AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0583500

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0583500 MtrunA17_Chr4g0030911 78.333 240 5 3 1 240 1 193 4.24e-125 355
Msa0583500 MtrunA17_Chr1g0182321 76.271 236 9 3 1 236 1 189 1.59e-121 346
Msa0583500 MtrunA17_Chr1g0182301 75.847 236 10 3 1 236 1 189 8.19e-121 344
Msa0583500 MtrunA17_Chr7g0263071 76.087 230 8 3 11 240 11 193 5.18e-114 327
Msa0583500 MtrunA17_Chr1g0212521 71.371 248 24 3 11 258 11 211 4.46e-113 325
Msa0583500 MtrunA17_Chr2g0327911 64.655 232 34 4 1 231 1 185 6.35e-94 276
Msa0583500 MtrunA17_Chr7g0250531 51.948 231 62 5 11 240 4 186 8.44e-70 214
Msa0583500 MtrunA17_Chr4g0055651 50.000 228 66 4 11 238 4 183 2.04e-68 211
Msa0583500 MtrunA17_Chr8g0335471 50.431 232 65 5 11 240 4 187 5.74e-67 207
Msa0583500 MtrunA17_Chr2g0276931 47.945 219 66 4 11 229 5 175 8.02e-62 194
Msa0583500 MtrunA17_Chr4g0032201 45.930 172 72 3 7 178 4 154 1.33e-46 155
Msa0583500 MtrunA17_Chr2g0290891 40.639 219 82 3 12 230 3 173 1.03e-45 153
Msa0583500 MtrunA17_Chr6g0458081 40.183 219 83 3 12 230 3 173 1.18e-45 153
Msa0583500 MtrunA17_Chr3g0112681 37.313 268 102 6 11 278 8 209 1.62e-45 153
Msa0583500 MtrunA17_Chr2g0276941 45.833 168 70 3 11 178 11 157 9.40e-45 151
Msa0583500 MtrunA17_Chr1g0189361 46.386 166 68 3 13 178 12 156 9.67e-45 151
Msa0583500 MtrunA17_Chr2g0315551 47.674 172 69 3 7 178 5 155 1.12e-44 150
Msa0583500 MtrunA17_Chr2g0319951 44.767 172 74 2 7 178 5 155 6.00e-44 149
Msa0583500 MtrunA17_Chr4g0040781 47.093 172 70 3 7 178 5 155 2.16e-43 147
Msa0583500 MtrunA17_Chr3g0123821 42.690 171 77 3 8 178 9 158 2.63e-43 147
Msa0583500 MtrunA17_Chr2g0283131 47.093 172 70 3 7 178 5 155 3.21e-43 147
Msa0583500 MtrunA17_Chr4g0046261 45.930 172 72 3 7 178 5 155 8.08e-43 146
Msa0583500 MtrunA17_Chr8g0381841 43.976 166 72 3 13 178 15 159 8.90e-43 146
Msa0583500 MtrunA17_Chr8g0349711 38.095 231 90 5 14 239 5 187 1.49e-42 145
Msa0583500 MtrunA17_Chr1g0180291 48.503 167 64 4 12 177 11 156 3.33e-42 144
Msa0583500 MtrunA17_Chr7g0265001 40.741 216 80 4 12 226 13 181 9.52e-42 143
Msa0583500 MtrunA17_Chr3g0130271 45.238 168 71 3 11 177 8 155 1.94e-41 142
Msa0583500 MtrunA17_Chr8g0384311 43.931 173 75 4 6 177 4 155 4.81e-41 141
Msa0583500 MtrunA17_Chr8g0349681 37.500 224 92 3 9 232 378 553 1.22e-40 148
Msa0583500 MtrunA17_Chr8g0349681 36.323 223 90 4 12 230 172 346 3.14e-36 136
Msa0583500 MtrunA17_Chr8g0349681 42.683 164 73 2 14 177 12 154 4.60e-35 133
Msa0583500 MtrunA17_Chr7g0234201 44.242 165 71 3 14 178 12 155 1.96e-40 139
Msa0583500 MtrunA17_Chr1g0159481 40.465 215 79 6 11 224 13 179 2.16e-40 139
Msa0583500 MtrunA17_Chr8g0351861 43.373 166 73 2 13 178 14 158 1.38e-38 135
Msa0583500 MtrunA17_Chr2g0325091 44.643 168 72 3 11 178 7 153 1.38e-38 135
Msa0583500 MtrunA17_Chr8g0346421 41.566 166 76 2 13 178 24 168 1.47e-38 135
Msa0583500 MtrunA17_Chr1g0197331 44.134 179 78 4 1 177 1 159 2.02e-38 134
Msa0583500 MtrunA17_Chr1g0174281 42.169 166 75 2 13 178 13 157 4.08e-38 134
Msa0583500 MtrunA17_Chr7g0214241 41.212 165 76 2 14 178 14 157 5.89e-38 133
Msa0583500 MtrunA17_Chr7g0275991 39.759 166 79 2 13 178 18 162 1.99e-37 132
Msa0583500 MtrunA17_Chr7g0231131 40.361 166 78 2 13 178 13 157 3.79e-36 129
Msa0583500 MtrunA17_Chr6g0482721 36.792 212 86 4 13 224 10 173 4.43e-35 125
Msa0583500 MtrunA17_Chr8g0392931 38.462 208 80 3 13 220 12 171 1.01e-33 122
Msa0583500 MtrunA17_Chr1g0199331 34.434 212 90 4 16 226 32 195 3.66e-33 120
Msa0583500 MtrunA17_Chr8g0390561 33.180 217 97 3 8 224 2 170 8.61e-33 119
Msa0583500 MtrunA17_Chr3g0109721 31.250 224 105 4 17 240 12 186 9.91e-31 114
Msa0583500 MtrunA17_Chr5g0433561 31.250 224 105 4 17 240 12 186 1.13e-30 114
Msa0583500 MtrunA17_Chr8g0382221 38.037 163 78 3 17 178 11 151 2.48e-30 113
Msa0583500 MtrunA17_Chr3g0123971 37.423 163 79 3 17 178 11 151 1.64e-28 108
Msa0583500 MtrunA17_Chr4g0055081 35.838 173 86 4 5 177 3 150 1.75e-27 105
Msa0583500 MtrunA17_Chr4g0034871 33.778 225 92 8 5 223 2 175 1.80e-27 105
Msa0583500 MtrunA17_Chr5g0400131 36.207 174 86 4 5 178 3 151 2.48e-27 105
Msa0583500 MtrunA17_Chr6g0466301 31.308 214 94 4 15 222 8 174 6.32e-25 99.0
Msa0583500 MtrunA17_Chr6g0465491 31.308 214 94 4 15 222 8 174 6.32e-25 99.0
Msa0583500 MtrunA17_Chr8g0383261 30.357 224 103 4 15 232 12 188 1.82e-24 97.8
Msa0583500 MtrunA17_Chr2g0331691 35.088 171 83 5 15 178 10 159 2.23e-23 95.1
Msa0583500 MtrunA17_Chr1g0201121 31.905 210 98 3 15 222 8 174 2.54e-23 94.7
Msa0583500 MtrunA17_Chr4g0004661 31.944 216 92 6 15 223 64 231 3.82e-23 95.9
Msa0583500 MtrunA17_Chr1g0178291 37.126 167 75 5 18 182 431 569 7.64e-18 83.6
Msa0583500 MtrunA17_Chr4g0046211 30.070 143 79 3 15 157 6 127 9.89e-18 78.2
Msa0583500 MtrunA17_Chr4g0037021 28.638 213 112 6 15 226 6 179 1.42e-17 79.0
Msa0583500 MtrunA17_Chr2g0325101 28.079 203 108 5 15 217 6 170 1.44e-17 79.0
Msa0583500 MtrunA17_Chr8g0371501 28.931 159 85 4 15 166 6 143 4.81e-17 77.8
Msa0583500 MtrunA17_Chr5g0405791 28.931 159 85 4 15 166 6 143 8.49e-17 77.0
Msa0583500 MtrunA17_Chr3g0117931 26.882 186 98 5 15 183 8 172 1.69e-16 76.6
Msa0583500 MtrunA17_Chr6g0466161 29.864 221 109 6 32 250 7 183 2.78e-16 75.9
Msa0583500 MtrunA17_Chr6g0465471 31.088 193 88 5 32 222 7 156 5.62e-16 75.1
Msa0583500 MtrunA17_Chr6g0486041 28.571 203 107 7 15 217 6 170 2.90e-15 73.2
Msa0583500 MtrunA17_Chr3g0138121 25.455 220 112 7 5 224 4 171 5.19e-15 72.4
Msa0583500 MtrunA17_Chr3g0138101 25.455 220 112 7 5 224 4 171 6.08e-15 72.4
Msa0583500 MtrunA17_Chr3g0138111 25.455 220 112 7 5 224 4 171 6.85e-15 72.4
Msa0583500 MtrunA17_Chr6g0466431 35.165 91 50 1 15 105 8 89 1.04e-13 65.9
Msa0583500 MtrunA17_Chr6g0465611 35.165 91 50 1 15 105 8 89 1.04e-13 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0583500 AT5G59840.1 71.371 248 24 3 11 258 11 211 1.43e-114 329
Msa0583500 AT3G46060.2 70.968 248 25 3 11 258 11 211 2.73e-114 328
Msa0583500 AT3G46060.3 70.968 248 25 3 11 258 11 211 2.73e-114 328
Msa0583500 AT3G46060.1 70.968 248 25 3 11 258 11 211 2.73e-114 328
Msa0583500 AT3G53610.2 70.968 248 25 3 11 258 11 211 4.30e-113 325
Msa0583500 AT3G53610.3 70.968 248 25 3 11 258 11 211 4.30e-113 325
Msa0583500 AT3G53610.1 70.968 248 25 3 11 258 11 211 4.30e-113 325
Msa0583500 AT5G03520.1 72.458 236 18 3 1 236 1 189 2.12e-106 308
Msa0583500 AT3G09900.1 71.610 236 20 3 1 236 1 189 4.49e-105 305
Msa0583500 AT5G03520.2 67.914 187 13 3 50 236 40 179 2.39e-79 239
Msa0583500 AT1G02130.1 51.724 232 62 5 11 240 4 187 1.73e-71 219
Msa0583500 AT4G17530.1 51.598 219 58 4 11 229 4 174 4.87e-66 205
Msa0583500 AT5G47200.1 51.598 219 58 4 11 229 4 174 8.66e-66 204
Msa0583500 AT3G11730.1 50.224 223 63 4 11 233 4 178 6.52e-65 202
Msa0583500 AT4G35860.1 41.096 219 81 3 12 230 3 173 3.68e-48 160
Msa0583500 AT4G17160.1 40.639 219 82 3 12 230 3 173 2.19e-47 157
Msa0583500 AT4G17170.1 40.639 219 82 3 12 230 3 173 2.64e-46 155
Msa0583500 AT5G59150.1 45.349 172 73 3 7 178 4 154 3.29e-46 155
Msa0583500 AT1G16920.1 47.093 172 70 3 7 178 5 155 9.67e-46 154
Msa0583500 AT3G46830.1 45.349 172 73 3 7 178 4 154 1.60e-45 153
Msa0583500 AT3G46830.2 45.349 172 73 3 7 178 4 154 1.60e-45 153
Msa0583500 AT1G09630.1 40.654 214 100 5 7 215 4 195 2.25e-45 153
Msa0583500 AT1G07410.1 44.643 168 72 3 11 178 8 154 8.81e-45 151
Msa0583500 AT4G18430.1 46.512 172 71 3 7 178 5 155 9.77e-44 149
Msa0583500 AT1G06400.1 44.186 172 75 2 7 178 5 155 9.43e-43 146
Msa0583500 AT3G07410.1 45.783 166 69 2 13 178 10 154 1.07e-42 146
Msa0583500 AT3G15060.1 46.512 172 71 3 7 178 5 155 1.08e-42 146
Msa0583500 AT4G39990.1 43.976 166 72 3 13 178 15 159 1.56e-42 145
Msa0583500 AT1G05810.1 43.373 166 73 2 13 178 10 154 3.94e-42 144
Msa0583500 AT5G60860.1 45.930 172 72 3 7 178 5 155 4.95e-42 144
Msa0583500 AT5G47520.1 45.783 166 69 3 13 178 12 156 5.08e-42 144
Msa0583500 AT1G28550.1 45.930 172 72 3 7 178 5 155 5.26e-42 144
Msa0583500 AT1G05810.2 43.976 166 72 3 13 178 56 200 6.69e-42 145
Msa0583500 AT5G45750.1 44.767 172 74 3 7 178 5 155 9.15e-42 143
Msa0583500 AT4G18800.1 45.349 172 73 3 7 178 5 155 1.03e-41 143
Msa0583500 AT2G31680.1 42.771 166 74 2 13 178 10 154 3.49e-41 142
Msa0583500 AT2G43130.1 43.373 166 73 3 13 178 10 154 4.60e-41 141
Msa0583500 AT5G03530.2 37.743 257 99 7 8 261 6 204 6.52e-41 141
Msa0583500 AT5G03530.1 37.743 257 99 7 8 261 6 204 6.52e-41 141
Msa0583500 AT1G01200.1 37.395 238 102 4 13 250 26 216 8.49e-41 142
Msa0583500 AT5G47960.1 43.976 166 72 2 13 178 13 157 8.88e-41 141
Msa0583500 AT2G33870.1 44.767 172 74 3 7 178 5 155 1.32e-40 140
Msa0583500 AT5G65270.1 41.566 166 76 3 13 178 15 159 3.91e-40 139
Msa0583500 AT1G43890.3 39.906 213 79 5 6 217 4 168 8.00e-40 138
Msa0583500 AT1G43890.1 39.906 213 79 5 6 217 4 168 8.00e-40 138
Msa0583500 AT1G43890.2 39.906 213 79 5 6 217 4 168 8.00e-40 138
Msa0583500 AT3G12160.1 42.169 166 75 2 13 178 13 157 6.48e-39 136
Msa0583500 AT1G18200.1 38.889 216 84 3 13 228 11 178 8.54e-38 134
Msa0583500 AT3G54840.2 41.243 177 79 4 3 178 24 176 1.63e-37 132
Msa0583500 AT3G54840.1 41.243 177 79 4 3 178 24 176 2.14e-37 132
Msa0583500 AT1G73640.1 37.447 235 93 6 13 241 11 197 1.02e-36 131
Msa0583500 AT3G54840.3 41.975 162 72 3 18 178 2 142 3.04e-36 127
Msa0583500 AT3G09910.5 39.732 224 86 7 8 230 6 181 1.88e-33 121
Msa0583500 AT3G09910.4 39.732 224 86 7 8 230 6 181 1.88e-33 121
Msa0583500 AT3G09910.3 41.919 198 83 7 8 194 6 182 1.96e-33 121
Msa0583500 AT3G09910.1 41.919 198 83 7 8 194 6 182 1.96e-33 121
Msa0583500 AT3G09910.2 41.919 198 83 7 8 194 6 182 1.96e-33 121
Msa0583500 AT4G19640.1 32.258 217 98 4 17 233 12 179 3.64e-31 115
Msa0583500 AT4G39890.1 33.981 206 87 5 17 221 11 168 3.44e-29 110
Msa0583500 AT4G35860.2 38.506 174 59 4 57 230 2 127 7.24e-29 108
Msa0583500 AT2G22290.1 37.654 162 80 3 17 178 11 151 1.06e-28 109
Msa0583500 AT5G45130.1 29.954 217 103 4 17 233 12 179 2.88e-28 108
Msa0583500 AT3G18820.1 32.889 225 94 6 5 223 2 175 6.16e-28 107
Msa0583500 AT5G10260.1 37.037 162 81 3 17 178 11 151 1.17e-27 106
Msa0583500 AT5G59150.2 43.443 122 48 3 57 178 3 103 4.48e-27 103
Msa0583500 AT5G45130.2 29.302 215 103 4 19 233 22 187 1.18e-26 103
Msa0583500 AT2G44610.1 35.632 174 87 4 5 178 3 151 2.73e-26 103
Msa0583500 AT5G64990.1 35.802 162 83 2 17 178 9 149 8.41e-26 101
Msa0583500 AT1G52280.1 36.471 170 82 4 15 178 8 157 1.57e-25 100
Msa0583500 AT1G49300.2 35.326 184 89 6 5 182 2 161 2.65e-25 100
Msa0583500 AT1G49300.1 35.326 184 89 6 5 182 2 161 2.65e-25 100
Msa0583500 AT4G19640.2 34.043 141 73 1 17 157 12 132 8.30e-25 97.4
Msa0583500 AT1G22740.1 37.059 170 81 5 15 178 8 157 1.50e-24 98.2
Msa0583500 AT4G19640.3 34.043 141 73 1 17 157 12 132 2.46e-24 97.4
Msa0583500 AT4G09720.1 33.645 214 89 6 15 222 8 174 2.74e-24 97.8
Msa0583500 AT2G21880.1 30.189 212 99 5 15 222 9 175 3.32e-24 97.8
Msa0583500 AT4G09720.4 33.962 212 96 5 15 222 8 179 4.38e-24 97.4
Msa0583500 AT3G16100.1 34.706 170 85 3 15 178 8 157 7.30e-24 96.7
Msa0583500 AT4G09720.3 32.857 210 107 3 15 222 8 185 9.26e-24 96.7
Msa0583500 AT5G64990.2 34.320 169 83 3 17 178 9 156 4.14e-23 94.7
Msa0583500 AT5G39620.1 30.093 216 92 5 16 222 6 171 6.97e-22 91.3
Msa0583500 AT4G35020.1 29.557 203 105 5 15 217 6 170 3.54e-19 84.0
Msa0583500 AT4G35020.2 29.557 203 105 5 15 217 6 170 3.54e-19 84.0
Msa0583500 AT4G35020.3 29.557 203 105 5 15 217 6 170 3.54e-19 84.0
Msa0583500 AT5G62880.1 27.570 214 117 5 15 228 8 183 7.17e-18 80.9
Msa0583500 AT3G48040.2 26.842 190 104 5 15 190 8 176 1.30e-17 79.0
Msa0583500 AT5G45970.1 27.586 203 109 5 15 217 6 170 1.87e-17 79.3
Msa0583500 AT2G17800.2 28.495 186 95 6 15 183 6 170 2.92e-17 78.6
Msa0583500 AT2G17800.1 28.495 186 95 6 15 183 6 170 2.92e-17 78.6
Msa0583500 AT3G51300.1 28.144 167 90 5 15 172 6 151 5.82e-17 77.8
Msa0583500 AT4G35950.1 28.495 186 95 6 15 183 6 170 7.58e-17 77.4
Msa0583500 AT3G48040.1 27.485 171 94 4 15 176 8 157 9.85e-17 77.4
Msa0583500 AT1G75840.1 28.302 159 86 4 15 166 6 143 1.18e-16 77.0
Msa0583500 AT2G21880.4 28.877 187 84 5 40 222 9 150 1.64e-16 76.3
Msa0583500 AT2G21880.3 28.877 187 84 5 40 222 9 150 1.64e-16 76.3
Msa0583500 AT2G44690.1 26.946 167 92 4 15 172 18 163 1.84e-16 76.6
Msa0583500 AT1G20090.1 28.302 159 86 4 15 166 5 142 1.85e-16 76.3
Msa0583500 AT5G62880.2 28.743 167 90 4 15 181 8 145 3.54e-16 75.5
Msa0583500 AT4G09720.6 32.973 185 71 6 44 222 3 140 1.13e-15 73.6
Msa0583500 AT4G09720.5 32.973 185 71 6 44 222 3 140 1.13e-15 73.6
Msa0583500 AT4G09720.2 32.973 185 71 6 44 222 3 140 1.13e-15 73.6
Msa0583500 AT4G35020.4 31.852 135 71 3 15 149 6 119 3.68e-15 71.6
Msa0583500 AT4G35020.5 31.852 135 71 3 15 149 6 119 3.68e-15 71.6
Msa0583500 AT2G21880.2 26.415 212 99 6 15 222 9 167 7.92e-15 72.0
Msa0583500 AT5G20020.1 25.676 222 113 7 3 224 2 171 9.46e-15 72.4
Msa0583500 AT5G55190.1 25.455 220 112 7 5 224 4 171 1.03e-14 72.0
Msa0583500 AT5G20010.1 25.455 220 112 7 5 224 4 171 1.25e-14 72.0
Msa0583500 AT4G28950.1 29.091 165 89 6 15 172 6 149 1.30e-14 71.6
Msa0583500 AT5G55080.1 25.118 211 101 6 17 224 15 171 1.72e-12 65.9

Find 73 sgRNAs with CRISPR-Local

Find 269 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTTACTTGTGAAAATTT+TGG 0.139233 4_2:+2158618 Msa0583500:five_prime_UTR
AAAGAAAAGAATGAAATTAT+TGG 0.231257 4_2:-2161565 None:intergenic
AACGTATCAAGCTCCAAATT+TGG 0.259101 4_2:+2159989 Msa0583500:CDS
ACCAGTTTCATCACAACTAT+TGG 0.260192 4_2:+2159844 Msa0583500:CDS
GTCCTGCAGTATCCCAAATT+TGG 0.278971 4_2:-2160002 None:intergenic
GTTGATTTCCTATGCGTTGA+TGG 0.319946 4_2:+2162441 Msa0583500:three_prime_UTR
ATCAGCCAGTGCTTGGCCTT+TGG 0.327266 4_2:-2160858 None:intergenic
TCTAACTACCTCTGTTTGAT+TGG 0.332170 4_2:+2162320 Msa0583500:three_prime_UTR
CAAGACTCTGCAACGGGGTC+TGG 0.345479 4_2:+2162084 Msa0583500:CDS
ACCTCTGTTTGATTGGGATT+AGG 0.371672 4_2:+2162327 Msa0583500:three_prime_UTR
TGGATGAACGCAGAATTCTT+AGG 0.371940 4_2:+2161587 Msa0583500:CDS
ACGTATCAAGCTCCAAATTT+GGG 0.372566 4_2:+2159990 Msa0583500:CDS
ATAAGAACCATTGAACTTGA+TGG 0.385351 4_2:+2159964 Msa0583500:CDS
TTGTGCGTGATAAGTAGTTT+TGG 0.385858 4_2:+2162251 Msa0583500:three_prime_UTR
ATCAACCAAGACTCTGCAAC+GGG 0.391757 4_2:+2162078 Msa0583500:CDS
CCTTACCTGTTGTAATTGTC+CGG 0.397162 4_2:-2160035 None:intergenic
CCAGTTTCATCACAACTATT+GGG 0.400381 4_2:+2159845 Msa0583500:CDS
TGGTGCCATGGGCATATTGC+TGG 0.408906 4_2:+2160207 Msa0583500:CDS
CAAGCACTGGCTGATGAGTA+TGG 0.410586 4_2:+2160866 Msa0583500:CDS
CTATGCGTTGATGGATGCTT+CGG 0.411737 4_2:+2162450 Msa0583500:three_prime_UTR
CTTAATGAGGTAATCGTAAT+CGG 0.415779 4_2:-2158770 None:intergenic
TAATCGGCACGAGCCCTTGC+CGG 0.419982 4_2:-2158754 None:intergenic
CTAACTACCTCTGTTTGATT+GGG 0.420571 4_2:+2162321 Msa0583500:three_prime_UTR
GGTAGGGAACAAAGCAGATA+TGG 0.426102 4_2:+2160444 Msa0583500:CDS
GGATAATGTTAACAAAATAC+TGG 0.430059 4_2:+2160423 Msa0583500:CDS
CTCATTAAGCTTCTCTTGAT+CGG 0.432261 4_2:+2158784 Msa0583500:CDS
CCCAATAGTTGTGATGAAAC+TGG 0.436614 4_2:-2159845 None:intergenic
CAACATTGAGCAACATGCTT+CGG 0.444470 4_2:+2160402 Msa0583500:CDS
CAGATATGGACGAAAGCAAA+AGG 0.446902 4_2:+2160458 Msa0583500:CDS
ACGTTTGCCATCAAGTTCAA+TGG 0.447823 4_2:-2159971 None:intergenic
GATGGCTGCTCCACCGGCAA+GGG 0.449585 4_2:+2158741 Msa0583500:exon
CTCTGCAACGGGGTCTGGAC+AGG 0.453418 4_2:+2162089 Msa0583500:CDS
CTGCAGGACAGGAACGGTTC+CGG 0.459289 4_2:+2160016 Msa0583500:CDS
TAAATAATGCCACATTGAAA+AGG 0.462423 4_2:-2162222 None:intergenic
AGCTTACTATCGTGGTGCCA+TGG 0.468786 4_2:+2160195 Msa0583500:intron
TGATGGCTGCTCCACCGGCA+AGG 0.468923 4_2:+2158740 Msa0583500:exon
CAGACCCCGTTGCAGAGTCT+TGG 0.475391 4_2:-2162083 None:intergenic
AACAGTAATGTTCCTTTCTG+TGG 0.484279 4_2:+2160259 Msa0583500:CDS
TGTATAAACTTGCTTATCAA+TGG 0.489406 4_2:+2162369 Msa0583500:three_prime_UTR
AAATCTGCTTGCTGTGGTTG+AGG 0.490381 4_2:+2162120 Msa0583500:CDS
AACGCATAGTTAGTCGCTTG+TGG 0.495580 4_2:-2162507 None:intergenic
GTGAGAGAAAGAGAGAGAAG+TGG 0.503617 4_2:-2158648 None:intergenic
GCTTACTATCGTGGTGCCAT+GGG 0.506910 4_2:+2160196 Msa0583500:intron
AACGTCCAAAGGCCAAGCAC+TGG 0.513872 4_2:+2160853 Msa0583500:CDS
CGGTTCCGGACAATTACAAC+AGG 0.522865 4_2:+2160030 Msa0583500:CDS
ACGCATAGTTAGTCGCTTGT+GGG 0.527860 4_2:-2162506 None:intergenic
AATGTTAACAAAATACTGGT+AGG 0.531919 4_2:+2160427 Msa0583500:CDS
AGTGCTTGGCCTTTGGACGT+TGG 0.532422 4_2:-2160851 None:intergenic
CATACTCATCAGCCAGTGCT+TGG 0.535062 4_2:-2160865 None:intergenic
CTTCTCTTGATCGGCGATAG+CGG 0.545946 4_2:+2158793 Msa0583500:CDS
TAGGCTGTGCCAACGTCCAA+AGG 0.547979 4_2:+2160842 Msa0583500:intron
AAATTCTTCGAAACTGTAAG+TGG 0.558518 4_2:+2160893 Msa0583500:CDS
AATTTGGGATACTGCAGGAC+AGG 0.565679 4_2:+2160005 Msa0583500:CDS
CTCCAAATTTGGGATACTGC+AGG 0.565715 4_2:+2160000 Msa0583500:CDS
TTCAAACTAAAGCCACAGAA+AGG 0.577022 4_2:-2160271 None:intergenic
TCGGCACGAGCCCTTGCCGG+TGG 0.580971 4_2:-2158751 None:intergenic
CTGTTTGATTGGGATTAGGA+CGG 0.583469 4_2:+2162331 Msa0583500:three_prime_UTR
AGATATGGACGAAAGCAAAA+GGG 0.588273 4_2:+2160459 Msa0583500:CDS
GCTTGCTGACACTGACAACA+AGG 0.590249 4_2:+2161054 Msa0583500:CDS
CGAAGCATCCATCAACGCAT+AGG 0.592132 4_2:-2162449 None:intergenic
TGCAAAGACAAATCTAAATG+TGG 0.596571 4_2:+2160991 Msa0583500:CDS
ATGTTAACAAAATACTGGTA+GGG 0.601571 4_2:+2160428 Msa0583500:CDS
GGGATACTGCAGGACAGGAA+CGG 0.617575 4_2:+2160010 Msa0583500:CDS
CGATCAAGAGAAGCTTAATG+AGG 0.624299 4_2:-2158783 None:intergenic
AAAGACAAATCTAAATGTGG+AGG 0.631704 4_2:+2160994 Msa0583500:CDS
AATCAACCAAGACTCTGCAA+CGG 0.636226 4_2:+2162077 Msa0583500:CDS
TGTGATGAAACTGGTAGTGA+AGG 0.645460 4_2:-2159836 None:intergenic
TGCTTATCAATGGCGTGTAG+TGG 0.653850 4_2:+2162379 Msa0583500:three_prime_UTR
TAGCTAGAGACATAAAACAA+AGG 0.656013 4_2:+2161032 Msa0583500:CDS
TGACACTGACAACAAGGCAG+AGG 0.656933 4_2:+2161060 Msa0583500:CDS
ATAGACCAGCAATATGCCCA+TGG 0.666814 4_2:-2160212 None:intergenic
TCCTAATCCCAATCAAACAG+AGG 0.688148 4_2:-2162328 None:intergenic
TCAACCAAGACTCTGCAACG+GGG 0.752690 4_2:+2162079 Msa0583500:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAAAATAAGTTTATATTAA+TGG - chr4_2:2159759-2159778 None:intergenic 10.0%
!!! TTAATTGATTTTGAAATTAA+AGG - chr4_2:2161324-2161343 None:intergenic 10.0%
!! TTAATTTCAAAATCAATTAA+TGG + chr4_2:2161324-2161343 Msa0583500:intron 10.0%
!!! AGTATCTTTTATATATATAT+GGG - chr4_2:2161940-2161959 None:intergenic 10.0%
!!! TAGTATCTTTTATATATATA+TGG - chr4_2:2161941-2161960 None:intergenic 10.0%
!! AAAAATTGAAAATGCAAATA+TGG - chr4_2:2158673-2158692 None:intergenic 15.0%
!! TGTTATTGTAATGTAAATTA+TGG + chr4_2:2159023-2159042 Msa0583500:intron 15.0%
!! GTATTAAACAAATAAATTGT+GGG - chr4_2:2159250-2159269 None:intergenic 15.0%
!! TGTATTAAACAAATAAATTG+TGG - chr4_2:2159251-2159270 None:intergenic 15.0%
!! ATTTGTTTAATACACAAATT+AGG + chr4_2:2159257-2159276 Msa0583500:intron 15.0%
!!! ATTTGTTATTTATCAATTGT+TGG - chr4_2:2160740-2160759 None:intergenic 15.0%
!! ATTACACAAAATATATATAG+AGG + chr4_2:2161247-2161266 Msa0583500:intron 15.0%
!! ATTGAATAATAGTAATTGTT+AGG + chr4_2:2161352-2161371 Msa0583500:intron 15.0%
!! TTGAATAATAGTAATTGTTA+GGG + chr4_2:2161353-2161372 Msa0583500:intron 15.0%
!! AAAGAAAAGAATGAAATTAT+TGG - chr4_2:2161568-2161587 None:intergenic 15.0%
!!! AATAATTTCATTCTTTTCTT+TGG + chr4_2:2161567-2161586 Msa0583500:CDS 15.0%
!! ATTCAAATTATTAATTCCAT+TGG - chr4_2:2161658-2161677 None:intergenic 15.0%
!!! GTATCTTTTATATATATATG+GGG - chr4_2:2161939-2161958 None:intergenic 15.0%
!!! ATTTTAGAGAGAGAAAATAA+CGG - chr4_2:2158590-2158609 None:intergenic 20.0%
!!! TTCTTTACTTGTGAAAATTT+TGG + chr4_2:2158618-2158637 Msa0583500:five_prime_UTR 20.0%
!! AAAAATGAATCTGTTGAAAA+TGG - chr4_2:2158714-2158733 None:intergenic 20.0%
!! ACCGTATAATAATATAAACT+AGG + chr4_2:2158853-2158872 Msa0583500:intron 20.0%
!! CCCTAGTTTATATTATTATA+CGG - chr4_2:2158857-2158876 None:intergenic 20.0%
!! CCGTATAATAATATAAACTA+GGG + chr4_2:2158854-2158873 Msa0583500:intron 20.0%
!!! GATTTGTTTTAAATTGTGAA+AGG + chr4_2:2158950-2158969 Msa0583500:intron 20.0%
!! CAAACAATGATTCAAATAAT+GGG - chr4_2:2159003-2159022 None:intergenic 20.0%
!! ACAAACAATGATTCAAATAA+TGG - chr4_2:2159004-2159023 None:intergenic 20.0%
!! TTTGTCATTGTCAATAATTT+GGG - chr4_2:2159137-2159156 None:intergenic 20.0%
!! TTAAAATCAATACTGCATTA+AGG - chr4_2:2159943-2159962 None:intergenic 20.0%
!! TAATAAAAAGAAAACCTATG+AGG - chr4_2:2160069-2160088 None:intergenic 20.0%
!!! CTGCAAAAAACAAATATTTT+AGG - chr4_2:2160177-2160196 None:intergenic 20.0%
!! TTCAAAATCAATTAATGGTT+AGG + chr4_2:2161329-2161348 Msa0583500:intron 20.0%
!!! AAATTGATTTTGTCATGATA+AGG - chr4_2:2161434-2161453 None:intergenic 20.0%
!! TGAAATTATTGGAATCTAAA+TGG - chr4_2:2161557-2161576 None:intergenic 20.0%
!! TATACTATGATAAAAAGATC+TGG + chr4_2:2161688-2161707 Msa0583500:intron 20.0%
!!! TAAATAAACTTTAAAGCTGT+TGG + chr4_2:2161844-2161863 Msa0583500:intron 20.0%
!! TGTTGGATTAAAAATAATTG+AGG + chr4_2:2161861-2161880 Msa0583500:intron 20.0%
!! GTTGGATTAAAAATAATTGA+GGG + chr4_2:2161862-2161881 Msa0583500:intron 20.0%
!! GTTTATACAGTGAAAAAAAT+GGG - chr4_2:2162358-2162377 None:intergenic 20.0%
!! AGTTTATACAGTGAAAAAAA+TGG - chr4_2:2162359-2162378 None:intergenic 20.0%
!! TTTTCACAAGTAAAGAAACA+TGG - chr4_2:2158615-2158634 None:intergenic 25.0%
!! TTTACTTGTGAAAATTTTGG+AGG + chr4_2:2158621-2158640 Msa0583500:five_prime_UTR 25.0%
!!! GATTCATTTTTTCGTTTTGA+TGG + chr4_2:2158723-2158742 Msa0583500:exon 25.0%
! ACGGTAAAAAATACATAGAA+GGG - chr4_2:2158838-2158857 None:intergenic 25.0%
! TACGGTAAAAAATACATAGA+AGG - chr4_2:2158839-2158858 None:intergenic 25.0%
!!! TTTTGACCTTGTTTTTGATT+TGG + chr4_2:2158927-2158946 Msa0583500:intron 25.0%
!!! TTTGACCTTGTTTTTGATTT+GGG + chr4_2:2158928-2158947 Msa0583500:intron 25.0%
! ATGTAAATTATGGTATCAAG+AGG + chr4_2:2159033-2159052 Msa0583500:intron 25.0%
! ATTACTGCAATTGTAATGAA+CGG - chr4_2:2159114-2159133 None:intergenic 25.0%
! CTTTGTCATTGTCAATAATT+TGG - chr4_2:2159138-2159157 None:intergenic 25.0%
! AAACTTATAAGCTAAGAGAA+TGG - chr4_2:2159199-2159218 None:intergenic 25.0%
! ATACTTCATTATGTAAACCA+AGG - chr4_2:2159680-2159699 None:intergenic 25.0%
!!! ATTTTGTTATTGTTCTTCAC+AGG + chr4_2:2159772-2159791 Msa0583500:intron 25.0%
!! AACTATTGGGTATGATTTTA+TGG + chr4_2:2159858-2159877 Msa0583500:intron 25.0%
!!! TTTTTTTTCTTCCTTTCCAT+GGG + chr4_2:2160331-2160350 Msa0583500:intron 25.0%
! TTCTAATGATTTCAGATATC+AGG + chr4_2:2160368-2160387 Msa0583500:intron 25.0%
! GGATAATGTTAACAAAATAC+TGG + chr4_2:2160423-2160442 Msa0583500:CDS 25.0%
! AATGTTAACAAAATACTGGT+AGG + chr4_2:2160427-2160446 Msa0583500:CDS 25.0%
! ATGTTAACAAAATACTGGTA+GGG + chr4_2:2160428-2160447 Msa0583500:CDS 25.0%
!! TAAACATTTTTGTTGACGAA+CGG + chr4_2:2160680-2160699 Msa0583500:intron 25.0%
!! GTACTTTTACCTTTCTTTTT+AGG + chr4_2:2160823-2160842 Msa0583500:intron 25.0%
! ATACAGAGTCGATATTATAT+AGG + chr4_2:2161206-2161225 Msa0583500:intron 25.0%
! ACACAAAATATATATAGAGG+AGG + chr4_2:2161250-2161269 Msa0583500:intron 25.0%
! CACAAAATATATATAGAGGA+GGG + chr4_2:2161251-2161270 Msa0583500:intron 25.0%
! TAAGTTTATAGACTTACTCT+TGG - chr4_2:2161288-2161307 None:intergenic 25.0%
! TGAAATTAAAGGTCATGATT+TGG - chr4_2:2161313-2161332 None:intergenic 25.0%
!!! TAACCAAAGAAGTACATTTT+GGG - chr4_2:2161529-2161548 None:intergenic 25.0%
!!! TTAACCAAAGAAGTACATTT+TGG - chr4_2:2161530-2161549 None:intergenic 25.0%
!! CTTTTATATATATATGGGGA+GGG - chr4_2:2161935-2161954 None:intergenic 25.0%
!! TCTTTTATATATATATGGGG+AGG - chr4_2:2161936-2161955 None:intergenic 25.0%
! AATATCGTATCGTGTAAATA+AGG - chr4_2:2161965-2161984 None:intergenic 25.0%
!!! AAAAGGTATCACAAAACATT+AGG - chr4_2:2162036-2162055 None:intergenic 25.0%
! TAAATAATGCCACATTGAAA+AGG - chr4_2:2162225-2162244 None:intergenic 25.0%
!! TGTATAAACTTGCTTATCAA+TGG + chr4_2:2162369-2162388 Msa0583500:three_prime_UTR 25.0%
CTTAATGAGGTAATCGTAAT+CGG - chr4_2:2158773-2158792 None:intergenic 30.0%
GGTAAAAAATACATAGAAGG+GGG - chr4_2:2158836-2158855 None:intergenic 30.0%
CGGTAAAAAATACATAGAAG+GGG - chr4_2:2158837-2158856 None:intergenic 30.0%
CAAATGTTACATAAGAACGA+AGG + chr4_2:2158882-2158901 Msa0583500:intron 30.0%
CAAATCCCAAATCAAAAACA+AGG - chr4_2:2158936-2158955 None:intergenic 30.0%
! TGAAAGGTTTGATCTTTTCA+TGG + chr4_2:2158966-2158985 Msa0583500:intron 30.0%
TAATGAACGGATCACATAAA+AGG - chr4_2:2159101-2159120 None:intergenic 30.0%
GTAATTGAGATCAACTAAAG+GGG - chr4_2:2159396-2159415 None:intergenic 30.0%
GGTAATTGAGATCAACTAAA+GGG - chr4_2:2159397-2159416 None:intergenic 30.0%
AGGTAATTGAGATCAACTAA+AGG - chr4_2:2159398-2159417 None:intergenic 30.0%
ACCTTCTTGTGTAAAATACA+GGG + chr4_2:2159490-2159509 Msa0583500:intron 30.0%
TGTACAATACACTAATTGGT+GGG + chr4_2:2159521-2159540 Msa0583500:intron 30.0%
AGCAACACAGTTATTCAAAT+TGG - chr4_2:2159711-2159730 None:intergenic 30.0%
TGAGATATTAGAGAAATCTC+AGG - chr4_2:2159914-2159933 None:intergenic 30.0%
ATAAGAACCATTGAACTTGA+TGG + chr4_2:2159964-2159983 Msa0583500:CDS 30.0%
!!! GTTTTTTTTCTTCCTTTCCA+TGG + chr4_2:2160330-2160349 Msa0583500:intron 30.0%
! ATCTGAAATCATTAGAAGCT+AGG - chr4_2:2160365-2160384 None:intergenic 30.0%
TGATTTCAGATATCAGGAAT+TGG + chr4_2:2160374-2160393 Msa0583500:intron 30.0%
! GTAAAGGTTGATTTACAAAG+AGG - chr4_2:2160562-2160581 None:intergenic 30.0%
AGTACATTTGACAACTGTAA+AGG - chr4_2:2160578-2160597 None:intergenic 30.0%
GTAAATTTGCATGACGATTA+TGG - chr4_2:2160602-2160621 None:intergenic 30.0%
TAATCGTCATGCAAATTTAC+TGG + chr4_2:2160602-2160621 Msa0583500:intron 30.0%
AAGGTTGAATTGCATTGTTA+AGG + chr4_2:2160625-2160644 Msa0583500:intron 30.0%
! AAACATTTTTGTTGACGAAC+GGG + chr4_2:2160681-2160700 Msa0583500:intron 30.0%
AAATTCTTCGAAACTGTAAG+TGG + chr4_2:2160893-2160912 Msa0583500:CDS 30.0%
! GTGTATTTTACCTGAAAATG+TGG + chr4_2:2160915-2160934 Msa0583500:CDS 30.0%
TGCAAAGACAAATCTAAATG+TGG + chr4_2:2160991-2161010 Msa0583500:CDS 30.0%
AAAGACAAATCTAAATGTGG+AGG + chr4_2:2160994-2161013 Msa0583500:CDS 30.0%
!! TTGTTTTATGTCTCTAGCTA+TGG - chr4_2:2161032-2161051 None:intergenic 30.0%
TAGCTAGAGACATAAAACAA+AGG + chr4_2:2161032-2161051 Msa0583500:CDS 30.0%
TTAGAGACATGAAACTATGA+CGG - chr4_2:2161122-2161141 None:intergenic 30.0%
GAGAAAAGTAGAAAGACATA+TGG + chr4_2:2161165-2161184 Msa0583500:intron 30.0%
TAATAGTAATTGTTAGGGTG+AGG + chr4_2:2161358-2161377 Msa0583500:intron 30.0%
TTAGGTAATCCCAATTTAAG+AGG + chr4_2:2161605-2161624 Msa0583500:intron 30.0%
!!! TGATTTGTTTTTGTCGTTTG+CGG + chr4_2:2161761-2161780 Msa0583500:intron 30.0%
! ATTTTGTTGAGTGTGTTTAG+AGG + chr4_2:2161796-2161815 Msa0583500:intron 30.0%
TAAGTTTAGAGACTTACTCT+TGG + chr4_2:2161898-2161917 Msa0583500:intron 30.0%
CTCATTAAGCTTCTCTTGAT+CGG + chr4_2:2158784-2158803 Msa0583500:CDS 35.0%
TACACAAATTAGGCCTTAAG+CGG + chr4_2:2159267-2159286 Msa0583500:intron 35.0%
TGGATCGAATTCTTACCAAA+TGG - chr4_2:2159322-2159341 None:intergenic 35.0%
ACTAGTAGTCTAGTACGTAT+AGG + chr4_2:2159344-2159363 Msa0583500:intron 35.0%
CACCTTCTTGTGTAAAATAC+AGG + chr4_2:2159489-2159508 Msa0583500:intron 35.0%
! ACCCTGTATTTTACACAAGA+AGG - chr4_2:2159494-2159513 None:intergenic 35.0%
CTTGTGTAAAATACAGGGTA+AGG + chr4_2:2159495-2159514 Msa0583500:intron 35.0%
GCTGTGTACAATACACTAAT+TGG + chr4_2:2159517-2159536 Msa0583500:intron 35.0%
GTGTACAATACACTAATTGG+TGG + chr4_2:2159520-2159539 Msa0583500:intron 35.0%
GTAATTGAGATCACCTAAAG+GGG - chr4_2:2159608-2159627 None:intergenic 35.0%
GGTAATTGAGATCACCTAAA+GGG - chr4_2:2159609-2159628 None:intergenic 35.0%
AGGTAATTGAGATCACCTAA+AGG - chr4_2:2159610-2159629 None:intergenic 35.0%
! GCTTTTGTGTGAAACATACT+TGG + chr4_2:2159728-2159747 Msa0583500:intron 35.0%
TTATTGTTCTTCACAGGTGT+TGG + chr4_2:2159778-2159797 Msa0583500:intron 35.0%
! CTTCTTTTGCGTTTTTCTGA+TGG + chr4_2:2159811-2159830 Msa0583500:CDS 35.0%
ACCAGTTTCATCACAACTAT+TGG + chr4_2:2159844-2159863 Msa0583500:CDS 35.0%
CCAGTTTCATCACAACTATT+GGG + chr4_2:2159845-2159864 Msa0583500:CDS 35.0%
AACGTATCAAGCTCCAAATT+TGG + chr4_2:2159989-2160008 Msa0583500:CDS 35.0%
ACGTATCAAGCTCCAAATTT+GGG + chr4_2:2159990-2160009 Msa0583500:CDS 35.0%
GTAAGGTTTATGATCCTCAT+AGG + chr4_2:2160052-2160071 Msa0583500:intron 35.0%
!!! TTTTTTGCAGCTTACTATCG+TGG + chr4_2:2160187-2160206 Msa0583500:intron 35.0%
AACAGTAATGTTCCTTTCTG+TGG + chr4_2:2160259-2160278 Msa0583500:CDS 35.0%
TTCAAACTAAAGCCACAGAA+AGG - chr4_2:2160274-2160293 None:intergenic 35.0%
AGATATGGACGAAAGCAAAA+GGG + chr4_2:2160459-2160478 Msa0583500:CDS 35.0%
! AAAGGGTATGCAAACTTTGT+TGG + chr4_2:2160476-2160495 Msa0583500:intron 35.0%
! AGGTTGATTTACAAAGAGGA+GGG - chr4_2:2160558-2160577 None:intergenic 35.0%
! AAGGTTGATTTACAAAGAGG+AGG - chr4_2:2160559-2160578 None:intergenic 35.0%
! AACATTTTTGTTGACGAACG+GGG + chr4_2:2160682-2160701 Msa0583500:intron 35.0%
TAGAGACATGAAACTATGAC+GGG - chr4_2:2161121-2161140 None:intergenic 35.0%
GACTCTGTATTTCGAAAACA+CGG - chr4_2:2161196-2161215 None:intergenic 35.0%
! GGTTTAATTGTTTTCTCCAC+TGG + chr4_2:2161379-2161398 Msa0583500:intron 35.0%
GATCCCAAAATGTACTTCTT+TGG + chr4_2:2161523-2161542 Msa0583500:intron 35.0%
AGCATCTCACCTCTTAAATT+GGG - chr4_2:2161617-2161636 None:intergenic 35.0%
AAGCATCTCACCTCTTAAAT+TGG - chr4_2:2161618-2161637 None:intergenic 35.0%
AATTTAAGAGGTGAGATGCT+TGG + chr4_2:2161617-2161636 Msa0583500:intron 35.0%
ATTTAAGAGGTGAGATGCTT+GGG + chr4_2:2161618-2161637 Msa0583500:intron 35.0%
GGGATAATTATTGTAGCCAA+TGG + chr4_2:2161639-2161658 Msa0583500:intron 35.0%
AATAATTGAGGGCTGAGATT+TGG + chr4_2:2161873-2161892 Msa0583500:intron 35.0%
!! TGGTTGATTTTGATGGTTGT+TGG - chr4_2:2162066-2162085 None:intergenic 35.0%
!! AGAGTCTTGGTTGATTTTGA+TGG - chr4_2:2162073-2162092 None:intergenic 35.0%
! TTGAGGACAGTTTTACAAGT+TGG + chr4_2:2162137-2162156 Msa0583500:CDS 35.0%
! TTGTGCGTGATAAGTAGTTT+TGG + chr4_2:2162251-2162270 Msa0583500:three_prime_UTR 35.0%
TCTAACTACCTCTGTTTGAT+TGG + chr4_2:2162320-2162339 Msa0583500:three_prime_UTR 35.0%
CTAACTACCTCTGTTTGATT+GGG + chr4_2:2162321-2162340 Msa0583500:three_prime_UTR 35.0%
CGATCAAGAGAAGCTTAATG+AGG - chr4_2:2158786-2158805 None:intergenic 40.0%
GTTTCGGTCTCTTAACCATT+TGG + chr4_2:2159304-2159323 Msa0583500:intron 40.0%
ACGTACTAGACTACTAGTCA+TGG - chr4_2:2159342-2159361 None:intergenic 40.0%
TTGATCTCAATTACCTCGAG+TGG + chr4_2:2159402-2159421 Msa0583500:intron 40.0%
!! TTACACAAGAAGGTGTTTCC+AGG - chr4_2:2159484-2159503 None:intergenic 40.0%
! TGTGATGAAACTGGTAGTGA+AGG - chr4_2:2159839-2159858 None:intergenic 40.0%
CCCAATAGTTGTGATGAAAC+TGG - chr4_2:2159848-2159867 None:intergenic 40.0%
ACGTTTGCCATCAAGTTCAA+TGG - chr4_2:2159974-2159993 None:intergenic 40.0%
CCTTACCTGTTGTAATTGTC+CGG - chr4_2:2160038-2160057 None:intergenic 40.0%
GAAACACACTGTTTGACTGT+TGG + chr4_2:2160122-2160141 Msa0583500:intron 40.0%
CAACATTGAGCAACATGCTT+CGG + chr4_2:2160402-2160421 Msa0583500:CDS 40.0%
CAGATATGGACGAAAGCAAA+AGG + chr4_2:2160458-2160477 Msa0583500:CDS 40.0%
TTGATTTACAAAGAGGAGGG+GGG - chr4_2:2160555-2160574 None:intergenic 40.0%
! GTTGATTTACAAAGAGGAGG+GGG - chr4_2:2160556-2160575 None:intergenic 40.0%
! GGTTGATTTACAAAGAGGAG+GGG - chr4_2:2160557-2160576 None:intergenic 40.0%
CGTCATGCAAATTTACTGGA+AGG + chr4_2:2160606-2160625 Msa0583500:intron 40.0%
TTAGTTCACTCACAGCAACA+TGG - chr4_2:2160663-2160682 None:intergenic 40.0%
! AAGAGGGTTGAGTTCTGATT+TGG + chr4_2:2160712-2160731 Msa0583500:intron 40.0%
TTGGCACAGCCTAAAAAGAA+AGG - chr4_2:2160835-2160854 None:intergenic 40.0%
! CAGAGAGTTTCCACATTTTC+AGG - chr4_2:2160928-2160947 None:intergenic 40.0%
CTCATTCAACATGAGAGAAG+AGG - chr4_2:2161146-2161165 None:intergenic 40.0%
TGGATGAACGCAGAATTCTT+AGG + chr4_2:2161587-2161606 Msa0583500:CDS 40.0%
TTTAAGAGGTGAGATGCTTG+GGG + chr4_2:2161619-2161638 Msa0583500:intron 40.0%
TGTTGAGTGTGTTTAGAGGA+AGG + chr4_2:2161800-2161819 Msa0583500:intron 40.0%
AACATTAGGCTCGTGAGAAA+GGG - chr4_2:2162022-2162041 None:intergenic 40.0%
AAACATTAGGCTCGTGAGAA+AGG - chr4_2:2162023-2162042 None:intergenic 40.0%
! TGGTTGTTGGCTGCAATAAA+AGG - chr4_2:2162053-2162072 None:intergenic 40.0%
AATCAACCAAGACTCTGCAA+CGG + chr4_2:2162077-2162096 Msa0583500:CDS 40.0%
! TGTGCTCAACCTTTTCAATG+TGG + chr4_2:2162213-2162232 Msa0583500:three_prime_UTR 40.0%
ACCTCTGTTTGATTGGGATT+AGG + chr4_2:2162327-2162346 Msa0583500:three_prime_UTR 40.0%
TCCTAATCCCAATCAAACAG+AGG - chr4_2:2162331-2162350 None:intergenic 40.0%
CTGTTTGATTGGGATTAGGA+CGG + chr4_2:2162331-2162350 Msa0583500:three_prime_UTR 40.0%
! GTTGATTTCCTATGCGTTGA+TGG + chr4_2:2162441-2162460 Msa0583500:three_prime_UTR 40.0%
GTGAGAGAAAGAGAGAGAAG+TGG - chr4_2:2158651-2158670 None:intergenic 45.0%
GGTCCAAGTGGTAGATGTTT+CGG + chr4_2:2159288-2159307 Msa0583500:intron 45.0%
AGACCGAAACATCTACCACT+TGG - chr4_2:2159294-2159313 None:intergenic 45.0%
CCTAAAGGGGCTGACATAAA+GGG - chr4_2:2159595-2159614 None:intergenic 45.0%
ACCTAAAGGGGCTGACATAA+AGG - chr4_2:2159596-2159615 None:intergenic 45.0%
GTGATCTCAATTACCTCGAG+TGG + chr4_2:2159614-2159633 Msa0583500:intron 45.0%
! CTCCAAATTTGGGATACTGC+AGG + chr4_2:2160000-2160019 Msa0583500:CDS 45.0%
GTCCTGCAGTATCCCAAATT+TGG - chr4_2:2160005-2160024 None:intergenic 45.0%
! AATTTGGGATACTGCAGGAC+AGG + chr4_2:2160005-2160024 Msa0583500:CDS 45.0%
CCGGACAATTACAACAGGTA+AGG + chr4_2:2160035-2160054 Msa0583500:intron 45.0%
ACTGTTTGACTGTTGGCATC+TGG + chr4_2:2160129-2160148 Msa0583500:intron 45.0%
ATAGACCAGCAATATGCCCA+TGG - chr4_2:2160215-2160234 None:intergenic 45.0%
AGGTGAATACTCCCATGGAA+AGG - chr4_2:2160345-2160364 None:intergenic 45.0%
AAGCTAGGTGAATACTCCCA+TGG - chr4_2:2160350-2160369 None:intergenic 45.0%
GGTAGGGAACAAAGCAGATA+TGG + chr4_2:2160444-2160463 Msa0583500:CDS 45.0%
TGATTTACAAAGAGGAGGGG+GGG - chr4_2:2160554-2160573 None:intergenic 45.0%
! ATTTTTGTTGACGAACGGGG+AGG + chr4_2:2160685-2160704 Msa0583500:intron 45.0%
ACACACATATACGAGAGAGG+AGG - chr4_2:2160768-2160787 None:intergenic 45.0%
GACACACACATATACGAGAG+AGG - chr4_2:2160771-2160790 None:intergenic 45.0%
! TTGCAGCAACAAGCGTTTTG+CGG - chr4_2:2161409-2161428 None:intergenic 45.0%
!! TGTTTTTGTCGTTTGCGGCA+AGG + chr4_2:2161766-2161785 Msa0583500:intron 45.0%
!! GTTTTTGTCGTTTGCGGCAA+GGG + chr4_2:2161767-2161786 Msa0583500:intron 45.0%
GTGTGTTTAGAGGAAGGAAG+CGG + chr4_2:2161806-2161825 Msa0583500:intron 45.0%
ATCAACCAAGACTCTGCAAC+GGG + chr4_2:2162078-2162097 Msa0583500:CDS 45.0%
! GCACAAAAATCTGCTTGCTG+TGG + chr4_2:2162114-2162133 Msa0583500:CDS 45.0%
!! AAATCTGCTTGCTGTGGTTG+AGG + chr4_2:2162120-2162139 Msa0583500:CDS 45.0%
TGCTTATCAATGGCGTGTAG+TGG + chr4_2:2162379-2162398 Msa0583500:three_prime_UTR 45.0%
! CTATGCGTTGATGGATGCTT+CGG + chr4_2:2162450-2162469 Msa0583500:three_prime_UTR 45.0%
CTTCTCTTGATCGGCGATAG+CGG + chr4_2:2158793-2158812 Msa0583500:CDS 50.0%
TCTACCACTTGGACCGCTTA+AGG - chr4_2:2159283-2159302 None:intergenic 50.0%
CTTTACTGTTGCACCACTCG+AGG - chr4_2:2159418-2159437 None:intergenic 50.0%
TCGCTTGTCACGTGACTGAA+AGG + chr4_2:2159444-2159463 Msa0583500:intron 50.0%
AAGGTCACGAGTTCAAGTCC+TGG + chr4_2:2159463-2159482 Msa0583500:intron 50.0%
CACTAAAGCTCCCGCATACA+CGG - chr4_2:2159562-2159581 None:intergenic 50.0%
! ATGCGGGAGCTTTAGTGCAT+CGG + chr4_2:2159565-2159584 Msa0583500:intron 50.0%
CCCTTTATGTCAGCCCCTTT+AGG + chr4_2:2159592-2159611 Msa0583500:intron 50.0%
CGTTGTACGCTGAGATACCT+TGG + chr4_2:2159660-2159679 Msa0583500:intron 50.0%
CGGTTCCGGACAATTACAAC+AGG + chr4_2:2160030-2160049 Msa0583500:CDS 50.0%
! AGCTTACTATCGTGGTGCCA+TGG + chr4_2:2160195-2160214 Msa0583500:intron 50.0%
!! GCTTACTATCGTGGTGCCAT+GGG + chr4_2:2160196-2160215 Msa0583500:intron 50.0%
ATTTACAAAGAGGAGGGGGG+GGG - chr4_2:2160552-2160571 None:intergenic 50.0%
GATTTACAAAGAGGAGGGGG+GGG - chr4_2:2160553-2160572 None:intergenic 50.0%
CATACTCATCAGCCAGTGCT+TGG - chr4_2:2160868-2160887 None:intergenic 50.0%
!! CAAGCACTGGCTGATGAGTA+TGG + chr4_2:2160866-2160885 Msa0583500:CDS 50.0%
! GCTTGCTGACACTGACAACA+AGG + chr4_2:2161054-2161073 Msa0583500:CDS 50.0%
TGACACTGACAACAAGGCAG+AGG + chr4_2:2161060-2161079 Msa0583500:CDS 50.0%
! AGCGTTTTGCGGTATTCCAG+TGG - chr4_2:2161398-2161417 None:intergenic 50.0%
AGAGGAAGGAAGCGGAAAAG+TGG + chr4_2:2161814-2161833 Msa0583500:intron 50.0%
TCAACCAAGACTCTGCAACG+GGG + chr4_2:2162079-2162098 Msa0583500:CDS 50.0%
! CGAAGCATCCATCAACGCAT+AGG - chr4_2:2162452-2162471 None:intergenic 50.0%
!! CGTTTTGATGGCTGCTCCAC+CGG + chr4_2:2158735-2158754 Msa0583500:exon 55.0%
TAGGCCTTAAGCGGTCCAAG+TGG + chr4_2:2159276-2159295 Msa0583500:intron 55.0%
! TGCGGGAGCTTTAGTGCATC+GGG + chr4_2:2159566-2159585 Msa0583500:intron 55.0%
GCAGACACACTAACCACTCG+AGG - chr4_2:2159630-2159649 None:intergenic 55.0%
GGGATACTGCAGGACAGGAA+CGG + chr4_2:2160010-2160029 Msa0583500:CDS 55.0%
!! TGGTGCCATGGGCATATTGC+TGG + chr4_2:2160207-2160226 Msa0583500:CDS 55.0%
TTTACAAAGAGGAGGGGGGG+GGG - chr4_2:2160551-2160570 None:intergenic 55.0%
TAGGCTGTGCCAACGTCCAA+AGG + chr4_2:2160842-2160861 Msa0583500:intron 55.0%
! AGTGCTTGGCCTTTGGACGT+TGG - chr4_2:2160854-2160873 None:intergenic 55.0%
AACGTCCAAAGGCCAAGCAC+TGG + chr4_2:2160853-2160872 Msa0583500:CDS 55.0%
! ATCAGCCAGTGCTTGGCCTT+TGG - chr4_2:2160861-2160880 None:intergenic 55.0%
TAATCGGCACGAGCCCTTGC+CGG - chr4_2:2158757-2158776 None:intergenic 60.0%
CATACACGGCGTCTGGGAAG+GGG - chr4_2:2159548-2159567 None:intergenic 60.0%
GCATACACGGCGTCTGGGAA+GGG - chr4_2:2159549-2159568 None:intergenic 60.0%
CTTCCCAGACGCCGTGTATG+CGG + chr4_2:2159548-2159567 Msa0583500:intron 60.0%
TTCCCAGACGCCGTGTATGC+GGG + chr4_2:2159549-2159568 Msa0583500:intron 60.0%
CTGCAGGACAGGAACGGTTC+CGG + chr4_2:2160016-2160035 Msa0583500:CDS 60.0%
GGGGGAACGAAAAAGGCAGG+AGG - chr4_2:2160533-2160552 None:intergenic 60.0%
ACGAACGGGGAGGTTCGAAG+AGG + chr4_2:2160695-2160714 Msa0583500:intron 60.0%
CGAACGGGGAGGTTCGAAGA+GGG + chr4_2:2160696-2160715 Msa0583500:intron 60.0%
CAGACCCCGTTGCAGAGTCT+TGG - chr4_2:2162086-2162105 None:intergenic 60.0%
CAAGACTCTGCAACGGGGTC+TGG + chr4_2:2162084-2162103 Msa0583500:CDS 60.0%
TGATGGCTGCTCCACCGGCA+AGG + chr4_2:2158740-2158759 Msa0583500:exon 65.0%
GATGGCTGCTCCACCGGCAA+GGG + chr4_2:2158741-2158760 Msa0583500:exon 65.0%
CGCATACACGGCGTCTGGGA+AGG - chr4_2:2159550-2159569 None:intergenic 65.0%
CTCCCGCATACACGGCGTCT+GGG - chr4_2:2159554-2159573 None:intergenic 65.0%
GGGGGGGGAACGAAAAAGGC+AGG - chr4_2:2160536-2160555 None:intergenic 65.0%
GAGGGGGGGGGGAACGAAAA+AGG - chr4_2:2160540-2160559 None:intergenic 65.0%
CTCTGCAACGGGGTCTGGAC+AGG + chr4_2:2162089-2162108 Msa0583500:CDS 65.0%
GCTCCCGCATACACGGCGTC+TGG - chr4_2:2159555-2159574 None:intergenic 70.0%
TCGGCACGAGCCCTTGCCGG+TGG - chr4_2:2158754-2158773 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 2158582 2162521 2158582 ID=Msa0583500;Name=Msa0583500
chr4_2 mRNA 2158582 2162521 2158582 ID=Msa0583500-mRNA-1;Parent=Msa0583500;Name=Msa0583500-mRNA-1;_AED=0.10;_eAED=0.15;_QI=160|0.57|0.5|0.87|1|1|8|347|288
chr4_2 exon 2158582 2158814 2158582 ID=Msa0583500-mRNA-1:exon:576;Parent=Msa0583500-mRNA-1
chr4_2 exon 2159794 2159866 2159794 ID=Msa0583500-mRNA-1:exon:577;Parent=Msa0583500-mRNA-1
chr4_2 exon 2159951 2160051 2159951 ID=Msa0583500-mRNA-1:exon:578;Parent=Msa0583500-mRNA-1
chr4_2 exon 2160197 2160329 2160197 ID=Msa0583500-mRNA-1:exon:579;Parent=Msa0583500-mRNA-1
chr4_2 exon 2160390 2160480 2160390 ID=Msa0583500-mRNA-1:exon:580;Parent=Msa0583500-mRNA-1
chr4_2 exon 2160845 2161081 2160845 ID=Msa0583500-mRNA-1:exon:581;Parent=Msa0583500-mRNA-1
chr4_2 exon 2161562 2161608 2161562 ID=Msa0583500-mRNA-1:exon:582;Parent=Msa0583500-mRNA-1
chr4_2 exon 2162063 2162521 2162063 ID=Msa0583500-mRNA-1:exon:583;Parent=Msa0583500-mRNA-1
chr4_2 five_prime_UTR 2158582 2158741 2158582 ID=Msa0583500-mRNA-1:five_prime_utr;Parent=Msa0583500-mRNA-1
chr4_2 CDS 2158742 2158814 2158742 ID=Msa0583500-mRNA-1:cds;Parent=Msa0583500-mRNA-1
chr4_2 CDS 2159794 2159866 2159794 ID=Msa0583500-mRNA-1:cds;Parent=Msa0583500-mRNA-1
chr4_2 CDS 2159951 2160051 2159951 ID=Msa0583500-mRNA-1:cds;Parent=Msa0583500-mRNA-1
chr4_2 CDS 2160197 2160329 2160197 ID=Msa0583500-mRNA-1:cds;Parent=Msa0583500-mRNA-1
chr4_2 CDS 2160390 2160480 2160390 ID=Msa0583500-mRNA-1:cds;Parent=Msa0583500-mRNA-1
chr4_2 CDS 2160845 2161081 2160845 ID=Msa0583500-mRNA-1:cds;Parent=Msa0583500-mRNA-1
chr4_2 CDS 2161562 2161608 2161562 ID=Msa0583500-mRNA-1:cds;Parent=Msa0583500-mRNA-1
chr4_2 CDS 2162063 2162174 2162063 ID=Msa0583500-mRNA-1:cds;Parent=Msa0583500-mRNA-1
chr4_2 three_prime_UTR 2162175 2162521 2162175 ID=Msa0583500-mRNA-1:three_prime_utr;Parent=Msa0583500-mRNA-1
Gene Sequence

>Msa0583500

ATGGCTGCTCCACCGGCAAGGGCTCGTGCCGATTACGATTACCTCATTAAGCTTCTCTTGATCGGCGATAGCGGTGTTGGAAAAAGTTGTCTTCTTTTGCGTTTTTCTGATGGTTCCTTCACTACCAGTTTCATCACAACTATTGGTATTGATTTTAAGATAAGAACCATTGAACTTGATGGCAAACGTATCAAGCTCCAAATTTGGGATACTGCAGGACAGGAACGGTTCCGGACAATTACAACAGCTTACTATCGTGGTGCCATGGGCATATTGCTGGTCTATGATGTTACTGATGAAGCATCATTTAACAGTAATGTTCCTTTCTGTGGCTTTAGTTTGAAATCTGCTGATCTGTGCAAAACTTGTAAAACTTCAATGAATTGGATTCGCAACATTGAGCAACATGCTTCGGATAATGTTAACAAAATACTGGTAGGGAACAAAGCAGATATGGACGAAAGCAAAAGGGCTGTGCCAACGTCCAAAGGCCAAGCACTGGCTGATGAGTATGGAATCAAATTCTTCGAAACTGTAAGTGGTGTATTTTACCTGAAAATGTGGAAACTCTCTGAACTTCTATGTTGTTATGCTCATGAAAATGCTCAATTGCAGAGTGCAAAGACAAATCTAAATGTGGAGGAAGTTTTCTTTTCCATAGCTAGAGACATAAAACAAAGGCTTGCTGACACTGACAACAAGGCAGAGATTCCAATAATTTCATTCTTTTCTTTGGATGAACGCAGAATTCTTAGCCAACAACCATCAAAATCAACCAAGACTCTGCAACGGGGTCTGGACAGGCTGCACAAAAATCTGCTTGCTGTGGTTGAGGACAGTTTTACAAGTTGGTTGCAACATTATTGA
Protein sequence

>Msa0583500

MAAPPARARADYDYLIKLLLIGDSGVGKSCLLLRFSDGSFTTSFITTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGILLVYDVTDEASFNSNVPFCGFSLKSADLCKTCKTSMNWIRNIEQHASDNVNKILVGNKADMDESKRAVPTSKGQALADEYGIKFFETVSGVFYLKMWKLSELLCCYAHENAQLQSAKTNLNVEEVFFSIARDIKQRLADTDNKAEIPIISFFSLDERRILSQQPSKSTKTLQRGLDRLHKNLLAVVEDSFTSWLQHY*