AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene01449

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01449.t1 MTR_2g014030 100.000 248 0 0 1 248 1 248 0.0 501
MS.gene01449.t1 MTR_4g088595 96.774 248 8 0 1 248 1 248 2.02e-176 485
MS.gene01449.t1 MTR_7g053160 95.968 248 10 0 1 248 1 248 5.74e-175 481
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01449.t1 AT5G10360 92.562 242 18 0 1 242 1 242 1.63e-163 452
MS.gene01449.t1 AT4G31700 92.149 242 19 0 1 242 1 242 4.08e-155 431
MS.gene01449.t1 AT5G10360 73.967 242 11 1 1 242 1 190 1.18e-118 337
MS.gene01449.t1 AT4G31700 91.667 180 15 0 63 242 1 180 1.14e-106 306

Find 70 sgRNAs with CRISPR-Local

Find 232 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGAAGAGCTTACGGATTT+TGG 0.153100 2.2:+68775827 None:intergenic
TTGCTACTCGCAGGAATTTA+AGG 0.172205 2.2:-68776988 MS.gene01449:intron
TAAATTGTAGTCGTGCATTT+TGG 0.186966 2.2:-68777133 MS.gene01449:intron
CTCTTAGGACCCCTCATTCT+TGG 0.233777 2.2:+68775855 None:intergenic
GAGGATCTCACAAGAGGTTT+TGG 0.240752 2.2:-68777105 MS.gene01449:CDS
CATCGATTTCAAGCTTCTTC+TGG 0.244242 2.2:+68777259 None:intergenic
GTGACCAGGCGCTGTATCTT+TGG 0.280234 2.2:+68774638 None:intergenic
TGACCAGGCGCTGTATCTTT+GGG 0.285753 2.2:+68774639 None:intergenic
GTTAACACACCCTGCTTCAT+TGG 0.338665 2.2:+68776921 None:intergenic
GGAACGCCGCAGTGAGAGTT+TGG 0.366197 2.2:-68774495 MS.gene01449:CDS
TGCTACTCGCAGGAATTTAA+GGG 0.399045 2.2:-68776987 MS.gene01449:intron
AGACCTCTCTGTTCTGAACT+TGG 0.408965 2.2:-68775931 MS.gene01449:CDS
ACTTCTTGCCTCTAGATTGA+AGG 0.416460 2.2:-68774525 MS.gene01449:CDS
TGGAAAGATTGAAGAGCTTA+CGG 0.437945 2.2:+68775820 None:intergenic
CTCTTTCTTTGAAGAGTCAA+AGG 0.440470 2.2:+68774614 None:intergenic
AAGTTCAGAACAGAGAGGTC+TGG 0.450666 2.2:+68775933 None:intergenic
CGCTGCAACACCTTGCTTAG+CGG 0.459636 2.2:+68774448 None:intergenic
GGCGCAATGGAGAGCGAAGA+AGG 0.461619 2.2:-68775983 MS.gene01449:CDS
CTTTGACTCTTCAAAGAAAG+AGG 0.462251 2.2:-68774613 MS.gene01449:CDS
CTTCCTGACATCATCATCCT+TGG 0.467618 2.2:+68775800 None:intergenic
AGCAAGAGGCGAACACGACC+AGG 0.471596 2.2:+68776897 None:intergenic
ACAAGGATTTCCAATGAAGC+AGG 0.480359 2.2:-68776931 MS.gene01449:CDS
GAACTTGGTTATTGTGAAGA+AGG 0.484415 2.2:-68775916 MS.gene01449:CDS
AAGGGTGAAAATGATTTGCC+TGG 0.498405 2.2:-68775897 MS.gene01449:CDS
GGATATGTCTTCAAAATCAC+TGG 0.501460 2.2:-68776966 MS.gene01449:CDS
AGTTCAGAACAGAGAGGTCT+GGG 0.504245 2.2:+68775934 None:intergenic
TTTCCAAGGATGATGATGTC+AGG 0.517231 2.2:-68775803 MS.gene01449:CDS
GACCAGGCGCTGTATCTTTG+GGG 0.517909 2.2:+68774640 None:intergenic
TTCTTCTGGCAACCAGTGGT+TGG 0.519589 2.2:+68777273 None:intergenic
AACGTTGCAAATCCAACCAC+TGG 0.533153 2.2:-68777285 MS.gene01449:CDS
GTTTGGCTAAGAAGAGATCA+AGG 0.534691 2.2:-68774478 MS.gene01449:CDS
ATGTTGAAAAGCCAAGAATG+AGG 0.544680 2.2:-68775866 MS.gene01449:CDS
CTTCCGTGTCCACGGAAACA+AGG 0.545510 2.2:+68776005 None:intergenic
AGCCCCAAAGATACAGCGCC+TGG 0.547274 2.2:-68774642 MS.gene01449:CDS
AAGCTTCTTCTGGCAACCAG+TGG 0.547317 2.2:+68777269 None:intergenic
CTTGTTTCCGTGGACACGGA+AGG 0.556226 2.2:-68776004 MS.gene01449:CDS
CGGACTTTCACAACCAAAGC+AGG 0.557230 2.2:-68775759 MS.gene01449:intron
CGAAGAAGGAAGTCTGTTCG+TGG 0.557458 2.2:-68775969 MS.gene01449:CDS
CTCTAGATTGAAGGAGCAGA+GGG 0.562368 2.2:-68774516 MS.gene01449:CDS
CGTGGACACGGAAGGCGCAA+TGG 0.565614 2.2:-68775996 MS.gene01449:CDS
AAGCAGGGTGTGTTAACCCC+TGG 0.565983 2.2:-68776915 MS.gene01449:CDS
TGTTGAAAAGCCAAGAATGA+GGG 0.570225 2.2:-68775865 MS.gene01449:CDS
ACTCCTTGTTTCCGTGGACA+CGG 0.572387 2.2:-68776008 MS.gene01449:CDS
GCAGGTACTCCTTGTTTCCG+TGG 0.573410 2.2:-68776014 MS.gene01449:intron
TTTGACTCTTCAAAGAAAGA+GGG 0.574504 2.2:-68774612 MS.gene01449:CDS
TAAGCTCTTCAATCTTTCCA+AGG 0.582836 2.2:-68775817 MS.gene01449:CDS
CATTGCGCCTTCCGTGTCCA+CGG 0.591891 2.2:+68775997 None:intergenic
AACTTGGTTATTGTGAAGAA+GGG 0.597761 2.2:-68775915 MS.gene01449:CDS
GAAGAGTCAAAGGAGTGACC+AGG 0.604666 2.2:+68774624 None:intergenic
TTATACCTCTGTGGAGCAAG+AGG 0.605499 2.2:+68776883 None:intergenic
CTTTGGTTGTGAAAGTCCGA+CGG 0.609736 2.2:+68775763 None:intergenic
AGGCTCTCCACCGCTAAGCA+AGG 0.611423 2.2:-68774458 MS.gene01449:CDS
TATGTCTTCAAAATCACTGG+TGG 0.614527 2.2:-68776963 MS.gene01449:CDS
GGACAAGAGGATCTCACAAG+AGG 0.614846 2.2:-68777111 MS.gene01449:CDS
GCAAGAGGCGAACACGACCA+GGG 0.615545 2.2:+68776898 None:intergenic
GGTTGTGAAAGTCCGACGGT+AGG 0.616762 2.2:+68775767 None:intergenic
TGCAACACCTTGCTTAGCGG+TGG 0.616816 2.2:+68774451 None:intergenic
CCTCTAGATTGAAGGAGCAG+AGG 0.621309 2.2:-68774517 MS.gene01449:CDS
CCTCTGCTCCTTCAATCTAG+AGG 0.626623 2.2:+68774517 None:intergenic
GTTGAAAAGCCAAGAATGAG+GGG 0.636999 2.2:-68775864 MS.gene01449:CDS
CAAGGATTTCCAATGAAGCA+GGG 0.641279 2.2:-68776930 MS.gene01449:CDS
GAAGAAGGAAGTCTGTTCGT+GGG 0.645378 2.2:-68775968 MS.gene01449:CDS
GTAGAAAGGTTATACCTCTG+TGG 0.668019 2.2:+68776874 None:intergenic
AATTGCCAAAGCAAAATCAG+AGG 0.670645 2.2:-68774564 MS.gene01449:CDS
CAAGAGGCGAACACGACCAG+GGG 0.676279 2.2:+68776899 None:intergenic
ACTGGTGGATGTGACAAACA+AGG 0.694989 2.2:-68776948 MS.gene01449:CDS
AGTATGTCAACACCTACCGT+CGG 0.698935 2.2:-68775779 MS.gene01449:CDS
GTTCGCCTCTTGCTCCACAG+AGG 0.710024 2.2:-68776888 MS.gene01449:intron
TCTTAGCCAAACTCTCACTG+CGG 0.768400 2.2:+68774489 None:intergenic
TAACCAAGTTCAGAACAGAG+AGG 0.775773 2.2:+68775928 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATATATATATAAAT+TGG + chr2.2:68775531-68775550 None:intergenic 0.0%
!! ATAATTAATATACAAAACAA+AGG + chr2.2:68776544-68776563 None:intergenic 10.0%
!! CAAAGAAAAAAAAAAAAAAA+AGG + chr2.2:68774613-68774632 None:intergenic 10.0%
!! TCAATATAACAATTAATTTA+AGG + chr2.2:68774793-68774812 None:intergenic 10.0%
!!! TTTTATTAATTATTATAGTG+AGG - chr2.2:68774994-68775013 MS.gene01449:intron 10.0%
!! AAAAAAAAAAAACTGTAGAA+AGG + chr2.2:68774964-68774983 None:intergenic 15.0%
!! ATTTATTAATTATGTTGTTC+GGG - chr2.2:68775462-68775481 MS.gene01449:intron 15.0%
!! CATAATTAACAAAACTAAAA+AGG + chr2.2:68775418-68775437 None:intergenic 15.0%
!! GATTTATTAATTATGTTGTT+CGG - chr2.2:68775461-68775480 MS.gene01449:intron 15.0%
!! TATGGATCTATAATTTATAA+TGG - chr2.2:68777103-68777122 MS.gene01449:CDS 15.0%
!! TTGTAATAGAATATTATCTA+TGG - chr2.2:68777073-68777092 MS.gene01449:intron 15.0%
!!! AAAAAAAGATCTAAAATAGA+GGG + chr2.2:68775295-68775314 None:intergenic 15.0%
!! AAAAATTCCAACTTAGATAT+TGG + chr2.2:68776192-68776211 None:intergenic 20.0%
!! ACCAAATAAATACTTAACAA+GGG + chr2.2:68775762-68775781 None:intergenic 20.0%
!! AGATTTAAAAATAGAATCAG+AGG + chr2.2:68776842-68776861 None:intergenic 20.0%
!! ATATTAATTATGCTGTGTTA+TGG - chr2.2:68776553-68776572 MS.gene01449:intron 20.0%
!! CAAACAGTAATAAAAACAAA+AGG + chr2.2:68775233-68775252 None:intergenic 20.0%
!! GATTTAAAAATAGAATCAGA+GGG + chr2.2:68776841-68776860 None:intergenic 20.0%
!! TACCAAATAAATACTTAACA+AGG + chr2.2:68775763-68775782 None:intergenic 20.0%
!! TCAAGTGATAAATACTAAAT+AGG + chr2.2:68776157-68776176 None:intergenic 20.0%
!! TGTACTATTATTTCCATTAT+AGG - chr2.2:68775173-68775192 MS.gene01449:intron 20.0%
!! TGTATGTATTGTTAGTTATA+GGG - chr2.2:68776283-68776302 MS.gene01449:intron 20.0%
!! TTAGTTAGTTAGTTAACATT+GGG - chr2.2:68776624-68776643 MS.gene01449:intron 20.0%
!!! ATCACTTGCTATTATAATAT+AGG - chr2.2:68776216-68776235 MS.gene01449:intron 20.0%
!!! GAAAAAAAGATCTAAAATAG+AGG + chr2.2:68775296-68775315 None:intergenic 20.0%
!!! GAATTTATTTTGTATGTTAG+TGG - chr2.2:68775339-68775358 MS.gene01449:intron 20.0%
!!! TAGATCTTTTTTTCACTAAT+AGG - chr2.2:68775302-68775321 MS.gene01449:intron 20.0%
!!! TCACTTGCTATTATAATATA+GGG - chr2.2:68776217-68776236 MS.gene01449:intron 20.0%
! AATTTAATGTTTGTTTGAGC+AGG - chr2.2:68775789-68775808 MS.gene01449:CDS 25.0%
! ACAAAATAAATTCACCAGTT+TGG + chr2.2:68775332-68775351 None:intergenic 25.0%
! ACTGCAAAACAATTTCTATA+TGG + chr2.2:68774514-68774533 None:intergenic 25.0%
! ATTATCTATGGTCATAACTA+TGG - chr2.2:68777085-68777104 MS.gene01449:intron 25.0%
! CAAAATAAATTCACCAGTTT+GGG + chr2.2:68775331-68775350 None:intergenic 25.0%
! CTCTTTATCATTTACTTGAA+TGG - chr2.2:68775205-68775224 MS.gene01449:intron 25.0%
! CTGTATGTATTGTTAGTTAT+AGG - chr2.2:68776282-68776301 MS.gene01449:intron 25.0%
! GTTAGTTAGTTAGTTAACAT+TGG - chr2.2:68776623-68776642 MS.gene01449:intron 25.0%
!! AAAATATCCGCTCTTTTATT+TGG - chr2.2:68776985-68777004 MS.gene01449:intron 25.0%
!! TAGCTTCTTTCAATTTTTCA+AGG + chr2.2:68776470-68776489 None:intergenic 25.0%
!! TGTCAATTTGCAATTGTTTT+GGG + chr2.2:68776865-68776884 None:intergenic 25.0%
!! TTGTCAATTTGCAATTGTTT+TGG + chr2.2:68776866-68776885 None:intergenic 25.0%
!!! AAATATCCGCTCTTTTATTT+GGG - chr2.2:68776986-68777005 MS.gene01449:intron 25.0%
!!! AACAAAGGTTTTAAGTTTAG+TGG + chr2.2:68776529-68776548 None:intergenic 25.0%
!!! ACAAAGGTTTTAAGTTTAGT+GGG + chr2.2:68776528-68776547 None:intergenic 25.0%
!!! ATAGCTTTGGTTTTGAAAAA+TGG - chr2.2:68776118-68776137 MS.gene01449:intron 25.0%
!!! GAATATTTAGTTGTTTTGAG+AGG - chr2.2:68776692-68776711 MS.gene01449:intron 25.0%
!!! TATGTTAGTGGATTTTTTGT+AGG - chr2.2:68775351-68775370 MS.gene01449:intron 25.0%
!!! TCAATTTTTCAAGGATTGAT+GGG + chr2.2:68776461-68776480 None:intergenic 25.0%
!!! TTCAATTTTTCAAGGATTGA+TGG + chr2.2:68776462-68776481 None:intergenic 25.0%
AACTTGGTTATTGTGAAGAA+GGG - chr2.2:68775906-68775925 MS.gene01449:CDS 30.0%
AATAATTGTCGTAAGTTAGC+TGG + chr2.2:68776950-68776969 None:intergenic 30.0%
ACTTACGACAATTATTACAG+CGG - chr2.2:68776954-68776973 MS.gene01449:CDS 30.0%
AGAGTATACAACACCTATAA+TGG + chr2.2:68775189-68775208 None:intergenic 30.0%
AGATTGCTAAATAGATGAAG+AGG - chr2.2:68776744-68776763 MS.gene01449:intron 30.0%
ATTATAATATAGGGTGAGTC+TGG - chr2.2:68776226-68776245 MS.gene01449:intron 30.0%
ATTGTCTCCAATATCTAAGT+TGG - chr2.2:68776182-68776201 MS.gene01449:intron 30.0%
ATTTGGGGGAAAAACATAAA+TGG + chr2.2:68775498-68775517 None:intergenic 30.0%
CAATGAAATCAAAGGAACAA+GGG + chr2.2:68774477-68774496 None:intergenic 30.0%
CACAAACTCATCTATACAAT+GGG + chr2.2:68776401-68776420 None:intergenic 30.0%
CATCTATTTAGCAATCTATC+AGG + chr2.2:68776741-68776760 None:intergenic 30.0%
CTAAATTAGCCCATCTAAAT+TGG + chr2.2:68775131-68775150 None:intergenic 30.0%
GATAAGAGCAATGAAATCAA+AGG + chr2.2:68774485-68774504 None:intergenic 30.0%
GATTGCTAAATAGATGAAGA+GGG - chr2.2:68776745-68776764 MS.gene01449:intron 30.0%
GCCCTTGTTAAGTATTTATT+TGG - chr2.2:68775758-68775777 MS.gene01449:intron 30.0%
GGAAAGTAGTTTAGTAATTG+CGG - chr2.2:68776656-68776675 MS.gene01449:intron 30.0%
TACTATAAACCCATTTGTGT+TGG - chr2.2:68775043-68775062 MS.gene01449:intron 30.0%
TATTAATTATGTTGTTCGGG+TGG - chr2.2:68775465-68775484 MS.gene01449:intron 30.0%
TGTATTATCAACAGAGAATC+AGG + chr2.2:68776099-68776118 None:intergenic 30.0%
TGTTTGAATTATTCGTTTCC+TGG - chr2.2:68775247-68775266 MS.gene01449:intron 30.0%
TTAGTTAGTTAACATTGGGA+AGG - chr2.2:68776628-68776647 MS.gene01449:intron 30.0%
TTTATAATACCAGCGAGAAT+GGG - chr2.2:68775613-68775632 MS.gene01449:intron 30.0%
TTTGACTCTTCAAAGAAAGA+GGG - chr2.2:68777209-68777228 MS.gene01449:intron 30.0%
! AAATTGTAGTCGTGCATTTT+GGG - chr2.2:68774689-68774708 MS.gene01449:intron 30.0%
! TAAATTGTAGTCGTGCATTT+TGG - chr2.2:68774688-68774707 MS.gene01449:intron 30.0%
! TCTAAAATAGAGGGTTAAAC+CGG + chr2.2:68775286-68775305 None:intergenic 30.0%
! TGTGTTATGGTTAGAAGTAT+AGG - chr2.2:68776566-68776585 MS.gene01449:intron 30.0%
! TTTTATAATACCAGCGAGAA+TGG - chr2.2:68775612-68775631 MS.gene01449:intron 30.0%
!! CTGTTGATAATACATAGCTT+TGG - chr2.2:68776105-68776124 MS.gene01449:intron 30.0%
!!! TTGGTTTTGAAAAATGGTCT+TGG - chr2.2:68776124-68776143 MS.gene01449:intron 30.0%
AAATTGGCAGCTGACAAATT+TGG + chr2.2:68775515-68775534 None:intergenic 35.0%
AACAAGGGAAATTAGGGTTT+GGG + chr2.2:68774462-68774481 None:intergenic 35.0%
AAGGAACCCAAATAAAAGAG+CGG + chr2.2:68776995-68777014 None:intergenic 35.0%
AATTGCCAAAGCAAAATCAG+AGG - chr2.2:68777257-68777276 MS.gene01449:CDS 35.0%
AATTGGCAGCTGACAAATTT+GGG + chr2.2:68775514-68775533 None:intergenic 35.0%
ACAAGAACTGAATTGCAACA+GGG - chr2.2:68776811-68776830 MS.gene01449:intron 35.0%
AGTACGAAATTAGTTGAGAG+TGG + chr2.2:68776926-68776945 None:intergenic 35.0%
ATGTTGAAAAGCCAAGAATG+AGG - chr2.2:68775955-68775974 MS.gene01449:CDS 35.0%
CAAAGGAACAAGGGAAATTA+GGG + chr2.2:68774468-68774487 None:intergenic 35.0%
CCACAAACTCATCTATACAA+TGG + chr2.2:68776402-68776421 None:intergenic 35.0%
CCATTGTATAGATGAGTTTG+TGG - chr2.2:68776399-68776418 MS.gene01449:intron 35.0%
CTAGTTCCTATTAACAGCAA+CGG + chr2.2:68776777-68776796 None:intergenic 35.0%
CTTTGACTCTTCAAAGAAAG+AGG - chr2.2:68777208-68777227 MS.gene01449:intron 35.0%
GAACTTGGTTATTGTGAAGA+AGG - chr2.2:68775905-68775924 MS.gene01449:CDS 35.0%
GCAATGAAATCAAAGGAACA+AGG + chr2.2:68774478-68774497 None:intergenic 35.0%
GGATATGTCTTCAAAATCAC+TGG - chr2.2:68774855-68774874 MS.gene01449:intron 35.0%
TAAGCTCTTCAATCTTTCCA+AGG - chr2.2:68776004-68776023 MS.gene01449:CDS 35.0%
TAGTTCCTATTAACAGCAAC+GGG + chr2.2:68776776-68776795 None:intergenic 35.0%
TATGTCTTCAAAATCACTGG+TGG - chr2.2:68774858-68774877 MS.gene01449:intron 35.0%
TCAAAGGAACAAGGGAAATT+AGG + chr2.2:68774469-68774488 None:intergenic 35.0%
TGGAAAGATTGAAGAGCTTA+CGG + chr2.2:68776004-68776023 None:intergenic 35.0%
TGTTGAAAAGCCAAGAATGA+GGG - chr2.2:68775956-68775975 MS.gene01449:CDS 35.0%
TTATAATACCAGCGAGAATG+GGG - chr2.2:68775614-68775633 MS.gene01449:intron 35.0%
! ATATTCCCGTTGCTGTTAAT+AGG - chr2.2:68776768-68776787 MS.gene01449:intron 35.0%
! ATCTAGAGGCAAGAAGTTTT+TGG + chr2.2:68777293-68777312 None:intergenic 35.0%
! ATTGAAGAGCTTACGGATTT+TGG + chr2.2:68775997-68776016 None:intergenic 35.0%
! CTCTTTCTTTGAAGAGTCAA+AGG + chr2.2:68777210-68777229 None:intergenic 35.0%
! GGAAGAAATTTTACCTGCTT+TGG + chr2.2:68776078-68776097 None:intergenic 35.0%
! TTTTCAACATCAGTCAAACC+AGG + chr2.2:68775945-68775964 None:intergenic 35.0%
!! TTTTCAAGGATTGATGGGAA+GGG + chr2.2:68776456-68776475 None:intergenic 35.0%
!!! AGGTTGACTTGTTTTATCAG+AGG - chr2.2:68777043-68777062 MS.gene01449:intron 35.0%
!!! AGGTTTTAAGTTTAGTGGGA+AGG + chr2.2:68776524-68776543 None:intergenic 35.0%
!!! GGTTTTAAGTTTAGTGGGAA+GGG + chr2.2:68776523-68776542 None:intergenic 35.0%
!!! TGTTTTGAGAGGAAGATTTC+AGG - chr2.2:68776703-68776722 MS.gene01449:intron 35.0%
!!! TTTTTCAAGGATTGATGGGA+AGG + chr2.2:68776457-68776476 None:intergenic 35.0%
AACACGTATCTGATTGTGCA+GGG - chr2.2:68777142-68777161 MS.gene01449:intron 40.0%
ACAAGGATTTCCAATGAAGC+AGG - chr2.2:68774890-68774909 MS.gene01449:intron 40.0%
ACAAGGGAAATTAGGGTTTG+GGG + chr2.2:68774461-68774480 None:intergenic 40.0%
ACGACAATTATTACAGCGGT+CGG - chr2.2:68776958-68776977 MS.gene01449:CDS 40.0%
ACTTCTTGCCTCTAGATTGA+AGG - chr2.2:68777296-68777315 MS.gene01449:intron 40.0%
AGATTCAAACCGAAACCTCA+AGG + chr2.2:68775100-68775119 None:intergenic 40.0%
AGCAATTAACACGTCAAAGG+AGG + chr2.2:68775683-68775702 None:intergenic 40.0%
ATTGGCAGCTGACAAATTTG+GGG + chr2.2:68775513-68775532 None:intergenic 40.0%
CAAGGATTTCCAATGAAGCA+GGG - chr2.2:68774891-68774910 MS.gene01449:intron 40.0%
CAATTAACACGTCAAAGGAG+GGG + chr2.2:68775681-68775700 None:intergenic 40.0%
CACAAGAACTGAATTGCAAC+AGG - chr2.2:68776810-68776829 MS.gene01449:intron 40.0%
CCATCATCACCAACACAAAT+GGG + chr2.2:68775055-68775074 None:intergenic 40.0%
CTTTCATGGACTTGCATGAT+AGG - chr2.2:68777020-68777039 MS.gene01449:intron 40.0%
GAACAAGGGAAATTAGGGTT+TGG + chr2.2:68774463-68774482 None:intergenic 40.0%
GAACCATCTACCATTAGAGA+CGG + chr2.2:68775721-68775740 None:intergenic 40.0%
GCAATTAACACGTCAAAGGA+GGG + chr2.2:68775682-68775701 None:intergenic 40.0%
GTAGAAAGGTTATACCTCTG+TGG + chr2.2:68774950-68774969 None:intergenic 40.0%
GTCAGCAATTAACACGTCAA+AGG + chr2.2:68775686-68775705 None:intergenic 40.0%
GTTAACATTGGGAAGGTAGT+TGG - chr2.2:68776635-68776654 MS.gene01449:intron 40.0%
GTTGAAAAGCCAAGAATGAG+GGG - chr2.2:68775957-68775976 MS.gene01449:CDS 40.0%
GTTTGGCTAAGAAGAGATCA+AGG - chr2.2:68777343-68777362 MS.gene01449:intron 40.0%
TAACACGTATCTGATTGTGC+AGG - chr2.2:68777141-68777160 MS.gene01449:intron 40.0%
TAACCAAGTTCAGAACAGAG+AGG + chr2.2:68775896-68775915 None:intergenic 40.0%
TAATATAGGGTGAGTCTGGT+TGG - chr2.2:68776230-68776249 MS.gene01449:intron 40.0%
TAGCCCATCTAAATTGGAAC+CGG + chr2.2:68775125-68775144 None:intergenic 40.0%
TCTCCGGTTCCAATTTAGAT+GGG - chr2.2:68775119-68775138 MS.gene01449:intron 40.0%
TGCTACTCGCAGGAATTTAA+GGG - chr2.2:68774834-68774853 MS.gene01449:intron 40.0%
TGTTAGTTATAGGGTGAGTC+TGG - chr2.2:68776292-68776311 MS.gene01449:intron 40.0%
TTGCTACTCGCAGGAATTTA+AGG - chr2.2:68774833-68774852 MS.gene01449:intron 40.0%
! AAGGGTGAAAATGATTTGCC+TGG - chr2.2:68775924-68775943 MS.gene01449:CDS 40.0%
! AGGATCTCACAAGAGGTTTT+GGG - chr2.2:68774717-68774736 MS.gene01449:intron 40.0%
! TTTCCAAGGATGATGATGTC+AGG - chr2.2:68776018-68776037 MS.gene01449:intron 40.0%
!! CATCGATTTCAAGCTTCTTC+TGG + chr2.2:68774565-68774584 None:intergenic 40.0%
AACGTTGCAAATCCAACCAC+TGG - chr2.2:68774536-68774555 MS.gene01449:CDS 45.0%
AAGTTCAGAACAGAGAGGTC+TGG + chr2.2:68775891-68775910 None:intergenic 45.0%
AGTATGTCAACACCTACCGT+CGG - chr2.2:68776042-68776061 MS.gene01449:intron 45.0%
AGTTATAGGGTGAGTCTGGT+TGG - chr2.2:68776296-68776315 MS.gene01449:intron 45.0%
AGTTCAGAACAGAGAGGTCT+GGG + chr2.2:68775890-68775909 None:intergenic 45.0%
CAAGGGAAATTAGGGTTTGG+GGG + chr2.2:68774460-68774479 None:intergenic 45.0%
CAAGTCCATGAAAGCAGGAA+AGG + chr2.2:68777014-68777033 None:intergenic 45.0%
CACTAATAGGAGACCCAAAC+TGG - chr2.2:68775315-68775334 MS.gene01449:intron 45.0%
CTCTAGATTGAAGGAGCAGA+GGG - chr2.2:68777305-68777324 MS.gene01449:intron 45.0%
CTCTCCGGTTCCAATTTAGA+TGG - chr2.2:68775118-68775137 MS.gene01449:intron 45.0%
CTTCCTGACATCATCATCCT+TGG + chr2.2:68776024-68776043 None:intergenic 45.0%
CTTTGGTTGTGAAAGTCCGA+CGG + chr2.2:68776061-68776080 None:intergenic 45.0%
GAAGAAGGAAGTCTGTTCGT+GGG - chr2.2:68775853-68775872 MS.gene01449:CDS 45.0%
GCCATCATCACCAACACAAA+TGG + chr2.2:68775056-68775075 None:intergenic 45.0%
GTTAACACACCCTGCTTCAT+TGG + chr2.2:68774903-68774922 None:intergenic 45.0%
TACCATTAGAGACGGCAAAG+TGG + chr2.2:68775713-68775732 None:intergenic 45.0%
TAGTGGGAAGGGATAGAAGT+CGG + chr2.2:68776512-68776531 None:intergenic 45.0%
TCATGCAAGTCCATGAAAGC+AGG + chr2.2:68777019-68777038 None:intergenic 45.0%
TCATGGACTTGCATGATAGG+AGG - chr2.2:68777023-68777042 MS.gene01449:intron 45.0%
TCTTAGCCAAACTCTCACTG+CGG + chr2.2:68777335-68777354 None:intergenic 45.0%
TGACGTGTTAATTGCTGACC+TGG - chr2.2:68775687-68775706 MS.gene01449:intron 45.0%
TTATACCTCTGTGGAGCAAG+AGG + chr2.2:68774941-68774960 None:intergenic 45.0%
TTCAAACCGAAACCTCAAGG+AGG + chr2.2:68775097-68775116 None:intergenic 45.0%
TTGCCGTCTCTAATGGTAGA+TGG - chr2.2:68775715-68775734 MS.gene01449:intron 45.0%
TTGGCAGCTGACAAATTTGG+GGG + chr2.2:68775512-68775531 None:intergenic 45.0%
! ACTGGTGGATGTGACAAACA+AGG - chr2.2:68774873-68774892 MS.gene01449:intron 45.0%
! AGACCTCTCTGTTCTGAACT+TGG - chr2.2:68775890-68775909 MS.gene01449:CDS 45.0%
! GAGGATCTCACAAGAGGTTT+TGG - chr2.2:68774716-68774735 MS.gene01449:intron 45.0%
! GGTTTCGGTTTGAATCTCTC+CGG - chr2.2:68775103-68775122 MS.gene01449:intron 45.0%
! GTGGAGCAAGACTGTACTTT+TGG - chr2.2:68776418-68776437 MS.gene01449:intron 45.0%
! TATTCGTTTCCTGGTTCGCT+GGG - chr2.2:68775256-68775275 MS.gene01449:intron 45.0%
! TGCTGCCTCTGATTTTGCTT+TGG + chr2.2:68777265-68777284 None:intergenic 45.0%
! TTATTCGTTTCCTGGTTCGC+TGG - chr2.2:68775255-68775274 MS.gene01449:intron 45.0%
!! CCCATTTGTGTTGGTGATGA+TGG - chr2.2:68775052-68775071 MS.gene01449:intron 45.0%
!! CGGATTTTGGAAGCTCTCTT+AGG + chr2.2:68775984-68776003 None:intergenic 45.0%
!!! GAGGTTTTGGGTGATGCTTT+AGG - chr2.2:68774729-68774748 MS.gene01449:intron 45.0%
AAGCTTCTTCTGGCAACCAG+TGG + chr2.2:68774555-68774574 None:intergenic 50.0%
AGTGCTCCTCCTTGAGGTTT+CGG - chr2.2:68775088-68775107 MS.gene01449:intron 50.0%
CCTCTAGATTGAAGGAGCAG+AGG - chr2.2:68777304-68777323 MS.gene01449:intron 50.0%
CCTCTGCTCCTTCAATCTAG+AGG + chr2.2:68777307-68777326 None:intergenic 50.0%
CGAAGAAGGAAGTCTGTTCG+TGG - chr2.2:68775852-68775871 MS.gene01449:CDS 50.0%
CGGACTTTCACAACCAAAGC+AGG - chr2.2:68776062-68776081 MS.gene01449:intron 50.0%
CTCTTAGGACCCCTCATTCT+TGG + chr2.2:68775969-68775988 None:intergenic 50.0%
GAAGAGTCAAAGGAGTGACC+AGG + chr2.2:68777200-68777219 None:intergenic 50.0%
GGACAAGAGGATCTCACAAG+AGG - chr2.2:68774710-68774729 MS.gene01449:intron 50.0%
GGGTTCCTTTCCTGCTTTCA+TGG - chr2.2:68777006-68777025 MS.gene01449:intron 50.0%
TTCTTCTGGCAACCAGTGGT+TGG + chr2.2:68774551-68774570 None:intergenic 50.0%
! ACTCCTTGTTTCCGTGGACA+CGG - chr2.2:68775813-68775832 MS.gene01449:CDS 50.0%
! TGACCAGGCGCTGTATCTTT+GGG + chr2.2:68777185-68777204 None:intergenic 50.0%
! TGCGTGATTTTGCTACTCGC+AGG - chr2.2:68774824-68774843 MS.gene01449:intron 50.0%
!! TGGTGATGATGGCTTGACCT+TGG - chr2.2:68775063-68775082 MS.gene01449:intron 50.0%
!!! GTCGTGCATTTTGGGACAAG+AGG - chr2.2:68774697-68774716 MS.gene01449:intron 50.0%
AACACGTCAAAGGAGGGGAG+TGG + chr2.2:68775676-68775695 None:intergenic 55.0%
AAGCAGGGTGTGTTAACCCC+TGG - chr2.2:68774906-68774925 MS.gene01449:intron 55.0%
AATACCAGCGAGAATGGGGC+TGG - chr2.2:68775618-68775637 MS.gene01449:intron 55.0%
CGCTGCAACACCTTGCTTAG+CGG + chr2.2:68777376-68777395 None:intergenic 55.0%
CTTCCGTGTCCACGGAAACA+AGG + chr2.2:68775819-68775838 None:intergenic 55.0%
CTTGTTTCCGTGGACACGGA+AGG - chr2.2:68775817-68775836 MS.gene01449:CDS 55.0%
GGTTGTGAAAGTCCGACGGT+AGG + chr2.2:68776057-68776076 None:intergenic 55.0%
TGCAACACCTTGCTTAGCGG+TGG + chr2.2:68777373-68777392 None:intergenic 55.0%
TTAGAGACGGCAAAGTGGCC+AGG + chr2.2:68775708-68775727 None:intergenic 55.0%
! GACCAGGCGCTGTATCTTTG+GGG + chr2.2:68777184-68777203 None:intergenic 55.0%
! GCAGGTACTCCTTGTTTCCG+TGG - chr2.2:68775807-68775826 MS.gene01449:CDS 55.0%
! GGCCACTTTGCCGTCTCTAA+TGG - chr2.2:68775708-68775727 MS.gene01449:intron 55.0%
! GTGACCAGGCGCTGTATCTT+TGG + chr2.2:68777186-68777205 None:intergenic 55.0%
!! TCAAGGAGGAGCACTCTCCA+AGG + chr2.2:68775083-68775102 None:intergenic 55.0%
!! TTGGAGAGTGCTCCTCCTTG+AGG - chr2.2:68775082-68775101 MS.gene01449:intron 55.0%
AGCAAGAGGCGAACACGACC+AGG + chr2.2:68774927-68774946 None:intergenic 60.0%
AGCCCCAAAGATACAGCGCC+TGG - chr2.2:68777179-68777198 MS.gene01449:intron 60.0%
AGGCTCTCCACCGCTAAGCA+AGG - chr2.2:68777363-68777382 MS.gene01449:intron 60.0%
CAAGAGGCGAACACGACCAG+GGG + chr2.2:68774925-68774944 None:intergenic 60.0%
CATTGCGCCTTCCGTGTCCA+CGG + chr2.2:68775827-68775846 None:intergenic 60.0%
GCAAGAGGCGAACACGACCA+GGG + chr2.2:68774926-68774945 None:intergenic 60.0%
GGAACGCCGCAGTGAGAGTT+TGG - chr2.2:68777326-68777345 MS.gene01449:intron 60.0%
GGCGCAATGGAGAGCGAAGA+AGG - chr2.2:68775838-68775857 MS.gene01449:CDS 60.0%
GTCAAAGGAGGGGAGTGGCA+GGG + chr2.2:68775671-68775690 None:intergenic 60.0%
GTTCGCCTCTTGCTCCACAG+AGG - chr2.2:68774933-68774952 MS.gene01449:intron 60.0%
AGTGGCAGGGCAACGTGAGG+CGG + chr2.2:68775658-68775677 None:intergenic 65.0%
CGTCAAAGGAGGGGAGTGGC+AGG + chr2.2:68775672-68775691 None:intergenic 65.0%
GTCACCAGCCCCATTCTCGC+TGG + chr2.2:68775625-68775644 None:intergenic 65.0%
! CGTGGACACGGAAGGCGCAA+TGG - chr2.2:68775825-68775844 MS.gene01449:CDS 65.0%
! TCCTGGTTCGCTGGGCTGTC+CGG - chr2.2:68775264-68775283 MS.gene01449:intron 65.0%
ACCGGACAGCCCAGCGAACC+AGG + chr2.2:68775268-68775287 None:intergenic 70.0%
GGGAGTGGCAGGGCAACGTG+AGG + chr2.2:68775661-68775680 None:intergenic 70.0%
GTGGCAGGGCAACGTGAGGC+GGG + chr2.2:68775657-68775676 None:intergenic 70.0%
TGGCAGGGCAACGTGAGGCG+GGG + chr2.2:68775656-68775675 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 68774445 68777398 68774445 ID=MS.gene01449
chr2.2 mRNA 68774445 68777398 68774445 ID=MS.gene01449.t1;Parent=MS.gene01449
chr2.2 exon 68777393 68777398 68777393 ID=MS.gene01449.t1.exon1;Parent=MS.gene01449.t1
chr2.2 CDS 68777393 68777398 68777393 ID=cds.MS.gene01449.t1;Parent=MS.gene01449.t1
chr2.2 exon 68777246 68777310 68777246 ID=MS.gene01449.t1.exon2;Parent=MS.gene01449.t1
chr2.2 CDS 68777246 68777310 68777246 ID=cds.MS.gene01449.t1;Parent=MS.gene01449.t1
chr2.2 exon 68777088 68777145 68777088 ID=MS.gene01449.t1.exon3;Parent=MS.gene01449.t1
chr2.2 CDS 68777088 68777145 68777088 ID=cds.MS.gene01449.t1;Parent=MS.gene01449.t1
chr2.2 exon 68776889 68776997 68776889 ID=MS.gene01449.t1.exon4;Parent=MS.gene01449.t1
chr2.2 CDS 68776889 68776997 68776889 ID=cds.MS.gene01449.t1;Parent=MS.gene01449.t1
chr2.2 exon 68775760 68776032 68775760 ID=MS.gene01449.t1.exon5;Parent=MS.gene01449.t1
chr2.2 CDS 68775760 68776032 68775760 ID=cds.MS.gene01449.t1;Parent=MS.gene01449.t1
chr2.2 exon 68774445 68774680 68774445 ID=MS.gene01449.t1.exon6;Parent=MS.gene01449.t1
chr2.2 CDS 68774445 68774680 68774445 ID=cds.MS.gene01449.t1;Parent=MS.gene01449.t1
Gene Sequence

>MS.gene01449

ATGAAGGTTACTCCCCCAAACCCTAATTTCCCTTGTTCCTTTGATTTCATTGCTCTTATCACTAAACCATATAGAAATTGTTTTGCAGTTCAACGTTGCAAATCCAACCACTGGTTGCCAGAAGAAGCTTGAAATCGATGACGACATGAAGCTGTAATCTCTTCACCTTTTTTTTTTTTTTTTCTTTGCGTTTCTTCTATTCACACGATTTGTTCTCATGTTCATAATTGTTTTTTTTTGTTGTAAATTGTAGTCGTGCATTTTGGGACAAGAGGATCTCACAAGAGGTTTTGGGTGATGCTTTAGGCGAAGTATGTTTGAATCTAGAAAAAATATTTGAATAATCCTTAAATTAATTGTTATATTGATTTATAACTGTTGCGTGATTTTGCTACTCGCAGGAATTTAAGGGATATGTCTTCAAAATCACTGGTGGATGTGACAAACAAGGATTTCCAATGAAGCAGGGTGTGTTAACCCCTGGTCGTGTTCGCCTCTTGCTCCACAGAGGTATAACCTTTCTACAGTTTTTTTTTTTTTTTTTTGTGTTTTTATTAATTATTATAGTGAGGTTAATGATTATACTTAAAATTATATGTACTATAAACCCATTTGTGTTGGTGATGATGGCTTGACCTTGGAGAGTGCTCCTCCTTGAGGTTTCGGTTTGAATCTCTCCGGTTCCAATTTAGATGGGCTAATTTAGCTTTTTCAAAAGAAAAATTATATGTACTATTATTTCCATTATAGGTGTTGTATACTCTTTATCATTTACTTGAATGGTTCCTTTTGTTTTTATTACTGTTTGAATTATTCGTTTCCTGGTTCGCTGGGCTGTCCGGTTTAACCCTCTATTTTAGATCTTTTTTTCACTAATAGGAGACCCAAACTGGTGAATTTATTTTGTATGTTAGTGGATTTTTTGTAGGCATTTCATGTTTGCTTGTTGTTGTTGCTGTTGTTAATTATTCCTTTTTAGTTTTGTTAATTATGCTGTTTGATTGTTCATGATGTTTGATTTATTAATTATGTTGTTCGGGTGGCATTGAGCCATTTATGTTTTTCCCCCAAATTTGTCAGCTGCCAATTTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATGCAGCAATATGTTTTTTATAATACCAGCGAGAATGGGGCTGGTGACTATTTGAGCCCCGCCTCACGTTGCCCTGCCACTCCCCTCCTTTGACGTGTTAATTGCTGACCTGGCCACTTTGCCGTCTCTAATGGTAGATGGTTCTCTTAGCAAAGTAGTAAGCCCTTGTTAAGTATTTATTTGGTAAGTTTTAATTTAATGTTTGTTTGAGCAGGTACTCCTTGTTTCCGTGGACACGGAAGGCGCAATGGAGAGCGAAGAAGGAAGTCTGTTCGTGGGTGCATTGTCAGCCCAGACCTCTCTGTTCTGAACTTGGTTATTGTGAAGAAGGGTGAAAATGATTTGCCTGGTTTGACTGATGTTGAAAAGCCAAGAATGAGGGGTCCTAAGAGAGCTTCCAAAATCCGTAAGCTCTTCAATCTTTCCAAGGATGATGATGTCAGGAAGTATGTCAACACCTACCGTCGGACTTTCACAACCAAAGCAGGTAAAATTTCTTCCTGATTCTCTGTTGATAATACATAGCTTTGGTTTTGAAAAATGGTCTTGGAGAACATCCTATTTAGTATTTATCACTTGAAATATATTGTCTCCAATATCTAAGTTGGAATTTTTCAAAATCACTTGCTATTATAATATAGGGTGAGTCTGGTTGGTGAAACTTTACTGTTAAACTTTAAACTTGCTGTATGTATTGTTAGTTATAGGGTGAGTCTGGTTGGTGAAACTTTACTGTTAAACTTTAAACTTGCTGTATGTATTGTTAGTTAACATCATATAAGAAATTATGCAATTCAGTTGCCCATTGTATAGATGAGTTTGTGGAGCAAGACTGTACTTTTGGAATAGTGTGTTTCCCTTCCCATCAATCCTTGAAAAATTGAAAGAAGCTAATACACACTGCTTAAGAAGCCGACTTCTATCCCTTCCCACTAAACTTAAAACCTTTGTTTTGTATATTAATTATGCTGTGTTATGGTTAGAAGTATAGGATGATAGAACAAAGTGACTGCTGTTATAAATGTCGTTAGTTAGTTAGTTAACATTGGGAAGGTAGTTGGAAAGTAGTTTAGTAATTGCGGAGTGAATGCAAGAGAATATTTAGTTGTTTTGAGAGGAAGATTTCAGGTTTCTCTAATAACCTGATAGATTGCTAAATAGATGAAGAGGGATATTCCCGTTGCTGTTAATAGGAACTAGATTAAAAAATAGACACAAGAACTGAATTGCAACAGGGATATCCCTCTGATTCTATTTTTAAATCTCCCAAAACAATTGCAAATTGACAAAACTGACTTCTCTTGCATACAATCTGCTATTGATAGACCACTCTCAACTAATTTCGTACTGCCAGCTAACTTACGACAATTATTACAGCGGTCGGTTGCAAAATATCCGCTCTTTTATTTGGGTTCCTTTCCTGCTTTCATGGACTTGCATGATAGGAGGTTGACTTGTTTTATCAGAGGACTCATTTTGTAATAGAATATTATCTATGGTCATAACTATGGATCTATAATTTATAATGGTTTCTAGTCACAAAGTAACACGTATCTGATTGTGCAGGGAAAAAGCAGAGCAAAGCCCCAAAGATACAGCGCCTGGTCACTCCTTTGACTCTTCAAAGAAAGAGGGCAAGAATTGCTGATAAGAAGAAAAGAATTGCCAAAGCAAAATCAGAGGCAGCAGATTACCAAAAACTTCTTGCCTCTAGATTGAAGGAGCAGAGGGAACGCCGCAGTGAGAGTTTGGCTAAGAAGAGATCAAGGCTCTCCACCGCTAAGCAAGGTGTTGCAGCGTAG
Protein sequence

>MS.gene01449.t1

MKFNVANPTTGCQKKLEIDDDMKLRAFWDKRISQEVLGDALGEEFKGYVFKITGGCDKQGFPMKQGVLTPGRVRLLLHRGTPCFRGHGRRNGERRRKSVRGCIVSPDLSVLNLVIVKKGENDLPGLTDVEKPRMRGPKRASKIRKLFNLSKDDDVRKYVNTYRRTFTTKAGKKQSKAPKIQRLVTPLTLQRKRARIADKKKRIAKAKSEAADYQKLLASRLKEQRERRSESLAKKRSRLSTAKQGVAA