AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene09188

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09188.t1 MTR_8g080150 95.238 357 12 3 1 355 1 354 0.0 686
MS.gene09188.t1 MTR_8g080140 68.661 351 101 5 9 354 7 353 1.57e-168 474
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09188.t1 AT5G11010 56.034 348 144 5 12 355 10 352 4.63e-135 389
MS.gene09188.t1 AT5G11010 56.034 348 144 5 12 355 10 352 4.63e-135 389
MS.gene09188.t1 AT5G11010 56.034 348 144 5 12 355 10 352 4.63e-135 389
MS.gene09188.t1 AT5G11010 54.426 305 130 5 12 312 10 309 1.09e-113 333

Find 80 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAATAAACTTGTAGGAATT+TGG 0.140890 4.2:-15000704 None:intergenic
AATACCTCTGGCTGGGTTAA+AGG 0.251617 4.2:+14998968 MS.gene09188:CDS
CTTACCTGCAATAAACTTGT+AGG 0.273363 4.2:-15000711 None:intergenic
CCATTTGACCCGAACTTTCC+AGG 0.282127 4.2:-14999195 None:intergenic
CATTGCAGGTGATGGTTATA+AGG 0.288067 4.2:+14999095 MS.gene09188:intron
AAAAGTCAACCTCTTGCTTC+AGG 0.296771 4.2:+15000674 MS.gene09188:CDS
AGCCTGAATGCTAGTATTGT+TGG 0.330336 4.2:+14999688 MS.gene09188:CDS
AAGAATTTACCTGGAAAGTT+CGG 0.336338 4.2:+14999186 MS.gene09188:CDS
CTTGCGATGGGAACATTATA+AGG 0.336390 4.2:-14999525 None:intergenic
CTGATTACACCTGTGCCATT+TGG 0.342406 4.2:+15000642 MS.gene09188:CDS
CTCGGTCGCATCTAGTTAAT+AGG 0.357472 4.2:-14997604 None:intergenic
CCTGGAAAGTTCGGGTCAAA+TGG 0.360089 4.2:+14999195 MS.gene09188:CDS
CTGGAGAGTATTTCAGAGAT+TGG 0.360131 4.2:-14999667 None:intergenic
CCTCCCATCACATTTGTTTG+TGG 0.372621 4.2:+14997533 MS.gene09188:CDS
AATTCCTACAAGTTTATTGC+AGG 0.374142 4.2:+15000707 MS.gene09188:CDS
CAACCTGAGTTTACTCCTCC+TGG 0.374377 4.2:+14997653 MS.gene09188:CDS
CCCCAGGGTGCCTGCTCAAA+AGG 0.383947 4.2:+14999389 MS.gene09188:intron
AGGGGCATTGACACAGTTAA+AGG 0.386318 4.2:+15000609 MS.gene09188:CDS
GAAAGAGCAATGGGTTCATC+TGG 0.388190 4.2:+14997437 None:intergenic
TTACCCTGGTGCCTTGGTCT+TGG 0.389061 4.2:+14999742 MS.gene09188:CDS
AGCCAACAATACTAGCATTC+AGG 0.390103 4.2:-14999690 None:intergenic
TTGTAGTGAATACCTCTGGC+TGG 0.403286 4.2:+14998960 MS.gene09188:CDS
GTGAACATGCTAAAATATAT+TGG 0.405008 4.2:+14999123 MS.gene09188:CDS
AGAATTTACCTGGAAAGTTC+GGG 0.410696 4.2:+14999187 MS.gene09188:CDS
TATTTATGATTATTATCATA+AGG 0.411756 4.2:+14998886 MS.gene09188:CDS
TTTCATAGTTTATACCTCTC+TGG 0.415607 4.2:-14998167 None:intergenic
TTCAGATATTTCAACAATCA+AGG 0.444136 4.2:+14999473 MS.gene09188:CDS
TGGAGAGTATTTCAGAGATT+GGG 0.453316 4.2:-14999666 None:intergenic
CCCACTCATATAGTTAAGAT+AGG 0.455843 4.2:+14999147 MS.gene09188:CDS
TGAGATATATCCAGCTCGTC+AGG 0.461392 4.2:+14999245 MS.gene09188:CDS
CTGGAAAGTTCGGGTCAAAT+GGG 0.477512 4.2:+14999196 MS.gene09188:CDS
TTTAATGAGTCCTGACGAGC+TGG 0.479415 4.2:-14999255 None:intergenic
CAGCTTGTGACCATTGTTGT+GGG 0.481609 4.2:-14997484 None:intergenic
AGGAGTTGGACTGGATGTAA+TGG 0.489408 4.2:-14997513 None:intergenic
AAAGTCAACCTCTTGCTTCA+GGG 0.498916 4.2:+15000675 MS.gene09188:CDS
ACAAATGTGATGGGAGGAGT+TGG 0.500253 4.2:-14997527 None:intergenic
GCAGCTTGTGACCATTGTTG+TGG 0.508024 4.2:-14997485 None:intergenic
TGTTGTGGGATGTAAATGTC+TGG 0.513328 4.2:-14997470 None:intergenic
CCTCTTGTAGTGAATACCTC+TGG 0.514438 4.2:+14998956 MS.gene09188:CDS
TTCAGAGAACCAAATGGCAC+AGG 0.515584 4.2:-15000651 None:intergenic
TTGTGATTCAGGTATTGTGA+GGG 0.527148 4.2:+15000590 MS.gene09188:intron
CCTACCAAGACCAAGGCACC+AGG 0.529524 4.2:-14999746 None:intergenic
TCGAATCTTTATGCTTGCGA+TGG 0.529810 4.2:-14999538 None:intergenic
GCTTATCTTGAAACTGATGT+TGG 0.537454 4.2:+14997629 MS.gene09188:CDS
TGTAGTGAATACCTCTGGCT+GGG 0.538425 4.2:+14998961 MS.gene09188:CDS
TGCTCCACAAACAAATGTGA+TGG 0.541521 4.2:-14997537 None:intergenic
ATTGTTAATAAAGTTACTCC+AGG 0.544370 4.2:+14997692 MS.gene09188:CDS
AACAATACTAGCATTCAGGC+TGG 0.547070 4.2:-14999686 None:intergenic
AGGTAAATTCTTCAGATCAG+TGG 0.550384 4.2:-14999175 None:intergenic
CTTGTGATTCAGGTATTGTG+AGG 0.553596 4.2:+15000589 MS.gene09188:intron
CGAATCTTTATGCTTGCGAT+GGG 0.557731 4.2:-14999537 None:intergenic
GGCTTAGCAGTTGAATCTGA+AGG 0.565104 4.2:+14999709 MS.gene09188:CDS
ATAAGGACAGTGAGAGGTCA+AGG 0.568437 4.2:-14999508 None:intergenic
ATTGAGGAGATTTCGCGAGA+AGG 0.573264 4.2:-14997579 None:intergenic
TGTGATTCAGGTATTGTGAG+GGG 0.578885 4.2:+15000591 MS.gene09188:intron
TGGAGTAACTTTATTAACAA+TGG 0.579021 4.2:-14997690 None:intergenic
GCCTATCTTAACTATATGAG+TGG 0.583863 4.2:-14999148 None:intergenic
ACTGATCTGAAGAATTTACC+TGG 0.591590 4.2:+14999177 MS.gene09188:CDS
TGTGATGGGAGGAGTTGGAC+TGG 0.596371 4.2:-14997522 None:intergenic
CCAGAGGTATTCACTACAAG+AGG 0.598694 4.2:-14998956 None:intergenic
GGATATTACCCTGAAGCAAG+AGG 0.598881 4.2:-15000683 None:intergenic
CAGGAGGAGTAAACTCAGGT+TGG 0.603274 4.2:-14997652 None:intergenic
ATGGTAAGAGAGATAAAACC+AGG 0.613877 4.2:-14997671 None:intergenic
GCTCCACAAACAAATGTGAT+GGG 0.614241 4.2:-14997536 None:intergenic
ATCTAGTTAATAGGACATTG+AGG 0.620697 4.2:-14997595 None:intergenic
CAGTTTCAAGATAAGCAACT+CGG 0.625840 4.2:-14997622 None:intergenic
CCTATCTTAACTATATGAGT+GGG 0.628881 4.2:-14999147 None:intergenic
GTTGAAATATCTGAATCACT+AGG 0.636570 4.2:-14999465 None:intergenic
TTTACCTTTAACCCAGCCAG+AGG 0.641811 4.2:-14998972 None:intergenic
AAACCAGGAGGAGTAAACTC+AGG 0.646510 4.2:-14997656 None:intergenic
AACATTATAAGGACAGTGAG+AGG 0.648592 4.2:-14999514 None:intergenic
GATTCGACGTGTTCTTCACG+AGG 0.658346 4.2:+14999554 MS.gene09188:CDS
GTGGGATGTAAATGTCTGGA+AGG 0.660248 4.2:-14997466 None:intergenic
ACATTTACATCCCACAACAA+TGG 0.661470 4.2:+14997474 MS.gene09188:CDS
CCACAAACAAATGTGATGGG+AGG 0.666150 4.2:-14997533 None:intergenic
GTTTGTGGAGCAAGTAACTG+CGG 0.670641 4.2:+14997548 MS.gene09188:CDS
CAAGTTACCTACCAAGACCA+AGG 0.684244 4.2:-14999753 None:intergenic
CTACCAAGACCAAGGCACCA+GGG 0.712305 4.2:-14999745 None:intergenic
GTAAGAGAGATAAAACCAGG+AGG 0.735353 4.2:-14997668 None:intergenic
CATGCCTGAAAACACCAGAG+AGG 0.772404 4.2:+14998153 MS.gene09188:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATTGATATATTGTTAT+TGG - chr4.2:14999046-14999065 None:intergenic 10.0%
!! TATTTATGATTATTATCATA+AGG + chr4.2:14998886-14998905 MS.gene09188:CDS 10.0%
!! ATTATTGAATCAATGAATAT+AGG - chr4.2:14998058-14998077 None:intergenic 15.0%
!! TGAATAAAGTTACAAAATAA+AGG + chr4.2:14999994-15000013 MS.gene09188:intron 15.0%
!! TTATTGAATCAATGAATATA+GGG - chr4.2:14998057-14998076 None:intergenic 15.0%
!! TTCTATAAAAATGTTCATTA+TGG + chr4.2:14998079-14998098 MS.gene09188:intron 15.0%
!! ATATCTCATCTCAATAATTT+GGG + chr4.2:14998564-14998583 MS.gene09188:intron 20.0%
!! TGTAAACAAAAAACAACATT+TGG + chr4.2:15000221-15000240 MS.gene09188:intron 20.0%
!! TTAACAAGATACAATAGTTA+AGG - chr4.2:14998314-14998333 None:intergenic 20.0%
!! TTACATGTAAATGTTAGAAT+AGG - chr4.2:14997736-14997755 None:intergenic 20.0%
!!! AAATGTTGTTTTTTGTTTAC+AGG - chr4.2:15000222-15000241 None:intergenic 20.0%
!!! ATATTTGATTCTTTTAGTGT+CGG + chr4.2:14998715-14998734 MS.gene09188:intron 20.0%
!!! ATGTATTTTAAGTATGTTGA+CGG - chr4.2:14998860-14998879 None:intergenic 20.0%
!!! ATTATATCTCATATTTCTGT+TGG + chr4.2:15000346-15000365 MS.gene09188:intron 20.0%
!!! TGACTTATGAATATTTTTGT+AGG + chr4.2:14999642-14999661 MS.gene09188:intron 20.0%
!!! TTCTTTATCTTTTCTATTTG+TGG - chr4.2:15000440-15000459 None:intergenic 20.0%
! AAGAAACATCACCAAAAAAA+AGG - chr4.2:14998826-14998845 None:intergenic 25.0%
! AGAAACATCACCAAAAAAAA+GGG - chr4.2:14998825-14998844 None:intergenic 25.0%
! AGAATAAATTCATATTCCTC+TGG + chr4.2:15000456-15000475 MS.gene09188:intron 25.0%
! ATTCATAGAGTTGAATCATT+TGG - chr4.2:14999979-14999998 None:intergenic 25.0%
! ATTGTTAATAAAGTTACTCC+AGG + chr4.2:14997692-14997711 MS.gene09188:CDS 25.0%
! CTTGATATCATTCAAACTTA+TGG + chr4.2:15000276-15000295 MS.gene09188:intron 25.0%
! GATATCTCATCTCAATAATT+TGG + chr4.2:14998563-14998582 MS.gene09188:intron 25.0%
! GGATGTTTGATACATTAAAA+AGG - chr4.2:15000419-15000438 None:intergenic 25.0%
! GTGAACATGCTAAAATATAT+TGG + chr4.2:14999123-14999142 MS.gene09188:CDS 25.0%
! TAAAAGGAATTTGCATTTGT+AGG + chr4.2:15000315-15000334 MS.gene09188:intron 25.0%
! TGGAGTAACTTTATTAACAA+TGG - chr4.2:14997693-14997712 None:intergenic 25.0%
! TTAATGACATTGATACAAAC+TGG - chr4.2:14998523-14998542 None:intergenic 25.0%
! TTCAGATATTTCAACAATCA+AGG + chr4.2:14999473-14999492 MS.gene09188:CDS 25.0%
! TTCTTAATAGCATAGATTTG+AGG + chr4.2:14998396-14998415 MS.gene09188:intron 25.0%
!! AATTCTTTTATCTATGTGAG+TGG - chr4.2:14998428-14998447 None:intergenic 25.0%
!! ACGAGATTTGATAATTATGT+TGG + chr4.2:14998013-14998032 MS.gene09188:intron 25.0%
!! AGAATTTTGTCTCTTATCAA+AGG - chr4.2:14998921-14998940 None:intergenic 25.0%
!! CGAGATTTGATAATTATGTT+GGG + chr4.2:14998014-14998033 MS.gene09188:intron 25.0%
!!! AAACTTGTCACTTTTTTCAT+GGG + chr4.2:14997982-14998001 MS.gene09188:intron 25.0%
!!! TATTTGTTGCTTTGAATTTG+AGG + chr4.2:14998612-14998631 MS.gene09188:intron 25.0%
AAGAATTTACCTGGAAAGTT+CGG + chr4.2:14999186-14999205 MS.gene09188:CDS 30.0%
AAGCTGATAATTATGTACGA+CGG - chr4.2:14997784-14997803 None:intergenic 30.0%
AATACCTATTAATCAGCTTG+AGG - chr4.2:15000078-15000097 None:intergenic 30.0%
AATTCCTACAAGTTTATTGC+AGG + chr4.2:15000707-15000726 MS.gene09188:CDS 30.0%
AGATACAATAGTTAAGGTTG+AGG - chr4.2:14998308-14998327 None:intergenic 30.0%
AGGATATTAGTTACATACAC+AGG + chr4.2:15000014-15000033 MS.gene09188:intron 30.0%
ATACCTATTAATCAGCTTGA+GGG - chr4.2:15000077-15000096 None:intergenic 30.0%
ATCTAGTTAATAGGACATTG+AGG - chr4.2:14997598-14997617 None:intergenic 30.0%
ATGTCATTAACATGTGATAC+TGG + chr4.2:14998533-14998552 MS.gene09188:intron 30.0%
CACGTAGTAAAACTAAATGT+GGG - chr4.2:14997858-14997877 None:intergenic 30.0%
CAGAATATTGTATACAAGCT+GGG - chr4.2:15000108-15000127 None:intergenic 30.0%
CATTCAAATAAGACATGGAT+TGG - chr4.2:14998741-14998760 None:intergenic 30.0%
CCTATCTTAACTATATGAGT+GGG - chr4.2:14999150-14999169 None:intergenic 30.0%
CTTAGCATCATTACTGTTAA+AGG + chr4.2:15000555-15000574 MS.gene09188:intron 30.0%
GAGAAAAGCAGTAGTTTATT+TGG - chr4.2:14999021-14999040 None:intergenic 30.0%
GCAATAAACTTGTAGGAATT+TGG - chr4.2:15000707-15000726 None:intergenic 30.0%
GCATAACAATGTGACAAAAT+AGG - chr4.2:14998639-14998658 None:intergenic 30.0%
GCATTCATTCAAATAAGACA+TGG - chr4.2:14998746-14998765 None:intergenic 30.0%
GTTGAAATATCTGAATCACT+AGG - chr4.2:14999468-14999487 None:intergenic 30.0%
TATATGTTAGAAAACCTACC+TGG - chr4.2:14997713-14997732 None:intergenic 30.0%
TTAATAAAGTTACTCCAGGT+AGG + chr4.2:14997696-14997715 MS.gene09188:intron 30.0%
TTATTACTTTGAGCTACCAA+AGG + chr4.2:15000807-15000826 MS.gene09188:intron 30.0%
TTTCATAGTTTATACCTCTC+TGG - chr4.2:14998170-14998189 None:intergenic 30.0%
! TTTGTCTGTTTGTAGTTTGT+AGG + chr4.2:14998690-14998709 MS.gene09188:intron 30.0%
!! GTATACAATATTCTGGAACA+TGG + chr4.2:15000113-15000132 MS.gene09188:intron 30.0%
!! GTTTAAAATTTTCAAGCACG+TGG - chr4.2:14997956-14997975 None:intergenic 30.0%
!! TTCTGATCGAAAGTATCTTA+AGG - chr4.2:14998467-14998486 None:intergenic 30.0%
!!! ACTTTTTTCAGAGAACCAAA+TGG - chr4.2:15000660-15000679 None:intergenic 30.0%
!!! CAAACTTGTCACTTTTTTCA+TGG + chr4.2:14997981-14998000 MS.gene09188:intron 30.0%
!!! GTTTTGATTTCTTGTGATTC+AGG + chr4.2:15000579-15000598 MS.gene09188:intron 30.0%
!!! TGTTTTTTGTTTACAGGCAT+TGG - chr4.2:15000216-15000235 None:intergenic 30.0%
AACATTATAAGGACAGTGAG+AGG - chr4.2:14999517-14999536 None:intergenic 35.0%
ACATTTACATCCCACAACAA+TGG + chr4.2:14997474-14997493 MS.gene09188:CDS 35.0%
ACCAAAGGAAAACTTTCTGT+TGG + chr4.2:15000822-15000841 MS.gene09188:intron 35.0%
ACTAAACTAGCAGCTGAAAA+TGG - chr4.2:14999310-14999329 None:intergenic 35.0%
ACTGATCTGAAGAATTTACC+TGG + chr4.2:14999177-14999196 MS.gene09188:CDS 35.0%
AGAATTTACCTGGAAAGTTC+GGG + chr4.2:14999187-14999206 MS.gene09188:CDS 35.0%
AGGTAAATTCTTCAGATCAG+TGG - chr4.2:14999178-14999197 None:intergenic 35.0%
ATGACAGCCAGAAAATTAGA+TGG - chr4.2:15000521-15000540 None:intergenic 35.0%
ATGGTAAGAGAGATAAAACC+AGG - chr4.2:14997674-14997693 None:intergenic 35.0%
ATTCCAAAATGTCTCGTCAT+AGG + chr4.2:14999788-14999807 MS.gene09188:intron 35.0%
CAGTTTCAAGATAAGCAACT+CGG - chr4.2:14997625-14997644 None:intergenic 35.0%
CATAGAGTTGAATCATTTGG+AGG - chr4.2:14999976-14999995 None:intergenic 35.0%
CATAGTTTATACATCTCTCG+TGG - chr4.2:14998272-14998291 None:intergenic 35.0%
CATGTACATAGAACACTCAT+TGG - chr4.2:14999869-14999888 None:intergenic 35.0%
CCAGAATATTGTATACAAGC+TGG - chr4.2:15000109-15000128 None:intergenic 35.0%
CCAGCTTGTATACAATATTC+TGG + chr4.2:15000106-15000125 MS.gene09188:intron 35.0%
CCCACTCATATAGTTAAGAT+AGG + chr4.2:14999147-14999166 MS.gene09188:CDS 35.0%
CTTACCTGCAATAAACTTGT+AGG - chr4.2:15000714-15000733 None:intergenic 35.0%
GCACGTAGTAAAACTAAATG+TGG - chr4.2:14997859-14997878 None:intergenic 35.0%
GCCTATCTTAACTATATGAG+TGG - chr4.2:14999151-14999170 None:intergenic 35.0%
GCTTATCTTGAAACTGATGT+TGG + chr4.2:14997629-14997648 MS.gene09188:CDS 35.0%
TAATGTGGTCAACTAGTTGA+AGG + chr4.2:15000157-15000176 MS.gene09188:intron 35.0%
TACCTATTAATCAGCTTGAG+GGG - chr4.2:15000076-15000095 None:intergenic 35.0%
TAGGGAATCTCAAACATCAT+CGG - chr4.2:14999939-14999958 None:intergenic 35.0%
TAGTTTCTTGTTCTGCCTTA+GGG - chr4.2:14999895-14999914 None:intergenic 35.0%
TGAGTTGCAACACATCTTAA+TGG + chr4.2:15000386-15000405 MS.gene09188:intron 35.0%
TTAGTTTCTTGTTCTGCCTT+AGG - chr4.2:14999896-14999915 None:intergenic 35.0%
! ACCAACAGAAAGTTTTCCTT+TGG - chr4.2:15000826-15000845 None:intergenic 35.0%
! ATATTTCTGTTGGCTGTAAC+AGG + chr4.2:15000356-15000375 MS.gene09188:intron 35.0%
! ATGCTACTTATCATCTCTTC+TGG + chr4.2:14999586-14999605 MS.gene09188:intron 35.0%
! CATACAGCCATCTAATTTTC+TGG + chr4.2:15000511-15000530 MS.gene09188:intron 35.0%
! TCAATTCTTGACTTGACCAA+TGG + chr4.2:14998338-14998357 MS.gene09188:intron 35.0%
! TTGTGATTCAGGTATTGTGA+GGG + chr4.2:15000590-15000609 MS.gene09188:intron 35.0%
!! TGGAGAGTATTTCAGAGATT+GGG - chr4.2:14999669-14999688 None:intergenic 35.0%
!!! CACTCATTGGTGTTTTGTTT+AGG - chr4.2:14999856-14999875 None:intergenic 35.0%
!!! CGTAACAGATCCCTTTTTTT+TGG + chr4.2:14998812-14998831 MS.gene09188:intron 35.0%
!!! CTTTCTGTTGGTTTTTCCTT+AGG + chr4.2:15000834-15000853 MS.gene09188:intron 35.0%
!!! TGACGAGACATTTTGGAATT+TGG - chr4.2:14999787-14999806 None:intergenic 35.0%
AAAAGTCAACCTCTTGCTTC+AGG + chr4.2:15000674-15000693 MS.gene09188:CDS 40.0%
AAAGTCAACCTCTTGCTTCA+GGG + chr4.2:15000675-15000694 MS.gene09188:CDS 40.0%
AACAATACTAGCATTCAGGC+TGG - chr4.2:14999689-14999708 None:intergenic 40.0%
AAGGACCTAAAAAGTTACCC+TGG + chr4.2:14999728-14999747 MS.gene09188:CDS 40.0%
ACCTATTAATCAGCTTGAGG+GGG - chr4.2:15000075-15000094 None:intergenic 40.0%
AGCCAACAATACTAGCATTC+AGG - chr4.2:14999693-14999712 None:intergenic 40.0%
AGCCTGAATGCTAGTATTGT+TGG + chr4.2:14999688-14999707 MS.gene09188:CDS 40.0%
ATATCCCTATTTGTTTCCCC+AGG + chr4.2:14999373-14999392 MS.gene09188:intron 40.0%
ATCTTGTAATGACGAGCACA+TGG + chr4.2:15000486-15000505 MS.gene09188:intron 40.0%
ATGAATGCGCTCACTTGTAA+TGG + chr4.2:14998758-14998777 MS.gene09188:intron 40.0%
CGAATCTTTATGCTTGCGAT+GGG - chr4.2:14999540-14999559 None:intergenic 40.0%
CTTGCGATGGGAACATTATA+AGG - chr4.2:14999528-14999547 None:intergenic 40.0%
GCTCCACAAACAAATGTGAT+GGG - chr4.2:14997539-14997558 None:intergenic 40.0%
GTAAGAGAGATAAAACCAGG+AGG - chr4.2:14997671-14997690 None:intergenic 40.0%
GTCTTCTACAAACAGTGAGT+TGG + chr4.2:14998660-14998679 MS.gene09188:intron 40.0%
TAGCAGTGAGGTACACTAAA+AGG + chr4.2:15000299-15000318 MS.gene09188:intron 40.0%
TATCCCTATTTGTTTCCCCA+GGG + chr4.2:14999374-14999393 MS.gene09188:intron 40.0%
TCAAACTTATGGTAGCAGTG+AGG + chr4.2:15000287-15000306 MS.gene09188:intron 40.0%
TCGAATCTTTATGCTTGCGA+TGG - chr4.2:14999541-14999560 None:intergenic 40.0%
TCTATGTACATGAGACCCTA+AGG + chr4.2:14999877-14999896 MS.gene09188:intron 40.0%
TGCTCCACAAACAAATGTGA+TGG - chr4.2:14997540-14997559 None:intergenic 40.0%
TGTTGTGGGATGTAAATGTC+TGG - chr4.2:14997473-14997492 None:intergenic 40.0%
TTACAAGATCAGAAGACCAG+AGG - chr4.2:15000475-15000494 None:intergenic 40.0%
TTGACCACATTACTGCCAAA+GGG - chr4.2:15000149-15000168 None:intergenic 40.0%
! CAACCTATGACGAGACATTT+TGG - chr4.2:14999794-14999813 None:intergenic 40.0%
! CATTGCAGGTGATGGTTATA+AGG + chr4.2:14999095-14999114 MS.gene09188:intron 40.0%
! CTTGTGATTCAGGTATTGTG+AGG + chr4.2:15000589-15000608 MS.gene09188:intron 40.0%
! GTAATTGAGTCTGAGACCAT+TGG - chr4.2:14998357-14998376 None:intergenic 40.0%
! TATACCTCTCTGGTGTTTTC+AGG - chr4.2:14998160-14998179 None:intergenic 40.0%
! TGTGATTCAGGTATTGTGAG+GGG + chr4.2:15000591-15000610 MS.gene09188:intron 40.0%
! TGTGTTTTGTCTTGCATTGC+AGG + chr4.2:14999081-14999100 MS.gene09188:intron 40.0%
!! CTGGAGAGTATTTCAGAGAT+TGG - chr4.2:14999670-14999689 None:intergenic 40.0%
!!! CTTGTCACTTTTTTCATGGG+AGG + chr4.2:14997985-14998004 MS.gene09188:intron 40.0%
AAAAAGTTACCCTGGTGCCT+TGG + chr4.2:14999736-14999755 MS.gene09188:CDS 45.0%
AAACCAGGAGGAGTAAACTC+AGG - chr4.2:14997659-14997678 None:intergenic 45.0%
AATACCTCTGGCTGGGTTAA+AGG + chr4.2:14998968-14998987 MS.gene09188:CDS 45.0%
ATAAGGACAGTGAGAGGTCA+AGG - chr4.2:14999511-14999530 None:intergenic 45.0%
ATTGAGGAGATTTCGCGAGA+AGG - chr4.2:14997582-14997601 None:intergenic 45.0%
ATTGGAAGCCTTCTACACGA+AGG - chr4.2:15000198-15000217 None:intergenic 45.0%
CAAGTTACCTACCAAGACCA+AGG - chr4.2:14999756-14999775 None:intergenic 45.0%
CAGCTTGTGACCATTGTTGT+GGG - chr4.2:14997487-14997506 None:intergenic 45.0%
CCTCCCATCACATTTGTTTG+TGG + chr4.2:14997533-14997552 MS.gene09188:CDS 45.0%
CCTCTTGTAGTGAATACCTC+TGG + chr4.2:14998956-14998975 MS.gene09188:CDS 45.0%
CTCGGTCGCATCTAGTTAAT+AGG - chr4.2:14997607-14997626 None:intergenic 45.0%
CTGGAAAGTTCGGGTCAAAT+GGG + chr4.2:14999196-14999215 MS.gene09188:CDS 45.0%
GGATATTACCCTGAAGCAAG+AGG - chr4.2:15000686-15000705 None:intergenic 45.0%
GGCTTAGCAGTTGAATCTGA+AGG + chr4.2:14999709-14999728 MS.gene09188:CDS 45.0%
GTGGGATGTAAATGTCTGGA+AGG - chr4.2:14997469-14997488 None:intergenic 45.0%
GTTGACCACATTACTGCCAA+AGG - chr4.2:15000150-15000169 None:intergenic 45.0%
GTTTGTGGAGCAAGTAACTG+CGG + chr4.2:14997548-14997567 MS.gene09188:CDS 45.0%
TACACGAAGGCCGCAAAATA+GGG - chr4.2:15000185-15000204 None:intergenic 45.0%
TCATTTGGAGGACTCGTTAG+TGG - chr4.2:14999964-14999983 None:intergenic 45.0%
TCCCCCTCAAGCTGATTAAT+AGG + chr4.2:15000071-15000090 MS.gene09188:intron 45.0%
TGAGATATATCCAGCTCGTC+AGG + chr4.2:14999245-14999264 MS.gene09188:CDS 45.0%
TGTAGTGAATACCTCTGGCT+GGG + chr4.2:14998961-14998980 MS.gene09188:CDS 45.0%
TTCAGAGAACCAAATGGCAC+AGG - chr4.2:15000654-15000673 None:intergenic 45.0%
TTGTAGTGAATACCTCTGGC+TGG + chr4.2:14998960-14998979 MS.gene09188:CDS 45.0%
TTGTCTTGCATTGCAGGTGA+TGG + chr4.2:14999087-14999106 MS.gene09188:intron 45.0%
TTTAATGAGTCCTGACGAGC+TGG - chr4.2:14999258-14999277 None:intergenic 45.0%
TTTACCTTTAACCCAGCCAG+AGG - chr4.2:14998975-14998994 None:intergenic 45.0%
! ACAAATGTGATGGGAGGAGT+TGG - chr4.2:14997530-14997549 None:intergenic 45.0%
! AGGAGTTGGACTGGATGTAA+TGG - chr4.2:14997516-14997535 None:intergenic 45.0%
! AGGCACCAGGGTAACTTTTT+AGG - chr4.2:14999736-14999755 None:intergenic 45.0%
! CCACAAACAAATGTGATGGG+AGG - chr4.2:14997536-14997555 None:intergenic 45.0%
! CTGATTACACCTGTGCCATT+TGG + chr4.2:15000642-15000661 MS.gene09188:CDS 45.0%
! GGGAACTGAGACTGTCTTTT+TGG + chr4.2:14998584-14998603 MS.gene09188:intron 45.0%
!! AGGGGCATTGACACAGTTAA+AGG + chr4.2:15000609-15000628 MS.gene09188:CDS 45.0%
!! CCAGAGGTATTCACTACAAG+AGG - chr4.2:14998959-14998978 None:intergenic 45.0%
!! CTTGGTCTTGGTAGGTAACT+TGG + chr4.2:14999754-14999773 MS.gene09188:intron 45.0%
!!! TTTTTAAATTTAAAAGTTTA+AGG + chr4.2:14997894-14997913 MS.gene09188:intron 5.0%
ACACGAAGGCCGCAAAATAG+GGG - chr4.2:15000184-15000203 None:intergenic 50.0%
CAACCTGAGTTTACTCCTCC+TGG + chr4.2:14997653-14997672 MS.gene09188:CDS 50.0%
CAGGAGGAGTAAACTCAGGT+TGG - chr4.2:14997655-14997674 None:intergenic 50.0%
CATGCCTGAAAACACCAGAG+AGG + chr4.2:14998153-14998172 MS.gene09188:CDS 50.0%
CCATTTGACCCGAACTTTCC+AGG - chr4.2:14999198-14999217 None:intergenic 50.0%
CCTGGAAAGTTCGGGTCAAA+TGG + chr4.2:14999195-14999214 MS.gene09188:CDS 50.0%
CTACACGAAGGCCGCAAAAT+AGG - chr4.2:15000186-15000205 None:intergenic 50.0%
GAGGACTCGTTAGTGGCTTA+GGG - chr4.2:14999957-14999976 None:intergenic 50.0%
GATTCGACGTGTTCTTCACG+AGG + chr4.2:14999554-14999573 MS.gene09188:CDS 50.0%
GCACCCTGGGGAAACAAATA+GGG - chr4.2:14999380-14999399 None:intergenic 50.0%
GCAGCTTGTGACCATTGTTG+TGG - chr4.2:14997488-14997507 None:intergenic 50.0%
TTGGCCCTTTGGCAGTAATG+TGG + chr4.2:15000142-15000161 MS.gene09188:intron 50.0%
! AGACGAGCATCCTTTTGAGC+AGG - chr4.2:14999402-14999421 None:intergenic 50.0%
! GTTGAAGGCCCCCTATTTTG+CGG + chr4.2:15000172-15000191 MS.gene09188:intron 50.0%
! TTTTGCGGCCTTCGTGTAGA+AGG + chr4.2:15000187-15000206 MS.gene09188:intron 50.0%
!! CTCTCTGGTGTTTTCAGGCA+TGG - chr4.2:14998155-14998174 None:intergenic 50.0%
!! TTCTGGAACATGGTCCTGCT+TGG + chr4.2:15000123-15000142 MS.gene09188:intron 50.0%
CACGAAGGCCGCAAAATAGG+GGG - chr4.2:15000183-15000202 None:intergenic 55.0%
GGAGGACTCGTTAGTGGCTT+AGG - chr4.2:14999958-14999977 None:intergenic 55.0%
GGCACCCTGGGGAAACAAAT+AGG - chr4.2:14999381-14999400 None:intergenic 55.0%
TTACCCTGGTGCCTTGGTCT+TGG + chr4.2:14999742-14999761 MS.gene09188:CDS 55.0%
TTACTGCCAAAGGGCCAAGC+AGG - chr4.2:15000140-15000159 None:intergenic 55.0%
! ATCCTTTTGAGCAGGCACCC+TGG - chr4.2:14999394-14999413 None:intergenic 55.0%
! CTACCAAGACCAAGGCACCA+GGG - chr4.2:14999748-14999767 None:intergenic 55.0%
! TCCTTTTGAGCAGGCACCCT+GGG - chr4.2:14999393-14999412 None:intergenic 55.0%
! TGTGATGGGAGGAGTTGGAC+TGG - chr4.2:14997525-14997544 None:intergenic 55.0%
CATGGTCCTGCTTGGCCCTT+TGG + chr4.2:15000131-15000150 MS.gene09188:intron 60.0%
! CCTACCAAGACCAAGGCACC+AGG - chr4.2:14999749-14999768 None:intergenic 60.0%
! CCTTTTGAGCAGGCACCCTG+GGG - chr4.2:14999392-14999411 None:intergenic 60.0%
!! CCTGGTGCCTTGGTCTTGGT+AGG + chr4.2:14999746-14999765 MS.gene09188:intron 60.0%
! CCCCAGGGTGCCTGCTCAAA+AGG + chr4.2:14999389-14999408 MS.gene09188:intron 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 14997446 15000864 14997446 ID=MS.gene09188
chr4.2 mRNA 14997446 15000864 14997446 ID=MS.gene09188.t1;Parent=MS.gene09188
chr4.2 exon 14997446 14997713 14997446 ID=MS.gene09188.t1.exon1;Parent=MS.gene09188.t1
chr4.2 CDS 14997446 14997713 14997446 ID=cds.MS.gene09188.t1;Parent=MS.gene09188.t1
chr4.2 exon 14998141 14998174 14998141 ID=MS.gene09188.t1.exon2;Parent=MS.gene09188.t1
chr4.2 CDS 14998141 14998174 14998141 ID=cds.MS.gene09188.t1;Parent=MS.gene09188.t1
chr4.2 exon 14998820 14998989 14998820 ID=MS.gene09188.t1.exon3;Parent=MS.gene09188.t1
chr4.2 CDS 14998820 14998989 14998820 ID=cds.MS.gene09188.t1;Parent=MS.gene09188.t1
chr4.2 exon 14999103 14999283 14999103 ID=MS.gene09188.t1.exon4;Parent=MS.gene09188.t1
chr4.2 CDS 14999103 14999283 14999103 ID=cds.MS.gene09188.t1;Parent=MS.gene09188.t1
chr4.2 exon 14999395 14999575 14999395 ID=MS.gene09188.t1.exon5;Parent=MS.gene09188.t1
chr4.2 CDS 14999395 14999575 14999395 ID=cds.MS.gene09188.t1;Parent=MS.gene09188.t1
chr4.2 exon 14999664 14999763 14999664 ID=MS.gene09188.t1.exon6;Parent=MS.gene09188.t1
chr4.2 CDS 14999664 14999763 14999664 ID=cds.MS.gene09188.t1;Parent=MS.gene09188.t1
chr4.2 exon 15000601 15000728 15000601 ID=MS.gene09188.t1.exon7;Parent=MS.gene09188.t1
chr4.2 CDS 15000601 15000728 15000601 ID=cds.MS.gene09188.t1;Parent=MS.gene09188.t1
chr4.2 exon 15000856 15000864 15000856 ID=MS.gene09188.t1.exon8;Parent=MS.gene09188.t1
chr4.2 CDS 15000856 15000864 15000856 ID=cds.MS.gene09188.t1;Parent=MS.gene09188.t1
Gene Sequence

>MS.gene09188

ATGGGTTCATCTGGTTTTGTCCTTCCAGACATTTACATCCCACAACAATGGTCACAAGCTGCTAACTCCATTACATCCAGTCCAACTCCTCCCATCACATTTGTTTGTGGAGCAAGTAACTGCGGCAAATCAACCTTCTCGCGAAATCTCCTCAATGTCCTATTAACTAGATGCGACCGAGTTGCTTATCTTGAAACTGATGTTGGCCAACCTGAGTTTACTCCTCCTGGTTTTATCTCTCTTACCATTGTTAATAAAGTTACTCCAGGTAGGTTTTCTAACATATACCTATTCTAACATTTACATGTAATAGAATCACAAGTTAAATAAGTTTTCCGTCGTACATAATTATCAGCTTTCGTTTTAATGTTCTCGTAAAAATTGTTGCATGTTTGTGTCTAATAAAATTCCCACATTTAGTTTTACTACGTGCTAAATTCAAATTTGTTTTTTAAATTTAAAAGTTTAAGGAAAATTAAACAAATTTGAATTTAACATGAATTGAAACCACGTGCTTGAAAATTTTAAACAGACGCAAACTTGTCACTTTTTTCATGGGAGGAAGAAACGAGATTTGATAATTATGTTGGGACAACAATCATATTTAACCCTATATTCATTGATTCAATAATTTTCTATAAAAATGTTCATTATGGATATTGACACGAGTTTTTTTTGTGTTTTTACTATGTTAGATTTGACGATTCCATGCCTGAAAACACCAGAGAGGTATAAACTATGAAATGTTATCTCATATCTTGATTAACTTTGTACTAATGATTAGTTAACTTTATACTAATGATGTAAATCAAATATTTTGAAACCACGAGAGATGTATAAACTATGAAATTTGATGTTGCCTCAACCTTAACTATTGTATCTTGTTAAATAATCAATTCTTGACTTGACCAATGGTCTCAGACTCAATTACTAGTTTAGATATTGTCAGATTCTTAATAGCATAGATTTGAGGCTTTTGCCACTCACATAGATAAAAGAATTGTGAATAGTTTACATGCCTTAAGATACTTTCGATCAGAAATTTTCGCAACAGTGCTAGTTTCAGTATCAATACCAGTTTGTATCAATGTCATTAACATGTGATACTGGCACTCATGATATCTCATCTCAATAATTTGGGAACTGAGACTGTCTTTTTGGTTTTTTATTTGTTGCTTTGAATTTGAGGTCCTATTTTGTCACATTGTTATGCAGTCTTCTACAAACAGTGAGTTGGATGAAATTTTGTCTGTTTGTAGTTTGTAGGATATATTTGATTCTTTTAGTGTCGGCCAATCCATGTCTTATTTGAATGAATGCGCTCACTTGTAATGGAGTTGCTTGACTCACTTTCTGTCGTTTATTGCGTAACAGATCCCTTTTTTTTGGTGATGTTTCTTCTCAGAAAGATCCGTCAACATACTTAAAATACATATGTTCTATTTATGATTATTATCATAAGGAGTATTGCACCTTTGATAAGAGACAAAATTCTTCTAAGATTCACTTGCCTCTTGTAGTGAATACCTCTGGCTGGGTTAAAGGTAAACTCGTATATTTTGTATTTTTACTCCAAATAAACTACTGCTTTTCTCGACCAATAACAATATATCAATTATTATGTTACTCACTCAATGTGTTTTGTCTTGCATTGCAGGTGATGGTTATAAGGTATTAGTGAACATGCTAAAATATATTGGTCCCACTCATATAGTTAAGATAGGCATATCCACTGATCTGAAGAATTTACCTGGAAAGTTCGGGTCAAATGGGAAGAATAACAGAAGAGTTAAATTGATTGAGATATATCCAGCTCGTCAGGACTCATTAAATAAATCGTAAGCACAAATAATATAAGTTACCATTTTCAGCTGCTAGTTTAGTTCATTATATTTTAGACTTAATTTAAGTATTACAGAATAACAGAATATCCCTATTTGTTTCCCCAGGGTGCCTGCTCAAAAGGATGCTCGTCTTGTGCGCGATTCACGAATAATAGCTTATTTCAGACAGTGCTTTCCTAGTGATTCAGATATTTCAACAATCAAGGATCTTGCAAATTCCTTGACCTCTCACTGTCCTTATAATGTTCCCATCGCAAGCATAAAGATTCGACGTGTTCTTCACGAGGTTAGTTCTAATGCTACTTATCATCTCTTCTGGTTCTGTCTCATTCATTGATCAAAACATTTTACATGACTTATGAATATTTTTGTAGGTCCCAATCTCTGAAATACTCTCCAGCCTGAATGCTAGTATTGTTGGCTTAGCAGTTGAATCTGAAGGACCTAAAAAGTTACCCTGGTGCCTTGGTCTTGGTAGGTAACTTGGTTGACTTCCAAATTCCAAAATGTCTCGTCATAGGTTGATTTCTCAAAATTTAAAAGAATATGAATTATTTATTCATCCTAAACAAAACACCAATGAGTGTTCTATGTACATGAGACCCTAAGGCAGAACAAGAAACTAATTAATGAATAATGACTAAATCCGATGATGTTTGAGATTCCCTAAGCCACTAACGAGTCCTCCAAATGATTCAACTCTATGAATAAAGTTACAAAATAAAGGATATTAGTTACATACACAGGACTATATCTGTTATAGAAACAAACATGCTGACGATCCCCCTCAAGCTGATTAATAGGTATTTACTTTTCCCAGCTTGTATACAATATTCTGGAACATGGTCCTGCTTGGCCCTTTGGCAGTAATGTGGTCAACTAGTTGAAGGCCCCCTATTTTGCGGCCTTCGTGTAGAAGGCTTCCAATGCCTGTAAACAAAAAACAACATTTGGATTATAATGAAAAGATAAATTTAAATTTTGAACTTGATATCATTCAAACTTATGGTAGCAGTGAGGTACACTAAAAGGAATTTGCATTTGTAGGTTGACTTCATTATATCTCATATTTCTGTTGGCTGTAACAGGAGTATCATGAGTTGCAACACATCTTAATGGATAATTACCTTTTTAATGTATCAAACATCCACAAATAGAAAAGATAAAGAATAAATTCATATTCCTCTGGTCTTCTGATCTTGTAATGACGAGCACATGGATCATACAGCCATCTAATTTTCTGGCTGTCATGCATTTGAGTAATTCTTAGCATCATTACTGTTAAAGGCGTTTTGATTTCTTGTGATTCAGGTATTGTGAGGGGCATTGACACAGTTAAAGGCATGCTCTATCTGATTACACCTGTGCCATTTGGTTCTCTGAAAAAAGTCAACCTCTTGCTTCAGGGTAATATCCAAATTCCTACAAGTTTATTGCAGGTAAGAAATTGTTTTATGTACTTGCTCTTTCTTCTGAACATTAACTTGTAGAAATAATTCTTGTAAAAAAAGTTAACTTTATTACTTTGAGCTACCAAAGGAAAACTTTCTGTTGGTTTTTCCTTAGGTTACATGA
Protein sequence

>MS.gene09188.t1

MGSSGFVLPDIYIPQQWSQAANSITSSPTPPITFVCGASNCGKSTFSRNLLNVLLTRCDRVAYLETDVGQPEFTPPGFISLTIVNKVTPDLTIPCLKTPERSLFFGDVSSQKDPSTYLKYICSIYDYYHKEYCTFDKRQNSSKIHLPLVVNTSGWVKGDGYKVLVNMLKYIGPTHIVKIGISTDLKNLPGKFGSNGKNNRRVKLIEIYPARQDSLNKSVPAQKDARLVRDSRIIAYFRQCFPSDSDISTIKDLANSLTSHCPYNVPIASIKIRRVLHEVPISEILSSLNASIVGLAVESEGPKKLPWCLGLGIVRGIDTVKGMLYLITPVPFGSLKKVNLLLQGNIQIPTSLLQVT