AlfalfaGEDB | Msa1315110

M. sativa cultivar zhongmu-4 / Msa1315110

KEGG: K09512
GO: GO:0003674 GO:0005488 GO:0005515 GO:0051087
PFAM: PF00226.34

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1315110	XP_003627374.1	97.458	236	5	1	1	236	1	235	2.24e-166	468

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1315110	sp\|Q4R7Y5\|DNJB6_MACFA	49.275	69	30	1	10	78	3	66	2.03e-15	76.3

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1315110	G7L8C9	97.458	236	5	1	1	236	1	235	1.07e-166	468

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0006040	Msa1315110	0.883849	3.167371e-71	-8.615850e-47
Msa0013050	Msa1315110	-0.809498	1.836488e-50	-8.615850e-47
Msa0032610	Msa1315110	0.835657	1.477243e-56	-8.615850e-47
Msa0036160	Msa1315110	0.848846	4.724868e-60	-8.615850e-47
Msa0042980	Msa1315110	-0.827975	1.162075e-54	-8.615850e-47
Msa0054750	Msa1315110	0.877302	7.007469e-69	-8.615850e-47
Msa0056030	Msa1315110	0.808691	2.734689e-50	-8.615850e-47
Msa0111310	Msa1315110	0.803504	3.378021e-49	-8.615850e-47
Msa0615270	Msa1315110	-0.820215	7.717453e-53	-8.615850e-47
Msa0629650	Msa1315110	0.831077	2.047815e-55	-8.615850e-47
Msa0650730	Msa1315110	0.808222	3.443281e-50	-8.615850e-47
Msa0654010	Msa1315110	0.821007	5.076529e-53	-8.615850e-47
Msa0661190	Msa1315110	-0.811001	8.708605e-51	-8.615850e-47
Msa0661210	Msa1315110	0.878590	2.483459e-69	-8.615850e-47
Msa0671740	Msa1315110	0.808302	3.310661e-50	-8.615850e-47
Msa0684850	Msa1315110	0.820608	6.270210e-53	-8.615850e-47
Msa0698310	Msa1315110	-0.805490	1.301683e-49	-8.615850e-47
Msa0709670	Msa1315110	0.815115	1.090610e-51	-8.615850e-47
Msa0722910	Msa1315110	0.812091	5.046948e-51	-8.615850e-47
Msa0722920	Msa1315110	0.821828	3.280702e-53	-8.615850e-47
Msa0723130	Msa1315110	0.842820	2.047157e-58	-8.615850e-47
Msa0739680	Msa1315110	0.813995	1.930015e-51	-8.615850e-47
Msa0755080	Msa1315110	0.859092	5.253246e-63	-8.615850e-47
Msa0782470	Msa1315110	0.810739	9.923676e-51	-8.615850e-47
Msa0782490	Msa1315110	0.800994	1.109765e-48	-8.615850e-47
Msa0791820	Msa1315110	0.815707	8.052126e-52	-8.615850e-47
Msa0795920	Msa1315110	0.842901	1.947827e-58	-8.615850e-47
Msa0796950	Msa1315110	0.827959	1.172721e-54	-8.615850e-47
Msa0797970	Msa1315110	0.867428	1.378229e-65	-8.615850e-47
Msa0801360	Msa1315110	0.807352	5.271567e-50	-8.615850e-47
Msa0834450	Msa1315110	0.859587	3.731574e-63	-8.615850e-47
Msa0834460	Msa1315110	0.800239	1.582043e-48	-8.615850e-47
Msa0839830	Msa1315110	0.806030	1.002354e-49	-8.615850e-47
Msa0125640	Msa1315110	-0.825062	5.753438e-54	-8.615850e-47
Msa0136890	Msa1315110	-0.822311	2.535221e-53	-8.615850e-47
Msa0148120	Msa1315110	0.856225	3.714787e-62	-8.615850e-47
Msa0170110	Msa1315110	-0.806860	6.700631e-50	-8.615850e-47
Msa0172780	Msa1315110	0.821095	4.844062e-53	-8.615850e-47
Msa0177300	Msa1315110	0.810232	1.276665e-50	-8.615850e-47
Msa0177340	Msa1315110	0.804986	1.659597e-49	-8.615850e-47
Msa0178860	Msa1315110	-0.803298	3.726631e-49	-8.615850e-47
Msa0197180	Msa1315110	0.836261	1.037852e-56	-8.615850e-47
Msa0221960	Msa1315110	-0.822839	1.911230e-53	-8.615850e-47
Msa0229490	Msa1315110	-0.809028	2.316572e-50	-8.615850e-47
Msa1234690	Msa1315110	0.977061	4.921096e-143	-8.615850e-47
Msa1234730	Msa1315110	0.995474	1.258567e-216	-8.615850e-47
Msa1258380	Msa1315110	-0.801459	8.914248e-49	-8.615850e-47
Msa1260130	Msa1315110	0.819347	1.218305e-52	-8.615850e-47
Msa1262920	Msa1315110	0.811725	6.064108e-51	-8.615850e-47
Msa1267060	Msa1315110	0.802378	5.772041e-49	-8.615850e-47
Msa1282920	Msa1315110	0.812028	5.209004e-51	-8.615850e-47
Msa1300050	Msa1315110	0.803511	3.366773e-49	-8.615850e-47
Msa1301650	Msa1315110	0.854286	1.362046e-61	-8.615850e-47
Msa1302100	Msa1315110	0.832333	1.003812e-55	-8.615850e-47
Msa1307740	Msa1315110	0.839411	1.610756e-57	-8.615850e-47
Msa1315110	Msa1322190	0.815847	7.494099e-52	-8.615850e-47
Msa1315110	Msa1342100	0.807681	4.489365e-50	-8.615850e-47
Msa1315110	Msa1359630	0.880706	4.399485e-70	-8.615850e-47
Msa1315110	Msa1362840	0.811972	5.358212e-51	-8.615850e-47
Msa1315110	Msa1388860	0.818024	2.432375e-52	-8.615850e-47
Msa1315110	Msa1401530	0.801547	8.553514e-49	-8.615850e-47
Msa1315110	Msa1403340	0.825569	4.363604e-54	-8.615850e-47
Msa1315110	Msa1419970	0.840258	9.690137e-58	-8.615850e-47
Msa1315110	Msa1427830	0.800869	1.177087e-48	-8.615850e-47
Msa1315110	Msa1437910	0.805560	1.258727e-49	-8.615850e-47
Msa1315110	Msa1444250	-0.807233	5.587541e-50	-8.615850e-47
Msa1315110	Msa1459760	-0.839558	1.474572e-57	-8.615850e-47
Msa1315110	Msa1465960	0.834691	2.589508e-56	-8.615850e-47
Msa0369720	Msa1315110	-0.812958	3.261977e-51	-8.615850e-47
Msa0379410	Msa1315110	0.842673	2.239893e-58	-8.615850e-47
Msa0380040	Msa1315110	0.813142	2.972735e-51	-8.615850e-47
Msa0381410	Msa1315110	0.806509	7.946749e-50	-8.615850e-47
Msa0389810	Msa1315110	0.840280	9.562179e-58	-8.615850e-47
Msa0394340	Msa1315110	0.838636	2.556952e-57	-8.615850e-47
Msa0429290	Msa1315110	0.811703	6.130929e-51	-8.615850e-47
Msa0442200	Msa1315110	0.827895	1.215143e-54	-8.615850e-47
Msa0457510	Msa1315110	0.833012	6.808388e-56	-8.615850e-47
Msa0463860	Msa1315110	0.800811	1.209883e-48	-8.615850e-47
Msa0468130	Msa1315110	0.846411	2.209656e-59	-8.615850e-47
Msa0475240	Msa1315110	0.827329	1.661115e-54	-8.615850e-47
Msa0475580	Msa1315110	0.821726	3.463929e-53	-8.615850e-47
Msa0479090	Msa1315110	0.809740	1.629417e-50	-8.615850e-47
Msa0482190	Msa1315110	-0.803129	4.038749e-49	-8.615850e-47
Msa0982270	Msa1315110	0.841963	3.453458e-58	-8.615850e-47
Msa1006850	Msa1315110	0.804897	1.732185e-49	-8.615850e-47
Msa1012830	Msa1315110	0.821874	3.200608e-53	-8.615850e-47
Msa1012920	Msa1315110	0.814242	1.702561e-51	-8.615850e-47
Msa1021340	Msa1315110	0.821286	4.376942e-53	-8.615850e-47
Msa1037020	Msa1315110	0.811323	7.413728e-51	-8.615850e-47
Msa1054410	Msa1315110	0.849688	2.754732e-60	-8.615850e-47
Msa1077670	Msa1315110	0.840033	1.109273e-57	-8.615850e-47
Msa1079730	Msa1315110	-0.850713	1.421240e-60	-8.615850e-47
Msa1094430	Msa1315110	0.819866	9.273827e-53	-8.615850e-47
Msa0265380	Msa1315110	0.826463	2.676007e-54	-8.615850e-47
Msa0268090	Msa1315110	0.854759	9.938937e-62	-8.615850e-47
Msa0303360	Msa1315110	0.836272	1.031148e-56	-8.615850e-47
Msa0308770	Msa1315110	0.853822	1.853628e-61	-8.615850e-47
Msa0347610	Msa1315110	0.853934	1.720129e-61	-8.615850e-47
Msa1124540	Msa1315110	-0.805006	1.644070e-49	-8.615850e-47
Msa1132890	Msa1315110	0.810911	9.109665e-51	-8.615850e-47
Msa1148750	Msa1315110	0.806725	7.154593e-50	-8.615850e-47
Msa1162980	Msa1315110	0.835264	1.856217e-56	-8.615850e-47
Msa1164530	Msa1315110	0.814425	1.550973e-51	-8.615850e-47
Msa1168040	Msa1315110	-0.853837	1.834385e-61	-8.615850e-47
Msa1174870	Msa1315110	0.993560	1.373762e-200	-8.615850e-47
Msa1176070	Msa1315110	0.802512	5.415561e-49	-8.615850e-47
Msa1207470	Msa1315110	-0.801765	7.717006e-49	-8.615850e-47
Msa1219810	Msa1315110	0.856453	3.183856e-62	-8.615850e-47
Msa0489610	Msa1315110	0.834920	2.266973e-56	-8.615850e-47
Msa0500720	Msa1315110	0.805137	1.543539e-49	-8.615850e-47
Msa0504410	Msa1315110	0.860214	2.415024e-63	-8.615850e-47
Msa0504450	Msa1315110	0.824301	8.690814e-54	-8.615850e-47
Msa0516160	Msa1315110	0.804404	2.195839e-49	-8.615850e-47
Msa0519220	Msa1315110	0.823069	1.689433e-53	-8.615850e-47
Msa0532540	Msa1315110	0.831167	1.946125e-55	-8.615850e-47
Msa0536650	Msa1315110	0.813751	2.184092e-51	-8.615850e-47
Msa0553220	Msa1315110	-0.814155	1.779478e-51	-8.615850e-47
Msa0561130	Msa1315110	-0.828641	8.029741e-55	-8.615850e-47
Msa0580420	Msa1315110	0.827408	1.590582e-54	-8.615850e-47
Msa0589390	Msa1315110	0.825348	4.924202e-54	-8.615850e-47
Msa0592720	Msa1315110	0.814309	1.644831e-51	-8.615850e-47
Msa0859380	Msa1315110	0.812027	5.212878e-51	-8.615850e-47
Msa0864470	Msa1315110	-0.802303	5.981908e-49	-8.615850e-47
Msa0870150	Msa1315110	0.854038	1.605513e-61	-8.615850e-47
Msa0901630	Msa1315110	0.822574	2.202498e-53	-8.615850e-47
Msa0903400	Msa1315110	0.815429	9.285967e-52	-8.615850e-47
Msa0925660	Msa1315110	0.811151	8.081042e-51	-8.615850e-47
Msa0935850	Msa1315110	0.862627	4.431558e-64	-8.615850e-47
Msa0941540	Msa1315110	0.824311	8.645852e-54	-8.615850e-47
Msa0956410	Msa1315110	0.813385	2.629674e-51	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1315110	MtrunA17_Chr8g0344721	83.628	226	29	2	1	218	1	226	6.76e-134	376
Msa1315110	MtrunA17_Chr7g0271891	41.176	153	80	5	11	162	17	160	1.12e-30	111
Msa1315110	MtrunA17_Chr4g0044331	44.094	127	64	4	16	138	18	141	8.11e-28	103
Msa1315110	MtrunA17_Chr2g0282761	45.122	82	38	2	10	91	4	78	1.94e-15	74.3
Msa1315110	MtrunA17_Chr7g0263971	49.333	75	33	1	6	80	365	434	2.41e-15	74.7
Msa1315110	MtrunA17_Chr2g0324921	49.275	69	30	1	10	78	4	67	4.60e-15	73.2
Msa1315110	MtrunA17_Chr4g0021141	45.455	77	36	1	3	79	15	85	4.66e-15	69.7
Msa1315110	MtrunA17_Chr4g0061421	53.731	67	29	1	12	78	10	74	1.01e-14	72.8
Msa1315110	MtrunA17_Chr4g0006391	46.377	69	32	1	10	78	4	67	4.45e-14	70.5
Msa1315110	MtrunA17_Chr2g0282741	43.478	69	34	1	10	78	4	67	5.63e-14	70.1
Msa1315110	MtrunA17_Chr4g0040501	43.478	69	34	1	10	78	4	67	9.09e-14	69.7
Msa1315110	MtrunA17_Chr2g0295831	45.000	80	37	2	9	88	109	181	2.22e-13	67.4
Msa1315110	MtrunA17_Chr7g0258061	37.273	110	59	3	4	109	16	119	2.48e-13	67.8
Msa1315110	MtrunA17_Chr5g0427221	38.298	94	43	3	7	96	45	127	3.03e-13	65.1
Msa1315110	MtrunA17_Chr3g0138471	43.478	69	34	1	10	78	4	67	3.93e-13	67.8
Msa1315110	MtrunA17_Chr7g0269841	50.000	68	28	2	11	78	27	88	5.08e-12	64.7
Msa1315110	MtrunA17_Chr1g0209551	41.026	78	40	1	9	86	15	86	5.60e-12	64.7
Msa1315110	MtrunA17_Chr8g0378161	44.118	68	34	1	11	78	61	124	7.83e-12	62.4
Msa1315110	MtrunA17_Chr5g0399201	42.857	70	37	1	10	79	12	78	8.75e-12	64.3
Msa1315110	MtrunA17_Chr1g0173931	42.500	80	39	2	8	86	36	109	1.08e-11	63.2
Msa1315110	MtrunA17_Chr2g0295821	41.250	80	40	2	1	80	95	167	3.90e-11	62.0
Msa1315110	MtrunA17_Chr3g0123861	44.928	69	31	1	10	78	82	143	7.08e-11	61.2
Msa1315110	MtrunA17_Chr6g0460381	48.485	66	31	2	10	74	3	66	7.57e-11	59.7
Msa1315110	MtrunA17_Chr6g0460461	47.143	70	34	2	10	78	2	69	7.97e-11	60.1
Msa1315110	MtrunA17_Chr6g0460411	47.143	70	34	2	10	78	2	69	8.26e-11	60.1
Msa1315110	MtrunA17_Chr5g0415731	38.889	72	42	1	9	78	4	75	8.44e-11	58.5
Msa1315110	MtrunA17_Chr7g0269901	40.625	96	44	4	4	88	90	183	9.46e-11	61.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1315110	AT3G14200.1	51.515	231	90	8	10	233	12	227	4.01e-62	194
Msa1315110	AT1G72416.4	63.810	105	25	2	10	109	5	101	2.28e-40	137
Msa1315110	AT1G72416.2	65.049	103	23	2	10	107	5	99	2.40e-40	137
Msa1315110	AT1G72416.1	65.049	103	23	2	10	107	5	99	3.30e-40	137
Msa1315110	AT1G72416.3	65.049	103	23	2	10	107	5	99	8.61e-40	136
Msa1315110	AT1G56300.1	50.376	133	57	4	1	129	1	128	7.92e-33	117
Msa1315110	AT1G71000.1	48.611	144	58	6	4	139	2	137	9.47e-33	117
Msa1315110	AT1G71000.2	42.593	162	79	5	4	159	2	155	5.06e-28	105
Msa1315110	AT1G56300.2	51.818	110	47	3	1	106	1	108	8.15e-28	103
Msa1315110	AT1G72416.5	73.469	49	13	0	61	109	10	58	9.88e-19	80.5
Msa1315110	AT5G01390.3	49.275	69	30	1	10	78	4	67	4.19e-15	72.4
Msa1315110	AT5G01390.2	49.275	69	30	1	10	78	4	67	7.94e-15	72.4
Msa1315110	AT1G59725.1	46.377	69	33	1	10	78	4	68	1.06e-14	72.4
Msa1315110	AT5G01390.1	49.275	69	30	1	10	78	4	67	1.28e-14	72.4
Msa1315110	AT5G01390.4	49.275	69	30	1	10	78	4	67	2.30e-14	70.5
Msa1315110	AT5G49060.2	46.914	81	35	3	7	86	96	169	2.50e-14	71.6
Msa1315110	AT5G49060.1	46.914	81	35	3	7	86	96	169	2.50e-14	71.6
Msa1315110	AT5G03160.1	46.667	75	35	1	6	80	366	435	4.40e-14	71.2
Msa1315110	AT5G25530.2	44.928	69	33	1	10	78	4	67	5.79e-14	70.5
Msa1315110	AT4G28480.2	46.377	69	32	1	10	78	4	67	7.92e-14	69.7
Msa1315110	AT3G08910.1	46.377	69	32	1	10	78	4	67	9.22e-14	69.7
Msa1315110	AT4G28480.1	46.377	69	32	1	10	78	4	67	1.36e-13	69.3
Msa1315110	AT2G35720.1	42.308	78	42	1	2	79	7	81	2.15e-13	69.3
Msa1315110	AT3G47940.1	42.857	70	36	1	10	79	4	69	2.26e-13	68.9
Msa1315110	AT1G10350.1	42.029	69	35	1	10	78	4	67	4.01e-13	68.2
Msa1315110	AT5G16650.1	39.024	82	43	2	3	83	4	79	6.34e-13	64.3
Msa1315110	AT2G20560.1	44.928	69	33	1	10	78	4	67	8.73e-13	67.0
Msa1315110	AT5G16650.2	39.024	82	43	2	3	83	4	79	2.38e-12	63.5
Msa1315110	AT3G12170.1	38.793	116	61	4	1	113	1	109	3.01e-12	65.1
Msa1315110	AT2G33735.1	50.704	71	25	2	10	78	22	84	3.05e-12	62.0
Msa1315110	AT2G33735.2	50.704	71	25	2	10	78	22	84	3.05e-12	62.0
Msa1315110	AT3G12170.2	37.931	116	62	4	1	113	37	145	7.05e-12	64.3
Msa1315110	AT5G06910.1	35.556	135	54	5	1	130	19	125	1.07e-11	63.5
Msa1315110	AT5G25530.1	40.260	77	33	2	10	78	4	75	2.11e-11	63.2
Msa1315110	AT4G39960.1	39.785	93	43	3	9	97	84	167	2.55e-11	63.2
Msa1315110	AT4G39960.2	39.785	93	43	3	9	97	84	167	2.55e-11	63.2
Msa1315110	AT1G80030.4	42.254	71	34	1	8	78	73	136	4.74e-11	62.4
Msa1315110	AT1G80030.2	42.254	71	34	1	8	78	73	136	6.24e-11	62.0
Msa1315110	AT1G80030.3	42.254	71	34	1	8	78	73	136	6.24e-11	62.0
Msa1315110	AT1G80030.1	42.254	71	34	1	8	78	73	136	6.24e-11	62.0
Msa1315110	AT3G62600.1	48.529	68	29	2	11	78	27	88	8.37e-11	61.2

Find 69 sgRNAs with CRISPR-Local

Find 266 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAGAAGTTCTGGTAATT+TGG	0.142853	8_4:-14376650	None:intergenic
AATTCTACTCAGATGAATTA+TGG	0.200136	8_4:+14376599	Msa1315110:CDS
TCTAGTTGGTTGTGAAAATT+TGG	0.205516	8_4:+14374012	Msa1315110:five_prime_UTR
TGTTATTGTTGGAAATAATA+TGG	0.247286	8_4:+14374037	Msa1315110:exon
ATTAGTCATTTGTTGCATTA+TGG	0.247313	8_4:+14378004	Msa1315110:three_prime_UTR
GAAGATGTGGAGAAGGAAAA+AGG	0.260664	8_4:+14377559	Msa1315110:CDS
CCTTCTCCACATCTTCTTGA+AGG	0.267807	8_4:-14377552	None:intergenic
GTGTGTCAACCTTAGCATTT+TGG	0.273693	8_4:+14377719	Msa1315110:three_prime_UTR
CAATGATTTCTATGCAGTTT+TGG	0.279803	8_4:+14374079	Msa1315110:CDS
CCAGATCGTTGTTCAGCTTC+AGG	0.283002	8_4:+14374423	Msa1315110:CDS
AATTACCAGAACTTCTGTTT+TGG	0.283009	8_4:+14376653	Msa1315110:CDS
GGCTCACATTTGATTTAGTT+TGG	0.297419	8_4:-14375757	None:intergenic
CAAGCGGATATCGGATTAAA+TGG	0.298600	8_4:+14376494	Msa1315110:CDS
GCAGAGGATTCTTCTAGTTT+TGG	0.305479	8_4:+14376626	Msa1315110:CDS
AGCACCTCTCTTAATGCTTC+GGG	0.329574	8_4:+14376518	Msa1315110:CDS
CTTAAACTCAAACTCCTAGA+TGG	0.329984	8_4:-14377896	None:intergenic
CTTCAACTTGGATGACAACT+TGG	0.330506	8_4:-14373763	None:intergenic
CTAATGTTTCTTTGTATTGT+CGG	0.332006	8_4:-14373941	None:intergenic
TTATATGTGATACAGGAAAA+TGG	0.333099	8_4:+14376425	Msa1315110:intron
TATATAAAGTCTAGAATTAG+AGG	0.347820	8_4:+14373630	Msa1315110:five_prime_UTR
GCAATTCAAGAAGCCTATTC+TGG	0.348966	8_4:+14374486	Msa1315110:CDS
AGAAGCTAAGAAGAAATTTC+AGG	0.357982	8_4:+14374464	Msa1315110:CDS
TCATCTACTTTCATGACTTT+CGG	0.362653	8_4:+14376551	Msa1315110:CDS
AAATGCCTTGGAAAATTTGA+TGG	0.373884	8_4:+14377843	Msa1315110:three_prime_UTR
GTTTGGTTGGTTGATCTAGT+TGG	0.390684	8_4:+14373998	Msa1315110:five_prime_UTR
AAGCACCTCTCTTAATGCTT+CGG	0.391365	8_4:+14376517	Msa1315110:CDS
GATATGTTTCAAGCGGATAT+CGG	0.410739	8_4:+14376485	Msa1315110:CDS
TCTGTTGGAGAATTCTAGTT+TGG	0.411390	8_4:+14373981	Msa1315110:five_prime_UTR
ACATTAGTTTGATATTTGAT+TGG	0.420967	8_4:+14373957	Msa1315110:five_prime_UTR
ATTCTACTCAGATGAATTAT+GGG	0.423184	8_4:+14376600	Msa1315110:CDS
TGACCCCAAAACAGAAGTTC+TGG	0.428777	8_4:-14376658	None:intergenic
AAAATTTGGATTGTTATTGT+TGG	0.435217	8_4:+14374026	Msa1315110:five_prime_UTR
CAGATCGTTGTTCAGCTTCA+GGG	0.447334	8_4:+14374424	Msa1315110:CDS
AAGCAATTGTACACCCATCT+AGG	0.450605	8_4:+14377882	Msa1315110:three_prime_UTR
TGCATGTAAATTACCAGAAT+AGG	0.452286	8_4:-14374499	None:intergenic
AAATGGAGAGGAGAGCTTTG+AGG	0.455188	8_4:+14376442	Msa1315110:CDS
TATCAATTAGCAGAAATTCC+AGG	0.462490	8_4:-14377673	None:intergenic
CCTGAAGCTGAACAACGATC+TGG	0.468892	8_4:-14374423	None:intergenic
AATTCCAGGATGGTCATTAG+AGG	0.478304	8_4:-14377659	None:intergenic
GGGAGACTTTCTGAACGAAA+TGG	0.482099	8_4:+14375721	Msa1315110:CDS
TTGGATGACAACTTGGGTTG+CGG	0.483218	8_4:-14373756	None:intergenic
TACTAAGATAAGATAAACTA+TGG	0.485649	8_4:-14377799	None:intergenic
GGAAATAATATGGCTAATGA+AGG	0.487826	8_4:+14374047	Msa1315110:exon
TTGGAGAATTCTAGTTTGGT+TGG	0.496008	8_4:+14373985	Msa1315110:five_prime_UTR
TCATGACTTTCGGTGAAAGC+GGG	0.503824	8_4:+14376561	Msa1315110:CDS
TTCCCATGACCAAAATGCTA+AGG	0.508517	8_4:-14377728	None:intergenic
TGATATTTGATTGGTTCTGT+TGG	0.511150	8_4:+14373966	Msa1315110:five_prime_UTR
AATGCTCTGATTCAGAACTA+AGG	0.513112	8_4:+14374114	Msa1315110:CDS
TTCAGGGAATGTGAAGTTTG+TGG	0.516942	8_4:+14374440	Msa1315110:CDS
ATGGGTGTACAATTGCTTGA+AGG	0.522891	8_4:-14377877	None:intergenic
TTAGGAGGTCACAAATGCCT+TGG	0.526911	8_4:+14377831	Msa1315110:three_prime_UTR
AGTCATGAAAGTAGATGAAG+TGG	0.538936	8_4:-14376546	None:intergenic
TGCTTATAAGAAACTTGCAC+TGG	0.543386	8_4:+14374139	Msa1315110:CDS
TTCAACTTGGATGACAACTT+GGG	0.545053	8_4:-14373762	None:intergenic
ATTAATATACAACTTCAACT+TGG	0.554791	8_4:-14373775	None:intergenic
TTAAACTCAAACTCCTAGAT+GGG	0.559329	8_4:-14377895	None:intergenic
GTTTCCTCTAATGACCATCC+TGG	0.561659	8_4:+14377655	Msa1315110:CDS
ATTTGATGATATGTTTCAAG+CGG	0.571158	8_4:+14376478	Msa1315110:CDS
TTTAGACTAGATGGAATGAA+GGG	0.573998	8_4:-14373692	None:intergenic
TTCATGACTTTCGGTGAAAG+CGG	0.579461	8_4:+14376560	Msa1315110:CDS
CCTTCAAGAAGATGTGGAGA+AGG	0.586502	8_4:+14377552	Msa1315110:CDS
GAAGCACCTTCAAGAAGATG+TGG	0.587778	8_4:+14377546	Msa1315110:CDS
GATGAATTATGGGAAGGCAG+AGG	0.603296	8_4:+14376610	Msa1315110:CDS
TACTCAGATGAATTATGGGA+AGG	0.609702	8_4:+14376604	Msa1315110:CDS
TGTGATACAGGAAAATGGAG+AGG	0.651222	8_4:+14376430	Msa1315110:intron
AAGAGATTAATGTACGACGT+TGG	0.670458	8_4:+14374666	Msa1315110:CDS
AATTAGCAGAAATTCCAGGA+TGG	0.683865	8_4:-14377669	None:intergenic
GCAACCCGAAGCATTAAGAG+AGG	0.689028	8_4:-14376522	None:intergenic
AGGTCTCTAGAGTATTCATG+AGG	0.690314	8_4:+14373650	Msa1315110:five_prime_UTR

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTGATTATATGAAAAAAAAT+CGG	-	chr8_4:14375311-14375330	None:intergenic	10.0%
!!	TATTGCAAAAATATTAAATA+GGG	-	chr8_4:14375781-14375800	None:intergenic	10.0%
!!	ATATTGCAAAAATATTAAAT+AGG	-	chr8_4:14375782-14375801	None:intergenic	10.0%
!!!	TTTATATATTTTTTGTTTAG+TGG	+	chr8_4:14375878-14375897	Msa1315110:intron	10.0%
!!	TATTTAAAAAATAATTCAAG+TGG	+	chr8_4:14376118-14376137	Msa1315110:intron	10.0%
!!!	TGAATAAACTTTAGAAAAAA+CGG	-	chr8_4:14374237-14374256	None:intergenic	15.0%
!!	AGGAAAATAATTTGATTAAA+TGG	+	chr8_4:14374387-14374406	Msa1315110:intron	15.0%
!!	ACAAATAATAATTTGAATCA+TGG	-	chr8_4:14374582-14374601	None:intergenic	15.0%
!!!	TTCTCAATCTATTTTTTAAA+CGG	-	chr8_4:14375014-14375033	None:intergenic	15.0%
!!	TGATTATATGAAAAAAAATC+GGG	-	chr8_4:14375310-14375329	None:intergenic	15.0%
!!!	AACAAGATATTATTTTATTG+TGG	+	chr8_4:14376159-14376178	Msa1315110:intron	15.0%
!!!	CTTTAAATTATACAAGATTT+TGG	+	chr8_4:14376880-14376899	Msa1315110:intron	15.0%
!!!	TATATAAAGTCTAGAATTAG+AGG	+	chr8_4:14373630-14373649	Msa1315110:five_prime_UTR	20.0%
!!	ATTAATATACAACTTCAACT+TGG	-	chr8_4:14373778-14373797	None:intergenic	20.0%
!!!	GTTTCTTACTTTTTATGTTA+AGG	+	chr8_4:14373873-14373892	Msa1315110:five_prime_UTR	20.0%
!!!	ACGAATTTTGTGTTTTTATT+TGG	+	chr8_4:14373900-14373919	Msa1315110:five_prime_UTR	20.0%
!!!	TTTTGTGTTTTTATTTGGTT+TGG	+	chr8_4:14373905-14373924	Msa1315110:five_prime_UTR	20.0%
!!!	ACATTAGTTTGATATTTGAT+TGG	+	chr8_4:14373957-14373976	Msa1315110:five_prime_UTR	20.0%
!!!	AAAATTTGGATTGTTATTGT+TGG	+	chr8_4:14374026-14374045	Msa1315110:five_prime_UTR	20.0%
!!	TGTTATTGTTGGAAATAATA+TGG	+	chr8_4:14374037-14374056	Msa1315110:exon	20.0%
!!!	TTTTCTAAAGTTTATTCACA+TGG	+	chr8_4:14374239-14374258	Msa1315110:intron	20.0%
!!!	TTTCTAAAGTTTATTCACAT+GGG	+	chr8_4:14374240-14374259	Msa1315110:intron	20.0%
!!	TATCTTTCTTTCACAAATTT+AGG	-	chr8_4:14374317-14374336	None:intergenic	20.0%
!!!	TTTGTGAAAGAAAGATATTT+TGG	+	chr8_4:14374320-14374339	Msa1315110:intron	20.0%
!!!	AATGTAAATGGTGTTTTTTT+TGG	+	chr8_4:14374621-14374640	Msa1315110:intron	20.0%
!!!	ATGTAAATGGTGTTTTTTTT+GGG	+	chr8_4:14374622-14374641	Msa1315110:intron	20.0%
!!	AAAGTGAAAAGGTAAAATTA+AGG	-	chr8_4:14374841-14374860	None:intergenic	20.0%
!!	ATTCAGTTTCAATATTCATT+AGG	-	chr8_4:14374893-14374912	None:intergenic	20.0%
!!	ATGAATATTGAAACTGAATT+AGG	+	chr8_4:14374894-14374913	Msa1315110:intron	20.0%
!!	TGAATATTGAAACTGAATTA+GGG	+	chr8_4:14374895-14374914	Msa1315110:intron	20.0%
!!!	TGGAAATGAGTTAATTTTTT+AGG	+	chr8_4:14375114-14375133	Msa1315110:intron	20.0%
!!!	GTTTAAAATAGTGACTTAAT+TGG	-	chr8_4:14375188-14375207	None:intergenic	20.0%
!!	TTATAGCACATCAAAATTAT+TGG	-	chr8_4:14375669-14375688	None:intergenic	20.0%
!!!	AAAATGAATTTTGTTTTGCA+GGG	+	chr8_4:14375695-14375714	Msa1315110:intron	20.0%
!!	AACAAGACATAAACATTATT+AGG	-	chr8_4:14375980-14375999	None:intergenic	20.0%
!!!	AATAATGTTTATGTCTTGTT+TGG	+	chr8_4:14375980-14375999	Msa1315110:intron	20.0%
!!	CAAGATTTGAAAATAATTGT+TGG	+	chr8_4:14376193-14376212	Msa1315110:intron	20.0%
!!	ATTTGAAAATAATTGTTGGA+AGG	+	chr8_4:14376197-14376216	Msa1315110:intron	20.0%
!!!	AATCAATTTATGAAAAGTAC+AGG	-	chr8_4:14376302-14376321	None:intergenic	20.0%
!!!	AGTTAAAAGGATTGAAATTT+TGG	-	chr8_4:14376364-14376383	None:intergenic	20.0%
!!	AATGAATTGAAAGAGTTAAA+AGG	-	chr8_4:14376377-14376396	None:intergenic	20.0%
!!	GATTAATTTATATGTGATAC+AGG	+	chr8_4:14376418-14376437	Msa1315110:intron	20.0%
!!	TTTAACATGTAATTGAGTTA+TGG	+	chr8_4:14376851-14376870	Msa1315110:intron	20.0%
!!	TTAACATGTAATTGAGTTAT+GGG	+	chr8_4:14376852-14376871	Msa1315110:intron	20.0%
!!!	ATGATAGAAGATTTAGAATT+TGG	+	chr8_4:14376913-14376932	Msa1315110:intron	20.0%
!!	ACTCTATTAATAACTCAAAA+TGG	-	chr8_4:14377355-14377374	None:intergenic	20.0%
!!	TTTGTATCAAATTCTCAAAT+TGG	-	chr8_4:14377384-14377403	None:intergenic	20.0%
!!!	TGCAATTTTTTTAAGAAGAT+TGG	+	chr8_4:14377462-14377481	Msa1315110:intron	20.0%
!!!	TCCAACATTTTTTTTATTCT+TGG	+	chr8_4:14377510-14377529	Msa1315110:intron	20.0%
!!	ACCAAGAATAAAAAAAATGT+TGG	-	chr8_4:14377514-14377533	None:intergenic	20.0%
!!	ATGTACAACTAAAAAGATTA+GGG	+	chr8_4:14377770-14377789	Msa1315110:three_prime_UTR	20.0%
!!	TACTAAGATAAGATAAACTA+TGG	-	chr8_4:14377802-14377821	None:intergenic	20.0%
!!	ATCTTAGTACTAAAAAGATT+AGG	+	chr8_4:14377813-14377832	Msa1315110:three_prime_UTR	20.0%
!!!	AATGTCAATTTTGATTTGAA+AGG	+	chr8_4:14378037-14378056	Msa1315110:three_prime_UTR	20.0%
!	CTAATGTTTCTTTGTATTGT+CGG	-	chr8_4:14373944-14373963	None:intergenic	25.0%
!!	AATGATTTCTATGCAGTTTT+GGG	+	chr8_4:14374080-14374099	Msa1315110:CDS	25.0%
!!	ATAAACTTTAGAAAAAACGG+TGG	-	chr8_4:14374234-14374253	None:intergenic	25.0%
!!	ATTGTTGTTATGATGAAAGT+TGG	+	chr8_4:14374352-14374371	Msa1315110:intron	25.0%
!	AAAGTTGGAATTGTTTAGAA+AGG	+	chr8_4:14374367-14374386	Msa1315110:intron	25.0%
!	TTTGATTAAATGGACAGAAA+TGG	+	chr8_4:14374397-14374416	Msa1315110:intron	25.0%
!!	TATTGGGTCTAAAATGTAAA+TGG	+	chr8_4:14374609-14374628	Msa1315110:intron	25.0%
!	CATAACACAAAAATAGAAGT+AGG	-	chr8_4:14374776-14374795	None:intergenic	25.0%
!!	TTGTTTCTTCATTTACTTGA+AGG	+	chr8_4:14374798-14374817	Msa1315110:intron	25.0%
!	TGTTTCTTCATTTACTTGAA+GGG	+	chr8_4:14374799-14374818	Msa1315110:intron	25.0%
!	TTGTTAATGTGAAAGTGAAA+AGG	-	chr8_4:14374852-14374871	None:intergenic	25.0%
!!	CACTTTCACATTAACAATTT+TGG	+	chr8_4:14374855-14374874	Msa1315110:intron	25.0%
!!	TATAAGGATGATTTGATAGT+TGG	+	chr8_4:14374919-14374938	Msa1315110:intron	25.0%
!	AACAATGATATGGTCAATTT+CGG	+	chr8_4:14375069-14375088	Msa1315110:intron	25.0%
!	CAACGAAAACAAGAAAAAAA+TGG	+	chr8_4:14375094-14375113	Msa1315110:intron	25.0%
!!!	AGTCACTATTTTAAACCTAA+AGG	+	chr8_4:14375192-14375211	Msa1315110:intron	25.0%
!	AAGAGGAGAAGAAAAATAAT+TGG	-	chr8_4:14375414-14375433	None:intergenic	25.0%
!!!	ACATTTTTTTTTCCTCCTTT+GGG	-	chr8_4:14375504-14375523	None:intergenic	25.0%
!!!	AACATTTTTTTTTCCTCCTT+TGG	-	chr8_4:14375505-14375524	None:intergenic	25.0%
!	GATTCAATTCCATGAATTAT+TGG	-	chr8_4:14375625-14375644	None:intergenic	25.0%
!	ATTCATGGAATTGAATCAAA+TGG	+	chr8_4:14375628-14375647	Msa1315110:intron	25.0%
!!!	GAAAATGAATTTTGTTTTGC+AGG	+	chr8_4:14375694-14375713	Msa1315110:intron	25.0%
!!!	TTTTGTTTGCTTGTAATTAG+TGG	+	chr8_4:14375809-14375828	Msa1315110:intron	25.0%
!	TTATATGTGATACAGGAAAA+TGG	+	chr8_4:14376425-14376444	Msa1315110:intron	25.0%
!!	ATTTGATGATATGTTTCAAG+CGG	+	chr8_4:14376478-14376497	Msa1315110:CDS	25.0%
!	AATTCTACTCAGATGAATTA+TGG	+	chr8_4:14376599-14376618	Msa1315110:CDS	25.0%
!	ATTCTACTCAGATGAATTAT+GGG	+	chr8_4:14376600-14376619	Msa1315110:CDS	25.0%
!	TTGAATGATTGTTTATTGTG+AGG	+	chr8_4:14376776-14376795	Msa1315110:intron	25.0%
!	AGTAGTAACAACAAAAAGAT+AGG	+	chr8_4:14376828-14376847	Msa1315110:intron	25.0%
!	TAACATGTAATTGAGTTATG+GGG	+	chr8_4:14376853-14376872	Msa1315110:intron	25.0%
!	TAATCTATCTTCAGAGTAAT+TGG	-	chr8_4:14377081-14377100	None:intergenic	25.0%
!	TTAAGTGGTTAAGGAAAAAA+TGG	+	chr8_4:14377098-14377117	Msa1315110:intron	25.0%
!	CACTAATTAAAAGACTCATT+GGG	-	chr8_4:14377146-14377165	None:intergenic	25.0%
!!!	TTTTGTTGTTCATGTTATCA+AGG	+	chr8_4:14377173-14377192	Msa1315110:intron	25.0%
!	ATTATCATTACCAATGAATG+AGG	+	chr8_4:14377208-14377227	Msa1315110:intron	25.0%
!	TTACAAAACTAATAGAGTCA+GGG	+	chr8_4:14377296-14377315	Msa1315110:intron	25.0%
!	AGAAAACAAAAAATCTCATG+TGG	+	chr8_4:14377625-14377644	Msa1315110:CDS	25.0%
!	CATGTACAACTAAAAAGATT+AGG	+	chr8_4:14377769-14377788	Msa1315110:three_prime_UTR	25.0%
!	TGTACAACTAAAAAGATTAG+GGG	+	chr8_4:14377771-14377790	Msa1315110:three_prime_UTR	25.0%
!	TTAGTACTAAAAAGATTAGG+AGG	+	chr8_4:14377816-14377835	Msa1315110:three_prime_UTR	25.0%
!!	ATTAGTCATTTGTTGCATTA+TGG	+	chr8_4:14378004-14378023	Msa1315110:three_prime_UTR	25.0%
	TTTAGACTAGATGGAATGAA+GGG	-	chr8_4:14373695-14373714	None:intergenic	30.0%
!	TTTTAGACTAGATGGAATGA+AGG	-	chr8_4:14373696-14373715	None:intergenic	30.0%
!!!	GCATTTTGTTTTAGACTAGA+TGG	-	chr8_4:14373704-14373723	None:intergenic	30.0%
!!	TGATATTTGATTGGTTCTGT+TGG	+	chr8_4:14373966-14373985	Msa1315110:five_prime_UTR	30.0%
	TCTAGTTGGTTGTGAAAATT+TGG	+	chr8_4:14374012-14374031	Msa1315110:five_prime_UTR	30.0%
	GGAAATAATATGGCTAATGA+AGG	+	chr8_4:14374047-14374066	Msa1315110:exon	30.0%
!	CAATGATTTCTATGCAGTTT+TGG	+	chr8_4:14374079-14374098	Msa1315110:CDS	30.0%
	AGAAGCTAAGAAGAAATTTC+AGG	+	chr8_4:14374464-14374483	Msa1315110:CDS	30.0%
	TGCATGTAAATTACCAGAAT+AGG	-	chr8_4:14374502-14374521	None:intergenic	30.0%
	TAATTTGAATCATGGCTCAT+GGG	-	chr8_4:14374574-14374593	None:intergenic	30.0%
	ATAATTTGAATCATGGCTCA+TGG	-	chr8_4:14374575-14374594	None:intergenic	30.0%
	GTTTCAATATTCATTAGGCA+TGG	-	chr8_4:14374888-14374907	None:intergenic	30.0%
	GAAACTGAATTAGGGATATA+AGG	+	chr8_4:14374903-14374922	Msa1315110:intron	30.0%
	GGAGAAGAAAAATAATTGGA+CGG	-	chr8_4:14375410-14375429	None:intergenic	30.0%
	AAAAGGAGATATGTTCTTCA+AGG	+	chr8_4:14375442-14375461	Msa1315110:intron	30.0%
	ATATGTTCTTCAAGGTTAGT+AGG	+	chr8_4:14375450-14375469	Msa1315110:intron	30.0%
	TTTGGGAAAAGACATTTGTT+GGG	-	chr8_4:14375487-14375506	None:intergenic	30.0%
	CAATAAGAGCCAATAATTCA+TGG	+	chr8_4:14375613-14375632	Msa1315110:intron	30.0%
!!!	AATTTTGTTTTGCAGGGTAT+GGG	+	chr8_4:14375701-14375720	Msa1315110:intron	30.0%
	TAATTGTTGGAAGGATAAAG+TGG	+	chr8_4:14376206-14376225	Msa1315110:intron	30.0%
	TCATCTACTTTCATGACTTT+CGG	+	chr8_4:14376551-14376570	Msa1315110:CDS	30.0%
!	AAACAGAAGTTCTGGTAATT+TGG	-	chr8_4:14376653-14376672	None:intergenic	30.0%
!	AATTACCAGAACTTCTGTTT+TGG	+	chr8_4:14376653-14376672	Msa1315110:CDS	30.0%
!	ATTACCAGAACTTCTGTTTT+GGG	+	chr8_4:14376654-14376673	Msa1315110:CDS	30.0%
	ATGAATGTTGCATCAAATGA+AGG	-	chr8_4:14376714-14376733	None:intergenic	30.0%
	TTCATTTGATGCAACATTCA+TGG	+	chr8_4:14376713-14376732	Msa1315110:intron	30.0%
	ATAACAATTCATGCTTCAGA+TGG	-	chr8_4:14377040-14377059	None:intergenic	30.0%
!	TACTCTGAAGATAGATTAAG+TGG	+	chr8_4:14377083-14377102	Msa1315110:intron	30.0%
	GAAGATAGATTAAGTGGTTA+AGG	+	chr8_4:14377089-14377108	Msa1315110:intron	30.0%
	CCACTAATTAAAAGACTCAT+TGG	-	chr8_4:14377147-14377166	None:intergenic	30.0%
!!	CCAATGAGTCTTTTAATTAG+TGG	+	chr8_4:14377144-14377163	Msa1315110:intron	30.0%
!	TGTTTTGTATCCTCATTCAT+TGG	-	chr8_4:14377221-14377240	None:intergenic	30.0%
	TGAATGAGGATACAAAACAA+TGG	+	chr8_4:14377222-14377241	Msa1315110:intron	30.0%
!!!	CTGACTCTATTAGTTTTGTA+AGG	-	chr8_4:14377297-14377316	None:intergenic	30.0%
	CTTACAAAACTAATAGAGTC+AGG	+	chr8_4:14377295-14377314	Msa1315110:intron	30.0%
	AAAGACAGAGAGTAATTTGA+TGG	+	chr8_4:14377401-14377420	Msa1315110:intron	30.0%
	CACTCCATATATTATATAGC+TGG	+	chr8_4:14377426-14377445	Msa1315110:intron	30.0%
	GAGACCAGCTATATAATATA+TGG	-	chr8_4:14377433-14377452	None:intergenic	30.0%
	CAGTATCAAGTTAAATGTAG+TGG	-	chr8_4:14377489-14377508	None:intergenic	30.0%
	GGTAGCAAAAAACAAAGAAA+TGG	+	chr8_4:14377601-14377620	Msa1315110:CDS	30.0%
	TATCAATTAGCAGAAATTCC+AGG	-	chr8_4:14377676-14377695	None:intergenic	30.0%
	GTACAACTAAAAAGATTAGG+GGG	+	chr8_4:14377772-14377791	Msa1315110:three_prime_UTR	30.0%
	AAATGCCTTGGAAAATTTGA+TGG	+	chr8_4:14377843-14377862	Msa1315110:three_prime_UTR	30.0%
	TTAAACTCAAACTCCTAGAT+GGG	-	chr8_4:14377898-14377917	None:intergenic	30.0%
	TTCAACTTGGATGACAACTT+GGG	-	chr8_4:14373765-14373784	None:intergenic	35.0%
!	TCTGTTGGAGAATTCTAGTT+TGG	+	chr8_4:14373981-14374000	Msa1315110:five_prime_UTR	35.0%
!!	TTGGAGAATTCTAGTTTGGT+TGG	+	chr8_4:14373985-14374004	Msa1315110:five_prime_UTR	35.0%
!!!	TGCAGTTTTGGGATTGAATA+AGG	+	chr8_4:14374091-14374110	Msa1315110:CDS	35.0%
	AATGCTCTGATTCAGAACTA+AGG	+	chr8_4:14374114-14374133	Msa1315110:CDS	35.0%
	TGCTTATAAGAAACTTGCAC+TGG	+	chr8_4:14374139-14374158	Msa1315110:CDS	35.0%
	GGTTAGAGTAGAAACAAAGA+CGG	-	chr8_4:14374206-14374225	None:intergenic	35.0%
!	ACTTTAGAAAAAACGGTGGA+GGG	-	chr8_4:14374230-14374249	None:intergenic	35.0%
!	AACTTTAGAAAAAACGGTGG+AGG	-	chr8_4:14374231-14374250	None:intergenic	35.0%
	AAGAGATTAATGTACGACGT+TGG	+	chr8_4:14374666-14374685	Msa1315110:CDS	35.0%
	ATTTCACACCATTCATGAGT+TGG	+	chr8_4:14374728-14374747	Msa1315110:intron	35.0%
	TTTCACACCATTCATGAGTT+GGG	+	chr8_4:14374729-14374748	Msa1315110:intron	35.0%
!	AAGGATGATTTGATAGTTGG+TGG	+	chr8_4:14374922-14374941	Msa1315110:intron	35.0%
!	AGGATGATTTGATAGTTGGT+GGG	+	chr8_4:14374923-14374942	Msa1315110:intron	35.0%
	TTAAAGATCAGAGTGATGAG+CGG	+	chr8_4:14374950-14374969	Msa1315110:intron	35.0%
	TGAGAATGAATTTCCTCTCT+TGG	+	chr8_4:14375028-14375047	Msa1315110:intron	35.0%
	TTGACCATATCATTGTTTCG+TGG	-	chr8_4:14375066-14375085	None:intergenic	35.0%
	TCACTGTCTTCTTTACCTTT+AGG	-	chr8_4:14375210-14375229	None:intergenic	35.0%
!	TTGGGAAAAGACATTTGTTG+GGG	-	chr8_4:14375486-14375505	None:intergenic	35.0%
!	CTTTGGGAAAAGACATTTGT+TGG	-	chr8_4:14375488-14375507	None:intergenic	35.0%
!	CAACAAATGTCTTTTCCCAA+AGG	+	chr8_4:14375486-14375505	Msa1315110:intron	35.0%
	TATGTGTCACATAACACATG+TGG	+	chr8_4:14375565-14375584	Msa1315110:intron	35.0%
	CATGGAATTGAATCAAATGG+AGG	+	chr8_4:14375631-14375650	Msa1315110:intron	35.0%
!!!	GAATTTTGTTTTGCAGGGTA+TGG	+	chr8_4:14375700-14375719	Msa1315110:intron	35.0%
!	GGCTCACATTTGATTTAGTT+TGG	-	chr8_4:14375760-14375779	None:intergenic	35.0%
	CTTGTAATTAGTGGAACGTT+AGG	+	chr8_4:14375818-14375837	Msa1315110:intron	35.0%
	AATTAGTGGAACGTTAGGTT+AGG	+	chr8_4:14375823-14375842	Msa1315110:intron	35.0%
	AGGCACAACTACATAGTATT+AGG	-	chr8_4:14375936-14375955	None:intergenic	35.0%
!	AGTTGTGCCTTTTTACCTTT+TGG	+	chr8_4:14375946-14375965	Msa1315110:intron	35.0%
!	GTTGTGCCTTTTTACCTTTT+GGG	+	chr8_4:14375947-14375966	Msa1315110:intron	35.0%
!!!	CCTTTTTACCTTTTGGGATT+GGG	+	chr8_4:14375953-14375972	Msa1315110:intron	35.0%
	ACACCAACTATCATAGCAAT+TGG	-	chr8_4:14376007-14376026	None:intergenic	35.0%
!	AATTGCTATGATAGTTGGTG+TGG	+	chr8_4:14376006-14376025	Msa1315110:intron	35.0%
	GATATGTTTCAAGCGGATAT+CGG	+	chr8_4:14376485-14376504	Msa1315110:CDS	35.0%
	AGTCATGAAAGTAGATGAAG+TGG	-	chr8_4:14376549-14376568	None:intergenic	35.0%
	TACTCAGATGAATTATGGGA+AGG	+	chr8_4:14376604-14376623	Msa1315110:CDS	35.0%
!	TTACCAGAACTTCTGTTTTG+GGG	+	chr8_4:14376655-14376674	Msa1315110:CDS	35.0%
	GGCAAACATATAGCATAGTA+AGG	+	chr8_4:14376734-14376753	Msa1315110:intron	35.0%
	TGTTACTACTTACTACCCAT+AGG	-	chr8_4:14376818-14376837	None:intergenic	35.0%
	TATCTTCAGAGTAATTGGCA+AGG	-	chr8_4:14377076-14377095	None:intergenic	35.0%
	ACAAAACAATGGAGTATGTG+TGG	+	chr8_4:14377233-14377252	Msa1315110:intron	35.0%
	ATGTGTGGAGTATATAGAAG+TGG	+	chr8_4:14377248-14377267	Msa1315110:intron	35.0%
!!	AGTTTTGTAAGGAGATCACT+AGG	-	chr8_4:14377286-14377305	None:intergenic	35.0%
	TTGTCAAACGTTTCTCAGAT+TGG	-	chr8_4:14377323-14377342	None:intergenic	35.0%
	AAGATGTGGAGAAGGAAAAA+GGG	+	chr8_4:14377560-14377579	Msa1315110:CDS	35.0%
	AATTAGCAGAAATTCCAGGA+TGG	-	chr8_4:14377672-14377691	None:intergenic	35.0%
!!	AACCTTAGCATTTTGGTCAT+GGG	+	chr8_4:14377726-14377745	Msa1315110:three_prime_UTR	35.0%
!	CACTACCATCAAATTTTCCA+AGG	-	chr8_4:14377851-14377870	None:intergenic	35.0%
	CTTAAACTCAAACTCCTAGA+TGG	-	chr8_4:14377899-14377918	None:intergenic	35.0%
!!	AGGTCTCTAGAGTATTCATG+AGG	+	chr8_4:14373650-14373669	Msa1315110:five_prime_UTR	40.0%
	CTTCAACTTGGATGACAACT+TGG	-	chr8_4:14373766-14373785	None:intergenic	40.0%
!	GTTTGGTTGGTTGATCTAGT+TGG	+	chr8_4:14373998-14374017	Msa1315110:five_prime_UTR	40.0%
	TTCAGGGAATGTGAAGTTTG+TGG	+	chr8_4:14374440-14374459	Msa1315110:CDS	40.0%
	GCAATTCAAGAAGCCTATTC+TGG	+	chr8_4:14374486-14374505	Msa1315110:CDS	40.0%
	AGGCAAGTGAAAGAGAAAGA+GGG	-	chr8_4:14374756-14374775	None:intergenic	40.0%
	TAGGCAAGTGAAAGAGAAAG+AGG	-	chr8_4:14374757-14374776	None:intergenic	40.0%
!	GGATGATTTGATAGTTGGTG+GGG	+	chr8_4:14374924-14374943	Msa1315110:intron	40.0%
	TAAAGATCAGAGTGATGAGC+GGG	+	chr8_4:14374951-14374970	Msa1315110:intron	40.0%
	GAAGCAAATATGACCAAGAG+AGG	-	chr8_4:14375044-14375063	None:intergenic	40.0%
	GCTTCCACGAAACAATGATA+TGG	+	chr8_4:14375059-14375078	Msa1315110:intron	40.0%
	TCTCCTCTTTCACGTGAAAA+AGG	+	chr8_4:14375425-14375444	Msa1315110:intron	40.0%
!!	TCTCCTTTTTCACGTGAAAG+AGG	-	chr8_4:14375431-14375450	None:intergenic	40.0%
!	CAAATGTCTTTTCCCAAAGG+AGG	+	chr8_4:14375489-14375508	Msa1315110:intron	40.0%
!!!	GCCTTTTTACCTTTTGGGAT+TGG	+	chr8_4:14375952-14375971	Msa1315110:intron	40.0%
	CCCAATCCCAAAAGGTAAAA+AGG	-	chr8_4:14375956-14375975	None:intergenic	40.0%
	TATTAGGACCCAATCCCAAA+AGG	-	chr8_4:14375964-14375983	None:intergenic	40.0%
	GGTCCAATTGCTATGATAGT+TGG	+	chr8_4:14376001-14376020	Msa1315110:intron	40.0%
	GGAAGGATAAAGTGGTTTAC+TGG	+	chr8_4:14376214-14376233	Msa1315110:intron	40.0%
	GATAAAGTGGTTTACTGGAC+CGG	+	chr8_4:14376219-14376238	Msa1315110:intron	40.0%
	ATAAAGTGGTTTACTGGACC+GGG	+	chr8_4:14376220-14376239	Msa1315110:intron	40.0%
	TGTGATACAGGAAAATGGAG+AGG	+	chr8_4:14376430-14376449	Msa1315110:intron	40.0%
	CAAGCGGATATCGGATTAAA+TGG	+	chr8_4:14376494-14376513	Msa1315110:CDS	40.0%
	AAGCACCTCTCTTAATGCTT+CGG	+	chr8_4:14376517-14376536	Msa1315110:CDS	40.0%
	TTCATGACTTTCGGTGAAAG+CGG	+	chr8_4:14376560-14376579	Msa1315110:CDS	40.0%
!!!	GCAGAGGATTCTTCTAGTTT+TGG	+	chr8_4:14376626-14376645	Msa1315110:CDS	40.0%
	TGTGAGGTCCTTTACTTGTT+TGG	+	chr8_4:14376792-14376811	Msa1315110:intron	40.0%
	TCCTTTACTTGTTTGGCCTA+TGG	+	chr8_4:14376799-14376818	Msa1315110:intron	40.0%
	CCTTTACTTGTTTGGCCTAT+GGG	+	chr8_4:14376800-14376819	Msa1315110:intron	40.0%
	GATGCAACTATTGTAGTCGT+TGG	+	chr8_4:14376957-14376976	Msa1315110:intron	40.0%
!	AATGCACATTTGTTGGATCC+TGG	-	chr8_4:14376987-14377006	None:intergenic	40.0%
	TGCAACGAATGCACATTTGT+TGG	-	chr8_4:14376994-14377013	None:intergenic	40.0%
	GAAGATGTGGAGAAGGAAAA+AGG	+	chr8_4:14377559-14377578	Msa1315110:CDS	40.0%
	AGATGTGGAGAAGGAAAAAG+GGG	+	chr8_4:14377561-14377580	Msa1315110:CDS	40.0%
	GAAAAAGGGGGAATTCTAGA+AGG	+	chr8_4:14377574-14377593	Msa1315110:CDS	40.0%
	AATTCCAGGATGGTCATTAG+AGG	-	chr8_4:14377662-14377681	None:intergenic	40.0%
!	GTGTGTCAACCTTAGCATTT+TGG	+	chr8_4:14377719-14377738	Msa1315110:three_prime_UTR	40.0%
!!	CAACCTTAGCATTTTGGTCA+TGG	+	chr8_4:14377725-14377744	Msa1315110:three_prime_UTR	40.0%
	TTCCCATGACCAAAATGCTA+AGG	-	chr8_4:14377731-14377750	None:intergenic	40.0%
	ATGGGTGTACAATTGCTTGA+AGG	-	chr8_4:14377880-14377899	None:intergenic	40.0%
	AAGCAATTGTACACCCATCT+AGG	+	chr8_4:14377882-14377901	Msa1315110:three_prime_UTR	40.0%
!	TTGGATGACAACTTGGGTTG+CGG	-	chr8_4:14373759-14373778	None:intergenic	45.0%
	TTAGAAAAAACGGTGGAGGG+TGG	-	chr8_4:14374227-14374246	None:intergenic	45.0%
	CAGATCGTTGTTCAGCTTCA+GGG	+	chr8_4:14374424-14374443	Msa1315110:CDS	45.0%
	TCACATAACACATGTGGCAG+TGG	+	chr8_4:14375571-14375590	Msa1315110:intron	45.0%
	GGGAGACTTTCTGAACGAAA+TGG	+	chr8_4:14375721-14375740	Msa1315110:CDS	45.0%
!	TGCTATGATAGTTGGTGTGG+TGG	+	chr8_4:14376009-14376028	Msa1315110:intron	45.0%
!	GCTATGATAGTTGGTGTGGT+GGG	+	chr8_4:14376010-14376029	Msa1315110:intron	45.0%
!	CTATGATAGTTGGTGTGGTG+GGG	+	chr8_4:14376011-14376030	Msa1315110:intron	45.0%
!	TATGATAGTTGGTGTGGTGG+GGG	+	chr8_4:14376012-14376031	Msa1315110:intron	45.0%
!	TGTGGTGGGGGTATATTGTT+GGG	+	chr8_4:14376024-14376043	Msa1315110:intron	45.0%
	TATTGTACGAACACACAGCC+CGG	-	chr8_4:14376241-14376260	None:intergenic	45.0%
	AAATGGAGAGGAGAGCTTTG+AGG	+	chr8_4:14376442-14376461	Msa1315110:CDS	45.0%
	AGCACCTCTCTTAATGCTTC+GGG	+	chr8_4:14376518-14376537	Msa1315110:CDS	45.0%
	TCATGACTTTCGGTGAAAGC+GGG	+	chr8_4:14376561-14376580	Msa1315110:CDS	45.0%
	GATGAATTATGGGAAGGCAG+AGG	+	chr8_4:14376610-14376629	Msa1315110:CDS	45.0%
	TGACCCCAAAACAGAAGTTC+TGG	-	chr8_4:14376661-14376680	None:intergenic	45.0%
	CCCATAGGCCAAACAAGTAA+AGG	-	chr8_4:14376803-14376822	None:intergenic	45.0%
!	GAAGCACCTTCAAGAAGATG+TGG	+	chr8_4:14377546-14377565	Msa1315110:CDS	45.0%
	CCTTCTCCACATCTTCTTGA+AGG	-	chr8_4:14377555-14377574	None:intergenic	45.0%
	CCTTCAAGAAGATGTGGAGA+AGG	+	chr8_4:14377552-14377571	Msa1315110:CDS	45.0%
	GATGTGGAGAAGGAAAAAGG+GGG	+	chr8_4:14377562-14377581	Msa1315110:CDS	45.0%
!	AAAGGGGGAATTCTAGAAGG+AGG	+	chr8_4:14377577-14377596	Msa1315110:CDS	45.0%
	GTTTCCTCTAATGACCATCC+TGG	+	chr8_4:14377655-14377674	Msa1315110:CDS	45.0%
	TTAGGAGGTCACAAATGCCT+TGG	+	chr8_4:14377831-14377850	Msa1315110:three_prime_UTR	45.0%
!!!	AAATTATATATTTTTTTTCA+TGG	+	chr8_4:14374271-14374290	Msa1315110:intron	5.0%
!!!	TATTATTTGTTTTTATATAT+TGG	+	chr8_4:14374592-14374611	Msa1315110:intron	5.0%
!!!	ATTATTTGTTTTTATATATT+GGG	+	chr8_4:14374593-14374612	Msa1315110:intron	5.0%
	CCTGAAGCTGAACAACGATC+TGG	-	chr8_4:14374426-14374445	None:intergenic	50.0%
	CCAGATCGTTGTTCAGCTTC+AGG	+	chr8_4:14374423-14374442	Msa1315110:CDS	50.0%
	AGAGGGTCCCAACTCATGAA+TGG	-	chr8_4:14374739-14374758	None:intergenic	50.0%
!	GTGTGGTGGGGGTATATTGT+TGG	+	chr8_4:14376023-14376042	Msa1315110:intron	50.0%
!	GTGGTGGGGGTATATTGTTG+GGG	+	chr8_4:14376025-14376044	Msa1315110:intron	50.0%
!	GCAACCCGAAGCATTAAGAG+AGG	-	chr8_4:14376525-14376544	None:intergenic	50.0%
	ATTGTAGTCGTTGGATCGCC+AGG	+	chr8_4:14376966-14376985	Msa1315110:intron	50.0%
	ATCCTCGACAACAGCAACAC+TGG	-	chr8_4:14377017-14377036	None:intergenic	50.0%
!	CACCAGTGTTGCTGTTGTCG+AGG	+	chr8_4:14377012-14377031	Msa1315110:intron	55.0%
!	GGGGGAATTCTAGAAGGAGG+AGG	+	chr8_4:14377580-14377599	Msa1315110:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	14373611	14378071	14373611	ID=Msa1315110;Name=Msa1315110
chr8_4	mRNA	14373611	14378071	14373611	ID=Msa1315110-mRNA-1;Parent=Msa1315110;Name=Msa1315110-mRNA-1;_AED=0.26;_eAED=0.26;_QI=445\|1\|1\|1\|1\|1\|6\|378\|236
chr8_4	exon	14373611	14374160	14373611	ID=Msa1315110-mRNA-1:exon:5429;Parent=Msa1315110-mRNA-1
chr8_4	exon	14374414	14374507	14374414	ID=Msa1315110-mRNA-1:exon:5430;Parent=Msa1315110-mRNA-1
chr8_4	exon	14374649	14374716	14374649	ID=Msa1315110-mRNA-1:exon:5431;Parent=Msa1315110-mRNA-1
chr8_4	exon	14375716	14375772	14375716	ID=Msa1315110-mRNA-1:exon:5432;Parent=Msa1315110-mRNA-1
chr8_4	exon	14376440	14376676	14376440	ID=Msa1315110-mRNA-1:exon:5433;Parent=Msa1315110-mRNA-1
chr8_4	exon	14377544	14378071	14377544	ID=Msa1315110-mRNA-1:exon:5434;Parent=Msa1315110-mRNA-1
chr8_4	five_prime_UTR	14373611	14374055	14373611	ID=Msa1315110-mRNA-1:five_prime_utr;Parent=Msa1315110-mRNA-1
chr8_4	CDS	14374056	14374160	14374056	ID=Msa1315110-mRNA-1:cds;Parent=Msa1315110-mRNA-1
chr8_4	CDS	14374414	14374507	14374414	ID=Msa1315110-mRNA-1:cds;Parent=Msa1315110-mRNA-1
chr8_4	CDS	14374649	14374716	14374649	ID=Msa1315110-mRNA-1:cds;Parent=Msa1315110-mRNA-1
chr8_4	CDS	14375716	14375772	14375716	ID=Msa1315110-mRNA-1:cds;Parent=Msa1315110-mRNA-1
chr8_4	CDS	14376440	14376676	14376440	ID=Msa1315110-mRNA-1:cds;Parent=Msa1315110-mRNA-1
chr8_4	CDS	14377544	14377693	14377544	ID=Msa1315110-mRNA-1:cds;Parent=Msa1315110-mRNA-1
chr8_4	three_prime_UTR	14377694	14378071	14377694	ID=Msa1315110-mRNA-1:three_prime_utr;Parent=Msa1315110-mRNA-1

Gene Sequence

>Msa1315110

ATGGCTAATGAAGGAAACAAAAGCAATGATTTCTATGCAGTTTTGGGATTGAATAAGGAATGCTCTGATTCAGAACTAAGGAATGCTTATAAGAAACTTGCACTGAAATGGCATCCAGATCGTTGTTCAGCTTCAGGGAATGTGAAGTTTGTGGAAGAAGCTAAGAAGAAATTTCAGGCAATTCAAGAAGCCTATTCTGTTTTATCTGACTCGAACAAGAGATTAATGTACGACGTTGGAGTTTACGACAGTGATGATGACGAAAATGGTATGGGAGACTTTCTGAACGAAATGGTTACAATGATGAGCCAAACTAAATCAAATGAAAATGGAGAGGAGAGCTTTGAGGAGTTGCAACAGTTATTTGATGATATGTTTCAAGCGGATATCGGATTAAATGGAAGCACCTCTCTTAATGCTTCGGGTTGCTCCACTTCATCTACTTTCATGACTTTCGGTGAAAGCGGGAATTCAAATAAGCGCAATTCTACTCAGATGAATTATGGGAAGGCAGAGGATTCTTCTAGTTTTGGTCCAAATTACCAGAACTTCTGTTTTGGGATGAAGCACCTTCAAGAAGATGTGGAGAAGGAAAAAGGGGGAATTCTAGAAGGAGGAGGTAGCAAAAAACAAAGAAATGGAAGAAAACAAAAAATCTCATGTGGACATGATGTTTCCTCTAATGACCATCCTGGAATTTCTGCTAATTGA

Protein sequence

>Msa1315110

MANEGNKSNDFYAVLGLNKECSDSELRNAYKKLALKWHPDRCSASGNVKFVEEAKKKFQAIQEAYSVLSDSNKRLMYDVGVYDSDDDENGMGDFLNEMVTMMSQTKSNENGEESFEELQQLFDDMFQADIGLNGSTSLNASGCSTSSTFMTFGESGNSNKRNSTQMNYGKAEDSSSFGPNYQNFCFGMKHLQEDVEKEKGGILEGGGSKKQRNGRKQKISCGHDVSSNDHPGISAN*