AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1439410

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1439410 MtrunA17_Chr6g0456381 98.485 264 4 0 20 283 226 489 0.0 548
Msa1439410 MtrunA17_Chr6g0456401 98.106 264 5 0 20 283 226 489 0.0 548
Msa1439410 MtrunA17_Chr6g0456391 98.106 264 5 0 20 283 226 489 0.0 547
Msa1439410 MtrunA17_Chr7g0221391 79.545 264 54 0 20 283 228 491 1.54e-159 453
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1439410 AT3G30775.1 61.922 281 105 2 4 282 218 498 1.71e-121 357
Msa1439410 AT3G30775.2 61.922 281 105 2 4 282 218 498 1.71e-121 357
Msa1439410 AT5G38710.1 62.357 263 97 2 20 280 213 475 2.22e-120 353
Msa1439410 AT5G38710.2 62.205 254 94 2 20 271 213 466 3.16e-116 342

Find 94 sgRNAs with CRISPR-Local

Find 229 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTCTTCTTGTGGTGTTAA+TGG 0.140964 tig0028755:+34669 None:intergenic
GGTTGTGATCGTAATCTTAA+AGG 0.228265 tig0028755:-36841 Msa1439410:five_prime_UTR
TTACGTGGCATGCTTGTTTA+TGG 0.238176 tig0028755:-36889 Msa1439410:five_prime_UTR
TCTTGTGGTGTTAATGGTTC+TGG 0.257780 tig0028755:+34675 None:intergenic
TGGACTTGATTCTGAGAAAT+TGG 0.259093 tig0028755:+34719 None:intergenic
GACAAAGTTGTTGTAATCTT+TGG 0.266260 tig0028755:+34628 None:intergenic
CTGTCGTGGACGTTGGAATA+TGG 0.273143 tig0028755:-37056 Msa1439410:five_prime_UTR
AGTACATTGGCTGAGTCTTT+TGG 0.274273 tig0028755:-34354 Msa1439410:CDS
GAAACTCTCGACAACACTTT+AGG 0.315281 tig0028755:+37430 None:intergenic
GAAGGATGGGAGGAGTTTAA+TGG 0.324027 tig0028755:+37261 None:intergenic
GATGATAATCCTATTGTGTT+TGG 0.331772 tig0028755:-34498 Msa1439410:CDS
GCAGCAAAAGCATATGAAAT+TGG 0.349585 tig0028755:-33688 Msa1439410:CDS
TTTCTTCAGCTCTTCTCAAT+AGG 0.352369 tig0028755:+33527 None:intergenic
TGAAGAAAATAGAGGCCTTT+TGG 0.353136 tig0028755:-33512 Msa1439410:CDS
TGAATTGTTCCAAACACAAT+AGG 0.356564 tig0028755:+34489 None:intergenic
ATGGGATTTAAGTTGGTTAG+AGG 0.360764 tig0028755:-34396 Msa1439410:CDS
TTGCTTGAGAAGTTTGCTAA+TGG 0.363959 tig0028755:-34264 Msa1439410:CDS
GTTAGCAAGTATATGCCATT+TGG 0.380807 tig0028755:-33574 Msa1439410:CDS
GTTGTGATCGTAATCTTAAA+GGG 0.388070 tig0028755:-36840 Msa1439410:five_prime_UTR
TCATTTGGTTTGAGCAATGC+AGG 0.402184 tig0028755:-33604 Msa1439410:CDS
TTGCTGCTGGAAAGAGTATA+AGG 0.405411 tig0028755:-36930 Msa1439410:five_prime_UTR
ATAGTCTCTCAGCGTCGTTT+AGG 0.405775 tig0028755:+37145 None:intergenic
GAGAAGTTTGCTAATGGAAA+AGG 0.409585 tig0028755:-34258 Msa1439410:CDS
GACGGTTGTGGTGGTGGTGG+TGG 0.410895 tig0028755:+37192 None:intergenic
ATGTCAGATGCACTTTCATT+TGG 0.412207 tig0028755:-33619 Msa1439410:CDS
TGGTGTGACGGTTGTGGTGG+TGG 0.427223 tig0028755:+37186 None:intergenic
GTTCTGCATGCAACGGCTAT+AGG 0.438450 tig0028755:-37081 Msa1439410:five_prime_UTR
TTCCATGGTAAATTGAATGA+AGG 0.441489 tig0028755:+34755 None:intergenic
GCAATCAAAATTGTTACAAA+CGG 0.447102 tig0028755:-37028 Msa1439410:five_prime_UTR
TGTGACGGTTGTGGTGGTGG+TGG 0.448290 tig0028755:+37189 None:intergenic
TCTTCAATAGTATCATGAAT+TGG 0.450449 tig0028755:+34321 None:intergenic
TGTCGTGGACGTTGGAATAT+GGG 0.452589 tig0028755:-37055 Msa1439410:five_prime_UTR
AATTAGAAACTTACAGAAGA+TGG 0.452786 tig0028755:+36781 None:intergenic
AATTCCTATGGGATTTAAGT+TGG 0.453707 tig0028755:-34403 Msa1439410:CDS
CAATCAAAATTGTTACAAAC+GGG 0.455063 tig0028755:-37027 Msa1439410:five_prime_UTR
GCTACACACAACATTGAATC+AGG 0.457514 tig0028755:-34222 Msa1439410:intron
GGTCGAGATAAGACGGTTGT+TGG 0.459521 tig0028755:+37166 None:intergenic
GTTGTGCAAATTCAAGTTTG+TGG 0.466588 tig0028755:+33650 None:intergenic
CGGCAGCGGTGGCTGAAGGA+TGG 0.474169 tig0028755:+37247 None:intergenic
GGAGAAGATGCTATTGCTGC+TGG 0.477420 tig0028755:-36943 Msa1439410:five_prime_UTR
TGAAAATCACTTTCTTCTTG+TGG 0.479035 tig0028755:+34660 None:intergenic
ACCATCTCAACTGGTCCAAA+TGG 0.482748 tig0028755:+33559 None:intergenic
CTTGAATTTGCACAACTATG+TGG 0.482939 tig0028755:-33643 Msa1439410:CDS
ATAAGGTCTGTTAATGAAGC+TGG 0.484228 tig0028755:-36913 Msa1439410:five_prime_UTR
AACTTATTTGAAAGATGCTA+AGG 0.486569 tig0028755:-34466 Msa1439410:CDS
AAAGCATATGAAATTGGAAT+GGG 0.486854 tig0028755:-33682 Msa1439410:CDS
GGTAGAGAGTCTTGTTTCCA+TGG 0.489711 tig0028755:+34740 None:intergenic
GGCAGCGGTGGCTGAAGGAT+GGG 0.493133 tig0028755:+37248 None:intergenic
TGTACAGAAAAGAGCTTGGA+AGG 0.493267 tig0028755:-33356 Msa1439410:intron
TAAGGCATAACCATCTCAAC+TGG 0.497185 tig0028755:+33550 None:intergenic
GGGAGGAGTTTAATGGCTTG+TGG 0.505443 tig0028755:+37268 None:intergenic
AATCTTGAAAGATGCAGTTA+TGG 0.507538 tig0028755:-37004 Msa1439410:five_prime_UTR
TGTTCAGCATCAACCAATAG+TGG 0.510458 tig0028755:+34582 None:intergenic
TGAAGCAAACATGCCACTAT+TGG 0.511802 tig0028755:-34595 Msa1439410:CDS
CGTTGCATGCAGAACGGCTG+AGG 0.516590 tig0028755:+37089 None:intergenic
AAGGGAGGCGACGGCAGCGG+TGG 0.516875 tig0028755:+37236 None:intergenic
ATTCTTTCTAGTAATGCCAT+TGG 0.522940 tig0028755:+34809 None:intergenic
GAGAAGGGAGGCGACGGCAG+CGG 0.523270 tig0028755:+37233 None:intergenic
AGAAGAGCTGAAGAAAATAG+AGG 0.524857 tig0028755:-33520 Msa1439410:CDS
CATTTGGTTTGAGCAATGCA+GGG 0.525718 tig0028755:-33603 Msa1439410:CDS
TCAGCTCTTCTCAATAGGTA+AGG 0.526405 tig0028755:+33532 None:intergenic
CAAATCCAGAAGCAGCCAAA+AGG 0.529777 tig0028755:+33497 None:intergenic
GCCATTTGGACCAGTTGAGA+TGG 0.538971 tig0028755:-33560 Msa1439410:CDS
GGTTGTTGGTGTGACGGTTG+TGG 0.540727 tig0028755:+37180 None:intergenic
AGGTTGTTCTCTTTCGAGAA+GGG 0.540968 tig0028755:+37218 None:intergenic
AGGAGTTTAATGGCTTGTGG+TGG 0.541900 tig0028755:+37271 None:intergenic
TCCATGGTAAATTGAATGAA+GGG 0.543779 tig0028755:+34756 None:intergenic
GCGACGGCAGCGGTGGCTGA+AGG 0.544078 tig0028755:+37243 None:intergenic
AAAAGCATATGAAATTGGAA+TGG 0.545661 tig0028755:-33683 Msa1439410:CDS
TATGTGAAGTAATCAATAGC+TGG 0.545894 tig0028755:+34546 None:intergenic
GAATAAGTGATCTTCTAAGA+TGG 0.547341 tig0028755:-34790 Msa1439410:exon
ACCCTTCATTCAATTTACCA+TGG 0.548501 tig0028755:-34757 Msa1439410:CDS
GATTTGATAGACAACTCATA+AGG 0.552477 tig0028755:-33480 Msa1439410:intron
AAGGTTGTTCTCTTTCGAGA+AGG 0.560901 tig0028755:+37217 None:intergenic
CATATTCCAACGTCCACGAC+AGG 0.571109 tig0028755:+37057 None:intergenic
TCTTTCGAGAAGGGAGGCGA+CGG 0.578179 tig0028755:+37227 None:intergenic
GTTAATGAAGCTGGCTTACG+TGG 0.581537 tig0028755:-36904 Msa1439410:five_prime_UTR
AATCACTGCAATATGTCCAA+TGG 0.585286 tig0028755:-34825 Msa1439410:five_prime_UTR
ATAGGACCTGTCGTGGACGT+TGG 0.590338 tig0028755:-37063 Msa1439410:five_prime_UTR
AGCGGTGGCTGAAGGATGGG+AGG 0.592747 tig0028755:+37251 None:intergenic
TGTTGGTGTGACGGTTGTGG+TGG 0.605574 tig0028755:+37183 None:intergenic
CTAACCAACTTAAATCCCAT+AGG 0.606330 tig0028755:+34399 None:intergenic
TTGTTCTCTTTCGAGAAGGG+AGG 0.606843 tig0028755:+37221 None:intergenic
TCGTTTAGGTCGAGATAAGA+CGG 0.607879 tig0028755:+37159 None:intergenic
GATCTTATCAACGTGGACGT+TGG 0.615310 tig0028755:+37111 None:intergenic
CTCAGCCGTTCTGCATGCAA+CGG 0.616136 tig0028755:-37088 Msa1439410:five_prime_UTR
GAAGATGCACATGATAATGA+TGG 0.618481 tig0028755:-36862 Msa1439410:five_prime_UTR
AAGTGATTTGCTAACATCAA+CGG 0.622647 tig0028755:+36807 None:intergenic
TATGTCTACAGAAAGTACAT+TGG 0.622655 tig0028755:-34367 Msa1439410:CDS
TATAGCCGTTGCATGCAGAA+CGG 0.636543 tig0028755:+37083 None:intergenic
TAAGACGGTTGTTGGTGTGA+CGG 0.642129 tig0028755:+37174 None:intergenic
TAGAAACTTACAGAAGATGG+AGG 0.662988 tig0028755:+36784 None:intergenic
ACAGAAAAGAGCTTGGAAGG+AGG 0.680297 tig0028755:-33353 Msa1439410:intron
GGCTGAGGATCTTATCAACG+TGG 0.744582 tig0028755:+37104 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTATTATTATTATTATTA+TGG + tig0028755:34293-34312 None:intergenic 0.0%
!!! TAATAATAAATTAAATATTT+TGG - tig0028755:35072-35091 Msa1439410:intron 0.0%
!!! TTATATTTTATACATAATGT+TGG - tig0028755:35267-35286 Msa1439410:intron 10.0%
!!! ACATTTTCTTAAAATATTAT+CGG + tig0028755:36504-36523 None:intergenic 10.0%
!!! ACATTTTCTTAAAATATTAT+CGG + tig0028755:36504-36523 None:intergenic 10.0%
!!! AAATGAATTTTGAATTATTA+TGG - tig0028755:37384-37403 Msa1439410:five_prime_UTR 10.0%
!!! TTTTATTTAAAGAAGAAAAG+AGG - tig0028755:33908-33927 Msa1439410:intron 15.0%
!!! CTATTTTTTAAAAGCAAAAT+TGG - tig0028755:34054-34073 Msa1439410:intron 15.0%
!!! TGAAATGTGTTTTAATATAA+AGG - tig0028755:34248-34267 Msa1439410:CDS 15.0%
!!! TACTTTTCTAATTTTTTATG+TGG - tig0028755:34389-34408 Msa1439410:CDS 15.0%
!! TGTCTCATATTATATATTAT+TGG - tig0028755:34870-34889 Msa1439410:intron 15.0%
!!! TTGTACATTTTAATATGAAT+TGG + tig0028755:35557-35576 None:intergenic 15.0%
!!! TTGATTTTTTTTTATGTGAT+AGG - tig0028755:35708-35727 Msa1439410:intron 15.0%
!! ATTAGATATTTACATTCATT+AGG - tig0028755:36381-36400 Msa1439410:intron 15.0%
!! TTAGATATTTACATTCATTA+GGG - tig0028755:36382-36401 Msa1439410:intron 15.0%
!! AATTACATTAATTGAAATCT+TGG + tig0028755:36841-36860 None:intergenic 15.0%
!! TGTCTCAAAATAATTAAAAA+AGG + tig0028755:37147-37166 None:intergenic 15.0%
!!! AATTATTATGGTATTTTTGT+TGG - tig0028755:37396-37415 Msa1439410:five_prime_UTR 15.0%
!!! CAAATTTTCTTGTTTTTGAA+TGG - tig0028755:33233-33252 Msa1439410:three_prime_UTR 20.0%
!!! AGTATTAATTTTCATGATCA+TGG - tig0028755:33837-33856 Msa1439410:intron 20.0%
!! ACTTTAAATAAAGATTGAAG+AGG - tig0028755:34190-34209 Msa1439410:intron 20.0%
!! CATTTCACACAAAAAATATT+GGG + tig0028755:34235-34254 None:intergenic 20.0%
!! ACATTTCACACAAAAAATAT+TGG + tig0028755:34236-34255 None:intergenic 20.0%
!! TATTATTATGGTGTAAATGA+GGG + tig0028755:34281-34300 None:intergenic 20.0%
!! TTATTATTATGGTGTAAATG+AGG + tig0028755:34282-34301 None:intergenic 20.0%
!! TCATTATTTCCTTAACTATT+GGG + tig0028755:34421-34440 None:intergenic 20.0%
!! ATCATTATTTCCTTAACTAT+TGG + tig0028755:34422-34441 None:intergenic 20.0%
!! TAATAATACAAAGTAGTTGA+TGG + tig0028755:34533-34552 None:intergenic 20.0%
!!! TTTTTAAGTTGCAAAAATCT+TGG - tig0028755:34822-34841 Msa1439410:five_prime_UTR 20.0%
!!! AAATTTGAGTTTGGTATTTT+TGG - tig0028755:34963-34982 Msa1439410:intron 20.0%
!!! TTTGGTATTTTTGGATAAAA+GGG - tig0028755:34972-34991 Msa1439410:intron 20.0%
!!! AGTGTTTAGAAATTAGATTT+TGG - tig0028755:35349-35368 Msa1439410:intron 20.0%
!!! GAAATTAGATTTTGGTATTT+TGG - tig0028755:35357-35376 Msa1439410:intron 20.0%
!! AATAAAACTCTAGTAAGAAA+TGG + tig0028755:35419-35438 None:intergenic 20.0%
!!! TCAAAGTGTTTATACTAAAT+TGG - tig0028755:35449-35468 Msa1439410:intron 20.0%
!! ATAAATGTAACCACATATAT+CGG - tig0028755:36726-36745 Msa1439410:intron 20.0%
!!! TGTAATTTTTTTTGTTGTGT+AGG - tig0028755:36854-36873 Msa1439410:five_prime_UTR 20.0%
!!! GTAATTTTTTTTGTTGTGTA+GGG - tig0028755:36855-36874 Msa1439410:five_prime_UTR 20.0%
! GCAATCAAAATTGTTACAAA+CGG - tig0028755:33547-33566 Msa1439410:CDS 25.0%
! CAATCAAAATTGTTACAAAC+GGG - tig0028755:33548-33567 Msa1439410:CDS 25.0%
!! TTTTATGAACATTTTTGTGC+TGG - tig0028755:33611-33630 Msa1439410:CDS 25.0%
! AATTAGAAACTTACAGAAGA+TGG + tig0028755:33797-33816 None:intergenic 25.0%
!! TTAAAGAAGAAAAGAGGTTT+TGG - tig0028755:33914-33933 Msa1439410:intron 25.0%
!!! AGGTTTTGGTCAAATTAATT+TGG - tig0028755:33928-33947 Msa1439410:intron 25.0%
! CATTATTTCCTTAACTATTG+GGG + tig0028755:34420-34439 None:intergenic 25.0%
! TGTATGTCTTGAAAAACATA+AGG - tig0028755:34447-34466 Msa1439410:CDS 25.0%
!! AGTGTTTAGAAATTTGAGTT+TGG - tig0028755:34954-34973 Msa1439410:intron 25.0%
!!! GTTTGGTATTTTTGGATAAA+AGG - tig0028755:34971-34990 Msa1439410:intron 25.0%
! AAAAGGGTTAATCAGTAAAT+TGG - tig0028755:34988-35007 Msa1439410:intron 25.0%
! ACCATTTCTAATGTAATTAG+AGG - tig0028755:35017-35036 Msa1439410:intron 25.0%
! ACCTCTAATTACATTAGAAA+TGG + tig0028755:35021-35040 None:intergenic 25.0%
!!! TGTTACTGTTTTAGACAAAA+TGG - tig0028755:35169-35188 Msa1439410:intron 25.0%
!!! ATTTTGGTATTTTGGACAAA+AGG - tig0028755:35365-35384 Msa1439410:intron 25.0%
!!! TTTTGGTATTTTGGACAAAA+GGG - tig0028755:35366-35385 Msa1439410:intron 25.0%
! GTGTCAAATGCAAAAAATTA+TGG + tig0028755:35491-35510 None:intergenic 25.0%
!!! TTTTTTGTAGTGTGGTATAA+TGG + tig0028755:35879-35898 None:intergenic 25.0%
! AACTTATTTGAAAGATGCTA+AGG - tig0028755:36109-36128 Msa1439410:intron 25.0%
! TGAAAAAATTGGAATTCCTA+TGG - tig0028755:36160-36179 Msa1439410:intron 25.0%
! GAAAAAATTGGAATTCCTAT+GGG - tig0028755:36161-36180 Msa1439410:intron 25.0%
! TCTTCAATAGTATCATGAAT+TGG + tig0028755:36257-36276 None:intergenic 25.0%
! AGATTGACACTTCATATTAA+TGG + tig0028755:36624-36643 None:intergenic 25.0%
! CACATATATTTCCAGATTTA+AGG + tig0028755:36788-36807 None:intergenic 25.0%
!! AAAAGCATATGAAATTGGAA+TGG - tig0028755:36892-36911 Msa1439410:five_prime_UTR 25.0%
! AAAGCATATGAAATTGGAAT+GGG - tig0028755:36893-36912 Msa1439410:five_prime_UTR 25.0%
!! GTTTTTCTCATAGTGATTTT+TGG - tig0028755:37186-37205 Msa1439410:five_prime_UTR 25.0%
!!! TTTGAGTTTTGTGCTTATTA+TGG - tig0028755:37434-37453 Msa1439410:five_prime_UTR 25.0%
!!! TTGAGTTTTGTGCTTATTAT+GGG - tig0028755:37435-37454 Msa1439410:five_prime_UTR 25.0%
!!! GGTTTTTTTACACGTGATTT+TGG + tig0028755:33127-33146 None:intergenic 30.0%
!! GATTCTTGATGATCTTTTGT+GGG + tig0028755:33272-33291 None:intergenic 30.0%
!! AGATTCTTGATGATCTTTTG+TGG + tig0028755:33273-33292 None:intergenic 30.0%
AATCTTGAAAGATGCAGTTA+TGG - tig0028755:33571-33590 Msa1439410:CDS 30.0%
GTTGTGATCGTAATCTTAAA+GGG - tig0028755:33735-33754 Msa1439410:intron 30.0%
!! AAGTGATTTGCTAACATCAA+CGG + tig0028755:33771-33790 None:intergenic 30.0%
GCACATGTTAAAACAAGTAT+AGG - tig0028755:34469-34488 Msa1439410:CDS 30.0%
TGGCATTCTGTCTTAAAAAT+TGG - tig0028755:35125-35144 Msa1439410:intron 30.0%
AGTAAAGCTCATGTTAGAAA+TGG + tig0028755:35219-35238 None:intergenic 30.0%
GTTGGCATTGTAACAAAAAT+TGG - tig0028755:35321-35340 Msa1439410:intron 30.0%
! ATTCTTTCTAGTAATGCCAT+TGG + tig0028755:35769-35788 None:intergenic 30.0%
! GAATAAGTGATCTTCTAAGA+TGG - tig0028755:35785-35804 Msa1439410:intron 30.0%
TCCATGGTAAATTGAATGAA+GGG + tig0028755:35822-35841 None:intergenic 30.0%
TTCCATGGTAAATTGAATGA+AGG + tig0028755:35823-35842 None:intergenic 30.0%
TGAAAATCACTTTCTTCTTG+TGG + tig0028755:35918-35937 None:intergenic 30.0%
GACAAAGTTGTTGTAATCTT+TGG + tig0028755:35950-35969 None:intergenic 30.0%
TATGTGAAGTAATCAATAGC+TGG + tig0028755:36032-36051 None:intergenic 30.0%
GATGATAATCCTATTGTGTT+TGG - tig0028755:36077-36096 Msa1439410:intron 30.0%
TGAATTGTTCCAAACACAAT+AGG + tig0028755:36089-36108 None:intergenic 30.0%
AATTCCTATGGGATTTAAGT+TGG - tig0028755:36172-36191 Msa1439410:intron 30.0%
! TATGTCTACAGAAAGTACAT+TGG - tig0028755:36208-36227 Msa1439410:intron 30.0%
TCAGATGTTCATATATGTGT+CGG + tig0028755:36468-36487 None:intergenic 30.0%
!! TTTTTGTTGTGTAGGGAAAT+TGG - tig0028755:36862-36881 Msa1439410:five_prime_UTR 30.0%
GATTTGATAGACAACTCATA+AGG - tig0028755:37095-37114 Msa1439410:five_prime_UTR 30.0%
! ATTTTGTACAGAAAAGAGCT+TGG - tig0028755:37215-37234 Msa1439410:five_prime_UTR 30.0%
ATAAGGTCTGTTAATGAAGC+TGG - tig0028755:33662-33681 Msa1439410:CDS 35.0%
! GAAGATGCACATGATAATGA+TGG - tig0028755:33713-33732 Msa1439410:intron 35.0%
GGTTGTGATCGTAATCTTAA+AGG - tig0028755:33734-33753 Msa1439410:intron 35.0%
TAGAAACTTACAGAAGATGG+AGG + tig0028755:33794-33813 None:intergenic 35.0%
TTTGGTCTAATAATGTAGCG+TGG - tig0028755:33946-33965 Msa1439410:intron 35.0%
!! TTTCTATTTCTGGAAGGGAT+TGG + tig0028755:34509-34528 None:intergenic 35.0%
!! ATGGCTTTCTATTTCTGGAA+GGG + tig0028755:34514-34533 None:intergenic 35.0%
!! AGTTGATGGCTTTCTATTTC+TGG + tig0028755:34519-34538 None:intergenic 35.0%
! TTAAACTAGTTTGTGCCTCA+TGG - tig0028755:34594-34613 Msa1439410:CDS 35.0%
!! AACGTGTGTGTTTTATTGTG+AGG - tig0028755:34655-34674 Msa1439410:CDS 35.0%
GTGATGAGTATGAATTGAGT+TGG - tig0028755:34706-34725 Msa1439410:CDS 35.0%
GACATTGAGTCATATATCGT+TGG - tig0028755:34926-34945 Msa1439410:intron 35.0%
ACATTGTACTTCAACTAGGT+GGG + tig0028755:35102-35121 None:intergenic 35.0%
AACCACATTGTACTTCAACT+AGG + tig0028755:35106-35125 None:intergenic 35.0%
TAGTTGAAGTACAATGTGGT+TGG - tig0028755:35105-35124 Msa1439410:intron 35.0%
AACAACATGTTCGTACTCAT+TGG - tig0028755:35520-35539 Msa1439410:intron 35.0%
TCAAAAATCGAATTGCGTGA+AGG + tig0028755:35656-35675 None:intergenic 35.0%
!! ACGCAATTCGATTTTTGAAG+TGG - tig0028755:35658-35677 Msa1439410:intron 35.0%
AATCACTGCAATATGTCCAA+TGG - tig0028755:35750-35769 Msa1439410:intron 35.0%
ACCCTTCATTCAATTTACCA+TGG - tig0028755:35818-35837 Msa1439410:intron 35.0%
! TGGACTTGATTCTGAGAAAT+TGG + tig0028755:35859-35878 None:intergenic 35.0%
!!! GTTCTGGTTTTTTTGTAGTG+TGG + tig0028755:35887-35906 None:intergenic 35.0%
! CTTTCTTCTTGTGGTGTTAA+TGG + tig0028755:35909-35928 None:intergenic 35.0%
ACACAAGCTGCTGAAAAAAT+TGG - tig0028755:36149-36168 Msa1439410:intron 35.0%
CTAACCAACTTAAATCCCAT+AGG + tig0028755:36179-36198 None:intergenic 35.0%
!! ATGGGATTTAAGTTGGTTAG+AGG - tig0028755:36179-36198 Msa1439410:intron 35.0%
!! TTGCTTGAGAAGTTTGCTAA+TGG - tig0028755:36311-36330 Msa1439410:intron 35.0%
!! GAGAAGTTTGCTAATGGAAA+AGG - tig0028755:36317-36336 Msa1439410:intron 35.0%
AAGTCTGATTCCGATATATG+TGG + tig0028755:36739-36758 None:intergenic 35.0%
! GCAGCAAAAGCATATGAAAT+TGG - tig0028755:36887-36906 Msa1439410:five_prime_UTR 35.0%
! GTTGTGCAAATTCAAGTTTG+TGG + tig0028755:36928-36947 None:intergenic 35.0%
CTTGAATTTGCACAACTATG+TGG - tig0028755:36932-36951 Msa1439410:five_prime_UTR 35.0%
ATGTCAGATGCACTTTCATT+TGG - tig0028755:36956-36975 Msa1439410:five_prime_UTR 35.0%
GTTAGCAAGTATATGCCATT+TGG - tig0028755:37001-37020 Msa1439410:five_prime_UTR 35.0%
TTTCTTCAGCTCTTCTCAAT+AGG + tig0028755:37051-37070 None:intergenic 35.0%
AGAAGAGCTGAAGAAAATAG+AGG - tig0028755:37055-37074 Msa1439410:five_prime_UTR 35.0%
! TGAAGAAAATAGAGGCCTTT+TGG - tig0028755:37063-37082 Msa1439410:five_prime_UTR 35.0%
GAAACTCTCGACAACACTTT+AGG + tig0028755:33148-33167 None:intergenic 40.0%
! TTTTTGAATGGCAACTCGTG+TGG - tig0028755:33245-33264 Msa1439410:three_prime_UTR 40.0%
AGGTTGTTCTCTTTCGAGAA+GGG + tig0028755:33360-33379 None:intergenic 40.0%
AAGGTTGTTCTCTTTCGAGA+AGG + tig0028755:33361-33380 None:intergenic 40.0%
TCGTTTAGGTCGAGATAAGA+CGG + tig0028755:33419-33438 None:intergenic 40.0%
! TTGCTGCTGGAAAGAGTATA+AGG - tig0028755:33645-33664 Msa1439410:CDS 40.0%
TTACGTGGCATGCTTGTTTA+TGG - tig0028755:33686-33705 Msa1439410:CDS 40.0%
!! GATGGCTTTCTATTTCTGGA+AGG + tig0028755:34515-34534 None:intergenic 40.0%
!!! TGCCTCATGGTTTTAGTTGT+GGG - tig0028755:34607-34626 Msa1439410:CDS 40.0%
AACCCACAACTAAAACCATG+AGG + tig0028755:34612-34631 None:intergenic 40.0%
!!! ATGGTTTTAGTTGTGGGTTG+TGG - tig0028755:34613-34632 Msa1439410:CDS 40.0%
!!! TGGTTTTAGTTGTGGGTTGT+GGG - tig0028755:34614-34633 Msa1439410:CDS 40.0%
AGAAGTAGCATGACTTGAAC+TGG + tig0028755:34785-34804 None:intergenic 40.0%
TCTTGGAATCATGTGACAGA+CGG - tig0028755:34839-34858 Msa1439410:five_prime_UTR 40.0%
ACATTGTCCTTAAGCTAGGT+GGG + tig0028755:34897-34916 None:intergenic 40.0%
AACCACATTGTCCTTAAGCT+AGG + tig0028755:34901-34920 None:intergenic 40.0%
TAGCTTAAGGACAATGTGGT+TGG - tig0028755:34900-34919 Msa1439410:intron 40.0%
ATTGTACTTCAACTAGGTGG+GGG + tig0028755:35100-35119 None:intergenic 40.0%
CATTGTACTTCAACTAGGTG+GGG + tig0028755:35101-35120 None:intergenic 40.0%
CACATTGTACTTCAACTAGG+TGG + tig0028755:35103-35122 None:intergenic 40.0%
CACCTAGTTGAAGTACAATG+TGG - tig0028755:35101-35120 Msa1439410:intron 40.0%
ACATTGTACATCAGCTAGGT+GGG + tig0028755:35300-35319 None:intergenic 40.0%
AACCACATTGTACATCAGCT+AGG + tig0028755:35304-35323 None:intergenic 40.0%
TAGCTGATGTACAATGTGGT+TGG - tig0028755:35303-35322 Msa1439410:intron 40.0%
GGGTTCATTAGTACGTAGTT+TGG - tig0028755:35386-35405 Msa1439410:intron 40.0%
TGTTCGTACTCATTGGATCA+AGG - tig0028755:35527-35546 Msa1439410:intron 40.0%
AAAATCGAATTGCGTGAAGG+TGG + tig0028755:35653-35672 None:intergenic 40.0%
! TCTTGTGGTGTTAATGGTTC+TGG + tig0028755:35903-35922 None:intergenic 40.0%
TGAAGCAAACATGCCACTAT+TGG - tig0028755:35980-35999 Msa1439410:intron 40.0%
TGTTCAGCATCAACCAATAG+TGG + tig0028755:35996-36015 None:intergenic 40.0%
!! AGTACATTGGCTGAGTCTTT+TGG - tig0028755:36221-36240 Msa1439410:intron 40.0%
GCTACACACAACATTGAATC+AGG - tig0028755:36353-36372 Msa1439410:intron 40.0%
!! TCATTTGGTTTGAGCAATGC+AGG - tig0028755:36971-36990 Msa1439410:five_prime_UTR 40.0%
!! CATTTGGTTTGAGCAATGCA+GGG - tig0028755:36972-36991 Msa1439410:five_prime_UTR 40.0%
TAAGGCATAACCATCTCAAC+TGG + tig0028755:37028-37047 None:intergenic 40.0%
TCAGCTCTTCTCAATAGGTA+AGG + tig0028755:37046-37065 None:intergenic 40.0%
TGTACAGAAAAGAGCTTGGA+AGG - tig0028755:37219-37238 Msa1439410:five_prime_UTR 40.0%
!! TCACTTTTATACTGTAGGGG+CGG + tig0028755:37467-37486 None:intergenic 40.0%
!!! GGCTTGTGGTGGTTTTGAAA+CGG + tig0028755:33296-33315 None:intergenic 45.0%
! AGGAGTTTAATGGCTTGTGG+TGG + tig0028755:33307-33326 None:intergenic 45.0%
GAAGGATGGGAGGAGTTTAA+TGG + tig0028755:33317-33336 None:intergenic 45.0%
TTGTTCTCTTTCGAGAAGGG+AGG + tig0028755:33357-33376 None:intergenic 45.0%
TAAGACGGTTGTTGGTGTGA+CGG + tig0028755:33404-33423 None:intergenic 45.0%
ATAGTCTCTCAGCGTCGTTT+AGG + tig0028755:33433-33452 None:intergenic 45.0%
GATCTTATCAACGTGGACGT+TGG + tig0028755:33467-33486 None:intergenic 45.0%
TATAGCCGTTGCATGCAGAA+CGG + tig0028755:33495-33514 None:intergenic 45.0%
TGTCGTGGACGTTGGAATAT+GGG - tig0028755:33520-33539 Msa1439410:CDS 45.0%
! GTTAATGAAGCTGGCTTACG+TGG - tig0028755:33671-33690 Msa1439410:CDS 45.0%
TGGACTCACCCCAATAGTTA+AGG - tig0028755:34409-34428 Msa1439410:CDS 45.0%
!!! GTGCCTCATGGTTTTAGTTG+TGG - tig0028755:34606-34625 Msa1439410:CDS 45.0%
!!! GGTTTTAGTTGTGGGTTGTG+GGG - tig0028755:34615-34634 Msa1439410:CDS 45.0%
TATTGGTCCCACCTAGCTTA+AGG - tig0028755:34887-34906 Msa1439410:intron 45.0%
CACATTGTCCTTAAGCTAGG+TGG + tig0028755:34898-34917 None:intergenic 45.0%
CACCTAGCTTAAGGACAATG+TGG - tig0028755:34896-34915 Msa1439410:intron 45.0%
TTGTACTTCAACTAGGTGGG+GGG + tig0028755:35099-35118 None:intergenic 45.0%
CACATTGTACATCAGCTAGG+TGG + tig0028755:35301-35320 None:intergenic 45.0%
CACCTAGCTGATGTACAATG+TGG - tig0028755:35299-35318 Msa1439410:intron 45.0%
GTCAGCACATTCATGACAGT+TGG + tig0028755:35610-35629 None:intergenic 45.0%
!! GGTAGAGAGTCTTGTTTCCA+TGG + tig0028755:35838-35857 None:intergenic 45.0%
! TGGCTGAGTCTTTTGGATCT+AGG - tig0028755:36228-36247 Msa1439410:intron 45.0%
! TGTTTTGTGTCGTCGCATGT+TGG - tig0028755:36533-36552 Msa1439410:intron 45.0%
GTGTTTGTGTTGTCGCATGT+CGG - tig0028755:36590-36609 Msa1439410:intron 45.0%
TGACACTGACACGTAGACAA+TGG - tig0028755:36678-36697 Msa1439410:intron 45.0%
AACCAGACACGCCTTAAATC+TGG - tig0028755:36774-36793 Msa1439410:intron 45.0%
! TTCCAGATTTAAGGCGTGTC+TGG + tig0028755:36779-36798 None:intergenic 45.0%
ACCATCTCAACTGGTCCAAA+TGG + tig0028755:37019-37038 None:intergenic 45.0%
CAAATCCAGAAGCAGCCAAA+AGG + tig0028755:37081-37100 None:intergenic 45.0%
ACAGAAAAGAGCTTGGAAGG+AGG - tig0028755:37222-37241 Msa1439410:five_prime_UTR 45.0%
!! AATAATAATAATAATATAAG+AGG - tig0028755:34351-34370 Msa1439410:CDS 5.0%
!! TAAAAAATATAAAATTGAAA+AGG + tig0028755:36416-36435 None:intergenic 5.0%
GGGAGGAGTTTAATGGCTTG+TGG + tig0028755:33310-33329 None:intergenic 50.0%
GGTCGAGATAAGACGGTTGT+TGG + tig0028755:33412-33431 None:intergenic 50.0%
GGCTGAGGATCTTATCAACG+TGG + tig0028755:33474-33493 None:intergenic 50.0%
GTTCTGCATGCAACGGCTAT+AGG - tig0028755:33494-33513 Msa1439410:CDS 50.0%
CATATTCCAACGTCCACGAC+AGG + tig0028755:33521-33540 None:intergenic 50.0%
CTGTCGTGGACGTTGGAATA+TGG - tig0028755:33519-33538 Msa1439410:CDS 50.0%
GGAGAAGATGCTATTGCTGC+TGG - tig0028755:33632-33651 Msa1439410:CDS 50.0%
TACATCAGCTAGGTGGGAGT+GGG + tig0028755:35294-35313 None:intergenic 50.0%
CGTGTCTGGTTGATCGATGT+GGG + tig0028755:36765-36784 None:intergenic 50.0%
GCCATTTGGACCAGTTGAGA+TGG - tig0028755:37015-37034 Msa1439410:five_prime_UTR 50.0%
TCTTTCGAGAAGGGAGGCGA+CGG + tig0028755:33351-33370 None:intergenic 55.0%
TGTTGGTGTGACGGTTGTGG+TGG + tig0028755:33395-33414 None:intergenic 55.0%
GGTTGTTGGTGTGACGGTTG+TGG + tig0028755:33398-33417 None:intergenic 55.0%
CTCAGCCGTTCTGCATGCAA+CGG - tig0028755:33487-33506 Msa1439410:CDS 55.0%
AACGGCTATAGGACCTGTCG+TGG - tig0028755:33505-33524 Msa1439410:CDS 55.0%
ATAGGACCTGTCGTGGACGT+TGG - tig0028755:33512-33531 Msa1439410:CDS 55.0%
GTACATCAGCTAGGTGGGAG+TGG + tig0028755:35295-35314 None:intergenic 55.0%
GCGTGTCTGGTTGATCGATG+TGG + tig0028755:36766-36785 None:intergenic 55.0%
!! AGAGGCCTTTTGGCTGCTTC+TGG - tig0028755:37073-37092 Msa1439410:five_prime_UTR 55.0%
!! TGTGGTGGTGGTGGTGGAGA+AGG + tig0028755:33380-33399 None:intergenic 60.0%
!! TGTGACGGTTGTGGTGGTGG+TGG + tig0028755:33389-33408 None:intergenic 60.0%
TGGTGTGACGGTTGTGGTGG+TGG + tig0028755:33392-33411 None:intergenic 60.0%
CGTTGCATGCAGAACGGCTG+AGG + tig0028755:33489-33508 None:intergenic 60.0%
AGCGGTGGCTGAAGGATGGG+AGG + tig0028755:33327-33346 None:intergenic 65.0%
GGCAGCGGTGGCTGAAGGAT+GGG + tig0028755:33330-33349 None:intergenic 65.0%
!! GACGGTTGTGGTGGTGGTGG+TGG + tig0028755:33386-33405 None:intergenic 65.0%
CGGCAGCGGTGGCTGAAGGA+TGG + tig0028755:33331-33350 None:intergenic 70.0%
GAGAAGGGAGGCGACGGCAG+CGG + tig0028755:33345-33364 None:intergenic 70.0%
GCGACGGCAGCGGTGGCTGA+AGG + tig0028755:33335-33354 None:intergenic 75.0%
AAGGGAGGCGACGGCAGCGG+TGG + tig0028755:33342-33361 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
tig0028755 gene 33110 37487 33110 ID=Msa1439410;Name=Msa1439410
tig0028755 mRNA 33110 37487 33110 ID=Msa1439410-mRNA-1;Parent=Msa1439410;Name=Msa1439410-mRNA-1;_AED=0.00;_eAED=0.00;_QI=767|1|1|1|0.66|0.5|4|222|283
tig0028755 exon 33110 33371 33110 ID=Msa1439410-mRNA-1:exon:11765;Parent=Msa1439410-mRNA-1
tig0028755 exon 33481 33721 33481 ID=Msa1439410-mRNA-1:exon:11764;Parent=Msa1439410-mRNA-1
tig0028755 exon 34223 34867 34223 ID=Msa1439410-mRNA-1:exon:11763;Parent=Msa1439410-mRNA-1
tig0028755 exon 36795 37487 36795 ID=Msa1439410-mRNA-1:exon:11762;Parent=Msa1439410-mRNA-1
tig0028755 five_prime_UTR 36795 37487 36795 ID=Msa1439410-mRNA-1:five_prime_utr;Parent=Msa1439410-mRNA-1
tig0028755 five_prime_UTR 34794 34867 34794 ID=Msa1439410-mRNA-1:five_prime_utr;Parent=Msa1439410-mRNA-1
tig0028755 CDS 34223 34793 34223 ID=Msa1439410-mRNA-1:cds;Parent=Msa1439410-mRNA-1
tig0028755 CDS 33481 33721 33481 ID=Msa1439410-mRNA-1:cds;Parent=Msa1439410-mRNA-1
tig0028755 CDS 33332 33371 33332 ID=Msa1439410-mRNA-1:cds;Parent=Msa1439410-mRNA-1
tig0028755 three_prime_UTR 33110 33331 33110 ID=Msa1439410-mRNA-1:three_prime_utr;Parent=Msa1439410-mRNA-1
Gene Sequence

>Msa1439410

ATGGCAACAAAAAGACCCTTCATTCAATTTACCATGGAAACAAGACTCTCTACCAATTTCTCAGAATCAAGTCCATTATACCACACTACAAAAAAACCAGAACCATTAACACCACAAGAAGAAAGTGATTTTCAACTAGCAAACCAAAGATTACAACAACTTTGTCAAAAATGTGTTGAAGCAAACATGCCACTATTGGTTGATGCTGAACACACTACTGTTCAACCAGCTATTGATTACTTCACATACTCTTCAGCAATTATGCATAATAAAGATGATAATCCTATTGTGTTTGGAACAATTCAAACTTATTTGAAAGATGCTAAGGAGAGATTGTTTCTTGCAACACAAGCTGCTGAAAAAATTGGAATTCCTATGGGATTTAAGTTGGTTAGAGGTGCTTATATGTCTACAGAAAGTACATTGGCTGAGTCTTTTGGATCTAGGTCACCAATTCATGATACTATTGAAGATACACATAATTGCTTCAATGATTGTTCATCTTATTTGCTTGAGAAGTTTGCTAATGGAAAAGGATCTGTTGTTCTTGCTACACACAACATTGAATCAGGGAAATTGGCTGCAGCAAAAGCATATGAAATTGGAATGGGAAAAGTGAACCACAAACTTGAATTTGCACAACTATGTGGAATGTCAGATGCACTTTCATTTGGTTTGAGCAATGCAGGGTTTAGAGTTAGCAAGTATATGCCATTTGGACCAGTTGAGATGGTTATGCCTTACCTATTGAGAAGAGCTGAAGAAAATAGAGGCCTTTTGGCTGCTTCTGGATTTGATAGACAACTCATAAGAAAAGAGCTTGGAAGGAGGCTTAAAGCTGCAATATTTTAA
Protein sequence

>Msa1439410

MATKRPFIQFTMETRLSTNFSESSPLYHTTKKPEPLTPQEESDFQLANQRLQQLCQKCVEANMPLLVDAEHTTVQPAIDYFTYSSAIMHNKDDNPIVFGTIQTYLKDAKERLFLATQAAEKIGIPMGFKLVRGAYMSTESTLAESFGSRSPIHDTIEDTHNCFNDCSSYLLEKFANGKGSVVLATHNIESGKLAAAKAYEIGMGKVNHKLEFAQLCGMSDALSFGLSNAGFRVSKYMPFGPVEMVMPYLLRRAEENRGLLAASGFDRQLIRKELGRRLKAAIF*