Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0901400 | KEH27251.1 | 78.095 | 105 | 18 | 1 | 1 | 100 | 697 | 801 | 5.05e-44 | 163 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0901400 | A0A072UCR4 | 78.095 | 105 | 18 | 1 | 1 | 100 | 697 | 801 | 2.41e-44 | 163 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0003890 | Msa0901400 | 0.806100 | 9.692345e-50 | -8.615850e-47 |
| Msa0006110 | Msa0901400 | 0.803307 | 3.710670e-49 | -8.615850e-47 |
| Msa0036140 | Msa0901400 | 0.819728 | 9.972135e-53 | -8.615850e-47 |
| Msa0044260 | Msa0901400 | 0.803251 | 3.811861e-49 | -8.615850e-47 |
| Msa0045790 | Msa0901400 | 0.817712 | 2.860203e-52 | -8.615850e-47 |
| Msa0046160 | Msa0901400 | 0.818274 | 2.135292e-52 | -8.615850e-47 |
| Msa0047110 | Msa0901400 | 0.818226 | 2.189630e-52 | -8.615850e-47 |
| Msa0054480 | Msa0901400 | 0.819611 | 1.060374e-52 | -8.615850e-47 |
| Msa0077400 | Msa0901400 | 0.800907 | 1.156029e-48 | -8.615850e-47 |
| Msa0080090 | Msa0901400 | 0.808100 | 3.655898e-50 | -8.615850e-47 |
| Msa0084380 | Msa0901400 | 0.813261 | 2.799087e-51 | -8.615850e-47 |
| Msa0114060 | Msa0901400 | 0.809449 | 1.881744e-50 | -8.615850e-47 |
| Msa0119960 | Msa0901400 | 0.803010 | 4.275680e-49 | -8.615850e-47 |
| Msa0616160 | Msa0901400 | 0.812329 | 4.479256e-51 | -8.615850e-47 |
| Msa0623070 | Msa0901400 | 0.811885 | 5.596754e-51 | -8.615850e-47 |
| Msa0625720 | Msa0901400 | 0.811171 | 7.999874e-51 | -8.615850e-47 |
| Msa0631360 | Msa0901400 | 0.833387 | 5.491433e-56 | -8.615850e-47 |
| Msa0657970 | Msa0901400 | 0.822911 | 1.838357e-53 | -8.615850e-47 |
| Msa0661650 | Msa0901400 | 0.803653 | 3.146341e-49 | -8.615850e-47 |
| Msa0663390 | Msa0901400 | 0.801978 | 6.977779e-49 | -8.615850e-47 |
| Msa0673450 | Msa0901400 | 0.810232 | 1.276986e-50 | -8.615850e-47 |
| Msa0673460 | Msa0901400 | 0.808282 | 3.343192e-50 | -8.615850e-47 |
| Msa0678930 | Msa0901400 | 0.801185 | 1.014446e-48 | -8.615850e-47 |
| Msa0681250 | Msa0901400 | 0.813720 | 2.219092e-51 | -8.615850e-47 |
| Msa0681270 | Msa0901400 | 0.814322 | 1.634168e-51 | -8.615850e-47 |
| Msa0688270 | Msa0901400 | 0.814765 | 1.304267e-51 | -8.615850e-47 |
| Msa0697030 | Msa0901400 | 0.819617 | 1.057127e-52 | -8.615850e-47 |
| Msa0697040 | Msa0901400 | 0.832203 | 1.080551e-55 | -8.615850e-47 |
| Msa0703780 | Msa0901400 | 0.806162 | 9.405305e-50 | -8.615850e-47 |
| Msa0724210 | Msa0901400 | 0.804321 | 2.284751e-49 | -8.615850e-47 |
| Msa0731940 | Msa0901400 | 0.823716 | 1.192266e-53 | -8.615850e-47 |
| Msa0740510 | Msa0901400 | 0.815833 | 7.550226e-52 | -8.615850e-47 |
| Msa0753720 | Msa0901400 | 0.805624 | 1.220220e-49 | -8.615850e-47 |
| Msa0760130 | Msa0901400 | 0.806703 | 7.231640e-50 | -8.615850e-47 |
| Msa0766630 | Msa0901400 | 0.823110 | 1.652667e-53 | -8.615850e-47 |
| Msa0767300 | Msa0901400 | 0.802199 | 6.282124e-49 | -8.615850e-47 |
| Msa0769920 | Msa0901400 | 0.804169 | 2.458168e-49 | -8.615850e-47 |
| Msa0790600 | Msa0901400 | 0.809534 | 1.804039e-50 | -8.615850e-47 |
| Msa0810220 | Msa0901400 | 0.809533 | 1.805033e-50 | -8.615850e-47 |
| Msa0820770 | Msa0901400 | 0.819868 | 9.262056e-53 | -8.615850e-47 |
| Msa0820850 | Msa0901400 | 0.811623 | 6.380808e-51 | -8.615850e-47 |
| Msa0825340 | Msa0901400 | 0.805827 | 1.106361e-49 | -8.615850e-47 |
| Msa0831110 | Msa0901400 | 0.800157 | 1.643894e-48 | -8.615850e-47 |
| Msa0832340 | Msa0901400 | 0.803328 | 3.673264e-49 | -8.615850e-47 |
| Msa0131770 | Msa0901400 | 0.804511 | 2.086073e-49 | -8.615850e-47 |
| Msa0142220 | Msa0901400 | 0.815065 | 1.119134e-51 | -8.615850e-47 |
| Msa0165210 | Msa0901400 | 0.801404 | 9.149329e-49 | -8.615850e-47 |
| Msa0188830 | Msa0901400 | 0.803945 | 2.736050e-49 | -8.615850e-47 |
| Msa0188900 | Msa0901400 | 0.800707 | 1.270151e-48 | -8.615850e-47 |
| Msa0211150 | Msa0901400 | 0.807811 | 4.211711e-50 | -8.615850e-47 |
| Msa0212990 | Msa0901400 | 0.801604 | 8.327288e-49 | -8.615850e-47 |
| Msa0214300 | Msa0901400 | 0.809666 | 1.690673e-50 | -8.615850e-47 |
| Msa0223010 | Msa0901400 | 0.810277 | 1.248504e-50 | -8.615850e-47 |
| Msa0379640 | Msa0901400 | 0.811470 | 6.888724e-51 | -8.615850e-47 |
| Msa0386090 | Msa0901400 | 0.806882 | 6.629516e-50 | -8.615850e-47 |
| Msa0402290 | Msa0901400 | 0.810274 | 1.250277e-50 | -8.615850e-47 |
| Msa0411390 | Msa0901400 | 0.824054 | 9.935278e-54 | -8.615850e-47 |
| Msa0428080 | Msa0901400 | 0.805865 | 1.085972e-49 | -8.615850e-47 |
| Msa0437990 | Msa0901400 | 0.804726 | 1.880794e-49 | -8.615850e-47 |
| Msa0440400 | Msa0901400 | 0.814974 | 1.171845e-51 | -8.615850e-47 |
| Msa0440750 | Msa0901400 | 0.809365 | 1.961632e-50 | -8.615850e-47 |
| Msa0450790 | Msa0901400 | 0.802821 | 4.677895e-49 | -8.615850e-47 |
| Msa0455760 | Msa0901400 | 0.824045 | 9.986125e-54 | -8.615850e-47 |
| Msa0464680 | Msa0901400 | 0.805625 | 1.219723e-49 | -8.615850e-47 |
| Msa0260680 | Msa0901400 | 0.812943 | 3.287569e-51 | -8.615850e-47 |
| Msa0265670 | Msa0901400 | 0.815336 | 9.740216e-52 | -8.615850e-47 |
| Msa0273100 | Msa0901400 | 0.809444 | 1.886335e-50 | -8.615850e-47 |
| Msa0280090 | Msa0901400 | 0.824137 | 9.499915e-54 | -8.615850e-47 |
| Msa0285450 | Msa0901400 | 0.806236 | 9.071375e-50 | -8.615850e-47 |
| Msa0287410 | Msa0901400 | 0.800371 | 1.486916e-48 | -8.615850e-47 |
| Msa0319170 | Msa0901400 | 0.811236 | 7.743229e-51 | -8.615850e-47 |
| Msa0326940 | Msa0901400 | 0.805567 | 1.253962e-49 | -8.615850e-47 |
| Msa0339620 | Msa0901400 | 0.808057 | 3.734646e-50 | -8.615850e-47 |
| Msa0355220 | Msa0901400 | 0.804817 | 1.800879e-49 | -8.615850e-47 |
| Msa0357340 | Msa0901400 | 0.814592 | 1.424041e-51 | -8.615850e-47 |
| Msa0363790 | Msa0901400 | 0.833829 | 4.259910e-56 | -8.615850e-47 |
| Msa0486690 | Msa0901400 | 0.807985 | 3.868820e-50 | -8.615850e-47 |
| Msa0513490 | Msa0901400 | 0.804296 | 2.312114e-49 | -8.615850e-47 |
| Msa0516170 | Msa0901400 | 0.808298 | 3.317190e-50 | -8.615850e-47 |
| Msa0528950 | Msa0901400 | 0.822894 | 1.855676e-53 | -8.615850e-47 |
| Msa0529890 | Msa0901400 | 0.812214 | 4.743823e-51 | -8.615850e-47 |
| Msa0532350 | Msa0901400 | 0.803595 | 3.234441e-49 | -8.615850e-47 |
| Msa0537500 | Msa0901400 | 0.810729 | 9.970646e-51 | -8.615850e-47 |
| Msa0541130 | Msa0901400 | 0.812213 | 4.747595e-51 | -8.615850e-47 |
| Msa0545210 | Msa0901400 | 0.807331 | 5.325872e-50 | -8.615850e-47 |
| Msa0585560 | Msa0901400 | 0.804957 | 1.683423e-49 | -8.615850e-47 |
| Msa0855760 | Msa0901400 | 0.815312 | 9.861027e-52 | -8.615850e-47 |
| Msa0858130 | Msa0901400 | 0.811894 | 5.571031e-51 | -8.615850e-47 |
| Msa0861090 | Msa0901400 | 0.812521 | 4.065098e-51 | -8.615850e-47 |
| Msa0861130 | Msa0901400 | 0.811706 | 6.122860e-51 | -8.615850e-47 |
| Msa0880950 | Msa0901400 | 0.824083 | 9.782816e-54 | -8.615850e-47 |
| Msa0885280 | Msa0901400 | 0.817245 | 3.645341e-52 | -8.615850e-47 |
| Msa0898750 | Msa0901400 | 0.817639 | 2.971464e-52 | -8.615850e-47 |
| Msa0901400 | Msa0923010 | 0.813158 | 2.949316e-51 | -8.615850e-47 |
| Msa0901400 | Msa0925810 | 0.848802 | 4.857983e-60 | -8.615850e-47 |
| Msa0901400 | Msa0926890 | 0.802407 | 5.692897e-49 | -8.615850e-47 |
| Msa0901400 | Msa0928130 | 0.817549 | 3.112878e-52 | -8.615850e-47 |
| Msa0901400 | Msa0935910 | 0.826147 | 3.182131e-54 | -8.615850e-47 |
| Msa0901400 | Msa0964310 | 0.814203 | 1.736744e-51 | -8.615850e-47 |
| Msa0901400 | Msa0965610 | 0.801372 | 9.287726e-49 | -8.615850e-47 |
| Msa0901400 | Msa0969520 | 0.809268 | 2.057570e-50 | -8.615850e-47 |
| Msa0901400 | Msa0969600 | 0.813113 | 3.016034e-51 | -8.615850e-47 |
| Msa0901400 | Msa0973600 | 0.825686 | 4.093886e-54 | -8.615850e-47 |
| Msa0901400 | Msa0998740 | 0.807102 | 5.956167e-50 | -8.615850e-47 |
| Msa0901400 | Msa1003620 | 0.800966 | 1.124705e-48 | -8.615850e-47 |
| Msa0901400 | Msa1004840 | 0.818208 | 2.209578e-52 | -8.615850e-47 |
| Msa0901400 | Msa1014540 | 0.828167 | 1.045041e-54 | -8.615850e-47 |
| Msa0901400 | Msa1016320 | 0.803938 | 2.745617e-49 | -8.615850e-47 |
| Msa0901400 | Msa1018980 | 0.809037 | 2.306579e-50 | -8.615850e-47 |
| Msa0901400 | Msa1019000 | 0.822848 | 1.902103e-53 | -8.615850e-47 |
| Msa0901400 | Msa1019150 | 0.800807 | 1.212164e-48 | -8.615850e-47 |
| Msa0901400 | Msa1023950 | 0.845732 | 3.379520e-59 | -8.615850e-47 |
| Msa0901400 | Msa1030970 | 0.814257 | 1.689667e-51 | -8.615850e-47 |
| Msa0901400 | Msa1075540 | 0.813873 | 2.053615e-51 | -8.615850e-47 |
| Msa0901400 | Msa1075650 | 0.840879 | 6.664597e-58 | -8.615850e-47 |
| Msa0901400 | Msa1076700 | 0.822664 | 2.098963e-53 | -8.615850e-47 |
| Msa0901400 | Msa1082500 | 0.829392 | 5.281078e-55 | -8.615850e-47 |
| Msa0901400 | Msa1083840 | 0.825948 | 3.548834e-54 | -8.615850e-47 |
| Msa0901400 | Msa1098400 | 0.819339 | 1.223449e-52 | -8.615850e-47 |
| Msa0901400 | Msa1098420 | 0.812358 | 4.413567e-51 | -8.615850e-47 |
| Msa0901400 | Msa1100050 | 0.805064 | 1.598405e-49 | -8.615850e-47 |
| Msa0901400 | Msa1104090 | 0.804025 | 2.633507e-49 | -8.615850e-47 |
| Msa0901400 | Msa1108760 | 0.810875 | 9.274455e-51 | -8.615850e-47 |
| Msa0901400 | Msa1121490 | 0.807968 | 3.900956e-50 | -8.615850e-47 |
| Msa0901400 | Msa1122520 | 0.808681 | 2.748005e-50 | -8.615850e-47 |
| Msa0901400 | Msa1131220 | 0.804449 | 2.148323e-49 | -8.615850e-47 |
| Msa0901400 | Msa1147710 | 0.807926 | 3.981893e-50 | -8.615850e-47 |
| Msa0901400 | Msa1147740 | 0.806713 | 7.197618e-50 | -8.615850e-47 |
| Msa0901400 | Msa1154280 | 0.805874 | 1.081265e-49 | -8.615850e-47 |
| Msa0901400 | Msa1162780 | 0.800181 | 1.625806e-48 | -8.615850e-47 |
| Msa0901400 | Msa1174040 | 0.826343 | 2.858444e-54 | -8.615850e-47 |
| Msa0901400 | Msa1204530 | 0.803533 | 3.331776e-49 | -8.615850e-47 |
| Msa0901400 | Msa1249490 | 0.800608 | 1.330704e-48 | -8.615850e-47 |
| Msa0901400 | Msa1253000 | 0.833400 | 5.451920e-56 | -8.615850e-47 |
| Msa0901400 | Msa1277660 | 0.822338 | 2.499258e-53 | -8.615850e-47 |
| Msa0901400 | Msa1284680 | 0.804824 | 1.794062e-49 | -8.615850e-47 |
| Msa0901400 | Msa1331000 | 0.830889 | 2.277442e-55 | -8.615850e-47 |
| Msa0901400 | Msa1333410 | 0.800546 | 1.370101e-48 | -8.615850e-47 |
| Msa0901400 | Msa1342840 | 0.808886 | 2.484606e-50 | -8.615850e-47 |
| Msa0901400 | Msa1343760 | 0.801522 | 8.655108e-49 | -8.615850e-47 |
| Msa0901400 | Msa1345920 | 0.807329 | 5.330384e-50 | -8.615850e-47 |
| Msa0901400 | Msa1353470 | 0.815137 | 1.078354e-51 | -8.615850e-47 |
| Msa0901400 | Msa1372760 | 0.805612 | 1.227483e-49 | -8.615850e-47 |
| Msa0901400 | Msa1373030 | 0.811937 | 5.453575e-51 | -8.615850e-47 |
| Msa0901400 | Msa1378040 | 0.806143 | 9.492626e-50 | -8.615850e-47 |
| Msa0901400 | Msa1394380 | 0.816919 | 4.314540e-52 | -8.615850e-47 |
| Msa0901400 | Msa1397860 | 0.807151 | 5.813113e-50 | -8.615850e-47 |
| Msa0901400 | Msa1408340 | 0.804831 | 1.788624e-49 | -8.615850e-47 |
| Msa0901400 | Msa1409700 | 0.816783 | 4.628312e-52 | -8.615850e-47 |
| Msa0901400 | Msa1410680 | 0.810282 | 1.245360e-50 | -8.615850e-47 |
| Msa0901400 | Msa1427230 | 0.819405 | 1.181931e-52 | -8.615850e-47 |
| Msa0901400 | Msa1429900 | 0.801682 | 8.023275e-49 | -8.615850e-47 |
| Msa0901400 | Msa1436480 | 0.820996 | 5.105696e-53 | -8.615850e-47 |
| Msa0901400 | Msa1440560 | 0.806691 | 7.274303e-50 | -8.615850e-47 |
| Msa0901400 | Msa1452240 | 0.811784 | 5.886823e-51 | -8.615850e-47 |
| Msa0901400 | Msa1460830 | 0.808653 | 2.787052e-50 | -8.615850e-47 |
| Msa0901400 | Msa1463900 | 0.803714 | 3.055636e-49 | -8.615850e-47 |
| Msa0901400 | Msa1466540 | 0.829045 | 6.411722e-55 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0901400 | MtrunA17_Chr6g0487991 | 78.095 | 105 | 18 | 1 | 1 | 100 | 762 | 866 | 5.63e-48 | 162 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTTCTATTTCCTCGGCTTT+CGG | 0.284663 | 6_1:+98099842 | None:intergenic |
| TCTGATGTATTCTTAATATA+AGG | 0.320184 | 6_1:+98099969 | None:intergenic |
| GATGTATACTTGTCCTGAAC+AGG | 0.426482 | 6_1:+98099864 | None:intergenic |
| CTGAACAGGAGCATCAAAAC+TGG | 0.438709 | 6_1:+98099878 | None:intergenic |
| AGCCGACATTTCTATTTCCT+CGG | 0.444211 | 6_1:+98099835 | None:intergenic |
| TGATTCTGATAGCTACGATC+TGG | 0.490408 | 6_1:-98099790 | Msa0901400:CDS |
| ATTAAGCTACAGAACGTTGA+AGG | 0.508639 | 6_1:-98100024 | Msa0901400:CDS |
| AGATGATGTTTCAACTTCAG+TGG | 0.545104 | 6_1:-98099949 | Msa0901400:CDS |
| GATGATGTTTCAACTTCAGT+GGG | 0.546176 | 6_1:-98099948 | Msa0901400:CDS |
| CTGCACACAATCTTTCACTC+TGG | 0.560209 | 6_1:+98099913 | None:intergenic |
| GATGTATTCTTAATATAAGG+TGG | 0.589065 | 6_1:+98099972 | None:intergenic |
| AGCCGAGGAAATAGAAATGT+CGG | 0.590279 | 6_1:-98099837 | Msa0901400:CDS |
| AGCTACAGAACGTTGAAGGG+TGG | 0.614968 | 6_1:-98100020 | Msa0901400:CDS |
| TTAAGCTACAGAACGTTGAA+GGG | 0.632705 | 6_1:-98100023 | Msa0901400:CDS |
| AAGTATACATCCGAAAGCCG+AGG | 0.674180 | 6_1:-98099852 | Msa0901400:CDS |
| GTGAAAGATTGTGTGCAGTG+AGG | 0.767939 | 6_1:-98099908 | Msa0901400:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCTGATGTATTCTTAATATA+AGG | + | chr6_1:98099812-98099831 | None:intergenic | 20.0% |
| ! | GATGTATTCTTAATATAAGG+TGG | + | chr6_1:98099809-98099828 | None:intergenic | 25.0% |
| ATTAAGCTACAGAACGTTGA+AGG | - | chr6_1:98099754-98099773 | Msa0901400:CDS | 35.0% | |
| TTAAGCTACAGAACGTTGAA+GGG | - | chr6_1:98099755-98099774 | Msa0901400:CDS | 35.0% | |
| AGATGATGTTTCAACTTCAG+TGG | - | chr6_1:98099829-98099848 | Msa0901400:CDS | 35.0% | |
| GATGATGTTTCAACTTCAGT+GGG | - | chr6_1:98099830-98099849 | Msa0901400:CDS | 35.0% | |
| ATTTCTATTTCCTCGGCTTT+CGG | + | chr6_1:98099939-98099958 | None:intergenic | 35.0% | |
| ! | AAGGGTGGTTTTCGCAATAA+AGG | - | chr6_1:98099773-98099792 | Msa0901400:CDS | 40.0% |
| GATGTATACTTGTCCTGAAC+AGG | + | chr6_1:98099917-98099936 | None:intergenic | 40.0% | |
| AGCCGAGGAAATAGAAATGT+CGG | - | chr6_1:98099941-98099960 | Msa0901400:CDS | 40.0% | |
| AGCCGACATTTCTATTTCCT+CGG | + | chr6_1:98099946-98099965 | None:intergenic | 40.0% | |
| TGATTCTGATAGCTACGATC+TGG | - | chr6_1:98099988-98100007 | Msa0901400:CDS | 40.0% | |
| CTGCACACAATCTTTCACTC+TGG | + | chr6_1:98099868-98099887 | None:intergenic | 45.0% | |
| GTGAAAGATTGTGTGCAGTG+AGG | - | chr6_1:98099870-98099889 | Msa0901400:CDS | 45.0% | |
| !! | CTGAACAGGAGCATCAAAAC+TGG | + | chr6_1:98099903-98099922 | None:intergenic | 45.0% |
| !! | CAGTTTTGATGCTCCTGTTC+AGG | - | chr6_1:98099901-98099920 | Msa0901400:CDS | 45.0% |
| AAGTATACATCCGAAAGCCG+AGG | - | chr6_1:98099926-98099945 | Msa0901400:CDS | 45.0% | |
| AGCTACAGAACGTTGAAGGG+TGG | - | chr6_1:98099758-98099777 | Msa0901400:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_1 | gene | 98099749 | 98100051 | 98099749 | ID=Msa0901400;Name=Msa0901400 |
| chr6_1 | mRNA | 98099749 | 98100051 | 98099749 | ID=Msa0901400-mRNA-1;Parent=Msa0901400;Name=Msa0901400-mRNA-1;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|100 |
| chr6_1 | exon | 98099749 | 98100051 | 98099749 | ID=Msa0901400-mRNA-1:exon:17279;Parent=Msa0901400-mRNA-1 |
| chr6_1 | CDS | 98099749 | 98100051 | 98099749 | ID=Msa0901400-mRNA-1:cds;Parent=Msa0901400-mRNA-1 |
| Gene Sequence |
| Protein sequence |
