Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1345920 | XP_013470214.1 | 93.893 | 131 | 7 | 1 | 1 | 130 | 1 | 131 | 9.90e-84 | 251 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1345920 | G7ZZA3 | 93.893 | 131 | 7 | 1 | 1 | 130 | 1 | 131 | 4.73e-84 | 251 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0036140 | Msa1345920 | 0.801708 | 7.926807e-49 | -8.615850e-47 |
| Msa0114060 | Msa1345920 | 0.805648 | 1.206391e-49 | -8.615850e-47 |
| Msa1345920 | Msa1392260 | 0.810154 | 1.327578e-50 | -8.615850e-47 |
| Msa1345920 | Msa1415910 | 0.800098 | 1.690455e-48 | -8.615850e-47 |
| Msa1345920 | Msa1420290 | 0.808203 | 3.475545e-50 | -8.615850e-47 |
| Msa1345920 | Msa1451570 | 0.805297 | 1.428967e-49 | -8.615850e-47 |
| Msa0607540 | Msa1345920 | 0.804114 | 2.523797e-49 | -8.615850e-47 |
| Msa0657970 | Msa1345920 | 0.801888 | 7.279045e-49 | -8.615850e-47 |
| Msa0673460 | Msa1345920 | 0.804428 | 2.170527e-49 | -8.615850e-47 |
| Msa0688270 | Msa1345920 | 0.809212 | 2.115875e-50 | -8.615850e-47 |
| Msa0690440 | Msa1345920 | 0.802232 | 6.185741e-49 | -8.615850e-47 |
| Msa0766630 | Msa1345920 | 0.809074 | 2.264252e-50 | -8.615850e-47 |
| Msa0142220 | Msa1345920 | 0.814021 | 1.904774e-51 | -8.615850e-47 |
| Msa0188830 | Msa1345920 | 0.808443 | 3.088999e-50 | -8.615850e-47 |
| Msa1228590 | Msa1345920 | 0.801597 | 8.353687e-49 | -8.615850e-47 |
| Msa1343760 | Msa1345920 | 0.800427 | 1.448574e-48 | -8.615850e-47 |
| Msa0371730 | Msa1345920 | 0.809098 | 2.237471e-50 | -8.615850e-47 |
| Msa1014540 | Msa1345920 | 0.807289 | 5.434762e-50 | -8.615850e-47 |
| Msa1082500 | Msa1345920 | 0.815378 | 9.535375e-52 | -8.615850e-47 |
| Msa1083840 | Msa1345920 | 0.800038 | 1.738353e-48 | -8.615850e-47 |
| Msa0270520 | Msa1345920 | 0.804738 | 1.870080e-49 | -8.615850e-47 |
| Msa0309020 | Msa1345920 | 0.814272 | 1.676647e-51 | -8.615850e-47 |
| Msa1135220 | Msa1345920 | 0.816472 | 5.434580e-52 | -8.615850e-47 |
| Msa0541130 | Msa1345920 | 0.825380 | 4.837682e-54 | -8.615850e-47 |
| Msa0553460 | Msa1345920 | 0.809737 | 1.631880e-50 | -8.615850e-47 |
| Msa0885280 | Msa1345920 | 0.801620 | 8.262453e-49 | -8.615850e-47 |
| Msa0901400 | Msa1345920 | 0.807329 | 5.330384e-50 | -8.615850e-47 |
| Msa0935910 | Msa1345920 | 0.819212 | 1.307617e-52 | -8.615850e-47 |
| Msa0964220 | Msa1345920 | 0.800627 | 1.318650e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1345920 | MtrunA17_Chr1g0210141 | 93.893 | 131 | 7 | 1 | 1 | 130 | 1 | 131 | 9.11e-88 | 251 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACAAATTTGTTAGCCAAAT+AGG | 0.230270 | tig0007816:-7867 | None:intergenic |
| ATCAATGTGTTTGAAACTAT+TGG | 0.284145 | tig0007816:-7742 | None:intergenic |
| GCTCTGGTTCGCCACCTATT+TGG | 0.320551 | tig0007816:+7853 | Msa1345920:CDS |
| TGGTTTCAACTAAATCTTCA+TGG | 0.350113 | tig0007816:-7722 | None:intergenic |
| CACATTGATGTTACAAATGA+TGG | 0.350128 | tig0007816:+7756 | Msa1345920:CDS |
| TGAATTCAAATCCTAGTGTT+TGG | 0.353031 | tig0007816:+7625 | Msa1345920:CDS |
| TTGCAGCAAGAATGAACAAT+AGG | 0.400328 | tig0007816:+8084 | Msa1345920:intron |
| CTTCATTTCTTATTCACCAC+TGG | 0.403164 | tig0007816:-8125 | None:intergenic |
| CTTTCTATGCTGAGATTAAG+AGG | 0.404596 | tig0007816:+7673 | Msa1345920:CDS |
| CTATTTGGCTAACAAATTTG+TGG | 0.413481 | tig0007816:+7868 | Msa1345920:CDS |
| TACAAGAAAATTCTATTTGT+GGG | 0.427304 | tig0007816:+7821 | Msa1345920:CDS |
| TGGAGGAATGGTAAAGGTAC+TGG | 0.427886 | tig0007816:+7888 | Msa1345920:CDS |
| TTGTGGGAAACAGATAGCTC+TGG | 0.484793 | tig0007816:+7837 | Msa1345920:CDS |
| CTACAAGAAAATTCTATTTG+TGG | 0.487604 | tig0007816:+7820 | Msa1345920:CDS |
| CTAACAAATTTGTGGAGGAA+TGG | 0.499619 | tig0007816:+7876 | Msa1345920:CDS |
| TTACAAATGATGGTTCAAAC+AGG | 0.502417 | tig0007816:+7766 | Msa1345920:CDS |
| CATTCCTCAAGTTTCATGCA+AGG | 0.516692 | tig0007816:+7917 | Msa1345920:CDS |
| TCTGTCTCAAACCAAACACT+AGG | 0.561762 | tig0007816:-7636 | None:intergenic |
| GGTCTTCAGATGTTAACTGC+AGG | 0.570063 | tig0007816:-7701 | None:intergenic |
| TTTGGCTAACAAATTTGTGG+AGG | 0.590197 | tig0007816:+7871 | Msa1345920:CDS |
| CAAGAATGAACAATAGGACG+AGG | 0.601849 | tig0007816:+8090 | Msa1345920:CDS |
| AATTTGTGGAGGAATGGTAA+AGG | 0.605795 | tig0007816:+7882 | Msa1345920:CDS |
| TGCAAGGAAAATCAAAGACC+AGG | 0.622181 | tig0007816:+7933 | Msa1345920:CDS |
| AAATTTGTTAGCCAAATAGG+TGG | 0.659873 | tig0007816:-7864 | None:intergenic |
| GAGGAAAATATATAGACCAG+TGG | 0.692967 | tig0007816:+8109 | Msa1345920:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TACAAGAAAATTCTATTTGT+GGG | + | tig0007816:7821-7840 | Msa1345920:CDS | 20.0% |
| !! | TTCACAAATTTGTAAATTAG+TGG | + | tig0007816:7995-8014 | Msa1345920:intron | 20.0% |
| !! | ATCAATGTGTTTGAAACTAT+TGG | - | tig0007816:7745-7764 | None:intergenic | 25.0% |
| !! | CACAAATAGAATTTTCTTGT+AGG | - | tig0007816:7822-7841 | None:intergenic | 25.0% |
| ! | CTACAAGAAAATTCTATTTG+TGG | + | tig0007816:7820-7839 | Msa1345920:CDS | 25.0% |
| TGAATTCAAATCCTAGTGTT+TGG | + | tig0007816:7625-7644 | Msa1345920:CDS | 30.0% | |
| TGGTTTCAACTAAATCTTCA+TGG | - | tig0007816:7725-7744 | None:intergenic | 30.0% | |
| CACATTGATGTTACAAATGA+TGG | + | tig0007816:7756-7775 | Msa1345920:CDS | 30.0% | |
| TTACAAATGATGGTTCAAAC+AGG | + | tig0007816:7766-7785 | Msa1345920:CDS | 30.0% | |
| !! | AAATTTGTTAGCCAAATAGG+TGG | - | tig0007816:7867-7886 | None:intergenic | 30.0% |
| !! | CACAAATTTGTTAGCCAAAT+AGG | - | tig0007816:7870-7889 | None:intergenic | 30.0% |
| CTATTTGGCTAACAAATTTG+TGG | + | tig0007816:7868-7887 | Msa1345920:CDS | 30.0% | |
| TAAGAGATCAAAATTGTACC+TGG | - | tig0007816:7954-7973 | None:intergenic | 30.0% | |
| CTTTCTATGCTGAGATTAAG+AGG | + | tig0007816:7673-7692 | Msa1345920:CDS | 35.0% | |
| TTTGGCTAACAAATTTGTGG+AGG | + | tig0007816:7871-7890 | Msa1345920:CDS | 35.0% | |
| CTAACAAATTTGTGGAGGAA+TGG | + | tig0007816:7876-7895 | Msa1345920:CDS | 35.0% | |
| AATTTGTGGAGGAATGGTAA+AGG | + | tig0007816:7882-7901 | Msa1345920:CDS | 35.0% | |
| ! | TTTTCCTTGCATGAAACTTG+AGG | - | tig0007816:7924-7943 | None:intergenic | 35.0% |
| TTGCAGCAAGAATGAACAAT+AGG | + | tig0007816:8084-8103 | Msa1345920:intron | 35.0% | |
| GAGGAAAATATATAGACCAG+TGG | + | tig0007816:8109-8128 | Msa1345920:CDS | 35.0% | |
| TCTGTCTCAAACCAAACACT+AGG | - | tig0007816:7639-7658 | None:intergenic | 40.0% | |
| CATTCCTCAAGTTTCATGCA+AGG | + | tig0007816:7917-7936 | Msa1345920:CDS | 40.0% | |
| TGCAAGGAAAATCAAAGACC+AGG | + | tig0007816:7933-7952 | Msa1345920:CDS | 40.0% | |
| CAAGAATGAACAATAGGACG+AGG | + | tig0007816:8090-8109 | Msa1345920:CDS | 40.0% | |
| GGTCTTCAGATGTTAACTGC+AGG | - | tig0007816:7704-7723 | None:intergenic | 45.0% | |
| TTGTGGGAAACAGATAGCTC+TGG | + | tig0007816:7837-7856 | Msa1345920:CDS | 45.0% | |
| ! | TGGAGGAATGGTAAAGGTAC+TGG | + | tig0007816:7888-7907 | Msa1345920:CDS | 45.0% |
| ! | GCTCTGGTTCGCCACCTATT+TGG | + | tig0007816:7853-7872 | Msa1345920:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0007816 | gene | 7618 | 8145 | 7618 | ID=Msa1345920;Name=Msa1345920 |
| tig0007816 | mRNA | 7618 | 8145 | 7618 | ID=Msa1345920-mRNA-1;Parent=Msa1345920;Name=Msa1345920-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0|0|0|1|1|1|2|0|130 |
| tig0007816 | exon | 7618 | 7954 | 7618 | ID=Msa1345920-mRNA-1:exon:5241;Parent=Msa1345920-mRNA-1 |
| tig0007816 | exon | 8090 | 8145 | 8090 | ID=Msa1345920-mRNA-1:exon:5242;Parent=Msa1345920-mRNA-1 |
| tig0007816 | CDS | 7618 | 7954 | 7618 | ID=Msa1345920-mRNA-1:cds;Parent=Msa1345920-mRNA-1 |
| tig0007816 | CDS | 8090 | 8145 | 8090 | ID=Msa1345920-mRNA-1:cds;Parent=Msa1345920-mRNA-1 |
| Gene Sequence |
| Protein sequence |
