Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0744180 | AET00817.1 | 98.592 | 71 | 1 | 0 | 1 | 71 | 1 | 71 | 1.63e-41 | 142 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0744180 | sp|Q9FG71|EMC89_ARATH | 72.603 | 73 | 18 | 2 | 1 | 71 | 3 | 75 | 1.21e-28 | 104 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0744180 | G7KEJ7 | 98.592 | 71 | 1 | 0 | 1 | 71 | 1 | 71 | 7.80e-42 | 142 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0744100 | Msa0744180 | 0.813482 | 2.503108e-51 | -8.615850e-47 |
| Msa0744180 | Msa0766670 | 0.803870 | 2.836430e-49 | -8.615850e-47 |
| Msa0744180 | Msa0788120 | 0.813889 | 2.036698e-51 | -8.615850e-47 |
| Msa0744180 | Msa0825270 | 0.822398 | 2.420260e-53 | -8.615850e-47 |
| Msa0744180 | Msa0826510 | 0.823817 | 1.129055e-53 | -8.615850e-47 |
| Msa0744180 | Msa0864420 | 0.810639 | 1.042745e-50 | -8.615850e-47 |
| Msa0744180 | Msa0865620 | 0.812845 | 3.454059e-51 | -8.615850e-47 |
| Msa0744180 | Msa1017510 | 0.813585 | 2.376007e-51 | -8.615850e-47 |
| Msa0744180 | Msa1034720 | 0.805808 | 1.116237e-49 | -8.615850e-47 |
| Msa0744180 | Msa1089270 | 0.828190 | 1.031697e-54 | -8.615850e-47 |
| Msa0744180 | Msa1127540 | 0.804135 | 2.498446e-49 | -8.615850e-47 |
| Msa0744180 | Msa1145240 | 0.802243 | 6.154733e-49 | -8.615850e-47 |
| Msa0744180 | Msa1162080 | 0.818522 | 1.875623e-52 | -8.615850e-47 |
| Msa0744180 | Msa1207730 | 0.825761 | 3.928896e-54 | -8.615850e-47 |
| Msa0744180 | Msa1364160 | 0.816978 | 4.183818e-52 | -8.615850e-47 |
| Msa0744180 | Msa1385810 | 0.819954 | 8.851863e-53 | -8.615850e-47 |
| Msa0744180 | Msa1409260 | 0.829664 | 4.536656e-55 | -8.615850e-47 |
| Msa0744180 | Msa1459910 | 0.817954 | 2.521759e-52 | -8.615850e-47 |
| Msa0127850 | Msa0744180 | 0.804505 | 2.092190e-49 | -8.615850e-47 |
| Msa0174000 | Msa0744180 | 0.810420 | 1.162834e-50 | -8.615850e-47 |
| Msa0198530 | Msa0744180 | 0.803575 | 3.264897e-49 | -8.615850e-47 |
| Msa0583500 | Msa0744180 | 0.816735 | 4.743764e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0744180 | MtrunA17_Chr5g0446831 | 98.592 | 71 | 1 | 0 | 1 | 71 | 1 | 71 | 7.25e-45 | 142 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0744180 | AT5G55940.1 | 72.603 | 73 | 18 | 2 | 1 | 71 | 3 | 75 | 1.23e-29 | 104 |
Find 24 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTATCTCTTTCTCTCTCTT+AGG | 0.188079 | 5_1:+64883447 | Msa0744180:CDS |
| ATTGGAGATCTCTCTTATTC+TGG | 0.256125 | 5_1:+64883420 | Msa0744180:CDS |
| AATCCCTCTTCTTCCTCAAT+TGG | 0.350775 | 5_1:+64883402 | Msa0744180:CDS |
| TTATCTCTTTCTCTCTCTTA+GGG | 0.358871 | 5_1:+64883448 | Msa0744180:CDS |
| GATTTCGACGGTGTCGTTAG+AGG | 0.416969 | 5_1:-64883350 | None:intergenic |
| GGGATTTGAGAGTGGAAAAG+AGG | 0.453984 | 5_1:-64883385 | None:intergenic |
| GGATTTGAGAGTGGAAAAGA+GGG | 0.454226 | 5_1:-64883384 | None:intergenic |
| ACGCCGTTGACGGCGGATGT+AGG | 0.461476 | 5_1:-64883307 | None:intergenic |
| CGCCGTTGACGGCGGATGTA+GGG | 0.461819 | 5_1:-64883306 | None:intergenic |
| GCCGATCAAGACGCCGTTGA+CGG | 0.463051 | 5_1:-64883317 | None:intergenic |
| GAGATCTCTCTTATTCTGGT+TGG | 0.469264 | 5_1:+64883424 | Msa0744180:CDS |
| GCCGTCAACGGCGTCTTGAT+CGG | 0.478430 | 5_1:+64883316 | Msa0744180:CDS |
| ATCTCCAATTGAGGAAGAAG+AGG | 0.512179 | 5_1:-64883406 | None:intergenic |
| CACCCTACATCCGCCGTCAA+CGG | 0.543335 | 5_1:+64883304 | Msa0744180:CDS |
| GAAGAAGAGGGATTTGAGAG+TGG | 0.543708 | 5_1:-64883393 | None:intergenic |
| GTGGAAAAGAGGGACGGCAT+CGG | 0.554411 | 5_1:-64883374 | None:intergenic |
| ATAAGAGAGATCTCCAATTG+AGG | 0.613544 | 5_1:-64883415 | None:intergenic |
| TTCGACGGTGTCGTTAGAGG+AGG | 0.616044 | 5_1:-64883347 | None:intergenic |
| GACGGCATCGGTGATTTCGA+CGG | 0.623195 | 5_1:-64883362 | None:intergenic |
| TCTCCAATTGAGGAAGAAGA+GGG | 0.626207 | 5_1:-64883405 | None:intergenic |
| GATCAAGACGCCGTTGACGG+CGG | 0.635153 | 5_1:-64883314 | None:intergenic |
| TTGAGAGTGGAAAAGAGGGA+CGG | 0.649379 | 5_1:-64883380 | None:intergenic |
| AAGTAACGATTTGAAGTACG+AGG | 0.660885 | 5_1:+64883237 | Msa0744180:CDS |
| TGTCGTTAGAGGAGGAGATG+CGG | 0.688753 | 5_1:-64883339 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGTAACGATTTGAAGTACG+AGG | + | chr5_1:64883237-64883256 | Msa0744180:CDS | 35.0% |
| ATAAGAGAGATCTCCAATTG+AGG | - | chr5_1:64883418-64883437 | None:intergenic | 35.0% | |
| ATTGGAGATCTCTCTTATTC+TGG | + | chr5_1:64883420-64883439 | Msa0744180:CDS | 35.0% | |
| GGATTTGAGAGTGGAAAAGA+GGG | - | chr5_1:64883387-64883406 | None:intergenic | 40.0% | |
| AATCCCTCTTCTTCCTCAAT+TGG | + | chr5_1:64883402-64883421 | Msa0744180:CDS | 40.0% | |
| TCTCCAATTGAGGAAGAAGA+GGG | - | chr5_1:64883408-64883427 | None:intergenic | 40.0% | |
| ATCTCCAATTGAGGAAGAAG+AGG | - | chr5_1:64883409-64883428 | None:intergenic | 40.0% | |
| GAGATCTCTCTTATTCTGGT+TGG | + | chr5_1:64883424-64883443 | Msa0744180:CDS | 40.0% | |
| TTGAGAGTGGAAAAGAGGGA+CGG | - | chr5_1:64883383-64883402 | None:intergenic | 45.0% | |
| GGGATTTGAGAGTGGAAAAG+AGG | - | chr5_1:64883388-64883407 | None:intergenic | 45.0% | |
| GAAGAAGAGGGATTTGAGAG+TGG | - | chr5_1:64883396-64883415 | None:intergenic | 45.0% | |
| TGTCGTTAGAGGAGGAGATG+CGG | - | chr5_1:64883342-64883361 | None:intergenic | 50.0% | |
| !! | GATTTCGACGGTGTCGTTAG+AGG | - | chr5_1:64883353-64883372 | None:intergenic | 50.0% |
| !! | TTCGACGGTGTCGTTAGAGG+AGG | - | chr5_1:64883350-64883369 | None:intergenic | 55.0% |
| !! | GACGGCATCGGTGATTTCGA+CGG | - | chr5_1:64883365-64883384 | None:intergenic | 55.0% |
| GTGGAAAAGAGGGACGGCAT+CGG | - | chr5_1:64883377-64883396 | None:intergenic | 55.0% | |
| CACCCTACATCCGCCGTCAA+CGG | + | chr5_1:64883304-64883323 | Msa0744180:CDS | 60.0% | |
| GATCAAGACGCCGTTGACGG+CGG | - | chr5_1:64883317-64883336 | None:intergenic | 60.0% | |
| GCCGTCAACGGCGTCTTGAT+CGG | + | chr5_1:64883316-64883335 | Msa0744180:CDS | 60.0% | |
| GCCGATCAAGACGCCGTTGA+CGG | - | chr5_1:64883320-64883339 | None:intergenic | 60.0% | |
| CGCCGTTGACGGCGGATGTA+GGG | - | chr5_1:64883309-64883328 | None:intergenic | 65.0% | |
| ACGCCGTTGACGGCGGATGT+AGG | - | chr5_1:64883310-64883329 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_1 | gene | 64883232 | 64883468 | 64883232 | ID=Msa0744180;Name=Msa0744180 |
| chr5_1 | mRNA | 64883232 | 64883468 | 64883232 | ID=Msa0744180-mRNA-1;Parent=Msa0744180;Name=Msa0744180-mRNA-1;_AED=0.30;_eAED=0.30;_QI=0|-1|0|1|-1|1|1|0|78 |
| chr5_1 | exon | 64883232 | 64883468 | 64883232 | ID=Msa0744180-mRNA-1:exon:19271;Parent=Msa0744180-mRNA-1 |
| chr5_1 | CDS | 64883232 | 64883468 | 64883232 | ID=Msa0744180-mRNA-1:cds;Parent=Msa0744180-mRNA-1 |
| Gene Sequence |
| Protein sequence |
