AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1447110


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1447110 MtrunA17_Chr1g0210761 98.095 210 4 0 1 210 1 210 2.88e-151 418
Msa1447110 MtrunA17_Chr1g0178711 56.373 204 78 1 17 209 20 223 5.98e-82 243
Msa1447110 MtrunA17_Chr1g0193671 42.381 210 114 3 7 209 7 216 2.89e-53 170
Msa1447110 MtrunA17_Chr7g0271211 44.898 196 99 4 22 209 19 213 2.46e-52 167
Msa1447110 MtrunA17_Chr1g0193421 43.434 198 105 3 19 209 20 217 1.79e-51 165
Msa1447110 MtrunA17_Chr6g0449751 43.721 215 105 6 7 207 3 215 1.14e-47 155
Msa1447110 MtrunA17_Chr6g0449731 43.981 216 106 6 5 207 2 215 2.16e-47 155
Msa1447110 MtrunA17_Chr1g0189841 44.059 202 98 6 19 207 18 217 3.81e-47 154
Msa1447110 MtrunA17_Chr6g0449771 44.700 217 103 8 5 207 2 215 4.71e-47 154
Msa1447110 MtrunA17_Chr6g0449761 43.721 215 108 5 5 207 2 215 4.89e-47 155
Msa1447110 MtrunA17_Chr4g0007031 41.627 209 90 7 19 206 18 215 1.57e-43 145
Msa1447110 MtrunA17_Chr5g0419971 41.627 209 90 7 19 206 18 215 1.57e-43 145
Msa1447110 MtrunA17_Chr5g0419981 41.627 209 90 7 19 206 18 215 1.57e-43 145
Msa1447110 MtrunA17_Chr4g0007051 41.546 207 93 7 19 206 18 215 1.59e-43 145
Msa1447110 MtrunA17_Chr4g0007041 40.179 224 97 8 9 206 3 215 2.24e-43 145
Msa1447110 MtrunA17_Chr2g0285871 41.627 209 90 7 19 206 18 215 3.97e-43 144
Msa1447110 MtrunA17_Chr1g0156131 39.109 202 116 4 11 207 10 209 7.83e-43 143
Msa1447110 MtrunA17_Chr5g0414971 41.753 194 105 4 22 207 26 219 8.38e-43 143
Msa1447110 MtrunA17_Chr2g0292831 40.513 195 108 5 20 207 19 212 3.48e-42 141
Msa1447110 MtrunA17_Chr2g0292901 40.957 188 103 5 27 207 27 213 7.29e-42 140
Msa1447110 MtrunA17_Chr6g0449781 40.196 204 100 6 19 207 18 214 7.01e-38 130
Msa1447110 MtrunA17_Chr2g0300591 36.946 203 119 3 9 204 3 203 2.12e-37 129
Msa1447110 MtrunA17_Chr2g0300581 36.765 204 120 3 9 205 3 204 2.18e-37 129
Msa1447110 MtrunA17_Chr2g0292861 41.860 172 94 4 42 207 7 178 4.59e-37 127
Msa1447110 MtrunA17_Chr2g0300561 36.816 201 122 2 9 204 3 203 6.41e-37 128
Msa1447110 MtrunA17_Chr8g0343911 36.058 208 115 5 10 205 1 202 8.96e-36 125
Msa1447110 MtrunA17_Chr2g0292841 41.279 172 95 4 42 207 7 178 1.07e-35 124
Msa1447110 MtrunA17_Chr2g0313961 34.783 207 124 5 8 207 3 205 1.85e-35 124
Msa1447110 MtrunA17_Chr3g0084531 34.300 207 125 5 8 207 3 205 8.25e-35 122
Msa1447110 MtrunA17_Chr8g0343934 35.437 206 124 6 8 207 3 205 3.06e-34 121
Msa1447110 MtrunA17_Chr8g0344041 33.014 209 131 5 5 207 4 209 3.15e-34 121
Msa1447110 MtrunA17_Chr2g0297231 33.816 207 126 5 8 207 3 205 3.18e-34 120
Msa1447110 MtrunA17_Chr3g0130241 41.765 170 87 2 15 172 75 244 9.63e-34 122
Msa1447110 MtrunA17_Chr2g0321601 34.286 210 112 5 12 208 8 204 1.40e-33 119
Msa1447110 MtrunA17_Chr8g0343921 32.367 207 129 5 8 207 1 203 4.12e-33 118
Msa1447110 MtrunA17_Chr2g0297241 33.333 207 127 5 8 207 3 205 5.04e-33 118
Msa1447110 MtrunA17_Chr6g0449791 39.109 202 108 5 7 197 9 206 5.89e-33 117
Msa1447110 MtrunA17_Chr2g0302171 36.517 178 106 4 33 207 32 205 6.12e-33 117
Msa1447110 MtrunA17_Chr3g0084521 35.266 207 119 6 8 207 6 204 1.03e-32 117
Msa1447110 MtrunA17_Chr8g0343954 37.433 187 108 6 27 207 22 205 1.13e-32 117
Msa1447110 MtrunA17_Chr3g0084511 35.749 207 118 7 8 207 6 204 4.02e-32 115
Msa1447110 MtrunA17_Chr8g0335851 35.294 187 113 2 22 200 25 211 7.75e-32 115
Msa1447110 MtrunA17_Chr2g0301611 32.850 207 128 5 8 207 3 205 1.11e-31 114
Msa1447110 MtrunA17_Chr8g0343961 33.990 203 125 5 11 207 6 205 2.31e-31 113
Msa1447110 MtrunA17_Chr4g0003361 33.163 196 122 4 16 207 11 201 2.85e-31 113
Msa1447110 MtrunA17_Chr4g0026191 33.019 212 122 5 5 208 7 206 2.39e-30 110
Msa1447110 MtrunA17_Chr8g0344021 33.173 208 128 4 8 207 4 208 4.67e-30 110
Msa1447110 MtrunA17_Chr2g0321581 31.731 208 120 5 12 208 9 205 7.33e-30 109
Msa1447110 MtrunA17_Chr2g0321591 31.731 208 120 5 12 208 9 205 7.57e-30 109
Msa1447110 MtrunA17_Chr4g0026201 37.500 160 96 2 53 208 47 206 1.64e-29 108
Msa1447110 MtrunA17_Chr2g0302541 34.574 188 112 5 27 207 22 205 2.79e-28 105
Msa1447110 MtrunA17_Chr3g0115141 34.595 185 110 5 29 207 27 206 8.94e-28 104
Msa1447110 MtrunA17_Chr4g0012651 33.511 188 114 5 27 207 22 205 1.53e-27 103
Msa1447110 MtrunA17_Chr7g0217811 31.351 185 122 2 27 207 22 205 2.79e-27 103
Msa1447110 MtrunA17_Chr2g0292911 45.299 117 61 2 42 155 7 123 5.62e-27 100
Msa1447110 MtrunA17_Chr5g0411961 30.435 207 133 5 8 207 3 205 5.99e-27 102
Msa1447110 MtrunA17_Chr8g0344051 30.097 206 137 3 8 207 4 208 8.21e-27 102
Msa1447110 MtrunA17_Chr8g0344031 32.857 210 125 8 8 207 4 207 8.55e-27 102
Msa1447110 MtrunA17_Chr2g0286171 32.258 186 113 7 29 207 29 208 1.95e-24 95.9
Msa1447110 MtrunA17_Chr3g0084551 29.064 203 129 5 8 204 3 196 6.12e-21 87.0
Msa1447110 MtrunA17_Chr3g0084501 34.906 106 59 4 8 106 3 105 3.17e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1447110 AT3G10080.1 60.194 206 70 3 13 209 19 221 1.56e-86 255
Msa1447110 AT3G10080.2 60.194 206 70 3 13 209 90 292 6.09e-86 256
Msa1447110 AT1G02335.1 42.326 215 116 4 1 207 1 215 7.56e-56 177
Msa1447110 AT3G04200.1 45.070 213 104 5 8 209 14 224 1.12e-53 172
Msa1447110 AT1G18980.1 45.192 208 105 4 7 207 13 218 9.39e-53 169
Msa1447110 AT1G18970.1 42.308 208 111 4 7 207 13 218 1.09e-51 166
Msa1447110 AT5G26700.1 40.865 208 116 4 7 209 6 211 1.98e-51 165
Msa1447110 AT3G62020.1 43.204 206 109 4 12 209 12 217 2.90e-51 165
Msa1447110 AT5G39160.3 41.346 208 112 5 7 207 11 215 1.28e-50 163
Msa1447110 AT5G39160.1 40.845 213 110 6 7 207 11 219 1.48e-50 163
Msa1447110 AT5G39130.1 40.376 213 111 6 7 207 11 219 2.42e-50 163
Msa1447110 AT5G39190.1 40.845 213 110 6 7 207 11 219 2.58e-50 163
Msa1447110 AT1G09560.1 44.103 195 102 3 22 209 23 217 3.53e-50 162
Msa1447110 AT5G38960.1 44.059 202 99 5 19 207 22 222 5.20e-50 162
Msa1447110 AT3G05950.1 43.925 214 105 6 7 208 12 222 9.26e-50 162
Msa1447110 AT3G05930.1 38.710 217 125 3 1 209 1 217 8.84e-49 159
Msa1447110 AT5G39110.1 41.014 217 113 6 5 207 5 220 2.48e-48 158
Msa1447110 AT5G39180.1 40.845 213 112 5 8 207 8 219 9.32e-48 156
Msa1447110 AT5G38910.1 42.326 215 109 6 6 207 7 219 1.06e-47 156
Msa1447110 AT3G62020.2 45.699 186 95 4 28 209 5 188 1.48e-47 155
Msa1447110 AT5G38940.1 42.424 198 101 5 22 207 25 221 1.56e-47 155
Msa1447110 AT5G38940.2 42.424 198 101 5 22 207 49 245 2.17e-47 156
Msa1447110 AT5G39150.1 40.845 213 112 5 8 207 8 219 2.34e-47 155
Msa1447110 AT5G39120.1 40.639 219 113 6 5 207 2 219 5.88e-47 154
Msa1447110 AT5G39160.2 39.130 207 100 6 7 207 11 197 4.42e-45 149
Msa1447110 AT5G39190.2 39.130 207 100 6 7 207 11 197 6.53e-45 148
Msa1447110 AT3G04180.1 39.500 200 110 4 20 208 23 222 9.18e-45 149
Msa1447110 AT4G14630.1 39.500 200 107 5 21 207 24 222 1.96e-44 148
Msa1447110 AT3G04170.1 41.315 213 111 6 8 210 13 221 2.43e-44 148
Msa1447110 AT3G04150.1 39.623 212 115 5 8 208 13 222 6.23e-44 147
Msa1447110 AT5G38930.1 40.201 199 106 5 21 207 24 221 2.19e-43 145
Msa1447110 AT3G04190.1 40.299 201 107 5 20 208 23 222 2.79e-43 145
Msa1447110 AT3G04150.2 38.222 225 113 6 8 208 13 235 2.63e-42 143
Msa1447110 AT5G20630.1 38.798 183 107 2 27 204 22 204 1.63e-39 135
Msa1447110 AT1G10460.1 36.181 199 113 5 22 207 18 215 6.69e-39 133
Msa1447110 AT5G61750.1 38.983 177 101 3 9 178 5 181 2.05e-34 122
Msa1447110 AT1G74820.1 31.390 223 134 6 2 208 7 226 3.89e-33 119
Msa1447110 AT1G72610.1 36.508 189 103 3 27 204 19 201 4.37e-33 118
Msa1447110 AT5G38910.2 35.681 213 89 6 6 207 7 182 5.52e-31 112
Msa1447110 AT5G38950.1 49.206 63 30 1 138 198 2 64 3.05e-12 61.2

Find 20 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGATTAAAGATGAGCTTT+TGG 0.263245 tig0031598:-9634 Msa1447110:CDS
GAATTTGTTATCTTCTGATT+TGG 0.267937 tig0031598:+10082 None:intergenic
CCAGTATCTTTGAAGTTTCC+TGG 0.322734 tig0031598:+9999 None:intergenic
TTCTGATCCGGATCCTGTTA+TGG 0.323739 tig0031598:-10117 Msa1447110:CDS
GTGAATTCAAATGTGTTTCC+AGG 0.358885 tig0031598:-9960 Msa1447110:CDS
CCAGGAAACTTCAAAGATAC+TGG 0.364740 tig0031598:-9999 Msa1447110:CDS
TTTGGAGAAAGGTGAGGTTA+TGG 0.393881 tig0031598:-9778 Msa1447110:CDS
TGAAAATGCACAAGTCCTCT+TGG 0.414763 tig0031598:+9750 None:intergenic
GCTATGCAGTAATCCATAAC+AGG 0.434646 tig0031598:+10104 None:intergenic
ATAGCAACTTCTGTTGCTCT+TGG 0.444865 tig0031598:+9864 None:intergenic
CAGTAATCCATAACAGGATC+CGG 0.469062 tig0031598:+10110 None:intergenic
TTTCCAGGTTTAAACACTCT+TGG 0.469660 tig0031598:-9945 Msa1447110:CDS
AGAAGTTGCTATTGTGTTGG+AGG 0.523101 tig0031598:-9853 Msa1447110:CDS
AACAGAAGTTGCTATTGTGT+TGG 0.541553 tig0031598:-9856 Msa1447110:CDS
ACACATAAGAACTAAAAGAA+TGG 0.546948 tig0031598:+10160 None:intergenic
GAAGTTGCTATTGTGTTGGA+GGG 0.567803 tig0031598:-9852 Msa1447110:CDS
ACTCCAAGAGTGTTTAAACC+TGG 0.567991 tig0031598:+9942 None:intergenic
GAAATCTTCAACTGTTGCTG+TGG 0.571728 tig0031598:+10043 None:intergenic
AGCTTTCTAAGTTAAAGAAG+AGG 0.576087 tig0031598:-9584 Msa1447110:CDS
GTTGCTCTTGGATGAAAGTG+AGG 0.618955 tig0031598:+9876 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATTCCAAATGTTGTTTT+TGG - tig0031598:10088-10107 Msa1447110:CDS 20.0%
! ACACATAAGAACTAAAAGAA+TGG + tig0031598:9591-9610 None:intergenic 25.0%
!!! GAATTTGTTATCTTCTGATT+TGG + tig0031598:9669-9688 None:intergenic 25.0%
!! CAAGATTTTTGCTAAAGTTT+TGG - tig0031598:9952-9971 Msa1447110:CDS 25.0%
!!! GTTGAAGATTTCACTTTTTC+TGG - tig0031598:9716-9735 Msa1447110:CDS 30.0%
!! TTTTCTGGTATAAAACTACC+AGG - tig0031598:9731-9750 Msa1447110:CDS 30.0%
! AGATACTGGATTTTCTTCAA+TGG - tig0031598:9763-9782 Msa1447110:CDS 30.0%
! GATACTGGATTTTCTTCAAT+GGG - tig0031598:9764-9783 Msa1447110:CDS 30.0%
TTGGAGGGAAAAATTTATTC+AGG - tig0031598:9911-9930 Msa1447110:CDS 30.0%
!!! TTTGCTAAAGTTTTGGAGAA+AGG - tig0031598:9959-9978 Msa1447110:CDS 30.0%
AAACTACCAAAAATAGTAGC+AGG + tig0031598:10043-10062 None:intergenic 30.0%
!! AGTTTTGATAGTCAAAATCC+TGG - tig0031598:10058-10077 Msa1447110:CDS 30.0%
!! TTTGGAATTTTCATCAAACC+AGG + tig0031598:10079-10098 None:intergenic 30.0%
TGAGATCCAAAAACAACATT+TGG + tig0031598:10097-10116 None:intergenic 30.0%
! TCAGATTAAAGATGAGCTTT+TGG - tig0031598:10114-10133 Msa1447110:CDS 30.0%
! AGCTTTTGGACTTAATTCTA+AGG - tig0031598:10141-10160 Msa1447110:CDS 30.0%
AGCTTTCTAAGTTAAAGAAG+AGG - tig0031598:10164-10183 Msa1447110:CDS 30.0%
GTGAATTCAAATGTGTTTCC+AGG - tig0031598:9788-9807 Msa1447110:CDS 35.0%
TTTCCAGGTTTAAACACTCT+TGG - tig0031598:9803-9822 Msa1447110:CDS 35.0%
!! GTTAGAGCAGATTTTGATGT+TGG - tig0031598:9836-9855 Msa1447110:CDS 35.0%
! AACAGAAGTTGCTATTGTGT+TGG - tig0031598:9892-9911 Msa1447110:CDS 35.0%
!!! TAAAGTTTTGGAGAAAGGTG+AGG - tig0031598:9964-9983 Msa1447110:CDS 35.0%
! GTGCATTTTCAGATGAATGT+TGG - tig0031598:10010-10029 Msa1447110:CDS 35.0%
! GATGTTCCTGCTACTATTTT+TGG - tig0031598:10034-10053 Msa1447110:CDS 35.0%
!! GAGCTTTTGGAAAAAGCTTT+TGG - tig0031598:10127-10146 Msa1447110:CDS 35.0%
!!! TGCTGCTTTTTGTTCTGATC+CGG - tig0031598:9619-9638 Msa1447110:CDS 40.0%
CAGTAATCCATAACAGGATC+CGG + tig0031598:9641-9660 None:intergenic 40.0%
GCTATGCAGTAATCCATAAC+AGG + tig0031598:9647-9666 None:intergenic 40.0%
GAAATCTTCAACTGTTGCTG+TGG + tig0031598:9708-9727 None:intergenic 40.0%
!! CCAGTATCTTTGAAGTTTCC+TGG + tig0031598:9752-9771 None:intergenic 40.0%
CCAGGAAACTTCAAAGATAC+TGG - tig0031598:9749-9768 Msa1447110:CDS 40.0%
!! ACTCCAAGAGTGTTTAAACC+TGG + tig0031598:9809-9828 None:intergenic 40.0%
!! AGAGCAGATTTTGATGTTGG+TGG - tig0031598:9839-9858 Msa1447110:CDS 40.0%
ATAGCAACTTCTGTTGCTCT+TGG + tig0031598:9887-9906 None:intergenic 40.0%
! AGAAGTTGCTATTGTGTTGG+AGG - tig0031598:9895-9914 Msa1447110:CDS 40.0%
! GAAGTTGCTATTGTGTTGGA+GGG - tig0031598:9896-9915 Msa1447110:CDS 40.0%
TTTGGAGAAAGGTGAGGTTA+TGG - tig0031598:9970-9989 Msa1447110:CDS 40.0%
! GAGGTTATGGTTTTTCCAAG+AGG - tig0031598:9983-10002 Msa1447110:CDS 40.0%
TGAAAATGCACAAGTCCTCT+TGG + tig0031598:10001-10020 None:intergenic 40.0%
TTCTGATCCGGATCCTGTTA+TGG - tig0031598:9631-9650 Msa1447110:CDS 45.0%
GTTGCTCTTGGATGAAAGTG+AGG + tig0031598:9875-9894 None:intergenic 45.0%
Chromosome Type Strat End Strand Name
tig0031598 gene 9569 10201 9569 ID=Msa1447110;Name=Msa1447110
tig0031598 mRNA 9569 10201 9569 ID=Msa1447110-mRNA-1;Parent=Msa1447110;Name=Msa1447110-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|210
tig0031598 exon 9569 10201 9569 ID=Msa1447110-mRNA-1:exon:15748;Parent=Msa1447110-mRNA-1
tig0031598 CDS 9569 10201 9569 ID=Msa1447110-mRNA-1:cds;Parent=Msa1447110-mRNA-1
Gene Sequence

>Msa1447110

ATGATCAACAACAAAGTAACCATTCTTTTAGTTCTTATGTGTTTCATCATTGCTGCTTTTTGTTCTGATCCGGATCCTGTTATGGATTACTGCATAGCCAAATCAGAAGATAACAAATTCAGTTGCAAAAACTCATCCACAGCAACAGTTGAAGATTTCACTTTTTCTGGTATAAAACTACCAGGAAACTTCAAAGATACTGGATTTTCTTCAATGGGAGTGAATTCAAATGTGTTTCCAGGTTTAAACACTCTTGGAGTTTCATTTGTTAGAGCAGATTTTGATGTTGGTGGTGTTAATGTTCCTCACTTTCATCCAAGAGCAACAGAAGTTGCTATTGTGTTGGAGGGAAAAATTTATTCAGGTTTTGTTGATACTAAGAACAAGATTTTTGCTAAAGTTTTGGAGAAAGGTGAGGTTATGGTTTTTCCAAGAGGACTTGTGCATTTTCAGATGAATGTTGGTGATGTTCCTGCTACTATTTTTGGTAGTTTTGATAGTCAAAATCCTGGTTTGATGAAAATTCCAAATGTTGTTTTTGGATCTCAGATTAAAGATGAGCTTTTGGAAAAAGCTTTTGGACTTAATTCTAAGGAGCTTTCTAAGTTAAAGAAGAGGTTTACTCCTAGCTAA

Protein sequence

>Msa1447110

MINNKVTILLVLMCFIIAAFCSDPDPVMDYCIAKSEDNKFSCKNSSTATVEDFTFSGIKLPGNFKDTGFSSMGVNSNVFPGLNTLGVSFVRADFDVGGVNVPHFHPRATEVAIVLEGKIYSGFVDTKNKIFAKVLEKGEVMVFPRGLVHFQMNVGDVPATIFGSFDSQNPGLMKIPNVVFGSQIKDELLEKAFGLNSKELSKLKKRFTPS*