AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1218320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1218320 MtrunA17_Chr4g0068011 98.454 647 10 0 1 647 2 648 0.0 1313
Msa1218320 MtrunA17_Chr2g0294901 68.585 643 191 6 6 641 8 646 0.0 882
Msa1218320 MtrunA17_Chr2g0319581 51.535 619 289 8 30 647 53 661 0.0 609
Msa1218320 MtrunA17_Chr5g0394101 57.235 463 188 6 181 641 4 458 0.0 556
Msa1218320 MtrunA17_Chr7g0219921 26.115 471 289 19 188 645 18 442 2.60e-42 159
Msa1218320 MtrunA17_Chr2g0333731 26.452 465 265 21 203 645 35 444 4.59e-40 152
Msa1218320 MtrunA17_Chr6g0455401 26.964 471 284 21 188 645 19 442 1.19e-38 148
Msa1218320 MtrunA17_Chr2g0308371 27.897 466 281 20 192 646 19 440 3.85e-35 138
Msa1218320 MtrunA17_Chr2g0308381 25.107 466 294 18 192 647 29 449 1.47e-34 137
Msa1218320 MtrunA17_Chr6g0455411 26.984 441 253 23 203 627 26 413 5.82e-34 134
Msa1218320 MtrunA17_Chr5g0394091 43.662 142 80 0 28 169 36 177 3.83e-33 127
Msa1218320 MtrunA17_Chr3g0145441 24.057 424 269 15 203 617 49 428 5.41e-33 132
Msa1218320 MtrunA17_Chr6g0455391 25.714 420 257 17 203 617 34 403 3.21e-32 129
Msa1218320 MtrunA17_Chr7g0219931 26.230 427 251 21 203 618 26 399 6.01e-31 125
Msa1218320 MtrunA17_Chr4g0067991 52.273 88 40 2 547 634 7 92 3.04e-17 78.6
Msa1218320 MtrunA17_Chr4g0067991 85.294 34 5 0 164 197 14 47 3.68e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1218320 AT4G16120.1 67.662 603 190 4 31 631 46 645 0.0 860
Msa1218320 AT3G16860.1 64.640 625 207 7 27 646 36 651 0.0 820
Msa1218320 AT5G49270.1 60.806 620 237 5 31 647 45 661 0.0 796
Msa1218320 AT3G20580.1 52.327 623 285 5 27 642 54 671 0.0 658
Msa1218320 AT4G27110.1 51.057 615 293 5 31 645 48 654 0.0 658
Msa1218320 AT5G60920.1 26.531 441 268 19 204 632 44 440 1.11e-36 143
Msa1218320 AT3G29810.1 26.477 457 271 21 204 646 36 441 8.76e-36 140
Msa1218320 AT3G02210.1 26.539 471 284 23 190 646 26 448 6.32e-35 138
Msa1218320 AT5G60920.2 26.316 437 266 19 208 632 1 393 6.43e-35 137
Msa1218320 AT5G15630.1 26.503 449 268 21 203 638 27 426 1.61e-34 136
Msa1218320 AT1G09790.1 26.932 427 246 17 204 618 43 415 8.81e-34 135
Msa1218320 AT1G09790.2 26.932 427 246 17 204 618 32 404 9.57e-34 134
Msa1218320 AT5G15630.3 26.376 436 258 21 220 643 8 392 1.19e-31 127
Msa1218320 AT5G15630.4 26.353 425 247 21 229 643 11 379 1.12e-30 124
Msa1218320 AT5G15630.2 26.353 425 247 21 229 643 11 379 1.12e-30 124
Msa1218320 AT5G15630.5 26.687 326 196 16 203 522 27 315 1.23e-24 106

Find 199 sgRNAs with CRISPR-Local

Find 252 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCGGTAGGGTTGCTTCTT+TGG 0.158531 8_1:+80931736 None:intergenic
TTACTAGGATCCATAGATTT+TGG 0.187680 8_1:+80930245 None:intergenic
TTCTTTCAACCTTGTTATTA+TGG 0.200360 8_1:-80929069 Msa1218320:three_prime_UTR
ACATGTTCTTGCTGTATTCT+TGG 0.221335 8_1:+80929908 None:intergenic
ATTAACACCACCTATTTCTA+TGG 0.228436 8_1:+80929581 None:intergenic
CAAGCCTTCCGAGAGGATTT+TGG 0.236120 8_1:+80930516 None:intergenic
CATGTTCTTGCTGTATTCTT+GGG 0.256699 8_1:+80929909 None:intergenic
CCCATTGAAGAAGACTTTCT+TGG 0.260858 8_1:+80929389 None:intergenic
CTCTCGGAAGGCTTGATAAT+TGG 0.261046 8_1:-80930508 Msa1218320:CDS
TCATCGCTGTTGTGTCTCTT+TGG 0.271570 8_1:+80931911 None:intergenic
CATCGCTGTTGTGTCTCTTT+GGG 0.272122 8_1:+80931912 None:intergenic
CCGTCTGTTCTGGATGCTTC+TGG 0.272420 8_1:-80930428 Msa1218320:CDS
GATCCTATGATTCAAATTAC+TGG 0.274911 8_1:-80930559 Msa1218320:CDS
TACTATATTGCCAAAATCTA+TGG 0.276132 8_1:-80930255 Msa1218320:CDS
ATCGGTAGGGTTGCTTCTTT+GGG 0.305055 8_1:+80931737 None:intergenic
GGAAAATTCCTTCTTGTTGC+CGG 0.319950 8_1:-80930283 Msa1218320:CDS
TTACTGTCAGATTATAATAA+AGG 0.326170 8_1:-80929726 Msa1218320:CDS
TTCCAACCAGTGGTGCTTCC+AGG 0.345344 8_1:-80929341 Msa1218320:CDS
CAGGCTCAGGTCAGTCTTGT+TGG 0.349029 8_1:-80931490 Msa1218320:CDS
AGGAGCTTATCCGTCTGTTC+TGG 0.349215 8_1:-80930438 Msa1218320:CDS
AAGTTTCTGCCGCGTCAAAA+TGG 0.352120 8_1:-80930611 Msa1218320:CDS
GTAATGTTTGCTTTGAACTT+AGG 0.358603 8_1:+80930644 None:intergenic
CCCAAGAAAGTCTTCTTCAA+TGG 0.363886 8_1:-80929390 Msa1218320:CDS
GGTTAAACGCGTTCAAGAAA+TGG 0.369116 8_1:+80932054 None:intergenic
TACGATAATAATGCAAGGTT+TGG 0.371365 8_1:-80929556 Msa1218320:CDS
CCAAGAAAGTCTTCTTCAAT+GGG 0.375765 8_1:-80929389 Msa1218320:CDS
AACGGCAACCCTGTTGGATC+TGG 0.382141 8_1:+80930071 None:intergenic
TCACCGCAAGGTGGTGGGTT+TGG 0.383667 8_1:+80929777 None:intergenic
CCATTGAAGAAGACTTTCTT+GGG 0.383850 8_1:+80929390 None:intergenic
ACCGTTGTTGAGCACGCTTA+AGG 0.403977 8_1:+80931705 None:intergenic
TTGATAATTGGAGGTTGAGC+TGG 0.407068 8_1:-80930496 Msa1218320:CDS
TCAGTATCATTGAATTTAGT+AGG 0.408040 8_1:+80930311 None:intergenic
AATGCAAGCGCCATAGAAAT+AGG 0.411365 8_1:-80929591 Msa1218320:CDS
GGAGGACCGCATTTGTAGTC+AGG 0.413192 8_1:+80930119 None:intergenic
TTCCTTCCTGGAAGCACCAC+TGG 0.414288 8_1:+80929335 None:intergenic
AGCTTTCACCACCACAGAAC+TGG 0.416049 8_1:-80930163 Msa1218320:CDS
CGTACAGATCCTAGGGTGCC+TGG 0.420757 8_1:-80929489 Msa1218320:CDS
GCTTCAAATTTCGAATTTGA+AGG 0.422056 8_1:-80931943 Msa1218320:five_prime_UTR
CGCTTAAGGTTGATTCAAAT+CGG 0.422598 8_1:+80931719 None:intergenic
CTGGTTTACTGCTGTGCAAA+TGG 0.424089 8_1:-80929652 Msa1218320:CDS
GCCAAGGCTTATGCGTGGGC+GGG 0.430061 8_1:-80929819 Msa1218320:CDS
AGTAGGAGGAAGATCAATAA+TGG 0.431671 8_1:+80930328 None:intergenic
TGCCCAGTAATTTGAATCAT+AGG 0.431744 8_1:+80930556 None:intergenic
TTGGCAATATAGTACCGTTC+CGG 0.432627 8_1:+80930264 None:intergenic
AGCAAAGGAAAATTCCTTCC+TGG 0.437794 8_1:+80929323 None:intergenic
GCATTTACTTGATGTTCCCT+TGG 0.438776 8_1:+80929998 None:intergenic
GAGGGATTGAACTACCTTGT+TGG 0.439092 8_1:-80929534 Msa1218320:CDS
GTCTTCGGCAACGGAACATC+AGG 0.440318 8_1:+80931445 None:intergenic
AGGCTCAGGTCAGTCTTGTT+GGG 0.441362 8_1:-80931489 Msa1218320:CDS
TTAGCAAGCTTGATCGTCTT+CGG 0.442830 8_1:+80931430 None:intergenic
TGTTGTTTCCCAGGCACCCT+AGG 0.445041 8_1:+80929480 None:intergenic
CTAACCCTCGTACAGATCCT+AGG 0.448064 8_1:-80929497 Msa1218320:CDS
ACGCATAAGCCTTGGCAGTT+CGG 0.454629 8_1:+80929826 None:intergenic
TCCTGAACCGCAAAATCAAA+TGG 0.454968 8_1:+80929242 None:intergenic
CCACGAACAACACTGATTCC+AGG 0.455604 8_1:+80929426 None:intergenic
CCAAGGCTTATGCGTGGGCG+GGG 0.459506 8_1:-80929818 Msa1218320:CDS
TTGTTACTGTTAGGTAAGAA+TGG 0.460415 8_1:-80930692 Msa1218320:intron
GATAATAATGCAAGGTTTGG+AGG 0.462661 8_1:-80929553 Msa1218320:CDS
CTGTGGAAAACCAGCGAAAA+CGG 0.467332 8_1:+80931561 None:intergenic
CAAGGTGGTGGGTTTGGCGT+TGG 0.470344 8_1:+80929783 None:intergenic
CAGTTATCACCGCAAGGTGG+TGG 0.470714 8_1:+80929771 None:intergenic
ACTGAGAATCCAGATCCAAC+AGG 0.471504 8_1:-80930080 Msa1218320:CDS
TTGGCAGTTCGGTTCTCAAA+AGG 0.472608 8_1:+80929837 None:intergenic
TCGGAAGGCTTGATAATTGG+AGG 0.474793 8_1:-80930505 Msa1218320:CDS
TACCTTGTTGGAGAAACAAA+TGG 0.475054 8_1:-80929522 Msa1218320:CDS
TTCTTTGGGTCGGTGACGTT+AGG 0.475843 8_1:+80931751 None:intergenic
ACGATCAAGCTTGCTAATGA+TGG 0.476555 8_1:-80931424 Msa1218320:CDS
ACTGCGGTGTGAGCATCAAC+TGG 0.476725 8_1:-80929752 Msa1218320:CDS
TTCACAAAGAAAACTACTCC+TGG 0.480154 8_1:-80929444 Msa1218320:CDS
AAACGGTGCCGTTTCCAACT+AGG 0.480916 8_1:+80931578 None:intergenic
AACTGTAATGGCAAGTTGGC+AGG 0.483520 8_1:-80930042 Msa1218320:CDS
ATTACTGACTGTTGTTTCCC+AGG 0.485126 8_1:+80929471 None:intergenic
GTGTTGTTCGTGGTGGTGAC+GGG 0.485449 8_1:-80929416 Msa1218320:CDS
TGTTACTGTTAGGTAAGAAT+GGG 0.485556 8_1:-80930691 Msa1218320:intron
CCCCGCCCACGCATAAGCCT+TGG 0.486167 8_1:+80929818 None:intergenic
TCAGTAGGACTAACACGAAC+CGG 0.494414 8_1:+80930098 None:intergenic
TTAGTATGATTGTTGCTAAG+AGG 0.496024 8_1:+80931854 None:intergenic
TCATTCTTGTTTCTGTGACA+AGG 0.496740 8_1:+80929181 None:intergenic
GTAGGGTTGCTTCTTTGGGT+CGG 0.497835 8_1:+80931741 None:intergenic
AACGGTGCCGTTTCCAACTA+GGG 0.498754 8_1:+80931579 None:intergenic
ATTGGAGGTTGAGCTGGGAC+TGG 0.498839 8_1:-80930490 Msa1218320:CDS
CCACAGAACTGGAAGATTAG+TGG 0.499210 8_1:-80930152 Msa1218320:CDS
AGTGTTGTTCGTGGTGGTGA+CGG 0.500030 8_1:-80929417 Msa1218320:CDS
TGTCAGATTATAATAAAGGC+TGG 0.507071 8_1:-80929722 Msa1218320:CDS
AATTCAATGATACTGACGTT+GGG 0.508579 8_1:-80930304 Msa1218320:CDS
GTGCCGTTTCCAACTAGGGC+AGG 0.511181 8_1:+80931583 None:intergenic
ATGTTCTTGCTGTATTCTTG+GGG 0.511347 8_1:+80929910 None:intergenic
ATAATAAAGGCTGGAGTGCA+AGG 0.512659 8_1:-80929713 Msa1218320:CDS
GTATGAATCAAATTCTATCC+AGG 0.513520 8_1:+80929209 None:intergenic
ACCGTCAGCGAGAACAGCAT+TGG 0.514805 8_1:+80931615 None:intergenic
GCCGTTAAACAAAACTGTAA+TGG 0.515209 8_1:-80930054 Msa1218320:CDS
TCCAATGCTGTTCTCGCTGA+CGG 0.515524 8_1:-80931616 Msa1218320:CDS
CTCCATTTGTTTCTCCAACA+AGG 0.515735 8_1:+80929520 None:intergenic
TCGTCTTCTTGTTATAGTAG+TGG 0.516006 8_1:-80929272 Msa1218320:exon
AATCTCGAAATAGACTTACT+AGG 0.517229 8_1:+80930230 None:intergenic
TGATAATTGGAGGTTGAGCT+GGG 0.519457 8_1:-80930495 Msa1218320:CDS
TGACTACAAATGCGGTCCTC+CGG 0.521089 8_1:-80930117 Msa1218320:CDS
TGCCAAGGCTTATGCGTGGG+CGG 0.522498 8_1:-80929820 Msa1218320:CDS
ACTACCCTCCCTGCCCTAGT+TGG 0.525815 8_1:-80931592 Msa1218320:CDS
TTCCTGGAAGCACCACTGGT+TGG 0.528051 8_1:+80929339 None:intergenic
ATTCCTTCTTGTTGCCGGAA+CGG 0.530325 8_1:-80930278 Msa1218320:CDS
CAAATGGACAAAGCAGCTGC+AGG 0.530745 8_1:-80929636 Msa1218320:CDS
GTTGGATCTGGATTCTCAGT+AGG 0.538813 8_1:+80930083 None:intergenic
TTTGACTCAGATGCAGGCTC+AGG 0.539352 8_1:-80931503 Msa1218320:CDS
ACAAAACTGTAATGGCAAGT+TGG 0.539967 8_1:-80930046 Msa1218320:CDS
CTCCCTGCCCTAGTTGGAAA+CGG 0.540667 8_1:-80931586 Msa1218320:CDS
AGCTCGGATCGGTTAAGCAC+TGG 0.541846 8_1:+80930182 None:intergenic
TCGGAGGGAAGCCACATGGC+AGG 0.542326 8_1:+80929873 None:intergenic
AGCTTGATCGTCTTCGGCAA+CGG 0.542358 8_1:+80931436 None:intergenic
AACTGCCAAGGCTTATGCGT+GGG 0.543903 8_1:-80929823 Msa1218320:CDS
TGATTCAAATTACTGGGCAG+AGG 0.546438 8_1:-80930552 Msa1218320:CDS
GGGTGATTTGACTCAGATGC+AGG 0.546490 8_1:-80931509 Msa1218320:CDS
ATCCTATGATTCAAATTACT+GGG 0.547534 8_1:-80930558 Msa1218320:CDS
TTGTTTAACGGCAACCCTGT+TGG 0.548344 8_1:+80930065 None:intergenic
TAAGGTTGATTCAAATCGGT+AGG 0.548344 8_1:+80931723 None:intergenic
GTACAGATCCTAGGGTGCCT+GGG 0.550393 8_1:-80929488 Msa1218320:CDS
GGAAAAGAAGGTACCTTGAC+CGG 0.550526 8_1:+80931381 None:intergenic
GTTCTGTGGTGGTGAAAGCT+CGG 0.556715 8_1:+80930166 None:intergenic
ATAATAATGCAAGGTTTGGA+GGG 0.559550 8_1:-80929552 Msa1218320:CDS
CAGTTGAACTCCGACGGAGG+AGG 0.559901 8_1:+80931777 None:intergenic
GTCTCGATGAACTCAAGTCA+TGG 0.560708 8_1:-80931684 Msa1218320:CDS
AACGCGTTCAAGAAATGGAA+AGG 0.561656 8_1:+80932059 None:intergenic
CCTGGAATCAGTGTTGTTCG+TGG 0.565292 8_1:-80929426 Msa1218320:CDS
AGGAACAAGGACAGCATCGG+AGG 0.565981 8_1:+80929857 None:intergenic
GAGTTCATATTTACAATGAA+AGG 0.567142 8_1:-80930458 Msa1218320:CDS
GTGTAACATTACAGCAGCCA+AGG 0.568058 8_1:-80930015 Msa1218320:CDS
TATTTCGAGATTCCAAATGC+AGG 0.568240 8_1:-80930219 Msa1218320:CDS
AAGACTGCTGTTGAAACGGC+GGG 0.569772 8_1:-80931529 Msa1218320:CDS
TCTGAAGACTGCTGTTGAAA+CGG 0.571130 8_1:-80931533 Msa1218320:CDS
AGTTATCACCGCAAGGTGGT+GGG 0.573185 8_1:+80929772 None:intergenic
AAGGTTGATTCAAATCGGTA+GGG 0.577202 8_1:+80931724 None:intergenic
TATATTTCGTTTCTCTCACA+CGG 0.579508 8_1:-80931965 Msa1218320:five_prime_UTR
TTTGGGTCGGTGACGTTAGG+CGG 0.582203 8_1:+80931754 None:intergenic
CCACTGGTTGGAAGTACAGA+AGG 0.582721 8_1:+80929351 None:intergenic
CTAATGTATTGAATTGTCAG+AGG 0.584525 8_1:-80930358 Msa1218320:CDS
TCTAATCTAGACATAAAGCA+TGG 0.584608 8_1:+80929123 None:intergenic
TGCCGTTTCCAACTAGGGCA+GGG 0.587865 8_1:+80931584 None:intergenic
AAAAGGAACAAGGACAGCAT+CGG 0.590596 8_1:+80929854 None:intergenic
ATCGGTAACCAAAATCCTCT+CGG 0.591098 8_1:-80930524 Msa1218320:CDS
AAATTCAATGATACTGACGT+TGG 0.593430 8_1:-80930305 Msa1218320:CDS
ACGCGTTCAAGAAATGGAAA+GGG 0.594937 8_1:+80932060 None:intergenic
TAGAAGCATCAAAGTAGCAA+AGG 0.598275 8_1:+80929308 None:intergenic
TTCGGCAACGGAACATCAGG+CGG 0.599231 8_1:+80931448 None:intergenic
GTTTCCAACTAGGGCAGGGA+GGG 0.601267 8_1:+80931588 None:intergenic
TAACCCTCGTACAGATCCTA+GGG 0.603078 8_1:-80929496 Msa1218320:CDS
GTCTCATACTCCTCCTCCGT+CGG 0.603483 8_1:-80931787 Msa1218320:CDS
CCTTCTGTACTTCCAACCAG+TGG 0.607323 8_1:-80929351 Msa1218320:CDS
AGCATCGGAGGGAAGCCACA+TGG 0.607549 8_1:+80929869 None:intergenic
CCAGAAGCATCCAGAACAGA+CGG 0.608061 8_1:+80930428 None:intergenic
GGAGGAAGATCAATAATGGT+AGG 0.609021 8_1:+80930332 None:intergenic
GTAACCAAAATCCTCTCGGA+AGG 0.609632 8_1:-80930520 Msa1218320:CDS
ACCTTAAGCGTGCTCAACAA+CGG 0.611367 8_1:-80931706 Msa1218320:CDS
CCACTAATCTTCCAGTTCTG+TGG 0.611751 8_1:+80930152 None:intergenic
AATAATACGATAATAATGCA+AGG 0.613339 8_1:-80929561 Msa1218320:CDS
AACCAGTGGTGCTTCCAGGA+AGG 0.613395 8_1:-80929337 Msa1218320:CDS
GAAAGTCTTCTTCAATGGGG+AGG 0.614351 8_1:-80929385 Msa1218320:CDS
GAGGACCGCATTTGTAGTCA+GGG 0.616412 8_1:+80930120 None:intergenic
ATCGTTACACGATTGTGCAT+CGG 0.620456 8_1:+80931816 None:intergenic
TACTGGGCAGAGGTTACGAT+CGG 0.620728 8_1:-80930542 Msa1218320:CDS
AGGCGGCAGTTGAACTCCGA+CGG 0.621931 8_1:+80931771 None:intergenic
GTACCGTTCCGGCAACAAGA+AGG 0.625866 8_1:+80930275 None:intergenic
GGAACAAGGACAGCATCGGA+GGG 0.627005 8_1:+80929858 None:intergenic
CGGCAGTTGAACTCCGACGG+AGG 0.628137 8_1:+80931774 None:intergenic
CTCGTCGTGCTGAAACTCAA+CGG 0.629703 8_1:+80931654 None:intergenic
GGACGTTGAAGAAGCTACAA+TGG 0.631853 8_1:+80931875 None:intergenic
TCGAGGACTGAGTCGCGGTG+TGG 0.632795 8_1:-80929152 Msa1218320:three_prime_UTR
CTAATCTTCCAGTTCTGTGG+TGG 0.635482 8_1:+80930155 None:intergenic
TAATAAAGGCTGGAGTGCAA+GGG 0.636466 8_1:-80929712 Msa1218320:CDS
AACAAGAATGAGAAATATCG+AGG 0.636951 8_1:-80929169 Msa1218320:three_prime_UTR
AAGAAGACGACTAGCAACAA+TGG 0.639469 8_1:+80929285 None:intergenic
GTGGTGGTGAAAGCTCGGAT+CGG 0.642389 8_1:+80930171 None:intergenic
GTATCATTGAATTTAGTAGG+AGG 0.642696 8_1:+80930314 None:intergenic
CTGAGAATCCAGATCCAACA+GGG 0.643968 8_1:-80930079 Msa1218320:CDS
TTCGGTTCTCAAAAGGAACA+AGG 0.646440 8_1:+80929844 None:intergenic
TCGGATCGGTTAAGCACTGG+CGG 0.651916 8_1:+80930185 None:intergenic
CCACCTTGCGGTGATAACTG+CGG 0.653782 8_1:-80929768 Msa1218320:CDS
CGTTTCCAACTAGGGCAGGG+AGG 0.655032 8_1:+80931587 None:intergenic
CCTTGCAAAACGTGTGCATG+TGG 0.655324 8_1:-80929936 Msa1218320:CDS
ACGCCAAACCCACCACCTTG+CGG 0.656219 8_1:-80929780 Msa1218320:CDS
TGTAACATTACAGCAGCCAA+GGG 0.657044 8_1:-80930014 Msa1218320:CDS
ATGTATTGAATTGTCAGAGG+AGG 0.658273 8_1:-80930355 Msa1218320:CDS
GGCACCCTAGGATCTGTACG+AGG 0.663910 8_1:+80929492 None:intergenic
GCAGTCTTCAGATCCGTCTG+TGG 0.665018 8_1:+80931544 None:intergenic
GAACTGCCAAGGCTTATGCG+TGG 0.665041 8_1:-80929824 Msa1218320:CDS
GAAGACTGCTGTTGAAACGG+CGG 0.671765 8_1:-80931530 Msa1218320:CDS
CTTAACCCTGACTACAAATG+CGG 0.671840 8_1:-80930125 Msa1218320:CDS
GTACTACTGCACCTGCCATG+TGG 0.672557 8_1:-80929884 Msa1218320:CDS
AAAGAAGGTACCTTGACCGG+TGG 0.672787 8_1:+80931384 None:intergenic
ACACCGCAGTTATCACCGCA+AGG 0.673011 8_1:+80929765 None:intergenic
GGAATCAGTGTTGTTCGTGG+TGG 0.683056 8_1:-80929423 Msa1218320:CDS
TAAAAGGAAAATGAATAACA+CGG 0.684492 8_1:+80932033 None:intergenic
TCTTCTGTACCATAATAACA+AGG 0.688785 8_1:+80929060 None:intergenic
GCAAGCGCCATAGAAATAGG+TGG 0.697809 8_1:-80929588 Msa1218320:CDS
GTAGGACTAACACGAACCGG+AGG 0.701832 8_1:+80930101 None:intergenic
CAAGAAAGTCTTCTTCAATG+GGG 0.710859 8_1:-80929388 Msa1218320:CDS
AAATATCGAGGACTGAGTCG+CGG 0.712727 8_1:-80929157 Msa1218320:three_prime_UTR
CAATTATCAAGCCTTCCGAG+AGG 0.713612 8_1:+80930509 None:intergenic
GCACCCTAGGATCTGTACGA+GGG 0.745402 8_1:+80929493 None:intergenic
CCGCAGTTATCACCGCAAGG+TGG 0.768131 8_1:+80929768 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTTTAATTAAAGATAC+TGG + chr8_1:80929065-80929084 None:intergenic 10.0%
!!! ATTTAACCTTTTTTTTTAAA+TGG - chr8_1:80930348-80930367 Msa1218320:CDS 10.0%
!!! AGATAAAATTGTTTTTTCTT+AGG + chr8_1:80930235-80930254 None:intergenic 15.0%
!! TAACAACCATTTAAAAAAAA+AGG + chr8_1:80930357-80930376 None:intergenic 15.0%
!! TAAAAGGAAAATGAATAACA+CGG + chr8_1:80929033-80929052 None:intergenic 20.0%
!!! ATACTGGTTAATTTTGTAAA+AGG + chr8_1:80929049-80929068 None:intergenic 20.0%
!!! AAAGCAAAAAATCTAAAACA+AGG + chr8_1:80929706-80929725 None:intergenic 20.0%
!! TTGAATTTATATGTATCGTT+TGG - chr8_1:80929896-80929915 Msa1218320:CDS 20.0%
!!! TTTTTCCAATTAATGTTACA+AGG - chr8_1:80929979-80929998 Msa1218320:CDS 20.0%
!!! TTTTCCAATTAATGTTACAA+GGG - chr8_1:80929980-80929999 Msa1218320:CDS 20.0%
!! ACAACTTAATTAAAAGCTTA+AGG + chr8_1:80930309-80930328 None:intergenic 20.0%
!!! ATTAAGTTGTTTATTGAAAC+AGG - chr8_1:80930319-80930338 Msa1218320:CDS 20.0%
!! TTACTGTCAGATTATAATAA+AGG - chr8_1:80931337-80931356 Msa1218320:intron 20.0%
!! AATAATACGATAATAATGCA+AGG - chr8_1:80931502-80931521 Msa1218320:CDS 20.0%
!!! GAAATATGTTACTTTTCTAT+TGG - chr8_1:80932045-80932064 Msa1218320:five_prime_UTR 20.0%
!! AATCTAAAACAAGGAAAAGA+AGG + chr8_1:80929697-80929716 None:intergenic 25.0%
!!! TAGATTTTTTGCTTTTGTCT+AGG - chr8_1:80929711-80929730 Msa1218320:CDS 25.0%
!! TCTAAGATTTGTTTCAAATC+AGG - chr8_1:80929749-80929768 Msa1218320:CDS 25.0%
!!! AGGGTTTTGTAATTTGAATA+AGG - chr8_1:80929779-80929798 Msa1218320:CDS 25.0%
!! AATTCAATGATCTAGCTTAT+GGG + chr8_1:80929883-80929902 None:intergenic 25.0%
!! AAATTCAATGATCTAGCTTA+TGG + chr8_1:80929884-80929903 None:intergenic 25.0%
! AATACCCTTGTAACATTAAT+TGG + chr8_1:80929987-80930006 None:intergenic 25.0%
! TTGACTAAAAAAGAACCATT+TGG + chr8_1:80930075-80930094 None:intergenic 25.0%
! CAATTATCAAAAGTTCTCTA+GGG + chr8_1:80930212-80930231 None:intergenic 25.0%
! TTTAAATGGTTGTTACTGTT+AGG - chr8_1:80930362-80930381 Msa1218320:CDS 25.0%
! ATCCTATGATTCAAATTACT+GGG - chr8_1:80930505-80930524 Msa1218320:CDS 25.0%
! GAGTTCATATTTACAATGAA+AGG - chr8_1:80930605-80930624 Msa1218320:CDS 25.0%
! TCAGTATCATTGAATTTAGT+AGG + chr8_1:80930755-80930774 None:intergenic 25.0%
! TACTATATTGCCAAAATCTA+TGG - chr8_1:80930808-80930827 Msa1218320:intron 25.0%
!!! TTATGTTCCATTTGATTTTG+CGG - chr8_1:80931814-80931833 Msa1218320:CDS 25.0%
!!! TTCTTTCAACCTTGTTATTA+TGG - chr8_1:80931994-80932013 Msa1218320:five_prime_UTR 25.0%
TATATTTCGTTTCTCTCACA+CGG - chr8_1:80929098-80929117 Msa1218320:three_prime_UTR 30.0%
GCTTCAAATTTCGAATTTGA+AGG - chr8_1:80929120-80929139 Msa1218320:three_prime_UTR 30.0%
TTAGTATGATTGTTGCTAAG+AGG + chr8_1:80929212-80929231 None:intergenic 30.0%
TTTCAAATCAGGTGTGATAA+GGG - chr8_1:80929760-80929779 Msa1218320:CDS 30.0%
AATTTGAATAAGGCTCTGTT+TGG - chr8_1:80929789-80929808 Msa1218320:CDS 30.0%
CGTTTGGTAAAATTCATTGT+TGG - chr8_1:80929912-80929931 Msa1218320:CDS 30.0%
TGTCTATAAGCTCAAATATG+TGG - chr8_1:80930118-80930137 Msa1218320:CDS 30.0%
GCAATTATCAAAAGTTCTCT+AGG + chr8_1:80930213-80930232 None:intergenic 30.0%
TTGTTACTGTTAGGTAAGAA+TGG - chr8_1:80930371-80930390 Msa1218320:CDS 30.0%
TGTTACTGTTAGGTAAGAAT+GGG - chr8_1:80930372-80930391 Msa1218320:CDS 30.0%
! GTAATGTTTGCTTTGAACTT+AGG + chr8_1:80930422-80930441 None:intergenic 30.0%
GATCCTATGATTCAAATTAC+TGG - chr8_1:80930504-80930523 Msa1218320:CDS 30.0%
CTAATGTATTGAATTGTCAG+AGG - chr8_1:80930705-80930724 Msa1218320:intron 30.0%
GTATCATTGAATTTAGTAGG+AGG + chr8_1:80930752-80930771 None:intergenic 30.0%
AAATTCAATGATACTGACGT+TGG - chr8_1:80930758-80930777 Msa1218320:intron 30.0%
AATTCAATGATACTGACGTT+GGG - chr8_1:80930759-80930778 Msa1218320:intron 30.0%
! TTACTAGGATCCATAGATTT+TGG + chr8_1:80930821-80930840 None:intergenic 30.0%
AATCTCGAAATAGACTTACT+AGG + chr8_1:80930836-80930855 None:intergenic 30.0%
!!! GCCATTACAGTTTTGTTTAA+CGG + chr8_1:80931013-80931032 None:intergenic 30.0%
TGTCAGATTATAATAAAGGC+TGG - chr8_1:80931341-80931360 Msa1218320:intron 30.0%
!! ATTAACACCACCTATTTCTA+TGG + chr8_1:80931485-80931504 None:intergenic 30.0%
TACGATAATAATGCAAGGTT+TGG - chr8_1:80931507-80931526 Msa1218320:CDS 30.0%
ATAATAATGCAAGGTTTGGA+GGG - chr8_1:80931511-80931530 Msa1218320:CDS 30.0%
GTATGAATCAAATTCTATCC+AGG + chr8_1:80931857-80931876 None:intergenic 30.0%
AACAAGAATGAGAAATATCG+AGG - chr8_1:80931894-80931913 Msa1218320:CDS 30.0%
TCTAATCTAGACATAAAGCA+TGG + chr8_1:80931943-80931962 None:intergenic 30.0%
TCTTCTGTACCATAATAACA+AGG + chr8_1:80932006-80932025 None:intergenic 30.0%
! AAGGTTGATTCAAATCGGTA+GGG + chr8_1:80929342-80929361 None:intergenic 35.0%
! TAAGGTTGATTCAAATCGGT+AGG + chr8_1:80929343-80929362 None:intergenic 35.0%
! CGCTTAAGGTTGATTCAAAT+CGG + chr8_1:80929347-80929366 None:intergenic 35.0%
TTCATCTGTCAAAAAACCAC+CGG - chr8_1:80929663-80929682 Msa1218320:CDS 35.0%
GTTTCAAATCAGGTGTGATA+AGG - chr8_1:80929759-80929778 Msa1218320:CDS 35.0%
ATAAGCTCGTAAGAAAGTGA+TGG - chr8_1:80930046-80930065 Msa1218320:CDS 35.0%
AGAAAGTGATGGTTACCAAA+TGG - chr8_1:80930057-80930076 Msa1218320:CDS 35.0%
TAAGCAGATAAACTCTCTCA+TGG + chr8_1:80930157-80930176 None:intergenic 35.0%
TGCCCAGTAATTTGAATCAT+AGG + chr8_1:80930510-80930529 None:intergenic 35.0%
!! GATGCTTCTGGTTGTATTTT+TGG - chr8_1:80930647-80930666 Msa1218320:CDS 35.0%
!!! GGTTGTATTTTTGGTAAGCA+AGG - chr8_1:80930656-80930675 Msa1218320:CDS 35.0%
ATGTATTGAATTGTCAGAGG+AGG - chr8_1:80930708-80930727 Msa1218320:intron 35.0%
AGTAGGAGGAAGATCAATAA+TGG + chr8_1:80930738-80930757 None:intergenic 35.0%
TATTTCGAGATTCCAAATGC+AGG - chr8_1:80930844-80930863 Msa1218320:intron 35.0%
GCCGTTAAACAAAACTGTAA+TGG - chr8_1:80931009-80931028 Msa1218320:intron 35.0%
ACAAAACTGTAATGGCAAGT+TGG - chr8_1:80931017-80931036 Msa1218320:intron 35.0%
! ATGTTCTTGCTGTATTCTTG+GGG + chr8_1:80931156-80931175 None:intergenic 35.0%
! CATGTTCTTGCTGTATTCTT+GGG + chr8_1:80931157-80931176 None:intergenic 35.0%
! ACATGTTCTTGCTGTATTCT+TGG + chr8_1:80931158-80931177 None:intergenic 35.0%
! AAGGGTCACACTTTTCAATT+GGG - chr8_1:80931369-80931388 Msa1218320:intron 35.0%
GATAATAATGCAAGGTTTGG+AGG - chr8_1:80931510-80931529 Msa1218320:CDS 35.0%
TACCTTGTTGGAGAAACAAA+TGG - chr8_1:80931541-80931560 Msa1218320:CDS 35.0%
TTCACAAAGAAAACTACTCC+TGG - chr8_1:80931619-80931638 Msa1218320:CDS 35.0%
CCATTGAAGAAGACTTTCTT+GGG + chr8_1:80931676-80931695 None:intergenic 35.0%
CCAAGAAAGTCTTCTTCAAT+GGG - chr8_1:80931674-80931693 Msa1218320:CDS 35.0%
CAAGAAAGTCTTCTTCAATG+GGG - chr8_1:80931675-80931694 Msa1218320:CDS 35.0%
!! TAGAAGCATCAAAGTAGCAA+AGG + chr8_1:80931758-80931777 None:intergenic 35.0%
! TCGTCTTCTTGTTATAGTAG+TGG - chr8_1:80931791-80931810 Msa1218320:CDS 35.0%
!! TCATTCTTGTTTCTGTGACA+AGG + chr8_1:80931885-80931904 None:intergenic 35.0%
ACGCGTTCAAGAAATGGAAA+GGG + chr8_1:80929006-80929025 None:intergenic 40.0%
AACGCGTTCAAGAAATGGAA+AGG + chr8_1:80929007-80929026 None:intergenic 40.0%
GGTTAAACGCGTTCAAGAAA+TGG + chr8_1:80929012-80929031 None:intergenic 40.0%
ATCGTTACACGATTGTGCAT+CGG + chr8_1:80929250-80929269 None:intergenic 40.0%
TCTGAAGACTGCTGTTGAAA+CGG - chr8_1:80929530-80929549 Msa1218320:CDS 40.0%
!!! AGTCTTGTTGGGACTGTTTT+TGG - chr8_1:80929585-80929604 Msa1218320:CDS 40.0%
TTAGCAAGCTTGATCGTCTT+CGG + chr8_1:80929636-80929655 None:intergenic 40.0%
!! ACGATCAAGCTTGCTAATGA+TGG - chr8_1:80929639-80929658 Msa1218320:CDS 40.0%
! CTGTTTGGATCGACTTATTG+AGG - chr8_1:80929804-80929823 Msa1218320:CDS 40.0%
! CGACTTATTGAGGCTCTTTT+TGG - chr8_1:80929814-80929833 Msa1218320:CDS 40.0%
AAACTCTCTCATGGTCTGTT+TGG + chr8_1:80930148-80930167 None:intergenic 40.0%
!! GTAAGATCACCATTTTGACG+CGG + chr8_1:80930464-80930483 None:intergenic 40.0%
TGATTCAAATTACTGGGCAG+AGG - chr8_1:80930511-80930530 Msa1218320:CDS 40.0%
ATCGGTAACCAAAATCCTCT+CGG - chr8_1:80930539-80930558 Msa1218320:CDS 40.0%
! TTGATAATTGGAGGTTGAGC+TGG - chr8_1:80930567-80930586 Msa1218320:CDS 40.0%
TGATAATTGGAGGTTGAGCT+GGG - chr8_1:80930568-80930587 Msa1218320:CDS 40.0%
GGAGGAAGATCAATAATGGT+AGG + chr8_1:80930734-80930753 None:intergenic 40.0%
GGAAAATTCCTTCTTGTTGC+CGG - chr8_1:80930780-80930799 Msa1218320:intron 40.0%
TTGGCAATATAGTACCGTTC+CGG + chr8_1:80930802-80930821 None:intergenic 40.0%
! GCATTTTGTACACCTGCATT+TGG + chr8_1:80930859-80930878 None:intergenic 40.0%
CTTAACCCTGACTACAAATG+CGG - chr8_1:80930938-80930957 Msa1218320:intron 40.0%
TGTAACATTACAGCAGCCAA+GGG - chr8_1:80931049-80931068 Msa1218320:intron 40.0%
GCATTTACTTGATGTTCCCT+TGG + chr8_1:80931068-80931087 None:intergenic 40.0%
AAAAGGAACAAGGACAGCAT+CGG + chr8_1:80931212-80931231 None:intergenic 40.0%
TTCGGTTCTCAAAAGGAACA+AGG + chr8_1:80931222-80931241 None:intergenic 40.0%
ATAATAAAGGCTGGAGTGCA+AGG - chr8_1:80931350-80931369 Msa1218320:intron 40.0%
TAATAAAGGCTGGAGTGCAA+GGG - chr8_1:80931351-80931370 Msa1218320:intron 40.0%
! CAAGGGTCACACTTTTCAAT+TGG - chr8_1:80931368-80931387 Msa1218320:intron 40.0%
! AGGGTCACACTTTTCAATTG+GGG - chr8_1:80931370-80931389 Msa1218320:intron 40.0%
! GTGAGACTAATTTTGCAGAC+TGG - chr8_1:80931392-80931411 Msa1218320:intron 40.0%
AATGCAAGCGCCATAGAAAT+AGG - chr8_1:80931472-80931491 Msa1218320:CDS 40.0%
! CTCCATTTGTTTCTCCAACA+AGG + chr8_1:80931546-80931565 None:intergenic 40.0%
!! ATTACTGACTGTTGTTTCCC+AGG + chr8_1:80931595-80931614 None:intergenic 40.0%
CCCAAGAAAGTCTTCTTCAA+TGG - chr8_1:80931673-80931692 Msa1218320:CDS 40.0%
CCCATTGAAGAAGACTTTCT+TGG + chr8_1:80931677-80931696 None:intergenic 40.0%
AGCAAAGGAAAATTCCTTCC+TGG + chr8_1:80931743-80931762 None:intergenic 40.0%
AAGAAGACGACTAGCAACAA+TGG + chr8_1:80931781-80931800 None:intergenic 40.0%
!! TCCATTTGATTTTGCGGTTC+AGG - chr8_1:80931820-80931839 Msa1218320:CDS 40.0%
TCCTGAACCGCAAAATCAAA+TGG + chr8_1:80931824-80931843 None:intergenic 40.0%
CATCGCTGTTGTGTCTCTTT+GGG + chr8_1:80929154-80929173 None:intergenic 45.0%
TCATCGCTGTTGTGTCTCTT+TGG + chr8_1:80929155-80929174 None:intergenic 45.0%
GGACGTTGAAGAAGCTACAA+TGG + chr8_1:80929191-80929210 None:intergenic 45.0%
ATCGGTAGGGTTGCTTCTTT+GGG + chr8_1:80929329-80929348 None:intergenic 45.0%
AATCGGTAGGGTTGCTTCTT+TGG + chr8_1:80929330-80929349 None:intergenic 45.0%
ACCTTAAGCGTGCTCAACAA+CGG - chr8_1:80929357-80929376 Msa1218320:CDS 45.0%
GTCTCGATGAACTCAAGTCA+TGG - chr8_1:80929379-80929398 Msa1218320:CDS 45.0%
CTGTGGAAAACCAGCGAAAA+CGG + chr8_1:80929505-80929524 None:intergenic 45.0%
!!! TTTCGCTGGTTTTCCACAGA+CGG - chr8_1:80929506-80929525 Msa1218320:CDS 45.0%
TGTCAAAAAACCACCGGTCA+AGG - chr8_1:80929669-80929688 Msa1218320:CDS 45.0%
!! GGAAAAGAAGGTACCTTGAC+CGG + chr8_1:80929685-80929704 None:intergenic 45.0%
AAGTTTCTGCCGCGTCAAAA+TGG - chr8_1:80930452-80930471 Msa1218320:CDS 45.0%
GTAACCAAAATCCTCTCGGA+AGG - chr8_1:80930543-80930562 Msa1218320:CDS 45.0%
CAATTATCAAGCCTTCCGAG+AGG + chr8_1:80930557-80930576 None:intergenic 45.0%
! CTCTCGGAAGGCTTGATAAT+TGG - chr8_1:80930555-80930574 Msa1218320:CDS 45.0%
! TCGGAAGGCTTGATAATTGG+AGG - chr8_1:80930558-80930577 Msa1218320:CDS 45.0%
ATTCCTTCTTGTTGCCGGAA+CGG - chr8_1:80930785-80930804 Msa1218320:intron 45.0%
CTAATCTTCCAGTTCTGTGG+TGG + chr8_1:80930911-80930930 None:intergenic 45.0%
CCACTAATCTTCCAGTTCTG+TGG + chr8_1:80930914-80930933 None:intergenic 45.0%
CCACAGAACTGGAAGATTAG+TGG - chr8_1:80930911-80930930 Msa1218320:intron 45.0%
TCAGTAGGACTAACACGAAC+CGG + chr8_1:80930968-80930987 None:intergenic 45.0%
GTTGGATCTGGATTCTCAGT+AGG + chr8_1:80930983-80931002 None:intergenic 45.0%
ACTGAGAATCCAGATCCAAC+AGG - chr8_1:80930983-80931002 Msa1218320:intron 45.0%
CTGAGAATCCAGATCCAACA+GGG - chr8_1:80930984-80931003 Msa1218320:intron 45.0%
TTGTTTAACGGCAACCCTGT+TGG + chr8_1:80931001-80931020 None:intergenic 45.0%
! AACTGTAATGGCAAGTTGGC+AGG - chr8_1:80931021-80931040 Msa1218320:intron 45.0%
GTGTAACATTACAGCAGCCA+AGG - chr8_1:80931048-80931067 Msa1218320:intron 45.0%
TTGGCAGTTCGGTTCTCAAA+AGG + chr8_1:80931229-80931248 None:intergenic 45.0%
!! TTTTGAGAACCGAACTGCCA+AGG - chr8_1:80931228-80931247 Msa1218320:intron 45.0%
CTGGTTTACTGCTGTGCAAA+TGG - chr8_1:80931411-80931430 Msa1218320:CDS 45.0%
GAGGGATTGAACTACCTTGT+TGG - chr8_1:80931529-80931548 Msa1218320:CDS 45.0%
TAACCCTCGTACAGATCCTA+GGG - chr8_1:80931567-80931586 Msa1218320:CDS 45.0%
GAAAGTCTTCTTCAATGGGG+AGG - chr8_1:80931678-80931697 Msa1218320:CDS 45.0%
!!! GTTCAGGATTGCTGTTTTCC+TGG - chr8_1:80931836-80931855 Msa1218320:CDS 45.0%
AAATATCGAGGACTGAGTCG+CGG - chr8_1:80931906-80931925 Msa1218320:exon 45.0%
TTCTTTGGGTCGGTGACGTT+AGG + chr8_1:80929315-80929334 None:intergenic 50.0%
GTAGGGTTGCTTCTTTGGGT+CGG + chr8_1:80929325-80929344 None:intergenic 50.0%
ACCGTTGTTGAGCACGCTTA+AGG + chr8_1:80929361-80929380 None:intergenic 50.0%
CTCGTCGTGCTGAAACTCAA+CGG + chr8_1:80929412-80929431 None:intergenic 50.0%
TCCAATGCTGTTCTCGCTGA+CGG - chr8_1:80929447-80929466 Msa1218320:CDS 50.0%
!! AACGGTGCCGTTTCCAACTA+GGG + chr8_1:80929487-80929506 None:intergenic 50.0%
!! AAACGGTGCCGTTTCCAACT+AGG + chr8_1:80929488-80929507 None:intergenic 50.0%
GAAGACTGCTGTTGAAACGG+CGG - chr8_1:80929533-80929552 Msa1218320:CDS 50.0%
AAGACTGCTGTTGAAACGGC+GGG - chr8_1:80929534-80929553 Msa1218320:CDS 50.0%
GGGTGATTTGACTCAGATGC+AGG - chr8_1:80929554-80929573 Msa1218320:CDS 50.0%
TTTGACTCAGATGCAGGCTC+AGG - chr8_1:80929560-80929579 Msa1218320:CDS 50.0%
!! AGGCTCAGGTCAGTCTTGTT+GGG - chr8_1:80929574-80929593 Msa1218320:CDS 50.0%
AGCTTGATCGTCTTCGGCAA+CGG + chr8_1:80929630-80929649 None:intergenic 50.0%
!! AAAGAAGGTACCTTGACCGG+TGG + chr8_1:80929682-80929701 None:intergenic 50.0%
TACTGGGCAGAGGTTACGAT+CGG - chr8_1:80930521-80930540 Msa1218320:CDS 50.0%
! CAAGCCTTCCGAGAGGATTT+TGG + chr8_1:80930550-80930569 None:intergenic 50.0%
AGGAGCTTATCCGTCTGTTC+TGG - chr8_1:80930625-80930644 Msa1218320:CDS 50.0%
!! CCAGAAGCATCCAGAACAGA+CGG + chr8_1:80930638-80930657 None:intergenic 50.0%
! GTTCTGTGGTGGTGAAAGCT+CGG + chr8_1:80930900-80930919 None:intergenic 50.0%
AGCTTTCACCACCACAGAAC+TGG - chr8_1:80930900-80930919 Msa1218320:intron 50.0%
GAGGACCGCATTTGTAGTCA+GGG + chr8_1:80930946-80930965 None:intergenic 50.0%
TGACTACAAATGCGGTCCTC+CGG - chr8_1:80930946-80930965 Msa1218320:intron 50.0%
! CCACATGCACACGTTTTGCA+AGG + chr8_1:80931130-80931149 None:intergenic 50.0%
CCTTGCAAAACGTGTGCATG+TGG - chr8_1:80931127-80931146 Msa1218320:intron 50.0%
ACGCATAAGCCTTGGCAGTT+CGG + chr8_1:80931240-80931259 None:intergenic 50.0%
AACTGCCAAGGCTTATGCGT+GGG - chr8_1:80931240-80931259 Msa1218320:intron 50.0%
AGTTATCACCGCAAGGTGGT+GGG + chr8_1:80931294-80931313 None:intergenic 50.0%
CAAATGGACAAAGCAGCTGC+AGG - chr8_1:80931427-80931446 Msa1218320:CDS 50.0%
GCAAGCGCCATAGAAATAGG+TGG - chr8_1:80931475-80931494 Msa1218320:CDS 50.0%
CTAACCCTCGTACAGATCCT+AGG - chr8_1:80931566-80931585 Msa1218320:CDS 50.0%
!! CCACGAACAACACTGATTCC+AGG + chr8_1:80931640-80931659 None:intergenic 50.0%
! CCTGGAATCAGTGTTGTTCG+TGG - chr8_1:80931637-80931656 Msa1218320:CDS 50.0%
! GGAATCAGTGTTGTTCGTGG+TGG - chr8_1:80931640-80931659 Msa1218320:CDS 50.0%
! AGTGTTGTTCGTGGTGGTGA+CGG - chr8_1:80931646-80931665 Msa1218320:CDS 50.0%
! CCACTGGTTGGAAGTACAGA+AGG + chr8_1:80931715-80931734 None:intergenic 50.0%
CCTTCTGTACTTCCAACCAG+TGG - chr8_1:80931712-80931731 Msa1218320:CDS 50.0%
GTCTCATACTCCTCCTCCGT+CGG - chr8_1:80929276-80929295 Msa1218320:exon 55.0%
TTTGGGTCGGTGACGTTAGG+CGG + chr8_1:80929312-80929331 None:intergenic 55.0%
ACCGTCAGCGAGAACAGCAT+TGG + chr8_1:80929451-80929470 None:intergenic 55.0%
GTTTCCAACTAGGGCAGGGA+GGG + chr8_1:80929478-80929497 None:intergenic 55.0%
CTCCCTGCCCTAGTTGGAAA+CGG - chr8_1:80929477-80929496 Msa1218320:CDS 55.0%
! TGCCGTTTCCAACTAGGGCA+GGG + chr8_1:80929482-80929501 None:intergenic 55.0%
GCAGTCTTCAGATCCGTCTG+TGG + chr8_1:80929522-80929541 None:intergenic 55.0%
! CAGGCTCAGGTCAGTCTTGT+TGG - chr8_1:80929573-80929592 Msa1218320:CDS 55.0%
TTCGGCAACGGAACATCAGG+CGG + chr8_1:80929618-80929637 None:intergenic 55.0%
GTCTTCGGCAACGGAACATC+AGG + chr8_1:80929621-80929640 None:intergenic 55.0%
ATTGGAGGTTGAGCTGGGAC+TGG - chr8_1:80930573-80930592 Msa1218320:CDS 55.0%
! CCGTCTGTTCTGGATGCTTC+TGG - chr8_1:80930635-80930654 Msa1218320:CDS 55.0%
GTACCGTTCCGGCAACAAGA+AGG + chr8_1:80930791-80930810 None:intergenic 55.0%
!! TCGGATCGGTTAAGCACTGG+CGG + chr8_1:80930881-80930900 None:intergenic 55.0%
AGCTCGGATCGGTTAAGCAC+TGG + chr8_1:80930884-80930903 None:intergenic 55.0%
! GTGGTGGTGAAAGCTCGGAT+CGG + chr8_1:80930895-80930914 None:intergenic 55.0%
GGAGGACCGCATTTGTAGTC+AGG + chr8_1:80930947-80930966 None:intergenic 55.0%
GTAGGACTAACACGAACCGG+AGG + chr8_1:80930965-80930984 None:intergenic 55.0%
AACGGCAACCCTGTTGGATC+TGG + chr8_1:80930995-80931014 None:intergenic 55.0%
GTACTACTGCACCTGCCATG+TGG - chr8_1:80931179-80931198 Msa1218320:intron 55.0%
! GGAACAAGGACAGCATCGGA+GGG + chr8_1:80931208-80931227 None:intergenic 55.0%
! AGGAACAAGGACAGCATCGG+AGG + chr8_1:80931209-80931228 None:intergenic 55.0%
GAACTGCCAAGGCTTATGCG+TGG - chr8_1:80931239-80931258 Msa1218320:intron 55.0%
CAGTTATCACCGCAAGGTGG+TGG + chr8_1:80931295-80931314 None:intergenic 55.0%
CCACCTTGCGGTGATAACTG+CGG - chr8_1:80931295-80931314 Msa1218320:intron 55.0%
ACACCGCAGTTATCACCGCA+AGG + chr8_1:80931301-80931320 None:intergenic 55.0%
ACTGCGGTGTGAGCATCAAC+TGG - chr8_1:80931311-80931330 Msa1218320:intron 55.0%
GCACCCTAGGATCTGTACGA+GGG + chr8_1:80931573-80931592 None:intergenic 55.0%
! GTACAGATCCTAGGGTGCCT+GGG - chr8_1:80931575-80931594 Msa1218320:CDS 55.0%
!! TGTTGTTTCCCAGGCACCCT+AGG + chr8_1:80931586-80931605 None:intergenic 55.0%
! GTGTTGTTCGTGGTGGTGAC+GGG - chr8_1:80931647-80931666 Msa1218320:CDS 55.0%
TTCCAACCAGTGGTGCTTCC+AGG - chr8_1:80931722-80931741 Msa1218320:CDS 55.0%
!! TTCCTGGAAGCACCACTGGT+TGG + chr8_1:80931727-80931746 None:intergenic 55.0%
AACCAGTGGTGCTTCCAGGA+AGG - chr8_1:80931726-80931745 Msa1218320:CDS 55.0%
!! TTCCTTCCTGGAAGCACCAC+TGG + chr8_1:80931731-80931750 None:intergenic 55.0%
CAGTTGAACTCCGACGGAGG+AGG + chr8_1:80929289-80929308 None:intergenic 60.0%
AGGCGGCAGTTGAACTCCGA+CGG + chr8_1:80929295-80929314 None:intergenic 60.0%
ACTACCCTCCCTGCCCTAGT+TGG - chr8_1:80929471-80929490 Msa1218320:CDS 60.0%
CGTTTCCAACTAGGGCAGGG+AGG + chr8_1:80929479-80929498 None:intergenic 60.0%
!! GTGCCGTTTCCAACTAGGGC+AGG + chr8_1:80929483-80929502 None:intergenic 60.0%
! GGAAACGGCACCGTTTTCGC+TGG - chr8_1:80929492-80929511 Msa1218320:CDS 60.0%
! AGCATCGGAGGGAAGCCACA+TGG + chr8_1:80931197-80931216 None:intergenic 60.0%
TGCCAAGGCTTATGCGTGGG+CGG - chr8_1:80931243-80931262 Msa1218320:intron 60.0%
!! CAAGGTGGTGGGTTTGGCGT+TGG + chr8_1:80931283-80931302 None:intergenic 60.0%
ACGCCAAACCCACCACCTTG+CGG - chr8_1:80931283-80931302 Msa1218320:intron 60.0%
!! TCACCGCAAGGTGGTGGGTT+TGG + chr8_1:80931289-80931308 None:intergenic 60.0%
CCGCAGTTATCACCGCAAGG+TGG + chr8_1:80931298-80931317 None:intergenic 60.0%
GGCACCCTAGGATCTGTACG+AGG + chr8_1:80931574-80931593 None:intergenic 60.0%
! CGTACAGATCCTAGGGTGCC+TGG - chr8_1:80931574-80931593 Msa1218320:CDS 60.0%
CGGCAGTTGAACTCCGACGG+AGG + chr8_1:80929292-80929311 None:intergenic 65.0%
TCGGAGGGAAGCCACATGGC+AGG + chr8_1:80931193-80931212 None:intergenic 65.0%
GCCAAGGCTTATGCGTGGGC+GGG - chr8_1:80931244-80931263 Msa1218320:intron 65.0%
CCAAGGCTTATGCGTGGGCG+GGG - chr8_1:80931245-80931264 Msa1218320:intron 65.0%
TCGAGGACTGAGTCGCGGTG+TGG - chr8_1:80931911-80931930 Msa1218320:exon 65.0%
CCCCGCCCACGCATAAGCCT+TGG + chr8_1:80931248-80931267 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 80928999 80932086 80928999 ID=Msa1218320;Name=Msa1218320
chr8_1 mRNA 80928999 80932086 80928999 ID=Msa1218320-mRNA-1;Parent=Msa1218320;Name=Msa1218320-mRNA-1;_AED=0.24;_eAED=0.24;_QI=172|1|1|1|1|1|2|279|647
chr8_1 exon 80928999 80930701 80928999 ID=Msa1218320-mRNA-1:exon:28560;Parent=Msa1218320-mRNA-1
chr8_1 exon 80931395 80932086 80931395 ID=Msa1218320-mRNA-1:exon:28559;Parent=Msa1218320-mRNA-1
chr8_1 five_prime_UTR 80931915 80932086 80931915 ID=Msa1218320-mRNA-1:five_prime_utr;Parent=Msa1218320-mRNA-1
chr8_1 CDS 80931395 80931914 80931395 ID=Msa1218320-mRNA-1:cds;Parent=Msa1218320-mRNA-1
chr8_1 CDS 80929278 80930701 80929278 ID=Msa1218320-mRNA-1:cds;Parent=Msa1218320-mRNA-1
chr8_1 three_prime_UTR 80928999 80929277 80928999 ID=Msa1218320-mRNA-1:three_prime_utr;Parent=Msa1218320-mRNA-1
Gene Sequence

>Msa1218320

ATGATTTCGCTACGAAACCATTGTAGCTTCTTCAACGTCCTCTTAGCAACAATCATACTAACAACGACGTCGTTTACCGATGCACAATCGTGTAACGATATACTCGTCTCATACTCCTCCTCCGTCGGAGTTCAACTGCCGCCTAACGTCACCGACCCAAAGAAGCAACCCTACCGATTTGAATCAACCTTAAGCGTGCTCAACAACGGTCTCGATGAACTCAAGTCATGGAAAGTCGCCGTTGAGTTTCAGCACGACGAGTATCTTGTTTCTGCTTCCAATGCTGTTCTCGCTGACGGTACTACCCTCCCTGCCCTAGTTGGAAACGGCACCGTTTTCGCTGGTTTTCCACAGACGGATCTGAAGACTGCTGTTGAAACGGCGGGTGATTTGACTCAGATGCAGGCTCAGGTCAGTCTTGTTGGGACTGTTTTTGGTGTAGCGCCGCCTGATGTTCCGTTGCCGAAGACGATCAAGCTTGCTAATGATGGATTCATCTGTCAAAAAACCACCGGTCAAGGTAAGAATGGGACTAATGTTTGTTGCACAAGAGATCCTAAGTTCAAAGCAAACATTACTACAGATGAAAAGTTTCTGCCGCGTCAAAATGGTGATCTTACGATCATGTATGATGTGATTAGATCCTATGATTCAAATTACTGGGCAGAGGTTACGATCGGTAACCAAAATCCTCTCGGAAGGCTTGATAATTGGAGGTTGAGCTGGGACTGGATGAATGACGAGTTCATATTTACAATGAAAGGAGCTTATCCGTCTGTTCTGGATGCTTCTGGTTGTATTTTTGGTAAGCAAGGTACATACTACAAAGAGCTTGATTTTGCTAATGTATTGAATTGTCAGAGGAGGCCTACCATTATTGATCTTCCTCCTACTAAATTCAATGATACTGACGTTGGGAAAATTCCTTCTTGTTGCCGGAACGGTACTATATTGCCAAAATCTATGGATCCTAGTAAGTCTATTTCGAGATTCCAAATGCAGGTGTACAAAATGCCGCCAGTGCTTAACCGATCCGAGCTTTCACCACCACAGAACTGGAAGATTAGTGGTTCACTTAACCCTGACTACAAATGCGGTCCTCCGGTTCGTGTTAGTCCTACTGAGAATCCAGATCCAACAGGGTTGCCGTTAAACAAAACTGTAATGGCAAGTTGGCAGGTTGTGTGTAACATTACAGCAGCCAAGGGAACATCAAGTAAATGCTGTGTCTCATTTTCAGCTTATTACAATGAATCAGTTATTCCTTGCAAAACGTGTGCATGTGGATGCCCCAAGAATACAGCAAGAACATGTAGTACTACTGCACCTGCCATGTGGCTTCCCTCCGATGCTGTCCTTGTTCCTTTTGAGAACCGAACTGCCAAGGCTTATGCGTGGGCGGGGATAAAACATCTACCAACGCCAAACCCACCACCTTGCGGTGATAACTGCGGTGTGAGCATCAACTGGCATTTACTGTCAGATTATAATAAAGGCTGGAGTGCAAGGGTCACACTTTTCAATTGGGGTGAGACTAATTTTGCAGACTGGTTTACTGCTGTGCAAATGGACAAAGCAGCTGCAGGTTTTGAGAAAATGTACTCGTTCAATGCAAGCGCCATAGAAATAGGTGGTGTTAATAATACGATAATAATGCAAGGTTTGGAGGGATTGAACTACCTTGTTGGAGAAACAAATGGAGCTAACCCTCGTACAGATCCTAGGGTGCCTGGGAAACAACAGTCAGTAATCTCATTCACAAAGAAAACTACTCCTGGAATCAGTGTTGTTCGTGGTGGTGACGGGTTTCCCAAGAAAGTCTTCTTCAATGGGGAGGAATGTTCTCTTCCTTCTGTACTTCCAACCAGTGGTGCTTCCAGGAAGGAATTTTCCTTTGCTACTTTGATGCTTCTACCATTGTTGCTAGTCGTCTTCTTGTTATAG
Protein sequence

>Msa1218320

MISLRNHCSFFNVLLATIILTTTSFTDAQSCNDILVSYSSSVGVQLPPNVTDPKKQPYRFESTLSVLNNGLDELKSWKVAVEFQHDEYLVSASNAVLADGTTLPALVGNGTVFAGFPQTDLKTAVETAGDLTQMQAQVSLVGTVFGVAPPDVPLPKTIKLANDGFICQKTTGQGKNGTNVCCTRDPKFKANITTDEKFLPRQNGDLTIMYDVIRSYDSNYWAEVTIGNQNPLGRLDNWRLSWDWMNDEFIFTMKGAYPSVLDASGCIFGKQGTYYKELDFANVLNCQRRPTIIDLPPTKFNDTDVGKIPSCCRNGTILPKSMDPSKSISRFQMQVYKMPPVLNRSELSPPQNWKISGSLNPDYKCGPPVRVSPTENPDPTGLPLNKTVMASWQVVCNITAAKGTSSKCCVSFSAYYNESVIPCKTCACGCPKNTARTCSTTAPAMWLPSDAVLVPFENRTAKAYAWAGIKHLPTPNPPPCGDNCGVSINWHLLSDYNKGWSARVTLFNWGETNFADWFTAVQMDKAAAGFEKMYSFNASAIEIGGVNNTIIMQGLEGLNYLVGETNGANPRTDPRVPGKQQSVISFTKKTTPGISVVRGGDGFPKKVFFNGEECSLPSVLPTSGASRKEFSFATLMLLPLLLVVFLL*