AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene002470

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene002470.t1 MTR_2g014030 100.000 248 0 0 1 248 1 248 0.0 501
MS.gene002470.t1 MTR_4g088595 96.774 248 8 0 1 248 1 248 2.02e-176 485
MS.gene002470.t1 MTR_7g053160 95.968 248 10 0 1 248 1 248 5.74e-175 481
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene002470.t1 AT5G10360 92.562 242 18 0 1 242 1 242 1.63e-163 452
MS.gene002470.t1 AT4G31700 92.149 242 19 0 1 242 1 242 4.08e-155 431
MS.gene002470.t1 AT5G10360 73.967 242 11 1 1 242 1 190 1.18e-118 337
MS.gene002470.t1 AT4G31700 91.667 180 15 0 63 242 1 180 1.14e-106 306

Find 73 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGAAGAGCTTACGGATTT+TGG 0.153100 2.1:+70955656 None:intergenic
TAAATTGTAGTCGTGCATTT+TGG 0.161193 2.1:-70956999 MS.gene002470:intron
TTGCTACTCGCAGGAATTTA+AGG 0.172205 2.1:-70956854 MS.gene002470:intron
CTCTTAGGACCCCTCATTCT+TGG 0.233777 2.1:+70955684 None:intergenic
GAGGATCTCACAAGAGGTTT+TGG 0.240752 2.1:-70956971 MS.gene002470:CDS
CATCGATTTCAAGCTTCTTC+TGG 0.245851 2.1:+70957121 None:intergenic
GTGACCAGGCGCTGTATCTT+TGG 0.280234 2.1:+70954536 None:intergenic
TGACCAGGCGCTGTATCTTT+GGG 0.285753 2.1:+70954537 None:intergenic
TTCCCTTTGCTCCTTCAATC+TGG 0.299462 2.1:+70954412 None:intergenic
GGAAGAAACTTTACCTGCTT+TGG 0.319600 2.1:+70955575 None:intergenic
GTTAACACACCCTGCTTCAT+TGG 0.338665 2.1:+70956787 None:intergenic
GGAACGCCGCAGTGAGAGTT+TGG 0.366197 2.1:-70954393 MS.gene002470:CDS
TGCTACTCGCAGGAATTTAA+GGG 0.399045 2.1:-70956853 MS.gene002470:intron
ACTTCTTGCCTCCAGATTGA+AGG 0.405331 2.1:-70954423 MS.gene002470:CDS
AGACCTCTCTGTTCTGAACT+TGG 0.408965 2.1:-70955760 MS.gene002470:CDS
GGCGTAATGGAGAGAGAAGA+AGG 0.430202 2.1:-70955812 MS.gene002470:CDS
TGGAAAGATTGAAGAGCTTA+CGG 0.437945 2.1:+70955649 None:intergenic
CTCTTTCTTTGAAGAGTCAA+AGG 0.440470 2.1:+70954512 None:intergenic
AAGTTCAGAACAGAGAGGTC+TGG 0.450666 2.1:+70955762 None:intergenic
CTTTGACTCTTCAAAGAAAG+AGG 0.462251 2.1:-70954511 MS.gene002470:CDS
CTTCCTGACATCATCATCCT+TGG 0.467618 2.1:+70955629 None:intergenic
AGCAAGAGGCGAACACGACC+AGG 0.471596 2.1:+70956763 None:intergenic
ACAAGGATTTCCAATGAAGC+AGG 0.480359 2.1:-70956797 MS.gene002470:CDS
GAACTTGGTTATTGTGAAGA+AGG 0.484415 2.1:-70955745 MS.gene002470:CDS
CGAGGACACGGAAGGCGTAA+TGG 0.492499 2.1:-70955825 MS.gene002470:CDS
TGCTGCAACACCTTGCTTAG+CGG 0.498477 2.1:+70954346 None:intergenic
AAGGGTGAAAATGATTTGCC+AGG 0.499262 2.1:-70955726 MS.gene002470:CDS
AGTTCAGAACAGAGAGGTCT+GGG 0.504245 2.1:+70955763 None:intergenic
CTCCAGATTGAAGGAGCAAA+GGG 0.505775 2.1:-70954414 MS.gene002470:CDS
CCTTTGCTCCTTCAATCTGG+AGG 0.510518 2.1:+70954415 None:intergenic
TTTCCAAGGATGATGATGTC+AGG 0.517231 2.1:-70955632 MS.gene002470:CDS
GACCAGGCGCTGTATCTTTG+GGG 0.517909 2.1:+70954538 None:intergenic
TTCTTCTGGCAACCAGTGGT+TGG 0.519589 2.1:+70957135 None:intergenic
AACGTTGCAAATCCAACCAC+TGG 0.533153 2.1:-70957147 MS.gene002470:CDS
GTTTGGCTAAGAAGAGATCA+AGG 0.534691 2.1:-70954376 MS.gene002470:CDS
ATGTTGAAAAGCCAAGAATG+AGG 0.544680 2.1:-70955695 MS.gene002470:CDS
AGCCCCAAAGATACAGCGCC+TGG 0.547274 2.1:-70954540 MS.gene002470:CDS
AAGCTTCTTCTGGCAACCAG+TGG 0.547317 2.1:+70957131 None:intergenic
CTTCCGTGTCCTCGGAAACA+AGG 0.549864 2.1:+70955834 None:intergenic
CATTACGCCTTCCGTGTCCT+CGG 0.552842 2.1:+70955826 None:intergenic
CGGACTTTCACAACCAAAGC+AGG 0.557230 2.1:-70955588 MS.gene002470:intron
AGAAGAAGGAAGTCTGTTCG+TGG 0.558714 2.1:-70955798 MS.gene002470:CDS
GGATACGTCTTCAAAATCAC+TGG 0.563795 2.1:-70956832 MS.gene002470:CDS
AAGCAGGGTGTGTTAACCCC+TGG 0.565983 2.1:-70956781 MS.gene002470:CDS
TGTTGAAAAGCCAAGAATGA+GGG 0.570225 2.1:-70955694 MS.gene002470:CDS
ACTCCTTGTTTCCGAGGACA+CGG 0.574407 2.1:-70955837 MS.gene002470:CDS
TTTGACTCTTCAAAGAAAGA+GGG 0.574504 2.1:-70954510 MS.gene002470:CDS
CCTCCAGATTGAAGGAGCAA+AGG 0.577181 2.1:-70954415 MS.gene002470:CDS
TAAGCTCTTCAATCTTTCCA+AGG 0.582836 2.1:-70955646 MS.gene002470:CDS
TACGTCTTCAAAATCACTGG+TGG 0.593011 2.1:-70956829 MS.gene002470:CDS
AACTTGGTTATTGTGAAGAA+GGG 0.597761 2.1:-70955744 MS.gene002470:CDS
GCAGGTACTCCTTGTTTCCG+AGG 0.602952 2.1:-70955843 MS.gene002470:intron
GAAGAGTCAAAGGAGTGACC+AGG 0.604666 2.1:+70954522 None:intergenic
TTATACCTCTGTGGAGCAAG+AGG 0.605499 2.1:+70956749 None:intergenic
CTTTGGTTGTGAAAGTCCGA+CGG 0.609736 2.1:+70955592 None:intergenic
AGGCTCTCCACCGCTAAGCA+AGG 0.611423 2.1:-70954356 MS.gene002470:CDS
GGACAAGAGGATCTCACAAG+AGG 0.614846 2.1:-70956977 MS.gene002470:CDS
CTTGTTTCCGAGGACACGGA+AGG 0.615260 2.1:-70955833 MS.gene002470:CDS
GCAAGAGGCGAACACGACCA+GGG 0.615545 2.1:+70956764 None:intergenic
GGTTGTGAAAGTCCGACGGT+AGG 0.616762 2.1:+70955596 None:intergenic
GAAGAAGGAAGTCTGTTCGT+GGG 0.631315 2.1:-70955797 MS.gene002470:CDS
ACTGGTGGGTGTGACAAACA+AGG 0.636404 2.1:-70956814 MS.gene002470:CDS
GTTGAAAAGCCAAGAATGAG+GGG 0.636999 2.1:-70955693 MS.gene002470:CDS
TGCAACACCTTGCTTAGCGG+TGG 0.641230 2.1:+70954349 None:intergenic
CAAGGATTTCCAATGAAGCA+GGG 0.641279 2.1:-70956796 MS.gene002470:CDS
ACGTCTTCAAAATCACTGGT+GGG 0.655439 2.1:-70956828 MS.gene002470:CDS
GTAGAAAGGTTATACCTCTG+TGG 0.668019 2.1:+70956740 None:intergenic
AATTGCCAAAGCAAAATCAG+AGG 0.670645 2.1:-70954462 MS.gene002470:CDS
CAAGAGGCGAACACGACCAG+GGG 0.676279 2.1:+70956765 None:intergenic
AGTATGTCAACACCTACCGT+CGG 0.698935 2.1:-70955608 MS.gene002470:CDS
GTTCGCCTCTTGCTCCACAG+AGG 0.710024 2.1:-70956754 MS.gene002470:intron
TCTTAGCCAAACTCTCACTG+CGG 0.768400 2.1:+70954387 None:intergenic
TAACCAAGTTCAGAACAGAG+AGG 0.775773 2.1:+70955757 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAATAAAAATAAAAATT+AGG + chr2.1:70954564-70954583 None:intergenic 0.0%
!! TAATTAATATACAAAACAAA+AGG + chr2.1:70956411-70956430 None:intergenic 10.0%
!! TCAATATAACAATTAATTTA+AGG + chr2.1:70954687-70954706 None:intergenic 10.0%
!!! TTTTATTAATTATTATAGTG+AGG - chr2.1:70954887-70954906 MS.gene002470:intron 10.0%
!! AAAAAAAAAAAACTGTAGAA+AGG + chr2.1:70954858-70954877 None:intergenic 15.0%
!! ATATTAATTATGCTATGTTA+TGG - chr2.1:70956421-70956440 MS.gene002470:intron 15.0%
!! GATTTATTAATTATGTTGTT+CGG - chr2.1:70955355-70955374 MS.gene002470:intron 15.0%
!! GTATATATGTATGTATAAAT+TGG + chr2.1:70955424-70955443 None:intergenic 15.0%
!! TATGGATCTATAATTTATAA+TGG - chr2.1:70956965-70956984 MS.gene002470:CDS 15.0%
!!! AAAAAAAGATCTAAAATAGA+GGG + chr2.1:70955190-70955209 None:intergenic 15.0%
!!! TTCTAATAGAATTTTATCTA+AGG - chr2.1:70956935-70956954 MS.gene002470:intron 15.0%
!! AAAAATTCCAACTTAGATAT+TGG + chr2.1:70956129-70956148 None:intergenic 20.0%
!! AAGGCAATAAAAATTGAAAA+AGG + chr2.1:70954511-70954530 None:intergenic 20.0%
!! ACAAGTGATAAATACTAAAT+AGG + chr2.1:70956094-70956113 None:intergenic 20.0%
!! ACCAAATAAATACTTAACAA+GGG + chr2.1:70955693-70955712 None:intergenic 20.0%
!! ACTGCAAAATAATTTCTATA+TGG + chr2.1:70954412-70954431 None:intergenic 20.0%
!! CAAACAGTAATAAAAACAAA+AGG + chr2.1:70955128-70955147 None:intergenic 20.0%
!! CATAATTAACAAAACTGAAA+AGG + chr2.1:70955312-70955331 None:intergenic 20.0%
!! TACCAAATAAATACTTAACA+AGG + chr2.1:70955694-70955713 None:intergenic 20.0%
!!! ATCACTTGCTATTATAATAT+TGG - chr2.1:70956153-70956172 MS.gene002470:intron 20.0%
!!! GAAAAAAAGATCTAAAATAG+AGG + chr2.1:70955191-70955210 None:intergenic 20.0%
!!! TAGATCTTTTTTTCACTAAT+AGG - chr2.1:70955197-70955216 MS.gene002470:intron 20.0%
!!! TCACTTGCTATTATAATATT+GGG - chr2.1:70956154-70956173 MS.gene002470:intron 20.0%
! AATTTAATGTTTGTTTGAGC+AGG - chr2.1:70955720-70955739 MS.gene002470:CDS 25.0%
! ACCCTTGTTAAGTATTTATT+TGG - chr2.1:70955689-70955708 MS.gene002470:CDS 25.0%
! AGATTTAAAAATAGAGTCAG+AGG + chr2.1:70956706-70956725 None:intergenic 25.0%
! ATGACATTAACAATACATAC+AGG + chr2.1:70956221-70956240 None:intergenic 25.0%
! ATTATAATATTGGGTGAATC+TGG - chr2.1:70956163-70956182 MS.gene002470:intron 25.0%
! CATAATAGCAATGAAATCAA+AGG + chr2.1:70954383-70954402 None:intergenic 25.0%
! CTCTTTATCATTTACTTGAA+TGG - chr2.1:70955100-70955119 MS.gene002470:intron 25.0%
! GATTTAAAAATAGAGTCAGA+GGG + chr2.1:70956705-70956724 None:intergenic 25.0%
! TTATTAATTATGTTGTTCGG+TGG - chr2.1:70955358-70955377 MS.gene002470:intron 25.0%
!! TAGCTTCTTTCAATTTTTCA+AGG + chr2.1:70956337-70956356 None:intergenic 25.0%
!! TATGTTATGGTTAGAAGTAT+AGG - chr2.1:70956434-70956453 MS.gene002470:intron 25.0%
!! TGTCAATTTGCAATTGTTTT+GGG + chr2.1:70956729-70956748 None:intergenic 25.0%
!! TTGTCAATTTGCAATTGTTT+TGG + chr2.1:70956730-70956749 None:intergenic 25.0%
!! TTTATCTAAGGTCATAACTA+TGG - chr2.1:70956947-70956966 MS.gene002470:intron 25.0%
!!! TACTATAAACCCATTTTTGT+TGG - chr2.1:70954936-70954955 MS.gene002470:intron 25.0%
!!! TGTTGGTGAATTTTTTTTGT+AGG - chr2.1:70955248-70955267 MS.gene002470:intron 25.0%
!!! TTCAATTTTTCAAGGATTGA+TGG + chr2.1:70956329-70956348 None:intergenic 25.0%
!!! TTGGTTTTGAAAAATGTTCT+TGG - chr2.1:70956061-70956080 MS.gene002470:intron 25.0%
AAATCGTGTGAATTGAAGAA+AGG + chr2.1:70954530-70954549 None:intergenic 30.0%
AACTTGGTTATTGTGAAGAA+GGG - chr2.1:70955837-70955856 MS.gene002470:CDS 30.0%
ACAAAATAAGTTCACCAGTT+TGG + chr2.1:70955227-70955246 None:intergenic 30.0%
ACACAAACTCATCTATACAA+TGG + chr2.1:70956269-70956288 None:intergenic 30.0%
AGATTGCTAAATAGATGAAG+AGG - chr2.1:70956608-70956627 MS.gene002470:intron 30.0%
AGTGAATGCAAGAGAATATT+TGG - chr2.1:70956543-70956562 MS.gene002470:intron 30.0%
AGTGATATTCACAGATAATC+AGG + chr2.1:70956030-70956049 None:intergenic 30.0%
AGTTAGTTGGAAAGTAGTTT+AGG - chr2.1:70956512-70956531 MS.gene002470:intron 30.0%
ATTGTCTCCAATATCTAAGT+TGG - chr2.1:70956119-70956138 MS.gene002470:intron 30.0%
CAAAATAAGTTCACCAGTTT+GGG + chr2.1:70955226-70955245 None:intergenic 30.0%
CAATGAAATCAAAGGAACAA+GGG + chr2.1:70954375-70954394 None:intergenic 30.0%
CACAAACTCATCTATACAAT+GGG + chr2.1:70956268-70956287 None:intergenic 30.0%
CTAAATTAGCCCATCTAAAT+TGG + chr2.1:70955026-70955045 None:intergenic 30.0%
GATTGCTAAATAGATGAAGA+GGG - chr2.1:70956609-70956628 MS.gene002470:intron 30.0%
GTTAACATTAGCAAGTTAGT+TGG - chr2.1:70956499-70956518 MS.gene002470:intron 30.0%
TGTACTATTGTTTGCGTTAT+AGG - chr2.1:70955068-70955087 MS.gene002470:intron 30.0%
TGTTTGAATTATTCGTTTCC+TGG - chr2.1:70955142-70955161 MS.gene002470:intron 30.0%
TTTGACTCTTCAAAGAAAGA+GGG - chr2.1:70957071-70957090 MS.gene002470:intron 30.0%
! AAATTGTAGTCGTGCATTTT+GGG - chr2.1:70954583-70954602 MS.gene002470:intron 30.0%
! AACATATCCGCTCTTTTATT+TGG - chr2.1:70956847-70956866 MS.gene002470:intron 30.0%
! TAAATTGTAGTCGTGCATTT+TGG - chr2.1:70954582-70954601 MS.gene002470:intron 30.0%
! TCTAAAATAGAGGGTTAAAC+CGG + chr2.1:70955181-70955200 None:intergenic 30.0%
! TTTAAAATACCAGCGAGAAT+GGG - chr2.1:70955524-70955543 MS.gene002470:intron 30.0%
!! ACATATCCGCTCTTTTATTT+GGG - chr2.1:70956848-70956867 MS.gene002470:intron 30.0%
!! ATTTTGGGGAAAAACATCAA+TGG + chr2.1:70955391-70955410 None:intergenic 30.0%
!!! ACAAAAGGTTTTAAGTTGAG+TGG + chr2.1:70956396-70956415 None:intergenic 30.0%
!!! CAAAAGGTTTTAAGTTGAGT+GGG + chr2.1:70956395-70956414 None:intergenic 30.0%
!!! GAATATTTGGTTGTTTTGAG+AGG - chr2.1:70956556-70956575 MS.gene002470:intron 30.0%
!!! GGTGAACTTATTTTGTATGT+TGG - chr2.1:70955231-70955250 MS.gene002470:intron 30.0%
!!! TTTTAAAATACCAGCGAGAA+TGG - chr2.1:70955523-70955542 MS.gene002470:intron 30.0%
AAGAGAATCGTCTACCATTA+GGG + chr2.1:70955656-70955675 None:intergenic 35.0%
AATTGCCAAAGCAAAATCAG+AGG - chr2.1:70957119-70957138 MS.gene002470:CDS 35.0%
ACAACAACTGAATTGCAACA+GGG - chr2.1:70956675-70956694 MS.gene002470:intron 35.0%
ATAAGTCCTCTGATAGAACA+AGG + chr2.1:70956914-70956933 None:intergenic 35.0%
ATGTTGAAAAGCCAAGAATG+AGG - chr2.1:70955886-70955905 MS.gene002470:intron 35.0%
CAAAGGAACAAGGGAAATTA+CGG + chr2.1:70954366-70954385 None:intergenic 35.0%
CCATCATCACCAACAAAAAT+GGG + chr2.1:70954948-70954967 None:intergenic 35.0%
CTAGTTCCAATTAACAGCAA+CGG + chr2.1:70956641-70956660 None:intergenic 35.0%
CTTTCATAGACTTGCATGAT+AGG - chr2.1:70956882-70956901 MS.gene002470:intron 35.0%
CTTTGACTCTTCAAAGAAAG+AGG - chr2.1:70957070-70957089 MS.gene002470:intron 35.0%
GAACTTGGTTATTGTGAAGA+AGG - chr2.1:70955836-70955855 MS.gene002470:CDS 35.0%
GCAATGAAATCAAAGGAACA+AGG + chr2.1:70954376-70954395 None:intergenic 35.0%
TAAGAGAATCGTCTACCATT+AGG + chr2.1:70955657-70955676 None:intergenic 35.0%
TAAGCTCTTCAATCTTTCCA+AGG - chr2.1:70955935-70955954 MS.gene002470:intron 35.0%
TAATATTGGGTGAATCTGGT+CGG - chr2.1:70956167-70956186 MS.gene002470:intron 35.0%
TAGTTCCAATTAACAGCAAC+GGG + chr2.1:70956640-70956659 None:intergenic 35.0%
TGGAAAGATTGAAGAGCTTA+CGG + chr2.1:70955935-70955954 None:intergenic 35.0%
TGTTGAAAAGCCAAGAATGA+GGG - chr2.1:70955887-70955906 MS.gene002470:intron 35.0%
TTAAAATACCAGCGAGAATG+GGG - chr2.1:70955525-70955544 MS.gene002470:intron 35.0%
! AATTGGCAGCTGACAAATTT+TGG + chr2.1:70955407-70955426 None:intergenic 35.0%
! ATATTCCCGTTGCTGTTAAT+TGG - chr2.1:70956632-70956651 MS.gene002470:intron 35.0%
! ATTGAAGAGCTTACGGATTT+TGG + chr2.1:70955928-70955947 None:intergenic 35.0%
! ATTGGCAGCTGACAAATTTT+GGG + chr2.1:70955406-70955425 None:intergenic 35.0%
! CTCTTTCTTTGAAGAGTCAA+AGG + chr2.1:70957072-70957091 None:intergenic 35.0%
! TTTTCAACATCAGTCAAACC+TGG + chr2.1:70955876-70955895 None:intergenic 35.0%
!! TTTTCAAGGATTGATGGCAA+GGG + chr2.1:70956323-70956342 None:intergenic 35.0%
!!! TGTTTTGAGAGGAAGATTTC+AGG - chr2.1:70956567-70956586 MS.gene002470:intron 35.0%
!!! TTTTTCAAGGATTGATGGCA+AGG + chr2.1:70956324-70956343 None:intergenic 35.0%
AACACGTATCTGATTGTGCA+GGG - chr2.1:70957004-70957023 MS.gene002470:intron 40.0%
AAGGAGCCCAAATAAAAGAG+CGG + chr2.1:70956857-70956876 None:intergenic 40.0%
ACAAGGATTTCCAATGAAGC+AGG - chr2.1:70954784-70954803 MS.gene002470:intron 40.0%
ACGTCTTCAAAATCACTGGT+GGG - chr2.1:70954753-70954772 MS.gene002470:intron 40.0%
ATATCACTGCTACGTAGCTT+TGG - chr2.1:70956042-70956061 MS.gene002470:intron 40.0%
CAAGGATTTCCAATGAAGCA+GGG - chr2.1:70954785-70954804 MS.gene002470:intron 40.0%
CAAGTCTATGAAAGCAGGAA+AGG + chr2.1:70956876-70956895 None:intergenic 40.0%
CACAACAACTGAATTGCAAC+AGG - chr2.1:70956674-70956693 MS.gene002470:intron 40.0%
GCCATCATCACCAACAAAAA+TGG + chr2.1:70954949-70954968 None:intergenic 40.0%
GGAAAGTAGTTTAGGAATCG+CGG - chr2.1:70956520-70956539 MS.gene002470:intron 40.0%
GGAAGAAACTTTACCTGCTT+TGG + chr2.1:70956009-70956028 None:intergenic 40.0%
GGATACGTCTTCAAAATCAC+TGG - chr2.1:70954749-70954768 MS.gene002470:intron 40.0%
GTAGAAAGGTTATACCTCTG+TGG + chr2.1:70954844-70954863 None:intergenic 40.0%
GTCATAAGTTAGTTAGCAGC+AGG + chr2.1:70956805-70956824 None:intergenic 40.0%
GTTGAAAAGCCAAGAATGAG+GGG - chr2.1:70955888-70955907 MS.gene002470:intron 40.0%
GTTTGGCTAAGAAGAGATCA+AGG - chr2.1:70957205-70957224 MS.gene002470:intron 40.0%
TAACACGTATCTGATTGTGC+AGG - chr2.1:70957003-70957022 MS.gene002470:intron 40.0%
TAACCAAGTTCAGAACAGAG+AGG + chr2.1:70955827-70955846 None:intergenic 40.0%
TACGTCTTCAAAATCACTGG+TGG - chr2.1:70954752-70954771 MS.gene002470:intron 40.0%
TAGCCCATCTAAATTGGAAC+CGG + chr2.1:70955020-70955039 None:intergenic 40.0%
TCATAGACTTGCATGATAGG+AGG - chr2.1:70956885-70956904 MS.gene002470:intron 40.0%
TCATGCAAGTCTATGAAAGC+AGG + chr2.1:70956881-70956900 None:intergenic 40.0%
TCTCCGGTTCCAATTTAGAT+GGG - chr2.1:70955014-70955033 MS.gene002470:intron 40.0%
TGCTACTCGCAGGAATTTAA+GGG - chr2.1:70954728-70954747 MS.gene002470:intron 40.0%
TTGCTACTCGCAGGAATTTA+AGG - chr2.1:70954727-70954746 MS.gene002470:intron 40.0%
! AAGGGTGAAAATGATTTGCC+AGG - chr2.1:70955855-70955874 MS.gene002470:intron 40.0%
! AGGATCTCACAAGAGGTTTT+GGG - chr2.1:70954611-70954630 MS.gene002470:intron 40.0%
! ATCTGGAGGCAAGAAGTTTT+TGG + chr2.1:70957155-70957174 None:intergenic 40.0%
! GTGTAGCAAGACTGTACTTT+TGG - chr2.1:70956285-70956304 MS.gene002470:intron 40.0%
! TTATTCGTTTCCTGGTTTGC+TGG - chr2.1:70955150-70955169 MS.gene002470:intron 40.0%
! TTGGCAGCTGACAAATTTTG+GGG + chr2.1:70955405-70955424 None:intergenic 40.0%
! TTTCCAAGGATGATGATGTC+AGG - chr2.1:70955949-70955968 MS.gene002470:intron 40.0%
!! CATCGATTTCAAGCTTCTTC+TGG + chr2.1:70954463-70954482 None:intergenic 40.0%
!! TATTCGTTTCCTGGTTTGCT+GGG - chr2.1:70955151-70955170 MS.gene002470:intron 40.0%
!! TTTGTTGGTGATGATGGCTT+GGG - chr2.1:70954951-70954970 MS.gene002470:intron 40.0%
!!! AGGTTTTAAGTTGAGTGGGA+AGG + chr2.1:70956391-70956410 None:intergenic 40.0%
!!! CCCATTTTTGTTGGTGATGA+TGG - chr2.1:70954945-70954964 MS.gene002470:intron 40.0%
!!! GGTTTTAAGTTGAGTGGGAA+GGG + chr2.1:70956390-70956409 None:intergenic 40.0%
!!! TTTTGTTGGTGATGATGGCT+TGG - chr2.1:70954950-70954969 MS.gene002470:intron 40.0%
AACGTTGCAAATCCAACCAC+TGG - chr2.1:70954434-70954453 MS.gene002470:CDS 45.0%
AAGTTCAGAACAGAGAGGTC+TGG + chr2.1:70955822-70955841 None:intergenic 45.0%
ACTTCTTGCCTCCAGATTGA+AGG - chr2.1:70957158-70957177 MS.gene002470:intron 45.0%
AGAAGAAGGAAGTCTGTTCG+TGG - chr2.1:70955783-70955802 MS.gene002470:CDS 45.0%
AGATTCAAACCGAAACCTCG+AGG + chr2.1:70954995-70955014 None:intergenic 45.0%
AGTATGTCAACACCTACCGT+CGG - chr2.1:70955973-70955992 MS.gene002470:intron 45.0%
AGTTCAGAACAGAGAGGTCT+GGG + chr2.1:70955821-70955840 None:intergenic 45.0%
CACTAATAGGAGACCCAAAC+TGG - chr2.1:70955210-70955229 MS.gene002470:intron 45.0%
CGATTAACACGTCAAAGGAG+AGG + chr2.1:70955592-70955611 None:intergenic 45.0%
CTCCAGATTGAAGGAGCAAA+GGG - chr2.1:70957167-70957186 MS.gene002470:intron 45.0%
CTCTCCGGTTCCAATTTAGA+TGG - chr2.1:70955013-70955032 MS.gene002470:intron 45.0%
CTTCCTGACATCATCATCCT+TGG + chr2.1:70955955-70955974 None:intergenic 45.0%
CTTTGGTTGTGAAAGTCCGA+CGG + chr2.1:70955992-70956011 None:intergenic 45.0%
GAAGAAGGAAGTCTGTTCGT+GGG - chr2.1:70955784-70955803 MS.gene002470:CDS 45.0%
GAATCGTCTACCATTAGGGA+TGG + chr2.1:70955652-70955671 None:intergenic 45.0%
GCAGCAGGAAATTAGTTGAG+TGG + chr2.1:70956790-70956809 None:intergenic 45.0%
GTCAGCGATTAACACGTCAA+AGG + chr2.1:70955597-70955616 None:intergenic 45.0%
GTTAACACACCCTGCTTCAT+TGG + chr2.1:70954797-70954816 None:intergenic 45.0%
TAACACGTCAAAGGAGAGGA+GGG + chr2.1:70955588-70955607 None:intergenic 45.0%
TACCATTAGGGATGGCAAAG+TGG + chr2.1:70955644-70955663 None:intergenic 45.0%
TCTTAGCCAAACTCTCACTG+CGG + chr2.1:70957197-70957216 None:intergenic 45.0%
TTAACACGTCAAAGGAGAGG+AGG + chr2.1:70955589-70955608 None:intergenic 45.0%
TTATACCTCTGTGGAGCAAG+AGG + chr2.1:70954835-70954854 None:intergenic 45.0%
TTCCCTTTGCTCCTTCAATC+TGG + chr2.1:70957172-70957191 None:intergenic 45.0%
! AGACCTCTCTGTTCTGAACT+TGG - chr2.1:70955821-70955840 MS.gene002470:CDS 45.0%
! AGGTTGCCTTGTTCTATCAG+AGG - chr2.1:70956905-70956924 MS.gene002470:intron 45.0%
! GAGGATCTCACAAGAGGTTT+TGG - chr2.1:70954610-70954629 MS.gene002470:intron 45.0%
! GGTTTCGGTTTGAATCTCTC+CGG - chr2.1:70954998-70955017 MS.gene002470:intron 45.0%
! TGCTGCCTCTGATTTTGCTT+TGG + chr2.1:70957127-70957146 None:intergenic 45.0%
!! CGGATTTTGGAAGCTCTCTT+AGG + chr2.1:70955915-70955934 None:intergenic 45.0%
!!! GAGGTTTTGGGTGATGCTTT+AGG - chr2.1:70954623-70954642 MS.gene002470:intron 45.0%
AACACGTCAAAGGAGAGGAG+GGG + chr2.1:70955587-70955606 None:intergenic 50.0%
AAGCTTCTTCTGGCAACCAG+TGG + chr2.1:70954453-70954472 None:intergenic 50.0%
CCTCCAGATTGAAGGAGCAA+AGG - chr2.1:70957166-70957185 MS.gene002470:intron 50.0%
CCTTTGCTCCTTCAATCTGG+AGG + chr2.1:70957169-70957188 None:intergenic 50.0%
CGGACTTTCACAACCAAAGC+AGG - chr2.1:70955993-70956012 MS.gene002470:intron 50.0%
CTCTTAGGACCCCTCATTCT+TGG + chr2.1:70955900-70955919 None:intergenic 50.0%
GAAGAGTCAAAGGAGTGACC+AGG + chr2.1:70957062-70957081 None:intergenic 50.0%
GAGTGGGAAGGGATAGAAGA+CGG + chr2.1:70956379-70956398 None:intergenic 50.0%
GGACAAGAGGATCTCACAAG+AGG - chr2.1:70954604-70954623 MS.gene002470:intron 50.0%
TGACCTGGCCTAAACATGAC+AGG - chr2.1:70955613-70955632 MS.gene002470:CDS 50.0%
TGACGTGTTAATCGCTGACC+TGG - chr2.1:70955598-70955617 MS.gene002470:CDS 50.0%
TGCTGCAACACCTTGCTTAG+CGG + chr2.1:70957238-70957257 None:intergenic 50.0%
TTCAAACCGAAACCTCGAGG+AGG + chr2.1:70954992-70955011 None:intergenic 50.0%
TTCTTCTGGCAACCAGTGGT+TGG + chr2.1:70954449-70954468 None:intergenic 50.0%
! ACTCCTTGTTTCCGAGGACA+CGG - chr2.1:70955744-70955763 MS.gene002470:CDS 50.0%
! ACTGGTGGGTGTGACAAACA+AGG - chr2.1:70954767-70954786 MS.gene002470:intron 50.0%
! GGCGTAATGGAGAGAGAAGA+AGG - chr2.1:70955769-70955788 MS.gene002470:CDS 50.0%
! TGACCAGGCGCTGTATCTTT+GGG + chr2.1:70957047-70957066 None:intergenic 50.0%
! TGCGTGATTTTGCTACTCGC+AGG - chr2.1:70954718-70954737 MS.gene002470:intron 50.0%
!! GTGATGATGGCTTGGGAACT+TGG - chr2.1:70954958-70954977 MS.gene002470:intron 50.0%
!!! GTCGTGCATTTTGGGACAAG+AGG - chr2.1:70954591-70954610 MS.gene002470:intron 50.0%
AAGCAGGGTGTGTTAACCCC+TGG - chr2.1:70954800-70954819 MS.gene002470:intron 55.0%
AATACCAGCGAGAATGGGGC+TGG - chr2.1:70955529-70955548 MS.gene002470:intron 55.0%
AGGCCTGTCATGTTTAGGCC+AGG + chr2.1:70955619-70955638 None:intergenic 55.0%
AGTGCTCCTCCTCGAGGTTT+CGG - chr2.1:70954983-70955002 MS.gene002470:intron 55.0%
CATTACGCCTTCCGTGTCCT+CGG + chr2.1:70955758-70955777 None:intergenic 55.0%
CTTCCGTGTCCTCGGAAACA+AGG + chr2.1:70955750-70955769 None:intergenic 55.0%
CTTGTTTCCGAGGACACGGA+AGG - chr2.1:70955748-70955767 MS.gene002470:CDS 55.0%
GGTTGTGAAAGTCCGACGGT+AGG + chr2.1:70955988-70956007 None:intergenic 55.0%
TGCAACACCTTGCTTAGCGG+TGG + chr2.1:70957235-70957254 None:intergenic 55.0%
TGGCCAGGCCTGTCATGTTT+AGG + chr2.1:70955624-70955643 None:intergenic 55.0%
TGGCCTAAACATGACAGGCC+TGG - chr2.1:70955618-70955637 MS.gene002470:CDS 55.0%
TTAGGGATGGCAAAGTGGCC+AGG + chr2.1:70955639-70955658 None:intergenic 55.0%
! GACCAGGCGCTGTATCTTTG+GGG + chr2.1:70957046-70957065 None:intergenic 55.0%
! GCAGGTACTCCTTGTTTCCG+AGG - chr2.1:70955738-70955757 MS.gene002470:CDS 55.0%
! GGCCACTTTGCCATCCCTAA+TGG - chr2.1:70955639-70955658 MS.gene002470:CDS 55.0%
! GTGACCAGGCGCTGTATCTT+TGG + chr2.1:70957048-70957067 None:intergenic 55.0%
AGCAAGAGGCGAACACGACC+AGG + chr2.1:70954821-70954840 None:intergenic 60.0%
AGCCCCAAAGATACAGCGCC+TGG - chr2.1:70957041-70957060 MS.gene002470:intron 60.0%
AGGCTCTCCACCGCTAAGCA+AGG - chr2.1:70957225-70957244 MS.gene002470:intron 60.0%
CAAGAGGCGAACACGACCAG+GGG + chr2.1:70954819-70954838 None:intergenic 60.0%
GCAAGAGGCGAACACGACCA+GGG + chr2.1:70954820-70954839 None:intergenic 60.0%
GGAACGCCGCAGTGAGAGTT+TGG - chr2.1:70957188-70957207 MS.gene002470:intron 60.0%
GTCAAAGGAGAGGAGGGGCA+GGG + chr2.1:70955582-70955601 None:intergenic 60.0%
GTTCGCCTCTTGCTCCACAG+AGG - chr2.1:70954827-70954846 MS.gene002470:intron 60.0%
! CGAGGACACGGAAGGCGTAA+TGG - chr2.1:70955756-70955775 MS.gene002470:CDS 60.0%
!! TCCTGGTTTGCTGGGCTGTC+CGG - chr2.1:70955159-70955178 MS.gene002470:intron 60.0%
!! TTGGAGAGTGCTCCTCCTCG+AGG - chr2.1:70954977-70954996 MS.gene002470:intron 60.0%
ACCGGACAGCCCAGCAAACC+AGG + chr2.1:70955163-70955182 None:intergenic 65.0%
CGTCAAAGGAGAGGAGGGGC+AGG + chr2.1:70955583-70955602 None:intergenic 65.0%
GTCACCAGCCCCATTCTCGC+TGG + chr2.1:70955536-70955555 None:intergenic 65.0%
AGGAGGGGCAGGGCAACGTG+AGG + chr2.1:70955572-70955591 None:intergenic 70.0%
AGGGGCAGGGCAACGTGAGG+CGG + chr2.1:70955569-70955588 None:intergenic 70.0%
GGGCAGGGCAACGTGAGGCG+GGG + chr2.1:70955567-70955586 None:intergenic 75.0%
GGGGCAGGGCAACGTGAGGC+GGG + chr2.1:70955568-70955587 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 70954343 70957260 70954343 ID=MS.gene002470
chr2.1 mRNA 70954343 70957260 70954343 ID=MS.gene002470.t1;Parent=MS.gene002470
chr2.1 exon 70957255 70957260 70957255 ID=MS.gene002470.t1.exon1;Parent=MS.gene002470.t1
chr2.1 CDS 70957255 70957260 70957255 ID=cds.MS.gene002470.t1;Parent=MS.gene002470.t1
chr2.1 exon 70957108 70957172 70957108 ID=MS.gene002470.t1.exon2;Parent=MS.gene002470.t1
chr2.1 CDS 70957108 70957172 70957108 ID=cds.MS.gene002470.t1;Parent=MS.gene002470.t1
chr2.1 exon 70956954 70957011 70956954 ID=MS.gene002470.t1.exon3;Parent=MS.gene002470.t1
chr2.1 CDS 70956954 70957011 70956954 ID=cds.MS.gene002470.t1;Parent=MS.gene002470.t1
chr2.1 exon 70956755 70956863 70956755 ID=MS.gene002470.t1.exon4;Parent=MS.gene002470.t1
chr2.1 CDS 70956755 70956863 70956755 ID=cds.MS.gene002470.t1;Parent=MS.gene002470.t1
chr2.1 exon 70955589 70955861 70955589 ID=MS.gene002470.t1.exon5;Parent=MS.gene002470.t1
chr2.1 CDS 70955589 70955861 70955589 ID=cds.MS.gene002470.t1;Parent=MS.gene002470.t1
chr2.1 exon 70954343 70954578 70954343 ID=MS.gene002470.t1.exon6;Parent=MS.gene002470.t1
chr2.1 CDS 70954343 70954578 70954343 ID=cds.MS.gene002470.t1;Parent=MS.gene002470.t1
Gene Sequence

>MS.gene002470

ATGAAGGTTACTCTCTCAAACCGTAATTTCCCTTGTTCCTTTGATTTCATTGCTATTATGACTAAACCATATAGAAATTATTTTGCAGTTCAACGTTGCAAATCCAACCACTGGTTGCCAGAAGAAGCTTGAAATCGATGATGACATGAAGCTGTAATCTCTTCACCTTTTTCAATTTTTATTGCCTTTCTTCAATTCACACGATTTATTCTCATGTTCCTAATTTTTATTTTTATTTTTAAATTGTAGTCGTGCATTTTGGGACAAGAGGATCTCACAAGAGGTTTTGGGTGATGCTTTAGGCGAAGTATGTTTGAATCTAGAAAAATTATTTGAATAATCCTTAAATTAATTGTTATATTGATTTATAACTGTTGCGTGATTTTGCTACTCGCAGGAATTTAAGGGATACGTCTTCAAAATCACTGGTGGGTGTGACAAACAAGGATTTCCAATGAAGCAGGGTGTGTTAACCCCTGGTCGTGTTCGCCTCTTGCTCCACAGAGGTATAACCTTTCTACAGTTTTTTTTTTTTTTTTTGTGTTTTTATTAATTATTATAGTGAGGTTAATGATTATACTTAAAATTATATGTACTATAAACCCATTTTTGTTGGTGATGATGGCTTGGGAACTTGGAGAGTGCTCCTCCTCGAGGTTTCGGTTTGAATCTCTCCGGTTCCAATTTAGATGGGCTAATTTAGCTTCTTCAAAAGAAAAATTATATGTACTATTGTTTGCGTTATAGGTGTTGTATACTCTTTATCATTTACTTGAATGGTTCCTTTTGTTTTTATTACTGTTTGAATTATTCGTTTCCTGGTTTGCTGGGCTGTCCGGTTTAACCCTCTATTTTAGATCTTTTTTTCACTAATAGGAGACCCAAACTGGTGAACTTATTTTGTATGTTGGTGAATTTTTTTTGTAGGCATTTCATGCTTGCTTGTTGTTGTTGCTGTTAATTATTCCTTTTCAGTTTTGTTAATTATGCTGTTTTATTGTTCATGATGTTTGATTTATTAATTATGTTGTTCGGTGGCATTGAGCCATTGATGTTTTTCCCCAAAATTTGTCAGCTGCCAATTTATACATACATATATACATATACATGTATATATATACATATATATATATATATATATATATATATATATATATATATATATATGCAGCAATATGTTTTTTAAAATACCAGCGAGAATGGGGCTGGTGACTACTTGAGCCCCGCCTCACGTTGCCCTGCCCCTCCTCTCCTTTGACGTGTTAATCGCTGACCTGGCCTAAACATGACAGGCCTGGCCACTTTGCCATCCCTAATGGTAGACGATTCTCTTAGCAAAGTAGCAAACCCTTGTTAAGTATTTATTTGGTAAGTTTTAATTTAATGTTTGTTTGAGCAGGTACTCCTTGTTTCCGAGGACACGGAAGGCGTAATGGAGAGAGAAGAAGGAAGTCTGTTCGTGGGTGCATTGTCAGCCCAGACCTCTCTGTTCTGAACTTGGTTATTGTGAAGAAGGGTGAAAATGATTTGCCAGGTTTGACTGATGTTGAAAAGCCAAGAATGAGGGGTCCTAAGAGAGCTTCCAAAATCCGTAAGCTCTTCAATCTTTCCAAGGATGATGATGTCAGGAAGTATGTCAACACCTACCGTCGGACTTTCACAACCAAAGCAGGTAAAGTTTCTTCCTGATTATCTGTGAATATCACTGCTACGTAGCTTTGGTTTTGAAAAATGTTCTTGGAAAATATCCTATTTAGTATTTATCACTTGTAATATATTGTCTCCAATATCTAAGTTGGAATTTTTCAAAATCACTTGCTATTATAATATTGGGTGAATCTGGTCGGTGAAACTTTACTGTTAAACTTTAAACTTCCTGTATGTATTGTTAATGTCATATAAGAAATTATGCAATTCAGTTGCCCATTGTATAGATGAGTTTGTGTAGCAAGACTGTACTTTTGGAATAGTGTGTTTCCCTTGCCATCAATCCTTGAAAAATTGAAAGAAGCTAATACACACTGCATAAGAAGCCGTCTTCTATCCCTTCCCACTCAACTTAAAACCTTTTGTTTTGTATATTAATTATGCTATGTTATGGTTAGAAGTATAGGATGATAGAACAAAGTGACTGCTGTTATAAATGTCATTAGTTAGTTAACATTAGCAAGTTAGTTGGAAAGTAGTTTAGGAATCGCGGAGTGAATGCAAGAGAATATTTGGTTGTTTTGAGAGGAAGATTTCAGGTTTCTCGAATAACATGATAGATTGCTAAATAGATGAAGAGGGATATTCCCGTTGCTGTTAATTGGAACTAGATTAAAAAAAAGACACAACAACTGAATTGCAACAGGGATATCCCTCTGACTCTATTTTTAAATCTCCCAAAACAATTGCAAATTGACAAAACTGACTTCTCTTGCATACAATCTGCTATTGATAGACCACTCAACTAATTTCCTGCTGCTAACTAACTTATGACAATTATTACAACTGTCAGTTGCAACATATCCGCTCTTTTATTTGGGCTCCTTTCCTGCTTTCATAGACTTGCATGATAGGAGGTTGCCTTGTTCTATCAGAGGACTTATTTTCTAATAGAATTTTATCTAAGGTCATAACTATGGATCTATAATTTATAATGGTTTCTAGTCGCAAAGTAACACGTATCTGATTGTGCAGGGAAAAAGCAGAGCAAAGCCCCAAAGATACAGCGCCTGGTCACTCCTTTGACTCTTCAAAGAAAGAGGGCAAGAATTGCTGATAAGAAGAAAAGAATTGCCAAAGCAAAATCAGAGGCAGCAGATTACCAAAAACTTCTTGCCTCCAGATTGAAGGAGCAAAGGGAACGCCGCAGTGAGAGTTTGGCTAAGAAGAGATCAAGGCTCTCCACCGCTAAGCAAGGTGTTGCAGCATAG
Protein sequence

>MS.gene002470.t1

MKFNVANPTTGCQKKLEIDDDMKLRAFWDKRISQEVLGDALGEEFKGYVFKITGGCDKQGFPMKQGVLTPGRVRLLLHRGTPCFRGHGRRNGERRRKSVRGCIVSPDLSVLNLVIVKKGENDLPGLTDVEKPRMRGPKRASKIRKLFNLSKDDDVRKYVNTYRRTFTTKAGKKQSKAPKIQRLVTPLTLQRKRARIADKKKRIAKAKSEAADYQKLLASRLKEQRERRSESLAKKRSRLSTAKQGVAA