AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0900020

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0900020 MtrunA17_Chr6g0486001 97.068 307 9 0 1 307 1 307 0.0 617
Msa0900020 MtrunA17_Chr2g0331671 53.994 313 132 7 1 306 2 309 9.19e-112 326
Msa0900020 MtrunA17_Chr6g0483441 38.854 314 164 6 1 306 1 294 1.00e-59 192
Msa0900020 MtrunA17_Chr4g0053001 40.867 323 172 8 1 307 12 331 2.41e-59 192
Msa0900020 MtrunA17_Chr8g0392551 41.801 311 161 8 1 306 1 296 4.48e-55 181
Msa0900020 MtrunA17_Chr4g0038691 37.580 314 161 11 1 294 6 304 2.66e-48 164
Msa0900020 MtrunA17_Chr3g0090061 27.885 312 178 11 25 294 29 335 5.63e-21 91.7
Msa0900020 MtrunA17_Chr3g0091821 32.335 167 96 4 125 289 25 176 4.85e-20 85.9
Msa0900020 MtrunA17_Chr8g0379281 37.037 81 51 0 135 215 106 186 3.74e-14 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0900020 AT3G24605.1 42.258 310 156 7 1 303 3 296 1.63e-71 223
Msa0900020 AT1G45688.1 38.776 343 161 9 1 301 1 336 6.99e-60 195
Msa0900020 AT5G42860.1 38.019 313 152 7 1 286 1 298 2.76e-58 190
Msa0900020 AT3G24600.1 39.414 307 177 6 1 304 2 302 3.51e-51 171
Msa0900020 AT1G45688.2 40.000 245 118 8 1 223 1 238 6.58e-41 143
Msa0900020 AT4G35170.1 32.632 285 168 8 15 287 8 280 1.12e-39 141
Msa0900020 AT2G41990.1 33.882 304 163 8 1 289 1 281 1.24e-39 141
Msa0900020 AT3G08490.1 24.883 213 152 5 99 304 57 268 1.21e-14 73.2

Find 87 sgRNAs with CRISPR-Local

Find 185 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTGTTTATAACCCTTATT+AGG 0.144732 6_1:-95424362 None:intergenic
GGAATTGAGATTTGAGAATT+TGG 0.176623 6_1:-95424323 None:intergenic
ATTAAAGATGGAAAATTTAA+TGG 0.191613 6_1:-95424479 None:intergenic
TACTGGCTTGAGATATATTA+AGG 0.194537 6_1:+95427480 Msa0900020:three_prime_UTR
ATAACCCTTATTAGGGGTTT+TGG 0.196172 6_1:-95424354 None:intergenic
GAAGGACTTTGAACGTAATA+AGG 0.197913 6_1:-95424612 None:intergenic
AAAACCCAAAACCCCTAATA+AGG 0.221938 6_1:+95424349 Msa0900020:five_prime_UTR
TGTGGGTTGGGATGAGAATT+TGG 0.245470 6_1:-95424416 None:intergenic
TTGAAGAGGAAGTGAAGAAT+TGG 0.281682 6_1:-95424272 None:intergenic
GATGAGAATTTGGTATTTGA+TGG 0.302879 6_1:-95424406 None:intergenic
ATACAACATCTAAGTGGTTT+TGG 0.309066 6_1:+95427415 Msa0900020:exon
TGCTGTTTGTGGGTTTGTTT+TGG 0.310858 6_1:+95424908 Msa0900020:CDS
AATAGAAAAGTGAATGATAA+AGG 0.318907 6_1:+95424789 Msa0900020:CDS
TCACCTTCACATCAATCTTA+TGG 0.323598 6_1:+95424693 Msa0900020:CDS
CATAACCCTGCAACTTTCTT+CGG 0.342827 6_1:+95426382 Msa0900020:CDS
TAAAGGGTGGATTGGATCTA+AGG 0.346851 6_1:+95424806 Msa0900020:CDS
ATTGTTTATAACCCTTATTA+GGG 0.348409 6_1:-95424361 None:intergenic
ACAGTACATAACTTCTACTT+TGG 0.351398 6_1:+95426289 Msa0900020:CDS
TGACAATTAAGAGGGTGTTT+AGG 0.362099 6_1:+95427529 Msa0900020:three_prime_UTR
AAGGACTTTGAACGTAATAA+GGG 0.376152 6_1:-95424611 None:intergenic
GCTATTTCAGTTCATTCTAC+TGG 0.384851 6_1:+95427463 Msa0900020:three_prime_UTR
GTTTGTGAATTGATTAAAGA+TGG 0.396112 6_1:-95424491 None:intergenic
TTTGATGTACTTGTTGTTGT+GGG 0.406752 6_1:-95427372 None:intergenic
TCATGAGAAGATTCTCTTGA+AGG 0.427779 6_1:-95424630 None:intergenic
ACTGGCTTGAGATATATTAA+GGG 0.432321 6_1:+95427481 Msa0900020:three_prime_UTR
TGAAGAGGAAGTGAAGAATT+GGG 0.434031 6_1:-95424271 None:intergenic
TAAATGCCGAAGAAAGTTGC+AGG 0.435222 6_1:-95426388 None:intergenic
GCTTAGTTATGACAATTAAG+AGG 0.436532 6_1:+95427520 Msa0900020:three_prime_UTR
TCTTCTTCAAGTAGAATCTC+TGG 0.444326 6_1:+95424732 Msa0900020:CDS
GAAGAAGAAAATGGTGGTTA+TGG 0.447068 6_1:+95424834 Msa0900020:CDS
GTGAATGATAAAGGGTGGAT+TGG 0.450115 6_1:+95424798 Msa0900020:CDS
AGATATATTAAGGGTTTGCT+TGG 0.453911 6_1:+95427490 Msa0900020:three_prime_UTR
TTCACTGTAAGCATCTTAGT+TGG 0.459875 6_1:-95426337 None:intergenic
GTTTGATGTACTTGTTGTTG+TGG 0.464472 6_1:-95427373 None:intergenic
AAACCCAAAACCCCTAATAA+GGG 0.467771 6_1:+95424350 Msa0900020:five_prime_UTR
GGTGAGAAGTGGAGTTGAAG+AGG 0.469307 6_1:-95424286 None:intergenic
GGAAGCAAGGTTCCATTGTA+TGG 0.470323 6_1:+95427203 Msa0900020:CDS
TGGTGCAGATGTTGCTAGCT+CGG 0.471095 6_1:+95427229 Msa0900020:CDS
GAAGTGAAGAATTGGGATCA+TGG 0.471548 6_1:-95424264 None:intergenic
GAGAATTTGGTATTTGATGG+AGG 0.496069 6_1:-95424403 None:intergenic
AAGTCGCGCGGAAATGTTGT+AGG 0.496383 6_1:+95427293 Msa0900020:CDS
AAGGTGATTGAAGAAGAAAA+TGG 0.496674 6_1:+95424825 Msa0900020:CDS
GTATATTGACAGACACTGTT+CGG 0.499883 6_1:-95427178 None:intergenic
TTTGTGATCTTGGTGATGAT+GGG 0.501451 6_1:-95424581 None:intergenic
CATAACTTCTACTTTGGAGA+AGG 0.514215 6_1:+95426295 Msa0900020:CDS
TTGAAGAAGAAGCTCTTGAA+TGG 0.526972 6_1:-95424719 None:intergenic
ATAGAAAAGTGAATGATAAA+GGG 0.527874 6_1:+95424790 Msa0900020:CDS
GAATTGGGATCATGGGAAAG+TGG 0.529774 6_1:-95424256 None:intergenic
GAATGAAGAGAGGTGAGAAG+TGG 0.530187 6_1:-95424297 None:intergenic
CGGAAATGTTGTAGGCAAGT+TGG 0.532289 6_1:+95427301 Msa0900020:CDS
AAGGTTCCATTGTATGGCGC+TGG 0.535461 6_1:+95427209 Msa0900020:CDS
AAGTGAAGAATTGGGATCAT+GGG 0.537782 6_1:-95424263 None:intergenic
TCAAGAGAATCTTCTCATGA+TGG 0.544641 6_1:+95424633 Msa0900020:CDS
GGAGAAGGGTCAGATGTCAC+TGG 0.551406 6_1:+95426310 Msa0900020:CDS
GAAAAGTGAATGATAAAGGG+TGG 0.557185 6_1:+95424793 Msa0900020:CDS
CTTAGTTATGACAATTAAGA+GGG 0.558237 6_1:+95427521 Msa0900020:three_prime_UTR
AACAATGCAACCAAGTCCAA+TGG 0.570691 6_1:+95424668 Msa0900020:CDS
TGATGTACTTGTTGTTGTGG+GGG 0.577495 6_1:-95427370 None:intergenic
ATACATAAGATTCACTGACT+TGG 0.579991 6_1:-95426419 None:intergenic
TTGATGTACTTGTTGTTGTG+GGG 0.580202 6_1:-95427371 None:intergenic
TGATGTGAAGGTGAATCCAT+TGG 0.581487 6_1:-95424684 None:intergenic
GAAGGTGAATCCATTGGACT+TGG 0.584702 6_1:-95424678 None:intergenic
GTGATCGAAATGGAGTTTCG+AGG 0.592967 6_1:+95424868 Msa0900020:CDS
TCTGATCTTACAGTTGCGAC+CGG 0.593421 6_1:+95426442 Msa0900020:CDS
AATTTGGACAGAATGAAGAG+AGG 0.595038 6_1:-95424307 None:intergenic
GTCTTTGAAGTTAAGTCGCG+CGG 0.601720 6_1:+95427281 Msa0900020:CDS
TGTCAATATACAAGGAAGCA+AGG 0.603269 6_1:+95427190 Msa0900020:CDS
ACAGTGTCTGTCAATATACA+AGG 0.604063 6_1:+95427182 Msa0900020:CDS
ATAACTTCTACTTTGGAGAA+GGG 0.607546 6_1:+95426296 Msa0900020:CDS
TCTGCACCAGCGCCATACAA+TGG 0.611769 6_1:-95427215 None:intergenic
TCGAAATGGAGTTTCGAGGA+GGG 0.621143 6_1:+95424872 Msa0900020:CDS
ATGTTGTAGGCAAGTTGGTG+AGG 0.621904 6_1:+95427306 Msa0900020:CDS
AATGAACTGAAATAGCTCAC+AGG 0.622836 6_1:-95427456 None:intergenic
GTGATTGAAGAAGAAAATGG+TGG 0.627521 6_1:+95424828 Msa0900020:CDS
TTGTTTATAACCCTTATTAG+GGG 0.629375 6_1:-95424360 None:intergenic
CAAACCTCAGCTCAGTGTCA+AGG 0.632858 6_1:+95424977 Msa0900020:CDS
AGGACTTTGAACGTAATAAG+GGG 0.639543 6_1:-95424610 None:intergenic
TATACCTTGACACTGAGCTG+AGG 0.640482 6_1:-95424981 None:intergenic
ATGTAGATACAACATCTAAG+TGG 0.640814 6_1:+95427409 Msa0900020:exon
TGACCATAAGATTGATGTGA+AGG 0.643898 6_1:-95424696 None:intergenic
TCTTCAAGTAGAATCTCTGG+TGG 0.645890 6_1:+95424735 Msa0900020:CDS
TGTTAGAATACTTACCTCGC+CGG 0.646947 6_1:-95426461 None:intergenic
ATCGAAATGGAGTTTCGAGG+AGG 0.650916 6_1:+95424871 Msa0900020:CDS
TATTGACAGACACTGTTCGG+CGG 0.651348 6_1:-95427175 None:intergenic
TCTTACAGTTGCGACCGGCG+AGG 0.653403 6_1:+95426447 Msa0900020:CDS
AAATGCCGAAGAAAGTTGCA+GGG 0.681577 6_1:-95426387 None:intergenic
GTTGCTAGCTCGGTGAACAA+CGG 0.683429 6_1:+95427239 Msa0900020:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTGTTTTTTTTAATCAA+AGG + chr6_1:95425363-95425382 Msa0900020:intron 10.0%
!!! TTTATAGTATATTTTTATAG+AGG + chr6_1:95427591-95427610 Msa0900020:three_prime_UTR 10.0%
!! ATTAAAGATGGAAAATTTAA+TGG - chr6_1:95424482-95424501 None:intergenic 15.0%
!!! GTTTTTTTATTATAGTTAGA+AGG + chr6_1:95425400-95425419 Msa0900020:intron 15.0%
!! AATGAAATTTCAAAAAAGTT+GGG - chr6_1:95425908-95425927 None:intergenic 15.0%
!! AAATGAAATTTCAAAAAAGT+TGG - chr6_1:95425909-95425928 None:intergenic 15.0%
!! ATTGTTTATAACCCTTATTA+GGG - chr6_1:95424364-95424383 None:intergenic 20.0%
!! AATTGTTTATAACCCTTATT+AGG - chr6_1:95424365-95424384 None:intergenic 20.0%
!! AATAGAAAAGTGAATGATAA+AGG + chr6_1:95424789-95424808 Msa0900020:CDS 20.0%
!! ATAGAAAAGTGAATGATAAA+GGG + chr6_1:95424790-95424809 Msa0900020:CDS 20.0%
!!! TTTGTTTTGTTTGATCATTT+GGG + chr6_1:95424938-95424957 Msa0900020:CDS 20.0%
!!! TCACAATTGTGTTTTAATTA+AGG + chr6_1:95425032-95425051 Msa0900020:intron 20.0%
!!! TTGATAGTTAAATTCATACT+AGG + chr6_1:95425536-95425555 Msa0900020:intron 20.0%
!!! CTTTTTTTTCAAAATCAATG+TGG - chr6_1:95425682-95425701 None:intergenic 20.0%
!!! TTTTAAAAAAATTGAGACAC+AGG - chr6_1:95426910-95426929 None:intergenic 20.0%
!! TGATAAACTTATAGATGTTT+AGG - chr6_1:95426949-95426968 None:intergenic 20.0%
! TTGTTTATAACCCTTATTAG+GGG - chr6_1:95424363-95424382 None:intergenic 25.0%
!!! CTTTAGGTTTGAATTTTTGT+GGG - chr6_1:95424436-95424455 None:intergenic 25.0%
! ATGAGAAAAATGTAAGCTTT+AGG - chr6_1:95424452-95424471 None:intergenic 25.0%
! GTTTGTGAATTGATTAAAGA+TGG - chr6_1:95424494-95424513 None:intergenic 25.0%
!!! TGGTTATAATAGCTCTTTTT+TGG + chr6_1:95424755-95424774 Msa0900020:CDS 25.0%
!!! GGTTATAATAGCTCTTTTTT+GGG + chr6_1:95424756-95424775 Msa0900020:CDS 25.0%
!!! GTTTGTTTTGTTTGATCATT+TGG + chr6_1:95424937-95424956 Msa0900020:CDS 25.0%
! CAGGAAAATATATGATCATT+TGG + chr6_1:95425485-95425504 Msa0900020:intron 25.0%
! ATTCATACTAGGAGATATTA+TGG + chr6_1:95425547-95425566 Msa0900020:intron 25.0%
! TTGAAAAAAAAGTTGCACTT+TGG + chr6_1:95425690-95425709 Msa0900020:intron 25.0%
! CTTAGTTATGACAATTAAGA+GGG + chr6_1:95427521-95427540 Msa0900020:three_prime_UTR 25.0%
GGAATTGAGATTTGAGAATT+TGG - chr6_1:95424326-95424345 None:intergenic 30.0%
GATGAGAATTTGGTATTTGA+TGG - chr6_1:95424409-95424428 None:intergenic 30.0%
!! GCTTTAGGTTTGAATTTTTG+TGG - chr6_1:95424437-95424456 None:intergenic 30.0%
! AAGCTTACATTTTTCTCATC+TGG + chr6_1:95424453-95424472 Msa0900020:five_prime_UTR 30.0%
! AGCTTACATTTTTCTCATCT+GGG + chr6_1:95424454-95424473 Msa0900020:five_prime_UTR 30.0%
AAGGACTTTGAACGTAATAA+GGG - chr6_1:95424614-95424633 None:intergenic 30.0%
AAGGTGATTGAAGAAGAAAA+TGG + chr6_1:95424825-95424844 Msa0900020:CDS 30.0%
!!! AGTTTTTCATTGCTGTTTGT+GGG + chr6_1:95424898-95424917 Msa0900020:CDS 30.0%
TATAAGAAACTTCCTTGGAT+TGG + chr6_1:95425000-95425019 Msa0900020:intron 30.0%
CTTGCAAAGCTTATAGAAAT+AGG + chr6_1:95425184-95425203 Msa0900020:intron 30.0%
!!! AAGCACTTTTAAGACTGTTT+GGG - chr6_1:95425256-95425275 None:intergenic 30.0%
!!! TAAGCACTTTTAAGACTGTT+TGG - chr6_1:95425257-95425276 None:intergenic 30.0%
ATACCAAAAGTCTACCATTT+AGG - chr6_1:95425318-95425337 None:intergenic 30.0%
GATATTATGGTTCAGCAAAA+TGG + chr6_1:95425560-95425579 Msa0900020:intron 30.0%
AAATTTCAAAAAAGTTGGGC+AGG - chr6_1:95425904-95425923 None:intergenic 30.0%
CATAGTTTATCACAGAACAA+TGG + chr6_1:95426072-95426091 Msa0900020:intron 30.0%
TTGTTAACTAGTAGCTATAG+TGG - chr6_1:95426129-95426148 None:intergenic 30.0%
TAGCTACTAGTTAACAAAAC+TGG + chr6_1:95426132-95426151 Msa0900020:intron 30.0%
TATCTATGTTATGTGAACAG+TGG + chr6_1:95426157-95426176 Msa0900020:intron 30.0%
ACAGTACATAACTTCTACTT+TGG + chr6_1:95426289-95426308 Msa0900020:CDS 30.0%
! ATAACTTCTACTTTGGAGAA+GGG + chr6_1:95426296-95426315 Msa0900020:CDS 30.0%
! ATACATAAGATTCACTGACT+TGG - chr6_1:95426422-95426441 None:intergenic 30.0%
GTTTCTAAATCAACAAGTAC+TGG - chr6_1:95426491-95426510 None:intergenic 30.0%
AGTTTCTCAAGTACTTATGT+CGG - chr6_1:95426587-95426606 None:intergenic 30.0%
TAAGTACTTGAGAAACTGTT+TGG + chr6_1:95426590-95426609 Msa0900020:intron 30.0%
AAGTACTTGAGAAACTGTTT+GGG + chr6_1:95426591-95426610 Msa0900020:intron 30.0%
AAATAAGATATAAGCTGTCC+TGG - chr6_1:95426639-95426658 None:intergenic 30.0%
!!! TGAACTCACTTTTGACTTAT+GGG - chr6_1:95427022-95427041 None:intergenic 30.0%
!!! TTGAACTCACTTTTGACTTA+TGG - chr6_1:95427023-95427042 None:intergenic 30.0%
GTCAAAAGTGAGTTCAAAAT+TGG + chr6_1:95427026-95427045 Msa0900020:intron 30.0%
ATGGAATTTGACAGTAAGAA+TGG - chr6_1:95427116-95427135 None:intergenic 30.0%
!! TTTGATGTACTTGTTGTTGT+GGG - chr6_1:95427375-95427394 None:intergenic 30.0%
ATGTAGATACAACATCTAAG+TGG + chr6_1:95427409-95427428 Msa0900020:exon 30.0%
! ATACAACATCTAAGTGGTTT+TGG + chr6_1:95427415-95427434 Msa0900020:exon 30.0%
!! TACTGGCTTGAGATATATTA+AGG + chr6_1:95427480-95427499 Msa0900020:three_prime_UTR 30.0%
!! ACTGGCTTGAGATATATTAA+GGG + chr6_1:95427481-95427500 Msa0900020:three_prime_UTR 30.0%
! AGATATATTAAGGGTTTGCT+TGG + chr6_1:95427490-95427509 Msa0900020:three_prime_UTR 30.0%
GCTTAGTTATGACAATTAAG+AGG + chr6_1:95427520-95427539 Msa0900020:three_prime_UTR 30.0%
AAGTGAAGAATTGGGATCAT+GGG - chr6_1:95424266-95424285 None:intergenic 35.0%
TGAAGAGGAAGTGAAGAATT+GGG - chr6_1:95424274-95424293 None:intergenic 35.0%
TTGAAGAGGAAGTGAAGAAT+TGG - chr6_1:95424275-95424294 None:intergenic 35.0%
AATTTGGACAGAATGAAGAG+AGG - chr6_1:95424310-95424329 None:intergenic 35.0%
AAAACCCAAAACCCCTAATA+AGG + chr6_1:95424349-95424368 Msa0900020:five_prime_UTR 35.0%
AAACCCAAAACCCCTAATAA+GGG + chr6_1:95424350-95424369 Msa0900020:five_prime_UTR 35.0%
! TAACCCTTATTAGGGGTTTT+GGG - chr6_1:95424356-95424375 None:intergenic 35.0%
! ATAACCCTTATTAGGGGTTT+TGG - chr6_1:95424357-95424376 None:intergenic 35.0%
GAGAATTTGGTATTTGATGG+AGG - chr6_1:95424406-95424425 None:intergenic 35.0%
! GGTTTGAATTTTTGTGGGTT+GGG - chr6_1:95424431-95424450 None:intergenic 35.0%
! AGGTTTGAATTTTTGTGGGT+TGG - chr6_1:95424432-95424451 None:intergenic 35.0%
!! TTTGTGATCTTGGTGATGAT+GGG - chr6_1:95424584-95424603 None:intergenic 35.0%
!!! TTTTGTGATCTTGGTGATGA+TGG - chr6_1:95424585-95424604 None:intergenic 35.0%
AGGACTTTGAACGTAATAAG+GGG - chr6_1:95424613-95424632 None:intergenic 35.0%
GAAGGACTTTGAACGTAATA+AGG - chr6_1:95424615-95424634 None:intergenic 35.0%
TCATGAGAAGATTCTCTTGA+AGG - chr6_1:95424633-95424652 None:intergenic 35.0%
TCAAGAGAATCTTCTCATGA+TGG + chr6_1:95424633-95424652 Msa0900020:CDS 35.0%
TCACCTTCACATCAATCTTA+TGG + chr6_1:95424693-95424712 Msa0900020:CDS 35.0%
TGACCATAAGATTGATGTGA+AGG - chr6_1:95424699-95424718 None:intergenic 35.0%
TTGAAGAAGAAGCTCTTGAA+TGG - chr6_1:95424722-95424741 None:intergenic 35.0%
TCTTCTTCAAGTAGAATCTC+TGG + chr6_1:95424732-95424751 Msa0900020:CDS 35.0%
GAAAAGTGAATGATAAAGGG+TGG + chr6_1:95424793-95424812 Msa0900020:CDS 35.0%
GTGATTGAAGAAGAAAATGG+TGG + chr6_1:95424828-95424847 Msa0900020:CDS 35.0%
GAAGAAGAAAATGGTGGTTA+TGG + chr6_1:95424834-95424853 Msa0900020:CDS 35.0%
!! GATTTTGATCGTGATCGAAA+TGG + chr6_1:95424858-95424877 Msa0900020:CDS 35.0%
!!! CAGTTTTTCATTGCTGTTTG+TGG + chr6_1:95424897-95424916 Msa0900020:CDS 35.0%
CAAGGTATAAGAAACTTCCT+TGG + chr6_1:95424995-95425014 Msa0900020:intron 35.0%
!!! GTTTTAATTAAGGCTCTGCT+TGG + chr6_1:95425042-95425061 Msa0900020:intron 35.0%
AATAGGCTGAAAACAGCTTA+TGG + chr6_1:95425201-95425220 Msa0900020:intron 35.0%
TCAATCCATACAGTCCTAAA+TGG + chr6_1:95425301-95425320 Msa0900020:intron 35.0%
! AGTCCTAAATGGTAGACTTT+TGG + chr6_1:95425312-95425331 Msa0900020:intron 35.0%
AAATAAAGTACACGAGGCAA+AGG - chr6_1:95425792-95425811 None:intergenic 35.0%
TTTGCCTCGTGTACTTTATT+TGG + chr6_1:95425791-95425810 Msa0900020:intron 35.0%
AGTACCAAATAAAGTACACG+AGG - chr6_1:95425798-95425817 None:intergenic 35.0%
GGCAAGATTGTAACATAACA+AGG - chr6_1:95425963-95425982 None:intergenic 35.0%
TGTTACAATCTTGCCAATCA+CGG + chr6_1:95425968-95425987 Msa0900020:intron 35.0%
! CATAACTTCTACTTTGGAGA+AGG + chr6_1:95426295-95426314 Msa0900020:CDS 35.0%
TTCACTGTAAGCATCTTAGT+TGG - chr6_1:95426340-95426359 None:intergenic 35.0%
ATTAAGCTGTTCATCCAAAC+AGG + chr6_1:95426749-95426768 Msa0900020:intron 35.0%
!! AAAAAAGTTCTAGGCCTGTT+TGG - chr6_1:95426766-95426785 None:intergenic 35.0%
!! AGTTCAAAATTGGCCTTTGA+AGG + chr6_1:95427036-95427055 Msa0900020:intron 35.0%
GTATATTGACAGACACTGTT+CGG - chr6_1:95427181-95427200 None:intergenic 35.0%
ACAGTGTCTGTCAATATACA+AGG + chr6_1:95427182-95427201 Msa0900020:CDS 35.0%
TGTCAATATACAAGGAAGCA+AGG + chr6_1:95427190-95427209 Msa0900020:CDS 35.0%
!! TTGATGTACTTGTTGTTGTG+GGG - chr6_1:95427374-95427393 None:intergenic 35.0%
!! GTTTGATGTACTTGTTGTTG+TGG - chr6_1:95427376-95427395 None:intergenic 35.0%
AATGAACTGAAATAGCTCAC+AGG - chr6_1:95427459-95427478 None:intergenic 35.0%
! GCTATTTCAGTTCATTCTAC+TGG + chr6_1:95427463-95427482 Msa0900020:three_prime_UTR 35.0%
!! TGACAATTAAGAGGGTGTTT+AGG + chr6_1:95427529-95427548 Msa0900020:three_prime_UTR 35.0%
GAAGTGAAGAATTGGGATCA+TGG - chr6_1:95424267-95424286 None:intergenic 40.0%
!!! TTAGGGGTTTTGGGTTTTGA+AGG - chr6_1:95424347-95424366 None:intergenic 40.0%
!!! GGGGTTTGTTTTTGTGATCT+TGG - chr6_1:95424594-95424613 None:intergenic 40.0%
AACAATGCAACCAAGTCCAA+TGG + chr6_1:95424668-95424687 Msa0900020:CDS 40.0%
TGATGTGAAGGTGAATCCAT+TGG - chr6_1:95424687-95424706 None:intergenic 40.0%
TCTTCAAGTAGAATCTCTGG+TGG + chr6_1:95424735-95424754 Msa0900020:CDS 40.0%
GTGAATGATAAAGGGTGGAT+TGG + chr6_1:95424798-95424817 Msa0900020:CDS 40.0%
!! TAAAGGGTGGATTGGATCTA+AGG + chr6_1:95424806-95424825 Msa0900020:CDS 40.0%
!! TGCTGTTTGTGGGTTTGTTT+TGG + chr6_1:95424908-95424927 Msa0900020:CDS 40.0%
AGACTGTTTGGGAAAGCTTA+TGG - chr6_1:95425245-95425264 None:intergenic 40.0%
! GTCTACCATTTAGGACTGTA+TGG - chr6_1:95425309-95425328 None:intergenic 40.0%
ATGCCAAATCCAAGACAAGA+GGG - chr6_1:95425454-95425473 None:intergenic 40.0%
! TTTGGCATGATGTTTGAGAC+AGG + chr6_1:95425466-95425485 Msa0900020:intron 40.0%
GTTCTGTTCAAGTGCATACA+TGG + chr6_1:95425624-95425643 Msa0900020:intron 40.0%
! CAAGTGCATACATGGATTGA+CGG + chr6_1:95425632-95425651 Msa0900020:intron 40.0%
! ACATGGATTGACGGTGAATT+TGG + chr6_1:95425641-95425660 Msa0900020:intron 40.0%
GTTTGGATTGACTGAGAGTT+GGG - chr6_1:95425751-95425770 None:intergenic 40.0%
TGTTTGGATTGACTGAGAGT+TGG - chr6_1:95425752-95425771 None:intergenic 40.0%
TTTGCATAACCTGCTTCATG+AGG + chr6_1:95425856-95425875 Msa0900020:intron 40.0%
TTGCATAACCTGCTTCATGA+GGG + chr6_1:95425857-95425876 Msa0900020:intron 40.0%
GTTACAATCTTGCCAATCAC+GGG + chr6_1:95425969-95425988 Msa0900020:intron 40.0%
CATAACCCTGCAACTTTCTT+CGG + chr6_1:95426382-95426401 Msa0900020:CDS 40.0%
AAATGCCGAAGAAAGTTGCA+GGG - chr6_1:95426390-95426409 None:intergenic 40.0%
TAAATGCCGAAGAAAGTTGC+AGG - chr6_1:95426391-95426410 None:intergenic 40.0%
TGTTAGAATACTTACCTCGC+CGG - chr6_1:95426464-95426483 None:intergenic 40.0%
!!! CAGGCCTAGAACTTTTTTGA+CGG + chr6_1:95426768-95426787 Msa0900020:intron 40.0%
CTGTCCGTCAAAAAAGTTCT+AGG - chr6_1:95426775-95426794 None:intergenic 40.0%
!! AAAATTGGCCTTTGAAGGAG+TGG + chr6_1:95427041-95427060 Msa0900020:intron 40.0%
CATTTAAGCCACTCCTTCAA+AGG - chr6_1:95427052-95427071 None:intergenic 40.0%
TTTCTTCAACTGCAGCAACA+TGG - chr6_1:95427135-95427154 None:intergenic 40.0%
!! TGATGTACTTGTTGTTGTGG+GGG - chr6_1:95427373-95427392 None:intergenic 40.0%
GAATTGGGATCATGGGAAAG+TGG - chr6_1:95424259-95424278 None:intergenic 45.0%
GAATGAAGAGAGGTGAGAAG+TGG - chr6_1:95424300-95424319 None:intergenic 45.0%
!! TGTGGGTTGGGATGAGAATT+TGG - chr6_1:95424419-95424438 None:intergenic 45.0%
GAAGGTGAATCCATTGGACT+TGG - chr6_1:95424681-95424700 None:intergenic 45.0%
GTGATCGAAATGGAGTTTCG+AGG + chr6_1:95424868-95424887 Msa0900020:CDS 45.0%
ATCGAAATGGAGTTTCGAGG+AGG + chr6_1:95424871-95424890 Msa0900020:CDS 45.0%
TCGAAATGGAGTTTCGAGGA+GGG + chr6_1:95424872-95424891 Msa0900020:CDS 45.0%
TATACCTTGACACTGAGCTG+AGG - chr6_1:95424984-95425003 None:intergenic 45.0%
TGAAGCAAGTAGCCAATCCA+AGG - chr6_1:95425015-95425034 None:intergenic 45.0%
TTAAGACAGCCCTCTTGTCT+TGG + chr6_1:95425442-95425461 Msa0900020:intron 45.0%
CATGCCAAATCCAAGACAAG+AGG - chr6_1:95425455-95425474 None:intergenic 45.0%
!! TGCACTTTGGAGCTTCAAAG+AGG + chr6_1:95425703-95425722 Msa0900020:intron 45.0%
GCTAAGCACGAAAAATGGCA+GGG - chr6_1:95425933-95425952 None:intergenic 45.0%
TCGACGCTAAGCACGAAAAA+TGG - chr6_1:95425938-95425957 None:intergenic 45.0%
!!! CTATTTTTGCGACCCGTGAT+TGG - chr6_1:95425984-95426003 None:intergenic 45.0%
TCTGATCTTACAGTTGCGAC+CGG + chr6_1:95426442-95426461 Msa0900020:CDS 45.0%
CTTATGTCTGTAAGCTCACC+AGG + chr6_1:95426618-95426637 Msa0900020:intron 45.0%
TATTGACAGACACTGTTCGG+CGG - chr6_1:95427178-95427197 None:intergenic 45.0%
GGAAGCAAGGTTCCATTGTA+TGG + chr6_1:95427203-95427222 Msa0900020:CDS 45.0%
!! GTCTTTGAAGTTAAGTCGCG+CGG + chr6_1:95427281-95427300 Msa0900020:CDS 45.0%
CGGAAATGTTGTAGGCAAGT+TGG + chr6_1:95427301-95427320 Msa0900020:CDS 45.0%
!! ATGTTGTAGGCAAGTTGGTG+AGG + chr6_1:95427306-95427325 Msa0900020:CDS 45.0%
!!! TTTTTTATATAAACTATATA+TGG - chr6_1:95427620-95427639 None:intergenic 5.0%
GGTGAGAAGTGGAGTTGAAG+AGG - chr6_1:95424289-95424308 None:intergenic 50.0%
CAAACCTCAGCTCAGTGTCA+AGG + chr6_1:95424977-95424996 Msa0900020:CDS 50.0%
CAGCCCTCTTGTCTTGGATT+TGG + chr6_1:95425448-95425467 Msa0900020:intron 50.0%
CAATCCAAACATGCACCGAG+TGG + chr6_1:95425761-95425780 Msa0900020:intron 50.0%
!! TACACCACTCGGTGCATGTT+TGG - chr6_1:95425768-95425787 None:intergenic 50.0%
GAGGCAAAGGATACACCACT+CGG - chr6_1:95425779-95425798 None:intergenic 50.0%
ATACAACGCCCTCATGAAGC+AGG - chr6_1:95425868-95425887 None:intergenic 50.0%
!! TGCTTCATGAGGGCGTTGTA+TGG + chr6_1:95425867-95425886 Msa0900020:intron 50.0%
CGCTAAGCACGAAAAATGGC+AGG - chr6_1:95425934-95425953 None:intergenic 50.0%
AAGGTTCCATTGTATGGCGC+TGG + chr6_1:95427209-95427228 Msa0900020:CDS 50.0%
TGGTGCAGATGTTGCTAGCT+CGG + chr6_1:95427229-95427248 Msa0900020:CDS 50.0%
! GTTGCTAGCTCGGTGAACAA+CGG + chr6_1:95427239-95427258 Msa0900020:CDS 50.0%
AAGTCGCGCGGAAATGTTGT+AGG + chr6_1:95427293-95427312 Msa0900020:CDS 50.0%
!! CTTGTTGTTGTGGGGGTCAA+CGG - chr6_1:95427366-95427385 None:intergenic 50.0%
GGAGAAGGGTCAGATGTCAC+TGG + chr6_1:95426310-95426329 Msa0900020:CDS 55.0%
TCTGCACCAGCGCCATACAA+TGG - chr6_1:95427218-95427237 None:intergenic 55.0%
TCTTACAGTTGCGACCGGCG+AGG + chr6_1:95426447-95426466 Msa0900020:CDS 60.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 95424249 95427691 95424249 ID=Msa0900020;Name=Msa0900020
chr6_1 mRNA 95424249 95427691 95424249 ID=Msa0900020-mRNA-1;Parent=Msa0900020;Name=Msa0900020-mRNA-1;_AED=0.10;_eAED=0.10;_QI=294|1|1|1|1|1|3|264|307
chr6_1 exon 95424249 95424998 95424249 ID=Msa0900020-mRNA-1:exon:16453;Parent=Msa0900020-mRNA-1
chr6_1 exon 95426283 95426468 95426283 ID=Msa0900020-mRNA-1:exon:16454;Parent=Msa0900020-mRNA-1
chr6_1 exon 95427146 95427691 95427146 ID=Msa0900020-mRNA-1:exon:16455;Parent=Msa0900020-mRNA-1
chr6_1 five_prime_UTR 95424249 95424542 95424249 ID=Msa0900020-mRNA-1:five_prime_utr;Parent=Msa0900020-mRNA-1
chr6_1 CDS 95424543 95424998 95424543 ID=Msa0900020-mRNA-1:cds;Parent=Msa0900020-mRNA-1
chr6_1 CDS 95426283 95426468 95426283 ID=Msa0900020-mRNA-1:cds;Parent=Msa0900020-mRNA-1
chr6_1 CDS 95427146 95427427 95427146 ID=Msa0900020-mRNA-1:cds;Parent=Msa0900020-mRNA-1
chr6_1 three_prime_UTR 95427428 95427691 95427428 ID=Msa0900020-mRNA-1:three_prime_utr;Parent=Msa0900020-mRNA-1
Gene Sequence

>Msa0900020

ATGCACACAAAAACTGATTCTGATGTCACAAGCTTCGACCCATCATCACCAAGATCACAAAAACAAACCCCTTATTACGTTCAAAGTCCTTCAAGAGAATCTTCTCATGATGGTGATAAATCTTCAACAATGCAACCAAGTCCAATGGATTCACCTTCACATCAATCTTATGGTCACCATTCAAGAGCTTCTTCTTCAAGTAGAATCTCTGGTGGTTATAATAGCTCTTTTTTGGGTAAGAAAGTGAATAGAAAAGTGAATGATAAAGGGTGGATTGGATCTAAGGTGATTGAAGAAGAAAATGGTGGTTATGGTGATTTTGATCGTGATCGAAATGGAGTTTCGAGGAGGGTTCAGTTTTTCATTGCTGTTTGTGGGTTTGTTTTGGTTTTTAGTTTGTTTTGTTTGATCATTTGGGCTGCTAGCTTGCATTACAAACCTCAGCTCAGTGTCAAGAGTTTGACAGTACATAACTTCTACTTTGGAGAAGGGTCAGATGTCACTGGAGTTCCAACTAAGATGCTTACAGTGAATTGTTCAATGAGAATGTTAGTGCATAACCCTGCAACTTTCTTCGGCATTTATGTCAGCTCCAAGTCAGTGAATCTTATGTATTCTGATCTTACAGTTGCGACCGGCGAGTTGAAGAAATATCATCAACAGAGAAAGAGCCGCCGAACAGTGTCTGTCAATATACAAGGAAGCAAGGTTCCATTGTATGGCGCTGGTGCAGATGTTGCTAGCTCGGTGAACAACGGTAAAGTATCGTTAACGCTTGTCTTTGAAGTTAAGTCGCGCGGAAATGTTGTAGGCAAGTTGGTGAGGACTAAGCACACACAGCGTGTCTCTTGCTCAATAGCCGTTGACCCCCACAACAACAAGTACATCAAACTTAAAGAGAATGAATGTAGATACAACATCTAA
Protein sequence

>Msa0900020

MHTKTDSDVTSFDPSSPRSQKQTPYYVQSPSRESSHDGDKSSTMQPSPMDSPSHQSYGHHSRASSSSRISGGYNSSFLGKKVNRKVNDKGWIGSKVIEEENGGYGDFDRDRNGVSRRVQFFIAVCGFVLVFSLFCLIIWAASLHYKPQLSVKSLTVHNFYFGEGSDVTGVPTKMLTVNCSMRMLVHNPATFFGIYVSSKSVNLMYSDLTVATGELKKYHQQRKSRRTVSVNIQGSKVPLYGAGADVASSVNNGKVSLTLVFEVKSRGNVVGKLVRTKHTQRVSCSIAVDPHNNKYIKLKENECRYNI*