AlfalfaGEDB | Msa0661150

M. sativa cultivar zhongmu-4 / Msa0661150

KEGG: -
GO: GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0055044 GO:0071944
PFAM: PF02298.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0661150	AFK38628.1	94.118	187	8	1	1	187	1	184	3.36e-112	327

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0661150	sp\|Q9SK27\|ENL1_ARATH	50.286	175	75	5	11	184	15	178	6.62e-54	172

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000050	Msa0661150	0.819346	1.219159e-52	-8.615850e-47
Msa0003080	Msa0661150	0.808721	2.694339e-50	-8.615850e-47
Msa0005890	Msa0661150	0.890052	1.396181e-73	-8.615850e-47
Msa0008040	Msa0661150	0.840610	7.838338e-58	-8.615850e-47
Msa0018160	Msa0661150	0.863805	1.915552e-64	-8.615850e-47
Msa0021500	Msa0661150	0.820547	6.473909e-53	-8.615850e-47
Msa0025160	Msa0661150	0.847931	8.461544e-60	-8.615850e-47
Msa0025580	Msa0661150	0.858296	9.082389e-63	-8.615850e-47
Msa0027280	Msa0661150	0.861650	8.840156e-64	-8.615850e-47
Msa0028420	Msa0661150	0.815084	1.108030e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0661150	MtrunA17_Chr1g0150131	94.652	187	7	1	1	187	1	184	1.29e-108	308
Msa0661150	MtrunA17_Chr3g0136871	64.474	152	47	3	25	176	24	168	5.68e-64	195
Msa0661150	MtrunA17_Chr7g0256441	56.757	148	40	4	25	172	24	147	9.40e-50	157
Msa0661150	MtrunA17_Chr8g0360371	46.281	121	62	2	12	132	8	125	1.20e-35	126
Msa0661150	MtrunA17_Chr4g0041811	49.541	109	54	1	24	132	28	135	2.88e-34	120
Msa0661150	MtrunA17_Chr2g0317231	43.836	146	78	3	10	155	8	149	2.10e-33	125
Msa0661150	MtrunA17_Chr6g0458331	48.182	110	54	2	21	129	29	136	1.15e-31	112
Msa0661150	MtrunA17_Chr2g0324721	38.286	175	88	4	30	187	29	200	1.52e-29	108
Msa0661150	MtrunA17_Chr4g0040291	44.615	130	68	3	25	153	22	148	1.97e-29	107
Msa0661150	MtrunA17_Chr2g0324711	41.818	165	77	7	11	163	11	168	2.17e-29	111

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0661150	AT4G31840.1	50.000	176	77	3	12	187	11	175	2.87e-57	178
Msa0661150	AT2G25060.1	50.286	175	75	5	11	184	15	178	6.73e-55	172
Msa0661150	AT5G25090.1	53.279	122	57	0	11	132	9	130	4.02e-47	152
Msa0661150	AT4G30590.1	53.600	125	54	1	12	132	8	132	2.04e-45	148
Msa0661150	AT5G57920.3	44.079	152	82	3	26	177	53	201	1.10e-43	145
Msa0661150	AT5G57920.1	44.079	152	82	3	26	177	21	169	1.14e-43	144
Msa0661150	AT5G57920.2	44.079	152	82	3	26	177	20	168	1.24e-43	144
Msa0661150	AT2G23990.1	51.773	141	55	3	14	150	10	141	6.54e-42	140
Msa0661150	AT2G23990.2	45.625	160	55	4	14	150	10	160	3.25e-37	129
Msa0661150	AT3G20570.1	41.104	163	82	5	12	164	15	173	2.08e-35	123

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Find 55 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAGTTGCACCACTTGT+TGG	0.440200	4_3:-72377664	None:intergenic
AAAAGTGAAAATTACTTAGA+TGG	0.435763	4_3:-72376986	None:intergenic
AAAGTGAGATTTGATCATTC+TGG	0.347650	4_3:+72377475	Msa0661150:CDS
AAATTCCATTATATATGTTT+TGG	0.286437	4_3:+72377898	Msa0661150:three_prime_UTR
AATATAATAATAATATCAGA+AGG	0.608251	4_3:-72377947	None:intergenic
AATCTGATTCTCTCAACAAA+TGG	0.402045	4_3:+72376934	Msa0661150:CDS
ACAAATGGGCTTCAAGTGTT+CGG	0.440098	4_3:+72376949	Msa0661150:CDS
ACTTCAGCTGGTGAAGGTGC+TGG	0.517037	4_3:-72377607	None:intergenic
AGAAAAGGGTCATTGTGAGA+AGG	0.594361	4_3:+72377519	Msa0661150:CDS
AGAGAATCAGATTCCGAAGA+AGG	0.516269	4_3:-72376924	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CAACATTTGCAGAAAAACAA+TGG	+	chr4_3:72376788-72376807	Msa0661150:exon	30.0%
AATCTGATTCTCTCAACAAA+TGG	+	chr4_3:72376934-72376953	Msa0661150:CDS	30.0%
ATCTGATTCTCTCAACAAAT+GGG	+	chr4_3:72376935-72376954	Msa0661150:CDS	30.0%
AAAACAAAATACCAAGATGG+TGG	-	chr4_3:72377242-72377261	None:intergenic	30.0%
AAAGTGAGATTTGATCATTC+TGG	+	chr4_3:72377475-72377494	Msa0661150:CDS	30.0%
ATTAGGATTAAGATTGGGAT+TGG	+	chr4_3:72377790-72377809	Msa0661150:three_prime_UTR	30.0%
CAACATTTGCAGAAAAACAA+TGG	+	chr4_3:72376788-72376807	Msa0661150:exon	30.0%
AATCTGATTCTCTCAACAAA+TGG	+	chr4_3:72376934-72376953	Msa0661150:CDS	30.0%
ATCTGATTCTCTCAACAAAT+GGG	+	chr4_3:72376935-72376954	Msa0661150:CDS	30.0%
AAAACAAAATACCAAGATGG+TGG	-	chr4_3:72377242-72377261	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_3	gene	72376713	72377994	72376713	ID=Msa0661150;Name=Msa0661150
chr4_3	mRNA	72376713	72377994	72376713	ID=Msa0661150-mRNA-1;Parent=Msa0661150;Name=Msa0661150-mRNA-1;_AED=0.00;_eAED=0.00;_QI=94\|1\|1\|1\|1\|1\|2\|250\|187
chr4_3	exon	72376713	72376993	72376713	ID=Msa0661150-mRNA-1:exon:21663;Parent=Msa0661150-mRNA-1
chr4_3	exon	72377368	72377994	72377368	ID=Msa0661150-mRNA-1:exon:21664;Parent=Msa0661150-mRNA-1
chr4_3	five_prime_UTR	72376713	72376806	72376713	ID=Msa0661150-mRNA-1:five_prime_utr;Parent=Msa0661150-mRNA-1
chr4_3	CDS	72376807	72376993	72376807	ID=Msa0661150-mRNA-1:cds;Parent=Msa0661150-mRNA-1
chr4_3	CDS	72377368	72377744	72377368	ID=Msa0661150-mRNA-1:cds;Parent=Msa0661150-mRNA-1
chr4_3	three_prime_UTR	72377745	72377994	72377745	ID=Msa0661150-mRNA-1:three_prime_utr;Parent=Msa0661150-mRNA-1

Gene Sequence

>Msa0661150

ATGGCTGGTTCGTCTTCTTCATCATCTTGTTCTCTTTTGGTTCTTTTTGTTCTCTTTGGTTGTGCTTTTGCAGCTAAGGATATCTTGCTTGGTGGCAAAACTGATGCATGGAAGGTTCCTTCTTCGGAATCTGATTCTCTCAACAAATGGGCTTCAAGTGTTCGGTTTCAAGTTGGAGACCATCTAATTTGGAAATATGAAGCTGGAAAAGATTCTGTTTTGCAAGTGAGTAAGGAAGATTATGATAGCTGCAACACCTCAAAACCCATCAAACACTACAATGATGGGAACACTAAAGTGAGATTTGATCATTCTGGTCCATACTACTACATCAGTGGAGAAAAGGGTCATTGTGAGAAGGGTCAGAAGCTTACTGTGGTTGTCATGAGTCTAAGGGGGGGAAGTAGGCCCATTGTTGCTTTTTCACCAGCACCTTCACCAGCTGAAGTTGATGGTCCAGCTGGCTCAGCTGTTGCTCCAGCTCCAACAAGTGGTGCAACTGTTTTGCAAGGTGGTGGTGTATTTGTGGCTGTAGGAGTGTTTGTTGCCATGTGGCTTTTCTGA

Protein sequence

>Msa0661150

MAGSSSSSSCSLLVLFVLFGCAFAAKDILLGGKTDAWKVPSSESDSLNKWASSVRFQVGDHLIWKYEAGKDSVLQVSKEDYDSCNTSKPIKHYNDGNTKVRFDHSGPYYYISGEKGHCEKGQKLTVVVMSLRGGSRPIVAFSPAPSPAEVDGPAGSAVAPAPTSGATVLQGGGVFVAVGVFVAMWLF*