AlfalfaGEDB | Msa1089270

M. sativa cultivar zhongmu-4 / Msa1089270

KEGG: K17794
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005744 GO:0006605 GO:0006626 GO:0006810 GO:0006839 GO:0006886 GO:0006996 GO:0007005 GO:0008104 GO:0008150 GO:0009987 GO:0015031 GO:0015833 GO:0016020 GO:0016021 GO:0016043 GO:0017038 GO:0019866 GO:0030150 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031304 GO:0031305 GO:0031966 GO:0031967 GO:0031975 GO:0032592 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0044743 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055085 GO:0065002 GO:0070585 GO:0070727 GO:0071702 GO:0071705 GO:0071806 GO:0071840 GO:0072594 GO:0072655 GO:0098573 GO:0098796 GO:0098798 GO:0098800 GO:1990542
PFAM: PF02466.22

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1089270	XP_003597557.1	99.468	188	1	0	1	188	1	188	1.20e-133	382

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1089270	sp\|Q38820\|TI232_ARATH	67.568	185	57	1	7	188	4	188	2.36e-75	227

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1089270	G7IS28	99.468	188	1	0	1	188	1	188	5.72e-134	382

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0006880	Msa1089270	0.806704	7.226770e-50	-8.615850e-47
Msa0007850	Msa1089270	0.839585	1.450695e-57	-8.615850e-47
Msa0012710	Msa1089270	0.835713	1.429809e-56	-8.615850e-47
Msa0017320	Msa1089270	0.824016	1.014319e-53	-8.615850e-47
Msa0033880	Msa1089270	0.819777	9.719895e-53	-8.615850e-47
Msa0047250	Msa1089270	0.810143	1.334437e-50	-8.615850e-47
Msa0056270	Msa1089270	0.820768	5.760853e-53	-8.615850e-47
Msa0061500	Msa1089270	0.804040	2.614474e-49	-8.615850e-47
Msa0065650	Msa1089270	0.812395	4.333132e-51	-8.615850e-47
Msa0070430	Msa1089270	0.810978	8.808014e-51	-8.615850e-47
Msa0072170	Msa1089270	0.801335	9.454254e-49	-8.615850e-47
Msa0074750	Msa1089270	0.813471	2.517621e-51	-8.615850e-47
Msa0076780	Msa1089270	0.825499	4.534818e-54	-8.615850e-47
Msa0077540	Msa1089270	0.807739	4.363297e-50	-8.615850e-47
Msa0077900	Msa1089270	0.824862	6.411589e-54	-8.615850e-47
Msa0095770	Msa1089270	0.803099	4.097384e-49	-8.615850e-47
Msa0097950	Msa1089270	0.815840	7.523294e-52	-8.615850e-47
Msa0100800	Msa1089270	0.839534	1.496010e-57	-8.615850e-47
Msa0106050	Msa1089270	0.842309	2.797050e-58	-8.615850e-47
Msa0108870	Msa1089270	0.815977	7.010966e-52	-8.615850e-47
Msa0111080	Msa1089270	0.805357	1.388198e-49	-8.615850e-47
Msa0606810	Msa1089270	0.807667	4.520074e-50	-8.615850e-47
Msa0610090	Msa1089270	0.817312	3.519827e-52	-8.615850e-47
Msa0615680	Msa1089270	0.810912	9.102341e-51	-8.615850e-47
Msa0618930	Msa1089270	0.816849	4.472187e-52	-8.615850e-47
Msa0626940	Msa1089270	0.804661	1.941008e-49	-8.615850e-47
Msa0654870	Msa1089270	0.824591	7.431036e-54	-8.615850e-47
Msa0656810	Msa1089270	0.825656	4.162108e-54	-8.615850e-47
Msa0658480	Msa1089270	0.822614	2.155755e-53	-8.615850e-47
Msa0659550	Msa1089270	0.805667	1.194911e-49	-8.615850e-47
Msa0659790	Msa1089270	0.837384	5.366899e-57	-8.615850e-47
Msa0687230	Msa1089270	0.828663	7.930456e-55	-8.615850e-47
Msa0689630	Msa1089270	-0.807346	5.287074e-50	-8.615850e-47
Msa0699780	Msa1089270	0.800274	1.556351e-48	-8.615850e-47
Msa0712560	Msa1089270	0.810151	1.329442e-50	-8.615850e-47
Msa0743530	Msa1089270	0.837142	6.187986e-57	-8.615850e-47
Msa0744100	Msa1089270	0.829405	5.242602e-55	-8.615850e-47
Msa0744180	Msa1089270	0.828190	1.031697e-54	-8.615850e-47
Msa0746170	Msa1089270	0.808789	2.605739e-50	-8.615850e-47
Msa0746210	Msa1089270	0.804109	2.529159e-49	-8.615850e-47
Msa0748660	Msa1089270	0.802211	6.247325e-49	-8.615850e-47
Msa0766670	Msa1089270	0.823866	1.099739e-53	-8.615850e-47
Msa0788120	Msa1089270	0.811579	6.524252e-51	-8.615850e-47
Msa0789560	Msa1089270	0.807998	3.842596e-50	-8.615850e-47
Msa0818770	Msa1089270	0.826885	2.121860e-54	-8.615850e-47
Msa0819540	Msa1089270	0.802137	6.471133e-49	-8.615850e-47
Msa0822170	Msa1089270	0.845036	5.214992e-59	-8.615850e-47
Msa0822180	Msa1089270	0.801282	9.692964e-49	-8.615850e-47
Msa0825270	Msa1089270	0.839471	1.553682e-57	-8.615850e-47
Msa0826510	Msa1089270	0.808889	2.481144e-50	-8.615850e-47
Msa0839450	Msa1089270	0.806222	9.132729e-50	-8.615850e-47
Msa0125790	Msa1089270	0.800287	1.547192e-48	-8.615850e-47
Msa0126840	Msa1089270	0.805468	1.315648e-49	-8.615850e-47
Msa0126880	Msa1089270	0.851319	9.592230e-61	-8.615850e-47
Msa0127040	Msa1089270	0.816129	6.482545e-52	-8.615850e-47
Msa0127850	Msa1089270	0.816550	5.219642e-52	-8.615850e-47
Msa0142110	Msa1089270	0.830965	2.182178e-55	-8.615850e-47
Msa0142410	Msa1089270	0.822250	2.618807e-53	-8.615850e-47
Msa0142500	Msa1089270	0.833018	6.783779e-56	-8.615850e-47
Msa0146080	Msa1089270	0.824380	8.328836e-54	-8.615850e-47
Msa0152560	Msa1089270	0.815816	7.615306e-52	-8.615850e-47
Msa0166750	Msa1089270	0.819245	1.284995e-52	-8.615850e-47
Msa0171980	Msa1089270	0.838346	3.036832e-57	-8.615850e-47
Msa0174000	Msa1089270	0.840880	6.659722e-58	-8.615850e-47
Msa0193600	Msa1089270	0.828658	7.952725e-55	-8.615850e-47
Msa0197530	Msa1089270	0.801383	9.240457e-49	-8.615850e-47
Msa0198520	Msa1089270	0.808804	2.587443e-50	-8.615850e-47
Msa0198530	Msa1089270	0.802692	4.971975e-49	-8.615850e-47
Msa0202930	Msa1089270	0.816661	4.929540e-52	-8.615850e-47
Msa0208000	Msa1089270	0.817485	3.217842e-52	-8.615850e-47
Msa0229360	Msa1089270	0.832019	1.200383e-55	-8.615850e-47
Msa0238030	Msa1089270	0.815425	9.307369e-52	-8.615850e-47
Msa0382400	Msa1089270	0.811646	6.309835e-51	-8.615850e-47
Msa0384670	Msa1089270	0.842245	2.908186e-58	-8.615850e-47
Msa0384880	Msa1089270	0.804210	2.409951e-49	-8.615850e-47
Msa0403770	Msa1089270	0.805681	1.187081e-49	-8.615850e-47
Msa0434570	Msa1089270	0.827521	1.494217e-54	-8.615850e-47
Msa0434770	Msa1089270	0.838719	2.433282e-57	-8.615850e-47
Msa0436200	Msa1089270	0.834059	3.730464e-56	-8.615850e-47
Msa0439890	Msa1089270	0.803517	3.356728e-49	-8.615850e-47
Msa0442310	Msa1089270	0.807005	6.244544e-50	-8.615850e-47
Msa0472720	Msa1089270	0.816291	5.964925e-52	-8.615850e-47
Msa0472770	Msa1089270	0.806404	8.364284e-50	-8.615850e-47
Msa0478580	Msa1089270	0.847196	1.347504e-59	-8.615850e-47
Msa0479330	Msa1089270	0.832122	1.131861e-55	-8.615850e-47
Msa1001350	Msa1089270	0.803389	3.569070e-49	-8.615850e-47
Msa1025940	Msa1089270	0.803688	3.093978e-49	-8.615850e-47
Msa1030930	Msa1089270	0.811826	5.763902e-51	-8.615850e-47
Msa1034720	Msa1089270	0.813487	2.496839e-51	-8.615850e-47
Msa1036740	Msa1089270	0.831040	2.090946e-55	-8.615850e-47
Msa1036910	Msa1089270	0.807333	5.319678e-50	-8.615850e-47
Msa1042660	Msa1089270	0.803743	3.014004e-49	-8.615850e-47
Msa1070050	Msa1089270	0.810786	9.695653e-51	-8.615850e-47
Msa1070390	Msa1089270	0.850309	1.846245e-60	-8.615850e-47
Msa1072600	Msa1089270	0.800929	1.144376e-48	-8.615850e-47
Msa1089270	Msa1093100	0.829010	6.538547e-55	-8.615850e-47
Msa1089270	Msa1095790	0.808588	2.877330e-50	-8.615850e-47
Msa1089270	Msa1113880	0.801236	9.902305e-49	-8.615850e-47
Msa1089270	Msa1125790	-0.814173	1.762610e-51	-8.615850e-47
Msa1089270	Msa1127430	0.811292	7.531767e-51	-8.615850e-47
Msa1089270	Msa1127540	0.822164	2.742828e-53	-8.615850e-47
Msa1089270	Msa1136790	0.808182	3.511483e-50	-8.615850e-47
Msa1089270	Msa1158230	0.802775	4.779617e-49	-8.615850e-47
Msa1089270	Msa1162080	0.821147	4.712258e-53	-8.615850e-47
Msa1089270	Msa1163870	0.810799	9.629445e-51	-8.615850e-47
Msa1089270	Msa1164020	0.813646	2.303997e-51	-8.615850e-47
Msa1089270	Msa1165780	0.805954	1.040054e-49	-8.615850e-47
Msa1089270	Msa1166870	0.809336	1.990342e-50	-8.615850e-47
Msa1089270	Msa1173900	0.805258	1.455707e-49	-8.615850e-47
Msa1089270	Msa1176980	0.814560	1.447735e-51	-8.615850e-47
Msa1089270	Msa1188560	0.832691	8.180341e-56	-8.615850e-47
Msa1089270	Msa1197070	0.838574	2.652724e-57	-8.615850e-47
Msa1089270	Msa1201640	0.807500	4.904859e-50	-8.615850e-47
Msa1089270	Msa1201830	0.801521	8.658292e-49	-8.615850e-47
Msa1089270	Msa1205480	0.828357	9.401680e-55	-8.615850e-47
Msa1089270	Msa1205850	0.824893	6.307168e-54	-8.615850e-47
Msa1089270	Msa1206650	0.826451	2.694066e-54	-8.615850e-47
Msa1089270	Msa1207730	0.847858	8.863175e-60	-8.615850e-47
Msa1089270	Msa1211350	0.807410	5.123842e-50	-8.615850e-47
Msa1089270	Msa1226610	0.815788	7.724332e-52	-8.615850e-47
Msa1089270	Msa1242640	0.807100	5.959831e-50	-8.615850e-47
Msa1089270	Msa1250110	0.827065	1.921440e-54	-8.615850e-47
Msa1089270	Msa1250730	0.824924	6.200854e-54	-8.615850e-47
Msa1089270	Msa1257070	0.807507	4.887679e-50	-8.615850e-47
Msa1089270	Msa1270620	0.802648	5.077444e-49	-8.615850e-47
Msa1089270	Msa1296300	0.813699	2.243150e-51	-8.615850e-47
Msa1089270	Msa1299700	0.820322	7.291978e-53	-8.615850e-47
Msa1089270	Msa1304810	0.811812	5.805612e-51	-8.615850e-47
Msa1089270	Msa1307180	0.812859	3.430148e-51	-8.615850e-47
Msa1089270	Msa1316360	0.803862	2.846399e-49	-8.615850e-47
Msa1089270	Msa1318620	0.823675	1.219233e-53	-8.615850e-47
Msa1089270	Msa1329300	0.804805	1.811385e-49	-8.615850e-47
Msa1089270	Msa1329450	0.803361	3.617017e-49	-8.615850e-47
Msa1089270	Msa1329800	0.814218	1.723545e-51	-8.615850e-47
Msa1089270	Msa1332220	0.802194	6.297075e-49	-8.615850e-47
Msa1089270	Msa1336040	0.807405	5.136463e-50	-8.615850e-47
Msa1089270	Msa1344310	0.812558	3.991974e-51	-8.615850e-47
Msa1089270	Msa1348650	-0.804055	2.595621e-49	-8.615850e-47
Msa1089270	Msa1349570	0.804972	1.671254e-49	-8.615850e-47
Msa1089270	Msa1352890	0.806304	8.777177e-50	-8.615850e-47
Msa1089270	Msa1355700	0.812634	3.840329e-51	-8.615850e-47
Msa1089270	Msa1361250	0.820669	6.070883e-53	-8.615850e-47
Msa1089270	Msa1364060	0.800090	1.696336e-48	-8.615850e-47
Msa1089270	Msa1364160	0.995077	8.401188e-213	-8.615850e-47
Msa1089270	Msa1381480	0.807194	5.695191e-50	-8.615850e-47
Msa1089270	Msa1382020	0.806213	9.176414e-50	-8.615850e-47
Msa1089270	Msa1385810	0.814090	1.838672e-51	-8.615850e-47
Msa1089270	Msa1391480	0.818673	1.733366e-52	-8.615850e-47
Msa1089270	Msa1398170	0.803838	2.879166e-49	-8.615850e-47
Msa1089270	Msa1398790	0.815151	1.070866e-51	-8.615850e-47
Msa1089270	Msa1409260	0.826623	2.450568e-54	-8.615850e-47
Msa1089270	Msa1411950	0.800780	1.227200e-48	-8.615850e-47
Msa1089270	Msa1416260	0.830738	2.480147e-55	-8.615850e-47
Msa1089270	Msa1421900	0.829510	4.943336e-55	-8.615850e-47
Msa1089270	Msa1422810	0.805048	1.611069e-49	-8.615850e-47
Msa1089270	Msa1433600	0.817531	3.142057e-52	-8.615850e-47
Msa1089270	Msa1458810	0.803222	3.863389e-49	-8.615850e-47
Msa1089270	Msa1459960	0.846261	2.426926e-59	-8.615850e-47
Msa1089270	Msa1462470	0.845865	3.109564e-59	-8.615850e-47
Msa1089270	Msa1463600	0.836896	7.151306e-57	-8.615850e-47
Msa0239060	Msa1089270	0.803550	3.304834e-49	-8.615850e-47
Msa0244970	Msa1089270	0.802962	4.374113e-49	-8.615850e-47
Msa0244980	Msa1089270	0.803807	2.922850e-49	-8.615850e-47
Msa0247060	Msa1089270	0.829053	6.384184e-55	-8.615850e-47
Msa0262480	Msa1089270	0.853937	1.717216e-61	-8.615850e-47
Msa0265160	Msa1089270	0.830768	2.438207e-55	-8.615850e-47
Msa0273650	Msa1089270	0.829234	5.769370e-55	-8.615850e-47
Msa0274100	Msa1089270	0.814939	1.193158e-51	-8.615850e-47
Msa0300350	Msa1089270	0.854083	1.558514e-61	-8.615850e-47
Msa0313930	Msa1089270	0.809802	1.580462e-50	-8.615850e-47
Msa0321690	Msa1089270	0.804085	2.558316e-49	-8.615850e-47
Msa0322900	Msa1089270	0.814813	1.272399e-51	-8.615850e-47
Msa0324240	Msa1089270	0.811352	7.307622e-51	-8.615850e-47
Msa0358120	Msa1089270	0.809061	2.279724e-50	-8.615850e-47
Msa0363060	Msa1089270	0.818760	1.656395e-52	-8.615850e-47
Msa0511150	Msa1089270	0.820336	7.238841e-53	-8.615850e-47
Msa0514610	Msa1089270	0.807530	4.832948e-50	-8.615850e-47
Msa0524590	Msa1089270	0.822537	2.246006e-53	-8.615850e-47
Msa0547990	Msa1089270	0.805843	1.097373e-49	-8.615850e-47
Msa0550540	Msa1089270	0.825551	4.406463e-54	-8.615850e-47
Msa0550570	Msa1089270	0.847778	9.324392e-60	-8.615850e-47
Msa0561430	Msa1089270	0.825782	3.885861e-54	-8.615850e-47
Msa0569780	Msa1089270	0.810660	1.032087e-50	-8.615850e-47
Msa0582280	Msa1089270	0.824328	8.564984e-54	-8.615850e-47
Msa0583500	Msa1089270	0.816032	6.814063e-52	-8.615850e-47
Msa0593700	Msa1089270	0.804362	2.240119e-49	-8.615850e-47
Msa0602370	Msa1089270	0.806006	1.014368e-49	-8.615850e-47
Msa0858440	Msa1089270	0.803146	4.007438e-49	-8.615850e-47
Msa0859180	Msa1089270	0.817650	2.953425e-52	-8.615850e-47
Msa0864420	Msa1089270	0.812085	5.061840e-51	-8.615850e-47
Msa0865620	Msa1089270	0.801431	9.031606e-49	-8.615850e-47
Msa0870650	Msa1089270	0.820778	5.729423e-53	-8.615850e-47
Msa0879850	Msa1089270	0.805021	1.632192e-49	-8.615850e-47
Msa0882730	Msa1089270	0.838276	3.166640e-57	-8.615850e-47
Msa0901450	Msa1089270	0.801526	8.636081e-49	-8.615850e-47
Msa0921870	Msa1089270	0.808582	2.885893e-50	-8.615850e-47
Msa0925770	Msa1089270	0.801048	1.082242e-48	-8.615850e-47
Msa0947800	Msa1089270	0.838044	3.632992e-57	-8.615850e-47
Msa0963020	Msa1089270	0.804822	1.796570e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1089270	MtrunA17_Chr2g0329891	99.468	188	1	0	1	188	1	188	1.10e-137	382
Msa1089270	MtrunA17_Chr8g0343071	75.419	179	44	0	10	188	4	182	1.96e-85	249
Msa1089270	MtrunA17_Chr1g0190881	61.749	183	69	1	7	188	3	185	5.31e-76	226

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1089270	AT1G72750.1	67.568	185	57	1	7	188	4	188	2.40e-76	227
Msa1089270	AT1G17530.1	62.366	186	64	2	5	188	6	187	1.67e-74	222
Msa1089270	AT1G17530.2	62.295	183	63	2	4	184	5	183	3.50e-73	219
Msa1089270	AT3G04800.1	52.459	183	86	1	7	188	6	188	7.84e-51	162

Find 72 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGGCAGCAGGGCTTGGTACC+GGG	0.325322	7_3:-2617398	Msa1089270:CDS
TTGGGAGCTGGTAGAGGTTT+CGG	0.328905	7_3:+2617730	None:intergenic
TAGGGATTGTAGAGACGTTT+TGG	0.331643	7_3:+2617774	None:intergenic
GTAGCCGCATCCGGTGTAAA+AGG	0.338122	7_3:+2617641	None:intergenic
TTGGTACCGGGGCGTTGTAT+CGG	0.348837	7_3:-2617386	Msa1089270:CDS
ACCTTTCTCGAACGATTTCA+CGG	0.355472	7_3:+2617575	None:intergenic
GTGGCAGCAGGGCTTGGTAC+CGG	0.372617	7_3:-2617399	Msa1089270:CDS
AAGAGATGTTCAGGTGAGGT+TGG	0.384154	7_3:+2617711	None:intergenic
TCCTCGATGAAGAGATGTTC+AGG	0.386072	7_3:+2617702	None:intergenic
GGCTACCTCGGCGGCGCCAT+TGG	0.387745	7_3:-2617624	Msa1089270:CDS
TGGTACCGGGGCGTTGTATC+GGG	0.396607	7_3:-2617385	Msa1089270:CDS
GTGAGATCAGCTGCTGTTGC+CGG	0.398096	7_3:-2617351	Msa1089270:CDS
CGCATCCGGTGTAAAAGGTT+AGG	0.399415	7_3:+2617646	None:intergenic
GCCGCCGAGGTAGCCGCATC+CGG	0.401744	7_3:+2617632	None:intergenic
GGGAACCGCCTTGGTGTGAT+CGG	0.402790	7_3:-2617489	Msa1089270:CDS
TCGGAGTTTGATTCGTGATC+GGG	0.411466	7_3:+2617807	None:intergenic
CACAACACCACCAACAGCAC+CGG	0.411667	7_3:+2617332	None:intergenic
GGCGGCGCCATTGGTGGCGC+CGG	0.412036	7_3:-2617615	Msa1089270:CDS
AACAGTGTGGCAGCAGGGCT+TGG	0.418069	7_3:-2617405	Msa1089270:CDS
GCTGTTGGTGGTGTTGTGGC+TGG	0.423812	7_3:-2617327	Msa1089270:CDS
GGAACCGCCTTGGTGTGATC+GGG	0.429194	7_3:-2617488	Msa1089270:CDS
TACCTCGGCGGCGCCATTGG+TGG	0.432819	7_3:-2617621	Msa1089270:CDS
CGTTGTATCGGGCAGCGAGA+GGG	0.437956	7_3:-2617374	Msa1089270:CDS
GTGAGGTTGGGAGCTGGTAG+AGG	0.443193	7_3:+2617724	None:intergenic
GGATTGGTACTTCGAGGTCT+TGG	0.447073	7_3:+2617751	None:intergenic
CTCAACGCAAACATCGTTCC+TGG	0.447403	7_3:-2617677	Msa1089270:CDS
GTGGCTGGAGTGGCTATCGC+GGG	0.448335	7_3:-2617312	Msa1089270:CDS
AGTGGCTATCGCGGGGAAGC+AGG	0.450298	7_3:-2617304	Msa1089270:CDS
AAAGGTTAGGGTTTCTCCCC+AGG	0.463183	7_3:+2617659	None:intergenic
GCCATTGGTGGCGCCGGCGT+AGG	0.463542	7_3:-2617609	Msa1089270:CDS
GCTGCTGTTGCCGGTGCTGT+TGG	0.465020	7_3:-2617342	Msa1089270:CDS
GCATCCGGTGTAAAAGGTTA+GGG	0.487745	7_3:+2617647	None:intergenic
AACAAGATTCTGAATTCGTC+AGG	0.489468	7_3:-2617531	Msa1089270:CDS
AGAGATGTTCAGGTGAGGTT+GGG	0.491614	7_3:+2617712	None:intergenic
ATCGGAGTTTGATTCGTGAT+CGG	0.492854	7_3:+2617806	None:intergenic
TGGTACTTCGAGGTCTTGGT+AGG	0.496614	7_3:+2617755	None:intergenic
GCCGTGAAATCGTTCGAGAA+AGG	0.498953	7_3:-2617576	Msa1089270:CDS
CGCCACCAATGGCGCCGCCG+AGG	0.504816	7_3:+2617619	None:intergenic
GCTGTTGCCGGTGCTGTTGG+TGG	0.505954	7_3:-2617339	Msa1089270:CDS
GGTTTCGGATTGGTACTTCG+AGG	0.508805	7_3:+2617745	None:intergenic
GTTGTATCGGGCAGCGAGAG+GGG	0.520290	7_3:-2617373	Msa1089270:CDS
ACCTACGCCGGCGCCACCAA+TGG	0.521262	7_3:+2617608	None:intergenic
GCGTTGTATCGGGCAGCGAG+AGG	0.529365	7_3:-2617375	Msa1089270:CDS
TCAACGCAAACATCGTTCCT+GGG	0.532503	7_3:-2617676	Msa1089270:CDS
GGCAGCAGGGCTTGGTACCG+GGG	0.541292	7_3:-2617397	Msa1089270:CDS
TGGAATTGAAAGCGGGATCA+CGG	0.541386	7_3:-2617454	Msa1089270:CDS
CGGTGCTGTTGGTGGTGTTG+TGG	0.552191	7_3:-2617331	Msa1089270:CDS
GATCACGGCGGCGAGAGATA+CGG	0.553451	7_3:-2617439	Msa1089270:CDS
GTGATCGGGTTGTTATTCGC+TGG	0.562580	7_3:-2617474	Msa1089270:CDS
GTTCAGGTGAGGTTGGGAGC+TGG	0.563872	7_3:+2617718	None:intergenic
TGTGGCTGGAGTGGCTATCG+CGG	0.570123	7_3:-2617313	Msa1089270:CDS
TCTGAATTCGTCAGGACACA+CGG	0.572872	7_3:-2617523	Msa1089270:CDS
TGGTGGTGTTGTGGCTGGAG+TGG	0.577673	7_3:-2617322	Msa1089270:CDS
GACACACGGGTCGGACGTGG+GGG	0.579942	7_3:-2617509	Msa1089270:CDS
CGGAGTTTGATTCGTGATCG+GGG	0.580921	7_3:+2617808	None:intergenic
GGTACTTCGAGGTCTTGGTA+GGG	0.582086	7_3:+2617756	None:intergenic
TATTCGCTGGAATTGAAAGC+GGG	0.588545	7_3:-2617461	Msa1089270:CDS
AGCTGGTAGAGGTTTCGGAT+TGG	0.594257	7_3:+2617735	None:intergenic
ACCGGATGCGGCTACCTCGG+CGG	0.600899	7_3:-2617633	Msa1089270:CDS
CTGAATTCGTCAGGACACAC+GGG	0.613772	7_3:-2617522	Msa1089270:CDS
CGCTGCCCGATACAACGCCC+CGG	0.635857	7_3:+2617380	None:intergenic
TTATTCGCTGGAATTGAAAG+CGG	0.641254	7_3:-2617462	Msa1089270:CDS
GGAGTTTGATTCGTGATCGG+GGG	0.642744	7_3:+2617809	None:intergenic
TGGCTGGAGTGGCTATCGCG+GGG	0.652115	7_3:-2617311	Msa1089270:CDS
ATTCGTCAGGACACACGGGT+CGG	0.661517	7_3:-2617518	Msa1089270:CDS
CAACGCAAACATCGTTCCTG+GGG	0.671475	7_3:-2617675	Msa1089270:CDS
GGCTTCGACGAAACCTACGC+CGG	0.685279	7_3:+2617596	None:intergenic
GATGAAGAGATGTTCAGGTG+AGG	0.693036	7_3:+2617707	None:intergenic
ACCTGAACATCTCTTCATCG+AGG	0.710068	7_3:-2617703	Msa1089270:CDS
ATAACAACCCGATCACACCA+AGG	0.726478	7_3:+2617481	None:intergenic
AATTGAAAGCGGGATCACGG+CGG	0.736385	7_3:-2617451	Msa1089270:CDS
ACAACCCGATCACACCAAGG+CGG	0.775839	7_3:+2617484	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AACAAGATTCTGAATTCGTC+AGG	-	chr7_3:2617569-2617588	Msa1089270:CDS	35.0%
	TTATTCGCTGGAATTGAAAG+CGG	-	chr7_3:2617638-2617657	Msa1089270:CDS	35.0%
!	ATCGGAGTTTGATTCGTGAT+CGG	+	chr7_3:2617297-2617316	None:intergenic	40.0%
!!	GATTGTAGAGACGTTTTGGA+GGG	+	chr7_3:2617325-2617344	None:intergenic	40.0%
!	TAGGGATTGTAGAGACGTTT+TGG	+	chr7_3:2617329-2617348	None:intergenic	40.0%
!	GAAACCCTAACCTTTTACAC+CGG	-	chr7_3:2617449-2617468	Msa1089270:CDS	40.0%
	ACCTTTCTCGAACGATTTCA+CGG	+	chr7_3:2617528-2617547	None:intergenic	40.0%
	TATTCGCTGGAATTGAAAGC+GGG	-	chr7_3:2617639-2617658	Msa1089270:CDS	40.0%
!	TCGGAGTTTGATTCGTGATC+GGG	+	chr7_3:2617296-2617315	None:intergenic	45.0%
!!!	ACGTTTTGGAGGGTTGGAAT+CGG	+	chr7_3:2617315-2617334	None:intergenic	45.0%
!	GGATTGTAGAGACGTTTTGG+AGG	+	chr7_3:2617326-2617345	None:intergenic	45.0%
	AGAGATGTTCAGGTGAGGTT+GGG	+	chr7_3:2617391-2617410	None:intergenic	45.0%
	AAGAGATGTTCAGGTGAGGT+TGG	+	chr7_3:2617392-2617411	None:intergenic	45.0%
	GATGAAGAGATGTTCAGGTG+AGG	+	chr7_3:2617396-2617415	None:intergenic	45.0%
	ACCTGAACATCTCTTCATCG+AGG	-	chr7_3:2617397-2617416	Msa1089270:CDS	45.0%
	TCCTCGATGAAGAGATGTTC+AGG	+	chr7_3:2617401-2617420	None:intergenic	45.0%
	TCAACGCAAACATCGTTCCT+GGG	-	chr7_3:2617424-2617443	Msa1089270:CDS	45.0%
	GCATCCGGTGTAAAAGGTTA+GGG	+	chr7_3:2617456-2617475	None:intergenic	45.0%
!	CTAACCTTTTACACCGGATG+CGG	-	chr7_3:2617455-2617474	Msa1089270:CDS	45.0%
	TCTGAATTCGTCAGGACACA+CGG	-	chr7_3:2617577-2617596	Msa1089270:CDS	45.0%
	ATAACAACCCGATCACACCA+AGG	+	chr7_3:2617622-2617641	None:intergenic	45.0%
	TGGAATTGAAAGCGGGATCA+CGG	-	chr7_3:2617646-2617665	Msa1089270:CDS	45.0%
!	GGACGATGTTTTCAACAGTG+TGG	-	chr7_3:2617682-2617701	Msa1089270:CDS	45.0%
!	TTTTCAACAGTGTGGCAGCA+GGG	-	chr7_3:2617690-2617709	Msa1089270:CDS	45.0%
!	GGAGTTTGATTCGTGATCGG+GGG	+	chr7_3:2617294-2617313	None:intergenic	50.0%
!	CGGAGTTTGATTCGTGATCG+GGG	+	chr7_3:2617295-2617314	None:intergenic	50.0%
!!!	GTAGAGACGTTTTGGAGGGT+TGG	+	chr7_3:2617321-2617340	None:intergenic	50.0%
	GGTACTTCGAGGTCTTGGTA+GGG	+	chr7_3:2617347-2617366	None:intergenic	50.0%
	TGGTACTTCGAGGTCTTGGT+AGG	+	chr7_3:2617348-2617367	None:intergenic	50.0%
!	GGATTGGTACTTCGAGGTCT+TGG	+	chr7_3:2617352-2617371	None:intergenic	50.0%
!!	GGTTTCGGATTGGTACTTCG+AGG	+	chr7_3:2617358-2617377	None:intergenic	50.0%
	AGCTGGTAGAGGTTTCGGAT+TGG	+	chr7_3:2617368-2617387	None:intergenic	50.0%
	TTGGGAGCTGGTAGAGGTTT+CGG	+	chr7_3:2617373-2617392	None:intergenic	50.0%
	CTCAACGCAAACATCGTTCC+TGG	-	chr7_3:2617423-2617442	Msa1089270:CDS	50.0%
	CAACGCAAACATCGTTCCTG+GGG	-	chr7_3:2617425-2617444	Msa1089270:CDS	50.0%
	AAAGGTTAGGGTTTCTCCCC+AGG	+	chr7_3:2617444-2617463	None:intergenic	50.0%
	CGCATCCGGTGTAAAAGGTT+AGG	+	chr7_3:2617457-2617476	None:intergenic	50.0%
	GCCGTGAAATCGTTCGAGAA+AGG	-	chr7_3:2617524-2617543	Msa1089270:CDS	50.0%
	CTGAATTCGTCAGGACACAC+GGG	-	chr7_3:2617578-2617597	Msa1089270:CDS	50.0%
!	GTGATCGGGTTGTTATTCGC+TGG	-	chr7_3:2617626-2617645	Msa1089270:CDS	50.0%
	AATTGAAAGCGGGATCACGG+CGG	-	chr7_3:2617649-2617668	Msa1089270:CDS	50.0%
!	GTTTTCAACAGTGTGGCAGC+AGG	-	chr7_3:2617689-2617708	Msa1089270:CDS	50.0%
	GTAGCCGCATCCGGTGTAAA+AGG	+	chr7_3:2617462-2617481	None:intergenic	55.0%
	ATTCGTCAGGACACACGGGT+CGG	-	chr7_3:2617582-2617601	Msa1089270:CDS	55.0%
	ACAACCCGATCACACCAAGG+CGG	+	chr7_3:2617619-2617638	None:intergenic	55.0%
!!	TTGGTACCGGGGCGTTGTAT+CGG	-	chr7_3:2617714-2617733	Msa1089270:CDS	55.0%
	GTGAGATCAGCTGCTGTTGC+CGG	-	chr7_3:2617749-2617768	Msa1089270:CDS	55.0%
	CACAACACCACCAACAGCAC+CGG	+	chr7_3:2617771-2617790	None:intergenic	55.0%
	GTGAGGTTGGGAGCTGGTAG+AGG	+	chr7_3:2617379-2617398	None:intergenic	60.0%
	GTTCAGGTGAGGTTGGGAGC+TGG	+	chr7_3:2617385-2617404	None:intergenic	60.0%
	TACACCGGATGCGGCTACCT+CGG	-	chr7_3:2617464-2617483	Msa1089270:CDS	60.0%
	GGCTTCGACGAAACCTACGC+CGG	+	chr7_3:2617507-2617526	None:intergenic	60.0%
!	GGGAACCGCCTTGGTGTGAT+CGG	-	chr7_3:2617611-2617630	Msa1089270:CDS	60.0%
!	GGAACCGCCTTGGTGTGATC+GGG	-	chr7_3:2617612-2617631	Msa1089270:CDS	60.0%
	GATCACGGCGGCGAGAGATA+CGG	-	chr7_3:2617661-2617680	Msa1089270:CDS	60.0%
	AACAGTGTGGCAGCAGGGCT+TGG	-	chr7_3:2617695-2617714	Msa1089270:CDS	60.0%
!!	TGGTACCGGGGCGTTGTATC+GGG	-	chr7_3:2617715-2617734	Msa1089270:CDS	60.0%
	CGTTGTATCGGGCAGCGAGA+GGG	-	chr7_3:2617726-2617745	Msa1089270:CDS	60.0%
	GTTGTATCGGGCAGCGAGAG+GGG	-	chr7_3:2617727-2617746	Msa1089270:CDS	60.0%
!!	CGGTGCTGTTGGTGGTGTTG+TGG	-	chr7_3:2617769-2617788	Msa1089270:CDS	60.0%
!!	GCTGTTGGTGGTGTTGTGGC+TGG	-	chr7_3:2617773-2617792	Msa1089270:CDS	60.0%
!!	TGGTGGTGTTGTGGCTGGAG+TGG	-	chr7_3:2617778-2617797	Msa1089270:CDS	60.0%
	TGTGGCTGGAGTGGCTATCG+CGG	-	chr7_3:2617787-2617806	Msa1089270:CDS	60.0%
	AGGACACACGGGTCGGACGT+GGG	-	chr7_3:2617589-2617608	Msa1089270:CDS	65.0%
!	GTGGCAGCAGGGCTTGGTAC+CGG	-	chr7_3:2617701-2617720	Msa1089270:CDS	65.0%
!	TGGCAGCAGGGCTTGGTACC+GGG	-	chr7_3:2617702-2617721	Msa1089270:CDS	65.0%
	GCGTTGTATCGGGCAGCGAG+AGG	-	chr7_3:2617725-2617744	Msa1089270:CDS	65.0%
!!	GCTGCTGTTGCCGGTGCTGT+TGG	-	chr7_3:2617758-2617777	Msa1089270:CDS	65.0%
!!	GCTGTTGCCGGTGCTGTTGG+TGG	-	chr7_3:2617761-2617780	Msa1089270:CDS	65.0%
	GTGGCTGGAGTGGCTATCGC+GGG	-	chr7_3:2617788-2617807	Msa1089270:CDS	65.0%
	TGGCTGGAGTGGCTATCGCG+GGG	-	chr7_3:2617789-2617808	Msa1089270:CDS	65.0%
	AGTGGCTATCGCGGGGAAGC+AGG	-	chr7_3:2617796-2617815	Msa1089270:CDS	65.0%
	ACCGGATGCGGCTACCTCGG+CGG	-	chr7_3:2617467-2617486	Msa1089270:CDS	70.0%
	TACCTCGGCGGCGCCATTGG+TGG	-	chr7_3:2617479-2617498	Msa1089270:CDS	70.0%
	ACCTACGCCGGCGCCACCAA+TGG	+	chr7_3:2617495-2617514	None:intergenic	70.0%
	CAGGACACACGGGTCGGACG+TGG	-	chr7_3:2617588-2617607	Msa1089270:CDS	70.0%
	GGACACACGGGTCGGACGTG+GGG	-	chr7_3:2617590-2617609	Msa1089270:CDS	70.0%
	GACACACGGGTCGGACGTGG+GGG	-	chr7_3:2617591-2617610	Msa1089270:CDS	70.0%
!	GGCAGCAGGGCTTGGTACCG+GGG	-	chr7_3:2617703-2617722	Msa1089270:CDS	70.0%
	CGCTGCCCGATACAACGCCC+CGG	+	chr7_3:2617723-2617742	None:intergenic	70.0%
	GCCGCCGAGGTAGCCGCATC+CGG	+	chr7_3:2617471-2617490	None:intergenic	75.0%
	GGCTACCTCGGCGGCGCCAT+TGG	-	chr7_3:2617476-2617495	Msa1089270:CDS	75.0%
!!	GCCATTGGTGGCGCCGGCGT+AGG	-	chr7_3:2617491-2617510	Msa1089270:CDS	75.0%
	CGGACGTGGGGGAACCGCCT+TGG	-	chr7_3:2617602-2617621	Msa1089270:CDS	75.0%
!	CGCCACCAATGGCGCCGCCG+AGG	+	chr7_3:2617484-2617503	None:intergenic	80.0%
!!!	GGCGGCGCCATTGGTGGCGC+CGG	-	chr7_3:2617485-2617504	Msa1089270:CDS	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr7_3	gene	2617278	2617844	2617278	ID=Msa1089270;Name=Msa1089270
chr7_3	mRNA	2617278	2617844	2617278	ID=Msa1089270-mRNA-1;Parent=Msa1089270;Name=Msa1089270-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|188
chr7_3	exon	2617278	2617844	2617278	ID=Msa1089270-mRNA-1:exon:1118;Parent=Msa1089270-mRNA-1
chr7_3	CDS	2617278	2617844	2617278	ID=Msa1089270-mRNA-1:cds;Parent=Msa1089270-mRNA-1

Gene Sequence

>Msa1089270

ATGGCGTTTCACTCCCCCGATCACGAATCAAACTCCGATTCCAACCCTCCAAAACGTCTCTACAATCCCTACCAAGACCTCGAAGTACCAATCCGAAACCTCTACCAGCTCCCAACCTCACCTGAACATCTCTTCATCGAGGAAGCTCAACGCAAACATCGTTCCTGGGGAGAAACCCTAACCTTTTACACCGGATGCGGCTACCTCGGCGGCGCCATTGGTGGCGCCGGCGTAGGTTTCGTCGAAGCCGTGAAATCGTTCGAGAAAGGTGATACTAATAAGCTCAGACTCAACAAGATTCTGAATTCGTCAGGACACACGGGTCGGACGTGGGGGAACCGCCTTGGTGTGATCGGGTTGTTATTCGCTGGAATTGAAAGCGGGATCACGGCGGCGAGAGATACGGACGATGTTTTCAACAGTGTGGCAGCAGGGCTTGGTACCGGGGCGTTGTATCGGGCAGCGAGAGGGGTGAGATCAGCTGCTGTTGCCGGTGCTGTTGGTGGTGTTGTGGCTGGAGTGGCTATCGCGGGGAAGCAGGCTTTGAAACGCTATGTGCCTATATGA

Protein sequence

>Msa1089270

MAFHSPDHESNSDSNPPKRLYNPYQDLEVPIRNLYQLPTSPEHLFIEEAQRKHRSWGETLTFYTGCGYLGGAIGGAGVGFVEAVKSFEKGDTNKLRLNKILNSSGHTGRTWGNRLGVIGLLFAGIESGITAARDTDDVFNSVAAGLGTGALYRAARGVRSAAVAGAVGGVVAGVAIAGKQALKRYVPI*