AlfalfaGEDB | Msa1234730

M. sativa cultivar zhongmu-4 / Msa1234730

KEGG: K09512
GO: GO:0003674 GO:0005488 GO:0005515 GO:0051087
PFAM: PF00226.34

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1234730	XP_003627374.1	97.458	236	5	1	1	236	1	235	2.24e-166	468

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1234730	sp\|Q4R7Y5\|DNJB6_MACFA	49.275	69	30	1	10	78	3	66	2.03e-15	76.3

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0006040	Msa1234730	0.886010	4.959487e-72	-8.615850e-47
Msa0013050	Msa1234730	-0.804891	1.737324e-49	-8.615850e-47
Msa0032610	Msa1234730	0.832346	9.960142e-56	-8.615850e-47
Msa0036160	Msa1234730	0.844470	7.410187e-59	-8.615850e-47
Msa0042980	Msa1234730	-0.823229	1.550297e-53	-8.615850e-47
Msa0054750	Msa1234730	0.876136	1.774092e-68	-8.615850e-47
Msa0056030	Msa1234730	0.806855	6.717083e-50	-8.615850e-47
Msa0615270	Msa1234730	-0.808586	2.879870e-50	-8.615850e-47
Msa0629650	Msa1234730	0.822701	2.057875e-53	-8.615850e-47
Msa0650730	Msa1234730	0.806895	6.586486e-50	-8.615850e-47
Msa0654010	Msa1234730	0.815934	7.169322e-52	-8.615850e-47
Msa0661190	Msa1234730	-0.805385	1.369472e-49	-8.615850e-47
Msa0661210	Msa1234730	0.879924	8.376049e-70	-8.615850e-47
Msa0671740	Msa1234730	0.804262	2.350377e-49	-8.615850e-47
Msa0684850	Msa1234730	0.820525	6.551323e-53	-8.615850e-47
Msa0709670	Msa1234730	0.810403	1.173041e-50	-8.615850e-47
Msa0713230	Msa1234730	0.801183	1.015508e-48	-8.615850e-47
Msa0722910	Msa1234730	0.809072	2.266779e-50	-8.615850e-47
Msa0722920	Msa1234730	0.818072	2.371750e-52	-8.615850e-47
Msa0723130	Msa1234730	0.834461	2.957747e-56	-8.615850e-47
Msa0739680	Msa1234730	0.821460	3.990735e-53	-8.615850e-47
Msa0755080	Msa1234730	0.858302	9.041080e-63	-8.615850e-47
Msa0774130	Msa1234730	0.803731	3.030702e-49	-8.615850e-47
Msa0782470	Msa1234730	0.809759	1.614301e-50	-8.615850e-47
Msa0791820	Msa1234730	0.807583	4.709354e-50	-8.615850e-47
Msa0795920	Msa1234730	0.839498	1.528503e-57	-8.615850e-47
Msa0796950	Msa1234730	0.830383	3.028262e-55	-8.615850e-47
Msa0797970	Msa1234730	0.865284	6.601652e-65	-8.615850e-47
Msa0801360	Msa1234730	0.806081	9.783015e-50	-8.615850e-47
Msa0834450	Msa1234730	0.858121	1.023435e-62	-8.615850e-47
Msa0834460	Msa1234730	0.804969	1.673298e-49	-8.615850e-47
Msa0839830	Msa1234730	0.804455	2.142155e-49	-8.615850e-47
Msa0125640	Msa1234730	-0.816977	4.186370e-52	-8.615850e-47
Msa0136890	Msa1234730	-0.817232	3.668724e-52	-8.615850e-47
Msa0148120	Msa1234730	0.849219	3.720540e-60	-8.615850e-47
Msa0170110	Msa1234730	-0.800676	1.288974e-48	-8.615850e-47
Msa0172780	Msa1234730	0.816606	5.071516e-52	-8.615850e-47
Msa0177300	Msa1234730	0.807384	5.189593e-50	-8.615850e-47
Msa0177340	Msa1234730	0.804401	2.199160e-49	-8.615850e-47
Msa0197180	Msa1234730	0.837316	5.585610e-57	-8.615850e-47
Msa0221960	Msa1234730	-0.812573	3.960181e-51	-8.615850e-47
Msa0229490	Msa1234730	-0.803743	3.013037e-49	-8.615850e-47
Msa1234690	Msa1234730	0.977508	6.394284e-144	-8.615850e-47
Msa1234730	Msa1260130	0.816731	4.754380e-52	-8.615850e-47
Msa1234730	Msa1262920	0.810676	1.024077e-50	-8.615850e-47
Msa1234730	Msa1282920	0.810746	9.889246e-51	-8.615850e-47
Msa1234730	Msa1300050	0.803023	4.248705e-49	-8.615850e-47
Msa1234730	Msa1301650	0.847528	1.092724e-59	-8.615850e-47
Msa1234730	Msa1302100	0.831875	1.302272e-55	-8.615850e-47
Msa1234730	Msa1307740	0.834565	2.785345e-56	-8.615850e-47
Msa1234730	Msa1315110	0.995474	1.258567e-216	-8.615850e-47
Msa1234730	Msa1322190	0.812011	5.253310e-51	-8.615850e-47
Msa1234730	Msa1342100	0.801846	7.424463e-49	-8.615850e-47
Msa1234730	Msa1359630	0.880786	4.118321e-70	-8.615850e-47
Msa1234730	Msa1362840	0.813210	2.872814e-51	-8.615850e-47
Msa1234730	Msa1388860	0.815592	8.541296e-52	-8.615850e-47
Msa1234730	Msa1403340	0.823278	1.509377e-53	-8.615850e-47
Msa1234730	Msa1419970	0.832419	9.555879e-56	-8.615850e-47
Msa1234730	Msa1437910	0.801094	1.059116e-48	-8.615850e-47
Msa1234730	Msa1444250	-0.800467	1.421473e-48	-8.615850e-47
Msa1234730	Msa1459760	-0.840095	1.068909e-57	-8.615850e-47
Msa1234730	Msa1465960	0.826981	2.012281e-54	-8.615850e-47
Msa0369720	Msa1234730	-0.809232	2.094762e-50	-8.615850e-47
Msa0379410	Msa1234730	0.842354	2.722299e-58	-8.615850e-47
Msa0380040	Msa1234730	0.812249	4.661906e-51	-8.615850e-47
Msa0389810	Msa1234730	0.836664	8.195521e-57	-8.615850e-47
Msa0394340	Msa1234730	0.835424	1.691722e-56	-8.615850e-47
Msa0429290	Msa1234730	0.808918	2.445111e-50	-8.615850e-47
Msa0442200	Msa1234730	0.821909	3.142734e-53	-8.615850e-47
Msa0457510	Msa1234730	0.828583	8.295713e-55	-8.615850e-47
Msa0468130	Msa1234730	0.842161	3.061249e-58	-8.615850e-47
Msa0475240	Msa1234730	0.823693	1.207117e-53	-8.615850e-47
Msa0475580	Msa1234730	0.819528	1.107652e-52	-8.615850e-47
Msa0479090	Msa1234730	0.808034	3.775592e-50	-8.615850e-47
Msa0982270	Msa1234730	0.836670	8.167670e-57	-8.615850e-47
Msa1012830	Msa1234730	0.813583	2.378796e-51	-8.615850e-47
Msa1012920	Msa1234730	0.812145	4.911718e-51	-8.615850e-47
Msa1021340	Msa1234730	0.813170	2.931635e-51	-8.615850e-47
Msa1037020	Msa1234730	0.807718	4.407701e-50	-8.615850e-47
Msa1054410	Msa1234730	0.845060	5.140397e-59	-8.615850e-47
Msa1077670	Msa1234730	0.836135	1.117382e-56	-8.615850e-47
Msa1079730	Msa1234730	-0.844314	8.161824e-59	-8.615850e-47
Msa1094430	Msa1234730	0.814872	1.235058e-51	-8.615850e-47
Msa0265380	Msa1234730	0.824562	7.548196e-54	-8.615850e-47
Msa0268090	Msa1234730	0.844937	5.548182e-59	-8.615850e-47
Msa0303360	Msa1234730	0.834253	3.334724e-56	-8.615850e-47
Msa0308770	Msa1234730	0.849196	3.777146e-60	-8.615850e-47
Msa0347610	Msa1234730	0.847721	9.663940e-60	-8.615850e-47
Msa1132890	Msa1234730	0.805839	1.099812e-49	-8.615850e-47
Msa1148750	Msa1234730	0.804428	2.170822e-49	-8.615850e-47
Msa1162980	Msa1234730	0.831931	1.262010e-55	-8.615850e-47
Msa1164530	Msa1234730	0.810904	9.140092e-51	-8.615850e-47
Msa1168040	Msa1234730	-0.847869	8.801719e-60	-8.615850e-47
Msa1174870	Msa1234730	0.991468	8.282860e-188	-8.615850e-47
Msa1219810	Msa1234730	0.848237	6.964854e-60	-8.615850e-47
Msa0489610	Msa1234730	0.830487	2.856985e-55	-8.615850e-47
Msa0504410	Msa1234730	0.856540	3.003408e-62	-8.615850e-47
Msa0504450	Msa1234730	0.818350	2.051712e-52	-8.615850e-47
Msa0516160	Msa1234730	0.806365	8.520533e-50	-8.615850e-47
Msa0519220	Msa1234730	0.822866	1.883038e-53	-8.615850e-47
Msa0532540	Msa1234730	0.824071	9.847271e-54	-8.615850e-47
Msa0536650	Msa1234730	0.811220	7.806321e-51	-8.615850e-47
Msa0553220	Msa1234730	-0.807723	4.396851e-50	-8.615850e-47
Msa0561130	Msa1234730	-0.817334	3.480989e-52	-8.615850e-47
Msa0580420	Msa1234730	0.825443	4.675776e-54	-8.615850e-47
Msa0589390	Msa1234730	0.818573	1.826846e-52	-8.615850e-47
Msa0592720	Msa1234730	0.811610	6.422621e-51	-8.615850e-47
Msa0859380	Msa1234730	0.812236	4.692819e-51	-8.615850e-47
Msa0864470	Msa1234730	-0.802232	6.185267e-49	-8.615850e-47
Msa0870150	Msa1234730	0.851217	1.025072e-60	-8.615850e-47
Msa0901630	Msa1234730	0.813737	2.200061e-51	-8.615850e-47
Msa0903400	Msa1234730	0.810493	1.121682e-50	-8.615850e-47
Msa0925660	Msa1234730	0.802957	4.383458e-49	-8.615850e-47
Msa0935850	Msa1234730	0.859768	3.290312e-63	-8.615850e-47
Msa0941540	Msa1234730	0.822026	2.952093e-53	-8.615850e-47
Msa0956410	Msa1234730	0.811221	7.801361e-51	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1234730	MtrunA17_Chr8g0344721	83.628	226	29	2	1	218	1	226	6.76e-134	376
Msa1234730	MtrunA17_Chr7g0271891	41.176	153	80	5	11	162	17	160	1.12e-30	111
Msa1234730	MtrunA17_Chr4g0044331	44.094	127	64	4	16	138	18	141	8.11e-28	103
Msa1234730	MtrunA17_Chr2g0282761	45.122	82	38	2	10	91	4	78	1.94e-15	74.3
Msa1234730	MtrunA17_Chr7g0263971	49.333	75	33	1	6	80	365	434	2.41e-15	74.7
Msa1234730	MtrunA17_Chr2g0324921	49.275	69	30	1	10	78	4	67	4.60e-15	73.2
Msa1234730	MtrunA17_Chr4g0021141	45.455	77	36	1	3	79	15	85	4.66e-15	69.7
Msa1234730	MtrunA17_Chr4g0061421	53.731	67	29	1	12	78	10	74	1.01e-14	72.8
Msa1234730	MtrunA17_Chr4g0006391	46.377	69	32	1	10	78	4	67	4.45e-14	70.5
Msa1234730	MtrunA17_Chr2g0282741	43.478	69	34	1	10	78	4	67	5.63e-14	70.1
Msa1234730	MtrunA17_Chr4g0040501	43.478	69	34	1	10	78	4	67	9.09e-14	69.7
Msa1234730	MtrunA17_Chr2g0295831	45.000	80	37	2	9	88	109	181	2.22e-13	67.4
Msa1234730	MtrunA17_Chr7g0258061	37.273	110	59	3	4	109	16	119	2.48e-13	67.8
Msa1234730	MtrunA17_Chr5g0427221	38.298	94	43	3	7	96	45	127	3.03e-13	65.1
Msa1234730	MtrunA17_Chr3g0138471	43.478	69	34	1	10	78	4	67	3.93e-13	67.8
Msa1234730	MtrunA17_Chr7g0269841	50.000	68	28	2	11	78	27	88	5.08e-12	64.7
Msa1234730	MtrunA17_Chr1g0209551	41.026	78	40	1	9	86	15	86	5.60e-12	64.7
Msa1234730	MtrunA17_Chr8g0378161	44.118	68	34	1	11	78	61	124	7.83e-12	62.4
Msa1234730	MtrunA17_Chr5g0399201	42.857	70	37	1	10	79	12	78	8.75e-12	64.3
Msa1234730	MtrunA17_Chr1g0173931	42.500	80	39	2	8	86	36	109	1.08e-11	63.2
Msa1234730	MtrunA17_Chr2g0295821	41.250	80	40	2	1	80	95	167	3.90e-11	62.0
Msa1234730	MtrunA17_Chr3g0123861	44.928	69	31	1	10	78	82	143	7.08e-11	61.2
Msa1234730	MtrunA17_Chr6g0460381	48.485	66	31	2	10	74	3	66	7.57e-11	59.7
Msa1234730	MtrunA17_Chr6g0460461	47.143	70	34	2	10	78	2	69	7.97e-11	60.1
Msa1234730	MtrunA17_Chr6g0460411	47.143	70	34	2	10	78	2	69	8.26e-11	60.1
Msa1234730	MtrunA17_Chr5g0415731	38.889	72	42	1	9	78	4	75	8.44e-11	58.5
Msa1234730	MtrunA17_Chr7g0269901	40.625	96	44	4	4	88	90	183	9.46e-11	61.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1234730	AT3G14200.1	51.515	231	90	8	10	233	12	227	4.01e-62	194
Msa1234730	AT1G72416.4	63.810	105	25	2	10	109	5	101	2.28e-40	137
Msa1234730	AT1G72416.2	65.049	103	23	2	10	107	5	99	2.40e-40	137
Msa1234730	AT1G72416.1	65.049	103	23	2	10	107	5	99	3.30e-40	137
Msa1234730	AT1G72416.3	65.049	103	23	2	10	107	5	99	8.61e-40	136
Msa1234730	AT1G56300.1	50.376	133	57	4	1	129	1	128	7.92e-33	117
Msa1234730	AT1G71000.1	48.611	144	58	6	4	139	2	137	9.47e-33	117
Msa1234730	AT1G71000.2	42.593	162	79	5	4	159	2	155	5.06e-28	105
Msa1234730	AT1G56300.2	51.818	110	47	3	1	106	1	108	8.15e-28	103
Msa1234730	AT1G72416.5	73.469	49	13	0	61	109	10	58	9.88e-19	80.5
Msa1234730	AT5G01390.3	49.275	69	30	1	10	78	4	67	4.19e-15	72.4
Msa1234730	AT5G01390.2	49.275	69	30	1	10	78	4	67	7.94e-15	72.4
Msa1234730	AT1G59725.1	46.377	69	33	1	10	78	4	68	1.06e-14	72.4
Msa1234730	AT5G01390.1	49.275	69	30	1	10	78	4	67	1.28e-14	72.4
Msa1234730	AT5G01390.4	49.275	69	30	1	10	78	4	67	2.30e-14	70.5
Msa1234730	AT5G49060.2	46.914	81	35	3	7	86	96	169	2.50e-14	71.6
Msa1234730	AT5G49060.1	46.914	81	35	3	7	86	96	169	2.50e-14	71.6
Msa1234730	AT5G03160.1	46.667	75	35	1	6	80	366	435	4.40e-14	71.2
Msa1234730	AT5G25530.2	44.928	69	33	1	10	78	4	67	5.79e-14	70.5
Msa1234730	AT4G28480.2	46.377	69	32	1	10	78	4	67	7.92e-14	69.7
Msa1234730	AT3G08910.1	46.377	69	32	1	10	78	4	67	9.22e-14	69.7
Msa1234730	AT4G28480.1	46.377	69	32	1	10	78	4	67	1.36e-13	69.3
Msa1234730	AT2G35720.1	42.308	78	42	1	2	79	7	81	2.15e-13	69.3
Msa1234730	AT3G47940.1	42.857	70	36	1	10	79	4	69	2.26e-13	68.9
Msa1234730	AT1G10350.1	42.029	69	35	1	10	78	4	67	4.01e-13	68.2
Msa1234730	AT5G16650.1	39.024	82	43	2	3	83	4	79	6.34e-13	64.3
Msa1234730	AT2G20560.1	44.928	69	33	1	10	78	4	67	8.73e-13	67.0
Msa1234730	AT5G16650.2	39.024	82	43	2	3	83	4	79	2.38e-12	63.5
Msa1234730	AT3G12170.1	38.793	116	61	4	1	113	1	109	3.01e-12	65.1
Msa1234730	AT2G33735.1	50.704	71	25	2	10	78	22	84	3.05e-12	62.0
Msa1234730	AT2G33735.2	50.704	71	25	2	10	78	22	84	3.05e-12	62.0
Msa1234730	AT3G12170.2	37.931	116	62	4	1	113	37	145	7.05e-12	64.3
Msa1234730	AT5G06910.1	35.556	135	54	5	1	130	19	125	1.07e-11	63.5
Msa1234730	AT5G25530.1	40.260	77	33	2	10	78	4	75	2.11e-11	63.2
Msa1234730	AT4G39960.1	39.785	93	43	3	9	97	84	167	2.55e-11	63.2
Msa1234730	AT4G39960.2	39.785	93	43	3	9	97	84	167	2.55e-11	63.2
Msa1234730	AT1G80030.4	42.254	71	34	1	8	78	73	136	4.74e-11	62.4
Msa1234730	AT1G80030.2	42.254	71	34	1	8	78	73	136	6.24e-11	62.0
Msa1234730	AT1G80030.3	42.254	71	34	1	8	78	73	136	6.24e-11	62.0
Msa1234730	AT1G80030.1	42.254	71	34	1	8	78	73	136	6.24e-11	62.0
Msa1234730	AT3G62600.1	48.529	68	29	2	11	78	27	88	8.37e-11	61.2

Find 69 sgRNAs with CRISPR-Local

Find 266 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAGAAGTTCTGGTAATT+TGG	0.142853	8_2:+12399226	None:intergenic
AATTCTACTCAGATGAATTA+TGG	0.200136	8_2:-12399277	Msa1234730:CDS
TCTAGTTGGTTGTGAAAATT+TGG	0.205516	8_2:-12401881	Msa1234730:five_prime_UTR
TGTTATTGTTGGAAATAATA+TGG	0.247286	8_2:-12401856	Msa1234730:exon
ATTAGTCATTTGTTGCATTA+TGG	0.247313	8_2:-12397872	Msa1234730:three_prime_UTR
GAAGATGTGGAGAAGGAAAA+AGG	0.260664	8_2:-12398317	Msa1234730:CDS
CCTTCTCCACATCTTCTTGA+AGG	0.267807	8_2:+12398324	None:intergenic
GTGTGTCAACCTTAGCATTT+TGG	0.273693	8_2:-12398157	Msa1234730:three_prime_UTR
CAATGATTTCTATGCAGTTT+TGG	0.279803	8_2:-12401814	Msa1234730:CDS
CCAGATCGTTGTTCAGCTTC+AGG	0.283002	8_2:-12401440	Msa1234730:CDS
AATTACCAGAACTTCTGTTT+TGG	0.283009	8_2:-12399223	Msa1234730:CDS
GGCTCACATTTGATTTAGTT+TGG	0.297419	8_2:+12400119	None:intergenic
CAAGCGGATATCGGATTAAA+TGG	0.298600	8_2:-12399382	Msa1234730:CDS
GCAGAGGATTCTTCTAGTTT+TGG	0.305479	8_2:-12399250	Msa1234730:CDS
AGCACCTCTCTTAATGCTTC+GGG	0.329574	8_2:-12399358	Msa1234730:CDS
CTTAAACTCAAACTCCTAGA+TGG	0.329984	8_2:+12397980	None:intergenic
CTTCAACTTGGATGACAACT+TGG	0.330506	8_2:+12402130	None:intergenic
CTAATGTTTCTTTGTATTGT+CGG	0.332006	8_2:+12401952	None:intergenic
TTATATGTGATACAGGAAAA+TGG	0.333099	8_2:-12399451	Msa1234730:intron
TATATAAAGTCTAGAATTAG+AGG	0.347820	8_2:-12402263	Msa1234730:five_prime_UTR
GCAATTCAAGAAGCCTATTC+TGG	0.348966	8_2:-12401377	Msa1234730:intron
AGAAGCTAAGAAGAAATTTC+AGG	0.357982	8_2:-12401399	Msa1234730:CDS
TCATCTACTTTCATGACTTT+CGG	0.362653	8_2:-12399325	Msa1234730:CDS
AAATGCCTTGGAAAATTTGA+TGG	0.373884	8_2:-12398033	Msa1234730:three_prime_UTR
GTTTGGTTGGTTGATCTAGT+TGG	0.390684	8_2:-12401895	Msa1234730:five_prime_UTR
AAGCACCTCTCTTAATGCTT+CGG	0.391365	8_2:-12399359	Msa1234730:CDS
GATATGTTTCAAGCGGATAT+CGG	0.410739	8_2:-12399391	Msa1234730:CDS
TCTGTTGGAGAATTCTAGTT+TGG	0.411390	8_2:-12401912	Msa1234730:five_prime_UTR
ACATTAGTTTGATATTTGAT+TGG	0.420967	8_2:-12401936	Msa1234730:five_prime_UTR
ATTCTACTCAGATGAATTAT+GGG	0.423184	8_2:-12399276	Msa1234730:CDS
TGACCCCAAAACAGAAGTTC+TGG	0.428777	8_2:+12399218	None:intergenic
AAAATTTGGATTGTTATTGT+TGG	0.435217	8_2:-12401867	Msa1234730:five_prime_UTR
CAGATCGTTGTTCAGCTTCA+GGG	0.447334	8_2:-12401439	Msa1234730:CDS
AAGCAATTGTACACCCATCT+AGG	0.450605	8_2:-12397994	Msa1234730:three_prime_UTR
TGCATGTAAATTACCAGAAT+AGG	0.452286	8_2:+12401364	None:intergenic
AAATGGAGAGGAGAGCTTTG+AGG	0.455188	8_2:-12399434	Msa1234730:CDS
TATCAATTAGCAGAAATTCC+AGG	0.462490	8_2:+12398203	None:intergenic
CCTGAAGCTGAACAACGATC+TGG	0.468892	8_2:+12401440	None:intergenic
AATTCCAGGATGGTCATTAG+AGG	0.478304	8_2:+12398217	None:intergenic
GGGAGACTTTCTGAACGAAA+TGG	0.482099	8_2:-12400155	Msa1234730:CDS
TTGGATGACAACTTGGGTTG+CGG	0.483218	8_2:+12402137	None:intergenic
TACTAAGATAAGATAAACTA+TGG	0.485649	8_2:+12398077	None:intergenic
GGAAATAATATGGCTAATGA+AGG	0.487826	8_2:-12401846	Msa1234730:exon
TTGGAGAATTCTAGTTTGGT+TGG	0.496008	8_2:-12401908	Msa1234730:five_prime_UTR
TCATGACTTTCGGTGAAAGC+GGG	0.503824	8_2:-12399315	Msa1234730:CDS
TTCCCATGACCAAAATGCTA+AGG	0.508517	8_2:+12398148	None:intergenic
TGATATTTGATTGGTTCTGT+TGG	0.511150	8_2:-12401927	Msa1234730:five_prime_UTR
AATGCTCTGATTCAGAACTA+AGG	0.513112	8_2:-12401779	Msa1234730:CDS
TTCAGGGAATGTGAAGTTTG+TGG	0.516942	8_2:-12401423	Msa1234730:CDS
ATGGGTGTACAATTGCTTGA+AGG	0.522891	8_2:+12397999	None:intergenic
TTAGGAGGTCACAAATGCCT+TGG	0.526911	8_2:-12398045	Msa1234730:three_prime_UTR
AGTCATGAAAGTAGATGAAG+TGG	0.538936	8_2:+12399330	None:intergenic
TGCTTATAAGAAACTTGCAC+TGG	0.543386	8_2:-12401754	Msa1234730:intron
TTCAACTTGGATGACAACTT+GGG	0.545053	8_2:+12402131	None:intergenic
ATTAATATACAACTTCAACT+TGG	0.554791	8_2:+12402118	None:intergenic
TTAAACTCAAACTCCTAGAT+GGG	0.559329	8_2:+12397981	None:intergenic
GTTTCCTCTAATGACCATCC+TGG	0.561659	8_2:-12398221	Msa1234730:CDS
ATTTGATGATATGTTTCAAG+CGG	0.571158	8_2:-12399398	Msa1234730:CDS
TTTAGACTAGATGGAATGAA+GGG	0.573998	8_2:+12402201	None:intergenic
TTCATGACTTTCGGTGAAAG+CGG	0.579461	8_2:-12399316	Msa1234730:CDS
CCTTCAAGAAGATGTGGAGA+AGG	0.586502	8_2:-12398324	Msa1234730:CDS
GAAGCACCTTCAAGAAGATG+TGG	0.587778	8_2:-12398330	Msa1234730:CDS
GATGAATTATGGGAAGGCAG+AGG	0.603296	8_2:-12399266	Msa1234730:CDS
TACTCAGATGAATTATGGGA+AGG	0.609702	8_2:-12399272	Msa1234730:CDS
TGTGATACAGGAAAATGGAG+AGG	0.651222	8_2:-12399446	Msa1234730:intron
AAGAGATTAATGTACGACGT+TGG	0.670458	8_2:-12401197	Msa1234730:CDS
AATTAGCAGAAATTCCAGGA+TGG	0.683865	8_2:+12398207	None:intergenic
GCAACCCGAAGCATTAAGAG+AGG	0.689028	8_2:+12399354	None:intergenic
AGGTCTCTAGAGTATTCATG+AGG	0.690314	8_2:-12402243	Msa1234730:five_prime_UTR

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAATTAGATATTTTTTTTCA+TGG	-	chr8_2:12398471-12398490	Msa1234730:intron	10.0%
!!	AAGAAAATAATTTGATTAAA+TGG	-	chr8_2:12398633-12398652	Msa1234730:intron	10.0%
!!	TTGATTATATGAAAAAAAAT+CGG	+	chr8_2:12399545-12399564	None:intergenic	10.0%
!!	TATTGCAAAAATATTAAATA+GGG	+	chr8_2:12400014-12400033	None:intergenic	10.0%
!!	ATATTGCAAAAATATTAAAT+AGG	+	chr8_2:12400015-12400034	None:intergenic	10.0%
!!!	TTTATATATTTTTTGTTTAG+TGG	-	chr8_2:12400111-12400130	Msa1234730:intron	10.0%
!!	TATTTAAAAAATAATTCAAG+TGG	-	chr8_2:12400351-12400370	Msa1234730:intron	10.0%
!!	ACAAATAATAATTTGAATCA+TGG	+	chr8_2:12398828-12398847	None:intergenic	15.0%
!!!	TTCTCAATCTATTTTTTAAA+CGG	+	chr8_2:12399260-12399279	None:intergenic	15.0%
!!	TGATTATATGAAAAAAAATC+GGG	+	chr8_2:12399544-12399563	None:intergenic	15.0%
!!!	AACAAGATATTATTTTATTG+TGG	-	chr8_2:12400392-12400411	Msa1234730:intron	15.0%
!!!	CTTTAAATTATACAAGATTT+TGG	-	chr8_2:12401113-12401132	Msa1234730:intron	15.0%
!!!	TATATAAAGTCTAGAATTAG+AGG	-	chr8_2:12397846-12397865	Msa1234730:three_prime_UTR	20.0%
!!	ATTAATATACAACTTCAACT+TGG	+	chr8_2:12397994-12398013	None:intergenic	20.0%
!!!	GTTTCTTACTTTTTATGTTA+AGG	-	chr8_2:12398089-12398108	Msa1234730:three_prime_UTR	20.0%
!!!	ACGAATTTTGTGTTTTTATT+TGG	-	chr8_2:12398116-12398135	Msa1234730:three_prime_UTR	20.0%
!!!	TTTTGTGTTTTTATTTGGTT+TGG	-	chr8_2:12398121-12398140	Msa1234730:three_prime_UTR	20.0%
!!!	ACATTAGTTTGATATTTGAT+TGG	-	chr8_2:12398173-12398192	Msa1234730:three_prime_UTR	20.0%
!!!	AAAATTTGGATTGTTATTGT+TGG	-	chr8_2:12398242-12398261	Msa1234730:CDS	20.0%
!!	TGTTATTGTTGGAAATAATA+TGG	-	chr8_2:12398253-12398272	Msa1234730:CDS	20.0%
!!!	GAATAAACTTTAGAAAAAAC+AGG	+	chr8_2:12398436-12398455	None:intergenic	20.0%
!!!	TTTTCTAAAGTTTATTCACA+TGG	-	chr8_2:12398439-12398458	Msa1234730:intron	20.0%
!!!	TTTCTAAAGTTTATTCACAT+GGG	-	chr8_2:12398440-12398459	Msa1234730:intron	20.0%
!!!	AATGTAAATGGTGTTTTTTT+TGG	-	chr8_2:12398867-12398886	Msa1234730:intron	20.0%
!!!	ATGTAAATGGTGTTTTTTTT+GGG	-	chr8_2:12398868-12398887	Msa1234730:intron	20.0%
!!	AAAGTGAAAAGGTAAAATTA+AGG	+	chr8_2:12399087-12399106	None:intergenic	20.0%
!!	ATTCAGTTTCAATATTCATT+AGG	+	chr8_2:12399139-12399158	None:intergenic	20.0%
!!	ATGAATATTGAAACTGAATT+AGG	-	chr8_2:12399140-12399159	Msa1234730:intron	20.0%
!!	TGAATATTGAAACTGAATTA+GGG	-	chr8_2:12399141-12399160	Msa1234730:intron	20.0%
!!!	TGGAAATGAGTTAATTTTTT+AGG	-	chr8_2:12399360-12399379	Msa1234730:CDS	20.0%
!!!	GTTTAAAATAGTGACTTAAT+TGG	+	chr8_2:12399422-12399441	None:intergenic	20.0%
!!	TTATAGCACATCAAAATTAT+TGG	+	chr8_2:12399902-12399921	None:intergenic	20.0%
!!!	AAAATGAATTTTGTTTTGCA+GGG	-	chr8_2:12399928-12399947	Msa1234730:intron	20.0%
!!	AACAAGACATAAACATTATT+AGG	+	chr8_2:12400213-12400232	None:intergenic	20.0%
!!!	AATAATGTTTATGTCTTGTT+TGG	-	chr8_2:12400213-12400232	Msa1234730:intron	20.0%
!!	CAAGATTTGAAAATAATTGT+TGG	-	chr8_2:12400426-12400445	Msa1234730:intron	20.0%
!!	ATTTGAAAATAATTGTTGGA+AGG	-	chr8_2:12400430-12400449	Msa1234730:intron	20.0%
!!!	AATCAATTTATGAAAAGTAC+AGG	+	chr8_2:12400535-12400554	None:intergenic	20.0%
!!!	AGTTAAAAGGATTGAAATTT+TGG	+	chr8_2:12400597-12400616	None:intergenic	20.0%
!!	AATGAATTGAAAGAGTTAAA+AGG	+	chr8_2:12400610-12400629	None:intergenic	20.0%
!!	GATTAATTTATATGTGATAC+AGG	-	chr8_2:12400651-12400670	Msa1234730:intron	20.0%
!!	TTTAACATGTAATTGAGTTA+TGG	-	chr8_2:12401084-12401103	Msa1234730:intron	20.0%
!!	TTAACATGTAATTGAGTTAT+GGG	-	chr8_2:12401085-12401104	Msa1234730:intron	20.0%
!!!	ATGATAGAAGATTTAGAATT+TGG	-	chr8_2:12401146-12401165	Msa1234730:intron	20.0%
!!	ACTCTATTAATAACTCAAAA+TGG	+	chr8_2:12401588-12401607	None:intergenic	20.0%
!!	TTTGTATCAAATTCTCAAAT+TGG	+	chr8_2:12401617-12401636	None:intergenic	20.0%
!!!	TGCAATTTTTTTAAGAAGAT+TGG	-	chr8_2:12401695-12401714	Msa1234730:intron	20.0%
!!!	TCCAACATTTTTTTTATTCT+TGG	-	chr8_2:12401743-12401762	Msa1234730:intron	20.0%
!!	ACCAAGAATAAAAAAAATGT+TGG	+	chr8_2:12401747-12401766	None:intergenic	20.0%
!!	ATGTACAACTAAAAAGATTA+GGG	-	chr8_2:12402003-12402022	Msa1234730:five_prime_UTR	20.0%
!!	TACTAAGATAAGATAAACTA+TGG	+	chr8_2:12402035-12402054	None:intergenic	20.0%
!!	ATCTTAGTACTAAAAAGATT+AGG	-	chr8_2:12402046-12402065	Msa1234730:five_prime_UTR	20.0%
!!!	AATGTCAATTTTGATTTGAA+AGG	-	chr8_2:12402270-12402289	Msa1234730:five_prime_UTR	20.0%
!	CTAATGTTTCTTTGTATTGT+CGG	+	chr8_2:12398160-12398179	None:intergenic	25.0%
!!	AATGATTTCTATGCAGTTTT+GGG	-	chr8_2:12398296-12398315	Msa1234730:CDS	25.0%
!!	AAACTTTAGAAAAAACAGGA+TGG	+	chr8_2:12398432-12398451	None:intergenic	25.0%
!	GTTATTCACAATCAAATTGA+GGG	+	chr8_2:12398506-12398525	None:intergenic	25.0%
!	TATCTTCCTTTCACAAATTT+AGG	+	chr8_2:12398563-12398582	None:intergenic	25.0%
!!	TTTGTGAAAGGAAGATATTT+TGG	-	chr8_2:12398566-12398585	Msa1234730:intron	25.0%
!	ATTGTTGATATGATGAAAGT+TGG	-	chr8_2:12398598-12398617	Msa1234730:intron	25.0%
!	TTTGATTAAATGGACAGAAA+TGG	-	chr8_2:12398643-12398662	Msa1234730:intron	25.0%
!!	TATTGGGTCTAAAATGTAAA+TGG	-	chr8_2:12398855-12398874	Msa1234730:intron	25.0%
!	CATAACACAAAAATAGAAGT+AGG	+	chr8_2:12399022-12399041	None:intergenic	25.0%
!!	TTGTTTCTTCATTTACTTGA+AGG	-	chr8_2:12399044-12399063	Msa1234730:intron	25.0%
!	TGTTTCTTCATTTACTTGAA+GGG	-	chr8_2:12399045-12399064	Msa1234730:intron	25.0%
!	TTGTTAATGTGAAAGTGAAA+AGG	+	chr8_2:12399098-12399117	None:intergenic	25.0%
!!	CACTTTCACATTAACAATTT+TGG	-	chr8_2:12399101-12399120	Msa1234730:intron	25.0%
!!	TATAAGGATGATTTGATAGT+TGG	-	chr8_2:12399165-12399184	Msa1234730:intron	25.0%
!	AACAATGATATGGTCAATTT+CGG	-	chr8_2:12399315-12399334	Msa1234730:CDS	25.0%
!	CAACGAAAACAAGAAAAAAA+TGG	-	chr8_2:12399340-12399359	Msa1234730:CDS	25.0%
!!!	AGTCACTATTTTAAACCTAA+AGG	-	chr8_2:12399426-12399445	Msa1234730:CDS	25.0%
!	AAGAGGAGAAGAAAAATAAT+TGG	+	chr8_2:12399648-12399667	None:intergenic	25.0%
!	AAAAAGAGATATGTTCTTCA+AGG	-	chr8_2:12399676-12399695	Msa1234730:intron	25.0%
!!!	AACATTTTTTTTCCTCCTTT+GGG	+	chr8_2:12399738-12399757	None:intergenic	25.0%
!!!	AAACATTTTTTTTCCTCCTT+TGG	+	chr8_2:12399739-12399758	None:intergenic	25.0%
!	GATTCAATTCCATGAATTAT+TGG	+	chr8_2:12399858-12399877	None:intergenic	25.0%
!	ATTCATGGAATTGAATCAAA+TGG	-	chr8_2:12399861-12399880	Msa1234730:intron	25.0%
!!!	GAAAATGAATTTTGTTTTGC+AGG	-	chr8_2:12399927-12399946	Msa1234730:intron	25.0%
!!!	TTTTGTTTGCTTGTAATTAG+TGG	-	chr8_2:12400042-12400061	Msa1234730:intron	25.0%
!	TTATATGTGATACAGGAAAA+TGG	-	chr8_2:12400658-12400677	Msa1234730:intron	25.0%
!!	ATTTGATGATATGTTTCAAG+CGG	-	chr8_2:12400711-12400730	Msa1234730:intron	25.0%
!	AATTCTACTCAGATGAATTA+TGG	-	chr8_2:12400832-12400851	Msa1234730:intron	25.0%
!	ATTCTACTCAGATGAATTAT+GGG	-	chr8_2:12400833-12400852	Msa1234730:intron	25.0%
!	TTGAATGATTGTTTATTGTG+AGG	-	chr8_2:12401009-12401028	Msa1234730:intron	25.0%
!	AGTAGTAACAACAAAAAGAT+AGG	-	chr8_2:12401061-12401080	Msa1234730:intron	25.0%
!	TAACATGTAATTGAGTTATG+GGG	-	chr8_2:12401086-12401105	Msa1234730:intron	25.0%
!	TAATCTATCTTCAGAGTAAT+TGG	+	chr8_2:12401314-12401333	None:intergenic	25.0%
!	TTAAGTGGTTAAGGAAAAAA+TGG	-	chr8_2:12401331-12401350	Msa1234730:intron	25.0%
!	CACTAATTAAAAGACTCATT+GGG	+	chr8_2:12401379-12401398	None:intergenic	25.0%
!!!	TTTTGTTGTTCATGTTATCA+AGG	-	chr8_2:12401406-12401425	Msa1234730:CDS	25.0%
!	ATTATCATTACCAATGAATG+AGG	-	chr8_2:12401441-12401460	Msa1234730:CDS	25.0%
!	TTACAAAACTAATAGAGTCA+GGG	-	chr8_2:12401529-12401548	Msa1234730:intron	25.0%
!	AGAAAACAAAAAATCTCATG+TGG	-	chr8_2:12401858-12401877	Msa1234730:exon	25.0%
!	CATGTACAACTAAAAAGATT+AGG	-	chr8_2:12402002-12402021	Msa1234730:five_prime_UTR	25.0%
!	TGTACAACTAAAAAGATTAG+GGG	-	chr8_2:12402004-12402023	Msa1234730:five_prime_UTR	25.0%
!	TTAGTACTAAAAAGATTAGG+AGG	-	chr8_2:12402049-12402068	Msa1234730:five_prime_UTR	25.0%
!!	ATTAGTCATTTGTTGCATTA+TGG	-	chr8_2:12402237-12402256	Msa1234730:five_prime_UTR	25.0%
	TTTAGACTAGATGGAATGAA+GGG	+	chr8_2:12397911-12397930	None:intergenic	30.0%
!	TTTTAGACTAGATGGAATGA+AGG	+	chr8_2:12397912-12397931	None:intergenic	30.0%
!!!	GCATTTTGTTTTAGACTAGA+TGG	+	chr8_2:12397920-12397939	None:intergenic	30.0%
!!	TGATATTTGATTGGTTCTGT+TGG	-	chr8_2:12398182-12398201	Msa1234730:three_prime_UTR	30.0%
	TCTAGTTGGTTGTGAAAATT+TGG	-	chr8_2:12398228-12398247	Msa1234730:CDS	30.0%
	GGAAATAATATGGCTAATGA+AGG	-	chr8_2:12398263-12398282	Msa1234730:CDS	30.0%
!	CAATGATTTCTATGCAGTTT+TGG	-	chr8_2:12398295-12398314	Msa1234730:CDS	30.0%
	GGTTATTCACAATCAAATTG+AGG	+	chr8_2:12398507-12398526	None:intergenic	30.0%
!	ACTTTCATGGTACACAATTT+TGG	+	chr8_2:12398528-12398547	None:intergenic	30.0%
	GACTCAAAATGTAACTTTCA+TGG	+	chr8_2:12398541-12398560	None:intergenic	30.0%
	TTGAGTCCTAAATTTGTGAA+AGG	-	chr8_2:12398554-12398573	Msa1234730:intron	30.0%
	AGAAGCTAAGAAGAAATTTC+AGG	-	chr8_2:12398710-12398729	Msa1234730:intron	30.0%
	TGCATGTAAATTACCAGAAT+AGG	+	chr8_2:12398748-12398767	None:intergenic	30.0%
	TAATTTGAATCATGGCTCAT+GGG	+	chr8_2:12398820-12398839	None:intergenic	30.0%
	ATAATTTGAATCATGGCTCA+TGG	+	chr8_2:12398821-12398840	None:intergenic	30.0%
	GTTTCAATATTCATTAGGCA+TGG	+	chr8_2:12399134-12399153	None:intergenic	30.0%
	GAAACTGAATTAGGGATATA+AGG	-	chr8_2:12399149-12399168	Msa1234730:intron	30.0%
	TTAAAGATCATAGTGATGAG+CGG	-	chr8_2:12399196-12399215	Msa1234730:intron	30.0%
	GGAGAAGAAAAATAATTGGA+CGG	+	chr8_2:12399644-12399663	None:intergenic	30.0%
	ATATGTTCTTCAAGGTTAGT+AGG	-	chr8_2:12399684-12399703	Msa1234730:intron	30.0%
	TTTGGGAAAAGACATTTGTT+GGG	+	chr8_2:12399721-12399740	None:intergenic	30.0%
	CAATAAGAGCCAATAATTCA+TGG	-	chr8_2:12399846-12399865	Msa1234730:intron	30.0%
!!!	AATTTTGTTTTGCAGGGTAT+GGG	-	chr8_2:12399934-12399953	Msa1234730:intron	30.0%
	TAATTGTTGGAAGGATAAAG+TGG	-	chr8_2:12400439-12400458	Msa1234730:intron	30.0%
	TCATCTACTTTCATGACTTT+CGG	-	chr8_2:12400784-12400803	Msa1234730:intron	30.0%
!	AAACAGAAGTTCTGGTAATT+TGG	+	chr8_2:12400886-12400905	None:intergenic	30.0%
!	AATTACCAGAACTTCTGTTT+TGG	-	chr8_2:12400886-12400905	Msa1234730:intron	30.0%
!	ATTACCAGAACTTCTGTTTT+GGG	-	chr8_2:12400887-12400906	Msa1234730:intron	30.0%
	ATGAATGTTGCATCAAATGA+AGG	+	chr8_2:12400947-12400966	None:intergenic	30.0%
	TTCATTTGATGCAACATTCA+TGG	-	chr8_2:12400946-12400965	Msa1234730:intron	30.0%
!	TACTCTGAAGATAGATTAAG+TGG	-	chr8_2:12401316-12401335	Msa1234730:intron	30.0%
	GAAGATAGATTAAGTGGTTA+AGG	-	chr8_2:12401322-12401341	Msa1234730:intron	30.0%
	CCACTAATTAAAAGACTCAT+TGG	+	chr8_2:12401380-12401399	None:intergenic	30.0%
!!	CCAATGAGTCTTTTAATTAG+TGG	-	chr8_2:12401377-12401396	Msa1234730:intron	30.0%
!	TGTTTTGTATCCTCATTCAT+TGG	+	chr8_2:12401454-12401473	None:intergenic	30.0%
	TGAATGAGGATACAAAACAA+TGG	-	chr8_2:12401455-12401474	Msa1234730:intron	30.0%
!!!	CTGACTCTATTAGTTTTGTA+AGG	+	chr8_2:12401530-12401549	None:intergenic	30.0%
	CTTACAAAACTAATAGAGTC+AGG	-	chr8_2:12401528-12401547	Msa1234730:intron	30.0%
	AAAGACAGAGAGTAATTTGA+TGG	-	chr8_2:12401634-12401653	Msa1234730:intron	30.0%
	CACTCCATATATTATATAGC+TGG	-	chr8_2:12401659-12401678	Msa1234730:intron	30.0%
	GAGACCAGCTATATAATATA+TGG	+	chr8_2:12401666-12401685	None:intergenic	30.0%
	CAGTATCAAGTTAAATGTAG+TGG	+	chr8_2:12401722-12401741	None:intergenic	30.0%
	GGTAGCAAAAAACAAAGAAA+TGG	-	chr8_2:12401834-12401853	Msa1234730:CDS	30.0%
	TATCAATTAGCAGAAATTCC+AGG	+	chr8_2:12401909-12401928	None:intergenic	30.0%
	GTACAACTAAAAAGATTAGG+GGG	-	chr8_2:12402005-12402024	Msa1234730:five_prime_UTR	30.0%
	AAATGCCTTGGAAAATTTGA+TGG	-	chr8_2:12402076-12402095	Msa1234730:five_prime_UTR	30.0%
	TTAAACTCAAACTCCTAGAT+GGG	+	chr8_2:12402131-12402150	None:intergenic	30.0%
	TTCAACTTGGATGACAACTT+GGG	+	chr8_2:12397981-12398000	None:intergenic	35.0%
!	TCTGTTGGAGAATTCTAGTT+TGG	-	chr8_2:12398197-12398216	Msa1234730:exon	35.0%
!!	TTGGAGAATTCTAGTTTGGT+TGG	-	chr8_2:12398201-12398220	Msa1234730:exon	35.0%
!!!	TGCAGTTTTGGGATTGAATA+AGG	-	chr8_2:12398307-12398326	Msa1234730:CDS	35.0%
	AATGCTCTGATTCAGAACTA+AGG	-	chr8_2:12398330-12398349	Msa1234730:CDS	35.0%
	TGCTTATAAGAAACTTGCAC+TGG	-	chr8_2:12398355-12398374	Msa1234730:intron	35.0%
	GGTTAGAATAGAAACAGAGA+CGG	+	chr8_2:12398411-12398430	None:intergenic	35.0%
	AAGAGATTAATGTACGACGT+TGG	-	chr8_2:12398912-12398931	Msa1234730:intron	35.0%
	ATTTCACACCATTCATGAGT+TGG	-	chr8_2:12398974-12398993	Msa1234730:intron	35.0%
	TTTCACACCATTCATGAGTT+GGG	-	chr8_2:12398975-12398994	Msa1234730:intron	35.0%
!	AAGGATGATTTGATAGTTGG+TGG	-	chr8_2:12399168-12399187	Msa1234730:intron	35.0%
!	AGGATGATTTGATAGTTGGT+GGG	-	chr8_2:12399169-12399188	Msa1234730:intron	35.0%
	TAAAGATCATAGTGATGAGC+GGG	-	chr8_2:12399197-12399216	Msa1234730:intron	35.0%
	TGAGAATGAATTTCCTCTCT+TGG	-	chr8_2:12399274-12399293	Msa1234730:CDS	35.0%
	TTGACCATATCATTGTTTCG+TGG	+	chr8_2:12399312-12399331	None:intergenic	35.0%
	TCACTGTCTTCTTTACCTTT+AGG	+	chr8_2:12399444-12399463	None:intergenic	35.0%
!!!	TCTCTTTTTTCACGTGAAAG+AGG	+	chr8_2:12399665-12399684	None:intergenic	35.0%
!	TTGGGAAAAGACATTTGTTG+GGG	+	chr8_2:12399720-12399739	None:intergenic	35.0%
!	CTTTGGGAAAAGACATTTGT+TGG	+	chr8_2:12399722-12399741	None:intergenic	35.0%
!	CAACAAATGTCTTTTCCCAA+AGG	-	chr8_2:12399720-12399739	Msa1234730:intron	35.0%
	TATGTGTCACATAACACATG+TGG	-	chr8_2:12399798-12399817	Msa1234730:intron	35.0%
	CATGGAATTGAATCAAATGG+AGG	-	chr8_2:12399864-12399883	Msa1234730:intron	35.0%
!!!	GAATTTTGTTTTGCAGGGTA+TGG	-	chr8_2:12399933-12399952	Msa1234730:intron	35.0%
!	GGCTCACATTTGATTTAGTT+TGG	+	chr8_2:12399993-12400012	None:intergenic	35.0%
	CTTGTAATTAGTGGAACGTT+AGG	-	chr8_2:12400051-12400070	Msa1234730:intron	35.0%
	AATTAGTGGAACGTTAGGTT+AGG	-	chr8_2:12400056-12400075	Msa1234730:intron	35.0%
	AGGCACAACTACATAGTATT+AGG	+	chr8_2:12400169-12400188	None:intergenic	35.0%
!	AGTTGTGCCTTTTTACCTTT+TGG	-	chr8_2:12400179-12400198	Msa1234730:intron	35.0%
!	GTTGTGCCTTTTTACCTTTT+GGG	-	chr8_2:12400180-12400199	Msa1234730:intron	35.0%
!!!	CCTTTTTACCTTTTGGGATT+GGG	-	chr8_2:12400186-12400205	Msa1234730:intron	35.0%
	ACACCAACTATCATAGCAAT+TGG	+	chr8_2:12400240-12400259	None:intergenic	35.0%
!	AATTGCTATGATAGTTGGTG+TGG	-	chr8_2:12400239-12400258	Msa1234730:intron	35.0%
	GATATGTTTCAAGCGGATAT+CGG	-	chr8_2:12400718-12400737	Msa1234730:intron	35.0%
	AGTCATGAAAGTAGATGAAG+TGG	+	chr8_2:12400782-12400801	None:intergenic	35.0%
	TACTCAGATGAATTATGGGA+AGG	-	chr8_2:12400837-12400856	Msa1234730:intron	35.0%
!	TTACCAGAACTTCTGTTTTG+GGG	-	chr8_2:12400888-12400907	Msa1234730:intron	35.0%
	GGCAAACATATAGCATAGTA+AGG	-	chr8_2:12400967-12400986	Msa1234730:intron	35.0%
	TGTTACTACTTACTACCCAT+AGG	+	chr8_2:12401051-12401070	None:intergenic	35.0%
	ACAACAATTCATGCTTCAGA+TGG	+	chr8_2:12401273-12401292	None:intergenic	35.0%
	TATCTTCAGAGTAATTGGCA+AGG	+	chr8_2:12401309-12401328	None:intergenic	35.0%
	ACAAAACAATGGAGTATGTG+TGG	-	chr8_2:12401466-12401485	Msa1234730:intron	35.0%
	ATGTGTGGAGTATATAGAAG+TGG	-	chr8_2:12401481-12401500	Msa1234730:intron	35.0%
!!	AGTTTTGTAAGGAGATCACT+AGG	+	chr8_2:12401519-12401538	None:intergenic	35.0%
	TTGTCAAACGTTTCTCAGAT+TGG	+	chr8_2:12401556-12401575	None:intergenic	35.0%
	AAGATGTGGAGAAGGAAAAA+GGG	-	chr8_2:12401793-12401812	Msa1234730:CDS	35.0%
	AATTAGCAGAAATTCCAGGA+TGG	+	chr8_2:12401905-12401924	None:intergenic	35.0%
!!	AACCTTAGCATTTTGGTCAT+GGG	-	chr8_2:12401959-12401978	Msa1234730:five_prime_UTR	35.0%
!	CACTACCATCAAATTTTCCA+AGG	+	chr8_2:12402084-12402103	None:intergenic	35.0%
	CTTAAACTCAAACTCCTAGA+TGG	+	chr8_2:12402132-12402151	None:intergenic	35.0%
!!	AGGTCTCTAGAGTATTCATG+AGG	-	chr8_2:12397866-12397885	Msa1234730:three_prime_UTR	40.0%
	CTTCAACTTGGATGACAACT+TGG	+	chr8_2:12397982-12398001	None:intergenic	40.0%
!	GTTTGGTTGGTTGATCTAGT+TGG	-	chr8_2:12398214-12398233	Msa1234730:CDS	40.0%
	TTCAGGGAATGTGAAGTTTG+TGG	-	chr8_2:12398686-12398705	Msa1234730:intron	40.0%
	GCAATTCAAGAAGCCTATTC+TGG	-	chr8_2:12398732-12398751	Msa1234730:intron	40.0%
	AGGCAAGTGAAAGAGAAAGA+GGG	+	chr8_2:12399002-12399021	None:intergenic	40.0%
	TAGGCAAGTGAAAGAGAAAG+AGG	+	chr8_2:12399003-12399022	None:intergenic	40.0%
!	GGATGATTTGATAGTTGGTG+GGG	-	chr8_2:12399170-12399189	Msa1234730:intron	40.0%
	GAAGCAAATATGACCAAGAG+AGG	+	chr8_2:12399290-12399309	None:intergenic	40.0%
	GCTTCCACGAAACAATGATA+TGG	-	chr8_2:12399305-12399324	Msa1234730:CDS	40.0%
!	CAAATGTCTTTTCCCAAAGG+AGG	-	chr8_2:12399723-12399742	Msa1234730:intron	40.0%
!!!	GCCTTTTTACCTTTTGGGAT+TGG	-	chr8_2:12400185-12400204	Msa1234730:intron	40.0%
	CCCAATCCCAAAAGGTAAAA+AGG	+	chr8_2:12400189-12400208	None:intergenic	40.0%
	TATTAGGACCCAATCCCAAA+AGG	+	chr8_2:12400197-12400216	None:intergenic	40.0%
	GGTCCAATTGCTATGATAGT+TGG	-	chr8_2:12400234-12400253	Msa1234730:intron	40.0%
	GGAAGGATAAAGTGGTTTAC+TGG	-	chr8_2:12400447-12400466	Msa1234730:intron	40.0%
	GATAAAGTGGTTTACTGGAC+CGG	-	chr8_2:12400452-12400471	Msa1234730:intron	40.0%
	ATAAAGTGGTTTACTGGACC+GGG	-	chr8_2:12400453-12400472	Msa1234730:intron	40.0%
	TGTGATACAGGAAAATGGAG+AGG	-	chr8_2:12400663-12400682	Msa1234730:intron	40.0%
	CAAGCGGATATCGGATTAAA+TGG	-	chr8_2:12400727-12400746	Msa1234730:intron	40.0%
	AAGCACCTCTCTTAATGCTT+CGG	-	chr8_2:12400750-12400769	Msa1234730:intron	40.0%
	TTCATGACTTTCGGTGAAAG+CGG	-	chr8_2:12400793-12400812	Msa1234730:intron	40.0%
!!!	GCAGAGGATTCTTCTAGTTT+TGG	-	chr8_2:12400859-12400878	Msa1234730:intron	40.0%
	TGTGAGGTCCTTTACTTGTT+TGG	-	chr8_2:12401025-12401044	Msa1234730:intron	40.0%
	TCCTTTACTTGTTTGGCCTA+TGG	-	chr8_2:12401032-12401051	Msa1234730:intron	40.0%
	CCTTTACTTGTTTGGCCTAT+GGG	-	chr8_2:12401033-12401052	Msa1234730:intron	40.0%
	GATGCAACTATTGTAGTCGT+TGG	-	chr8_2:12401190-12401209	Msa1234730:CDS	40.0%
!	AATGCACATTTGTTGGATCC+TGG	+	chr8_2:12401220-12401239	None:intergenic	40.0%
	TGCAACGAATGCACATTTGT+TGG	+	chr8_2:12401227-12401246	None:intergenic	40.0%
	GAAGATGTGGAGAAGGAAAA+AGG	-	chr8_2:12401792-12401811	Msa1234730:CDS	40.0%
	AGATGTGGAGAAGGAAAAAG+GGG	-	chr8_2:12401794-12401813	Msa1234730:CDS	40.0%
	GAAAAAGGGGGAATTCTAGA+AGG	-	chr8_2:12401807-12401826	Msa1234730:CDS	40.0%
	AATTCCAGGATGGTCATTAG+AGG	+	chr8_2:12401895-12401914	None:intergenic	40.0%
!	GTGTGTCAACCTTAGCATTT+TGG	-	chr8_2:12401952-12401971	Msa1234730:five_prime_UTR	40.0%
!!	CAACCTTAGCATTTTGGTCA+TGG	-	chr8_2:12401958-12401977	Msa1234730:five_prime_UTR	40.0%
	TTCCCATGACCAAAATGCTA+AGG	+	chr8_2:12401964-12401983	None:intergenic	40.0%
	ATGGGTGTACAATTGCTTGA+AGG	+	chr8_2:12402113-12402132	None:intergenic	40.0%
	AAGCAATTGTACACCCATCT+AGG	-	chr8_2:12402115-12402134	Msa1234730:five_prime_UTR	40.0%
!	TTGGATGACAACTTGGGTTG+CGG	+	chr8_2:12397975-12397994	None:intergenic	45.0%
	CAGATCGTTGTTCAGCTTCA+GGG	-	chr8_2:12398670-12398689	Msa1234730:intron	45.0%
	TCACATAACACATGTGGCAG+TGG	-	chr8_2:12399804-12399823	Msa1234730:intron	45.0%
	GGGAGACTTTCTGAACGAAA+TGG	-	chr8_2:12399954-12399973	Msa1234730:intron	45.0%
!	TGCTATGATAGTTGGTGTGG+TGG	-	chr8_2:12400242-12400261	Msa1234730:intron	45.0%
!	GCTATGATAGTTGGTGTGGT+GGG	-	chr8_2:12400243-12400262	Msa1234730:intron	45.0%
!	CTATGATAGTTGGTGTGGTG+GGG	-	chr8_2:12400244-12400263	Msa1234730:intron	45.0%
!	TATGATAGTTGGTGTGGTGG+GGG	-	chr8_2:12400245-12400264	Msa1234730:intron	45.0%
!	TGTGGTGGGGGTATATTGTT+GGG	-	chr8_2:12400257-12400276	Msa1234730:intron	45.0%
	TATTGTACGAACACACAGCC+CGG	+	chr8_2:12400474-12400493	None:intergenic	45.0%
	AAATGGAGAGGAGAGCTTTG+AGG	-	chr8_2:12400675-12400694	Msa1234730:intron	45.0%
	AGCACCTCTCTTAATGCTTC+GGG	-	chr8_2:12400751-12400770	Msa1234730:intron	45.0%
	TCATGACTTTCGGTGAAAGC+GGG	-	chr8_2:12400794-12400813	Msa1234730:intron	45.0%
	GATGAATTATGGGAAGGCAG+AGG	-	chr8_2:12400843-12400862	Msa1234730:intron	45.0%
	TGACCCCAAAACAGAAGTTC+TGG	+	chr8_2:12400894-12400913	None:intergenic	45.0%
	CCCATAGGCCAAACAAGTAA+AGG	+	chr8_2:12401036-12401055	None:intergenic	45.0%
!	GAAGCACCTTCAAGAAGATG+TGG	-	chr8_2:12401779-12401798	Msa1234730:CDS	45.0%
	CCTTCTCCACATCTTCTTGA+AGG	+	chr8_2:12401788-12401807	None:intergenic	45.0%
	CCTTCAAGAAGATGTGGAGA+AGG	-	chr8_2:12401785-12401804	Msa1234730:CDS	45.0%
	GATGTGGAGAAGGAAAAAGG+GGG	-	chr8_2:12401795-12401814	Msa1234730:CDS	45.0%
!	AAAGGGGGAATTCTAGAAGG+AGG	-	chr8_2:12401810-12401829	Msa1234730:CDS	45.0%
	GTTTCCTCTAATGACCATCC+TGG	-	chr8_2:12401888-12401907	Msa1234730:five_prime_UTR	45.0%
	TTAGGAGGTCACAAATGCCT+TGG	-	chr8_2:12402064-12402083	Msa1234730:five_prime_UTR	45.0%
!!!	TATTATTTGTTTTTATATAT+TGG	-	chr8_2:12398838-12398857	Msa1234730:intron	5.0%
!!!	ATTATTTGTTTTTATATATT+GGG	-	chr8_2:12398839-12398858	Msa1234730:intron	5.0%
	CCTGAAGCTGAACAACGATC+TGG	+	chr8_2:12398672-12398691	None:intergenic	50.0%
	CCAGATCGTTGTTCAGCTTC+AGG	-	chr8_2:12398669-12398688	Msa1234730:intron	50.0%
	AGAGGGTCCCAACTCATGAA+TGG	+	chr8_2:12398985-12399004	None:intergenic	50.0%
!	GTGTGGTGGGGGTATATTGT+TGG	-	chr8_2:12400256-12400275	Msa1234730:intron	50.0%
!	GTGGTGGGGGTATATTGTTG+GGG	-	chr8_2:12400258-12400277	Msa1234730:intron	50.0%
!	GCAACCCGAAGCATTAAGAG+AGG	+	chr8_2:12400758-12400777	None:intergenic	50.0%
	ATTGTAGTCGTTGGATCGCC+AGG	-	chr8_2:12401199-12401218	Msa1234730:CDS	50.0%
	ATCCTCGACAACAGCAACAC+TGG	+	chr8_2:12401250-12401269	None:intergenic	50.0%
!	CACCAGTGTTGCTGTTGTCG+AGG	-	chr8_2:12401245-12401264	Msa1234730:intron	55.0%
!	GGGGGAATTCTAGAAGGAGG+AGG	-	chr8_2:12401813-12401832	Msa1234730:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	12397827	12402304	12397827	ID=Msa1234730;Name=Msa1234730
chr8_2	mRNA	12397827	12402304	12397827	ID=Msa1234730-mRNA-1;Parent=Msa1234730;Name=Msa1234730-mRNA-1;_AED=0.26;_eAED=0.26;_QI=445\|1\|1\|1\|1\|1\|6\|378\|236
chr8_2	exon	12397827	12398354	12397827	ID=Msa1234730-mRNA-1:exon:4815;Parent=Msa1234730-mRNA-1
chr8_2	exon	12399222	12399458	12399222	ID=Msa1234730-mRNA-1:exon:4814;Parent=Msa1234730-mRNA-1
chr8_2	exon	12400126	12400182	12400126	ID=Msa1234730-mRNA-1:exon:4813;Parent=Msa1234730-mRNA-1
chr8_2	exon	12401169	12401236	12401169	ID=Msa1234730-mRNA-1:exon:4812;Parent=Msa1234730-mRNA-1
chr8_2	exon	12401378	12401471	12401378	ID=Msa1234730-mRNA-1:exon:4811;Parent=Msa1234730-mRNA-1
chr8_2	exon	12401755	12402304	12401755	ID=Msa1234730-mRNA-1:exon:4810;Parent=Msa1234730-mRNA-1
chr8_2	five_prime_UTR	12401860	12402304	12401860	ID=Msa1234730-mRNA-1:five_prime_utr;Parent=Msa1234730-mRNA-1
chr8_2	CDS	12401755	12401859	12401755	ID=Msa1234730-mRNA-1:cds;Parent=Msa1234730-mRNA-1
chr8_2	CDS	12401378	12401471	12401378	ID=Msa1234730-mRNA-1:cds;Parent=Msa1234730-mRNA-1
chr8_2	CDS	12401169	12401236	12401169	ID=Msa1234730-mRNA-1:cds;Parent=Msa1234730-mRNA-1
chr8_2	CDS	12400126	12400182	12400126	ID=Msa1234730-mRNA-1:cds;Parent=Msa1234730-mRNA-1
chr8_2	CDS	12399222	12399458	12399222	ID=Msa1234730-mRNA-1:cds;Parent=Msa1234730-mRNA-1
chr8_2	CDS	12398205	12398354	12398205	ID=Msa1234730-mRNA-1:cds;Parent=Msa1234730-mRNA-1
chr8_2	three_prime_UTR	12397827	12398204	12397827	ID=Msa1234730-mRNA-1:three_prime_utr;Parent=Msa1234730-mRNA-1

Gene Sequence

>Msa1234730

ATGGCTAATGAAGGAAACAAAAGCAATGATTTCTATGCAGTTTTGGGATTGAATAAGGAATGCTCTGATTCAGAACTAAGGAATGCTTATAAGAAACTTGCACTGAAATGGCATCCAGATCGTTGTTCAGCTTCAGGGAATGTGAAGTTTGTGGAAGAAGCTAAGAAGAAATTTCAGGCAATTCAAGAAGCCTATTCTGTTTTATCTGACTCGAACAAGAGATTAATGTACGACGTTGGAGTTTACGACAGTGATGATGACGAAAATGGTATGGGAGACTTTCTGAACGAAATGGTTACAATGATGAGCCAAACTAAATCAAATGAAAATGGAGAGGAGAGCTTTGAGGAGTTGCAACAGTTATTTGATGATATGTTTCAAGCGGATATCGGATTAAATGGAAGCACCTCTCTTAATGCTTCGGGTTGCTCCACTTCATCTACTTTCATGACTTTCGGTGAAAGCGGGAATTCAAATAAGCGCAATTCTACTCAGATGAATTATGGGAAGGCAGAGGATTCTTCTAGTTTTGGTCCAAATTACCAGAACTTCTGTTTTGGGATGAAGCACCTTCAAGAAGATGTGGAGAAGGAAAAAGGGGGAATTCTAGAAGGAGGAGGTAGCAAAAAACAAAGAAATGGAAGAAAACAAAAAATCTCATGTGGACATGATGTTTCCTCTAATGACCATCCTGGAATTTCTGCTAATTGA

Protein sequence

>Msa1234730

MANEGNKSNDFYAVLGLNKECSDSELRNAYKKLALKWHPDRCSASGNVKFVEEAKKKFQAIQEAYSVLSDSNKRLMYDVGVYDSDDDENGMGDFLNEMVTMMSQTKSNENGEESFEELQQLFDDMFQADIGLNGSTSLNASGCSTSSTFMTFGESGNSNKRNSTQMNYGKAEDSSSFGPNYQNFCFGMKHLQEDVEKEKGGILEGGGSKKQRNGRKQKISCGHDVSSNDHPGISAN*