AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene09046

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09046.t1 MTR_8g080150 95.787 356 11 2 1 355 1 353 0.0 692
MS.gene09046.t1 MTR_8g080140 68.661 351 102 4 9 355 7 353 3.21e-170 479
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09046.t1 AT5G11010 55.908 347 145 4 12 355 10 351 2.67e-136 392
MS.gene09046.t1 AT5G11010 55.908 347 145 4 12 355 10 351 2.67e-136 392
MS.gene09046.t1 AT5G11010 55.908 347 145 4 12 355 10 351 2.67e-136 392
MS.gene09046.t1 AT5G11010 54.426 305 131 4 12 313 10 309 1.84e-115 338

Find 79 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAATAAACTTGTAGGAATT+TGG 0.140890 4.3:-16644304 None:intergenic
AATACCTCTGGCTGGGTTAA+AGG 0.251617 4.3:+16642565 MS.gene09046:CDS
CTTACCTGCAATAAACTTGT+AGG 0.273363 4.3:-16644311 None:intergenic
CATTTCAGGTGATGGTTATA+AGG 0.285128 4.3:+16642692 MS.gene09046:intron
AAAAGTCAATCTCTTGCTTC+AGG 0.287578 4.3:+16644274 MS.gene09046:CDS
AATTTACCTGTTGGAAAGTT+CGG 0.321432 4.3:+16642786 MS.gene09046:CDS
AGCCTGAATGCTAGTATTGT+TGG 0.330336 4.3:+16643288 MS.gene09046:CDS
CTTGCGATGGGAACATTATA+AGG 0.336390 4.3:-16643125 None:intergenic
CTGATTACACCTGTGCCATT+TGG 0.342406 4.3:+16644242 MS.gene09046:CDS
CTCGGTCGCATCTAGTTAAT+AGG 0.357472 4.3:-16641200 None:intergenic
CTGGAGAGTATTTCAGAGAT+TGG 0.360131 4.3:-16643267 None:intergenic
CCTCCCATCACATTTGTTTG+TGG 0.372621 4.3:+16641129 MS.gene09046:CDS
AATTCCTACAAGTTTATTGC+AGG 0.374142 4.3:+16644307 MS.gene09046:CDS
CAACCTGAGTTTACTCCTCC+TGG 0.374377 4.3:+16641249 MS.gene09046:CDS
ATTTACCTGTTGGAAAGTTC+GGG 0.375937 4.3:+16642787 MS.gene09046:CDS
CCCCAGGGTGCCTGCTCAAA+AGG 0.383947 4.3:+16642989 MS.gene09046:intron
AGGGGCATTGACACAGTTAA+AGG 0.386318 4.3:+16644209 MS.gene09046:CDS
GAAAGAGCAATGGGTTCATC+TGG 0.388190 4.3:+16641033 None:intergenic
TTACCCTGGTGCCTTGGTCT+TGG 0.389061 4.3:+16643342 MS.gene09046:CDS
AGCCAACAATACTAGCATTC+AGG 0.390103 4.3:-16643290 None:intergenic
GTTGGAAAGTTCGGGTCAAA+TGG 0.391820 4.3:+16642795 MS.gene09046:CDS
TTGTAGTGAATACCTCTGGC+TGG 0.403286 4.3:+16642557 MS.gene09046:CDS
GTGAACATGCTAAAATATAT+TGG 0.405008 4.3:+16642720 MS.gene09046:CDS
TATTTATGATTATTATCATA+AGG 0.411756 4.3:+16642483 MS.gene09046:CDS
TTTCATAGTTTATACCTCTC+TGG 0.415607 4.3:-16641764 None:intergenic
TTCAGATATTTCAACAATCA+AGG 0.444136 4.3:+16643073 MS.gene09046:CDS
TGGAGAGTATTTCAGAGATT+GGG 0.453316 4.3:-16643266 None:intergenic
CCCACTCATATAGTTAAGAT+AGG 0.455843 4.3:+16642744 MS.gene09046:CDS
TGAGATATATCCAGCTCGTC+AGG 0.461392 4.3:+16642845 MS.gene09046:CDS
TTTAATGAGTCCTGACGAGC+TGG 0.479415 4.3:-16642855 None:intergenic
CAGCTTGTGACCATTGTTGT+GGG 0.481609 4.3:-16641080 None:intergenic
AGGAGTTGGACTGGATGTAA+TGG 0.489408 4.3:-16641109 None:intergenic
AAAGTCAATCTCTTGCTTCA+GGG 0.496620 4.3:+16644275 MS.gene09046:CDS
ACAAATGTGATGGGAGGAGT+TGG 0.500253 4.3:-16641123 None:intergenic
TTTGACCCGAACTTTCCAAC+AGG 0.502390 4.3:-16642792 None:intergenic
GCAGCTTGTGACCATTGTTG+TGG 0.508024 4.3:-16641081 None:intergenic
TGTTGTGGGATGTAAATGTC+TGG 0.513328 4.3:-16641066 None:intergenic
CCTCTTGTAGTGAATACCTC+TGG 0.514438 4.3:+16642553 MS.gene09046:CDS
TTCAGAGAACCAAATGGCAC+AGG 0.515584 4.3:-16644251 None:intergenic
TTGTGATTCAGGTATTGTGA+GGG 0.527148 4.3:+16644190 MS.gene09046:intron
CCTACCAAGACCAAGGCACC+AGG 0.529524 4.3:-16643346 None:intergenic
TCGAATCTTTATGCTTGCGA+TGG 0.529810 4.3:-16643138 None:intergenic
GCTTATCTTGAAACTGATGT+TGG 0.537454 4.3:+16641225 MS.gene09046:CDS
TGTAGTGAATACCTCTGGCT+GGG 0.538425 4.3:+16642558 MS.gene09046:CDS
TGCTCCACAAACAAATGTGA+TGG 0.541521 4.3:-16641133 None:intergenic
TTGGAAAGTTCGGGTCAAAT+GGG 0.542587 4.3:+16642796 MS.gene09046:CDS
ATTGTTAATAAAGTTACTCC+AGG 0.544370 4.3:+16641288 MS.gene09046:CDS
AACAATACTAGCATTCAGGC+TGG 0.547070 4.3:-16643286 None:intergenic
CTTGTGATTCAGGTATTGTG+AGG 0.553596 4.3:+16644189 MS.gene09046:intron
GATCTGAAGAATTTACCTGT+TGG 0.554922 4.3:+16642777 MS.gene09046:CDS
CGAATCTTTATGCTTGCGAT+GGG 0.557731 4.3:-16643137 None:intergenic
GGCTTAGCAGTTGAATCTGA+AGG 0.565104 4.3:+16643309 MS.gene09046:CDS
ATAAGGACAGTGAGAGGTCA+AGG 0.568437 4.3:-16643108 None:intergenic
ATTGAGGAGATTTCGCGAGA+AGG 0.573264 4.3:-16641175 None:intergenic
TGTGATTCAGGTATTGTGAG+GGG 0.578885 4.3:+16644191 MS.gene09046:intron
TGGAGTAACTTTATTAACAA+TGG 0.579021 4.3:-16641286 None:intergenic
AGGTAAATTCTTCAGATCAG+TGG 0.580946 4.3:-16642772 None:intergenic
GCCTATCTTAACTATATGAG+TGG 0.583863 4.3:-16642745 None:intergenic
TGTGATGGGAGGAGTTGGAC+TGG 0.596371 4.3:-16641118 None:intergenic
CCAGAGGTATTCACTACAAG+AGG 0.598694 4.3:-16642553 None:intergenic
CAGGAGGAGTAAACTCAGGT+TGG 0.603274 4.3:-16641248 None:intergenic
ATGGTAAGAGAGATAAAACC+AGG 0.613877 4.3:-16641267 None:intergenic
GCTCCACAAACAAATGTGAT+GGG 0.614241 4.3:-16641132 None:intergenic
ATCTAGTTAATAGGACATTG+AGG 0.620697 4.3:-16641191 None:intergenic
CAGTTTCAAGATAAGCAACT+CGG 0.625840 4.3:-16641218 None:intergenic
CCTATCTTAACTATATGAGT+GGG 0.628881 4.3:-16642744 None:intergenic
GTTGAAATATCTGAATCACT+AGG 0.636570 4.3:-16643065 None:intergenic
TTTACCTTTAACCCAGCCAG+AGG 0.641811 4.3:-16642569 None:intergenic
AAACCAGGAGGAGTAAACTC+AGG 0.646510 4.3:-16641252 None:intergenic
AACATTATAAGGACAGTGAG+AGG 0.648592 4.3:-16643114 None:intergenic
GATTCGACGTGTTCTTCACG+AGG 0.658346 4.3:+16643154 MS.gene09046:CDS
GTGGGATGTAAATGTCTGGA+AGG 0.660248 4.3:-16641062 None:intergenic
ACATTTACATCCCACAACAA+TGG 0.661470 4.3:+16641070 MS.gene09046:CDS
CCACAAACAAATGTGATGGG+AGG 0.666150 4.3:-16641129 None:intergenic
GTTTGTGGAGCAAGTAACTG+CGG 0.670641 4.3:+16641144 MS.gene09046:CDS
CAAGTTACCTACCAAGACCA+AGG 0.684244 4.3:-16643353 None:intergenic
CTACCAAGACCAAGGCACCA+GGG 0.712305 4.3:-16643345 None:intergenic
GTAAGAGAGATAAAACCAGG+AGG 0.735353 4.3:-16641264 None:intergenic
CATGCCTGAAAACACCAGAG+AGG 0.772404 4.3:+16641750 MS.gene09046:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATTGATATATTGTTAT+TGG - chr4.3:16642643-16642662 None:intergenic 10.0%
!! TATTTATGATTATTATCATA+AGG + chr4.3:16642483-16642502 MS.gene09046:CDS 10.0%
!! ATTATTGAATCAATGAATAT+AGG - chr4.3:16641654-16641673 None:intergenic 15.0%
!! TGAATAAAGTTACAAAATAA+AGG + chr4.3:16643594-16643613 MS.gene09046:intron 15.0%
!! TTATTGAATCAATGAATATA+GGG - chr4.3:16641653-16641672 None:intergenic 15.0%
!! TTCTATAAAAATGTTCATTA+TGG + chr4.3:16641675-16641694 MS.gene09046:intron 15.0%
!! ATATCTCATCTCAATAATTT+GGG + chr4.3:16642161-16642180 MS.gene09046:intron 20.0%
!! TGTAAACAAAAAACAACATT+TGG + chr4.3:16643821-16643840 MS.gene09046:intron 20.0%
!! TTAACAAGATACAATAGTTA+AGG - chr4.3:16641911-16641930 None:intergenic 20.0%
!! TTACATGTAAATGTTAGAAT+AGG - chr4.3:16641332-16641351 None:intergenic 20.0%
!!! AAATGTTGTTTTTTGTTTAC+AGG - chr4.3:16643822-16643841 None:intergenic 20.0%
!!! ATATTTGATTCTTTTAGTGT+CGG + chr4.3:16642312-16642331 MS.gene09046:intron 20.0%
!!! ATGTATTTTAAGTATGTTGA+CGG - chr4.3:16642457-16642476 None:intergenic 20.0%
!!! ATTATATCTCATATTTCTGT+TGG + chr4.3:16643946-16643965 MS.gene09046:intron 20.0%
!!! TGACTTATGAATATTTTTGT+AGG + chr4.3:16643242-16643261 MS.gene09046:intron 20.0%
!!! TTCTTTATCTTTTCTATTTG+TGG - chr4.3:16644040-16644059 None:intergenic 20.0%
! AAGAAACATCACCAAAAAAA+AGG - chr4.3:16642423-16642442 None:intergenic 25.0%
! AGAAACATCACCAAAAAAAA+GGG - chr4.3:16642422-16642441 None:intergenic 25.0%
! AGAATAAATTCATATTCCTC+TGG + chr4.3:16644056-16644075 MS.gene09046:intron 25.0%
! ATTCATAGAGTTGAATCATT+TGG - chr4.3:16643579-16643598 None:intergenic 25.0%
! ATTGTTAATAAAGTTACTCC+AGG + chr4.3:16641288-16641307 MS.gene09046:CDS 25.0%
! CTTGATATCATTCAAACTTA+TGG + chr4.3:16643876-16643895 MS.gene09046:intron 25.0%
! GATATCTCATCTCAATAATT+TGG + chr4.3:16642160-16642179 MS.gene09046:intron 25.0%
! GGATGTTTGATACATTAAAA+AGG - chr4.3:16644019-16644038 None:intergenic 25.0%
! GTGAACATGCTAAAATATAT+TGG + chr4.3:16642720-16642739 MS.gene09046:CDS 25.0%
! TAAAAGGAATTTGCATTTGT+AGG + chr4.3:16643915-16643934 MS.gene09046:intron 25.0%
! TGGAGTAACTTTATTAACAA+TGG - chr4.3:16641289-16641308 None:intergenic 25.0%
! TTAATGACATTGATACAAAC+TGG - chr4.3:16642120-16642139 None:intergenic 25.0%
! TTCAGATATTTCAACAATCA+AGG + chr4.3:16643073-16643092 MS.gene09046:CDS 25.0%
! TTCTTAATAGCATAGATTTG+AGG + chr4.3:16641993-16642012 MS.gene09046:intron 25.0%
!! AATTCTTTTATCTTTGTGAG+TGG - chr4.3:16642025-16642044 None:intergenic 25.0%
!! ACGAGATTTGATAATTATGT+TGG + chr4.3:16641609-16641628 MS.gene09046:intron 25.0%
!! AGAATTTTGTCTCTTATCAA+AGG - chr4.3:16642518-16642537 None:intergenic 25.0%
!! CGAGATTTGATAATTATGTT+GGG + chr4.3:16641610-16641629 MS.gene09046:intron 25.0%
!!! AAACTTGTCACTTTTTTCAT+GGG + chr4.3:16641578-16641597 MS.gene09046:intron 25.0%
!!! TATTTGTTGCTTTGAATTTG+AGG + chr4.3:16642209-16642228 MS.gene09046:intron 25.0%
AATACCTATTAATCAGCTTG+AGG - chr4.3:16643678-16643697 None:intergenic 30.0%
AATTTACCTGTTGGAAAGTT+CGG + chr4.3:16642786-16642805 MS.gene09046:CDS 30.0%
ACTAAACTATCAGCTGAAAA+TGG - chr4.3:16642910-16642929 None:intergenic 30.0%
AGATACAATAGTTAAGGTTG+AGG - chr4.3:16641905-16641924 None:intergenic 30.0%
AGGATATTAGTTACATACAC+AGG + chr4.3:16643614-16643633 MS.gene09046:intron 30.0%
ATACCTATTAATCAGCTTGA+GGG - chr4.3:16643677-16643696 None:intergenic 30.0%
ATCTAGTTAATAGGACATTG+AGG - chr4.3:16641194-16641213 None:intergenic 30.0%
ATGTCATTAACATGTGATAC+TGG + chr4.3:16642130-16642149 MS.gene09046:intron 30.0%
CACGTAGTAAAACTAAATGT+GGG - chr4.3:16641454-16641473 None:intergenic 30.0%
CAGAATATTGTATACAAGCT+GGG - chr4.3:16643708-16643727 None:intergenic 30.0%
CATTCAAATAAGACATGGAT+TGG - chr4.3:16642338-16642357 None:intergenic 30.0%
CCTATCTTAACTATATGAGT+GGG - chr4.3:16642747-16642766 None:intergenic 30.0%
CTTAGCATCATTACTGTTAA+AGG + chr4.3:16644155-16644174 MS.gene09046:intron 30.0%
GAGAAAAGCAGTAGTTTATT+TGG - chr4.3:16642618-16642637 None:intergenic 30.0%
GCAATAAACTTGTAGGAATT+TGG - chr4.3:16644307-16644326 None:intergenic 30.0%
GCATAACAATGTGACAAAAT+AGG - chr4.3:16642236-16642255 None:intergenic 30.0%
GCATTCATTCAAATAAGACA+TGG - chr4.3:16642343-16642362 None:intergenic 30.0%
GTTGAAATATCTGAATCACT+AGG - chr4.3:16643068-16643087 None:intergenic 30.0%
TATATGTTAGAAAACCTACC+TGG - chr4.3:16641309-16641328 None:intergenic 30.0%
TTAATAAAGTTACTCCAGGT+AGG + chr4.3:16641292-16641311 MS.gene09046:intron 30.0%
TTTCATAGTTTATACCTCTC+TGG - chr4.3:16641767-16641786 None:intergenic 30.0%
! TTTGTCTGTTTGTAGTTTGT+AGG + chr4.3:16642287-16642306 MS.gene09046:intron 30.0%
!! GTATACAATATTCTGGAACA+TGG + chr4.3:16643713-16643732 MS.gene09046:intron 30.0%
!! GTTTAAAATTTTCAAGCACG+TGG - chr4.3:16641552-16641571 None:intergenic 30.0%
!! TTCTGATCGAAAGTATCTTA+AGG - chr4.3:16642064-16642083 None:intergenic 30.0%
!!! ACTTTTTTCAGAGAACCAAA+TGG - chr4.3:16644260-16644279 None:intergenic 30.0%
!!! CAAACTTGTCACTTTTTTCA+TGG + chr4.3:16641577-16641596 MS.gene09046:intron 30.0%
!!! GTTTTGATTTCTTGTGATTC+AGG + chr4.3:16644179-16644198 MS.gene09046:intron 30.0%
AAAAGTCAATCTCTTGCTTC+AGG + chr4.3:16644274-16644293 MS.gene09046:CDS 35.0%
AAAGTCAATCTCTTGCTTCA+GGG + chr4.3:16644275-16644294 MS.gene09046:CDS 35.0%
AACATTATAAGGACAGTGAG+AGG - chr4.3:16643117-16643136 None:intergenic 35.0%
ACATTTACATCCCACAACAA+TGG + chr4.3:16641070-16641089 MS.gene09046:CDS 35.0%
AGGTAAATTCTTCAGATCAG+TGG - chr4.3:16642775-16642794 None:intergenic 35.0%
ATGACAGCCAGAAAATTAGA+TGG - chr4.3:16644121-16644140 None:intergenic 35.0%
ATGGTAAGAGAGATAAAACC+AGG - chr4.3:16641270-16641289 None:intergenic 35.0%
ATTCCAAAATGTCTCGTCAT+AGG + chr4.3:16643388-16643407 MS.gene09046:intron 35.0%
ATTTACCTGTTGGAAAGTTC+GGG + chr4.3:16642787-16642806 MS.gene09046:CDS 35.0%
CAGTTTCAAGATAAGCAACT+CGG - chr4.3:16641221-16641240 None:intergenic 35.0%
CATAGAGTTGAATCATTTGG+AGG - chr4.3:16643576-16643595 None:intergenic 35.0%
CATAGTTTATACATCTCTCG+TGG - chr4.3:16641869-16641888 None:intergenic 35.0%
CATGTACATAGAACACTCAT+TGG - chr4.3:16643469-16643488 None:intergenic 35.0%
CCAGAATATTGTATACAAGC+TGG - chr4.3:16643709-16643728 None:intergenic 35.0%
CCAGCTTGTATACAATATTC+TGG + chr4.3:16643706-16643725 MS.gene09046:intron 35.0%
CCCACTCATATAGTTAAGAT+AGG + chr4.3:16642744-16642763 MS.gene09046:CDS 35.0%
GATCTGAAGAATTTACCTGT+TGG + chr4.3:16642777-16642796 MS.gene09046:CDS 35.0%
GCACGTAGTAAAACTAAATG+TGG - chr4.3:16641455-16641474 None:intergenic 35.0%
GCCTATCTTAACTATATGAG+TGG - chr4.3:16642748-16642767 None:intergenic 35.0%
GCTTATCTTGAAACTGATGT+TGG + chr4.3:16641225-16641244 MS.gene09046:CDS 35.0%
TAATGTGGTCAACTAGTTGA+AGG + chr4.3:16643757-16643776 MS.gene09046:intron 35.0%
TACCTATTAATCAGCTTGAG+GGG - chr4.3:16643676-16643695 None:intergenic 35.0%
TAGGGAATCTCAAACATCAT+CGG - chr4.3:16643539-16643558 None:intergenic 35.0%
TAGTTTCTTGTTCTGCCTTA+GGG - chr4.3:16643495-16643514 None:intergenic 35.0%
TGAGTTGCAACACATCTTAA+TGG + chr4.3:16643986-16644005 MS.gene09046:intron 35.0%
TTAGTTTCTTGTTCTGCCTT+AGG - chr4.3:16643496-16643515 None:intergenic 35.0%
! ATATTTCTGTTGGCTGTAAC+AGG + chr4.3:16643956-16643975 MS.gene09046:intron 35.0%
! ATGCTACTTATCATCTCTTC+TGG + chr4.3:16643186-16643205 MS.gene09046:intron 35.0%
! CATACAGCCATCTAATTTTC+TGG + chr4.3:16644111-16644130 MS.gene09046:intron 35.0%
! CATTTCAGGTGATGGTTATA+AGG + chr4.3:16642692-16642711 MS.gene09046:intron 35.0%
! TCAATTCTTGACTTGACCAA+TGG + chr4.3:16641935-16641954 MS.gene09046:intron 35.0%
! TGTGTTTTGTCTTGCATTTC+AGG + chr4.3:16642678-16642697 MS.gene09046:intron 35.0%
! TTGTGATTCAGGTATTGTGA+GGG + chr4.3:16644190-16644209 MS.gene09046:intron 35.0%
!! TGGAGAGTATTTCAGAGATT+GGG - chr4.3:16643269-16643288 None:intergenic 35.0%
!!! CACTCATTGGTGTTTTGTTT+AGG - chr4.3:16643456-16643475 None:intergenic 35.0%
!!! CGTAACAGATCCCTTTTTTT+TGG + chr4.3:16642409-16642428 MS.gene09046:intron 35.0%
!!! TGACGAGACATTTTGGAATT+TGG - chr4.3:16643387-16643406 None:intergenic 35.0%
AACAATACTAGCATTCAGGC+TGG - chr4.3:16643289-16643308 None:intergenic 40.0%
AAGGACCTAAAAAGTTACCC+TGG + chr4.3:16643328-16643347 MS.gene09046:CDS 40.0%
ACCTATTAATCAGCTTGAGG+GGG - chr4.3:16643675-16643694 None:intergenic 40.0%
AGCCAACAATACTAGCATTC+AGG - chr4.3:16643293-16643312 None:intergenic 40.0%
AGCCTGAATGCTAGTATTGT+TGG + chr4.3:16643288-16643307 MS.gene09046:CDS 40.0%
ATATCCCTATTTGTTTCCCC+AGG + chr4.3:16642973-16642992 MS.gene09046:intron 40.0%
ATCTTGTAATGACGAGCACA+TGG + chr4.3:16644086-16644105 MS.gene09046:intron 40.0%
ATGAATGCGCTCACTTGTAA+TGG + chr4.3:16642355-16642374 MS.gene09046:intron 40.0%
CGAATCTTTATGCTTGCGAT+GGG - chr4.3:16643140-16643159 None:intergenic 40.0%
CTTGCGATGGGAACATTATA+AGG - chr4.3:16643128-16643147 None:intergenic 40.0%
GCTCCACAAACAAATGTGAT+GGG - chr4.3:16641135-16641154 None:intergenic 40.0%
GTAAGAGAGATAAAACCAGG+AGG - chr4.3:16641267-16641286 None:intergenic 40.0%
GTCTTCTACAAACAGTGAGT+TGG + chr4.3:16642257-16642276 MS.gene09046:intron 40.0%
TAGCAGTGAGGTACACTAAA+AGG + chr4.3:16643899-16643918 MS.gene09046:intron 40.0%
TATCCCTATTTGTTTCCCCA+GGG + chr4.3:16642974-16642993 MS.gene09046:intron 40.0%
TCAAACTTATGGTAGCAGTG+AGG + chr4.3:16643887-16643906 MS.gene09046:intron 40.0%
TCGAATCTTTATGCTTGCGA+TGG - chr4.3:16643141-16643160 None:intergenic 40.0%
TCTATGTACATGAGACCCTA+AGG + chr4.3:16643477-16643496 MS.gene09046:intron 40.0%
TGCTCCACAAACAAATGTGA+TGG - chr4.3:16641136-16641155 None:intergenic 40.0%
TGTTGTGGGATGTAAATGTC+TGG - chr4.3:16641069-16641088 None:intergenic 40.0%
TTACAAGATCAGAAGACCAG+AGG - chr4.3:16644075-16644094 None:intergenic 40.0%
TTGACCACATTACTGCCAAA+GGG - chr4.3:16643749-16643768 None:intergenic 40.0%
TTGGAAAGTTCGGGTCAAAT+GGG + chr4.3:16642796-16642815 MS.gene09046:CDS 40.0%
! ATTTGAAGCCTTCTACACGA+AGG - chr4.3:16643798-16643817 None:intergenic 40.0%
! CAACCTATGACGAGACATTT+TGG - chr4.3:16643394-16643413 None:intergenic 40.0%
! CTTGTGATTCAGGTATTGTG+AGG + chr4.3:16644189-16644208 MS.gene09046:intron 40.0%
! GTAATTGAGTCTGAGACCAT+TGG - chr4.3:16641954-16641973 None:intergenic 40.0%
! TATACCTCTCTGGTGTTTTC+AGG - chr4.3:16641757-16641776 None:intergenic 40.0%
! TGTGATTCAGGTATTGTGAG+GGG + chr4.3:16644191-16644210 MS.gene09046:intron 40.0%
! TTGTCTTGCATTTCAGGTGA+TGG + chr4.3:16642684-16642703 MS.gene09046:intron 40.0%
!! CTGGAGAGTATTTCAGAGAT+TGG - chr4.3:16643270-16643289 None:intergenic 40.0%
!!! CTTGTCACTTTTTTCATGGG+AGG + chr4.3:16641581-16641600 MS.gene09046:intron 40.0%
AAAAAGTTACCCTGGTGCCT+TGG + chr4.3:16643336-16643355 MS.gene09046:CDS 45.0%
AAACCAGGAGGAGTAAACTC+AGG - chr4.3:16641255-16641274 None:intergenic 45.0%
AATACCTCTGGCTGGGTTAA+AGG + chr4.3:16642565-16642584 MS.gene09046:CDS 45.0%
ATAAGGACAGTGAGAGGTCA+AGG - chr4.3:16643111-16643130 None:intergenic 45.0%
ATTGAGGAGATTTCGCGAGA+AGG - chr4.3:16641178-16641197 None:intergenic 45.0%
CAAGTTACCTACCAAGACCA+AGG - chr4.3:16643356-16643375 None:intergenic 45.0%
CAGCTTGTGACCATTGTTGT+GGG - chr4.3:16641083-16641102 None:intergenic 45.0%
CCTCCCATCACATTTGTTTG+TGG + chr4.3:16641129-16641148 MS.gene09046:CDS 45.0%
CCTCTTGTAGTGAATACCTC+TGG + chr4.3:16642553-16642572 MS.gene09046:CDS 45.0%
CTCGGTCGCATCTAGTTAAT+AGG - chr4.3:16641203-16641222 None:intergenic 45.0%
GGCTTAGCAGTTGAATCTGA+AGG + chr4.3:16643309-16643328 MS.gene09046:CDS 45.0%
GTGGGATGTAAATGTCTGGA+AGG - chr4.3:16641065-16641084 None:intergenic 45.0%
GTTGACCACATTACTGCCAA+AGG - chr4.3:16643750-16643769 None:intergenic 45.0%
GTTGGAAAGTTCGGGTCAAA+TGG + chr4.3:16642795-16642814 MS.gene09046:CDS 45.0%
GTTTGTGGAGCAAGTAACTG+CGG + chr4.3:16641144-16641163 MS.gene09046:CDS 45.0%
TACACGAAGGCCGCAAAATA+GGG - chr4.3:16643785-16643804 None:intergenic 45.0%
TCATTTGGAGGACTCGTTAG+TGG - chr4.3:16643564-16643583 None:intergenic 45.0%
TCCCCCTCAAGCTGATTAAT+AGG + chr4.3:16643671-16643690 MS.gene09046:intron 45.0%
TGAGATATATCCAGCTCGTC+AGG + chr4.3:16642845-16642864 MS.gene09046:CDS 45.0%
TGTAGTGAATACCTCTGGCT+GGG + chr4.3:16642558-16642577 MS.gene09046:CDS 45.0%
TTCAGAGAACCAAATGGCAC+AGG - chr4.3:16644254-16644273 None:intergenic 45.0%
TTGTAGTGAATACCTCTGGC+TGG + chr4.3:16642557-16642576 MS.gene09046:CDS 45.0%
TTTAATGAGTCCTGACGAGC+TGG - chr4.3:16642858-16642877 None:intergenic 45.0%
TTTACCTTTAACCCAGCCAG+AGG - chr4.3:16642572-16642591 None:intergenic 45.0%
TTTGACCCGAACTTTCCAAC+AGG - chr4.3:16642795-16642814 None:intergenic 45.0%
! ACAAATGTGATGGGAGGAGT+TGG - chr4.3:16641126-16641145 None:intergenic 45.0%
! AGGAGTTGGACTGGATGTAA+TGG - chr4.3:16641112-16641131 None:intergenic 45.0%
! AGGCACCAGGGTAACTTTTT+AGG - chr4.3:16643336-16643355 None:intergenic 45.0%
! CCACAAACAAATGTGATGGG+AGG - chr4.3:16641132-16641151 None:intergenic 45.0%
! CTGATTACACCTGTGCCATT+TGG + chr4.3:16644242-16644261 MS.gene09046:CDS 45.0%
! GGGAACTGAGACTGTCTTTT+TGG + chr4.3:16642181-16642200 MS.gene09046:intron 45.0%
!! AGGGGCATTGACACAGTTAA+AGG + chr4.3:16644209-16644228 MS.gene09046:CDS 45.0%
!! CCAGAGGTATTCACTACAAG+AGG - chr4.3:16642556-16642575 None:intergenic 45.0%
!! CTTGGTCTTGGTAGGTAACT+TGG + chr4.3:16643354-16643373 MS.gene09046:intron 45.0%
!!! TTTTTAAATTTAAAAGTTTA+AGG + chr4.3:16641490-16641509 MS.gene09046:intron 5.0%
ACACGAAGGCCGCAAAATAG+GGG - chr4.3:16643784-16643803 None:intergenic 50.0%
CAACCTGAGTTTACTCCTCC+TGG + chr4.3:16641249-16641268 MS.gene09046:CDS 50.0%
CAGGAGGAGTAAACTCAGGT+TGG - chr4.3:16641251-16641270 None:intergenic 50.0%
CATGCCTGAAAACACCAGAG+AGG + chr4.3:16641750-16641769 MS.gene09046:CDS 50.0%
CTACACGAAGGCCGCAAAAT+AGG - chr4.3:16643786-16643805 None:intergenic 50.0%
GAGGACTCGTTAGTGGCTTA+GGG - chr4.3:16643557-16643576 None:intergenic 50.0%
GATTCGACGTGTTCTTCACG+AGG + chr4.3:16643154-16643173 MS.gene09046:CDS 50.0%
GCACCCTGGGGAAACAAATA+GGG - chr4.3:16642980-16642999 None:intergenic 50.0%
GCAGCTTGTGACCATTGTTG+TGG - chr4.3:16641084-16641103 None:intergenic 50.0%
TTGGCCCTTTGGCAGTAATG+TGG + chr4.3:16643742-16643761 MS.gene09046:intron 50.0%
! AGACGAGCATCCTTTTGAGC+AGG - chr4.3:16643002-16643021 None:intergenic 50.0%
! GTTGAAGGCCCCCTATTTTG+CGG + chr4.3:16643772-16643791 MS.gene09046:intron 50.0%
! TTTTGCGGCCTTCGTGTAGA+AGG + chr4.3:16643787-16643806 MS.gene09046:intron 50.0%
!! CTCTCTGGTGTTTTCAGGCA+TGG - chr4.3:16641752-16641771 None:intergenic 50.0%
!! TTCTGGAACATGGTCCTGCT+TGG + chr4.3:16643723-16643742 MS.gene09046:intron 50.0%
CACGAAGGCCGCAAAATAGG+GGG - chr4.3:16643783-16643802 None:intergenic 55.0%
GGAGGACTCGTTAGTGGCTT+AGG - chr4.3:16643558-16643577 None:intergenic 55.0%
GGCACCCTGGGGAAACAAAT+AGG - chr4.3:16642981-16643000 None:intergenic 55.0%
TTACCCTGGTGCCTTGGTCT+TGG + chr4.3:16643342-16643361 MS.gene09046:CDS 55.0%
TTACTGCCAAAGGGCCAAGC+AGG - chr4.3:16643740-16643759 None:intergenic 55.0%
! ATCCTTTTGAGCAGGCACCC+TGG - chr4.3:16642994-16643013 None:intergenic 55.0%
! CTACCAAGACCAAGGCACCA+GGG - chr4.3:16643348-16643367 None:intergenic 55.0%
! TCCTTTTGAGCAGGCACCCT+GGG - chr4.3:16642993-16643012 None:intergenic 55.0%
! TGTGATGGGAGGAGTTGGAC+TGG - chr4.3:16641121-16641140 None:intergenic 55.0%
CATGGTCCTGCTTGGCCCTT+TGG + chr4.3:16643731-16643750 MS.gene09046:intron 60.0%
! CCTACCAAGACCAAGGCACC+AGG - chr4.3:16643349-16643368 None:intergenic 60.0%
! CCTTTTGAGCAGGCACCCTG+GGG - chr4.3:16642992-16643011 None:intergenic 60.0%
!! CCTGGTGCCTTGGTCTTGGT+AGG + chr4.3:16643346-16643365 MS.gene09046:intron 60.0%
! CCCCAGGGTGCCTGCTCAAA+AGG + chr4.3:16642989-16643008 MS.gene09046:intron 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 16641042 16644328 16641042 ID=MS.gene09046
chr4.3 mRNA 16641042 16644328 16641042 ID=MS.gene09046.t1;Parent=MS.gene09046
chr4.3 exon 16641042 16641309 16641042 ID=MS.gene09046.t1.exon1;Parent=MS.gene09046.t1
chr4.3 CDS 16641042 16641309 16641042 ID=cds.MS.gene09046.t1;Parent=MS.gene09046.t1
chr4.3 exon 16641738 16641771 16641738 ID=MS.gene09046.t1.exon2;Parent=MS.gene09046.t1
chr4.3 CDS 16641738 16641771 16641738 ID=cds.MS.gene09046.t1;Parent=MS.gene09046.t1
chr4.3 exon 16642417 16642586 16642417 ID=MS.gene09046.t1.exon3;Parent=MS.gene09046.t1
chr4.3 CDS 16642417 16642586 16642417 ID=cds.MS.gene09046.t1;Parent=MS.gene09046.t1
chr4.3 exon 16642700 16642883 16642700 ID=MS.gene09046.t1.exon4;Parent=MS.gene09046.t1
chr4.3 CDS 16642700 16642883 16642700 ID=cds.MS.gene09046.t1;Parent=MS.gene09046.t1
chr4.3 exon 16642995 16643175 16642995 ID=MS.gene09046.t1.exon5;Parent=MS.gene09046.t1
chr4.3 CDS 16642995 16643175 16642995 ID=cds.MS.gene09046.t1;Parent=MS.gene09046.t1
chr4.3 exon 16643264 16643363 16643264 ID=MS.gene09046.t1.exon6;Parent=MS.gene09046.t1
chr4.3 CDS 16643264 16643363 16643264 ID=cds.MS.gene09046.t1;Parent=MS.gene09046.t1
chr4.3 exon 16644201 16644328 16644201 ID=MS.gene09046.t1.exon7;Parent=MS.gene09046.t1
chr4.3 CDS 16644201 16644328 16644201 ID=cds.MS.gene09046.t1;Parent=MS.gene09046.t1
Gene Sequence

>MS.gene09046

ATGGGTTCATCTGGTTTTGTCCTTCCAGACATTTACATCCCACAACAATGGTCACAAGCTGCTAACTCCATTACATCCAGTCCAACTCCTCCCATCACATTTGTTTGTGGAGCAAGTAACTGCGGCAAATCAACCTTCTCGCGAAATCTCCTCAATGTCCTATTAACTAGATGCGACCGAGTTGCTTATCTTGAAACTGATGTTGGCCAACCTGAGTTTACTCCTCCTGGTTTTATCTCTCTTACCATTGTTAATAAAGTTACTCCAGGTAGGTTTTCTAACATATACCTATTCTAACATTTACATGTAATAGAATCACAAGTTAAATAAGTTTTTCGTCGTACATAATTATCAGCTTTCGTTTTAATGTTCTCGTAAAAATTGTTGCATGTTTGTGTCTAATAAAATTCCCACATTTAGTTTTACTACGTGCTAAATTCAAATTTGTTTTTTAAATTTAAAAGTTTAAGGAAAATTAAACAAATTTGAATTTAACATGAATTGAAACCACGTGCTTGAAAATTTTAAACAGACGCAAACTTGTCACTTTTTTCATGGGAGGAAGAAACGAGATTTGATAATTATGTTGGGACAACAATCATATTTAACCCTATATTCATTGATTCAATAATTTTCTATAAAAATGTTCATTATGGATATTGACACGAGTTTTTTTTTGTGTTTTTACTATGTTAGATTTGACGATTCCATGCCTGAAAACACCAGAGAGGTATAAACTATGAAATGTTATCTCATATCTTGATTAACTTTGTACTAATGATTAGTTAACTTTATACTAATGATGTAAATCAAATATTTTGAAACCACGAGAGATGTATAAACTATGAAATTTGATGTTGCCTCAACCTTAACTATTGTATCTTGTTAAATAATCAATTCTTGACTTGACCAATGGTCTCAGACTCAATTACTAGTTTAGATATTGTCAGATTCTTAATAGCATAGATTTGAGGCTTTTGCCACTCACAAAGATAAAAGAATTGTGAATAGTTTACATGCCTTAAGATACTTTCGATCAGAAATTTTCGCAACAGTGCTAGTTTCAGTATCAATACCAGTTTGTATCAATGTCATTAACATGTGATACTGGCACTCATGATATCTCATCTCAATAATTTGGGAACTGAGACTGTCTTTTTGGTTTTTTATTTGTTGCTTTGAATTTGAGGTCCTATTTTGTCACATTGTTATGCAGTCTTCTACAAACAGTGAGTTGGATGAAATTTTGTCTGTTTGTAGTTTGTAGGATATATTTGATTCTTTTAGTGTCGGCCAATCCATGTCTTATTTGAATGAATGCGCTCACTTGTAATGGAGTTGCTTGACTCACTTTCTGTCGTTTATTGCGTAACAGATCCCTTTTTTTTGGTGATGTTTCTTCTCAGAAAGATCCGTCAACATACTTAAAATACATATGTTCTATTTATGATTATTATCATAAGGAGTATTGCACCTTTGATAAGAGACAAAATTCTTCTAAGATTCACTTGCCTCTTGTAGTGAATACCTCTGGCTGGGTTAAAGGTAAACTCGTATATTTTGTATTTTTACTCCAAATAAACTACTGCTTTTCTCGACCAATAACAATATATCAATTATTATGTTACTCACTCAATGTGTTTTGTCTTGCATTTCAGGTGATGGTTATAAGGTATTAGTGAACATGCTAAAATATATTGGTCCCACTCATATAGTTAAGATAGGCATATCCACTGATCTGAAGAATTTACCTGTTGGAAAGTTCGGGTCAAATGGGAAGAATAACAGAAGAGTTAAATTGATTGAGATATATCCAGCTCGTCAGGACTCATTAAATAAATCGTAAGCACAAATAATATAAGTTACCATTTTCAGCTGATAGTTTAGTTCATTATATTTTAGACTTAATTTAAGTATTATAGAATAACAGAATATCCCTATTTGTTTCCCCAGGGTGCCTGCTCAAAAGGATGCTCGTCTTGTGCGCGATTCACGAATAATAGCTTATTTCAGACAGTGCTTTCCTAGTGATTCAGATATTTCAACAATCAAGGATCTTGCAAATTCCTTGACCTCTCACTGTCCTTATAATGTTCCCATCGCAAGCATAAAGATTCGACGTGTTCTTCACGAGGTTAGTTCTAATGCTACTTATCATCTCTTCTGGTTCTGTCTCATTCATTGATCAAAACATTTTACATGACTTATGAATATTTTTGTAGGTCCCAATCTCTGAAATACTCTCCAGCCTGAATGCTAGTATTGTTGGCTTAGCAGTTGAATCTGAAGGACCTAAAAAGTTACCCTGGTGCCTTGGTCTTGGTAGGTAACTTGGTTGACTTCCAAATTCCAAAATGTCTCGTCATAGGTTGATTTCTCAAAATTTAAAAGAATATGAATTATTTATTCATCCTAAACAAAACACCAATGAGTGTTCTATGTACATGAGACCCTAAGGCAGAACAAGAAACTAATTAATGAATAATGACTAAATCCGATGATGTTTGAGATTCCCTAAGCCACTAACGAGTCCTCCAAATGATTCAACTCTATGAATAAAGTTACAAAATAAAGGATATTAGTTACATACACAGGACTATATCTGTTATAGAAACAAACATGCTGACGATCCCCCTCAAGCTGATTAATAGGTATTTACTTTTCCCAGCTTGTATACAATATTCTGGAACATGGTCCTGCTTGGCCCTTTGGCAGTAATGTGGTCAACTAGTTGAAGGCCCCCTATTTTGCGGCCTTCGTGTAGAAGGCTTCAAATGCCTGTAAACAAAAAACAACATTTGGATTATAATGAAAAGATAAATTTAAATTTTGAACTTGATATCATTCAAACTTATGGTAGCAGTGAGGTACACTAAAAGGAATTTGCATTTGTAGGTTGACTTCATTATATCTCATATTTCTGTTGGCTGTAACAGGAGTATCATGAGTTGCAACACATCTTAATGGATAATTACCTTTTTAATGTATCAAACATCCACAAATAGAAAAGATAAAGAATAAATTCATATTCCTCTGGTCTTCTGATCTTGTAATGACGAGCACATGGATCATACAGCCATCTAATTTTCTGGCTGTCATGCATTTGAGTAATTCTTAGCATCATTACTGTTAAAGGCGTTTTGATTTCTTGTGATTCAGGTATTGTGAGGGGCATTGACACAGTTAAAGGCATGCTCTATCTGATTACACCTGTGCCATTTGGTTCTCTGAAAAAAGTCAATCTCTTGCTTCAGGGTAATATCCAAATTCCTACAAGTTTATTGCAG
Protein sequence

>MS.gene09046.t1

MGSSGFVLPDIYIPQQWSQAANSITSSPTPPITFVCGASNCGKSTFSRNLLNVLLTRCDRVAYLETDVGQPEFTPPGFISLTIVNKVTPDLTIPCLKTPERSLFFGDVSSQKDPSTYLKYICSIYDYYHKEYCTFDKRQNSSKIHLPLVVNTSGWVKGDGYKVLVNMLKYIGPTHIVKIGISTDLKNLPVGKFGSNGKNNRRVKLIEIYPARQDSLNKSVPAQKDARLVRDSRIIAYFRQCFPSDSDISTIKDLANSLTSHCPYNVPIASIKIRRVLHEVPISEILSSLNASIVGLAVESEGPKKLPWCLGLGIVRGIDTVKGMLYLITPVPFGSLKKVNLLLQGNIQIPTSLLQ