AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1364160


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1364160 MtrunA17_Chr2g0329891 99.468 188 1 0 1 188 1 188 1.10e-137 382
Msa1364160 MtrunA17_Chr8g0343071 75.419 179 44 0 10 188 4 182 1.96e-85 249
Msa1364160 MtrunA17_Chr1g0190881 61.749 183 69 1 7 188 3 185 5.31e-76 226
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1364160 AT1G72750.1 67.568 185 57 1 7 188 4 188 2.40e-76 227
Msa1364160 AT1G17530.1 62.366 186 64 2 5 188 6 187 1.67e-74 222
Msa1364160 AT1G17530.2 62.295 183 63 2 4 184 5 183 3.50e-73 219
Msa1364160 AT3G04800.1 52.459 183 86 1 7 188 6 188 7.84e-51 162

Find 72 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGCAGCAGGGCTTGGTACC+GGG 0.325322 tig0018106:-241841 Msa1364160:CDS
TTGGGAGCTGGTAGAGGTTT+CGG 0.328905 tig0018106:+242173 None:intergenic
TAGGGATTGTAGAGACGTTT+TGG 0.331643 tig0018106:+242217 None:intergenic
GTAGCCGCATCCGGTGTAAA+AGG 0.338122 tig0018106:+242084 None:intergenic
TTGGTACCGGGGCGTTGTAT+CGG 0.348837 tig0018106:-241829 Msa1364160:CDS
ACCTTTCTCGAACGATTTCA+CGG 0.355472 tig0018106:+242018 None:intergenic
GTGGCAGCAGGGCTTGGTAC+CGG 0.372617 tig0018106:-241842 Msa1364160:CDS
AAGAGATGTTCAGGTGAGGT+TGG 0.384154 tig0018106:+242154 None:intergenic
TCCTCGATGAAGAGATGTTC+AGG 0.386072 tig0018106:+242145 None:intergenic
GGCTACCTCGGCGGCGCCAT+TGG 0.387745 tig0018106:-242067 Msa1364160:CDS
TGGTACCGGGGCGTTGTATC+GGG 0.396607 tig0018106:-241828 Msa1364160:CDS
GTGAGATCAGCTGCTGTTGC+CGG 0.398096 tig0018106:-241794 Msa1364160:CDS
CGCATCCGGTGTAAAAGGTT+AGG 0.399415 tig0018106:+242089 None:intergenic
GCCGCCGAGGTAGCCGCATC+CGG 0.401744 tig0018106:+242075 None:intergenic
GGGAACCGCCTTGGTGTGAT+CGG 0.402790 tig0018106:-241932 Msa1364160:CDS
TCGGAGTTTGATTCGTGATC+GGG 0.411466 tig0018106:+242250 None:intergenic
CACAACACCACCAACAGCAC+CGG 0.411667 tig0018106:+241775 None:intergenic
GGCGGCGCCATTGGTGGCGC+CGG 0.412036 tig0018106:-242058 Msa1364160:CDS
AACAGTGTGGCAGCAGGGCT+TGG 0.418069 tig0018106:-241848 Msa1364160:CDS
GCTGTTGGTGGTGTTGTGGC+TGG 0.423812 tig0018106:-241770 Msa1364160:CDS
GGAACCGCCTTGGTGTGATC+GGG 0.429194 tig0018106:-241931 Msa1364160:CDS
TACCTCGGCGGCGCCATTGG+TGG 0.432819 tig0018106:-242064 Msa1364160:CDS
CGTTGTATCGGGCAGCGAGA+GGG 0.437956 tig0018106:-241817 Msa1364160:CDS
GTGAGGTTGGGAGCTGGTAG+AGG 0.443193 tig0018106:+242167 None:intergenic
GGATTGGTACTTCGAGGTCT+TGG 0.447073 tig0018106:+242194 None:intergenic
CTCAACGCAAACATCGTTCC+TGG 0.447403 tig0018106:-242120 Msa1364160:CDS
GTGGCTGGAGTGGCTATCGC+GGG 0.448335 tig0018106:-241755 Msa1364160:CDS
AGTGGCTATCGCGGGGAAGC+AGG 0.450298 tig0018106:-241747 Msa1364160:CDS
AAAGGTTAGGGTTTCTCCCC+AGG 0.463183 tig0018106:+242102 None:intergenic
GCCATTGGTGGCGCCGGCGT+AGG 0.463542 tig0018106:-242052 Msa1364160:CDS
GCTGCTGTTGCCGGTGCTGT+TGG 0.465020 tig0018106:-241785 Msa1364160:CDS
GCATCCGGTGTAAAAGGTTA+GGG 0.487745 tig0018106:+242090 None:intergenic
AACAAGATTCTGAATTCGTC+AGG 0.489468 tig0018106:-241974 Msa1364160:CDS
AGAGATGTTCAGGTGAGGTT+GGG 0.491614 tig0018106:+242155 None:intergenic
ATCGGAGTTTGATTCGTGAT+CGG 0.492854 tig0018106:+242249 None:intergenic
TGGTACTTCGAGGTCTTGGT+AGG 0.496614 tig0018106:+242198 None:intergenic
GCCGTGAAATCGTTCGAGAA+AGG 0.498953 tig0018106:-242019 Msa1364160:CDS
CGCCACCAATGGCGCCGCCG+AGG 0.504816 tig0018106:+242062 None:intergenic
GCTGTTGCCGGTGCTGTTGG+TGG 0.505954 tig0018106:-241782 Msa1364160:CDS
GGTTTCGGATTGGTACTTCG+AGG 0.508805 tig0018106:+242188 None:intergenic
GTTGTATCGGGCAGCGAGAG+GGG 0.520290 tig0018106:-241816 Msa1364160:CDS
ACCTACGCCGGCGCCACCAA+TGG 0.521262 tig0018106:+242051 None:intergenic
GCGTTGTATCGGGCAGCGAG+AGG 0.529365 tig0018106:-241818 Msa1364160:CDS
TCAACGCAAACATCGTTCCT+GGG 0.532503 tig0018106:-242119 Msa1364160:CDS
GGCAGCAGGGCTTGGTACCG+GGG 0.541292 tig0018106:-241840 Msa1364160:CDS
TGGAATTGAAAGCGGGATCA+CGG 0.541386 tig0018106:-241897 Msa1364160:CDS
CGGTGCTGTTGGTGGTGTTG+TGG 0.552191 tig0018106:-241774 Msa1364160:CDS
GATCACGGCGGCGAGAGATA+CGG 0.553451 tig0018106:-241882 Msa1364160:CDS
GTGATCGGGTTGTTATTCGC+TGG 0.562580 tig0018106:-241917 Msa1364160:CDS
GTTCAGGTGAGGTTGGGAGC+TGG 0.563872 tig0018106:+242161 None:intergenic
TGTGGCTGGAGTGGCTATCG+CGG 0.570123 tig0018106:-241756 Msa1364160:CDS
TCTGAATTCGTCAGGACACA+CGG 0.572872 tig0018106:-241966 Msa1364160:CDS
TGGTGGTGTTGTGGCTGGAG+TGG 0.577673 tig0018106:-241765 Msa1364160:CDS
GACACACGGGTCGGACGTGG+GGG 0.579942 tig0018106:-241952 Msa1364160:CDS
CGGAGTTTGATTCGTGATCG+GGG 0.580921 tig0018106:+242251 None:intergenic
GGTACTTCGAGGTCTTGGTA+GGG 0.582086 tig0018106:+242199 None:intergenic
TATTCGCTGGAATTGAAAGC+GGG 0.588545 tig0018106:-241904 Msa1364160:CDS
AGCTGGTAGAGGTTTCGGAT+TGG 0.594257 tig0018106:+242178 None:intergenic
ACCGGATGCGGCTACCTCGG+CGG 0.600899 tig0018106:-242076 Msa1364160:CDS
CTGAATTCGTCAGGACACAC+GGG 0.613772 tig0018106:-241965 Msa1364160:CDS
CGCTGCCCGATACAACGCCC+CGG 0.635857 tig0018106:+241823 None:intergenic
TTATTCGCTGGAATTGAAAG+CGG 0.641254 tig0018106:-241905 Msa1364160:CDS
GGAGTTTGATTCGTGATCGG+GGG 0.642744 tig0018106:+242252 None:intergenic
TGGCTGGAGTGGCTATCGCG+GGG 0.652115 tig0018106:-241754 Msa1364160:CDS
ATTCGTCAGGACACACGGGT+CGG 0.661517 tig0018106:-241961 Msa1364160:CDS
CAACGCAAACATCGTTCCTG+GGG 0.671475 tig0018106:-242118 Msa1364160:CDS
GGCTTCGACGAAACCTACGC+CGG 0.685279 tig0018106:+242039 None:intergenic
GATGAAGAGATGTTCAGGTG+AGG 0.693036 tig0018106:+242150 None:intergenic
ACCTGAACATCTCTTCATCG+AGG 0.710068 tig0018106:-242146 Msa1364160:CDS
ATAACAACCCGATCACACCA+AGG 0.726478 tig0018106:+241924 None:intergenic
AATTGAAAGCGGGATCACGG+CGG 0.736385 tig0018106:-241894 Msa1364160:CDS
ACAACCCGATCACACCAAGG+CGG 0.775839 tig0018106:+241927 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AACAAGATTCTGAATTCGTC+AGG - tig0018106:242012-242031 Msa1364160:CDS 35.0%
TTATTCGCTGGAATTGAAAG+CGG - tig0018106:242081-242100 Msa1364160:CDS 35.0%
! ATCGGAGTTTGATTCGTGAT+CGG + tig0018106:241740-241759 None:intergenic 40.0%
!! GATTGTAGAGACGTTTTGGA+GGG + tig0018106:241768-241787 None:intergenic 40.0%
! TAGGGATTGTAGAGACGTTT+TGG + tig0018106:241772-241791 None:intergenic 40.0%
! GAAACCCTAACCTTTTACAC+CGG - tig0018106:241892-241911 Msa1364160:CDS 40.0%
ACCTTTCTCGAACGATTTCA+CGG + tig0018106:241971-241990 None:intergenic 40.0%
TATTCGCTGGAATTGAAAGC+GGG - tig0018106:242082-242101 Msa1364160:CDS 40.0%
! TCGGAGTTTGATTCGTGATC+GGG + tig0018106:241739-241758 None:intergenic 45.0%
!!! ACGTTTTGGAGGGTTGGAAT+CGG + tig0018106:241758-241777 None:intergenic 45.0%
! GGATTGTAGAGACGTTTTGG+AGG + tig0018106:241769-241788 None:intergenic 45.0%
AGAGATGTTCAGGTGAGGTT+GGG + tig0018106:241834-241853 None:intergenic 45.0%
AAGAGATGTTCAGGTGAGGT+TGG + tig0018106:241835-241854 None:intergenic 45.0%
GATGAAGAGATGTTCAGGTG+AGG + tig0018106:241839-241858 None:intergenic 45.0%
ACCTGAACATCTCTTCATCG+AGG - tig0018106:241840-241859 Msa1364160:CDS 45.0%
TCCTCGATGAAGAGATGTTC+AGG + tig0018106:241844-241863 None:intergenic 45.0%
TCAACGCAAACATCGTTCCT+GGG - tig0018106:241867-241886 Msa1364160:CDS 45.0%
GCATCCGGTGTAAAAGGTTA+GGG + tig0018106:241899-241918 None:intergenic 45.0%
! CTAACCTTTTACACCGGATG+CGG - tig0018106:241898-241917 Msa1364160:CDS 45.0%
TCTGAATTCGTCAGGACACA+CGG - tig0018106:242020-242039 Msa1364160:CDS 45.0%
ATAACAACCCGATCACACCA+AGG + tig0018106:242065-242084 None:intergenic 45.0%
TGGAATTGAAAGCGGGATCA+CGG - tig0018106:242089-242108 Msa1364160:CDS 45.0%
! GGACGATGTTTTCAACAGTG+TGG - tig0018106:242125-242144 Msa1364160:CDS 45.0%
! TTTTCAACAGTGTGGCAGCA+GGG - tig0018106:242133-242152 Msa1364160:CDS 45.0%
! GGAGTTTGATTCGTGATCGG+GGG + tig0018106:241737-241756 None:intergenic 50.0%
! CGGAGTTTGATTCGTGATCG+GGG + tig0018106:241738-241757 None:intergenic 50.0%
!!! GTAGAGACGTTTTGGAGGGT+TGG + tig0018106:241764-241783 None:intergenic 50.0%
GGTACTTCGAGGTCTTGGTA+GGG + tig0018106:241790-241809 None:intergenic 50.0%
TGGTACTTCGAGGTCTTGGT+AGG + tig0018106:241791-241810 None:intergenic 50.0%
! GGATTGGTACTTCGAGGTCT+TGG + tig0018106:241795-241814 None:intergenic 50.0%
!! GGTTTCGGATTGGTACTTCG+AGG + tig0018106:241801-241820 None:intergenic 50.0%
AGCTGGTAGAGGTTTCGGAT+TGG + tig0018106:241811-241830 None:intergenic 50.0%
TTGGGAGCTGGTAGAGGTTT+CGG + tig0018106:241816-241835 None:intergenic 50.0%
CTCAACGCAAACATCGTTCC+TGG - tig0018106:241866-241885 Msa1364160:CDS 50.0%
CAACGCAAACATCGTTCCTG+GGG - tig0018106:241868-241887 Msa1364160:CDS 50.0%
AAAGGTTAGGGTTTCTCCCC+AGG + tig0018106:241887-241906 None:intergenic 50.0%
CGCATCCGGTGTAAAAGGTT+AGG + tig0018106:241900-241919 None:intergenic 50.0%
GCCGTGAAATCGTTCGAGAA+AGG - tig0018106:241967-241986 Msa1364160:CDS 50.0%
CTGAATTCGTCAGGACACAC+GGG - tig0018106:242021-242040 Msa1364160:CDS 50.0%
! GTGATCGGGTTGTTATTCGC+TGG - tig0018106:242069-242088 Msa1364160:CDS 50.0%
AATTGAAAGCGGGATCACGG+CGG - tig0018106:242092-242111 Msa1364160:CDS 50.0%
! GTTTTCAACAGTGTGGCAGC+AGG - tig0018106:242132-242151 Msa1364160:CDS 50.0%
GTAGCCGCATCCGGTGTAAA+AGG + tig0018106:241905-241924 None:intergenic 55.0%
ATTCGTCAGGACACACGGGT+CGG - tig0018106:242025-242044 Msa1364160:CDS 55.0%
ACAACCCGATCACACCAAGG+CGG + tig0018106:242062-242081 None:intergenic 55.0%
!! TTGGTACCGGGGCGTTGTAT+CGG - tig0018106:242157-242176 Msa1364160:CDS 55.0%
GTGAGATCAGCTGCTGTTGC+CGG - tig0018106:242192-242211 Msa1364160:CDS 55.0%
CACAACACCACCAACAGCAC+CGG + tig0018106:242214-242233 None:intergenic 55.0%
GTGAGGTTGGGAGCTGGTAG+AGG + tig0018106:241822-241841 None:intergenic 60.0%
GTTCAGGTGAGGTTGGGAGC+TGG + tig0018106:241828-241847 None:intergenic 60.0%
TACACCGGATGCGGCTACCT+CGG - tig0018106:241907-241926 Msa1364160:CDS 60.0%
GGCTTCGACGAAACCTACGC+CGG + tig0018106:241950-241969 None:intergenic 60.0%
! GGGAACCGCCTTGGTGTGAT+CGG - tig0018106:242054-242073 Msa1364160:CDS 60.0%
! GGAACCGCCTTGGTGTGATC+GGG - tig0018106:242055-242074 Msa1364160:CDS 60.0%
GATCACGGCGGCGAGAGATA+CGG - tig0018106:242104-242123 Msa1364160:CDS 60.0%
AACAGTGTGGCAGCAGGGCT+TGG - tig0018106:242138-242157 Msa1364160:CDS 60.0%
!! TGGTACCGGGGCGTTGTATC+GGG - tig0018106:242158-242177 Msa1364160:CDS 60.0%
CGTTGTATCGGGCAGCGAGA+GGG - tig0018106:242169-242188 Msa1364160:CDS 60.0%
GTTGTATCGGGCAGCGAGAG+GGG - tig0018106:242170-242189 Msa1364160:CDS 60.0%
!! CGGTGCTGTTGGTGGTGTTG+TGG - tig0018106:242212-242231 Msa1364160:CDS 60.0%
!! GCTGTTGGTGGTGTTGTGGC+TGG - tig0018106:242216-242235 Msa1364160:CDS 60.0%
!! TGGTGGTGTTGTGGCTGGAG+TGG - tig0018106:242221-242240 Msa1364160:CDS 60.0%
TGTGGCTGGAGTGGCTATCG+CGG - tig0018106:242230-242249 Msa1364160:CDS 60.0%
AGGACACACGGGTCGGACGT+GGG - tig0018106:242032-242051 Msa1364160:CDS 65.0%
! GTGGCAGCAGGGCTTGGTAC+CGG - tig0018106:242144-242163 Msa1364160:CDS 65.0%
! TGGCAGCAGGGCTTGGTACC+GGG - tig0018106:242145-242164 Msa1364160:CDS 65.0%
GCGTTGTATCGGGCAGCGAG+AGG - tig0018106:242168-242187 Msa1364160:CDS 65.0%
!! GCTGCTGTTGCCGGTGCTGT+TGG - tig0018106:242201-242220 Msa1364160:CDS 65.0%
!! GCTGTTGCCGGTGCTGTTGG+TGG - tig0018106:242204-242223 Msa1364160:CDS 65.0%
GTGGCTGGAGTGGCTATCGC+GGG - tig0018106:242231-242250 Msa1364160:CDS 65.0%
TGGCTGGAGTGGCTATCGCG+GGG - tig0018106:242232-242251 Msa1364160:CDS 65.0%
AGTGGCTATCGCGGGGAAGC+AGG - tig0018106:242239-242258 Msa1364160:CDS 65.0%
ACCGGATGCGGCTACCTCGG+CGG - tig0018106:241910-241929 Msa1364160:CDS 70.0%
TACCTCGGCGGCGCCATTGG+TGG - tig0018106:241922-241941 Msa1364160:CDS 70.0%
ACCTACGCCGGCGCCACCAA+TGG + tig0018106:241938-241957 None:intergenic 70.0%
CAGGACACACGGGTCGGACG+TGG - tig0018106:242031-242050 Msa1364160:CDS 70.0%
GGACACACGGGTCGGACGTG+GGG - tig0018106:242033-242052 Msa1364160:CDS 70.0%
GACACACGGGTCGGACGTGG+GGG - tig0018106:242034-242053 Msa1364160:CDS 70.0%
! GGCAGCAGGGCTTGGTACCG+GGG - tig0018106:242146-242165 Msa1364160:CDS 70.0%
CGCTGCCCGATACAACGCCC+CGG + tig0018106:242166-242185 None:intergenic 70.0%
GCCGCCGAGGTAGCCGCATC+CGG + tig0018106:241914-241933 None:intergenic 75.0%
GGCTACCTCGGCGGCGCCAT+TGG - tig0018106:241919-241938 Msa1364160:CDS 75.0%
!! GCCATTGGTGGCGCCGGCGT+AGG - tig0018106:241934-241953 Msa1364160:CDS 75.0%
CGGACGTGGGGGAACCGCCT+TGG - tig0018106:242045-242064 Msa1364160:CDS 75.0%
! CGCCACCAATGGCGCCGCCG+AGG + tig0018106:241927-241946 None:intergenic 80.0%
!!! GGCGGCGCCATTGGTGGCGC+CGG - tig0018106:241928-241947 Msa1364160:CDS 80.0%
Chromosome Type Strat End Strand Name
tig0018106 gene 241721 242287 241721 ID=Msa1364160;Name=Msa1364160
tig0018106 mRNA 241721 242287 241721 ID=Msa1364160-mRNA-1;Parent=Msa1364160;Name=Msa1364160-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|188
tig0018106 exon 241721 242287 241721 ID=Msa1364160-mRNA-1:exon:13853;Parent=Msa1364160-mRNA-1
tig0018106 CDS 241721 242287 241721 ID=Msa1364160-mRNA-1:cds;Parent=Msa1364160-mRNA-1
Gene Sequence

>Msa1364160

ATGGCGTTTCACTCCCCCGATCACGAATCAAACTCCGATTCCAACCCTCCAAAACGTCTCTACAATCCCTACCAAGACCTCGAAGTACCAATCCGAAACCTCTACCAGCTCCCAACCTCACCTGAACATCTCTTCATCGAGGAAGCTCAACGCAAACATCGTTCCTGGGGAGAAACCCTAACCTTTTACACCGGATGCGGCTACCTCGGCGGCGCCATTGGTGGCGCCGGCGTAGGTTTCGTCGAAGCCGTGAAATCGTTCGAGAAAGGTGATACTAATAAGCTCAGACTCAACAAGATTCTGAATTCGTCAGGACACACGGGTCGGACGTGGGGGAACCGCCTTGGTGTGATCGGGTTGTTATTCGCTGGAATTGAAAGCGGGATCACGGCGGCGAGAGATACGGACGATGTTTTCAACAGTGTGGCAGCAGGGCTTGGTACCGGGGCGTTGTATCGGGCAGCGAGAGGGGTGAGATCAGCTGCTGTTGCCGGTGCTGTTGGTGGTGTTGTGGCTGGAGTGGCTATCGCGGGGAAGCAGGCTTTGAAACGCTATGTGCCTATATGA

Protein sequence

>Msa1364160

MAFHSPDHESNSDSNPPKRLYNPYQDLEVPIRNLYQLPTSPEHLFIEEAQRKHRSWGETLTFYTGCGYLGGAIGGAGVGFVEAVKSFEKGDTNKLRLNKILNSSGHTGRTWGNRLGVIGLLFAGIESGITAARDTDDVFNSVAAGLGTGALYRAARGVRSAAVAGAVGGVVAGVAIAGKQALKRYVPI*