AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0898410


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0898410 MtrunA17_Chr6g0483981 81.481 135 23 1 1 133 1 135 1.12e-69 219
Msa0898410 MtrunA17_Chr4g0064041 67.188 128 40 2 1 126 1 128 5.12e-51 166
Msa0898410 MtrunA17_Chr4g0064041 58.416 101 42 0 26 126 190 290 9.82e-39 134
Msa0898410 MtrunA17_Chr2g0306461 45.833 120 63 2 8 127 9 126 3.53e-30 107
Msa0898410 MtrunA17_Chr4g0033131 39.024 123 73 2 7 127 8 130 3.00e-26 97.1
Msa0898410 MtrunA17_Chr4g0033531 39.024 123 73 2 7 127 8 130 3.00e-26 97.1
Msa0898410 MtrunA17_Chr3g0132441 41.322 121 66 3 6 126 5 120 3.23e-23 89.4
Msa0898410 MtrunA17_Chr1g0205701 35.714 126 77 2 1 126 1 122 9.12e-23 88.2
Msa0898410 MtrunA17_Chr5g0394321 39.048 105 62 1 22 126 21 123 1.19e-22 87.8
Msa0898410 MtrunA17_Chr3g0132431 38.211 123 71 3 6 128 5 122 9.53e-22 85.5
Msa0898410 MtrunA17_Chr8g0380901 38.710 124 67 3 6 127 5 121 1.96e-21 83.2
Msa0898410 MtrunA17_Chr8g0387804 34.167 120 78 1 8 127 10 128 3.95e-21 82.4
Msa0898410 MtrunA17_Chr8g0380711 36.607 112 62 2 16 127 20 122 1.37e-20 80.9
Msa0898410 MtrunA17_Chr3g0132701 42.453 106 56 3 22 126 21 122 5.00e-20 82.0
Msa0898410 MtrunA17_Chr3g0132511 42.453 106 56 3 22 126 21 122 6.76e-20 81.6
Msa0898410 MtrunA17_Chr2g0301431 41.346 104 54 3 22 125 27 123 2.15e-19 78.2
Msa0898410 MtrunA17_Chr7g0253641 42.056 107 58 2 22 128 21 123 4.35e-19 79.7
Msa0898410 MtrunA17_Chr7g0253761 39.815 108 59 2 22 128 21 123 7.84e-19 77.8
Msa0898410 MtrunA17_Chr8g0335571 41.905 105 54 3 24 126 21 120 9.30e-19 78.2
Msa0898410 MtrunA17_Chr8g0335531 42.857 105 53 4 24 126 21 120 1.87e-18 77.0
Msa0898410 MtrunA17_Chr7g0253631 40.000 110 62 2 22 131 21 126 9.59e-18 76.3
Msa0898410 MtrunA17_Chr4g0064031 37.113 97 58 3 24 117 25 121 2.46e-17 73.6
Msa0898410 MtrunA17_Chr2g0323021 40.506 79 46 1 47 125 42 119 5.92e-17 73.9
Msa0898410 MtrunA17_Chr7g0253681 35.246 122 71 3 9 126 6 123 9.74e-17 72.0
Msa0898410 MtrunA17_Chr3g0132411 36.000 125 74 3 3 127 42 160 1.47e-16 72.0
Msa0898410 MtrunA17_Chr6g0458331 34.653 101 63 2 27 126 37 135 3.30e-16 70.9
Msa0898410 MtrunA17_Chr7g0253701 35.537 121 71 3 9 126 6 122 7.78e-16 69.7
Msa0898410 MtrunA17_Chr7g0253671 35.537 121 71 3 9 126 6 122 7.78e-16 69.7
Msa0898410 MtrunA17_Chr3g0125791 36.634 101 57 2 24 124 30 123 1.18e-15 68.6
Msa0898410 MtrunA17_Chr1g0177341 34.454 119 71 3 11 126 7 121 1.43e-15 68.9
Msa0898410 MtrunA17_Chr1g0177331 34.454 119 71 3 11 126 7 121 1.43e-15 68.9
Msa0898410 MtrunA17_Chr2g0331181 31.496 127 84 2 1 126 1 125 1.55e-15 69.7
Msa0898410 MtrunA17_Chr3g0125761 32.500 120 74 3 8 127 14 126 4.29e-15 67.0
Msa0898410 MtrunA17_Chr1g0196801 36.538 104 61 2 24 127 20 118 5.49e-15 67.8
Msa0898410 MtrunA17_Chr1g0188981 34.653 101 65 1 27 126 29 129 6.63e-15 68.2
Msa0898410 MtrunA17_Chr7g0253731 33.333 111 68 2 12 120 8 114 1.18e-14 66.6
Msa0898410 MtrunA17_Chr1g0150131 31.193 109 67 2 35 135 34 142 3.34e-14 65.9
Msa0898410 MtrunA17_Chr1g0211101 28.455 123 83 2 8 129 14 132 2.61e-13 63.5
Msa0898410 MtrunA17_Chr4g0062341 28.099 121 82 3 8 126 14 131 5.08e-13 62.8
Msa0898410 MtrunA17_Chr4g0062311 28.099 121 82 3 8 126 14 131 5.08e-13 62.8
Msa0898410 MtrunA17_Chr2g0324721 31.200 125 72 3 24 137 24 145 2.42e-12 61.2
Msa0898410 MtrunA17_Chr8g0360371 31.624 117 76 2 8 124 7 119 2.95e-12 62.4
Msa0898410 MtrunA17_Chr4g0041811 28.037 107 77 0 18 124 23 129 2.98e-12 61.2
Msa0898410 MtrunA17_Chr3g0136871 31.034 116 69 2 24 128 24 139 1.41e-11 58.9
Msa0898410 MtrunA17_Chr4g0073901 26.562 128 93 1 1 127 3 130 3.31e-11 58.2
Msa0898410 MtrunA17_Chr6g0459441 34.286 105 63 4 23 126 22 121 4.42e-11 57.8
Msa0898410 MtrunA17_Chr2g0289741 28.148 135 85 5 7 137 5 131 6.05e-11 57.0
Msa0898410 MtrunA17_Chr4g0040291 32.039 103 68 1 24 126 22 122 7.43e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0898410 AT5G20230.1 48.039 102 52 1 25 126 23 123 7.54e-29 104
Msa0898410 AT3G17675.1 36.364 121 73 1 8 128 13 129 4.35e-23 87.8
Msa0898410 AT2G32300.1 39.062 128 73 3 1 128 1 123 9.86e-23 90.5
Msa0898410 AT2G32300.2 39.062 128 73 3 1 128 31 153 1.54e-22 90.5
Msa0898410 AT2G25060.1 41.525 118 65 3 22 135 26 143 1.70e-20 82.8
Msa0898410 AT5G26330.1 35.294 119 75 1 8 126 5 121 2.84e-20 82.4
Msa0898410 AT3G20570.1 34.400 125 78 2 1 122 1 124 7.18e-20 81.6
Msa0898410 AT2G26720.1 40.164 122 64 5 8 128 14 127 2.42e-19 80.1
Msa0898410 AT2G31050.1 38.211 123 72 2 12 133 13 132 4.98e-18 76.6
Msa0898410 AT3G60270.1 39.806 103 57 3 24 126 23 120 5.31e-18 76.3
Msa0898410 AT4G31840.1 36.364 121 75 2 8 126 7 127 1.15e-17 75.1
Msa0898410 AT3G60280.1 38.835 103 58 2 24 126 21 118 5.10e-16 72.0
Msa0898410 AT4G30590.1 31.933 119 79 2 8 124 8 126 1.86e-15 69.7
Msa0898410 AT5G07475.1 35.577 104 62 2 24 127 27 125 5.19e-15 68.6
Msa0898410 AT1G72230.1 40.244 82 48 1 45 126 37 117 7.00e-15 68.2
Msa0898410 AT2G44790.1 36.036 111 60 5 18 126 23 124 2.11e-14 67.4
Msa0898410 AT1G79800.1 30.645 124 79 3 11 129 14 135 2.43e-14 67.0
Msa0898410 AT1G22480.1 31.496 127 74 2 1 127 1 114 3.40e-14 66.2
Msa0898410 AT1G64640.1 31.707 123 79 2 8 126 10 131 4.73e-14 66.2
Msa0898410 AT2G02850.1 33.621 116 67 3 9 124 21 126 7.75e-14 64.3
Msa0898410 AT4G27520.1 33.333 114 74 1 11 124 15 126 1.25e-13 66.6
Msa0898410 AT3G18590.1 31.707 123 77 4 8 126 7 126 2.48e-13 64.3
Msa0898410 AT5G25090.1 35.052 97 63 0 30 126 30 126 5.67e-13 63.2
Msa0898410 AT1G45063.2 33.028 109 70 2 27 134 149 255 5.87e-13 64.3
Msa0898410 AT1G48940.1 32.381 105 67 2 27 129 26 128 7.91e-13 62.8
Msa0898410 AT1G45063.1 33.028 109 70 2 27 134 149 255 1.47e-12 63.5
Msa0898410 AT5G14345.1 26.891 119 81 3 9 126 1 114 1.80e-12 60.8
Msa0898410 AT4G28365.1 34.000 100 64 2 27 126 29 126 5.59e-12 60.8
Msa0898410 AT3G27200.1 29.752 121 77 4 9 126 6 121 8.20e-12 59.7
Msa0898410 AT4G32490.1 26.891 119 85 1 8 126 12 128 4.51e-11 58.5
Msa0898410 AT4G01380.1 34.694 98 61 3 34 129 79 175 7.62e-11 57.8
Msa0898410 AT3G53330.1 34.906 106 66 2 24 128 185 288 7.71e-11 58.5

Find 33 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTTCATATGTACCGTTAC+TGG 0.314883 6_1:+91974197 Msa0898410:CDS
ACCATGGCAAGCAACATAAA+TGG 0.379823 6_1:+91973791 None:intergenic
CTATATCAGTGGAAGGGTGC+TGG 0.436825 6_1:-91974275 None:intergenic
CTCATGAGAGACACCTTAGC+TGG 0.440760 6_1:-91974161 None:intergenic
GATCACAATTCTATGTCAAC+TGG 0.459472 6_1:+91973915 Msa0898410:CDS
GCAGTGGCTAACCTTCTTCA+TGG 0.479052 6_1:+91973833 Msa0898410:CDS
ACCATTTATGTTGCTTGCCA+TGG 0.484297 6_1:-91973792 None:intergenic
AAGGTGTCTCTCATGAGTGC+AGG 0.497615 6_1:+91974167 Msa0898410:CDS
CCGTGGGTGATGACTTAGGC+TGG 0.505012 6_1:+91973876 Msa0898410:CDS
GGCTGGATTATCCCAACCGT+TGG 0.506002 6_1:+91973893 Msa0898410:CDS
GTTACTGGTCACTGTTCTGC+TGG 0.511691 6_1:+91974212 Msa0898410:CDS
TAGAATTGTGATCCAACGGT+TGG 0.521337 6_1:-91973905 None:intergenic
TTGCTACGGATCCATGAAGA+AGG 0.528261 6_1:-91973844 None:intergenic
GAATGCACACGCCGTGGCAA+AGG 0.533207 6_1:+91974076 Msa0898410:CDS
AGAATTGTGATCCAACGGTT+GGG 0.536039 6_1:-91973904 None:intergenic
TCTGCTTTCGATAGTTGCAA+TGG 0.540088 6_1:+91974107 Msa0898410:CDS
CTTAAATCTATATCAGTGGA+AGG 0.546850 6_1:-91974282 None:intergenic
CAATGGAACCAACGCACTTG+TGG 0.564659 6_1:+91974124 Msa0898410:CDS
TAAGTCATCACCCACGGTGA+AGG 0.570272 6_1:-91973870 None:intergenic
TATGACAAAGCCTCCAGCTA+AGG 0.573588 6_1:+91974148 Msa0898410:CDS
GACATAGAATTGTGATCCAA+CGG 0.579776 6_1:-91973909 None:intergenic
AGCAGAACAGTGACCAGTAA+CGG 0.582060 6_1:-91974210 None:intergenic
TGTCATAACCACAAGTGCGT+TGG 0.590117 6_1:-91974132 None:intergenic
AGGTGTCTCTCATGAGTGCA+GGG 0.602357 6_1:+91974168 Msa0898410:CDS
TTCACCGTAGGTGACACTCT+TGG 0.604216 6_1:+91973953 Msa0898410:CDS
TTCACCGTGGGTGATGACTT+AGG 0.609147 6_1:+91973872 Msa0898410:CDS
TTAAATCTATATCAGTGGAA+GGG 0.611961 6_1:-91974281 None:intergenic
TCAATTGAATGCACACGCCG+TGG 0.614375 6_1:+91974070 Msa0898410:CDS
AAGAGTGTCACCTACGGTGA+AGG 0.615747 6_1:-91973951 None:intergenic
ACCAATAAAACCTTCACCGT+AGG 0.655037 6_1:+91973941 Msa0898410:CDS
ATGAGAGACACCTTAGCTGG+AGG 0.666881 6_1:-91974158 None:intergenic
CCAGCCTAAGTCATCACCCA+CGG 0.672113 6_1:-91973876 None:intergenic
CATACCAAGAGTGTCACCTA+CGG 0.673615 6_1:-91973957 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TATTTCATATGTACCGTTAC+TGG + chr6_1:91974197-91974216 Msa0898410:CDS 30.0%
GACATAGAATTGTGATCCAA+CGG - chr6_1:91973912-91973931 None:intergenic 35.0%
GATCACAATTCTATGTCAAC+TGG + chr6_1:91973915-91973934 Msa0898410:CDS 35.0%
! AGATTTTGTTACCTTTGCCA+CGG - chr6_1:91974090-91974109 None:intergenic 35.0%
! GGTGCTGGAAAAAAGATAAT+CGG - chr6_1:91974263-91974282 None:intergenic 35.0%
AGAATTGTGATCCAACGGTT+GGG - chr6_1:91973907-91973926 None:intergenic 40.0%
TAGAATTGTGATCCAACGGT+TGG - chr6_1:91973908-91973927 None:intergenic 40.0%
ACCAATAAAACCTTCACCGT+AGG + chr6_1:91973941-91973960 Msa0898410:CDS 40.0%
!! ACCTACGGTGAAGGTTTTAT+TGG - chr6_1:91973945-91973964 None:intergenic 40.0%
TCTGCTTTCGATAGTTGCAA+TGG + chr6_1:91974107-91974126 Msa0898410:CDS 40.0%
TTGCTACGGATCCATGAAGA+AGG - chr6_1:91973847-91973866 None:intergenic 45.0%
! CACGGTGAAGGTTTTTGCTA+CGG - chr6_1:91973861-91973880 None:intergenic 45.0%
GTAGCAAAAACCTTCACCGT+GGG + chr6_1:91973860-91973879 Msa0898410:CDS 45.0%
!! CATACCAAGAGTGTCACCTA+CGG - chr6_1:91973960-91973979 None:intergenic 45.0%
TGTCATAACCACAAGTGCGT+TGG - chr6_1:91974135-91974154 None:intergenic 45.0%
TATGACAAAGCCTCCAGCTA+AGG + chr6_1:91974148-91974167 Msa0898410:CDS 45.0%
AGCAGAACAGTGACCAGTAA+CGG - chr6_1:91974213-91974232 None:intergenic 45.0%
!! TGTTTTTGTGCTGCTCGCAG+TGG + chr6_1:91973817-91973836 Msa0898410:CDS 50.0%
GCAGTGGCTAACCTTCTTCA+TGG + chr6_1:91973833-91973852 Msa0898410:CDS 50.0%
CGTAGCAAAAACCTTCACCG+TGG + chr6_1:91973859-91973878 Msa0898410:CDS 50.0%
TAAGTCATCACCCACGGTGA+AGG - chr6_1:91973873-91973892 None:intergenic 50.0%
TTCACCGTGGGTGATGACTT+AGG + chr6_1:91973872-91973891 Msa0898410:CDS 50.0%
!! AAGAGTGTCACCTACGGTGA+AGG - chr6_1:91973954-91973973 None:intergenic 50.0%
TTCACCGTAGGTGACACTCT+TGG + chr6_1:91973953-91973972 Msa0898410:CDS 50.0%
TCAATTGAATGCACACGCCG+TGG + chr6_1:91974070-91974089 Msa0898410:CDS 50.0%
CAATGGAACCAACGCACTTG+TGG + chr6_1:91974124-91974143 Msa0898410:CDS 50.0%
ATGAGAGACACCTTAGCTGG+AGG - chr6_1:91974161-91974180 None:intergenic 50.0%
CTCATGAGAGACACCTTAGC+TGG - chr6_1:91974164-91974183 None:intergenic 50.0%
! AAGGTGTCTCTCATGAGTGC+AGG + chr6_1:91974167-91974186 Msa0898410:CDS 50.0%
AGGTGTCTCTCATGAGTGCA+GGG + chr6_1:91974168-91974187 Msa0898410:CDS 50.0%
GTTACTGGTCACTGTTCTGC+TGG + chr6_1:91974212-91974231 Msa0898410:CDS 50.0%
CCAGCCTAAGTCATCACCCA+CGG - chr6_1:91973879-91973898 None:intergenic 55.0%
GGCTGGATTATCCCAACCGT+TGG + chr6_1:91973893-91973912 Msa0898410:CDS 55.0%
CCGTGGGTGATGACTTAGGC+TGG + chr6_1:91973876-91973895 Msa0898410:CDS 60.0%
GAATGCACACGCCGTGGCAA+AGG + chr6_1:91974076-91974095 Msa0898410:CDS 60.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 91973794 91974292 91973794 ID=Msa0898410;Name=Msa0898410
chr6_1 mRNA 91973794 91974292 91973794 ID=Msa0898410-mRNA-1;Parent=Msa0898410;Name=Msa0898410-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|0|0|1|1|1|2|0|137
chr6_1 exon 91973794 91973974 91973794 ID=Msa0898410-mRNA-1:exon:15547;Parent=Msa0898410-mRNA-1
chr6_1 exon 91974060 91974292 91974060 ID=Msa0898410-mRNA-1:exon:15548;Parent=Msa0898410-mRNA-1
chr6_1 CDS 91973794 91973974 91973794 ID=Msa0898410-mRNA-1:cds;Parent=Msa0898410-mRNA-1
chr6_1 CDS 91974060 91974292 91974060 ID=Msa0898410-mRNA-1:cds;Parent=Msa0898410-mRNA-1
Gene Sequence

>Msa0898410

ATGGCAAGCAACATAAATGGTCTTGTTTTTGTGCTGCTCGCAGTGGCTAACCTTCTTCATGGATCCGTAGCAAAAACCTTCACCGTGGGTGATGACTTAGGCTGGATTATCCCAACCGTTGGATCACAATTCTATGTCAACTGGTCGACCAATAAAACCTTCACCGTAGGTGACACTCTTGTGTTCAACTTTCAATTGAATGCACACGCCGTGGCAAAGGTAACAAAATCTGCTTTCGATAGTTGCAATGGAACCAACGCACTTGTGGTTATGACAAAGCCTCCAGCTAAGGTGTCTCTCATGAGTGCAGGGCAACAATATTTCATATGTACCGTTACTGGTCACTGTTCTGCTGGTCAGAAACTGTCGATTAACGTTTCTCCGATTATCTTTTTTCCAGCACCCTTCCACTGA

Protein sequence

>Msa0898410

MASNINGLVFVLLAVANLLHGSVAKTFTVGDDLGWIIPTVGSQFYVNWSTNKTFTVGDTLVFNFQLNAHAVAKVTKSAFDSCNGTNALVVMTKPPAKVSLMSAGQQYFICTVTGHCSAGQKLSINVSPIIFFPAPFH*