AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0783680

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0783680 MtrunA17_Chr5g0437861 99.254 134 1 0 1 134 1 134 4.37e-98 277
Msa0783680 MtrunA17_Chr3g0085831 32.544 169 76 3 4 134 1 169 4.14e-21 83.6
Msa0783680 MtrunA17_Chr7g0256851 44.595 74 37 2 1 73 1 71 1.67e-15 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0783680 AT2G41430.5 31.176 170 74 5 1 134 1 163 6.71e-15 67.8
Msa0783680 AT2G41430.4 31.176 170 74 5 1 134 1 163 6.71e-15 67.8
Msa0783680 AT2G41430.2 31.176 170 74 5 1 134 1 163 6.71e-15 67.8
Msa0783680 AT2G41430.1 31.176 170 74 5 1 134 1 163 6.71e-15 67.8
Msa0783680 AT2G41430.3 44.615 65 33 1 1 65 1 62 3.08e-14 64.7
Msa0783680 AT4G14270.1 48.485 66 26 2 12 75 10 69 4.56e-14 65.1
Msa0783680 AT4G14270.2 48.485 66 26 2 12 75 10 69 4.56e-14 65.1

Find 55 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGAGTTATGATTTGATTTC+AGG 0.153500 5_2:-67829777 Msa0783680:three_prime_UTR
CCCCTTGGTTCCGTGATTAC+TGG 0.164334 5_2:-67830350 Msa0783680:CDS
TGGGAAACAGTTGATGAAAT+TGG 0.209998 5_2:-67829989 Msa0783680:CDS
TTCATCAGCCCAGGTAGATT+TGG 0.348718 5_2:-67829867 Msa0783680:exon
ACCTCGTTCATTCCTCCCCT+TGG 0.371694 5_2:-67830365 Msa0783680:CDS
TTTGGTGGCAAAGTGGAAAC+TGG 0.402698 5_2:-67829849 Msa0783680:three_prime_UTR
TTTGATAGTTCTGAGGATCT+TGG 0.417299 5_2:+67830313 None:intergenic
TCAAGGTTGAAAATTTGTTA+AGG 0.423651 5_2:-67829563 Msa0783680:three_prime_UTR
GAGGATCTTGGAAGCGTTCA+CGG 0.427509 5_2:+67830325 None:intergenic
GGGAAACAGTTGATGAAATT+GGG 0.429131 5_2:-67829988 Msa0783680:CDS
ACTCTGGGTTTCACAATCTT+CGG 0.430263 5_2:+67829904 None:intergenic
AGAGTTATGATTTGATTTCA+GGG 0.438755 5_2:-67829776 Msa0783680:three_prime_UTR
ACTTCCATAGCTGCGTTTGT+TGG 0.442292 5_2:+67830492 None:intergenic
TAAGTTGTTGTAATGTTACT+TGG 0.443682 5_2:-67829629 Msa0783680:three_prime_UTR
TGAAATTGGGATCGTTGAAA+TGG 0.456391 5_2:-67829975 Msa0783680:CDS
GCGCGTGTAGAACACGAAGA+TGG 0.465797 5_2:-67830009 Msa0783680:intron
CGCGTGTAGAACACGAAGAT+GGG 0.474343 5_2:-67830008 Msa0783680:intron
GGAACGAACATCGGAGCGTT+GGG 0.476212 5_2:+67830432 None:intergenic
CGGAACGAACATCGGAGCGT+TGG 0.492883 5_2:+67830431 None:intergenic
ATCAGCCCAGGTAGATTTGG+TGG 0.507054 5_2:-67829864 Msa0783680:exon
CTTGTAGATCTCAATGATCA+AGG 0.510028 5_2:-67829580 Msa0783680:three_prime_UTR
ACTTTGCCACCAAATCTACC+TGG 0.520178 5_2:+67829858 None:intergenic
GGGGTTCAATTTCATGAACA+TGG 0.523834 5_2:+67830244 None:intergenic
GGAGCCTTCTCAGCATACCT+TGG 0.524540 5_2:+67829925 None:intergenic
GTTGGGATTCAGCGAGGAGG+AGG 0.529767 5_2:+67830449 None:intergenic
TGGAGGATTTCTCCGATGAA+TGG 0.530316 5_2:-67830392 Msa0783680:CDS
ATCACCAACAAACGCAGCTA+TGG 0.534245 5_2:-67830496 Msa0783680:exon
ATAGCCCGTGGGCTCACTCT+GGG 0.536141 5_2:+67829889 None:intergenic
GCCAGTAATCACGGAACCAA+GGG 0.550209 5_2:+67830349 None:intergenic
AGTTCCAAGGTATGCTGAGA+AGG 0.558393 5_2:-67829929 Msa0783680:CDS
AAAAGAAGATAAGAATGAAG+CGG 0.560581 5_2:+67830538 None:intergenic
AACGAGGTTCCACCATTCAT+CGG 0.566948 5_2:+67830380 None:intergenic
TTGAAATGGAGAGGATCTGA+TGG 0.578122 5_2:-67829961 Msa0783680:CDS
CACGGGCTATTCATCAGCCC+AGG 0.580678 5_2:-67829876 Msa0783680:CDS
TACGCAAGCGGAACGAACAT+CGG 0.582960 5_2:+67830423 None:intergenic
ATGGATGGGCTGAAGTTCCA+AGG 0.594708 5_2:-67829942 Msa0783680:CDS
GCTTGCGTATCGTACGGTGG+AGG 0.600361 5_2:-67830409 Msa0783680:CDS
TTGGGATCGTTGAAATGGAG+AGG 0.600872 5_2:-67829970 Msa0783680:CDS
ATGTTACTTGGAAAATTGTG+TGG 0.602757 5_2:-67829617 Msa0783680:three_prime_UTR
AGGTAGATTTGGTGGCAAAG+TGG 0.603013 5_2:-67829856 Msa0783680:exon
TGAAACCCAGAGTGAGCCCA+CGG 0.604003 5_2:-67829894 Msa0783680:CDS
AGCCAGTAATCACGGAACCA+AGG 0.611593 5_2:+67830348 None:intergenic
AGGATTTCTCCGATGAATGG+TGG 0.617511 5_2:-67830389 Msa0783680:CDS
AATGGAGAGGATCTGATGGA+TGG 0.618481 5_2:-67829957 Msa0783680:CDS
ATGGAGAGGATCTGATGGAT+GGG 0.622633 5_2:-67829956 Msa0783680:CDS
CTTTGCCACCAAATCTACCT+GGG 0.625860 5_2:+67829859 None:intergenic
GTTCACGGAGCCAGTAATCA+CGG 0.629661 5_2:+67830340 None:intergenic
TGTTACTTGGAAAATTGTGT+GGG 0.652575 5_2:-67829616 Msa0783680:three_prime_UTR
GGCAAAGTGGAAACTGGAAA+CGG 0.655877 5_2:-67829843 Msa0783680:three_prime_UTR
GAAACCCAGAGTGAGCCCAC+GGG 0.661476 5_2:-67829893 Msa0783680:CDS
TCCGCTTGCGTATCGTACGG+TGG 0.666184 5_2:-67830412 Msa0783680:CDS
AGCGTTGGGATTCAGCGAGG+AGG 0.680838 5_2:+67830446 None:intergenic
GTAATCACGGAACCAAGGGG+AGG 0.688535 5_2:+67830353 None:intergenic
CCAGTAATCACGGAACCAAG+GGG 0.718986 5_2:+67830350 None:intergenic
ACCAAGGGGAGGAATGAACG+AGG 0.740764 5_2:+67830364 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAACTCTAGTTATTTTTTTA+CGG + chr5_2:67830325-67830344 None:intergenic 15.0%
!!! TAACTCTAGTTATTTTTTTA+CGG + chr5_2:67830325-67830344 None:intergenic 15.0%
!! AATAAAGTAAAATAGCTTAC+GGG + chr5_2:67829893-67829912 None:intergenic 20.0%
!! CAATAAAGTAAAATAGCTTA+CGG + chr5_2:67829894-67829913 None:intergenic 20.0%
!!! AACTCTAGTTATTTTTTTAC+GGG + chr5_2:67830324-67830343 None:intergenic 20.0%
!! AATAAAGTAAAATAGCTTAC+GGG + chr5_2:67829893-67829912 None:intergenic 20.0%
!! CAATAAAGTAAAATAGCTTA+CGG + chr5_2:67829894-67829913 None:intergenic 20.0%
!!! AACTCTAGTTATTTTTTTAC+GGG + chr5_2:67830324-67830343 None:intergenic 20.0%
! AAAAGAAGATAAGAATGAAG+CGG + chr5_2:67829579-67829598 None:intergenic 25.0%
!!! GTTTTCTGATTTTGAAATGA+TGG - chr5_2:67829831-67829850 Msa0783680:three_prime_UTR 25.0%
! ATAAAGTAAAATAGCTTACG+GGG + chr5_2:67829892-67829911 None:intergenic 25.0%
! CATTAACTCATCAAGATTAA+TGG + chr5_2:67830068-67830087 None:intergenic 25.0%
! CATTAATCTTGATGAGTTAA+TGG - chr5_2:67830066-67830085 Msa0783680:intron 25.0%
!! TAGAGTTATGATTTGATTTC+AGG - chr5_2:67830337-67830356 Msa0783680:CDS 25.0%
!! AGAGTTATGATTTGATTTCA+GGG - chr5_2:67830338-67830357 Msa0783680:CDS 25.0%
! TAAGTTGTTGTAATGTTACT+TGG - chr5_2:67830485-67830504 Msa0783680:exon 25.0%
!!! TCAAGGTTGAAAATTTGTTA+AGG - chr5_2:67830551-67830570 Msa0783680:five_prime_UTR 25.0%
! AAAAGAAGATAAGAATGAAG+CGG + chr5_2:67829579-67829598 None:intergenic 25.0%
!!! GTTTTCTGATTTTGAAATGA+TGG - chr5_2:67829831-67829850 Msa0783680:three_prime_UTR 25.0%
! ATAAAGTAAAATAGCTTACG+GGG + chr5_2:67829892-67829911 None:intergenic 25.0%
! CATTAACTCATCAAGATTAA+TGG + chr5_2:67830068-67830087 None:intergenic 25.0%
! CATTAATCTTGATGAGTTAA+TGG - chr5_2:67830066-67830085 Msa0783680:intron 25.0%
!! TAGAGTTATGATTTGATTTC+AGG - chr5_2:67830337-67830356 Msa0783680:CDS 25.0%
!! AGAGTTATGATTTGATTTCA+GGG - chr5_2:67830338-67830357 Msa0783680:CDS 25.0%
! TAAGTTGTTGTAATGTTACT+TGG - chr5_2:67830485-67830504 Msa0783680:exon 25.0%
!!! TCAAGGTTGAAAATTTGTTA+AGG - chr5_2:67830551-67830570 Msa0783680:five_prime_UTR 25.0%
!!! TAATGGACTGATGTTTGTTT+TGG - chr5_2:67830083-67830102 Msa0783680:intron 30.0%
! ATGTTACTTGGAAAATTGTG+TGG - chr5_2:67830497-67830516 Msa0783680:exon 30.0%
! TGTTACTTGGAAAATTGTGT+GGG - chr5_2:67830498-67830517 Msa0783680:exon 30.0%
!!! TAATGGACTGATGTTTGTTT+TGG - chr5_2:67830083-67830102 Msa0783680:intron 30.0%
! ATGTTACTTGGAAAATTGTG+TGG - chr5_2:67830497-67830516 Msa0783680:exon 30.0%
! TGTTACTTGGAAAATTGTGT+GGG - chr5_2:67830498-67830517 Msa0783680:exon 30.0%
!! TTTGATAGTTCTGAGGATCT+TGG + chr5_2:67829804-67829823 None:intergenic 35.0%
!! GAGTCATTTTGATAGTTCTG+AGG + chr5_2:67829811-67829830 None:intergenic 35.0%
!! GACGTAGCAAAAGTATGTTT+TGG - chr5_2:67830022-67830041 Msa0783680:intron 35.0%
!! TGGGAAACAGTTGATGAAAT+TGG - chr5_2:67830125-67830144 Msa0783680:intron 35.0%
!! GGGAAACAGTTGATGAAATT+GGG - chr5_2:67830126-67830145 Msa0783680:intron 35.0%
TGAAATTGGGATCGTTGAAA+TGG - chr5_2:67830139-67830158 Msa0783680:intron 35.0%
CTTGTAGATCTCAATGATCA+AGG - chr5_2:67830534-67830553 Msa0783680:five_prime_UTR 35.0%
!! TTTGATAGTTCTGAGGATCT+TGG + chr5_2:67829804-67829823 None:intergenic 35.0%
!! GAGTCATTTTGATAGTTCTG+AGG + chr5_2:67829811-67829830 None:intergenic 35.0%
!! GACGTAGCAAAAGTATGTTT+TGG - chr5_2:67830022-67830041 Msa0783680:intron 35.0%
!! TGGGAAACAGTTGATGAAAT+TGG - chr5_2:67830125-67830144 Msa0783680:intron 35.0%
!! GGGAAACAGTTGATGAAATT+GGG - chr5_2:67830126-67830145 Msa0783680:intron 35.0%
TGAAATTGGGATCGTTGAAA+TGG - chr5_2:67830139-67830158 Msa0783680:intron 35.0%
CTTGTAGATCTCAATGATCA+AGG - chr5_2:67830534-67830553 Msa0783680:five_prime_UTR 35.0%
GGGGTTCAATTTCATGAACA+TGG + chr5_2:67829873-67829892 None:intergenic 40.0%
TTGAAATGGAGAGGATCTGA+TGG - chr5_2:67830153-67830172 Msa0783680:intron 40.0%
ACTCTGGGTTTCACAATCTT+CGG + chr5_2:67830213-67830232 None:intergenic 40.0%
GGGGTTCAATTTCATGAACA+TGG + chr5_2:67829873-67829892 None:intergenic 40.0%
TTGAAATGGAGAGGATCTGA+TGG - chr5_2:67830153-67830172 Msa0783680:intron 40.0%
ACTCTGGGTTTCACAATCTT+CGG + chr5_2:67830213-67830232 None:intergenic 40.0%
ATCACCAACAAACGCAGCTA+TGG - chr5_2:67829618-67829637 Msa0783680:three_prime_UTR 45.0%
ACTTCCATAGCTGCGTTTGT+TGG + chr5_2:67829625-67829644 None:intergenic 45.0%
TGGAGGATTTCTCCGATGAA+TGG - chr5_2:67829722-67829741 Msa0783680:three_prime_UTR 45.0%
AGGATTTCTCCGATGAATGG+TGG - chr5_2:67829725-67829744 Msa0783680:three_prime_UTR 45.0%
AACGAGGTTCCACCATTCAT+CGG + chr5_2:67829737-67829756 None:intergenic 45.0%
TTGGGATCGTTGAAATGGAG+AGG - chr5_2:67830144-67830163 Msa0783680:intron 45.0%
AATGGAGAGGATCTGATGGA+TGG - chr5_2:67830157-67830176 Msa0783680:intron 45.0%
! ATGGAGAGGATCTGATGGAT+GGG - chr5_2:67830158-67830177 Msa0783680:intron 45.0%
AGTTCCAAGGTATGCTGAGA+AGG - chr5_2:67830185-67830204 Msa0783680:intron 45.0%
TTCATCAGCCCAGGTAGATT+TGG - chr5_2:67830247-67830266 Msa0783680:CDS 45.0%
CTTTGCCACCAAATCTACCT+GGG + chr5_2:67830258-67830277 None:intergenic 45.0%
ACTTTGCCACCAAATCTACC+TGG + chr5_2:67830259-67830278 None:intergenic 45.0%
!! AGGTAGATTTGGTGGCAAAG+TGG - chr5_2:67830258-67830277 Msa0783680:CDS 45.0%
! TTTGGTGGCAAAGTGGAAAC+TGG - chr5_2:67830265-67830284 Msa0783680:CDS 45.0%
GGCAAAGTGGAAACTGGAAA+CGG - chr5_2:67830271-67830290 Msa0783680:CDS 45.0%
ATCACCAACAAACGCAGCTA+TGG - chr5_2:67829618-67829637 Msa0783680:three_prime_UTR 45.0%
ACTTCCATAGCTGCGTTTGT+TGG + chr5_2:67829625-67829644 None:intergenic 45.0%
TGGAGGATTTCTCCGATGAA+TGG - chr5_2:67829722-67829741 Msa0783680:three_prime_UTR 45.0%
AGGATTTCTCCGATGAATGG+TGG - chr5_2:67829725-67829744 Msa0783680:three_prime_UTR 45.0%
AACGAGGTTCCACCATTCAT+CGG + chr5_2:67829737-67829756 None:intergenic 45.0%
TTGGGATCGTTGAAATGGAG+AGG - chr5_2:67830144-67830163 Msa0783680:intron 45.0%
AATGGAGAGGATCTGATGGA+TGG - chr5_2:67830157-67830176 Msa0783680:intron 45.0%
! ATGGAGAGGATCTGATGGAT+GGG - chr5_2:67830158-67830177 Msa0783680:intron 45.0%
AGTTCCAAGGTATGCTGAGA+AGG - chr5_2:67830185-67830204 Msa0783680:intron 45.0%
TTCATCAGCCCAGGTAGATT+TGG - chr5_2:67830247-67830266 Msa0783680:CDS 45.0%
CTTTGCCACCAAATCTACCT+GGG + chr5_2:67830258-67830277 None:intergenic 45.0%
ACTTTGCCACCAAATCTACC+TGG + chr5_2:67830259-67830278 None:intergenic 45.0%
!! AGGTAGATTTGGTGGCAAAG+TGG - chr5_2:67830258-67830277 Msa0783680:CDS 45.0%
! TTTGGTGGCAAAGTGGAAAC+TGG - chr5_2:67830265-67830284 Msa0783680:CDS 45.0%
GGCAAAGTGGAAACTGGAAA+CGG - chr5_2:67830271-67830290 Msa0783680:CDS 45.0%
TACGCAAGCGGAACGAACAT+CGG + chr5_2:67829694-67829713 None:intergenic 50.0%
CCAGTAATCACGGAACCAAG+GGG + chr5_2:67829767-67829786 None:intergenic 50.0%
GCCAGTAATCACGGAACCAA+GGG + chr5_2:67829768-67829787 None:intergenic 50.0%
AGCCAGTAATCACGGAACCA+AGG + chr5_2:67829769-67829788 None:intergenic 50.0%
GTTCACGGAGCCAGTAATCA+CGG + chr5_2:67829777-67829796 None:intergenic 50.0%
!! GAGGATCTTGGAAGCGTTCA+CGG + chr5_2:67829792-67829811 None:intergenic 50.0%
CGCGTGTAGAACACGAAGAT+GGG - chr5_2:67830106-67830125 Msa0783680:intron 50.0%
!! ATGGATGGGCTGAAGTTCCA+AGG - chr5_2:67830172-67830191 Msa0783680:intron 50.0%
ATCAGCCCAGGTAGATTTGG+TGG - chr5_2:67830250-67830269 Msa0783680:CDS 50.0%
TACGCAAGCGGAACGAACAT+CGG + chr5_2:67829694-67829713 None:intergenic 50.0%
CCAGTAATCACGGAACCAAG+GGG + chr5_2:67829767-67829786 None:intergenic 50.0%
GCCAGTAATCACGGAACCAA+GGG + chr5_2:67829768-67829787 None:intergenic 50.0%
AGCCAGTAATCACGGAACCA+AGG + chr5_2:67829769-67829788 None:intergenic 50.0%
GTTCACGGAGCCAGTAATCA+CGG + chr5_2:67829777-67829796 None:intergenic 50.0%
!! GAGGATCTTGGAAGCGTTCA+CGG + chr5_2:67829792-67829811 None:intergenic 50.0%
CGCGTGTAGAACACGAAGAT+GGG - chr5_2:67830106-67830125 Msa0783680:intron 50.0%
!! ATGGATGGGCTGAAGTTCCA+AGG - chr5_2:67830172-67830191 Msa0783680:intron 50.0%
ATCAGCCCAGGTAGATTTGG+TGG - chr5_2:67830250-67830269 Msa0783680:CDS 50.0%
! GGAACGAACATCGGAGCGTT+GGG + chr5_2:67829685-67829704 None:intergenic 55.0%
CGTTCCGCTTGCGTATCGTA+CGG - chr5_2:67829699-67829718 Msa0783680:three_prime_UTR 55.0%
TCCACCGTACGATACGCAAG+CGG + chr5_2:67829706-67829725 None:intergenic 55.0%
ACCTCGTTCATTCCTCCCCT+TGG - chr5_2:67829749-67829768 Msa0783680:three_prime_UTR 55.0%
ACCAAGGGGAGGAATGAACG+AGG + chr5_2:67829753-67829772 None:intergenic 55.0%
GTAATCACGGAACCAAGGGG+AGG + chr5_2:67829764-67829783 None:intergenic 55.0%
CCCCTTGGTTCCGTGATTAC+TGG - chr5_2:67829764-67829783 Msa0783680:three_prime_UTR 55.0%
GCGCGTGTAGAACACGAAGA+TGG - chr5_2:67830105-67830124 Msa0783680:intron 55.0%
GGAGCCTTCTCAGCATACCT+TGG + chr5_2:67830192-67830211 None:intergenic 55.0%
TGAAACCCAGAGTGAGCCCA+CGG - chr5_2:67830220-67830239 Msa0783680:intron 55.0%
TGGGCTGATGAATAGCCCGT+GGG + chr5_2:67830239-67830258 None:intergenic 55.0%
! GGAACGAACATCGGAGCGTT+GGG + chr5_2:67829685-67829704 None:intergenic 55.0%
CGTTCCGCTTGCGTATCGTA+CGG - chr5_2:67829699-67829718 Msa0783680:three_prime_UTR 55.0%
TCCACCGTACGATACGCAAG+CGG + chr5_2:67829706-67829725 None:intergenic 55.0%
ACCTCGTTCATTCCTCCCCT+TGG - chr5_2:67829749-67829768 Msa0783680:three_prime_UTR 55.0%
ACCAAGGGGAGGAATGAACG+AGG + chr5_2:67829753-67829772 None:intergenic 55.0%
GTAATCACGGAACCAAGGGG+AGG + chr5_2:67829764-67829783 None:intergenic 55.0%
CCCCTTGGTTCCGTGATTAC+TGG - chr5_2:67829764-67829783 Msa0783680:three_prime_UTR 55.0%
GCGCGTGTAGAACACGAAGA+TGG - chr5_2:67830105-67830124 Msa0783680:intron 55.0%
GGAGCCTTCTCAGCATACCT+TGG + chr5_2:67830192-67830211 None:intergenic 55.0%
TGAAACCCAGAGTGAGCCCA+CGG - chr5_2:67830220-67830239 Msa0783680:intron 55.0%
TGGGCTGATGAATAGCCCGT+GGG + chr5_2:67830239-67830258 None:intergenic 55.0%
GTTGGGATTCAGCGAGGAGG+AGG + chr5_2:67829668-67829687 None:intergenic 60.0%
! AGCGTTGGGATTCAGCGAGG+AGG + chr5_2:67829671-67829690 None:intergenic 60.0%
CGGAACGAACATCGGAGCGT+TGG + chr5_2:67829686-67829705 None:intergenic 60.0%
TCCGCTTGCGTATCGTACGG+TGG - chr5_2:67829702-67829721 Msa0783680:three_prime_UTR 60.0%
GCTTGCGTATCGTACGGTGG+AGG - chr5_2:67829705-67829724 Msa0783680:three_prime_UTR 60.0%
GAAACCCAGAGTGAGCCCAC+GGG - chr5_2:67830221-67830240 Msa0783680:intron 60.0%
ATAGCCCGTGGGCTCACTCT+GGG + chr5_2:67830228-67830247 None:intergenic 60.0%
AATAGCCCGTGGGCTCACTC+TGG + chr5_2:67830229-67830248 None:intergenic 60.0%
CTGGGCTGATGAATAGCCCG+TGG + chr5_2:67830240-67830259 None:intergenic 60.0%
! CACGGGCTATTCATCAGCCC+AGG - chr5_2:67830238-67830257 Msa0783680:intron 60.0%
GTTGGGATTCAGCGAGGAGG+AGG + chr5_2:67829668-67829687 None:intergenic 60.0%
! AGCGTTGGGATTCAGCGAGG+AGG + chr5_2:67829671-67829690 None:intergenic 60.0%
CGGAACGAACATCGGAGCGT+TGG + chr5_2:67829686-67829705 None:intergenic 60.0%
TCCGCTTGCGTATCGTACGG+TGG - chr5_2:67829702-67829721 Msa0783680:three_prime_UTR 60.0%
GCTTGCGTATCGTACGGTGG+AGG - chr5_2:67829705-67829724 Msa0783680:three_prime_UTR 60.0%
GAAACCCAGAGTGAGCCCAC+GGG - chr5_2:67830221-67830240 Msa0783680:intron 60.0%
ATAGCCCGTGGGCTCACTCT+GGG + chr5_2:67830228-67830247 None:intergenic 60.0%
AATAGCCCGTGGGCTCACTC+TGG + chr5_2:67830229-67830248 None:intergenic 60.0%
CTGGGCTGATGAATAGCCCG+TGG + chr5_2:67830240-67830259 None:intergenic 60.0%
! CACGGGCTATTCATCAGCCC+AGG - chr5_2:67830238-67830257 Msa0783680:intron 60.0%
!! CGGAGCGTTGGGATTCAGCG+AGG + chr5_2:67829674-67829693 None:intergenic 65.0%
!! CGGAGCGTTGGGATTCAGCG+AGG + chr5_2:67829674-67829693 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 67829544 67830592 67829544 ID=Msa0783680;Name=Msa0783680
chr5_2 mRNA 67829544 67830592 67829544 ID=Msa0783680-mRNA-1;Parent=Msa0783680;Name=Msa0783680-mRNA-1;_AED=0.00;_eAED=0.00;_QI=93|1|1|1|1|1|2|329|134
chr5_2 exon 67829544 67830021 67829544 ID=Msa0783680-mRNA-1:exon:20338;Parent=Msa0783680-mRNA-1
chr5_2 exon 67830244 67830592 67830244 ID=Msa0783680-mRNA-1:exon:20337;Parent=Msa0783680-mRNA-1
chr5_2 five_prime_UTR 67830500 67830592 67830500 ID=Msa0783680-mRNA-1:five_prime_utr;Parent=Msa0783680-mRNA-1
chr5_2 CDS 67830244 67830499 67830244 ID=Msa0783680-mRNA-1:cds;Parent=Msa0783680-mRNA-1
chr5_2 CDS 67829873 67830021 67829873 ID=Msa0783680-mRNA-1:cds;Parent=Msa0783680-mRNA-1
chr5_2 three_prime_UTR 67829544 67829872 67829544 ID=Msa0783680-mRNA-1:three_prime_utr;Parent=Msa0783680-mRNA-1
Gene Sequence

>Msa0783680

ATGGAAGTTCTTTCTGCTACAAGAACATCCTCCTCCTCGCTGAATCCCAACGCTCCGATGTTCGTTCCGCTTGCGTATCGTACGGTGGAGGATTTCTCCGATGAATGGTGGAACCTCGTTCATTCCTCCCCTTGGTTCCGTGATTACTGGCTCCGTGAACGCTTCCAAGATCCTCAGAACTATCAAAATGACTCGTTTTCTGATTTTGAAATGATGGATGAAGATGATCTTTTCCATGTTCATGAAATTGAACCCCAACACGAAGATGGGAAACAGTTGATGAAATTGGGATCGTTGAAATGGAGAGGATCTGATGGATGGGCTGAAGTTCCAAGGTATGCTGAGAAGGCTCCGAAGATTGTGAAACCCAGAGTGAGCCCACGGGCTATTCATCAGCCCAGGTAG
Protein sequence

>Msa0783680

MEVLSATRTSSSSLNPNAPMFVPLAYRTVEDFSDEWWNLVHSSPWFRDYWLRERFQDPQNYQNDSFSDFEMMDEDDLFHVHEIEPQHEDGKQLMKLGSLKWRGSDGWAEVPRYAEKAPKIVKPRVSPRAIHQPR*