AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0925660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0925660 MtrunA17_Chr6g0488471 95.254 295 8 2 1 295 1 289 0.0 565
Msa0925660 MtrunA17_Chr8g0389241 50.160 313 105 12 1 295 1 280 1.10e-73 227
Msa0925660 MtrunA17_Chr2g0331031 53.073 179 62 4 1 162 1 174 1.06e-48 165
Msa0925660 MtrunA17_Chr4g0055931 48.108 185 74 4 1 175 1 173 2.54e-47 161
Msa0925660 MtrunA17_Chr5g0415191 48.555 173 70 4 1 162 3 167 2.17e-41 145
Msa0925660 MtrunA17_Chr3g0135671 44.565 184 82 5 1 177 3 173 2.55e-36 130
Msa0925660 MtrunA17_Chr1g0162081 60.784 102 33 2 91 185 116 217 4.57e-34 125
Msa0925660 MtrunA17_Chr4g0062741 73.239 71 19 0 91 161 132 202 9.53e-34 125
Msa0925660 MtrunA17_Chr7g0241761 78.873 71 15 0 91 161 134 204 3.38e-33 124
Msa0925660 MtrunA17_Chr4g0059701 48.780 164 69 5 32 187 15 171 1.47e-31 119
Msa0925660 MtrunA17_Chr3g0104071 73.611 72 19 0 91 162 141 212 2.00e-31 119
Msa0925660 MtrunA17_Chr5g0429521 73.611 72 19 0 91 162 126 197 3.12e-31 118
Msa0925660 MtrunA17_Chr1g0210471 71.429 70 20 0 92 161 119 188 2.98e-30 114
Msa0925660 MtrunA17_Chr5g0436501 69.863 73 20 1 91 161 119 191 5.81e-29 111
Msa0925660 MtrunA17_Chr3g0092621 60.976 82 30 1 82 161 67 148 1.69e-28 107
Msa0925660 MtrunA17_Chr6g0452791 67.797 59 19 0 97 155 80 138 1.92e-22 91.7
Msa0925660 MtrunA17_Chr5g0409841 52.857 70 31 1 93 160 88 157 3.62e-19 84.3
Msa0925660 MtrunA17_Chr5g0440731 50.704 71 32 1 93 160 101 171 6.08e-18 82.0
Msa0925660 MtrunA17_Chr5g0440741 55.385 65 26 1 99 160 102 166 1.64e-17 80.9
Msa0925660 MtrunA17_Chr5g0409831 58.929 56 23 0 93 148 73 128 1.18e-16 77.4
Msa0925660 MtrunA17_Chr8g0365011 51.948 77 23 2 99 161 94 170 4.03e-15 73.9
Msa0925660 MtrunA17_Chr1g0192061 50.633 79 33 3 99 174 63 138 4.62e-15 72.0
Msa0925660 MtrunA17_Chr8g0364731 64.912 57 18 1 107 161 11 67 4.67e-15 72.0
Msa0925660 MtrunA17_Chr5g0432921 47.500 80 38 3 99 175 115 193 2.14e-12 65.5
Msa0925660 MtrunA17_Chr5g0432941 46.250 80 39 3 99 175 115 193 7.13e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0925660 AT5G56840.1 55.838 197 54 6 1 185 1 176 8.15e-61 193
Msa0925660 AT5G47390.1 52.907 172 60 4 1 162 1 161 2.82e-49 167
Msa0925660 AT5G61620.1 49.123 171 71 6 1 161 8 172 7.55e-46 157
Msa0925660 AT3G16350.1 40.323 310 117 11 1 257 1 295 3.43e-43 152
Msa0925660 AT1G70000.1 50.857 175 61 7 1 162 1 163 1.38e-42 147
Msa0925660 AT1G70000.2 50.857 175 61 7 1 162 1 163 1.38e-42 147
Msa0925660 AT1G49010.1 66.667 81 27 0 91 171 128 208 1.96e-33 124
Msa0925660 AT5G08520.1 67.949 78 25 0 91 168 112 189 6.72e-33 123
Msa0925660 AT2G38090.1 71.250 80 21 1 85 162 127 206 1.72e-32 122
Msa0925660 AT5G58900.1 60.417 96 38 0 92 187 136 231 7.39e-31 117
Msa0925660 AT5G01200.1 70.423 71 21 0 91 161 140 210 1.07e-29 114
Msa0925660 AT3G11280.2 69.014 71 22 0 91 161 121 191 1.82e-28 110
Msa0925660 AT3G11280.1 69.014 71 22 0 91 161 121 191 1.82e-28 110
Msa0925660 AT5G04760.1 65.714 70 24 0 92 161 94 163 3.83e-28 108
Msa0925660 AT5G05790.2 67.606 71 23 0 91 161 125 195 1.38e-27 108
Msa0925660 AT5G05790.1 67.606 71 23 0 91 161 125 195 1.38e-27 108
Msa0925660 AT1G19000.2 41.294 201 88 7 93 291 98 270 8.18e-27 106
Msa0925660 AT1G19000.1 41.294 201 88 7 93 291 98 270 8.18e-27 106
Msa0925660 AT1G74840.2 46.259 147 68 4 23 161 17 160 1.04e-26 105
Msa0925660 AT1G74840.1 46.259 147 68 4 23 161 17 160 2.16e-26 105
Msa0925660 AT5G23650.1 60.563 71 28 0 91 161 114 184 2.98e-23 97.8
Msa0925660 AT3G10580.2 56.627 83 34 1 93 175 91 171 7.29e-23 95.5
Msa0925660 AT3G10580.1 58.228 79 31 1 93 171 91 167 1.12e-22 95.5
Msa0925660 AT4G09450.1 57.971 69 29 0 93 161 87 155 8.05e-20 85.9
Msa0925660 AT3G10590.1 51.316 76 34 2 89 162 101 175 7.44e-16 75.1

Find 64 sgRNAs with CRISPR-Local

Find 242 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCACACCAACACTATTTAAT+TGG 0.185566 6_2:+68930757 None:intergenic
GCTAATTAAGTCACACTAAT+TGG 0.200852 6_2:+68928569 None:intergenic
AATTACAAGTCCTTGAATTA+TGG 0.245021 6_2:+68930529 None:intergenic
TCCCCAAGTTCATTTCTTAT+TGG 0.248835 6_2:-68928593 Msa0925660:CDS
GCTGCTACATTAGAAGAATT+TGG 0.271044 6_2:+68928677 None:intergenic
CCCCAAGTTCATTTCTTATT+GGG 0.272509 6_2:-68928592 Msa0925660:CDS
TTGTTCCATGTTATTTGAAT+TGG 0.302002 6_2:+68928625 None:intergenic
CATATCATAATAATCTGATT+TGG 0.337561 6_2:+68928811 None:intergenic
TTGAAAAGTTGCATACATAA+AGG 0.346393 6_2:+68928515 None:intergenic
TTGTGTTTATTGTCTCTTAG+AGG 0.354122 6_2:+68930634 None:intergenic
GCGGTGGAATTTGTGAAAAT+TGG 0.355606 6_2:+68930732 None:intergenic
ATATTTAAAGACTTTGTTAG+AGG 0.361817 6_2:+68928713 None:intergenic
AGAAGCTTGGAAAGGGAGAT+TGG 0.367189 6_2:-68929949 Msa0925660:CDS
CTATGCTCTTCTTCTGTCCA+TGG 0.368105 6_2:+68929992 None:intergenic
ACCAACACTATTTAATTGGT+TGG 0.373969 6_2:+68930761 None:intergenic
ACGTCTCTTCTTCTTGTTCA+AGG 0.393113 6_2:+68929841 None:intergenic
AGGACTTGTAATTCATTAAG+AGG 0.396462 6_2:-68930519 Msa0925660:CDS
AGAGACAATAAACACAAGTT+TGG 0.398819 6_2:-68930628 Msa0925660:five_prime_UTR
GAGCATAGAAAATTCCTTGT+TGG 0.413219 6_2:-68929977 Msa0925660:CDS
CTTGTTGGACTTGAGAAGCT+TGG 0.417301 6_2:-68929962 Msa0925660:CDS
GTTAGAGGATGTAGCCAATC+TGG 0.418818 6_2:+68928728 None:intergenic
GCTTGGAAAGGGAGATTGGA+GGG 0.428206 6_2:-68929945 Msa0925660:CDS
ATTGATGATAGAGCCTCTAT+TGG 0.438397 6_2:-68930348 Msa0925660:CDS
CACAAAAGAACTTATGTGTC+TGG 0.441747 6_2:+68930681 None:intergenic
AACATAAAGATGTGCCAGAT+TGG 0.460301 6_2:-68928742 Msa0925660:CDS
GAAGAAGAAAAGGAAGAAGT+AGG 0.461242 6_2:+68930381 None:intergenic
TCTATTGGTTATCTTTCAGA+TGG 0.477044 6_2:-68930333 Msa0925660:CDS
GGATAATCTTGAAGAAGAAA+AGG 0.479396 6_2:+68930371 None:intergenic
GTGATATATATGATGATTGA+TGG 0.479509 6_2:+68930703 None:intergenic
GATCTTGAGCTAACTCTGGC+AGG 0.479985 6_2:-68928650 Msa0925660:CDS
TTTCTTGTGTACGAACTATG+AGG 0.489619 6_2:+68930310 None:intergenic
AATAGAGGCTCTATCATCAA+TGG 0.491413 6_2:+68930350 None:intergenic
GAAACATAGGCCATAATTCA+AGG 0.492974 6_2:-68930539 Msa0925660:CDS
GGACTTGAGAAGCTTGGAAA+GGG 0.498462 6_2:-68929956 Msa0925660:CDS
AGCTTGGAAAGGGAGATTGG+AGG 0.502184 6_2:-68929946 Msa0925660:CDS
GGCCCAATAAGAAATGAACT+TGG 0.508375 6_2:+68928590 None:intergenic
ATCTGAAAGATAACCAATAG+AGG 0.510798 6_2:+68930335 None:intergenic
TTTATTAATTAGGAGTTCCA+TGG 0.511389 6_2:-68930009 Msa0925660:intron
GCCAGAGTTAGCTCAAGATC+AGG 0.513405 6_2:+68928653 None:intergenic
TGTGTTTATTGTCTCTTAGA+GGG 0.525698 6_2:+68930635 None:intergenic
GCCCAATAAGAAATGAACTT+GGG 0.527445 6_2:+68928591 None:intergenic
GCATGGCTTGCAACTTGTGT+TGG 0.527834 6_2:+68929890 None:intergenic
TCATAGTTCGTACACAAGAA+AGG 0.532269 6_2:-68930308 Msa0925660:CDS
GTAGGAGAAGAAGATGAAGT+AGG 0.532464 6_2:+68930399 None:intergenic
TCCAACCAATTAAATAGTGT+TGG 0.537821 6_2:-68930762 Msa0925660:five_prime_UTR
TTTAAGGTTAAGGTTTGTGT+CGG 0.548532 6_2:-68930595 Msa0925660:five_prime_UTR
GAATATGTTTAGGTTGGTAG+TGG 0.550713 6_2:-68928968 Msa0925660:intron
AAAGTCTTTAAATATGACAT+TGG 0.555274 6_2:-68928705 Msa0925660:CDS
TGGACATAGTTTATTCAATG+TGG 0.555850 6_2:+68930781 None:intergenic
TGCTCACATTGTGGAAACAT+AGG 0.564462 6_2:-68930552 Msa0925660:CDS
ACCTGATCTTGAGCTAACTC+TGG 0.566325 6_2:-68928654 Msa0925660:CDS
ATCTTGAGCTAACTCTGGCA+GGG 0.569532 6_2:-68928649 Msa0925660:CDS
TGGACTTGAGAAGCTTGGAA+AGG 0.582509 6_2:-68929957 Msa0925660:CDS
GGCAGAAAGTGCTCACATTG+TGG 0.586228 6_2:-68930561 Msa0925660:CDS
CCCAATAAGAAATGAACTTG+GGG 0.590590 6_2:+68928592 None:intergenic
CTTGGAAAGGGAGATTGGAG+GGG 0.594996 6_2:-68929944 Msa0925660:CDS
CAAGCTTCTCAAGTCCAACA+AGG 0.608243 6_2:+68929963 None:intergenic
TGATGATCATGAAGTTGAGA+AGG 0.617743 6_2:-68928876 Msa0925660:CDS
TGATATATATGATGATTGAT+GGG 0.621044 6_2:+68930704 None:intergenic
GATCATGAAGTTGAGAAGGA+TGG 0.622469 6_2:-68928872 Msa0925660:CDS
TGATTGATGGGTGTGAGCGG+TGG 0.632800 6_2:+68930716 None:intergenic
ACGTTCAAGTCTCTTTGACA+TGG 0.636224 6_2:-68929822 Msa0925660:intron
AGGGTCCAATTCAAATAACA+TGG 0.651228 6_2:-68928630 Msa0925660:CDS
TGATGATTGATGGGTGTGAG+CGG 0.665275 6_2:+68930713 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTGTTGTTTTATTAATT+AGG - chr6_2:68929260-68929279 Msa0925660:intron 10.0%
!! AAAAAAAACTTATACAAAAT+GGG + chr6_2:68929696-68929715 None:intergenic 10.0%
!! AAAAAAAAACTTATACAAAA+TGG + chr6_2:68929697-68929716 None:intergenic 10.0%
!!! ATTTTGTTGTTTTATTAATT+AGG - chr6_2:68929260-68929279 Msa0925660:intron 10.0%
!! AAAAAAAACTTATACAAAAT+GGG + chr6_2:68929696-68929715 None:intergenic 10.0%
!! AAAAAAAAACTTATACAAAA+TGG + chr6_2:68929697-68929716 None:intergenic 10.0%
!! ATTATAAAAAGAAGATTCTT+TGG + chr6_2:68929867-68929886 None:intergenic 15.0%
!!! CTTTTTATAATTGTTGAATT+AGG - chr6_2:68929876-68929895 Msa0925660:CDS 15.0%
!!! ATGATGTTATTTTAGAATTT+GGG + chr6_2:68929907-68929926 None:intergenic 15.0%
!!! TATGATGTTATTTTAGAATT+TGG + chr6_2:68929908-68929927 None:intergenic 15.0%
!! TAACCTATCAAAAAATTATT+AGG - chr6_2:68929926-68929945 Msa0925660:CDS 15.0%
!! ATTATAAAAAGAAGATTCTT+TGG + chr6_2:68929867-68929886 None:intergenic 15.0%
!!! CTTTTTATAATTGTTGAATT+AGG - chr6_2:68929876-68929895 Msa0925660:CDS 15.0%
!!! ATGATGTTATTTTAGAATTT+GGG + chr6_2:68929907-68929926 None:intergenic 15.0%
!!! TATGATGTTATTTTAGAATT+TGG + chr6_2:68929908-68929927 None:intergenic 15.0%
!! TAACCTATCAAAAAATTATT+AGG - chr6_2:68929926-68929945 Msa0925660:CDS 15.0%
!! TGATATATATGATGATTGAT+GGG + chr6_2:68928578-68928597 None:intergenic 20.0%
!!! TATTCTAGTTTTTAAGGTTA+AGG - chr6_2:68928674-68928693 Msa0925660:CDS 20.0%
!!! ATCAAATTTTATCAAGACAT+CGG - chr6_2:68929041-68929060 Msa0925660:intron 20.0%
!! CTATGATGTATTTATTATGA+AGG - chr6_2:68929122-68929141 Msa0925660:intron 20.0%
!! TATGCATTTCATTGAATTTA+AGG + chr6_2:68929576-68929595 None:intergenic 20.0%
!! TTTGAATTTACTCATTTAGA+CGG + chr6_2:68929816-68929835 None:intergenic 20.0%
!!! TGACCTAATAATTTTTTGAT+AGG + chr6_2:68929932-68929951 None:intergenic 20.0%
!! AAATAAAAAACGTCAAATGT+CGG - chr6_2:68930132-68930151 Msa0925660:intron 20.0%
!! ATATTAAATGTCAGTTAAAG+TGG + chr6_2:68930179-68930198 None:intergenic 20.0%
!! TAAAATGCAAATATCCAAAT+TGG + chr6_2:68930261-68930280 None:intergenic 20.0%
!! TGATATTGTTGAATATGTTT+AGG - chr6_2:68930301-68930320 Msa0925660:CDS 20.0%
!! CATATCATAATAATCTGATT+TGG + chr6_2:68930471-68930490 None:intergenic 20.0%
!! ATATTTAAAGACTTTGTTAG+AGG + chr6_2:68930569-68930588 None:intergenic 20.0%
!! AAAGTCTTTAAATATGACAT+TGG - chr6_2:68930574-68930593 Msa0925660:exon 20.0%
!! TGATATATATGATGATTGAT+GGG + chr6_2:68928578-68928597 None:intergenic 20.0%
!!! TATTCTAGTTTTTAAGGTTA+AGG - chr6_2:68928674-68928693 Msa0925660:CDS 20.0%
!!! ATCAAATTTTATCAAGACAT+CGG - chr6_2:68929041-68929060 Msa0925660:intron 20.0%
!! CTATGATGTATTTATTATGA+AGG - chr6_2:68929122-68929141 Msa0925660:intron 20.0%
!! TATGCATTTCATTGAATTTA+AGG + chr6_2:68929576-68929595 None:intergenic 20.0%
!! TTTGAATTTACTCATTTAGA+CGG + chr6_2:68929816-68929835 None:intergenic 20.0%
!!! TGACCTAATAATTTTTTGAT+AGG + chr6_2:68929932-68929951 None:intergenic 20.0%
!! AAATAAAAAACGTCAAATGT+CGG - chr6_2:68930132-68930151 Msa0925660:intron 20.0%
!! ATATTAAATGTCAGTTAAAG+TGG + chr6_2:68930179-68930198 None:intergenic 20.0%
!! TAAAATGCAAATATCCAAAT+TGG + chr6_2:68930261-68930280 None:intergenic 20.0%
!! TGATATTGTTGAATATGTTT+AGG - chr6_2:68930301-68930320 Msa0925660:CDS 20.0%
!! CATATCATAATAATCTGATT+TGG + chr6_2:68930471-68930490 None:intergenic 20.0%
!! ATATTTAAAGACTTTGTTAG+AGG + chr6_2:68930569-68930588 None:intergenic 20.0%
!! AAAGTCTTTAAATATGACAT+TGG - chr6_2:68930574-68930593 Msa0925660:exon 20.0%
! GTGATATATATGATGATTGA+TGG + chr6_2:68928579-68928598 None:intergenic 25.0%
!!! GTTTGGTATTCTAGTTTTTA+AGG - chr6_2:68928668-68928687 Msa0925660:CDS 25.0%
! AATTACAAGTCCTTGAATTA+TGG + chr6_2:68928753-68928772 None:intergenic 25.0%
! TTTATTAATTAGGAGTTCCA+TGG - chr6_2:68929270-68929289 Msa0925660:intron 25.0%
!! GAATAAAATACTTTTGAGCA+TGG + chr6_2:68929409-68929428 None:intergenic 25.0%
! TCTATAAAAGAGTTCATATG+TGG - chr6_2:68929645-68929664 Msa0925660:intron 25.0%
! CTATAAAAGAGTTCATATGT+GGG - chr6_2:68929646-68929665 Msa0925660:intron 25.0%
! AAAAACTTATACAAAATGGG+CGG + chr6_2:68929693-68929712 None:intergenic 25.0%
! ATGTTCAATCTTAGACATAA+AGG - chr6_2:68930037-68930056 Msa0925660:intron 25.0%
! ATTGTTGAATATGTTTAGGT+TGG - chr6_2:68930305-68930324 Msa0925660:CDS 25.0%
!! TTGTTCCATGTTATTTGAAT+TGG + chr6_2:68930657-68930676 None:intergenic 25.0%
! TTGAAAAGTTGCATACATAA+AGG + chr6_2:68930767-68930786 None:intergenic 25.0%
! GTGATATATATGATGATTGA+TGG + chr6_2:68928579-68928598 None:intergenic 25.0%
!!! GTTTGGTATTCTAGTTTTTA+AGG - chr6_2:68928668-68928687 Msa0925660:CDS 25.0%
! AATTACAAGTCCTTGAATTA+TGG + chr6_2:68928753-68928772 None:intergenic 25.0%
! TTTATTAATTAGGAGTTCCA+TGG - chr6_2:68929270-68929289 Msa0925660:intron 25.0%
!! GAATAAAATACTTTTGAGCA+TGG + chr6_2:68929409-68929428 None:intergenic 25.0%
! TCTATAAAAGAGTTCATATG+TGG - chr6_2:68929645-68929664 Msa0925660:intron 25.0%
! CTATAAAAGAGTTCATATGT+GGG - chr6_2:68929646-68929665 Msa0925660:intron 25.0%
! AAAAACTTATACAAAATGGG+CGG + chr6_2:68929693-68929712 None:intergenic 25.0%
! ATGTTCAATCTTAGACATAA+AGG - chr6_2:68930037-68930056 Msa0925660:intron 25.0%
! ATTGTTGAATATGTTTAGGT+TGG - chr6_2:68930305-68930324 Msa0925660:CDS 25.0%
!! TTGTTCCATGTTATTTGAAT+TGG + chr6_2:68930657-68930676 None:intergenic 25.0%
! TTGAAAAGTTGCATACATAA+AGG + chr6_2:68930767-68930786 None:intergenic 25.0%
TGGACATAGTTTATTCAATG+TGG + chr6_2:68928501-68928520 None:intergenic 30.0%
TCCAACCAATTAAATAGTGT+TGG - chr6_2:68928517-68928536 Msa0925660:three_prime_UTR 30.0%
ACCAACACTATTTAATTGGT+TGG + chr6_2:68928521-68928540 None:intergenic 30.0%
! ACACATAAGTTCTTTTGTGT+TGG - chr6_2:68928602-68928621 Msa0925660:CDS 30.0%
TGTGTTTATTGTCTCTTAGA+GGG + chr6_2:68928647-68928666 None:intergenic 30.0%
TTGTGTTTATTGTCTCTTAG+AGG + chr6_2:68928648-68928667 None:intergenic 30.0%
AGAGACAATAAACACAAGTT+TGG - chr6_2:68928651-68928670 Msa0925660:CDS 30.0%
! TTTAAGGTTAAGGTTTGTGT+CGG - chr6_2:68928684-68928703 Msa0925660:CDS 30.0%
!! TTTGTGTCGGTTCAAAAAAT+GGG - chr6_2:68928697-68928716 Msa0925660:CDS 30.0%
AGGACTTGTAATTCATTAAG+AGG - chr6_2:68928760-68928779 Msa0925660:CDS 30.0%
! TTTGTTGGAGTTAGACTTTT+TGG - chr6_2:68928793-68928812 Msa0925660:CDS 30.0%
!! CATGCTAAAACTCTTTTTCA+TGG + chr6_2:68928851-68928870 None:intergenic 30.0%
!! CATGAAAAAGAGTTTTAGCA+TGG - chr6_2:68928849-68928868 Msa0925660:CDS 30.0%
GGATAATCTTGAAGAAGAAA+AGG + chr6_2:68928911-68928930 None:intergenic 30.0%
ATCTGAAAGATAACCAATAG+AGG + chr6_2:68928947-68928966 None:intergenic 30.0%
! TCTATTGGTTATCTTTCAGA+TGG - chr6_2:68928946-68928965 Msa0925660:CDS 30.0%
!!! TCATCCATTTTGAAGTATCT+AGG - chr6_2:68929090-68929109 Msa0925660:intron 30.0%
! TCATTGAATTTAAGGTCATG+AGG + chr6_2:68929568-68929587 None:intergenic 30.0%
AAAACTTATACAAAATGGGC+GGG + chr6_2:68929692-68929711 None:intergenic 30.0%
!! GTGCTTTTTAAGAAATCAGA+GGG + chr6_2:68929729-68929748 None:intergenic 30.0%
!! TGTGCTTTTTAAGAAATCAG+AGG + chr6_2:68929730-68929749 None:intergenic 30.0%
!! TGAGTGGTTTTTAAGAATCA+TGG - chr6_2:68929838-68929857 Msa0925660:CDS 30.0%
!!! TTGCTTTTTGAGCTGTTATT+TGG + chr6_2:68930359-68930378 None:intergenic 30.0%
GCTAATTAAGTCACACTAAT+TGG + chr6_2:68930713-68930732 None:intergenic 30.0%
TGGACATAGTTTATTCAATG+TGG + chr6_2:68928501-68928520 None:intergenic 30.0%
TCCAACCAATTAAATAGTGT+TGG - chr6_2:68928517-68928536 Msa0925660:three_prime_UTR 30.0%
ACCAACACTATTTAATTGGT+TGG + chr6_2:68928521-68928540 None:intergenic 30.0%
! ACACATAAGTTCTTTTGTGT+TGG - chr6_2:68928602-68928621 Msa0925660:CDS 30.0%
TGTGTTTATTGTCTCTTAGA+GGG + chr6_2:68928647-68928666 None:intergenic 30.0%
TTGTGTTTATTGTCTCTTAG+AGG + chr6_2:68928648-68928667 None:intergenic 30.0%
AGAGACAATAAACACAAGTT+TGG - chr6_2:68928651-68928670 Msa0925660:CDS 30.0%
! TTTAAGGTTAAGGTTTGTGT+CGG - chr6_2:68928684-68928703 Msa0925660:CDS 30.0%
!! TTTGTGTCGGTTCAAAAAAT+GGG - chr6_2:68928697-68928716 Msa0925660:CDS 30.0%
AGGACTTGTAATTCATTAAG+AGG - chr6_2:68928760-68928779 Msa0925660:CDS 30.0%
! TTTGTTGGAGTTAGACTTTT+TGG - chr6_2:68928793-68928812 Msa0925660:CDS 30.0%
!! CATGCTAAAACTCTTTTTCA+TGG + chr6_2:68928851-68928870 None:intergenic 30.0%
!! CATGAAAAAGAGTTTTAGCA+TGG - chr6_2:68928849-68928868 Msa0925660:CDS 30.0%
GGATAATCTTGAAGAAGAAA+AGG + chr6_2:68928911-68928930 None:intergenic 30.0%
ATCTGAAAGATAACCAATAG+AGG + chr6_2:68928947-68928966 None:intergenic 30.0%
! TCTATTGGTTATCTTTCAGA+TGG - chr6_2:68928946-68928965 Msa0925660:CDS 30.0%
!!! TCATCCATTTTGAAGTATCT+AGG - chr6_2:68929090-68929109 Msa0925660:intron 30.0%
! TCATTGAATTTAAGGTCATG+AGG + chr6_2:68929568-68929587 None:intergenic 30.0%
AAAACTTATACAAAATGGGC+GGG + chr6_2:68929692-68929711 None:intergenic 30.0%
!! GTGCTTTTTAAGAAATCAGA+GGG + chr6_2:68929729-68929748 None:intergenic 30.0%
!! TGTGCTTTTTAAGAAATCAG+AGG + chr6_2:68929730-68929749 None:intergenic 30.0%
!! TGAGTGGTTTTTAAGAATCA+TGG - chr6_2:68929838-68929857 Msa0925660:CDS 30.0%
!!! TTGCTTTTTGAGCTGTTATT+TGG + chr6_2:68930359-68930378 None:intergenic 30.0%
GCTAATTAAGTCACACTAAT+TGG + chr6_2:68930713-68930732 None:intergenic 30.0%
GCACACCAACACTATTTAAT+TGG + chr6_2:68928525-68928544 None:intergenic 35.0%
CACAAAAGAACTTATGTGTC+TGG + chr6_2:68928601-68928620 None:intergenic 35.0%
!! GTTTGTGTCGGTTCAAAAAA+TGG - chr6_2:68928696-68928715 Msa0925660:CDS 35.0%
GAAACATAGGCCATAATTCA+AGG - chr6_2:68928740-68928759 Msa0925660:CDS 35.0%
!!! AGAGGAAGTAGTAGTTTTGT+TGG - chr6_2:68928778-68928797 Msa0925660:CDS 35.0%
GAAGAAGAAAAGGAAGAAGT+AGG + chr6_2:68928901-68928920 None:intergenic 35.0%
AATAGAGGCTCTATCATCAA+TGG + chr6_2:68928932-68928951 None:intergenic 35.0%
! ATTGATGATAGAGCCTCTAT+TGG - chr6_2:68928931-68928950 Msa0925660:CDS 35.0%
TTTCTTGTGTACGAACTATG+AGG + chr6_2:68928972-68928991 None:intergenic 35.0%
TCATAGTTCGTACACAAGAA+AGG - chr6_2:68928971-68928990 Msa0925660:intron 35.0%
CGTACACAAGAAAGGAAAAA+AGG - chr6_2:68928979-68928998 Msa0925660:intron 35.0%
GAAGCCTAGATACTTCAAAA+TGG + chr6_2:68929097-68929116 None:intergenic 35.0%
GAGCATAGAAAATTCCTTGT+TGG - chr6_2:68929302-68929321 Msa0925660:intron 35.0%
ACGCGTTTGGATGTATAATT+CGG - chr6_2:68929774-68929793 Msa0925660:intron 35.0%
TGAGTAAATTCAAACGTGAG+TGG - chr6_2:68929822-68929841 Msa0925660:intron 35.0%
!! GTGGTTTTTAAGAATCATGG+TGG - chr6_2:68929841-68929860 Msa0925660:CDS 35.0%
TCCTTCTCAAAAGACCAATT+TGG - chr6_2:68930244-68930263 Msa0925660:intron 35.0%
!!! TCCAAATTGGTCTTTTGAGA+AGG + chr6_2:68930248-68930267 None:intergenic 35.0%
!! GAATATGTTTAGGTTGGTAG+TGG - chr6_2:68930311-68930330 Msa0925660:CDS 35.0%
TGATGATCATGAAGTTGAGA+AGG - chr6_2:68930403-68930422 Msa0925660:CDS 35.0%
AACATAAAGATGTGCCAGAT+TGG - chr6_2:68930537-68930556 Msa0925660:CDS 35.0%
GCTGCTACATTAGAAGAATT+TGG + chr6_2:68930605-68930624 None:intergenic 35.0%
AGGGTCCAATTCAAATAACA+TGG - chr6_2:68930649-68930668 Msa0925660:five_prime_UTR 35.0%
TCCCCAAGTTCATTTCTTAT+TGG - chr6_2:68930686-68930705 Msa0925660:five_prime_UTR 35.0%
CCCAATAAGAAATGAACTTG+GGG + chr6_2:68930690-68930709 None:intergenic 35.0%
CCCCAAGTTCATTTCTTATT+GGG - chr6_2:68930687-68930706 Msa0925660:five_prime_UTR 35.0%
GCCCAATAAGAAATGAACTT+GGG + chr6_2:68930691-68930710 None:intergenic 35.0%
GCACACCAACACTATTTAAT+TGG + chr6_2:68928525-68928544 None:intergenic 35.0%
CACAAAAGAACTTATGTGTC+TGG + chr6_2:68928601-68928620 None:intergenic 35.0%
!! GTTTGTGTCGGTTCAAAAAA+TGG - chr6_2:68928696-68928715 Msa0925660:CDS 35.0%
GAAACATAGGCCATAATTCA+AGG - chr6_2:68928740-68928759 Msa0925660:CDS 35.0%
!!! AGAGGAAGTAGTAGTTTTGT+TGG - chr6_2:68928778-68928797 Msa0925660:CDS 35.0%
GAAGAAGAAAAGGAAGAAGT+AGG + chr6_2:68928901-68928920 None:intergenic 35.0%
AATAGAGGCTCTATCATCAA+TGG + chr6_2:68928932-68928951 None:intergenic 35.0%
! ATTGATGATAGAGCCTCTAT+TGG - chr6_2:68928931-68928950 Msa0925660:CDS 35.0%
TTTCTTGTGTACGAACTATG+AGG + chr6_2:68928972-68928991 None:intergenic 35.0%
TCATAGTTCGTACACAAGAA+AGG - chr6_2:68928971-68928990 Msa0925660:intron 35.0%
CGTACACAAGAAAGGAAAAA+AGG - chr6_2:68928979-68928998 Msa0925660:intron 35.0%
GAAGCCTAGATACTTCAAAA+TGG + chr6_2:68929097-68929116 None:intergenic 35.0%
GAGCATAGAAAATTCCTTGT+TGG - chr6_2:68929302-68929321 Msa0925660:intron 35.0%
ACGCGTTTGGATGTATAATT+CGG - chr6_2:68929774-68929793 Msa0925660:intron 35.0%
TGAGTAAATTCAAACGTGAG+TGG - chr6_2:68929822-68929841 Msa0925660:intron 35.0%
!! GTGGTTTTTAAGAATCATGG+TGG - chr6_2:68929841-68929860 Msa0925660:CDS 35.0%
TCCTTCTCAAAAGACCAATT+TGG - chr6_2:68930244-68930263 Msa0925660:intron 35.0%
!!! TCCAAATTGGTCTTTTGAGA+AGG + chr6_2:68930248-68930267 None:intergenic 35.0%
!! GAATATGTTTAGGTTGGTAG+TGG - chr6_2:68930311-68930330 Msa0925660:CDS 35.0%
TGATGATCATGAAGTTGAGA+AGG - chr6_2:68930403-68930422 Msa0925660:CDS 35.0%
AACATAAAGATGTGCCAGAT+TGG - chr6_2:68930537-68930556 Msa0925660:CDS 35.0%
GCTGCTACATTAGAAGAATT+TGG + chr6_2:68930605-68930624 None:intergenic 35.0%
AGGGTCCAATTCAAATAACA+TGG - chr6_2:68930649-68930668 Msa0925660:five_prime_UTR 35.0%
TCCCCAAGTTCATTTCTTAT+TGG - chr6_2:68930686-68930705 Msa0925660:five_prime_UTR 35.0%
CCCAATAAGAAATGAACTTG+GGG + chr6_2:68930690-68930709 None:intergenic 35.0%
CCCCAAGTTCATTTCTTATT+GGG - chr6_2:68930687-68930706 Msa0925660:five_prime_UTR 35.0%
GCCCAATAAGAAATGAACTT+GGG + chr6_2:68930691-68930710 None:intergenic 35.0%
GCGGTGGAATTTGTGAAAAT+TGG + chr6_2:68928550-68928569 None:intergenic 40.0%
TGCTCACATTGTGGAAACAT+AGG - chr6_2:68928727-68928746 Msa0925660:CDS 40.0%
GTAGGAGAAGAAGATGAAGT+AGG + chr6_2:68928883-68928902 None:intergenic 40.0%
! TTTTATCAAGACATCGGTGC+AGG - chr6_2:68929047-68929066 Msa0925660:intron 40.0%
ACGTCTCTTCTTCTTGTTCA+AGG + chr6_2:68929441-68929460 None:intergenic 40.0%
ACGTTCAAGTCTCTTTGACA+TGG - chr6_2:68929457-68929476 Msa0925660:intron 40.0%
! AAAAGCACACCACTTCGAAT+TGG - chr6_2:68929741-68929760 Msa0925660:intron 40.0%
CAGTTAAAGTGGTCTCGATA+AGG + chr6_2:68930168-68930187 None:intergenic 40.0%
AACAGCTCAAAAAGCAAGTC+AGG - chr6_2:68930362-68930381 Msa0925660:CDS 40.0%
GATCATGAAGTTGAGAAGGA+TGG - chr6_2:68930407-68930426 Msa0925660:CDS 40.0%
GGCCCAATAAGAAATGAACT+TGG + chr6_2:68930692-68930711 None:intergenic 40.0%
GCGGTGGAATTTGTGAAAAT+TGG + chr6_2:68928550-68928569 None:intergenic 40.0%
TGCTCACATTGTGGAAACAT+AGG - chr6_2:68928727-68928746 Msa0925660:CDS 40.0%
GTAGGAGAAGAAGATGAAGT+AGG + chr6_2:68928883-68928902 None:intergenic 40.0%
! TTTTATCAAGACATCGGTGC+AGG - chr6_2:68929047-68929066 Msa0925660:intron 40.0%
ACGTCTCTTCTTCTTGTTCA+AGG + chr6_2:68929441-68929460 None:intergenic 40.0%
ACGTTCAAGTCTCTTTGACA+TGG - chr6_2:68929457-68929476 Msa0925660:intron 40.0%
! AAAAGCACACCACTTCGAAT+TGG - chr6_2:68929741-68929760 Msa0925660:intron 40.0%
CAGTTAAAGTGGTCTCGATA+AGG + chr6_2:68930168-68930187 None:intergenic 40.0%
AACAGCTCAAAAAGCAAGTC+AGG - chr6_2:68930362-68930381 Msa0925660:CDS 40.0%
GATCATGAAGTTGAGAAGGA+TGG - chr6_2:68930407-68930426 Msa0925660:CDS 40.0%
GGCCCAATAAGAAATGAACT+TGG + chr6_2:68930692-68930711 None:intergenic 40.0%
! TGATGATTGATGGGTGTGAG+CGG + chr6_2:68928569-68928588 None:intergenic 45.0%
CTATGCTCTTCTTCTGTCCA+TGG + chr6_2:68929290-68929309 None:intergenic 45.0%
CAAGCTTCTCAAGTCCAACA+AGG + chr6_2:68929319-68929338 None:intergenic 45.0%
CTTGTTGGACTTGAGAAGCT+TGG - chr6_2:68929317-68929336 Msa0925660:intron 45.0%
TGGACTTGAGAAGCTTGGAA+AGG - chr6_2:68929322-68929341 Msa0925660:intron 45.0%
GGACTTGAGAAGCTTGGAAA+GGG - chr6_2:68929323-68929342 Msa0925660:intron 45.0%
AGAAGCTTGGAAAGGGAGAT+TGG - chr6_2:68929330-68929349 Msa0925660:intron 45.0%
CACACCACTTCGAATTGGAA+AGG - chr6_2:68929746-68929765 Msa0925660:intron 45.0%
GTTAGAGGATGTAGCCAATC+TGG + chr6_2:68930554-68930573 None:intergenic 45.0%
ACCTGATCTTGAGCTAACTC+TGG - chr6_2:68930625-68930644 Msa0925660:five_prime_UTR 45.0%
ATCTTGAGCTAACTCTGGCA+GGG - chr6_2:68930630-68930649 Msa0925660:five_prime_UTR 45.0%
! TGATGATTGATGGGTGTGAG+CGG + chr6_2:68928569-68928588 None:intergenic 45.0%
CTATGCTCTTCTTCTGTCCA+TGG + chr6_2:68929290-68929309 None:intergenic 45.0%
CAAGCTTCTCAAGTCCAACA+AGG + chr6_2:68929319-68929338 None:intergenic 45.0%
CTTGTTGGACTTGAGAAGCT+TGG - chr6_2:68929317-68929336 Msa0925660:intron 45.0%
TGGACTTGAGAAGCTTGGAA+AGG - chr6_2:68929322-68929341 Msa0925660:intron 45.0%
GGACTTGAGAAGCTTGGAAA+GGG - chr6_2:68929323-68929342 Msa0925660:intron 45.0%
AGAAGCTTGGAAAGGGAGAT+TGG - chr6_2:68929330-68929349 Msa0925660:intron 45.0%
CACACCACTTCGAATTGGAA+AGG - chr6_2:68929746-68929765 Msa0925660:intron 45.0%
GTTAGAGGATGTAGCCAATC+TGG + chr6_2:68930554-68930573 None:intergenic 45.0%
ACCTGATCTTGAGCTAACTC+TGG - chr6_2:68930625-68930644 Msa0925660:five_prime_UTR 45.0%
ATCTTGAGCTAACTCTGGCA+GGG - chr6_2:68930630-68930649 Msa0925660:five_prime_UTR 45.0%
!!! TTTTATATATAAATTAAGAA+AGG + chr6_2:68929523-68929542 None:intergenic 5.0%
!!! TTTTATATATAAATTAAGAA+AGG + chr6_2:68929523-68929542 None:intergenic 5.0%
!! GGCAGAAAGTGCTCACATTG+TGG - chr6_2:68928718-68928737 Msa0925660:CDS 50.0%
AGCTTGGAAAGGGAGATTGG+AGG - chr6_2:68929333-68929352 Msa0925660:intron 50.0%
GCTTGGAAAGGGAGATTGGA+GGG - chr6_2:68929334-68929353 Msa0925660:intron 50.0%
CTTGGAAAGGGAGATTGGAG+GGG - chr6_2:68929335-68929354 Msa0925660:intron 50.0%
! GCATGGCTTGCAACTTGTGT+TGG + chr6_2:68929392-68929411 None:intergenic 50.0%
CGTGCCTTTCCAATTCGAAG+TGG + chr6_2:68929753-68929772 None:intergenic 50.0%
GCCAGAGTTAGCTCAAGATC+AGG + chr6_2:68930629-68930648 None:intergenic 50.0%
GATCTTGAGCTAACTCTGGC+AGG - chr6_2:68930629-68930648 Msa0925660:five_prime_UTR 50.0%
!! GGCAGAAAGTGCTCACATTG+TGG - chr6_2:68928718-68928737 Msa0925660:CDS 50.0%
AGCTTGGAAAGGGAGATTGG+AGG - chr6_2:68929333-68929352 Msa0925660:intron 50.0%
GCTTGGAAAGGGAGATTGGA+GGG - chr6_2:68929334-68929353 Msa0925660:intron 50.0%
CTTGGAAAGGGAGATTGGAG+GGG - chr6_2:68929335-68929354 Msa0925660:intron 50.0%
! GCATGGCTTGCAACTTGTGT+TGG + chr6_2:68929392-68929411 None:intergenic 50.0%
CGTGCCTTTCCAATTCGAAG+TGG + chr6_2:68929753-68929772 None:intergenic 50.0%
GCCAGAGTTAGCTCAAGATC+AGG + chr6_2:68930629-68930648 None:intergenic 50.0%
GATCTTGAGCTAACTCTGGC+AGG - chr6_2:68930629-68930648 Msa0925660:five_prime_UTR 50.0%
! TGATTGATGGGTGTGAGCGG+TGG + chr6_2:68928566-68928585 None:intergenic 55.0%
ACATCGGTGCAGGATACGCA+GGG - chr6_2:68929057-68929076 Msa0925660:intron 55.0%
GTGCAGGATACGCAGGGATA+CGG - chr6_2:68929063-68929082 Msa0925660:intron 55.0%
! TGGAAAGGCACGAACGCGTT+TGG - chr6_2:68929761-68929780 Msa0925660:intron 55.0%
! TGATTGATGGGTGTGAGCGG+TGG + chr6_2:68928566-68928585 None:intergenic 55.0%
ACATCGGTGCAGGATACGCA+GGG - chr6_2:68929057-68929076 Msa0925660:intron 55.0%
GTGCAGGATACGCAGGGATA+CGG - chr6_2:68929063-68929082 Msa0925660:intron 55.0%
! TGGAAAGGCACGAACGCGTT+TGG - chr6_2:68929761-68929780 Msa0925660:intron 55.0%
GACATCGGTGCAGGATACGC+AGG - chr6_2:68929056-68929075 Msa0925660:intron 60.0%
GACATCGGTGCAGGATACGC+AGG - chr6_2:68929056-68929075 Msa0925660:intron 60.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 68928474 68930827 68928474 ID=Msa0925660;Name=Msa0925660
chr6_2 mRNA 68928474 68930827 68928474 ID=Msa0925660-mRNA-1;Parent=Msa0925660;Name=Msa0925660-mRNA-1;_AED=0.20;_eAED=0.20;_QI=241|1|1|1|1|1|3|100|295
chr6_2 exon 68928474 68928978 68928474 ID=Msa0925660-mRNA-1:exon:11076;Parent=Msa0925660-mRNA-1
chr6_2 exon 68929823 68930019 68929823 ID=Msa0925660-mRNA-1:exon:11075;Parent=Msa0925660-mRNA-1
chr6_2 exon 68930301 68930827 68930301 ID=Msa0925660-mRNA-1:exon:11074;Parent=Msa0925660-mRNA-1
chr6_2 five_prime_UTR 68930587 68930827 68930587 ID=Msa0925660-mRNA-1:five_prime_utr;Parent=Msa0925660-mRNA-1
chr6_2 CDS 68930301 68930586 68930301 ID=Msa0925660-mRNA-1:cds;Parent=Msa0925660-mRNA-1
chr6_2 CDS 68929823 68930019 68929823 ID=Msa0925660-mRNA-1:cds;Parent=Msa0925660-mRNA-1
chr6_2 CDS 68928574 68928978 68928574 ID=Msa0925660-mRNA-1:cds;Parent=Msa0925660-mRNA-1
chr6_2 three_prime_UTR 68928474 68928573 68928474 ID=Msa0925660-mRNA-1:three_prime_utr;Parent=Msa0925660-mRNA-1
Gene Sequence

>Msa0925660

ATGGGCAGAAAGTGCTCACATTGTGGAAACATAGGCCATAATTCAAGGACTTGTAATTCATTAAGAGGAAGTAGTAGTTTTGTTGGAGTTAGACTTTTTGGTGTTCAACTTGATTTATCTTCTTCTTGTGTTTCCATGAAAAAGAGTTTTAGCATGGATTCTTTTCCTACTTCATCTTCTTCTCCTACTTCTTCCTTTTCTTCTTCAAGATTATCCATTGATGATAGAGCCTCTATTGGTTATCTTTCAGATGGCCTCATAGTTCGTACACAAGAAAGGAAAAAAGGAGTTCCATGGACAGAAGAAGAGCATAGAAAATTCCTTGTTGGACTTGAGAAGCTTGGAAAGGGAGATTGGAGGGGTATCTCTAGAAACTATGTGACTACAAGAACACCAACACAAGTTGCAAGCCATGCTCAAAAGTATTTTATTCGACTTGCTACCTTGAACAAGAAGAAGAGACGTTCAAGTCTCTTTGACATGGTTGGTAGTGGCAAGACAAACAAAGCAGTTGATCCAAATAACAGCTCAAAAAGCAAGTCAGGTGATTCAGTTTGTAGACATGATGATCATGAAGTTGAGAAGGATGGAACTTTGTCACTACTAATAAACTCACTTCAACAACAAACCAAATCAGATTATTATGATATGCAAAAAATTGAAGATGTTGAAGTTGAAGATGATTCTGAAGAAGCAGAACATAAAGATGTGCCAGATTGGCTACATCCTCTAACAAAGTCTTTAAATATGACATTGGTGATACCAAATTCTTCTAATGTAGCAGCACCTGATCTTGAGCTAACTCTGGCAGGGTCCAATTCAAATAACATGGAACAAGATAAAACATCCCCAAGTTCATTTCTTATTGGGCCAATTAGTGTGACTTAA
Protein sequence

>Msa0925660

MGRKCSHCGNIGHNSRTCNSLRGSSSFVGVRLFGVQLDLSSSCVSMKKSFSMDSFPTSSSSPTSSFSSSRLSIDDRASIGYLSDGLIVRTQERKKGVPWTEEEHRKFLVGLEKLGKGDWRGISRNYVTTRTPTQVASHAQKYFIRLATLNKKKRRSSLFDMVGSGKTNKAVDPNNSSKSKSGDSVCRHDDHEVEKDGTLSLLINSLQQQTKSDYYDMQKIEDVEVEDDSEEAEHKDVPDWLHPLTKSLNMTLVIPNSSNVAAPDLELTLAGSNSNNMEQDKTSPSSFLIGPISVT*