AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0608700

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0608700 MtrunA17_Chr4g0041011 97.534 365 5 1 1 361 1 365 0.0 748
Msa0608700 MtrunA17_Chr2g0325481 75.783 351 77 1 17 359 16 366 0.0 566
Msa0608700 MtrunA17_Chr2g0283581 59.322 354 139 4 10 360 8 359 5.92e-154 437
Msa0608700 MtrunA17_Chr4g0045951 59.748 318 127 1 43 359 45 362 1.60e-142 408
Msa0608700 MtrunA17_Chr4g0045941 58.255 321 133 1 40 359 30 350 7.51e-140 401
Msa0608700 MtrunA17_Chr3g0097891 55.937 320 138 2 43 359 31 350 3.08e-135 389
Msa0608700 MtrunA17_Chr2g0314801 56.289 318 135 3 43 356 33 350 9.60e-132 380
Msa0608700 MtrunA17_Chr5g0413851 51.532 359 159 4 4 359 13 359 1.42e-130 378
Msa0608700 MtrunA17_Chr3g0085071 51.351 333 155 2 30 361 39 365 1.01e-128 373
Msa0608700 MtrunA17_Chr3g0091031 50.000 332 159 2 31 361 25 350 8.15e-125 363
Msa0608700 MtrunA17_Chr3g0085081 49.699 332 160 2 31 361 25 350 9.58e-124 360
Msa0608700 MtrunA17_Chr5g0430371 46.499 357 166 5 28 359 43 399 2.76e-117 345
Msa0608700 MtrunA17_Chr3g0105301 49.254 335 153 4 42 359 42 376 1.31e-116 343
Msa0608700 MtrunA17_Chr2g0314791 47.401 327 152 6 43 359 29 345 3.09e-105 313
Msa0608700 MtrunA17_Chr1g0175671 38.859 368 198 6 16 359 145 509 1.46e-88 276
Msa0608700 MtrunA17_Chr1g0171171 39.881 336 180 7 38 359 48 375 5.99e-88 270
Msa0608700 MtrunA17_Chr7g0258851 40.643 342 176 7 43 359 248 587 4.65e-86 271
Msa0608700 MtrunA17_Chr1g0210771 36.842 361 194 10 23 359 32 382 1.02e-83 259
Msa0608700 MtrunA17_Chr4g0068111 37.901 343 186 8 42 359 113 453 4.33e-80 252
Msa0608700 MtrunA17_Chr5g0437291 38.329 347 184 9 43 359 165 511 5.13e-80 253
Msa0608700 MtrunA17_Chr4g0071391 33.242 364 219 7 18 359 85 446 8.39e-80 251
Msa0608700 MtrunA17_Chr3g0113671 38.372 344 185 9 43 359 106 449 1.67e-79 250
Msa0608700 MtrunA17_Chr1g0213281 36.782 348 192 7 38 359 170 515 1.96e-79 252
Msa0608700 MtrunA17_Chr7g0269991 36.905 336 188 8 43 359 137 467 3.36e-78 248
Msa0608700 MtrunA17_Chr3g0114101 37.318 343 188 9 43 359 89 430 6.12e-76 241
Msa0608700 MtrunA17_Chr5g0437601 36.182 351 199 7 34 359 74 424 6.62e-75 238
Msa0608700 MtrunA17_Chr4g0006991 34.795 342 198 7 43 359 86 427 6.23e-74 235
Msa0608700 MtrunA17_Chr8g0360521 36.000 350 188 10 39 359 67 409 8.48e-74 234
Msa0608700 MtrunA17_Chr5g0413271 32.665 349 218 4 27 359 129 476 1.25e-72 233
Msa0608700 MtrunA17_Chr8g0368461 33.705 359 202 7 36 359 171 528 1.85e-70 229
Msa0608700 MtrunA17_Chr4g0005491 31.854 383 231 9 8 361 28 409 3.98e-69 222
Msa0608700 MtrunA17_Chr3g0126021 37.609 343 189 8 37 359 122 459 2.17e-68 222
Msa0608700 MtrunA17_Chr7g0259711 35.756 344 192 9 43 359 91 432 2.42e-68 221
Msa0608700 MtrunA17_Chr7g0243301 31.620 389 232 11 1 359 25 409 6.42e-68 219
Msa0608700 MtrunA17_Chr1g0209921 48.035 229 112 6 40 264 41 266 6.92e-68 214
Msa0608700 MtrunA17_Chr2g0287901 34.694 343 198 7 40 357 94 435 1.66e-67 219
Msa0608700 MtrunA17_Chr4g0005481 34.795 342 197 8 42 359 107 446 8.11e-67 217
Msa0608700 MtrunA17_Chr7g0242701 35.965 342 194 7 43 359 86 427 1.64e-66 216
Msa0608700 MtrunA17_Chr8g0384151 32.344 337 198 9 43 359 90 416 1.60e-57 192
Msa0608700 MtrunA17_Chr1g0178081 30.831 373 171 12 12 359 31 341 5.91e-47 163
Msa0608700 MtrunA17_Chr4g0049421 29.395 347 205 13 43 355 48 388 3.38e-38 140
Msa0608700 MtrunA17_Chr6g0455901 30.352 369 218 12 27 360 53 417 1.91e-37 139
Msa0608700 MtrunA17_Chr6g0476041 27.869 366 209 11 37 359 161 514 1.45e-36 138
Msa0608700 MtrunA17_Chr1g0188191 29.577 355 197 14 43 355 234 577 4.42e-35 135
Msa0608700 MtrunA17_Chr8g0384421 29.752 363 216 15 28 359 118 472 1.03e-34 132
Msa0608700 MtrunA17_Chr5g0414051 30.899 356 197 16 43 359 75 420 6.79e-34 130
Msa0608700 MtrunA17_Chr6g0456691 27.500 400 239 13 1 359 30 419 9.88e-33 126
Msa0608700 MtrunA17_Chr5g0414061 29.143 350 210 13 43 359 94 438 5.95e-32 124
Msa0608700 MtrunA17_Chr5g0414091 29.429 350 213 12 42 359 84 431 9.23e-32 124
Msa0608700 MtrunA17_Chr7g0226991 27.851 377 231 13 17 359 33 402 1.47e-30 120
Msa0608700 MtrunA17_Chr6g0456681 26.781 351 217 10 43 359 64 408 1.88e-30 120
Msa0608700 MtrunA17_Chr8g0391481 27.920 351 218 10 43 361 27 374 1.20e-29 117
Msa0608700 MtrunA17_Chr5g0414041 28.329 353 213 11 42 359 86 433 2.27e-29 117
Msa0608700 MtrunA17_Chr4g0070341 26.882 372 211 14 27 359 122 471 2.52e-29 117
Msa0608700 MtrunA17_Chr2g0283851 27.350 351 223 9 36 355 47 396 3.89e-29 116
Msa0608700 MtrunA17_Chr8g0347321 26.972 393 220 16 15 359 66 439 7.65e-29 116
Msa0608700 MtrunA17_Chr3g0085101 42.982 114 64 1 227 339 1 114 1.32e-26 102
Msa0608700 MtrunA17_Chr3g0140371 28.814 354 204 15 43 359 18 360 2.22e-26 108
Msa0608700 MtrunA17_Chr6g0451321 27.066 351 215 10 42 359 115 457 8.72e-26 107
Msa0608700 MtrunA17_Chr8g0387921 24.438 356 218 9 40 355 71 415 9.52e-24 101
Msa0608700 MtrunA17_Chr7g0221291 40.196 102 61 0 30 131 66 167 2.34e-19 84.3
Msa0608700 MtrunA17_Chr7g0221271 32.857 140 94 0 30 169 66 205 3.58e-19 84.7
Msa0608700 MtrunA17_Chr1g0209931 44.944 89 40 3 275 359 9 92 1.26e-16 74.7
Msa0608700 MtrunA17_Chr5g0429211 47.368 76 40 0 97 172 194 269 1.51e-15 76.3
Msa0608700 MtrunA17_Chr2g0287911 45.763 59 25 1 40 98 58 109 2.26e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0608700 AT2G42570.1 63.855 332 119 1 29 359 33 364 1.80e-166 469
Msa0608700 AT2G31110.2 58.659 358 144 3 6 360 7 363 3.00e-163 461
Msa0608700 AT1G29050.1 61.491 322 123 1 40 360 58 379 2.01e-155 442
Msa0608700 AT2G34070.1 61.250 320 122 2 43 360 65 384 1.01e-149 427
Msa0608700 AT3G14850.2 57.009 321 134 3 40 356 31 351 1.47e-133 385
Msa0608700 AT1G78710.1 50.440 341 162 5 23 359 19 356 1.27e-124 363
Msa0608700 AT2G30900.1 50.769 325 154 4 43 361 43 367 3.01e-120 352
Msa0608700 AT2G34070.2 61.200 250 95 2 113 360 1 250 1.91e-113 330
Msa0608700 AT5G58600.1 48.214 336 156 5 42 359 65 400 8.46e-110 327
Msa0608700 AT2G31110.3 56.693 254 107 2 6 257 7 259 2.19e-106 313
Msa0608700 AT2G30010.1 42.328 378 194 5 6 359 19 396 1.93e-103 310
Msa0608700 AT2G30010.2 42.328 378 194 5 6 359 62 439 4.41e-103 311
Msa0608700 AT2G31110.1 63.462 208 75 1 154 360 8 215 3.57e-102 300
Msa0608700 AT3G14850.1 56.048 248 105 3 113 356 1 248 3.44e-96 286
Msa0608700 AT1G78710.2 41.944 360 141 7 4 359 1 296 3.05e-93 281
Msa0608700 AT5G06700.1 41.096 365 190 6 19 359 231 594 2.13e-90 283
Msa0608700 AT3G54260.1 39.940 333 175 6 43 359 51 374 8.12e-86 265
Msa0608700 AT3G12060.1 39.474 342 182 5 42 359 195 535 1.65e-85 269
Msa0608700 AT2G30900.2 52.511 219 102 2 43 259 43 261 9.61e-84 256
Msa0608700 AT3G62390.1 38.166 338 188 6 43 361 138 473 3.53e-81 256
Msa0608700 AT5G58600.2 50.667 225 109 2 42 264 65 289 5.52e-79 244
Msa0608700 AT5G49340.1 36.544 353 181 7 41 359 98 441 6.18e-79 249
Msa0608700 AT3G55990.1 35.792 366 206 9 21 359 120 483 5.02e-78 248
Msa0608700 AT5G20590.1 35.882 340 194 7 43 359 141 479 1.24e-77 247
Msa0608700 AT1G73140.1 37.209 344 186 10 42 359 66 405 1.93e-75 239
Msa0608700 AT2G40160.2 35.714 350 193 9 43 361 77 425 2.50e-75 239
Msa0608700 AT2G40160.1 35.756 344 189 9 43 355 77 419 8.03e-75 238
Msa0608700 AT1G60790.1 36.047 344 192 7 43 359 190 532 2.39e-74 240
Msa0608700 AT2G40150.1 34.571 350 196 8 43 359 71 420 6.61e-74 235
Msa0608700 AT3G11030.1 35.694 353 199 9 34 359 97 448 2.51e-72 232
Msa0608700 AT5G01620.2 34.571 350 202 8 35 359 102 449 8.61e-72 231
Msa0608700 AT5G01620.1 34.571 350 202 8 35 359 102 449 8.61e-72 231
Msa0608700 AT5G01620.3 34.571 350 202 8 35 359 110 457 1.20e-71 230
Msa0608700 AT2G40320.1 34.521 365 214 8 20 359 58 422 2.60e-71 229
Msa0608700 AT5G01360.2 34.985 343 197 8 43 359 36 378 1.44e-70 225
Msa0608700 AT5G01360.1 34.985 343 197 8 43 359 92 434 5.62e-70 225
Msa0608700 AT2G38320.1 32.216 388 233 9 1 359 18 404 7.49e-70 224
Msa0608700 AT5G19160.1 34.109 387 203 10 19 357 60 442 1.14e-69 226
Msa0608700 AT3G06080.2 35.380 342 193 8 43 357 103 443 7.82e-68 221
Msa0608700 AT1G48880.1 35.310 371 215 9 1 355 74 435 2.64e-66 216
Msa0608700 AT5G06230.2 36.000 350 193 10 39 359 17 364 7.95e-66 213
Msa0608700 AT5G06230.1 36.000 350 193 10 39 359 58 405 2.28e-65 213
Msa0608700 AT3G11570.1 36.223 323 183 6 59 359 98 419 2.27e-64 211
Msa0608700 AT2G38320.2 31.761 318 189 7 70 359 4 321 3.88e-56 186
Msa0608700 AT3G62390.2 36.709 237 138 3 43 267 138 374 6.45e-55 186
Msa0608700 AT2G40160.3 37.179 234 135 5 43 264 77 310 2.97e-52 176
Msa0608700 AT5G20590.2 35.294 238 140 5 43 266 141 378 2.90e-51 175
Msa0608700 AT5G19160.2 33.935 277 154 5 19 266 60 336 3.81e-49 169
Msa0608700 AT3G06080.1 35.714 238 139 4 43 266 103 340 1.80e-46 162
Msa0608700 AT5G06230.3 35.081 248 141 5 39 266 58 305 2.05e-44 155
Msa0608700 AT2G14530.1 29.973 367 215 10 28 359 47 406 5.73e-42 151
Msa0608700 AT5G64470.3 29.799 349 212 12 36 355 46 390 6.31e-40 145
Msa0608700 AT5G64470.2 29.775 356 209 14 36 355 46 396 4.56e-38 141
Msa0608700 AT1G70230.1 29.412 340 210 10 43 359 79 411 5.50e-38 141
Msa0608700 AT5G51640.1 27.249 389 227 12 16 359 111 488 3.00e-36 137
Msa0608700 AT4G25360.1 27.632 380 222 13 20 355 150 520 9.19e-36 137
Msa0608700 AT4G25360.2 27.632 380 222 13 20 355 150 520 9.19e-36 137
Msa0608700 AT5G64020.1 29.444 360 205 15 42 359 56 408 3.94e-34 130
Msa0608700 AT2G37720.1 29.640 361 191 15 42 359 142 482 2.06e-32 127
Msa0608700 AT5G15900.1 25.862 406 242 14 1 359 16 409 3.24e-32 125
Msa0608700 AT5G15900.2 25.862 406 242 14 1 359 35 428 4.49e-32 125
Msa0608700 AT3G28150.1 27.577 388 244 13 5 361 31 412 2.49e-31 123
Msa0608700 AT1G01430.1 25.532 376 236 11 22 359 78 447 3.52e-30 120
Msa0608700 AT5G20680.6 27.855 359 198 15 43 359 118 457 7.05e-30 119
Msa0608700 AT5G20680.2 27.577 359 199 14 43 359 194 533 1.16e-29 119
Msa0608700 AT5G20680.4 27.577 359 199 14 43 359 212 551 1.17e-29 120
Msa0608700 AT5G20680.1 27.577 359 199 14 43 359 212 551 1.17e-29 120
Msa0608700 AT5G20680.3 27.577 359 199 14 43 359 212 551 1.17e-29 120
Msa0608700 AT5G20680.5 27.577 359 199 14 43 359 212 551 1.17e-29 120
Msa0608700 AT3G02440.2 24.928 349 223 10 43 359 135 476 7.13e-29 117
Msa0608700 AT3G02440.1 24.928 349 223 10 43 359 127 468 7.25e-29 117
Msa0608700 AT5G15890.1 26.724 348 204 12 43 355 187 518 6.16e-27 112
Msa0608700 AT5G64470.4 29.213 267 164 10 36 281 46 308 2.78e-24 101
Msa0608700 AT5G64470.1 29.213 267 164 10 36 281 46 308 4.19e-24 101
Msa0608700 AT4G01080.1 25.143 350 217 12 40 355 88 426 8.45e-24 102
Msa0608700 AT4G11090.1 24.866 374 223 13 30 359 66 425 1.49e-23 101
Msa0608700 AT4G23790.1 24.929 353 225 11 43 359 79 427 1.59e-21 95.5

Find 98 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCAAATTGAAAACATATT+TGG 0.172908 4_2:+55898357 None:intergenic
CTGAATGTGCTTCTAGAATT+TGG 0.184720 4_2:+55901019 None:intergenic
AACATATTTGGTACAATTAA+AGG 0.190331 4_2:+55898369 None:intergenic
CAAACCAAAGTCTTCTTCTT+AGG 0.227258 4_2:-55899004 Msa0608700:CDS
ATGGCATCCACCTATATTTC+TGG 0.239401 4_2:+55901255 None:intergenic
AAATATTTAATTTGATGTTT+TGG 0.255647 4_2:-55898469 Msa0608700:three_prime_UTR
TGTAGCCACTGGTGTCTTCC+TGG 0.277598 4_2:-55898629 Msa0608700:CDS
AGTGAGAAAGTGCCATATTT+TGG 0.279871 4_2:-55898809 Msa0608700:CDS
TTCTTGTAATTGGGCAATAA+AGG 0.292541 4_2:-55898446 Msa0608700:three_prime_UTR
CTTGGCATGTTACAAGAAAA+TGG 0.318130 4_2:+55901236 None:intergenic
AGAAGCTGATTCCAAGTATC+AGG 0.322948 4_2:+55898602 None:intergenic
CAAGCACTGAAACCATTTCT+TGG 0.325936 4_2:+55901510 None:intergenic
GGGTATTTCAAACCAAAATA+TGG 0.332479 4_2:+55898797 None:intergenic
GACAAATTAATTTGTTTGTA+AGG 0.347307 4_2:-55898535 Msa0608700:three_prime_UTR
ATTGTAAATGTTCATTGTTT+AGG 0.354264 4_2:-55898402 Msa0608700:three_prime_UTR
TGACAGATGGGATTATATTC+AGG 0.367092 4_2:-55899125 Msa0608700:intron
TTCCTGGACTTCCTGATACT+TGG 0.369841 4_2:-55898613 Msa0608700:CDS
TCACTCATGCATGACTTTGT+TGG 0.371357 4_2:+55898827 None:intergenic
TAGTATATCTTGGAAGGATT+TGG 0.383203 4_2:-55898335 Msa0608700:three_prime_UTR
ATAAGCTTTACCAGAAATAT+AGG 0.384304 4_2:-55901265 Msa0608700:CDS
TCAATTTGAATAGTATATCT+TGG 0.396256 4_2:-55898345 Msa0608700:three_prime_UTR
GTCACATCTAAGAAATAAAC+TGG 0.411760 4_2:+55898716 None:intergenic
AAGCACTGAAACCATTTCTT+GGG 0.414434 4_2:+55901511 None:intergenic
AAGAAGACTTTGGTTTGTGC+TGG 0.422122 4_2:+55899010 None:intergenic
GGGAAGAAGATTATGTTTGT+TGG 0.424391 4_2:-55901100 Msa0608700:CDS
CAAGCCATTGGTGTCCCTCC+AGG 0.427641 4_2:+55898776 None:intergenic
TTTGAAGTCAGTAACATTGC+AGG 0.428295 4_2:-55901404 Msa0608700:CDS
AACTTTCTGAAGGGTAATAG+AGG 0.429506 4_2:-55901121 Msa0608700:CDS
TCTATACCGCACAGCATACT+TGG 0.433438 4_2:-55899371 Msa0608700:CDS
TGAAGGTTATAGTGGTGTTA+TGG 0.434673 4_2:-55898660 Msa0608700:CDS
GTAACTTGTTCCGTGGCAAA+TGG 0.439848 4_2:-55901376 Msa0608700:CDS
TATCTCTTCAGTCACATTTG+AGG 0.440220 4_2:-55900984 Msa0608700:intron
GTCCTTCTTTATCATGATCA+AGG 0.442671 4_2:+55899344 None:intergenic
TCTACAAAGGACTACAAACT+TGG 0.453097 4_2:-55899060 Msa0608700:CDS
TGGGAACAAAACCCAAGAAA+TGG 0.453731 4_2:-55901522 Msa0608700:exon
TACAAGCTAACGAATTGAAC+TGG 0.455479 4_2:+55901059 None:intergenic
TGTAATTGGGCAATAAAGGT+TGG 0.457607 4_2:-55898442 Msa0608700:three_prime_UTR
TATTATATAAGAACATGATT+AGG 0.460279 4_2:-55901600 Msa0608700:five_prime_UTR
AAATTGTTTCGCAGGTTCAA+TGG 0.460433 4_2:-55901148 Msa0608700:intron
AATTCCTAAGAAGAAGACTT+TGG 0.470232 4_2:+55899000 None:intergenic
TTTCCTAAATTTGAAAACAA+TGG 0.479773 4_2:+55898489 None:intergenic
CAAATCAAAAGCTTTAGAAA+AGG 0.480949 4_2:+55898278 None:intergenic
AATGTTACTGACTTCAAAGT+TGG 0.485805 4_2:+55901409 None:intergenic
AAAGATGTAGAGAGAGAAAG+AGG 0.494136 4_2:+55901633 None:intergenic
CAATCAAGAGTGGTGAAGCT+TGG 0.495413 4_2:-55899303 Msa0608700:CDS
AGGGACACCAATGGCTTGGA+TGG 0.502776 4_2:-55898771 Msa0608700:CDS
TTTGAATAGTATATCTTGGA+AGG 0.504986 4_2:-55898341 Msa0608700:three_prime_UTR
TCTTCAACACGTGGCATTGG+TGG 0.505890 4_2:-55899261 Msa0608700:CDS
TAACTTGTTCCGTGGCAAAT+GGG 0.506637 4_2:-55901375 Msa0608700:CDS
GCACATCCTGAAGGTTATAG+TGG 0.507023 4_2:-55898668 Msa0608700:CDS
ATGTAGAGAGAGAAAGAGGA+GGG 0.509507 4_2:+55901637 None:intergenic
CAATGGTTTGAACTTTCTGA+AGG 0.510925 4_2:-55901131 Msa0608700:CDS
ATAACACCACTATAACCTTC+AGG 0.516042 4_2:+55898662 None:intergenic
CGGTTGTGAGCTTCCAGTGT+GGG 0.524560 4_2:+55899237 None:intergenic
TTGGTTTGAAATACCCTGGA+GGG 0.527368 4_2:-55898790 Msa0608700:CDS
TGGTGAAGCTTGGAGAGGAA+TGG 0.527447 4_2:-55899293 Msa0608700:CDS
CTGGAGGGACACCAATGGCT+TGG 0.529625 4_2:-55898775 Msa0608700:CDS
TTCACCACCATCCAAGCCAT+TGG 0.532920 4_2:+55898764 None:intergenic
TCTATGAAAGGACAAGTTGA+AGG 0.533615 4_2:+55901323 None:intergenic
ATAACTTACTGGTAATGTAC+AGG 0.538647 4_2:+55898974 None:intergenic
TGATCTTCAACACGTGGCAT+TGG 0.541024 4_2:-55899264 Msa0608700:CDS
TCATGCATGACTTTGTTGGT+TGG 0.542003 4_2:+55898831 None:intergenic
CAAGTTGAAGGGTTATAGAG+AGG 0.546976 4_2:+55901335 None:intergenic
TTCCAAGTATCAGGAAGTCC+AGG 0.548161 4_2:+55898611 None:intergenic
TAGAATTTGGTACAGCAGCA+TGG 0.554694 4_2:+55901032 None:intergenic
TTCCCACCAAACAAAATCAG+AGG 0.559578 4_2:-55901456 Msa0608700:CDS
GACCTTGATCATGATAAAGA+AGG 0.566545 4_2:-55899346 Msa0608700:CDS
GACTACAAACTTGGGCTAGG+TGG 0.570817 4_2:-55899051 Msa0608700:CDS
ACTACAAACTTGGGCTAGGT+GGG 0.578379 4_2:-55899050 Msa0608700:CDS
AAGCCATTGGTGTCCCTCCA+GGG 0.581911 4_2:+55898777 None:intergenic
CTACAAAGGACTACAAACTT+GGG 0.582833 4_2:-55899059 Msa0608700:CDS
TGGCATTGGTGGACCCACAC+TGG 0.584418 4_2:-55899250 Msa0608700:CDS
ATACCGCACAGCATACTTGG+TGG 0.586879 4_2:-55899368 Msa0608700:CDS
TTGAACTGTGGATCTATGAA+AGG 0.589879 4_2:+55901311 None:intergenic
GGCTTGGATGGTGGTGAACA+AGG 0.591402 4_2:-55898759 Msa0608700:CDS
ATTGGGCAATAAAGGTTGGA+TGG 0.593271 4_2:-55898438 Msa0608700:three_prime_UTR
AATGGTTTGAACTTTCTGAA+GGG 0.597673 4_2:-55901130 Msa0608700:CDS
AGAAAAGATGCACATCCTGA+AGG 0.597997 4_2:-55898677 Msa0608700:CDS
GATGTAGAGAGAGAAAGAGG+AGG 0.602883 4_2:+55901636 None:intergenic
AAGGACTACAAACTTGGGCT+AGG 0.603384 4_2:-55899054 Msa0608700:CDS
GACACCAATGGCTTGGATGG+TGG 0.610440 4_2:-55898768 Msa0608700:CDS
CAGTTCAATTGTCAGAAACA+TGG 0.617132 4_2:-55901296 Msa0608700:CDS
ACTTTCTGAAGGGTAATAGA+GGG 0.617389 4_2:-55901120 Msa0608700:CDS
TGGCAACTGATTGTAGCCAC+TGG 0.618075 4_2:-55898640 Msa0608700:CDS
CTATGAAAGGACAAGTTGAA+GGG 0.623253 4_2:+55901324 None:intergenic
TGGTGAACAAGGTGTTGAGA+AGG 0.632793 4_2:-55898748 Msa0608700:CDS
ATACCCTGGAGGGACACCAA+TGG 0.646199 4_2:-55898780 Msa0608700:CDS
AGCTTTACCAGAAATATAGG+TGG 0.648575 4_2:-55901262 Msa0608700:CDS
ACGGTTGTGAGCTTCCAGTG+TGG 0.657143 4_2:+55899236 None:intergenic
AAGCTTGACTCAATCAAGAG+TGG 0.662012 4_2:-55899313 Msa0608700:CDS
CGTCGTAGACCCATTTGCCA+CGG 0.673142 4_2:+55901366 None:intergenic
AAGAGTGGTGAAGCTTGGAG+AGG 0.679537 4_2:-55899298 Msa0608700:CDS
GGAACATGTAACTTGTTCCG+TGG 0.681608 4_2:-55901383 Msa0608700:CDS
TGTTTCTGACAATTGAACTG+TGG 0.711026 4_2:+55901299 None:intergenic
TTTGGTTTGAAATACCCTGG+AGG 0.715369 4_2:-55898791 Msa0608700:CDS
GAAGTCCAGGAAGACACCAG+TGG 0.726350 4_2:+55898624 None:intergenic
ATGTGTTGATCTTCAACACG+TGG 0.756788 4_2:-55899270 Msa0608700:CDS
GGTCCACCAAGTATGCTGTG+CGG 0.774384 4_2:+55899365 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTATTTTTATTTTGAG+AGG + chr4_2:55899794-55899813 None:intergenic 10.0%
!!! AAAAAAATATTAGGATTTTT+TGG + chr4_2:55900065-55900084 None:intergenic 10.0%
!!! AAATATTTAATTTGATGTTT+TGG - chr4_2:55901426-55901445 Msa0608700:CDS 10.0%
!! TATTATATAAGAACATGATT+AGG - chr4_2:55898295-55898314 Msa0608700:three_prime_UTR 15.0%
!!! GATTTTTTTTGAATGAAAAA+AGG + chr4_2:55898347-55898366 None:intergenic 15.0%
!! AAACAATTAAAAAACACTAT+TGG + chr4_2:55899157-55899176 None:intergenic 15.0%
!!! ATTTTATTTATGATGAAAAG+TGG - chr4_2:55899659-55899678 Msa0608700:intron 15.0%
!!! TATTTTTTTTGTTAAACATC+CGG - chr4_2:55899895-55899914 Msa0608700:intron 15.0%
!!! TAAATGTTATTGTTGTTTTT+AGG - chr4_2:55900482-55900501 Msa0608700:intron 15.0%
!!! TTAAGCTCTAAAAATATATA+TGG - chr4_2:55901013-55901032 Msa0608700:CDS 15.0%
!! ATTCAAATTGAAAACATATT+TGG + chr4_2:55901541-55901560 None:intergenic 15.0%
!!! TTTTCATTCAAAAAAAATCG+TGG - chr4_2:55898348-55898367 Msa0608700:three_prime_UTR 20.0%
!! TTTCATTCAAAAAAAATCGT+GGG - chr4_2:55898349-55898368 Msa0608700:three_prime_UTR 20.0%
!! TTCAATGTAATTACAAATGT+CGG + chr4_2:55899022-55899041 None:intergenic 20.0%
!!! TTTTAAAACAATGAAAGTGA+AGG + chr4_2:55899122-55899141 None:intergenic 20.0%
!!! TTGTTTGTTTTAATGATGTT+AGG - chr4_2:55899179-55899198 Msa0608700:intron 20.0%
!! TAAAATACGTAATGAATTTG+TGG + chr4_2:55899645-55899664 None:intergenic 20.0%
!!! TTTTGAAAATGAAGTAACAA+GGG + chr4_2:55899697-55899716 None:intergenic 20.0%
!!! TTTATTTGACAACGTTTTAT+AGG + chr4_2:55899855-55899874 None:intergenic 20.0%
!!! CAAAGTGTTAATATAAAAAC+CGG + chr4_2:55899917-55899936 None:intergenic 20.0%
!! TTAAATACCAACAAAACTTT+AGG + chr4_2:55899943-55899962 None:intergenic 20.0%
!!! TTTTTTGGAATCCTAAAATT+CGG + chr4_2:55900050-55900069 None:intergenic 20.0%
!! AACATTGAACATTAAGATAA+CGG - chr4_2:55900375-55900394 Msa0608700:intron 20.0%
!! TTAGCATCATTAATTAATCA+TGG + chr4_2:55900726-55900745 None:intergenic 20.0%
!! ATTAGATATTCATAACTTAC+TGG + chr4_2:55900935-55900954 None:intergenic 20.0%
!!! GACAAATTAATTTGTTTGTA+AGG - chr4_2:55901360-55901379 Msa0608700:CDS 20.0%
!!! AATCCATTGTTTTCAAATTT+AGG - chr4_2:55901403-55901422 Msa0608700:CDS 20.0%
!! TTTCCTAAATTTGAAAACAA+TGG + chr4_2:55901409-55901428 None:intergenic 20.0%
!!! ACATTTACAATTTTAGACAT+TGG + chr4_2:55901484-55901503 None:intergenic 20.0%
!! ATTGTAAATGTTCATTGTTT+AGG - chr4_2:55901493-55901512 Msa0608700:CDS 20.0%
!! AACATATTTGGTACAATTAA+AGG + chr4_2:55901529-55901548 None:intergenic 20.0%
!! TCAATTTGAATAGTATATCT+TGG - chr4_2:55901550-55901569 Msa0608700:five_prime_UTR 20.0%
!! TAAAATCCTCTGATTTTGTT+TGG + chr4_2:55898448-55898467 None:intergenic 25.0%
! ATAAGCTTTACCAGAAATAT+AGG - chr4_2:55898630-55898649 Msa0608700:CDS 25.0%
!!! TGTTCTTTTGTTGGTTTTTA+TGG - chr4_2:55898705-55898724 Msa0608700:CDS 25.0%
! CAATAAAGAATTGATGTGAT+TGG - chr4_2:55899369-55899388 Msa0608700:CDS 25.0%
! AAAAAATTTGCCACAAAAGT+GGG + chr4_2:55899477-55899496 None:intergenic 25.0%
! TAAAAAATTTGCCACAAAAG+TGG + chr4_2:55899478-55899497 None:intergenic 25.0%
!!! CTACTCTTTATTTTTTTGAG+AGG - chr4_2:55899557-55899576 Msa0608700:intron 25.0%
! GTTTGAGAAACAATAAAGAA+TGG + chr4_2:55899589-55899608 None:intergenic 25.0%
!!! GTTTTGAAAATGAAGTAACA+AGG + chr4_2:55899698-55899717 None:intergenic 25.0%
!! GTTTTATAGGATAAAGTTCA+AGG + chr4_2:55899842-55899861 None:intergenic 25.0%
! AATTAATCAGATTTACTGGT+AGG + chr4_2:55900243-55900262 None:intergenic 25.0%
! AAGCAATTAATCAGATTTAC+TGG + chr4_2:55900247-55900266 None:intergenic 25.0%
! AATGTGACTGGTATAATTAT+TGG - chr4_2:55900312-55900331 Msa0608700:intron 25.0%
!!! TATTGTTGTTTTTAGGAGTA+TGG - chr4_2:55900489-55900508 Msa0608700:intron 25.0%
!!! ATTGTTGTTTTTAGGAGTAT+GGG - chr4_2:55900490-55900509 Msa0608700:intron 25.0%
! TTGAAAAATTCATGACAGAT+GGG - chr4_2:55900758-55900777 Msa0608700:intron 25.0%
! CTAAAAATATATATGGTGTG+TGG - chr4_2:55901020-55901039 Msa0608700:CDS 25.0%
!!! TAAGGTTCTACTTATTTTAG+AGG - chr4_2:55901378-55901397 Msa0608700:CDS 25.0%
!!! ATGTTTTGGTTCTTGTAATT+GGG - chr4_2:55901440-55901459 Msa0608700:CDS 25.0%
! TTTGAATAGTATATCTTGGA+AGG - chr4_2:55901554-55901573 Msa0608700:five_prime_UTR 25.0%
!!! CAAATCAAAAGCTTTAGAAA+AGG + chr4_2:55901620-55901639 None:intergenic 25.0%
ATTCAAAAAAAATCGTGGGT+TGG - chr4_2:55898353-55898372 Msa0608700:three_prime_UTR 30.0%
TTCAAAAAAAATCGTGGGTT+GGG - chr4_2:55898354-55898373 Msa0608700:three_prime_UTR 30.0%
!! AATGTTACTGACTTCAAAGT+TGG + chr4_2:55898489-55898508 None:intergenic 30.0%
TGGTAACAAAAATTGTACCT+TGG + chr4_2:55898680-55898699 None:intergenic 30.0%
! ACCATTTTGTGTTCTTTTGT+TGG - chr4_2:55898696-55898715 Msa0608700:CDS 30.0%
ACCAACAAAAGAACACAAAA+TGG + chr4_2:55898700-55898719 None:intergenic 30.0%
AATGGTTTGAACTTTCTGAA+GGG - chr4_2:55898765-55898784 Msa0608700:CDS 30.0%
CACAAAAGTCATAAAAGTGA+AGG + chr4_2:55898952-55898971 None:intergenic 30.0%
TTTGAGTATTACAAACCTTG+AGG - chr4_2:55899299-55899318 Msa0608700:CDS 30.0%
AAAGAATTGATGTGATTGGT+TGG - chr4_2:55899373-55899392 Msa0608700:CDS 30.0%
!!! CTTTTTTATTAGAACAGACG+TGG - chr4_2:55899608-55899627 Msa0608700:intron 30.0%
CTAGACAAGATAATGAACTT+TGG - chr4_2:55899767-55899786 Msa0608700:intron 30.0%
!! TTTGACACCTAAAGTTTTGT+TGG - chr4_2:55899933-55899952 Msa0608700:intron 30.0%
! TGAGTAATCAACCGAATTTT+AGG - chr4_2:55900036-55900055 Msa0608700:intron 30.0%
!!! ACGAAACCCTTTTTTTTTCA+TGG - chr4_2:55900148-55900167 Msa0608700:intron 30.0%
ATGGGACCATGAAAAAAAAA+GGG + chr4_2:55900157-55900176 None:intergenic 30.0%
! TTCATTACATTTGCTGAAAC+AGG + chr4_2:55900183-55900202 None:intergenic 30.0%
! TGCTATTTATACAATGTGAC+TGG - chr4_2:55900300-55900319 Msa0608700:intron 30.0%
CTTGAAAAATTCATGACAGA+TGG - chr4_2:55900757-55900776 Msa0608700:intron 30.0%
AATTCCTAAGAAGAAGACTT+TGG + chr4_2:55900898-55900917 None:intergenic 30.0%
! ATAACTTACTGGTAATGTAC+AGG + chr4_2:55900924-55900943 None:intergenic 30.0%
TATATATGGTGTGTGGTATT+AGG - chr4_2:55901027-55901046 Msa0608700:CDS 30.0%
ATATATGGTGTGTGGTATTA+GGG - chr4_2:55901028-55901047 Msa0608700:CDS 30.0%
GGGTATTTCAAACCAAAATA+TGG + chr4_2:55901101-55901120 None:intergenic 30.0%
!!! TATTTTGGTTTGAAATACCC+TGG - chr4_2:55901101-55901120 Msa0608700:CDS 30.0%
GTCACATCTAAGAAATAAAC+TGG + chr4_2:55901182-55901201 None:intergenic 30.0%
!!! GATGTTTTGGTTCTTGTAAT+TGG - chr4_2:55901439-55901458 Msa0608700:CDS 30.0%
TTCTTGTAATTGGGCAATAA+AGG - chr4_2:55901449-55901468 Msa0608700:CDS 30.0%
TAGTATATCTTGGAAGGATT+TGG - chr4_2:55901560-55901579 Msa0608700:five_prime_UTR 30.0%
AAAGATGTAGAGAGAGAAAG+AGG + chr4_2:55898265-55898284 None:intergenic 35.0%
!! AAGCACTGAAACCATTTCTT+GGG + chr4_2:55898387-55898406 None:intergenic 35.0%
!! ATCCTCTGATTTTGTTTGGT+GGG + chr4_2:55898444-55898463 None:intergenic 35.0%
!! AATCCTCTGATTTTGTTTGG+TGG + chr4_2:55898445-55898464 None:intergenic 35.0%
! TTTGAAGTCAGTAACATTGC+AGG - chr4_2:55898491-55898510 Msa0608700:three_prime_UTR 35.0%
! CTATGAAAGGACAAGTTGAA+GGG + chr4_2:55898574-55898593 None:intergenic 35.0%
! TCTATGAAAGGACAAGTTGA+AGG + chr4_2:55898575-55898594 None:intergenic 35.0%
TTGAACTGTGGATCTATGAA+AGG + chr4_2:55898587-55898606 None:intergenic 35.0%
! TGTTTCTGACAATTGAACTG+TGG + chr4_2:55898599-55898618 None:intergenic 35.0%
CAGTTCAATTGTCAGAAACA+TGG - chr4_2:55898599-55898618 Msa0608700:CDS 35.0%
AGCTTTACCAGAAATATAGG+TGG - chr4_2:55898633-55898652 Msa0608700:CDS 35.0%
CTTGGCATGTTACAAGAAAA+TGG + chr4_2:55898662-55898681 None:intergenic 35.0%
! CATTTTCTTGTAACATGCCA+AGG - chr4_2:55898660-55898679 Msa0608700:CDS 35.0%
GTTGTGATAAATTGTTTCGC+AGG - chr4_2:55898739-55898758 Msa0608700:CDS 35.0%
AAATTGTTTCGCAGGTTCAA+TGG - chr4_2:55898747-55898766 Msa0608700:CDS 35.0%
CAATGGTTTGAACTTTCTGA+AGG - chr4_2:55898764-55898783 Msa0608700:CDS 35.0%
! AACTTTCTGAAGGGTAATAG+AGG - chr4_2:55898774-55898793 Msa0608700:CDS 35.0%
! ACTTTCTGAAGGGTAATAGA+GGG - chr4_2:55898775-55898794 Msa0608700:CDS 35.0%
GGGAAGAAGATTATGTTTGT+TGG - chr4_2:55898795-55898814 Msa0608700:CDS 35.0%
TACAAGCTAACGAATTGAAC+TGG + chr4_2:55898839-55898858 None:intergenic 35.0%
!! CTGAATGTGCTTCTAGAATT+TGG + chr4_2:55898879-55898898 None:intergenic 35.0%
TATCTCTTCAGTCACATTTG+AGG - chr4_2:55898911-55898930 Msa0608700:intron 35.0%
! GACTTTTGTGTAATGAAGCA+CGG - chr4_2:55898962-55898981 Msa0608700:intron 35.0%
TATTATCTGTGTTAGCGTGT+CGG - chr4_2:55899061-55899080 Msa0608700:CDS 35.0%
!! TATACTTAGACCCACTTTTG+TGG - chr4_2:55899464-55899483 Msa0608700:intron 35.0%
CATTATGTGCATGTTTGAGT+TGG + chr4_2:55899501-55899520 None:intergenic 35.0%
!! CTTGTCTAGTTGTTTTGCAT+AGG + chr4_2:55899756-55899775 None:intergenic 35.0%
CATGGGACCATGAAAAAAAA+AGG + chr4_2:55900158-55900177 None:intergenic 35.0%
!!! CTTTTTTTTTCATGGTCCCA+TGG - chr4_2:55900156-55900175 Msa0608700:intron 35.0%
TAATCAGATTTACTGGTAGG+TGG + chr4_2:55900240-55900259 None:intergenic 35.0%
TGGTCATAAGTGTATCAAAC+TGG - chr4_2:55900332-55900351 Msa0608700:intron 35.0%
GACCTTGATCATGATAAAGA+AGG - chr4_2:55900549-55900568 Msa0608700:intron 35.0%
GTCCTTCTTTATCATGATCA+AGG + chr4_2:55900554-55900573 None:intergenic 35.0%
AATGTGAGAGAATGATCCTA+CGG + chr4_2:55900681-55900700 None:intergenic 35.0%
TGACAGATGGGATTATATTC+AGG - chr4_2:55900770-55900789 Msa0608700:intron 35.0%
!! CGTTTTGTTGCATTCTACAA+AGG - chr4_2:55900822-55900841 Msa0608700:intron 35.0%
TCTACAAAGGACTACAAACT+TGG - chr4_2:55900835-55900854 Msa0608700:intron 35.0%
CTACAAAGGACTACAAACTT+GGG - chr4_2:55900836-55900855 Msa0608700:intron 35.0%
CAAACCAAAGTCTTCTTCTT+AGG - chr4_2:55900891-55900910 Msa0608700:intron 35.0%
TATATGGTGTGTGGTATTAG+GGG - chr4_2:55901029-55901048 Msa0608700:CDS 35.0%
!!! AGTGAGAAAGTGCCATATTT+TGG - chr4_2:55901086-55901105 Msa0608700:CDS 35.0%
ATAACACCACTATAACCTTC+AGG + chr4_2:55901236-55901255 None:intergenic 35.0%
! TGAAGGTTATAGTGGTGTTA+TGG - chr4_2:55901235-55901254 Msa0608700:intron 35.0%
TGTAATTGGGCAATAAAGGT+TGG - chr4_2:55901453-55901472 Msa0608700:CDS 35.0%
ATGTAGAGAGAGAAAGAGGA+GGG + chr4_2:55898261-55898280 None:intergenic 40.0%
TGGGAACAAAACCCAAGAAA+TGG - chr4_2:55898373-55898392 Msa0608700:three_prime_UTR 40.0%
!! CAAGCACTGAAACCATTTCT+TGG + chr4_2:55898388-55898407 None:intergenic 40.0%
TTCCCACCAAACAAAATCAG+AGG - chr4_2:55898439-55898458 Msa0608700:three_prime_UTR 40.0%
!! TAACTTGTTCCGTGGCAAAT+GGG - chr4_2:55898520-55898539 Msa0608700:three_prime_UTR 40.0%
! CAAGTTGAAGGGTTATAGAG+AGG + chr4_2:55898563-55898582 None:intergenic 40.0%
ATGGCATCCACCTATATTTC+TGG + chr4_2:55898643-55898662 None:intergenic 40.0%
TAGAATTTGGTACAGCAGCA+TGG + chr4_2:55898866-55898885 None:intergenic 40.0%
! TCTTCAGTCACATTTGAGGT+TGG - chr4_2:55898915-55898934 Msa0608700:intron 40.0%
!! TACAAATGTCGGTGTTGTGT+CGG + chr4_2:55899011-55899030 None:intergenic 40.0%
ACCTTGAGGTTAGAACATGA+TGG - chr4_2:55899313-55899332 Msa0608700:CDS 40.0%
ACCATCATGTTCTAACCTCA+AGG + chr4_2:55899317-55899336 None:intergenic 40.0%
! GAATTGATGTGATTGGTTGG+AGG - chr4_2:55899376-55899395 Msa0608700:CDS 40.0%
AGAAAATGTCCCCAACACAA+AGG + chr4_2:55899406-55899425 None:intergenic 40.0%
! ATTTGCTGAAACAGGACCAT+GGG + chr4_2:55900175-55900194 None:intergenic 40.0%
AAGCTTGACTCAATCAAGAG+TGG - chr4_2:55900582-55900601 Msa0608700:intron 40.0%
! ATGTGTTGATCTTCAACACG+TGG - chr4_2:55900625-55900644 Msa0608700:intron 40.0%
!! AAGAAGACTTTGGTTTGTGC+TGG + chr4_2:55900888-55900907 None:intergenic 40.0%
ATATGGTGTGTGGTATTAGG+GGG - chr4_2:55901030-55901049 Msa0608700:CDS 40.0%
TCATGCATGACTTTGTTGGT+TGG + chr4_2:55901067-55901086 None:intergenic 40.0%
TCACTCATGCATGACTTTGT+TGG + chr4_2:55901071-55901090 None:intergenic 40.0%
!! TTTGGTTTGAAATACCCTGG+AGG - chr4_2:55901104-55901123 Msa0608700:CDS 40.0%
! TTGGTTTGAAATACCCTGGA+GGG - chr4_2:55901105-55901124 Msa0608700:CDS 40.0%
AGAAAAGATGCACATCCTGA+AGG - chr4_2:55901218-55901237 Msa0608700:intron 40.0%
! AGAAGCTGATTCCAAGTATC+AGG + chr4_2:55901296-55901315 None:intergenic 40.0%
ATTGGGCAATAAAGGTTGGA+TGG - chr4_2:55901457-55901476 Msa0608700:CDS 40.0%
GATGTAGAGAGAGAAAGAGG+AGG + chr4_2:55898262-55898281 None:intergenic 45.0%
!! GGAACATGTAACTTGTTCCG+TGG - chr4_2:55898512-55898531 Msa0608700:three_prime_UTR 45.0%
!! GTAACTTGTTCCGTGGCAAA+TGG - chr4_2:55898519-55898538 Msa0608700:three_prime_UTR 45.0%
GCACGGACACTCTTTGAATT+AGG - chr4_2:55898979-55898998 Msa0608700:intron 45.0%
!! ACAAATGTCGGTGTTGTGTC+GGG + chr4_2:55899010-55899029 None:intergenic 45.0%
AACGAACATCAGACACGACA+TGG + chr4_2:55899090-55899109 None:intergenic 45.0%
TTGGAGGCTTTCCTTTGTGT+TGG - chr4_2:55899392-55899411 Msa0608700:CDS 45.0%
TGGAGGCTTTCCTTTGTGTT+GGG - chr4_2:55899393-55899412 Msa0608700:CDS 45.0%
! CATTTGCTGAAACAGGACCA+TGG + chr4_2:55900176-55900195 None:intergenic 45.0%
CAGATTTACTGGTAGGTGGT+AGG + chr4_2:55900236-55900255 None:intergenic 45.0%
TCTATACCGCACAGCATACT+TGG - chr4_2:55900524-55900543 Msa0608700:intron 45.0%
CAATCAAGAGTGGTGAAGCT+TGG - chr4_2:55900592-55900611 Msa0608700:intron 45.0%
TGATCTTCAACACGTGGCAT+TGG - chr4_2:55900631-55900650 Msa0608700:intron 45.0%
AAGGACTACAAACTTGGGCT+AGG - chr4_2:55900841-55900860 Msa0608700:intron 45.0%
! ACTACAAACTTGGGCTAGGT+GGG - chr4_2:55900845-55900864 Msa0608700:intron 45.0%
!! TGGTGAACAAGGTGTTGAGA+AGG - chr4_2:55901147-55901166 Msa0608700:intron 45.0%
GCACATCCTGAAGGTTATAG+TGG - chr4_2:55901227-55901246 Msa0608700:intron 45.0%
TTCCTGGACTTCCTGATACT+TGG - chr4_2:55901282-55901301 Msa0608700:CDS 45.0%
TTCCAAGTATCAGGAAGTCC+AGG + chr4_2:55901287-55901306 None:intergenic 45.0%
!! TAATGAATTAAAAAAATATT+AGG + chr4_2:55900074-55900093 None:intergenic 5.0%
GGAGGCTTTCCTTTGTGTTG+GGG - chr4_2:55899394-55899413 Msa0608700:CDS 50.0%
ATACCGCACAGCATACTTGG+TGG - chr4_2:55900527-55900546 Msa0608700:intron 50.0%
AAGAGTGGTGAAGCTTGGAG+AGG - chr4_2:55900597-55900616 Msa0608700:intron 50.0%
TGGTGAAGCTTGGAGAGGAA+TGG - chr4_2:55900602-55900621 Msa0608700:intron 50.0%
! TCTTCAACACGTGGCATTGG+TGG - chr4_2:55900634-55900653 Msa0608700:intron 50.0%
! GACTACAAACTTGGGCTAGG+TGG - chr4_2:55900844-55900863 Msa0608700:intron 50.0%
GTGGTATTAGGGGGAGAGAT+TGG - chr4_2:55901039-55901058 Msa0608700:CDS 50.0%
TTCACCACCATCCAAGCCAT+TGG + chr4_2:55901134-55901153 None:intergenic 50.0%
TGGCAACTGATTGTAGCCAC+TGG - chr4_2:55901255-55901274 Msa0608700:CDS 50.0%
CGTCGTAGACCCATTTGCCA+CGG + chr4_2:55898532-55898551 None:intergenic 55.0%
GGTCCACCAAGTATGCTGTG+CGG + chr4_2:55900533-55900552 None:intergenic 55.0%
CGGTTGTGAGCTTCCAGTGT+GGG + chr4_2:55900661-55900680 None:intergenic 55.0%
ACGGTTGTGAGCTTCCAGTG+TGG + chr4_2:55900662-55900681 None:intergenic 55.0%
CACTGGAAGCTCACAACCGT+AGG - chr4_2:55900662-55900681 Msa0608700:intron 55.0%
ATACCCTGGAGGGACACCAA+TGG - chr4_2:55901115-55901134 Msa0608700:CDS 55.0%
! AAGCCATTGGTGTCCCTCCA+GGG + chr4_2:55901121-55901140 None:intergenic 55.0%
! AGGGACACCAATGGCTTGGA+TGG - chr4_2:55901124-55901143 Msa0608700:CDS 55.0%
! GACACCAATGGCTTGGATGG+TGG - chr4_2:55901127-55901146 Msa0608700:CDS 55.0%
! GGCTTGGATGGTGGTGAACA+AGG - chr4_2:55901136-55901155 Msa0608700:CDS 55.0%
TGTAGCCACTGGTGTCTTCC+TGG - chr4_2:55901266-55901285 Msa0608700:CDS 55.0%
GAAGTCCAGGAAGACACCAG+TGG + chr4_2:55901274-55901293 None:intergenic 55.0%
!! TGGCATTGGTGGACCCACAC+TGG - chr4_2:55900645-55900664 Msa0608700:intron 60.0%
! CAAGCCATTGGTGTCCCTCC+AGG + chr4_2:55901122-55901141 None:intergenic 60.0%
CTGGAGGGACACCAATGGCT+TGG - chr4_2:55901120-55901139 Msa0608700:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 55898253 55901664 55898253 ID=Msa0608700;Name=Msa0608700
chr4_2 mRNA 55898253 55901664 55898253 ID=Msa0608700-mRNA-1;Parent=Msa0608700;Name=Msa0608700-mRNA-1;_AED=0.09;_eAED=0.09;_QI=139|1|1|1|1|1|5|327|361
chr4_2 exon 55898253 55898868 55898253 ID=Msa0608700-mRNA-1:exon:14349;Parent=Msa0608700-mRNA-1
chr4_2 exon 55898983 55899141 55898983 ID=Msa0608700-mRNA-1:exon:14348;Parent=Msa0608700-mRNA-1
chr4_2 exon 55899238 55899413 55899238 ID=Msa0608700-mRNA-1:exon:14347;Parent=Msa0608700-mRNA-1
chr4_2 exon 55900985 55901156 55900985 ID=Msa0608700-mRNA-1:exon:14346;Parent=Msa0608700-mRNA-1
chr4_2 exon 55901236 55901664 55901236 ID=Msa0608700-mRNA-1:exon:14345;Parent=Msa0608700-mRNA-1
chr4_2 five_prime_UTR 55901526 55901664 55901526 ID=Msa0608700-mRNA-1:five_prime_utr;Parent=Msa0608700-mRNA-1
chr4_2 CDS 55901236 55901525 55901236 ID=Msa0608700-mRNA-1:cds;Parent=Msa0608700-mRNA-1
chr4_2 CDS 55900985 55901156 55900985 ID=Msa0608700-mRNA-1:cds;Parent=Msa0608700-mRNA-1
chr4_2 CDS 55899238 55899413 55899238 ID=Msa0608700-mRNA-1:cds;Parent=Msa0608700-mRNA-1
chr4_2 CDS 55898983 55899141 55898983 ID=Msa0608700-mRNA-1:cds;Parent=Msa0608700-mRNA-1
chr4_2 CDS 55898580 55898868 55898580 ID=Msa0608700-mRNA-1:cds;Parent=Msa0608700-mRNA-1
chr4_2 three_prime_UTR 55898253 55898579 55898253 ID=Msa0608700-mRNA-1:three_prime_utr;Parent=Msa0608700-mRNA-1
Gene Sequence

>Msa0608700

ATGGTTTCAGTGCTTGTAAATGCTCTGCTTCTAACTCTGTTTCTGTTTTCCCACCAAACAAAATCAGAGGATTTTAACTCTTTCAGCAATGAGACCAACTTTGAAGTCAGTAACATTGCAGGAACATGTAACTTGTTCCGTGGCAAATGGGTCTACGACGCTTCATATCCTCTCTATAACCCTTCAACTTGTCCTTTCATAGATCCACAGTTCAATTGTCAGAAACATGGTCGCAAAGATAAGCTTTACCAGAAATATAGGTGGATGCCATTTTCTTGTAACATGCCAAGGTTCAATGGTTTGAACTTTCTGAAGGGTAATAGAGGGAAGAAGATTATGTTTGTTGGTGACTCTTTGAGCTTAAACCAGTTCAATTCGTTAGCTTGTATGATCCATGCTGCTGTACCAAATTCTAGAAGCACATTCAGACAAAGAGATGCTATCTCTTCAGTCACATTTGAGGAGTATGGGCTTGAGTTATTTCTATACCGCACAGCATACTTGGTGGACCTTGATCATGATAAAGAAGGACGAGTATTGAAGCTTGACTCAATCAAGAGTGGTGAAGCTTGGAGAGGAATGGATGTGTTGATCTTCAACACGTGGCATTGGTGGACCCACACTGGAAGCTCACAACCATGGGATTATATTCAGGAGAATAAAAAGTTGTATAAAGACATGAATCGTTTTGTTGCATTCTACAAAGGACTACAAACTTGGGCTAGGTGGGTTGAAATGAATGTTAATCCAGCACAAACCAAAGTCTTCTTCTTAGGAATTTCTCCTGTACATTACCAGGGGAGAGATTGGAACCAACCAACAAAGTCATGCATGAGTGAGAAAGTGCCATATTTTGGTTTGAAATACCCTGGAGGGACACCAATGGCTTGGATGGTGGTGAACAAGGTGTTGAGAAGGATGAGTAAGCCAGTTTATTTCTTAGATGTGACAACTCTTTCACAATACAGAAAAGATGCACATCCTGAAGGTTATAGTGGTGTTATGGCAACTGATTGTAGCCACTGGTGTCTTCCTGGACTTCCTGATACTTGGAATCAGCTTCTCTATGCAGCACTTTCTCATTGA
Protein sequence

>Msa0608700

MVSVLVNALLLTLFLFSHQTKSEDFNSFSNETNFEVSNIAGTCNLFRGKWVYDASYPLYNPSTCPFIDPQFNCQKHGRKDKLYQKYRWMPFSCNMPRFNGLNFLKGNRGKKIMFVGDSLSLNQFNSLACMIHAAVPNSRSTFRQRDAISSVTFEEYGLELFLYRTAYLVDLDHDKEGRVLKLDSIKSGEAWRGMDVLIFNTWHWWTHTGSSQPWDYIQENKKLYKDMNRFVAFYKGLQTWARWVEMNVNPAQTKVFFLGISPVHYQGRDWNQPTKSCMSEKVPYFGLKYPGGTPMAWMVVNKVLRRMSKPVYFLDVTTLSQYRKDAHPEGYSGVMATDCSHWCLPGLPDTWNQLLYAALSH*