AlfalfaGEDB | Msa0851160

M. sativa cultivar zhongmu-4 / Msa0851160

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0851160	XP_003615261.2	98.862	1230	13	1	1	1229	1	1230	0.0	2534

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0851160	G7KBI4	98.862	1230	13	1	1	1229	1	1230	0.0	2534

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0002420	Msa0851160	0.833206	6.091258e-56	-8.615850e-47
Msa0254930	Msa0851160	0.803408	3.537051e-49	-8.615850e-47
Msa0309720	Msa0851160	0.805294	1.430758e-49	-8.615850e-47
Msa0389690	Msa0851160	0.808084	3.684793e-50	-8.615850e-47
Msa0416020	Msa0851160	0.836165	1.098059e-56	-8.615850e-47
Msa0508180	Msa0851160	0.841209	5.459192e-58	-8.615850e-47
Msa0519760	Msa0851160	0.813968	1.956381e-51	-8.615850e-47
Msa0519840	Msa0851160	0.812343	4.446025e-51	-8.615850e-47
Msa0624900	Msa0851160	0.819588	1.073333e-52	-8.615850e-47
Msa0641990	Msa0851160	0.812395	4.332572e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0851160	MtrunA17_Chr5g0427251	98.862	1230	13	1	1	1229	1	1230	0.0	2534
Msa0851160	MtrunA17_Chr5g0448351	45.393	1042	492	15	214	1229	207	1197	0.0	903
Msa0851160	MtrunA17_Chr5g0401841	61.667	60	14	2	644	694	4	63	3.05e-13	65.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa0851160	AT4G24610.1	62.648	1186	395	12	47	1229	10	1150	1495
Msa0851160	AT4G24610.3	62.679	1187	397	12	47	1229	10	1154	1493
Msa0851160	AT4G24610.4	62.595	1187	395	13	47	1229	10	1151	1491
Msa0851160	AT4G24610.2	62.626	1188	397	13	47	1229	10	1155	1489
Msa0851160	AT5G65440.1	53.846	1014	449	7	229	1229	43	1050	1136
Msa0851160	AT5G65440.8	53.846	1014	449	7	229	1229	43	1050	1136
Msa0851160	AT5G65440.9	53.846	1014	449	7	229	1229	43	1050	1136
Msa0851160	AT5G65440.6	56.550	939	401	4	292	1229	159	1091	1133
Msa0851160	AT5G65440.4	56.550	939	401	4	292	1229	159	1091	1133
Msa0851160	AT5G65440.7	56.550	939	401	4	292	1229	159	1091	1133

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Find 298 sgRNAs with CRISPR-Local

Find 481 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTTATTGGCAAGATGA+AGG	0.526439	5_4:+41242165	Msa0851160:CDS
AAACCCTGTGCATAAATCTT+AGG	0.320391	5_4:+41244543	Msa0851160:CDS
AAACTGTTGTCCTTGTGATT+AGG	0.380356	5_4:-41247366	None:intergenic
AAAGTTCAGGAACTACTTGC+AGG	0.398147	5_4:+41246175	Msa0851160:CDS
AAATAAAGAGGGTTATTAAG+GGG	0.598297	5_4:+41247420	Msa0851160:three_prime_UTR
AAATCATCTCCAAGACTATC+TGG	0.365820	5_4:-41242950	None:intergenic
AACAGCAGATTGCCTTAACT+TGG	0.461204	5_4:+41243681	Msa0851160:CDS
AACAGTCTGACAAAGTACGC+TGG	0.679236	5_4:+41243140	Msa0851160:intron
AACTTCCTTGAGCCACCAAG+AGG	0.658392	5_4:+41243747	Msa0851160:CDS
AAGAGGAAGTTTATGGTGGA+AGG	0.571425	5_4:+41240612	Msa0851160:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CTCTCTAAGCTTAATATCAA+AGG	+	chr5_4:41239801-41239820	Msa0851160:five_prime_UTR	30.0%
GTTTACTTGCTTAATCAGAA+AGG	-	chr5_4:41239832-41239851	None:intergenic	30.0%
GATTTGAGAGAGAGAAAAAA+AGG	-	chr5_4:41239855-41239874	None:intergenic	30.0%
TTCTGCCAAAATAACTACAT+TGG	+	chr5_4:41240328-41240347	Msa0851160:intron	30.0%
TCACTTTCACACATTGTTAT+TGG	+	chr5_4:41241195-41241214	Msa0851160:intron	30.0%
ATAGCACAAATCACAAAGTT+AGG	-	chr5_4:41241296-41241315	None:intergenic	30.0%
GTTAAGAACTCAATATTGAG+AGG	-	chr5_4:41241567-41241586	None:intergenic	30.0%
TCTTTACTTAGAGATGCATT+TGG	+	chr5_4:41241971-41241990	Msa0851160:CDS	30.0%
CTTTACTTAGAGATGCATTT+GGG	+	chr5_4:41241972-41241991	Msa0851160:CDS	30.0%
AAAACTTATTGGCAAGATGA+AGG	+	chr5_4:41242165-41242184	Msa0851160:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_4	gene	41239733	41247734	41239733	ID=Msa0851160;Name=Msa0851160
chr5_4	mRNA	41239733	41247734	41239733	ID=Msa0851160-mRNA-1;Parent=Msa0851160;Name=Msa0851160-mRNA-1;_AED=0.12;_eAED=0.12;_QI=358\|1\|1\|1\|1\|1\|22\|336\|1229
chr5_4	exon	41239733	41240138	41239733	ID=Msa0851160-mRNA-1:exon:12988;Parent=Msa0851160-mRNA-1
chr5_4	exon	41240410	41241006	41240410	ID=Msa0851160-mRNA-1:exon:12989;Parent=Msa0851160-mRNA-1
chr5_4	exon	41241328	41241536	41241328	ID=Msa0851160-mRNA-1:exon:12990;Parent=Msa0851160-mRNA-1
chr5_4	exon	41241697	41241768	41241697	ID=Msa0851160-mRNA-1:exon:12991;Parent=Msa0851160-mRNA-1
chr5_4	exon	41241859	41241993	41241859	ID=Msa0851160-mRNA-1:exon:12992;Parent=Msa0851160-mRNA-1
chr5_4	exon	41242075	41242193	41242075	ID=Msa0851160-mRNA-1:exon:12993;Parent=Msa0851160-mRNA-1
chr5_4	exon	41242278	41242583	41242278	ID=Msa0851160-mRNA-1:exon:12994;Parent=Msa0851160-mRNA-1
chr5_4	exon	41242778	41242865	41242778	ID=Msa0851160-mRNA-1:exon:12995;Parent=Msa0851160-mRNA-1
chr5_4	exon	41242946	41243051	41242946	ID=Msa0851160-mRNA-1:exon:12996;Parent=Msa0851160-mRNA-1
chr5_4	exon	41243145	41243258	41243145	ID=Msa0851160-mRNA-1:exon:12997;Parent=Msa0851160-mRNA-1
chr5_4	exon	41243375	41243538	41243375	ID=Msa0851160-mRNA-1:exon:12998;Parent=Msa0851160-mRNA-1
chr5_4	exon	41243648	41243768	41243648	ID=Msa0851160-mRNA-1:exon:12999;Parent=Msa0851160-mRNA-1
chr5_4	exon	41244107	41244331	41244107	ID=Msa0851160-mRNA-1:exon:13000;Parent=Msa0851160-mRNA-1
chr5_4	exon	41244425	41244615	41244425	ID=Msa0851160-mRNA-1:exon:13001;Parent=Msa0851160-mRNA-1
chr5_4	exon	41244936	41245103	41244936	ID=Msa0851160-mRNA-1:exon:13002;Parent=Msa0851160-mRNA-1
chr5_4	exon	41245220	41245325	41245220	ID=Msa0851160-mRNA-1:exon:13003;Parent=Msa0851160-mRNA-1
chr5_4	exon	41245455	41245592	41245455	ID=Msa0851160-mRNA-1:exon:13004;Parent=Msa0851160-mRNA-1
chr5_4	exon	41245771	41245932	41245771	ID=Msa0851160-mRNA-1:exon:13005;Parent=Msa0851160-mRNA-1
chr5_4	exon	41246023	41246223	41246023	ID=Msa0851160-mRNA-1:exon:13006;Parent=Msa0851160-mRNA-1
chr5_4	exon	41246649	41246843	41246649	ID=Msa0851160-mRNA-1:exon:13007;Parent=Msa0851160-mRNA-1
chr5_4	exon	41246946	41247017	41246946	ID=Msa0851160-mRNA-1:exon:13008;Parent=Msa0851160-mRNA-1
chr5_4	exon	41247246	41247734	41247246	ID=Msa0851160-mRNA-1:exon:13009;Parent=Msa0851160-mRNA-1
chr5_4	five_prime_UTR	41239733	41240090	41239733	ID=Msa0851160-mRNA-1:five_prime_utr;Parent=Msa0851160-mRNA-1
chr5_4	CDS	41240091	41240138	41240091	ID=Msa0851160-mRNA-1:cds;Parent=Msa0851160-mRNA-1
chr5_4	CDS	41240410	41241006	41240410	ID=Msa0851160-mRNA-1:cds;Parent=Msa0851160-mRNA-1
chr5_4	CDS	41241328	41241536	41241328	ID=Msa0851160-mRNA-1:cds;Parent=Msa0851160-mRNA-1
chr5_4	CDS	41241697	41241768	41241697	ID=Msa0851160-mRNA-1:cds;Parent=Msa0851160-mRNA-1
chr5_4	CDS	41241859	41241993	41241859	ID=Msa0851160-mRNA-1:cds;Parent=Msa0851160-mRNA-1
chr5_4	CDS	41242075	41242193	41242075	ID=Msa0851160-mRNA-1:cds;Parent=Msa0851160-mRNA-1
chr5_4	CDS	41242278	41242583	41242278	ID=Msa0851160-mRNA-1:cds;Parent=Msa0851160-mRNA-1
chr5_4	CDS	41242778	41242865	41242778	ID=Msa0851160-mRNA-1:cds;Parent=Msa0851160-mRNA-1
chr5_4	CDS	41242946	41243051	41242946	ID=Msa0851160-mRNA-1:cds;Parent=Msa0851160-mRNA-1
chr5_4	CDS	41243145	41243258	41243145	ID=Msa0851160-mRNA-1:cds;Parent=Msa0851160-mRNA-1
chr5_4	CDS	41243375	41243538	41243375	ID=Msa0851160-mRNA-1:cds;Parent=Msa0851160-mRNA-1
chr5_4	CDS	41243648	41243768	41243648	ID=Msa0851160-mRNA-1:cds;Parent=Msa0851160-mRNA-1
chr5_4	CDS	41244107	41244331	41244107	ID=Msa0851160-mRNA-1:cds;Parent=Msa0851160-mRNA-1
chr5_4	CDS	41244425	41244615	41244425	ID=Msa0851160-mRNA-1:cds;Parent=Msa0851160-mRNA-1
chr5_4	CDS	41244936	41245103	41244936	ID=Msa0851160-mRNA-1:cds;Parent=Msa0851160-mRNA-1
chr5_4	CDS	41245220	41245325	41245220	ID=Msa0851160-mRNA-1:cds;Parent=Msa0851160-mRNA-1
chr5_4	CDS	41245455	41245592	41245455	ID=Msa0851160-mRNA-1:cds;Parent=Msa0851160-mRNA-1
chr5_4	CDS	41245771	41245932	41245771	ID=Msa0851160-mRNA-1:cds;Parent=Msa0851160-mRNA-1
chr5_4	CDS	41246023	41246223	41246023	ID=Msa0851160-mRNA-1:cds;Parent=Msa0851160-mRNA-1
chr5_4	CDS	41246649	41246843	41246649	ID=Msa0851160-mRNA-1:cds;Parent=Msa0851160-mRNA-1
chr5_4	CDS	41246946	41247017	41246946	ID=Msa0851160-mRNA-1:cds;Parent=Msa0851160-mRNA-1
chr5_4	CDS	41247246	41247398	41247246	ID=Msa0851160-mRNA-1:cds;Parent=Msa0851160-mRNA-1
chr5_4	three_prime_UTR	41247399	41247734	41247399	ID=Msa0851160-mRNA-1:three_prime_utr;Parent=Msa0851160-mRNA-1

Gene Sequence

>Msa0851160

ATGTTCACTGAAGGACTTGACAAAAACGCACTTAGATGGGTCAGAGAGAAGGAAGTGCCATTCTCAAACACAGCAATGAGATCTCGTGATCCGATTAGTGGAATGAAAAGTGGCGGCGGTAGAGGGTTTGGATTACCACCACCATCAAAATTTAGGAGTGGTCATCTTCCTGCTAATAAGTTACCTGTCTCGGCGGTTGAGACATTCGATAGCGGTTCAAATAGTGATATGGACGCCAGCGTTGATTCGGAAGAGGAAGTTTATGGTGGAAGGTATTCTTTGGATTCATCACCACAAGACAACAGAGTCCCCAATGGTGCTGCTAAGAGATATGGGAATGTTGCTCAGATGCCACGGTCTCGGTATGCGAGTGATTACACGTTCTCTGATGTTAGTTCGTCCAGAGAAACACTTACTGGAAGACAAGGAATGACGAGGGATCCGGTAATGAGGAGTGCTGCTAATGGAAGGCAGAATGGTTTCACTGAGGATGATTCGTCGGATTCTGCGGCTAGCTCAGAATTTTCTACCACACAAGTAGGGAGTAGTATCAATGGCACTCTTCCAAAAAGCAGGGCTTATATGTCAGCAGGGTATGCTTCTAGTGTACCTTCAAGGATGAATGTTCAAAGTTCTGCAGAAAAGAGTGGAAGGTTATCTGATGACGAGGACGAAGACATTCCGAGTGCACCCCCATTTTGTGGTTCTACTCAAGAGATAAGACAAACTAACGAGGAAATTCCAACTTCTGCAACACGTAGTACTCCAAATAAAGCAGAATCAAGTACTTTGAAATCTGTATCCAGGGATAAGTTAGAGAACCATGGAGATGCAAGCTCGGAAAAATTCGTTAGAACTGCTACTGGTTCAGAGGGTGCTGCATCTTCCAATTCACAACCACCTCGCCTTCCAACATTTCATGCAAGTGCTCTTGGGCCATGGTATGCAGTAATTGCATACGACGCCTGTGCGCGTCTTTGCCTTCACGCTTGGGCGATGCAGTGTATGGAAGCTCCAATGTTTTTGGAAAATGAATGCTCTTTACTTAGAGATGCATTTGGATTGCGACAAGTGCTTCTTCAACCAGAAGAGGAGCTAATGGTGAAATGCAATGGAGAACTTTCTAGTGAGGGTGTTGCTCCTAAACTGAAAAAACTTATTGGCAAGATGAAGGTGCAAGTACGCAAAGTCAAAGTGGGGCTCGACCCTCCTACTGGATGTAGCATGTCATCAATAGTGACACATAAAATTAAAATGGATTCAATGCGACACCACTTCTCAAATTTGCAGTCAAAACTATCTTCTGGTTGGCACGCTCTTAGGAAAGTTCGGTTTGTACCACATTTGCCTGCAAATGGTTCCCTGACACATAAAAGCTTGGCATATGTGCATGCTAGCACACGCTATATACAACAAGTCTCTGGACTCCTAAAAGTTGGTGTAACAACTTTGCGGAATAGTTCATCCTCCTATGAAGCTGTGCAAGAGACATTCACGTGTTTCTTACGACTCAAAAGTGTAGTTGAAGAAGACGCCATTAGGTTGCAGCCTGGATCCAGTGAAGTCCATATGTTTTTTCCAGATAGTCTTGGAGATGATTTGCTCATTGAAGTTCAAGATTCCAAGGGAAAGCATTTTGGGCGTGTTCTTGTCCAAGTGGCTGCTATCGCTGATAATCCGTCTGACAAAGTACGCTGGTGGAATGTTTACCGTGAACCAGACCATGAGCTTGTTGGCAAGATACAGCTTTATATACTCTATTCAACCAGTGCAGATGACAATAGTCATCTTAAGTGTGGTTCTGTAGCGGAGACAGTTGCATATGACCTGGTTCTAGAAGTTGCAATGAAAGTTCAGGGCTTTCAGCAAAGGAACCTAGAATTACATGGTCCATGGAAATGGCTCTTGACAGAGTTTGCTTCATATTATGGTGTGTCTGAGATTTATACGAAGCTGAGATATCTATCGTATGTCATGGATGTTGCCACACCAACAGCAGATTGCCTTAACTTGGTATATAATTTGCTTGCACCTGTTATCATGAAGGGAAATAGCAAAACTTCCTTGAGCCACCAAGAGAATCGACTACTAGGAGAGACCAAGGACGAAATTGAACAGATACTCACACTTACATTTGAGAATTACAAGTCACTTGATGAGTCATCTTTCTCTGGAATCGTCGAGGTTTTCAGACCAGCATCCAGTCATGCAGCTCCTGCCTTGGAACCGGCTGTTAAACTTTACAAGCTTCTTCATGATATCTTATCTCCTGAGGCCCAAACCAGTTTCTGTCACTACTTCCAGGTTGCGGCAAAAAAGAGGGCTAGAAGGCACCTTTCTGACACAGATGAATATATTGCACAGAATAATGAAAGCTGTTTGATGGATCCTTTGACCATGTCTACCGCTTACCAGAAGATGAAAACCCTGTGCATAAATCTTAGGAATGAGATCTATGCTGATATTCAGATTCATAATCAAAATATACTTCCTAGCTTTGTAGACCTGCCAAATCTTTCTGCTTCCATATACAGCACAGAGCTTTGCAATAGATTACGAGCCTTCCTCATATCTTGTCCACCGACAGGTCCATCATCACCAGTAGCAGAACTTGTCATTGCAACATCCGATTTTCAAAGAGATCTTTCAGGCTGGAACATTAATCCTATCAAAGGTGGAGTGGATGCAAAAGAGTTGTTCCATTTGTATATTCTTGTTTGGATCCAAGATAAACGTCTATCTTTGCTCGAGTCGTGCAAATTAGACAAGGTGAAATGGTCTGGTGTTAGAACACAACATTCTACTACTCCCTTTGTTGATGACATGTATGAGCGGCTGAAGGAAACATTGACAGACTATGAGGTCATCATTTGCCGCTGGCCAGAATATACCTTGGTTCTAGAAAATGCAATAGCTGATATTGAGAAGGCGATTGTGGAAGCTTTAGATAAACAGTATGCGGATGTATTAGCTCCTCTTAAAGATAGCATGGCACCTAAGAAATTTGGGCTTAAATATGTCCAGAAACTTGCCAAACGGTCTACATGTGCCTATGTGGTTCCTGAGGAGGTTGGAATTCTTTTGAATTCCTTGAAGAGGATGCTCGACATCTTACGTCCAAGGATTGAATCACAGTTCAAATCATGGGCTTCTTGCTTACCAAATGCTGGAAACACAGCACCTGGTGAGCGACTTAGCGAAGTGACCGTGATGCTGAGAGCAAAGTTCAGGAACTACTTGCAGGCTATTGTGGAGAAACTCGTGGAAAATACAAAGTTACAGAATGCTACAAAATTAAAGAAGATTCTTCAAGATTCAAAGGAAACAGTGGTAGAGTCTGATTTAAAAAGTAGAATGCAACCACTGAAAGAGCAGTTAGCTAGTACCATAAGCTATCTTCACAGTATTTGTGAAACTCATGTTTTCATTGCCATCTGTCGAGGTTATTGGGACCGAATGGGACAGGAAATTTTGAGTTTCTTGGAGAACAGAAAAGAGAATAGATCATGGTATAAAGGTTCAAGGGTGGCTGTTTCTGTTCTGGATGATACATTTGCCTCACAGATGCAACAACTTCTAGGAAATGCGATACAAGAGAAAGACATGGAGGCACCTAGATGCATCATGGAGGTTCGTTCTATGCTATGTAAAGACGCTCCTAATCACAAGGACAACAGTTTTTACTATTAG

Protein sequence

>Msa0851160

MFTEGLDKNALRWVREKEVPFSNTAMRSRDPISGMKSGGGRGFGLPPPSKFRSGHLPANKLPVSAVETFDSGSNSDMDASVDSEEEVYGGRYSLDSSPQDNRVPNGAAKRYGNVAQMPRSRYASDYTFSDVSSSRETLTGRQGMTRDPVMRSAANGRQNGFTEDDSSDSAASSEFSTTQVGSSINGTLPKSRAYMSAGYASSVPSRMNVQSSAEKSGRLSDDEDEDIPSAPPFCGSTQEIRQTNEEIPTSATRSTPNKAESSTLKSVSRDKLENHGDASSEKFVRTATGSEGAASSNSQPPRLPTFHASALGPWYAVIAYDACARLCLHAWAMQCMEAPMFLENECSLLRDAFGLRQVLLQPEEELMVKCNGELSSEGVAPKLKKLIGKMKVQVRKVKVGLDPPTGCSMSSIVTHKIKMDSMRHHFSNLQSKLSSGWHALRKVRFVPHLPANGSLTHKSLAYVHASTRYIQQVSGLLKVGVTTLRNSSSSYEAVQETFTCFLRLKSVVEEDAIRLQPGSSEVHMFFPDSLGDDLLIEVQDSKGKHFGRVLVQVAAIADNPSDKVRWWNVYREPDHELVGKIQLYILYSTSADDNSHLKCGSVAETVAYDLVLEVAMKVQGFQQRNLELHGPWKWLLTEFASYYGVSEIYTKLRYLSYVMDVATPTADCLNLVYNLLAPVIMKGNSKTSLSHQENRLLGETKDEIEQILTLTFENYKSLDESSFSGIVEVFRPASSHAAPALEPAVKLYKLLHDILSPEAQTSFCHYFQVAAKKRARRHLSDTDEYIAQNNESCLMDPLTMSTAYQKMKTLCINLRNEIYADIQIHNQNILPSFVDLPNLSASIYSTELCNRLRAFLISCPPTGPSSPVAELVIATSDFQRDLSGWNINPIKGGVDAKELFHLYILVWIQDKRLSLLESCKLDKVKWSGVRTQHSTTPFVDDMYERLKETLTDYEVIICRWPEYTLVLENAIADIEKAIVEALDKQYADVLAPLKDSMAPKKFGLKYVQKLAKRSTCAYVVPEEVGILLNSLKRMLDILRPRIESQFKSWASCLPNAGNTAPGERLSEVTVMLRAKFRNYLQAIVEKLVENTKLQNATKLKKILQDSKETVVESDLKSRMQPLKEQLASTISYLHSICETHVFIAICRGYWDRMGQEILSFLENRKENRSWYKGSRVAVSVLDDTFASQMQQLLGNAIQEKDMEAPRCIMEVRSMLCKDAPNHKDNSFYY*