AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0859120

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0859120 MtrunA17_Chr5g0437861 99.254 134 1 0 1 134 1 134 3.59e-97 275
Msa0859120 MtrunA17_Chr3g0085831 33.136 169 75 4 4 134 1 169 7.24e-21 82.8
Msa0859120 MtrunA17_Chr7g0256851 44.872 78 38 2 1 78 1 73 1.97e-15 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0859120 AT2G41430.5 29.940 167 80 4 1 134 1 163 6.92e-15 67.8
Msa0859120 AT2G41430.4 29.940 167 80 4 1 134 1 163 6.92e-15 67.8
Msa0859120 AT2G41430.2 29.940 167 80 4 1 134 1 163 6.92e-15 67.8
Msa0859120 AT2G41430.1 29.940 167 80 4 1 134 1 163 6.92e-15 67.8
Msa0859120 AT2G41430.3 47.541 61 29 1 1 61 1 58 4.15e-14 64.7
Msa0859120 AT4G14270.1 48.485 66 26 2 12 75 10 69 1.69e-13 63.5
Msa0859120 AT4G14270.2 48.485 66 26 2 12 75 10 69 1.69e-13 63.5

Find 58 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGAGTTATGATTTGATTTC+AGG 0.153500 5_4:+57300388 Msa0859120:three_prime_UTR
CCCCTTGGTTCCGTGATTAC+TGG 0.164334 5_4:+57299836 Msa0859120:CDS
TGGGAAAGAGTTGATGAAAT+TGG 0.243769 5_4:+57300183 Msa0859120:CDS
TTCATCAGCCCAGGTAGATT+TGG 0.348718 5_4:+57300305 Msa0859120:exon
ACCTCGTTCATTCCTCCCCT+TGG 0.371694 5_4:+57299821 Msa0859120:CDS
TCCATTGATGTGTTTGTTGT+TGG 0.371834 5_4:-57299691 None:intergenic
AAATCAGAAAACGAGTCATT+TGG 0.381419 5_4:-57299892 None:intergenic
GGGAAAGAGTTGATGAAATT+GGG 0.389970 5_4:+57300184 Msa0859120:CDS
TTGGATAGTTCTGAGGATCT+TGG 0.417299 5_4:-57299873 None:intergenic
TCAAGGTTGAAAATTTGTTA+AGG 0.423651 5_4:+57300605 Msa0859120:three_prime_UTR
GAGGATCTTGGAAGCGTTCA+CGG 0.427509 5_4:-57299861 None:intergenic
AGAGTTATGATTTGATTTCA+GGG 0.438755 5_4:+57300389 Msa0859120:three_prime_UTR
GTTGTTGTTGTAATGTTACT+TGG 0.439043 5_4:+57300539 Msa0859120:three_prime_UTR
GGGACGAACATTGGAGCGTT+GGG 0.452927 5_4:-57299754 None:intergenic
TGAAATTGGGATCGTTGAAA+TGG 0.456391 5_4:+57300197 Msa0859120:CDS
ACTCTGGGCTTCACAATCTT+CGG 0.460415 5_4:-57300268 None:intergenic
TGGGACGAACATTGGAGCGT+TGG 0.498591 5_4:-57299755 None:intergenic
ACCAACAACAAACACATCAA+TGG 0.501895 5_4:+57299690 Msa0859120:exon
TTTGGTGGCAAAGTGGAAAC+TGG 0.504496 5_4:+57300323 Msa0859120:three_prime_UTR
ATCAGCCCAGGTAGATTTGG+TGG 0.507054 5_4:+57300308 Msa0859120:exon
CTTGTAGATCTCAATGATCA+AGG 0.510028 5_4:+57300588 Msa0859120:three_prime_UTR
GTGCGTGTAGAACACGAAGA+TGG 0.520016 5_4:+57300163 Msa0859120:intron
ACTTTGCCACCAAATCTACC+TGG 0.520178 5_4:-57300314 None:intergenic
GGGGTTCAATTTCATGAACA+TGG 0.523834 5_4:-57299942 None:intergenic
GGAGCCTTCTCAGCATACCT+TGG 0.524540 5_4:-57300247 None:intergenic
TAAGCTAGTGGGACGAACAT+TGG 0.525972 5_4:-57299763 None:intergenic
ATAGCCCGTGGGCTCACTCT+GGG 0.531409 5_4:-57300283 None:intergenic
GTTGGGATTCAACGAGGAAG+AGG 0.533447 5_4:-57299737 None:intergenic
ATGAAGAAAACAAGATAAGT+GGG 0.542611 5_4:-57299666 None:intergenic
GCCAGTAATCACGGAACCAA+GGG 0.550209 5_4:-57299837 None:intergenic
AAAACAAGATAAGTGGGTGA+TGG 0.556508 5_4:-57299660 None:intergenic
TGCGTGTAGAACACGAAGAT+GGG 0.556627 5_4:+57300164 Msa0859120:intron
AGTTCCAAGGTATGCTGAGA+AGG 0.558393 5_4:+57300243 Msa0859120:CDS
TGTTACTTGGAAAATTGTGT+AGG 0.560893 5_4:+57300552 Msa0859120:three_prime_UTR
GATGAAGAAAACAAGATAAG+TGG 0.563459 5_4:-57299667 None:intergenic
AACGAGGTTCCACCATTCAT+CGG 0.566948 5_4:-57299806 None:intergenic
TGAAGCCCAGAGTGAGCCCA+CGG 0.571247 5_4:+57300278 Msa0859120:CDS
TGGAGGATTTCTCCGATGAA+TGG 0.573834 5_4:+57299794 Msa0859120:CDS
TTGAAATGGAGAGGATCTGA+TGG 0.578122 5_4:+57300211 Msa0859120:CDS
CACGGGCTATTCATCAGCCC+AGG 0.580678 5_4:+57300296 Msa0859120:CDS
ATGGATGGGCTGAAGTTCCA+AGG 0.594708 5_4:+57300230 Msa0859120:CDS
TTGGGATCGTTGAAATGGAG+AGG 0.600872 5_4:+57300202 Msa0859120:CDS
AGGTAGATTTGGTGGCAAAG+TGG 0.603013 5_4:+57300316 Msa0859120:exon
GAGTCATTTGGATAGTTCTG+AGG 0.610454 5_4:-57299880 None:intergenic
AGCCAGTAATCACGGAACCA+AGG 0.611593 5_4:-57299838 None:intergenic
AGGATTTCTCCGATGAATGG+TGG 0.617511 5_4:+57299797 Msa0859120:CDS
AATGGAGAGGATCTGATGGA+TGG 0.618481 5_4:+57300215 Msa0859120:CDS
ATGGAGAGGATCTGATGGAT+GGG 0.622633 5_4:+57300216 Msa0859120:CDS
CTTTGCCACCAAATCTACCT+GGG 0.625860 5_4:-57300313 None:intergenic
GAAGCCCAGAGTGAGCCCAC+GGG 0.627082 5_4:+57300279 Msa0859120:CDS
GTTCACGGAGCCAGTAATCA+CGG 0.629661 5_4:-57299846 None:intergenic
TCCACAGTACGATAAGCTAG+TGG 0.629696 5_4:-57299775 None:intergenic
CCCACTAGCTTATCGTACTG+TGG 0.640804 5_4:+57299774 Msa0859120:CDS
GTAATCACGGAACCAAGGGG+AGG 0.688535 5_4:-57299833 None:intergenic
ACTAGCTTATCGTACTGTGG+AGG 0.702577 5_4:+57299777 Msa0859120:CDS
TGGAGCGTTGGGATTCAACG+AGG 0.718972 5_4:-57299743 None:intergenic
CCAGTAATCACGGAACCAAG+GGG 0.718986 5_4:-57299836 None:intergenic
ACCAAGGGGAGGAATGAACG+AGG 0.740764 5_4:-57299822 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTATGTGATTAATTTTGA+TGG + chr5_4:57300110-57300129 Msa0859120:intron 15.0%
!!! TAACTCTAGTTATTTTTTTA+CGG - chr5_4:57300376-57300395 None:intergenic 15.0%
!!! ATTTATGTGATTAATTTTGA+TGG + chr5_4:57300110-57300129 Msa0859120:intron 15.0%
!!! TAACTCTAGTTATTTTTTTA+CGG - chr5_4:57300376-57300395 None:intergenic 15.0%
!! AATAAAGTAAAATAGCTTAC+GGG - chr5_4:57299965-57299984 None:intergenic 20.0%
!! CAATAAAGTAAAATAGCTTA+CGG - chr5_4:57299966-57299985 None:intergenic 20.0%
!!! ATTTTGATGGATTAATCTAA+TGG + chr5_4:57300123-57300142 Msa0859120:intron 20.0%
!!! AACTCTAGTTATTTTTTTAC+GGG - chr5_4:57300375-57300394 None:intergenic 20.0%
!! AATAAAGTAAAATAGCTTAC+GGG - chr5_4:57299965-57299984 None:intergenic 20.0%
!! CAATAAAGTAAAATAGCTTA+CGG - chr5_4:57299966-57299985 None:intergenic 20.0%
!!! ATTTTGATGGATTAATCTAA+TGG + chr5_4:57300123-57300142 Msa0859120:intron 20.0%
!!! AACTCTAGTTATTTTTTTAC+GGG - chr5_4:57300375-57300394 None:intergenic 20.0%
! ATGAAGAAAACAAGATAAGT+GGG - chr5_4:57299669-57299688 None:intergenic 25.0%
!!! GTTTTCTGATTTTGAAATGA+TGG + chr5_4:57299903-57299922 Msa0859120:CDS 25.0%
! ATAAAGTAAAATAGCTTACG+GGG - chr5_4:57299964-57299983 None:intergenic 25.0%
!! TAGAGTTATGATTTGATTTC+AGG + chr5_4:57300388-57300407 Msa0859120:three_prime_UTR 25.0%
!! AGAGTTATGATTTGATTTCA+GGG + chr5_4:57300389-57300408 Msa0859120:three_prime_UTR 25.0%
!!! TCAAGGTTGAAAATTTGTTA+AGG + chr5_4:57300605-57300624 Msa0859120:three_prime_UTR 25.0%
! ATGAAGAAAACAAGATAAGT+GGG - chr5_4:57299669-57299688 None:intergenic 25.0%
!!! GTTTTCTGATTTTGAAATGA+TGG + chr5_4:57299903-57299922 Msa0859120:CDS 25.0%
! ATAAAGTAAAATAGCTTACG+GGG - chr5_4:57299964-57299983 None:intergenic 25.0%
!! TAGAGTTATGATTTGATTTC+AGG + chr5_4:57300388-57300407 Msa0859120:three_prime_UTR 25.0%
!! AGAGTTATGATTTGATTTCA+GGG + chr5_4:57300389-57300408 Msa0859120:three_prime_UTR 25.0%
!!! TCAAGGTTGAAAATTTGTTA+AGG + chr5_4:57300605-57300624 Msa0859120:three_prime_UTR 25.0%
GATGAAGAAAACAAGATAAG+TGG - chr5_4:57299670-57299689 None:intergenic 30.0%
AAATCAGAAAACGAGTCATT+TGG - chr5_4:57299895-57299914 None:intergenic 30.0%
! AATGGATCTGATGTTTGTTT+TGG + chr5_4:57300141-57300160 Msa0859120:intron 30.0%
! GTTGTTGTTGTAATGTTACT+TGG + chr5_4:57300539-57300558 Msa0859120:three_prime_UTR 30.0%
! TGTTACTTGGAAAATTGTGT+AGG + chr5_4:57300552-57300571 Msa0859120:three_prime_UTR 30.0%
GATGAAGAAAACAAGATAAG+TGG - chr5_4:57299670-57299689 None:intergenic 30.0%
AAATCAGAAAACGAGTCATT+TGG - chr5_4:57299895-57299914 None:intergenic 30.0%
! AATGGATCTGATGTTTGTTT+TGG + chr5_4:57300141-57300160 Msa0859120:intron 30.0%
! GTTGTTGTTGTAATGTTACT+TGG + chr5_4:57300539-57300558 Msa0859120:three_prime_UTR 30.0%
! TGTTACTTGGAAAATTGTGT+AGG + chr5_4:57300552-57300571 Msa0859120:three_prime_UTR 30.0%
AAAACAAGATAAGTGGGTGA+TGG - chr5_4:57299663-57299682 None:intergenic 35.0%
ACCAACAACAAACACATCAA+TGG + chr5_4:57299690-57299709 Msa0859120:exon 35.0%
!! TCCATTGATGTGTTTGTTGT+TGG - chr5_4:57299694-57299713 None:intergenic 35.0%
!! TGGGAAAGAGTTGATGAAAT+TGG + chr5_4:57300183-57300202 Msa0859120:CDS 35.0%
!! GGGAAAGAGTTGATGAAATT+GGG + chr5_4:57300184-57300203 Msa0859120:CDS 35.0%
TGAAATTGGGATCGTTGAAA+TGG + chr5_4:57300197-57300216 Msa0859120:CDS 35.0%
CTTGTAGATCTCAATGATCA+AGG + chr5_4:57300588-57300607 Msa0859120:three_prime_UTR 35.0%
AAAACAAGATAAGTGGGTGA+TGG - chr5_4:57299663-57299682 None:intergenic 35.0%
ACCAACAACAAACACATCAA+TGG + chr5_4:57299690-57299709 Msa0859120:exon 35.0%
!! TCCATTGATGTGTTTGTTGT+TGG - chr5_4:57299694-57299713 None:intergenic 35.0%
!! TGGGAAAGAGTTGATGAAAT+TGG + chr5_4:57300183-57300202 Msa0859120:CDS 35.0%
!! GGGAAAGAGTTGATGAAATT+GGG + chr5_4:57300184-57300203 Msa0859120:CDS 35.0%
TGAAATTGGGATCGTTGAAA+TGG + chr5_4:57300197-57300216 Msa0859120:CDS 35.0%
CTTGTAGATCTCAATGATCA+AGG + chr5_4:57300588-57300607 Msa0859120:three_prime_UTR 35.0%
!! TTGGATAGTTCTGAGGATCT+TGG - chr5_4:57299876-57299895 None:intergenic 40.0%
! GAGTCATTTGGATAGTTCTG+AGG - chr5_4:57299883-57299902 None:intergenic 40.0%
GGGGTTCAATTTCATGAACA+TGG - chr5_4:57299945-57299964 None:intergenic 40.0%
TTGAAATGGAGAGGATCTGA+TGG + chr5_4:57300211-57300230 Msa0859120:CDS 40.0%
!! TTGGATAGTTCTGAGGATCT+TGG - chr5_4:57299876-57299895 None:intergenic 40.0%
! GAGTCATTTGGATAGTTCTG+AGG - chr5_4:57299883-57299902 None:intergenic 40.0%
GGGGTTCAATTTCATGAACA+TGG - chr5_4:57299945-57299964 None:intergenic 40.0%
TTGAAATGGAGAGGATCTGA+TGG + chr5_4:57300211-57300230 Msa0859120:CDS 40.0%
TAAGCTAGTGGGACGAACAT+TGG - chr5_4:57299766-57299785 None:intergenic 45.0%
CCACAGTACGATAAGCTAGT+GGG - chr5_4:57299777-57299796 None:intergenic 45.0%
TCCACAGTACGATAAGCTAG+TGG - chr5_4:57299778-57299797 None:intergenic 45.0%
! ACTAGCTTATCGTACTGTGG+AGG + chr5_4:57299777-57299796 Msa0859120:CDS 45.0%
TGGAGGATTTCTCCGATGAA+TGG + chr5_4:57299794-57299813 Msa0859120:CDS 45.0%
AGGATTTCTCCGATGAATGG+TGG + chr5_4:57299797-57299816 Msa0859120:CDS 45.0%
AACGAGGTTCCACCATTCAT+CGG - chr5_4:57299809-57299828 None:intergenic 45.0%
TGCGTGTAGAACACGAAGAT+GGG + chr5_4:57300164-57300183 Msa0859120:intron 45.0%
TTGGGATCGTTGAAATGGAG+AGG + chr5_4:57300202-57300221 Msa0859120:CDS 45.0%
AATGGAGAGGATCTGATGGA+TGG + chr5_4:57300215-57300234 Msa0859120:CDS 45.0%
! ATGGAGAGGATCTGATGGAT+GGG + chr5_4:57300216-57300235 Msa0859120:CDS 45.0%
AGTTCCAAGGTATGCTGAGA+AGG + chr5_4:57300243-57300262 Msa0859120:CDS 45.0%
ACTCTGGGCTTCACAATCTT+CGG - chr5_4:57300271-57300290 None:intergenic 45.0%
TTCATCAGCCCAGGTAGATT+TGG + chr5_4:57300305-57300324 Msa0859120:exon 45.0%
CTTTGCCACCAAATCTACCT+GGG - chr5_4:57300316-57300335 None:intergenic 45.0%
ACTTTGCCACCAAATCTACC+TGG - chr5_4:57300317-57300336 None:intergenic 45.0%
!! AGGTAGATTTGGTGGCAAAG+TGG + chr5_4:57300316-57300335 Msa0859120:exon 45.0%
! TTTGGTGGCAAAGTGGAAAC+TGG + chr5_4:57300323-57300342 Msa0859120:three_prime_UTR 45.0%
TAAGCTAGTGGGACGAACAT+TGG - chr5_4:57299766-57299785 None:intergenic 45.0%
CCACAGTACGATAAGCTAGT+GGG - chr5_4:57299777-57299796 None:intergenic 45.0%
TCCACAGTACGATAAGCTAG+TGG - chr5_4:57299778-57299797 None:intergenic 45.0%
! ACTAGCTTATCGTACTGTGG+AGG + chr5_4:57299777-57299796 Msa0859120:CDS 45.0%
TGGAGGATTTCTCCGATGAA+TGG + chr5_4:57299794-57299813 Msa0859120:CDS 45.0%
AGGATTTCTCCGATGAATGG+TGG + chr5_4:57299797-57299816 Msa0859120:CDS 45.0%
AACGAGGTTCCACCATTCAT+CGG - chr5_4:57299809-57299828 None:intergenic 45.0%
TGCGTGTAGAACACGAAGAT+GGG + chr5_4:57300164-57300183 Msa0859120:intron 45.0%
TTGGGATCGTTGAAATGGAG+AGG + chr5_4:57300202-57300221 Msa0859120:CDS 45.0%
AATGGAGAGGATCTGATGGA+TGG + chr5_4:57300215-57300234 Msa0859120:CDS 45.0%
! ATGGAGAGGATCTGATGGAT+GGG + chr5_4:57300216-57300235 Msa0859120:CDS 45.0%
AGTTCCAAGGTATGCTGAGA+AGG + chr5_4:57300243-57300262 Msa0859120:CDS 45.0%
ACTCTGGGCTTCACAATCTT+CGG - chr5_4:57300271-57300290 None:intergenic 45.0%
TTCATCAGCCCAGGTAGATT+TGG + chr5_4:57300305-57300324 Msa0859120:exon 45.0%
CTTTGCCACCAAATCTACCT+GGG - chr5_4:57300316-57300335 None:intergenic 45.0%
ACTTTGCCACCAAATCTACC+TGG - chr5_4:57300317-57300336 None:intergenic 45.0%
!! AGGTAGATTTGGTGGCAAAG+TGG + chr5_4:57300316-57300335 Msa0859120:exon 45.0%
! TTTGGTGGCAAAGTGGAAAC+TGG + chr5_4:57300323-57300342 Msa0859120:three_prime_UTR 45.0%
GTTGGGATTCAACGAGGAAG+AGG - chr5_4:57299740-57299759 None:intergenic 50.0%
! CCCACTAGCTTATCGTACTG+TGG + chr5_4:57299774-57299793 Msa0859120:CDS 50.0%
CCAGTAATCACGGAACCAAG+GGG - chr5_4:57299839-57299858 None:intergenic 50.0%
GCCAGTAATCACGGAACCAA+GGG - chr5_4:57299840-57299859 None:intergenic 50.0%
AGCCAGTAATCACGGAACCA+AGG - chr5_4:57299841-57299860 None:intergenic 50.0%
GTTCACGGAGCCAGTAATCA+CGG - chr5_4:57299849-57299868 None:intergenic 50.0%
!! GAGGATCTTGGAAGCGTTCA+CGG - chr5_4:57299864-57299883 None:intergenic 50.0%
GTGCGTGTAGAACACGAAGA+TGG + chr5_4:57300163-57300182 Msa0859120:intron 50.0%
!! ATGGATGGGCTGAAGTTCCA+AGG + chr5_4:57300230-57300249 Msa0859120:CDS 50.0%
ATCAGCCCAGGTAGATTTGG+TGG + chr5_4:57300308-57300327 Msa0859120:exon 50.0%
GTTGGGATTCAACGAGGAAG+AGG - chr5_4:57299740-57299759 None:intergenic 50.0%
! CCCACTAGCTTATCGTACTG+TGG + chr5_4:57299774-57299793 Msa0859120:CDS 50.0%
CCAGTAATCACGGAACCAAG+GGG - chr5_4:57299839-57299858 None:intergenic 50.0%
GCCAGTAATCACGGAACCAA+GGG - chr5_4:57299840-57299859 None:intergenic 50.0%
AGCCAGTAATCACGGAACCA+AGG - chr5_4:57299841-57299860 None:intergenic 50.0%
GTTCACGGAGCCAGTAATCA+CGG - chr5_4:57299849-57299868 None:intergenic 50.0%
!! GAGGATCTTGGAAGCGTTCA+CGG - chr5_4:57299864-57299883 None:intergenic 50.0%
GTGCGTGTAGAACACGAAGA+TGG + chr5_4:57300163-57300182 Msa0859120:intron 50.0%
!! ATGGATGGGCTGAAGTTCCA+AGG + chr5_4:57300230-57300249 Msa0859120:CDS 50.0%
ATCAGCCCAGGTAGATTTGG+TGG + chr5_4:57300308-57300327 Msa0859120:exon 50.0%
!! TGGAGCGTTGGGATTCAACG+AGG - chr5_4:57299746-57299765 None:intergenic 55.0%
! GGGACGAACATTGGAGCGTT+GGG - chr5_4:57299757-57299776 None:intergenic 55.0%
TGGGACGAACATTGGAGCGT+TGG - chr5_4:57299758-57299777 None:intergenic 55.0%
ACCTCGTTCATTCCTCCCCT+TGG + chr5_4:57299821-57299840 Msa0859120:CDS 55.0%
ACCAAGGGGAGGAATGAACG+AGG - chr5_4:57299825-57299844 None:intergenic 55.0%
GTAATCACGGAACCAAGGGG+AGG - chr5_4:57299836-57299855 None:intergenic 55.0%
CCCCTTGGTTCCGTGATTAC+TGG + chr5_4:57299836-57299855 Msa0859120:CDS 55.0%
GGAGCCTTCTCAGCATACCT+TGG - chr5_4:57300250-57300269 None:intergenic 55.0%
TGGGCTGATGAATAGCCCGT+GGG - chr5_4:57300297-57300316 None:intergenic 55.0%
!! TGGAGCGTTGGGATTCAACG+AGG - chr5_4:57299746-57299765 None:intergenic 55.0%
! GGGACGAACATTGGAGCGTT+GGG - chr5_4:57299757-57299776 None:intergenic 55.0%
TGGGACGAACATTGGAGCGT+TGG - chr5_4:57299758-57299777 None:intergenic 55.0%
ACCTCGTTCATTCCTCCCCT+TGG + chr5_4:57299821-57299840 Msa0859120:CDS 55.0%
ACCAAGGGGAGGAATGAACG+AGG - chr5_4:57299825-57299844 None:intergenic 55.0%
GTAATCACGGAACCAAGGGG+AGG - chr5_4:57299836-57299855 None:intergenic 55.0%
CCCCTTGGTTCCGTGATTAC+TGG + chr5_4:57299836-57299855 Msa0859120:CDS 55.0%
GGAGCCTTCTCAGCATACCT+TGG - chr5_4:57300250-57300269 None:intergenic 55.0%
TGGGCTGATGAATAGCCCGT+GGG - chr5_4:57300297-57300316 None:intergenic 55.0%
TGAAGCCCAGAGTGAGCCCA+CGG + chr5_4:57300278-57300297 Msa0859120:CDS 60.0%
ATAGCCCGTGGGCTCACTCT+GGG - chr5_4:57300286-57300305 None:intergenic 60.0%
AATAGCCCGTGGGCTCACTC+TGG - chr5_4:57300287-57300306 None:intergenic 60.0%
CTGGGCTGATGAATAGCCCG+TGG - chr5_4:57300298-57300317 None:intergenic 60.0%
! CACGGGCTATTCATCAGCCC+AGG + chr5_4:57300296-57300315 Msa0859120:CDS 60.0%
TGAAGCCCAGAGTGAGCCCA+CGG + chr5_4:57300278-57300297 Msa0859120:CDS 60.0%
ATAGCCCGTGGGCTCACTCT+GGG - chr5_4:57300286-57300305 None:intergenic 60.0%
AATAGCCCGTGGGCTCACTC+TGG - chr5_4:57300287-57300306 None:intergenic 60.0%
CTGGGCTGATGAATAGCCCG+TGG - chr5_4:57300298-57300317 None:intergenic 60.0%
! CACGGGCTATTCATCAGCCC+AGG + chr5_4:57300296-57300315 Msa0859120:CDS 60.0%
GAAGCCCAGAGTGAGCCCAC+GGG + chr5_4:57300279-57300298 Msa0859120:CDS 65.0%
GAAGCCCAGAGTGAGCCCAC+GGG + chr5_4:57300279-57300298 Msa0859120:CDS 65.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 57299617 57300646 57299617 ID=Msa0859120;Name=Msa0859120
chr5_4 mRNA 57299617 57300646 57299617 ID=Msa0859120-mRNA-1;Parent=Msa0859120;Name=Msa0859120-mRNA-1;_AED=0.02;_eAED=0.02;_QI=92|1|1|1|1|1|2|325|134
chr5_4 exon 57299617 57299964 57299617 ID=Msa0859120-mRNA-1:exon:17466;Parent=Msa0859120-mRNA-1
chr5_4 exon 57300173 57300646 57300173 ID=Msa0859120-mRNA-1:exon:17467;Parent=Msa0859120-mRNA-1
chr5_4 five_prime_UTR 57299617 57299708 57299617 ID=Msa0859120-mRNA-1:five_prime_utr;Parent=Msa0859120-mRNA-1
chr5_4 CDS 57299709 57299964 57299709 ID=Msa0859120-mRNA-1:cds;Parent=Msa0859120-mRNA-1
chr5_4 CDS 57300173 57300321 57300173 ID=Msa0859120-mRNA-1:cds;Parent=Msa0859120-mRNA-1
chr5_4 three_prime_UTR 57300322 57300646 57300322 ID=Msa0859120-mRNA-1:three_prime_utr;Parent=Msa0859120-mRNA-1
Gene Sequence

>Msa0859120

ATGGAAGTTCTTTCTGCTACAAGAACATCCTCTTCCTCGTTGAATCCCAACGCTCCAATGTTCGTCCCACTAGCTTATCGTACTGTGGAGGATTTCTCCGATGAATGGTGGAACCTCGTTCATTCCTCCCCTTGGTTCCGTGATTACTGGCTCCGTGAACGCTTCCAAGATCCTCAGAACTATCCAAATGACTCGTTTTCTGATTTTGAAATGATGGATGAAGATGATCTTTTCCATGTTCATGAAATTGAACCCCAACACGAAGATGGGAAAGAGTTGATGAAATTGGGATCGTTGAAATGGAGAGGATCTGATGGATGGGCTGAAGTTCCAAGGTATGCTGAGAAGGCTCCGAAGATTGTGAAGCCCAGAGTGAGCCCACGGGCTATTCATCAGCCCAGGTAG
Protein sequence

>Msa0859120

MEVLSATRTSSSSLNPNAPMFVPLAYRTVEDFSDEWWNLVHSSPWFRDYWLRERFQDPQNYPNDSFSDFEMMDEDDLFHVHEIEPQHEDGKELMKLGSLKWRGSDGWAEVPRYAEKAPKIVKPRVSPRAIHQPR*