1_3:27535599-27535618 Msa0112550:intron 45.0%
TTTCCAACTCAGCTGGGAAA+GGG + chr1_3:27535632-27535651 None:intergenic 45.0%
GGACTGTAAAGGTGAAATGG+GGG + chr1_3:27535751-27535770 None:intergenic 45.0%
GGGACTGTAAAGGTGAAATG+GGG + chr1_3:27535752-27535771 None:intergenic 45.0%
GGGGACTGTAAAGGTGAAAT+GGG + chr1_3:27535753-27535772 None:intergenic 45.0%
AGCATCATCTTGAGAAAGGG+GGG + chr1_3:27535772-27535791 None:intergenic 45.0%
!! CCGTTTTGTTAGGTTGTGGT+AGG + chr1_3:27536085-27536104 None:intergenic 45.0%
!! AGACCCGTTTTGTTAGGTTG+TGG + chr1_3:27536089-27536108 None:intergenic 45.0%
ACTTGCTCAATCAACCTTGG+TGG + chr1_3:27536228-27536247 None:intergenic 45.0%
AAGGAAGTGACTCCATTCCA+AGG + chr1_3:27536516-27536535 None:intergenic 45.0%
TGAAGAGAAAGAAGACGACG+AGG - chr1_3:27536633-27536652 Msa0112550:intron 45.0%
TCTACAGTTGTAGAGACTGC+AGG - chr1_3:27537659-27537678 Msa0112550:intron 45.0%
ACTGGTGTAATGCTTACCGA+TGG + chr1_3:27537747-27537766 None:intergenic 45.0%
! GGAGTAGATAAGAAGGGTAC+TGG + chr1_3:27537765-27537784 None:intergenic 45.0%
GCTTTGCAGCCTTTGCTTAA+GGG + chr1_3:27538038-27538057 None:intergenic 45.0%
TGTGACAGAAGACCACAACT+AGG - chr1_3:27538092-27538111 Msa0112550:CDS 45.0%
! ACCACAACTAGGTCTTTTGG+TGG - chr1_3:27538103-27538122 Msa0112550:CDS 45.0%
TCCACCAAAAGACCTAGTTG+TGG + chr1_3:27538107-27538126 None:intergenic 45.0%
AGACATCAGATCTTGTCCCA+AGG - chr1_3:27538394-27538413 Msa0112550:CDS 45.0%
TTGTCCCAAGGAAAAGAGCT+TGG - chr1_3:27538406-27538425 Msa0112550:CDS 45.0%
TGTCCCAAGGAAAAGAGCTT+GGG - chr1_3:27538407-27538426 Msa0112550:CDS 45.0%
! AAGCCCAAGCTCTTTTCCTT+GGG + chr1_3:27538413-27538432 None:intergenic 45.0%
! TAAGCCCAAGCTCTTTTCCT+TGG + chr1_3:27538414-27538433 None:intergenic 45.0%
!!! TTATTTTATTTTTACTAATA+AGG + chr1_3:27537255-27537274 None:intergenic 5.0%
!! GGGTTGAGTTTGGTGGTGTT+GGG + chr1_3:27535519-27535538 None:intergenic 50.0%
!! AGGGTTGAGTTTGGTGGTGT+TGG + chr1_3:27535520-27535539 None:intergenic 50.0%
! GAGAGAACCCGTTTGGAGTA+CGG + chr1_3:27535560-27535579 None:intergenic 50.0%
! CAAACGGGTTCTCTCCACTT+CGG - chr1_3:27535565-27535584 Msa0112550:intron 50.0%
CTTTCCAACTCAGCTGGGAA+AGG + chr1_3:27535633-27535652 None:intergenic 50.0%
GGGGGACTGTAAAGGTGAAA+TGG + chr1_3:27535754-27535773 None:intergenic 50.0%
TGAGAAAGGGGGGACTGTAA+AGG + chr1_3:27535762-27535781 None:intergenic 50.0%
!! CCTGACAGACCCGTTTTGTT+AGG + chr1_3:27536095-27536114 None:intergenic 50.0%
CCTAACAAAACGGGTCTGTC+AGG - chr1_3:27536092-27536111 Msa0112550:intron 50.0%
ACTCTTCTAAGCCGTGACCT+TGG - chr1_3:27536496-27536515 Msa0112550:intron 50.0%
TCTAAGCCGTGACCTTGGAA+TGG - chr1_3:27536501-27536520 Msa0112550:intron 50.0%
GTGACTCCATTCCAAGGTCA+CGG + chr1_3:27536510-27536529 None:intergenic 50.0%
GGCTTTGCAGCCTTTGCTTA+AGG + chr1_3:27538039-27538058 None:intergenic 50.0%
ATTCGTCATCTGCAAGCGAG+TGG - chr1_3:27538128-27538147 Msa0112550:CDS 50.0%
CGAAGTGGAGAGAACCCGTT+TGG + chr1_3:27535567-27535586 None:intergenic 55.0%
AGACCCTTTCCCAGCTGAGT+TGG - chr1_3:27535626-27535645 Msa0112550:intron 55.0%
CAAAACGGGTCTGTCAGGCG+AGG - chr1_3:27536097-27536116 Msa0112550:intron 60.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 27535465 27538434 27535465 ID=Msa0112550;Name=Msa0112550
chr1_3 mRNA 27535465 27538434 27535465 ID=Msa0112550-mRNA-1;Parent=Msa0112550;Name=Msa0112550-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|1|3|0|209
chr1_3 exon 27538090 27538434 27538090 ID=Msa0112550-mRNA-1:exon:9177;Parent=Msa0112550-mRNA-1
chr1_3 exon 27536150 27536342 27536150 ID=Msa0112550-mRNA-1:exon:9176;Parent=Msa0112550-mRNA-1
chr1_3 exon 27535465 27535556 27535465 ID=Msa0112550-mRNA-1:exon:9175;Parent=Msa0112550-mRNA-1
chr1_3 CDS 27538090 27538434 27538090 ID=Msa0112550-mRNA-1:cds;Parent=Msa0112550-mRNA-1
chr1_3 CDS 27536150 27536342 27536150 ID=Msa0112550-mRNA-1:cds;Parent=Msa0112550-mRNA-1
chr1_3 CDS 27535465 27535556 27535465 ID=Msa0112550-mRNA-1:cds;Parent=Msa0112550-mRNA-1
Gene Sequence

>Msa0112550

ATGGCTACAACCAAGCTTGTATTATTATCCTTCCCCGTGTTACAGATAAATCCCAACACCACCAAACTCAACCCTTCATTCAATTTTCAGAACCGTACTCCAAACGGGTTCTCTCCACTTCGGTCTTTTAACAACGATTTCTTGGGGGATTTTGGGGCAAGAGACCCTTTCCCAGCTGAGTTGGAAAGTCAATTCGGAGAGAAAGTTATAGGTAGTTATAACACCGAACACAAAATCCTTATCCCAAATATCTCTGCTCTTTCTCTTTCTCAACAGGAGTGTACCCCCATTTCACCTTTACAGTCCCCCCTTTCTCAAGATGATGCTAACCAGCTTATAAGAAAGGTTTTGGGTTGGAGACTTGTGAATGATGAAGGAGTACTTAAACTTCGATGCTTGTGGAAATTGAGAGATTTTAAATGCGGAGTTGAACTCATTAACAGAATCTCTACAGTTGTAGAGACTGCAGGACATTTCCCCAATATCCACATAGAACAACCAAATCAAGTTAGAGCAGAACTATGGACAACATCCATCGGTGGATTAAGTATGAATGATTTCATTGTAGCTGCAAAGATAGATGCAATACAGACATCAGATCTTGTCCCAAGGAAAAGAGCTTGGGCTTAA
Protein sequence

>Msa0112550

MATTKLVLLSFPVLQINPNTTKLNPSFNFQNRTPNGFSPLRSFNNDFLGDFGARDPFPAELESQFGEKVIGSYNTEHKILIPNISALSLSQQECTPISPLQSPLSQDDANQLIRKVLGWRLVNDEGVLKLRCLWKLRDFKCGVELINRISTVVETAGHFPNIHIEQPNQVRAELWTTSIGGLSMNDFIVAAKIDAIQTSDLVPRKRAWA*