AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene39485

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39485.t1 MTR_4g088595 100.000 248 0 0 1 248 1 248 0.0 501
MS.gene39485.t1 MTR_7g053160 96.774 248 8 0 1 248 1 248 4.65e-177 486
MS.gene39485.t1 MTR_2g014030 96.774 248 8 0 1 248 1 248 2.02e-176 485
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39485.t1 AT5G10360 91.968 249 18 1 1 247 1 249 7.41e-165 456
MS.gene39485.t1 AT4G31700 91.600 250 19 1 1 248 1 250 1.33e-157 437
MS.gene39485.t1 AT5G10360 73.896 249 11 2 1 247 1 197 4.09e-120 340
MS.gene39485.t1 AT4G31700 90.957 188 15 1 63 248 1 188 2.59e-109 313

Find 78 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATATTGCAGCCGTGCTTTC+TGG 0.027256 4.2:+22514482 MS.gene39485:intron
TTGCTCTCTGCAGGAGTTTA+AGG 0.158668 4.2:+22514632 MS.gene39485:intron
GTTGAACAGCTTACGGATTT+TGG 0.198177 4.2:-22515483 None:intergenic
CAATTCTTGCTCGCTTCCTT+TGG 0.278485 4.2:-22516018 None:intergenic
GTGACCAATCGCTGTATCTT+TGG 0.338640 4.2:-22515983 None:intergenic
GGAAGTTCTTGGTGATGCTT+TGG 0.339745 4.2:+22514522 MS.gene39485:CDS
ATATTGCAGCCGTGCTTTCT+GGG 0.350681 4.2:+22514483 MS.gene39485:intron
TTCCCTCTGCTCCTTCAATC+TGG 0.366847 4.2:-22516107 None:intergenic
CTCTTAGGACCTCTCATCCT+TGG 0.367925 4.2:-22515455 None:intergenic
AAAGGTGATAATGATCTTCC+AGG 0.375447 4.2:+22515413 MS.gene39485:CDS
GTCAACACTCCCTGCTTCAT+TGG 0.384872 4.2:-22514699 None:intergenic
TGTTGACCCCTGGCCGTGTT+AGG 0.390290 4.2:+22514715 MS.gene39485:CDS
GGCGCAATGGAGAGCGTAGA+AGG 0.390771 4.2:+22515327 MS.gene39485:CDS
TGCTCTCTGCAGGAGTTTAA+GGG 0.392051 4.2:+22514633 MS.gene39485:intron
ACTCCTTGCTTCCAGATTGA+AGG 0.399330 4.2:+22516096 MS.gene39485:CDS
AGGATCTCACAGGAAGTTCT+TGG 0.420484 4.2:+22514511 MS.gene39485:CDS
GAAGTTCTTGGTGATGCTTT+GGG 0.420593 4.2:+22514523 MS.gene39485:CDS
TGGCTTAGCACTGGATAGTC+TGG 0.428627 4.2:-22516161 None:intergenic
CGCTTCCTTTGGAGTGTCAA+AGG 0.447165 4.2:-22516007 None:intergenic
TCAACACTCCCTGCTTCATT+GGG 0.455023 4.2:-22514698 None:intergenic
CTGACATCCAGTGGTTGGAT+TGG 0.459762 4.2:-22513859 None:intergenic
TTGTTTGATTGCAGGGAAGA+AGG 0.460053 4.2:+22515949 MS.gene39485:intron
TTGACAGGTTGAACAGCTTA+CGG 0.462619 4.2:-22515490 None:intergenic
AGCAAGAGCCTAACACGGCC+AGG 0.463109 4.2:-22514723 None:intergenic
ACAAGGATTCCCAATGAAGC+AGG 0.478682 4.2:+22514689 MS.gene39485:CDS
TTCTTCTGACATCCAGTGGT+TGG 0.481727 4.2:-22513864 None:intergenic
AAGTTCAAAACAGAGAGGTC+GGG 0.481755 4.2:-22515377 None:intergenic
GCTGCTTATGCTGTGGCAGA+TGG 0.483327 4.2:-22516181 None:intergenic
GCACATCATCATCCTTTGAC+AGG 0.490483 4.2:-22515505 None:intergenic
AACTTGGTTATTGTGAAGAA+AGG 0.498754 4.2:+22515395 MS.gene39485:CDS
AATCAGAAACTTACCATCCT+TGG 0.507683 4.2:-22515564 None:intergenic
AACGTTGCCAATCCAACCAC+TGG 0.507932 4.2:+22513852 MS.gene39485:CDS
GCAAGAGCCTAACACGGCCA+GGG 0.526221 4.2:-22514722 None:intergenic
GGTTATGTCTTCAAGATCAC+TGG 0.529877 4.2:+22514654 MS.gene39485:CDS
AAGCTTCTTCTGACATCCAG+TGG 0.530433 4.2:-22513868 None:intergenic
TATGTCTTCAAGATCACTGG+TGG 0.541337 4.2:+22514657 MS.gene39485:CDS
AAGCAGGGAGTGTTGACCCC+TGG 0.544754 4.2:+22514705 MS.gene39485:CDS
CTTCCGTGTCCACGGAAACA+AGG 0.545510 4.2:-22515305 None:intergenic
TTCTCAACATCAGTAAGCCC+TGG 0.548857 4.2:-22515431 None:intergenic
GCCGTGCTTTCTGGGACAAG+AGG 0.553103 4.2:+22514491 MS.gene39485:intron
CAAGTTCAAAACAGAGAGGT+CGG 0.553854 4.2:-22515378 None:intergenic
GGAACGCCGAAGTGAGAGCT+TGG 0.554466 4.2:+22516126 MS.gene39485:CDS
CTTGTTTCCGTGGACACGGA+AGG 0.556226 4.2:+22515306 MS.gene39485:CDS
GCAGGAACTCCTTGTTTCCG+TGG 0.557291 4.2:+22515296 MS.gene39485:intron
CAGAAACTTACCATCCTTGG+TGG 0.557776 4.2:-22515561 None:intergenic
CAAGAAGAAGAGAATTGCTA+AGG 0.559648 4.2:+22516045 MS.gene39485:CDS
AGCTCCAAAGATACAGCGAT+TGG 0.560639 4.2:+22515979 MS.gene39485:CDS
TCCTCTTGTCCCAGAAAGCA+CGG 0.564472 4.2:-22514492 None:intergenic
CGTGGACACGGAAGGCGCAA+TGG 0.565614 4.2:+22515314 MS.gene39485:CDS
ACTCCTTGTTTCCGTGGACA+CGG 0.569669 4.2:+22515302 MS.gene39485:CDS
CGTAGAAGGAAGTCTGTTCG+TGG 0.573391 4.2:+22515341 MS.gene39485:CDS
GCTCCTTCAATCTGGAAGCA+AGG 0.577913 4.2:-22516099 None:intergenic
CCTTGGTGGTGAAAGTTCTA+CGG 0.579080 4.2:-22515547 None:intergenic
TTCCAGATTGAAGGAGCAGA+GGG 0.584168 4.2:+22516105 MS.gene39485:CDS
AGAACTTTCACCACCAAGGA+TGG 0.589936 4.2:+22515551 MS.gene39485:CDS
CATTGCGCCTTCCGTGTCCA+CGG 0.591891 4.2:-22515313 None:intergenic
CTGGGACAAGAGGATCTCAC+AGG 0.592723 4.2:+22514501 MS.gene39485:CDS
TAAGCTGTTCAACCTGTCAA+AGG 0.594543 4.2:+22515493 MS.gene39485:CDS
AGTTCAAAACAGAGAGGTCG+GGG 0.595017 4.2:-22515376 None:intergenic
TTACTGATGTTGAGAAGCCA+AGG 0.596042 4.2:+22515438 MS.gene39485:CDS
GTTGAGAAGCCAAGGATGAG+AGG 0.601224 4.2:+22515446 MS.gene39485:CDS
TGTGGCAGATGGCTTAGCAC+TGG 0.603451 4.2:-22516170 None:intergenic
AAGGTGATAATGATCTTCCA+GGG 0.607328 4.2:+22515414 MS.gene39485:CDS
GTTAGGCTCTTGCTCCATAG+AGG 0.608724 4.2:+22514732 MS.gene39485:CDS
TCACTCCTTTGACACTCCAA+AGG 0.610174 4.2:+22516002 MS.gene39485:CDS
TCTTAGCCAAGCTCTCACTT+CGG 0.610174 4.2:-22516132 None:intergenic
GTGAGAGCTTGGCTAAGAAG+AGG 0.611225 4.2:+22516137 MS.gene39485:CDS
CAAGAGCCTAACACGGCCAG+GGG 0.618346 4.2:-22514721 None:intergenic
CTTCCAGATTGAAGGAGCAG+AGG 0.620391 4.2:+22516104 MS.gene39485:CDS
ATGTCTTCAAGATCACTGGT+GGG 0.622519 4.2:+22514658 MS.gene39485:CDS
AATTGCTAAGGCAAAATCAG+AGG 0.627856 4.2:+22516057 MS.gene39485:CDS
GGTGGTGAAAGTTCTACGGT+AGG 0.627958 4.2:-22515543 None:intergenic
CAAGGATTCCCAATGAAGCA+GGG 0.635106 4.2:+22514690 MS.gene39485:CDS
ACTGGTGGGTGTGACAAACA+AGG 0.636404 4.2:+22514672 MS.gene39485:CDS
GCTGTAAGCTGCTTATGCTG+TGG 0.655143 4.2:-22516188 None:intergenic
CCGTAGAACTTTCACCACCA+AGG 0.691015 4.2:+22515547 MS.gene39485:CDS
TATGGAGCAAGAGCCTAACA+CGG 0.710814 4.2:-22514728 None:intergenic
TAACCAAGTTCAAAACAGAG+AGG 0.738467 4.2:-22515382 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATATATCCTTATATTAA+GGG + chr4.2:22515609-22515628 MS.gene39485:intron 10.0%
!! CTTTATATATCCTTATATTA+AGG + chr4.2:22515608-22515627 MS.gene39485:intron 15.0%
!! TATAAGGATATATAAAGAAA+TGG - chr4.2:22515605-22515624 None:intergenic 15.0%
!! AAGAATATTACTTTCATTGA+AGG + chr4.2:22515770-22515789 MS.gene39485:intron 20.0%
!! ACATGAGATAAATAGTAAAA+TGG - chr4.2:22514900-22514919 None:intergenic 20.0%
!! CAGAAAATTGAAAAGAATTT+CGG - chr4.2:22514046-22514065 None:intergenic 20.0%
!! TAGCTTATGAAAATAGTTTA+TGG + chr4.2:22514300-22514319 MS.gene39485:intron 20.0%
!! TTCATTACAAATATCTGTAT+TGG - chr4.2:22515911-22515930 None:intergenic 20.0%
!!! TCATTTGAAGTATTTTTCTT+AGG + chr4.2:22513952-22513971 MS.gene39485:intron 20.0%
!!! TTAAGTTGTTTATCAAAACA+AGG + chr4.2:22514433-22514452 MS.gene39485:intron 20.0%
! AAAAAAAAATGCTACCTCTA+TGG - chr4.2:22514749-22514768 None:intergenic 25.0%
! AACAATAATCATTGTGATCA+TGG - chr4.2:22515166-22515185 None:intergenic 25.0%
! AAGTTATCTCTAATATTACG+TGG - chr4.2:22514982-22515001 None:intergenic 25.0%
! ACATCCAAAACTATTTAACT+AGG - chr4.2:22515060-22515079 None:intergenic 25.0%
! AGAACAAAAATGAATACAAG+TGG - chr4.2:22515712-22515731 None:intergenic 25.0%
! CGTTCATAAATTTATAGTAG+TGG + chr4.2:22514851-22514870 MS.gene39485:intron 25.0%
! CTTAGTATTTCATTATTTGC+AGG + chr4.2:22515225-22515244 MS.gene39485:intron 25.0%
! GCAAATAATGAAATACTAAG+TGG - chr4.2:22515225-22515244 None:intergenic 25.0%
! GTAATGAATTGTAATCTTAG+TGG + chr4.2:22515923-22515942 MS.gene39485:intron 25.0%
! TCATAAATTTATAGTAGTGG+AGG + chr4.2:22514854-22514873 MS.gene39485:intron 25.0%
! TCTTCAATCTATTTACCTTT+CGG + chr4.2:22514777-22514796 MS.gene39485:intron 25.0%
! TTACAATGCTCTACAAAATA+AGG - chr4.2:22514589-22514608 None:intergenic 25.0%
!! ACTATTTTCATAAGCTATCT+TGG - chr4.2:22514297-22514316 None:intergenic 25.0%
!! AGTTCTTTTCATAAAGTTAG+TGG - chr4.2:22515028-22515047 None:intergenic 25.0%
!! CATTGTCATTTATCTGAATT+TGG + chr4.2:22514160-22514179 MS.gene39485:intron 25.0%
!! TCAATATGTTTTGTAATGCA+TGG + chr4.2:22514128-22514147 MS.gene39485:intron 25.0%
!!! ATTTGGTTTGAACTGATATT+TGG + chr4.2:22514177-22514196 MS.gene39485:intron 25.0%
!!! TTAAATAGTTTTGGATGTGT+GGG + chr4.2:22515062-22515081 MS.gene39485:intron 25.0%
!!! TTTATTTTATCGTGTGTAGT+AGG + chr4.2:22514345-22514364 MS.gene39485:intron 25.0%
AACTTGGTTATTGTGAAGAA+AGG + chr4.2:22515395-22515414 MS.gene39485:CDS 30.0%
ACAAAAATGAATACAAGTGG+AGG - chr4.2:22515709-22515728 None:intergenic 30.0%
ACAGTATACATTGTCTAGAT+TGG + chr4.2:22515805-22515824 MS.gene39485:intron 30.0%
AGTATACATTGTCTAGATTG+GGG + chr4.2:22515807-22515826 MS.gene39485:intron 30.0%
ATATGACTGTAGTGTAAATC+TGG + chr4.2:22514096-22514115 MS.gene39485:intron 30.0%
ATCCTTATATTAAGGGTAGT+GGG + chr4.2:22515616-22515635 MS.gene39485:intron 30.0%
CAGTATACATTGTCTAGATT+GGG + chr4.2:22515806-22515825 MS.gene39485:intron 30.0%
CCAAAACTATTTAACTAGGA+GGG - chr4.2:22515056-22515075 None:intergenic 30.0%
CCAACATAGATTTATGAACA+TGG - chr4.2:22514002-22514021 None:intergenic 30.0%
CCATGTTCATAAATCTATGT+TGG + chr4.2:22513999-22514018 MS.gene39485:intron 30.0%
GAAAATGAAGAGAGAGTAAA+TGG - chr4.2:22513925-22513944 None:intergenic 30.0%
GTGAACTTGTAGAAATAAGT+TGG - chr4.2:22514275-22514294 None:intergenic 30.0%
TATCCTTATATTAAGGGTAG+TGG + chr4.2:22515615-22515634 MS.gene39485:intron 30.0%
TCCAAAACTATTTAACTAGG+AGG - chr4.2:22515057-22515076 None:intergenic 30.0%
TCTCTGATAGTAATACTAAG+AGG - chr4.2:22514831-22514850 None:intergenic 30.0%
! GCATATGTAGTTATTTCAGT+AGG + chr4.2:22514948-22514967 MS.gene39485:intron 30.0%
! GTGATTTTGCATTGGATTAT+TGG + chr4.2:22514918-22514937 MS.gene39485:intron 30.0%
! TATCTCATGTGATTTTGCAT+TGG + chr4.2:22514910-22514929 MS.gene39485:intron 30.0%
! TGTAGAAATAAGTTGGAAAC+AGG - chr4.2:22514268-22514287 None:intergenic 30.0%
!! CTGTATTGGTACAATTTTAC+CGG - chr4.2:22515897-22515916 None:intergenic 30.0%
!!! GTTAAATAGTTTTGGATGTG+TGG + chr4.2:22515061-22515080 MS.gene39485:intron 30.0%
AAAAAATCCATGCCATAGTG+AGG + chr4.2:22515106-22515125 MS.gene39485:intron 35.0%
AAAAATCCATGCCATAGTGA+GGG + chr4.2:22515107-22515126 MS.gene39485:intron 35.0%
AAACAGTTGTCCAAACTACT+AGG + chr4.2:22515673-22515692 MS.gene39485:intron 35.0%
AACAGTTGTCCAAACTACTA+GGG + chr4.2:22515674-22515693 MS.gene39485:intron 35.0%
AATCAGAAACTTACCATCCT+TGG - chr4.2:22515567-22515586 None:intergenic 35.0%
AATCGCAAATTGTGAGGAAA+CGG - chr4.2:22515859-22515878 None:intergenic 35.0%
AATTGCTAAGGCAAAATCAG+AGG + chr4.2:22516057-22516076 MS.gene39485:CDS 35.0%
AGAAATGGTTACGATATTCG+TGG - chr4.2:22515590-22515609 None:intergenic 35.0%
CAAGAAGAAGAGAATTGCTA+AGG + chr4.2:22516045-22516064 MS.gene39485:CDS 35.0%
CTAAACAAACCAATCCAAAC+AGG - chr4.2:22514205-22514224 None:intergenic 35.0%
TAACCAAGTTCAAAACAGAG+AGG - chr4.2:22515385-22515404 None:intergenic 35.0%
TCCTTATATTAAGGGTAGTG+GGG + chr4.2:22515617-22515636 MS.gene39485:intron 35.0%
TGGGAAACATGCATATAGTA+TGG + chr4.2:22515081-22515100 MS.gene39485:intron 35.0%
! AAAGGTGATAATGATCTTCC+AGG + chr4.2:22515413-22515432 MS.gene39485:CDS 35.0%
! AAGGTGATAATGATCTTCCA+GGG + chr4.2:22515414-22515433 MS.gene39485:CDS 35.0%
! CCCTCCTAGTTAAATAGTTT+TGG + chr4.2:22515053-22515072 MS.gene39485:intron 35.0%
! GAGTAAGCTTCTTTTCTACT+TGG + chr4.2:22515247-22515266 MS.gene39485:intron 35.0%
! TTATGCATTTTGCACTTTGC+AGG + chr4.2:22515278-22515297 MS.gene39485:intron 35.0%
AAACGGCACTTCTTTCAAAC+GGG - chr4.2:22515842-22515861 None:intergenic 40.0%
AAGGACAATCGCAAATTGTG+AGG - chr4.2:22515865-22515884 None:intergenic 40.0%
AAGTTCAAAACAGAGAGGTC+GGG - chr4.2:22515380-22515399 None:intergenic 40.0%
ACAGTTGTCCAAACTACTAG+GGG + chr4.2:22515675-22515694 MS.gene39485:intron 40.0%
ATGTCTTCAAGATCACTGGT+GGG + chr4.2:22514658-22514677 MS.gene39485:CDS 40.0%
CAAGTTCAAAACAGAGAGGT+CGG - chr4.2:22515381-22515400 None:intergenic 40.0%
GAAACGGCACTTCTTTCAAA+CGG - chr4.2:22515843-22515862 None:intergenic 40.0%
GGTTATGTCTTCAAGATCAC+TGG + chr4.2:22514654-22514673 MS.gene39485:CDS 40.0%
TAAAGTTAGTGGAGACGTTG+AGG - chr4.2:22515017-22515036 None:intergenic 40.0%
TAAGCTGTTCAACCTGTCAA+AGG + chr4.2:22515493-22515512 MS.gene39485:CDS 40.0%
TATGTCTTCAAGATCACTGG+TGG + chr4.2:22514657-22514676 MS.gene39485:CDS 40.0%
TTACTGATGTTGAGAAGCCA+AGG + chr4.2:22515438-22515457 MS.gene39485:CDS 40.0%
TTGTTTGATTGCAGGGAAGA+AGG + chr4.2:22515949-22515968 MS.gene39485:intron 40.0%
! ACTGATATTTGGAGCCTGTT+TGG + chr4.2:22514188-22514207 MS.gene39485:intron 40.0%
! ATTTTACCGGATGTCACTGA+AGG - chr4.2:22515884-22515903 None:intergenic 40.0%
! CCTTATATTAAGGGTAGTGG+GGG + chr4.2:22515618-22515637 MS.gene39485:intron 40.0%
! GTTGAACAGCTTACGGATTT+TGG - chr4.2:22515486-22515505 None:intergenic 40.0%
! TATTTGGAGCCTGTTTGGAT+TGG + chr4.2:22514193-22514212 MS.gene39485:intron 40.0%
! TTGACAGGTTGAACAGCTTA+CGG - chr4.2:22515493-22515512 None:intergenic 40.0%
!! AGTGGTTGTTGTTTGATTGC+AGG + chr4.2:22515941-22515960 MS.gene39485:intron 40.0%
!! GAAGTTCTTGGTGATGCTTT+GGG + chr4.2:22514523-22514542 MS.gene39485:CDS 40.0%
!! GTGGTTGTTGTTTGATTGCA+GGG + chr4.2:22515942-22515961 MS.gene39485:intron 40.0%
AAGCTTCTTCTGACATCCAG+TGG - chr4.2:22513871-22513890 None:intergenic 45.0%
ACAAGGATTCCCAATGAAGC+AGG + chr4.2:22514689-22514708 MS.gene39485:CDS 45.0%
ACTCCTTGCTTCCAGATTGA+AGG + chr4.2:22516096-22516115 MS.gene39485:CDS 45.0%
AGAACTTTCACCACCAAGGA+TGG + chr4.2:22515551-22515570 MS.gene39485:CDS 45.0%
AGCTCCAAAGATACAGCGAT+TGG + chr4.2:22515979-22515998 MS.gene39485:CDS 45.0%
AGGATCTCACAGGAAGTTCT+TGG + chr4.2:22514511-22514530 MS.gene39485:CDS 45.0%
AGTTCAAAACAGAGAGGTCG+GGG - chr4.2:22515379-22515398 None:intergenic 45.0%
ATATTGCAGCCGTGCTTTCT+GGG + chr4.2:22514483-22514502 MS.gene39485:intron 45.0%
CAAGGATTCCCAATGAAGCA+GGG + chr4.2:22514690-22514709 MS.gene39485:CDS 45.0%
CAATTCTTGCTCGCTTCCTT+TGG - chr4.2:22516021-22516040 None:intergenic 45.0%
CAGAAACTTACCATCCTTGG+TGG - chr4.2:22515564-22515583 None:intergenic 45.0%
CCCCCACTACCCTTAATATA+AGG - chr4.2:22515621-22515640 None:intergenic 45.0%
CTATGAAGCCCCTAGTAGTT+TGG - chr4.2:22515686-22515705 None:intergenic 45.0%
GATTGTCCTTCAGTGACATC+CGG + chr4.2:22515875-22515894 MS.gene39485:intron 45.0%
GCAACATACAGTATGCCGAA+AGG - chr4.2:22514795-22514814 None:intergenic 45.0%
GCACATCATCATCCTTTGAC+AGG - chr4.2:22515508-22515527 None:intergenic 45.0%
GTGACCAATCGCTGTATCTT+TGG - chr4.2:22515986-22516005 None:intergenic 45.0%
TATATTGCAGCCGTGCTTTC+TGG + chr4.2:22514482-22514501 MS.gene39485:intron 45.0%
TATGGAGCAAGAGCCTAACA+CGG - chr4.2:22514731-22514750 None:intergenic 45.0%
TCAACACTCCCTGCTTCATT+GGG - chr4.2:22514701-22514720 None:intergenic 45.0%
TCACTCCTTTGACACTCCAA+AGG + chr4.2:22516002-22516021 MS.gene39485:CDS 45.0%
TCTTAGCCAAGCTCTCACTT+CGG - chr4.2:22516135-22516154 None:intergenic 45.0%
TGCTCTCTGCAGGAGTTTAA+GGG + chr4.2:22514633-22514652 MS.gene39485:intron 45.0%
TTCCAGATTGAAGGAGCAGA+GGG + chr4.2:22516105-22516124 MS.gene39485:CDS 45.0%
TTCTCAACATCAGTAAGCCC+TGG - chr4.2:22515434-22515453 None:intergenic 45.0%
TTCTTCTGACATCCAGTGGT+TGG - chr4.2:22513867-22513886 None:intergenic 45.0%
TTGCTCTCTGCAGGAGTTTA+AGG + chr4.2:22514632-22514651 MS.gene39485:intron 45.0%
! CGGATTTTGGATGCTCTCTT+AGG - chr4.2:22515473-22515492 None:intergenic 45.0%
! GTTGTGATTTTGCTCTCTGC+AGG + chr4.2:22514623-22514642 MS.gene39485:intron 45.0%
! TGGAGACGTTGAGGTATTTG+TGG - chr4.2:22515008-22515027 None:intergenic 45.0%
!! CCTTGGTGGTGAAAGTTCTA+CGG - chr4.2:22515550-22515569 None:intergenic 45.0%
!! CGACCTCTCTGTTTTGAACT+TGG + chr4.2:22515379-22515398 MS.gene39485:CDS 45.0%
!! GGAAGTTCTTGGTGATGCTT+TGG + chr4.2:22514522-22514541 MS.gene39485:CDS 45.0%
AACGTTGCCAATCCAACCAC+TGG + chr4.2:22513852-22513871 MS.gene39485:CDS 50.0%
ATCATGGACAGCCCTACCTT+AGG - chr4.2:22515150-22515169 None:intergenic 50.0%
CCGTAGAACTTTCACCACCA+AGG + chr4.2:22515547-22515566 MS.gene39485:CDS 50.0%
CGTAGAAGGAAGTCTGTTCG+TGG + chr4.2:22515341-22515360 MS.gene39485:CDS 50.0%
CTCTTAGGACCTCTCATCCT+TGG - chr4.2:22515458-22515477 None:intergenic 50.0%
CTGACATCCAGTGGTTGGAT+TGG - chr4.2:22513862-22513881 None:intergenic 50.0%
CTTCCAGATTGAAGGAGCAG+AGG + chr4.2:22516104-22516123 MS.gene39485:CDS 50.0%
GCTCCTTCAATCTGGAAGCA+AGG - chr4.2:22516102-22516121 None:intergenic 50.0%
GGTGGTGAAAGTTCTACGGT+AGG - chr4.2:22515546-22515565 None:intergenic 50.0%
GTCAACACTCCCTGCTTCAT+TGG - chr4.2:22514702-22514721 None:intergenic 50.0%
GTGAGAGCTTGGCTAAGAAG+AGG + chr4.2:22516137-22516156 MS.gene39485:CDS 50.0%
GTTAGGCTCTTGCTCCATAG+AGG + chr4.2:22514732-22514751 MS.gene39485:CDS 50.0%
GTTGAGAAGCCAAGGATGAG+AGG + chr4.2:22515446-22515465 MS.gene39485:CDS 50.0%
TCATGGACAGCCCTACCTTA+GGG - chr4.2:22515149-22515168 None:intergenic 50.0%
TCCTCTTGTCCCAGAAAGCA+CGG - chr4.2:22514495-22514514 None:intergenic 50.0%
TTCCCTCTGCTCCTTCAATC+TGG - chr4.2:22516110-22516129 None:intergenic 50.0%
! ACTCCTTGTTTCCGTGGACA+CGG + chr4.2:22515302-22515321 MS.gene39485:CDS 50.0%
! ACTGGTGGGTGTGACAAACA+AGG + chr4.2:22514672-22514691 MS.gene39485:CDS 50.0%
! TGGCTTAGCACTGGATAGTC+TGG - chr4.2:22516164-22516183 None:intergenic 50.0%
!! CGCTTCCTTTGGAGTGTCAA+AGG - chr4.2:22516010-22516029 None:intergenic 50.0%
CATGGACAGCCCTACCTTAG+GGG - chr4.2:22515148-22515167 None:intergenic 55.0%
CTGGGACAAGAGGATCTCAC+AGG + chr4.2:22514501-22514520 MS.gene39485:CDS 55.0%
CTTCCGTGTCCACGGAAACA+AGG - chr4.2:22515308-22515327 None:intergenic 55.0%
CTTGTTTCCGTGGACACGGA+AGG + chr4.2:22515306-22515325 MS.gene39485:CDS 55.0%
TGTGGCAGATGGCTTAGCAC+TGG - chr4.2:22516173-22516192 None:intergenic 55.0%
! GCAGGAACTCCTTGTTTCCG+TGG + chr4.2:22515296-22515315 MS.gene39485:intron 55.0%
AGCAAGAGCCTAACACGGCC+AGG - chr4.2:22514726-22514745 None:intergenic 60.0%
CAAGAGCCTAACACGGCCAG+GGG - chr4.2:22514724-22514743 None:intergenic 60.0%
CATTGCGCCTTCCGTGTCCA+CGG - chr4.2:22515316-22515335 None:intergenic 60.0%
CCATGCCATAGTGAGGGCCA+AGG + chr4.2:22515113-22515132 MS.gene39485:intron 60.0%
CCCACACCTCCCCTAAGGTA+GGG + chr4.2:22515136-22515155 MS.gene39485:intron 60.0%
CCTTGGCCCTCACTATGGCA+TGG - chr4.2:22515116-22515135 None:intergenic 60.0%
GCAAGAGCCTAACACGGCCA+GGG - chr4.2:22514725-22514744 None:intergenic 60.0%
GGAACGCCGAAGTGAGAGCT+TGG + chr4.2:22516126-22516145 MS.gene39485:CDS 60.0%
TGTTGACCCCTGGCCGTGTT+AGG + chr4.2:22514715-22514734 MS.gene39485:CDS 60.0%
! CCCTACCTTAGGGGAGGTGT+GGG - chr4.2:22515139-22515158 None:intergenic 60.0%
! GGCGCAATGGAGAGCGTAGA+AGG + chr4.2:22515327-22515346 MS.gene39485:CDS 60.0%
!! AAGCAGGGAGTGTTGACCCC+TGG + chr4.2:22514705-22514724 MS.gene39485:CDS 60.0%
!! GCCGTGCTTTCTGGGACAAG+AGG + chr4.2:22514491-22514510 MS.gene39485:intron 60.0%
CAAGGCCCACACCTCCCCTA+AGG + chr4.2:22515131-22515150 MS.gene39485:intron 65.0%
GCCCACACCTCCCCTAAGGT+AGG + chr4.2:22515135-22515154 MS.gene39485:intron 65.0%
GCCCTACCTTAGGGGAGGTG+TGG - chr4.2:22515140-22515159 None:intergenic 65.0%
GGACAGCCCTACCTTAGGGG+AGG - chr4.2:22515145-22515164 None:intergenic 65.0%
GTGGGCCTTGGCCCTCACTA+TGG - chr4.2:22515121-22515140 None:intergenic 65.0%
! CGTGGACACGGAAGGCGCAA+TGG + chr4.2:22515314-22515333 MS.gene39485:CDS 65.0%
! CTTAGGGGAGGTGTGGGCCT+TGG - chr4.2:22515133-22515152 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 22513753 22516198 22513753 ID=MS.gene39485
chr4.2 mRNA 22513753 22516198 22513753 ID=MS.gene39485.t1;Parent=MS.gene39485
chr4.2 exon 22513753 22513758 22513753 ID=MS.gene39485.t1.exon1;Parent=MS.gene39485.t1
chr4.2 CDS 22513753 22513758 22513753 ID=cds.MS.gene39485.t1;Parent=MS.gene39485.t1
chr4.2 exon 22513849 22513913 22513849 ID=MS.gene39485.t1.exon2;Parent=MS.gene39485.t1
chr4.2 CDS 22513849 22513913 22513849 ID=cds.MS.gene39485.t1;Parent=MS.gene39485.t1
chr4.2 exon 22514492 22514549 22514492 ID=MS.gene39485.t1.exon3;Parent=MS.gene39485.t1
chr4.2 CDS 22514492 22514549 22514492 ID=cds.MS.gene39485.t1;Parent=MS.gene39485.t1
chr4.2 exon 22514645 22514753 22514645 ID=MS.gene39485.t1.exon4;Parent=MS.gene39485.t1
chr4.2 CDS 22514645 22514753 22514645 ID=cds.MS.gene39485.t1;Parent=MS.gene39485.t1
chr4.2 exon 22515300 22515572 22515300 ID=MS.gene39485.t1.exon5;Parent=MS.gene39485.t1
chr4.2 CDS 22515300 22515572 22515300 ID=cds.MS.gene39485.t1;Parent=MS.gene39485.t1
chr4.2 exon 22515963 22516198 22515963 ID=MS.gene39485.t1.exon6;Parent=MS.gene39485.t1
chr4.2 CDS 22515963 22516198 22515963 ID=cds.MS.gene39485.t1;Parent=MS.gene39485.t1
Gene Sequence

>MS.gene39485

ATGAAGGTTTGTTTTCACTCTCTTCAATTCTTCGATTTCAATGTTGTTGTTGTTGTTTAATTAAACTGAATCATTGTGTTTTCGTTTTTGTTTCAGTTTAACGTTGCCAATCCAACCACTGGATGTCAGAAGAAGCTTGAAATCGATGATGACATGAAGCTGTAATAATCCATTTACTCTCTCTTCATTTTCGCATTTTTCATTTGAAGTATTTTTCTTAGGACAAAAATCATTACTTTGAAGTGACCATGTTCATAAATCTATGTTGGTTTCTGTTCTTTATGTTTGTGCCGAAATTCTTTTCAATTTTCTGTGTAATTGTTTTTTTTTTCTTTTCATTTTTATATGACTGTAGTGTAAATCTGGTACTTGTTTTCAATATGTTTTGTAATGCATGGTGAAATGAACATTGTCATTTATCTGAATTTGGTTTGAACTGATATTTGGAGCCTGTTTGGATTGGTTTGTTTAGTCGAGAGAATTTATAAAAACAACTTATGACATGTTTATAACCTGTTTCCAACTTATTTCTACAAGTTCACCAAGATAGCTTATGAAAATAGTTTATGGCTTAATACAAAAACAATCTGACTTTATTTTATCGTGTGTAGTAGGAATAGTTTATTCACAAGTGCTTGTTGTTAGTGCTTATTATAAGCACTTGTGCTATATGCTGCAAATTAAGTTGTTTATCAAAACAAGGTCTCAGTGTAGTGCTATTATCTTTTGTATATTGCAGCCGTGCTTTCTGGGACAAGAGGATCTCACAGGAAGTTCTTGGTGATGCTTTGGGAGAAGTATGATCTCTAGCATGAAATTTGCGTTGTCTACTTCCTTATTTTGTAGAGCATTGTAATTGATCTATTTTGTGTTGTGATTTTGCTCTCTGCAGGAGTTTAAGGGTTATGTCTTCAAGATCACTGGTGGGTGTGACAAACAAGGATTCCCAATGAAGCAGGGAGTGTTGACCCCTGGCCGTGTTAGGCTCTTGCTCCATAGAGGTAGCATTTTTTTTTTCTTCAAATCTTCAATCTATTTACCTTTCGGCATACTGTATGTTGCATTGAAAATTTTGCCTCTTAGTATTACTATCAGAGACGTTCATAAATTTATAGTAGTGGAGGCATTATAAGTAAAGAAACAACCATTTTACTATTTATCTCATGTGATTTTGCATTGGATTATTGGTTATATAGCATATGTAGTTATTTCAGTAGGTGCAATTTCCACGTAATATTAGAGATAACTTTAACCACAAATACCTCAACGTCTCCACTAACTTTATGAAAAGAACTTTATTCCCTCCTAGTTAAATAGTTTTGGATGTGTGGGAAACATGCATATAGTATGGAAAAAAAATCCATGCCATAGTGAGGGCCAAGGCCCACACCTCCCCTAAGGTAGGGCTGTCCATGATCACAATGATTATTGTTTGTGATGCAACACGTAATGTTTTATGTTTTTGTGCTCCACTTAGTATTTCATTATTTGCAGGAGTAAGCTTCTTTTCTACTTGGCTAATTTTTTATGCATTTTGCACTTTGCAGGAACTCCTTGTTTCCGTGGACACGGAAGGCGCAATGGAGAGCGTAGAAGGAAGTCTGTTCGTGGATGCATTGTCAGCCCCGACCTCTCTGTTTTGAACTTGGTTATTGTGAAGAAAGGTGATAATGATCTTCCAGGGCTTACTGATGTTGAGAAGCCAAGGATGAGAGGTCCTAAGAGAGCATCCAAAATCCGTAAGCTGTTCAACCTGTCAAAGGATGATGATGTGCGAAAATATGTTAACACCTACCGTAGAACTTTCACCACCAAGGATGGTAAGTTTCTGATTCCACGAATATCGTAACCATTTCTTTATATATCCTTATATTAAGGGTAGTGGGGGAGATGTTGAATTAAATTTTTTTTTTGATTAAAAAACAGTTGTCCAAACTACTAGGGGCTTCATAGCCTCCACTTGTATTCATTTTTGTTCTAGCAGAGACTTGCTGTAGTTTTCTTTGTTTTAAGTATAAAGAATATTACTTTCATTGAAGGCAGTGATACTTTACAGTATACATTGTCTAGATTGGGGTTTAGAGTACCCGTTTGAAAGAAGTGCCGTTTCCTCACAATTTGCGATTGTCCTTCAGTGACATCCGGTAAAATTGTACCAATACAGATATTTGTAATGAATTGTAATCTTAGTGGTTGTTGTTTGATTGCAGGGAAGAAGGTTAGCAAAGCTCCAAAGATACAGCGATTGGTCACTCCTTTGACACTCCAAAGGAAGCGAGCAAGAATTGCAGACAAGAAGAAGAGAATTGCTAAGGCAAAATCAGAGGCAGCAGATTATCAAAAACTCCTTGCTTCCAGATTGAAGGAGCAGAGGGAACGCCGAAGTGAGAGCTTGGCTAAGAAGAGGTCCAGACTATCCAGTGCTAAGCCATCTGCCACAGCATAA
Protein sequence

>MS.gene39485.t1

MKFNVANPTTGCQKKLEIDDDMKLRAFWDKRISQEVLGDALGEEFKGYVFKITGGCDKQGFPMKQGVLTPGRVRLLLHRGTPCFRGHGRRNGERRRKSVRGCIVSPDLSVLNLVIVKKGDNDLPGLTDVEKPRMRGPKRASKIRKLFNLSKDDDVRKYVNTYRRTFTTKDGKKVSKAPKIQRLVTPLTLQRKRARIADKKKRIAKAKSEAADYQKLLASRLKEQRERRSESLAKKRSRLSSAKPSATA