AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene08259

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08259.t1 MTR_8g080150 95.238 357 12 3 1 355 1 354 0.0 686
MS.gene08259.t1 MTR_8g080140 68.661 351 101 5 9 354 7 353 1.57e-168 474
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08259.t1 AT5G11010 56.034 348 144 5 12 355 10 352 4.63e-135 389
MS.gene08259.t1 AT5G11010 56.034 348 144 5 12 355 10 352 4.63e-135 389
MS.gene08259.t1 AT5G11010 56.034 348 144 5 12 355 10 352 4.63e-135 389
MS.gene08259.t1 AT5G11010 54.426 305 130 5 12 312 10 309 1.09e-113 333

Find 80 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAATAAACTTGTAGGAATT+TGG 0.140890 4.1:-15444205 None:intergenic
AATACCTCTGGCTGGGTTAA+AGG 0.251617 4.1:+15442469 MS.gene08259:CDS
CTTACCTGCAATAAACTTGT+AGG 0.273363 4.1:-15444212 None:intergenic
CCATTTGACCCGAACTTTCC+AGG 0.282127 4.1:-15442696 None:intergenic
CATTGCAGGTGATGGTTATA+AGG 0.288067 4.1:+15442596 MS.gene08259:intron
AAAAGTCAACCTCTTGCTTC+AGG 0.296771 4.1:+15444175 MS.gene08259:CDS
AGCCTGAATGCTAGTATTGT+TGG 0.330336 4.1:+15443189 MS.gene08259:CDS
AAGAATTTACCTGGAAAGTT+CGG 0.336338 4.1:+15442687 MS.gene08259:CDS
CTTGCGATGGGAACATTATA+AGG 0.336390 4.1:-15443026 None:intergenic
CTGATTACACCTGTGCCATT+TGG 0.342406 4.1:+15444143 MS.gene08259:CDS
CTCGGTCGCATCTAGTTAAT+AGG 0.357472 4.1:-15441105 None:intergenic
CCTGGAAAGTTCGGGTCAAA+TGG 0.360089 4.1:+15442696 MS.gene08259:CDS
CTGGAGAGTATTTCAGAGAT+TGG 0.360131 4.1:-15443168 None:intergenic
CCTCCCATCACATTTGTTTG+TGG 0.372621 4.1:+15441034 MS.gene08259:CDS
AATTCCTACAAGTTTATTGC+AGG 0.374142 4.1:+15444208 MS.gene08259:CDS
CCCCAGGGTGCCTGCTCAAA+AGG 0.383947 4.1:+15442890 MS.gene08259:intron
AGGGGCATTGACACAGTTAA+AGG 0.386318 4.1:+15444110 MS.gene08259:CDS
GAAAGAGCAATGGGTTCATC+TGG 0.388190 4.1:+15440938 None:intergenic
TTACCCTGGTGCCTTGGTCT+TGG 0.389061 4.1:+15443243 MS.gene08259:CDS
AGCCAACAATACTAGCATTC+AGG 0.390103 4.1:-15443191 None:intergenic
TTGTAGTGAATACCTCTGGC+TGG 0.403286 4.1:+15442461 MS.gene08259:CDS
GTGAACATGCTAAAATATAT+TGG 0.405008 4.1:+15442624 MS.gene08259:CDS
AGAATTTACCTGGAAAGTTC+GGG 0.410696 4.1:+15442688 MS.gene08259:CDS
TATTTATGATTATTATCATA+AGG 0.411756 4.1:+15442387 MS.gene08259:CDS
TTTCATAGTTTATACCTCTC+TGG 0.415607 4.1:-15441668 None:intergenic
CAACCTGAGTTTACTCCACC+TGG 0.435961 4.1:+15441154 MS.gene08259:CDS
TTCAGATATTTCAACAATCA+AGG 0.444136 4.1:+15442974 MS.gene08259:CDS
TGGAGAGTATTTCAGAGATT+GGG 0.453316 4.1:-15443167 None:intergenic
CCCACTCATATAGTTAAGAT+AGG 0.455843 4.1:+15442648 MS.gene08259:CDS
TGAGATATATCCAGCTCGTC+AGG 0.461392 4.1:+15442746 MS.gene08259:CDS
CTGGAAAGTTCGGGTCAAAT+GGG 0.477512 4.1:+15442697 MS.gene08259:CDS
TTTAATGAGTCCTGACGAGC+TGG 0.479415 4.1:-15442756 None:intergenic
CAGCTTGTGACCATTGTTGT+GGG 0.481609 4.1:-15440985 None:intergenic
AGGAGTTGGACTGGATGTAA+TGG 0.489408 4.1:-15441014 None:intergenic
AAAGTCAACCTCTTGCTTCA+GGG 0.498916 4.1:+15444176 MS.gene08259:CDS
ACAAATGTGATGGGAGGAGT+TGG 0.500253 4.1:-15441028 None:intergenic
GCAGCTTGTGACCATTGTTG+TGG 0.508024 4.1:-15440986 None:intergenic
TGTTGTGGGATGTAAATGTC+TGG 0.513328 4.1:-15440971 None:intergenic
CCTCTTGTAGTGAATACCTC+TGG 0.514438 4.1:+15442457 MS.gene08259:CDS
TTCAGAGAACCAAATGGCAC+AGG 0.515584 4.1:-15444152 None:intergenic
TTGTGATTCAGGTATTGTGA+GGG 0.527148 4.1:+15444091 MS.gene08259:intron
CCTACCAAGACCAAGGCACC+AGG 0.529524 4.1:-15443247 None:intergenic
TCGAATCTTTATGCTTGCGA+TGG 0.529810 4.1:-15443039 None:intergenic
GCTTATCTTGAAACTGATGT+TGG 0.537454 4.1:+15441130 MS.gene08259:CDS
TGTAGTGAATACCTCTGGCT+GGG 0.538425 4.1:+15442462 MS.gene08259:CDS
TGCTCCACAAACAAATGTGA+TGG 0.541521 4.1:-15441038 None:intergenic
ATTGTTAATAAAGTTACTCC+AGG 0.544370 4.1:+15441193 MS.gene08259:CDS
AACAATACTAGCATTCAGGC+TGG 0.547070 4.1:-15443187 None:intergenic
AGGTAAATTCTTCAGATCAG+TGG 0.550384 4.1:-15442676 None:intergenic
CTTGTGATTCAGGTATTGTG+AGG 0.553596 4.1:+15444090 MS.gene08259:intron
CGAATCTTTATGCTTGCGAT+GGG 0.557731 4.1:-15443038 None:intergenic
GGCTTAGCAGTTGAATCTGA+AGG 0.565104 4.1:+15443210 MS.gene08259:CDS
ATAAGGACAGTGAGAGGTCA+AGG 0.568437 4.1:-15443009 None:intergenic
ATTGAGGAGATTTCGCGAGA+AGG 0.573264 4.1:-15441080 None:intergenic
TGTGATTCAGGTATTGTGAG+GGG 0.578885 4.1:+15444092 MS.gene08259:intron
TGGAGTAACTTTATTAACAA+TGG 0.579021 4.1:-15441191 None:intergenic
GCCTATCTTAACTATATGAG+TGG 0.583863 4.1:-15442649 None:intergenic
ACTGATCTGAAGAATTTACC+TGG 0.591590 4.1:+15442678 MS.gene08259:CDS
TGTGATGGGAGGAGTTGGAC+TGG 0.596371 4.1:-15441023 None:intergenic
CCAGAGGTATTCACTACAAG+AGG 0.598694 4.1:-15442457 None:intergenic
GGATATTACCCTGAAGCAAG+AGG 0.598881 4.1:-15444184 None:intergenic
GCTCCACAAACAAATGTGAT+GGG 0.614241 4.1:-15441037 None:intergenic
ATCTAGTTAATAGGACATTG+AGG 0.620697 4.1:-15441096 None:intergenic
AAACCAGGTGGAGTAAACTC+AGG 0.622096 4.1:-15441157 None:intergenic
ATGGTAAGAGAGATAAAACC+AGG 0.622345 4.1:-15441172 None:intergenic
CAGTTTCAAGATAAGCAACT+CGG 0.625840 4.1:-15441123 None:intergenic
CAGGTGGAGTAAACTCAGGT+TGG 0.625964 4.1:-15441153 None:intergenic
CCTATCTTAACTATATGAGT+GGG 0.628881 4.1:-15442648 None:intergenic
GTTGAAATATCTGAATCACT+AGG 0.636570 4.1:-15442966 None:intergenic
TTTACCTTTAACCCAGCCAG+AGG 0.641811 4.1:-15442473 None:intergenic
AACATTATAAGGACAGTGAG+AGG 0.648592 4.1:-15443015 None:intergenic
GATTCGACGTGTTCTTCACG+AGG 0.658346 4.1:+15443055 MS.gene08259:CDS
GTGGGATGTAAATGTCTGGA+AGG 0.660248 4.1:-15440967 None:intergenic
ACATTTACATCCCACAACAA+TGG 0.661470 4.1:+15440975 MS.gene08259:CDS
CCACAAACAAATGTGATGGG+AGG 0.666150 4.1:-15441034 None:intergenic
GTTTGTGGAGCAAGTAACTG+CGG 0.670641 4.1:+15441049 MS.gene08259:CDS
CAAGTTACCTACCAAGACCA+AGG 0.684244 4.1:-15443254 None:intergenic
CTACCAAGACCAAGGCACCA+GGG 0.712305 4.1:-15443246 None:intergenic
GTAAGAGAGATAAAACCAGG+TGG 0.719648 4.1:-15441169 None:intergenic
CATGCCTGAAAACACCAGAG+AGG 0.772404 4.1:+15441654 MS.gene08259:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATTGATATATTGTTAT+TGG - chr4.1:15442547-15442566 None:intergenic 10.0%
!! TATTTATGATTATTATCATA+AGG + chr4.1:15442387-15442406 MS.gene08259:CDS 10.0%
!! ATTATTGAATCAATGAATAT+AGG - chr4.1:15441559-15441578 None:intergenic 15.0%
!! TGAATAAAGTTACAAAATAA+AGG + chr4.1:15443495-15443514 MS.gene08259:intron 15.0%
!! TTATTGAATCAATGAATATA+GGG - chr4.1:15441558-15441577 None:intergenic 15.0%
!! TTCTATAAAAATGTTCATTA+TGG + chr4.1:15441580-15441599 MS.gene08259:intron 15.0%
!! ATATCTCATCTCAATAATTT+GGG + chr4.1:15442065-15442084 MS.gene08259:intron 20.0%
!! TGTAAACAAAAAACAACATT+TGG + chr4.1:15443722-15443741 MS.gene08259:intron 20.0%
!! TTAACAAGATACAATAGTTA+AGG - chr4.1:15441815-15441834 None:intergenic 20.0%
!! TTACATGTAAATGTTAGAAT+AGG - chr4.1:15441237-15441256 None:intergenic 20.0%
!!! AAATGTTGTTTTTTGTTTAC+AGG - chr4.1:15443723-15443742 None:intergenic 20.0%
!!! ATATTTGATTCTTTTAGTGT+CGG + chr4.1:15442216-15442235 MS.gene08259:intron 20.0%
!!! ATGTATTTTAAGTATGTTGA+CGG - chr4.1:15442361-15442380 None:intergenic 20.0%
!!! ATTATATCTCATATTTCTGT+TGG + chr4.1:15443847-15443866 MS.gene08259:intron 20.0%
!!! TGACTTATGAATATTTTTGT+AGG + chr4.1:15443143-15443162 MS.gene08259:intron 20.0%
!!! TTCTTTATCTTTTCTATTTG+TGG - chr4.1:15443941-15443960 None:intergenic 20.0%
! AAGAAACATCACCAAAAAAA+AGG - chr4.1:15442327-15442346 None:intergenic 25.0%
! AGAAACATCACCAAAAAAAA+GGG - chr4.1:15442326-15442345 None:intergenic 25.0%
! AGAATAAATTCATATTCCTC+TGG + chr4.1:15443957-15443976 MS.gene08259:intron 25.0%
! ATTCATAGAGTTGAATCATT+TGG - chr4.1:15443480-15443499 None:intergenic 25.0%
! ATTGTTAATAAAGTTACTCC+AGG + chr4.1:15441193-15441212 MS.gene08259:CDS 25.0%
! CTTGATATCATTCAAACTTA+TGG + chr4.1:15443777-15443796 MS.gene08259:intron 25.0%
! GATATCTCATCTCAATAATT+TGG + chr4.1:15442064-15442083 MS.gene08259:intron 25.0%
! GGATGTTTGATACATTAAAA+AGG - chr4.1:15443920-15443939 None:intergenic 25.0%
! GTGAACATGCTAAAATATAT+TGG + chr4.1:15442624-15442643 MS.gene08259:CDS 25.0%
! TAAAAGGAATTTGCATTTGT+AGG + chr4.1:15443816-15443835 MS.gene08259:intron 25.0%
! TGGAGTAACTTTATTAACAA+TGG - chr4.1:15441194-15441213 None:intergenic 25.0%
! TTAATGACATTGATACAAAC+TGG - chr4.1:15442024-15442043 None:intergenic 25.0%
! TTCAGATATTTCAACAATCA+AGG + chr4.1:15442974-15442993 MS.gene08259:CDS 25.0%
! TTCTTAATAGCATAGATTTG+AGG + chr4.1:15441897-15441916 MS.gene08259:intron 25.0%
!! AATTCTTTTATCTATGTGAG+TGG - chr4.1:15441929-15441948 None:intergenic 25.0%
!! ACGAGATTTGATAATTATGT+TGG + chr4.1:15441514-15441533 MS.gene08259:intron 25.0%
!! AGAATTTTGTCTCTTATCAA+AGG - chr4.1:15442422-15442441 None:intergenic 25.0%
!! CGAGATTTGATAATTATGTT+GGG + chr4.1:15441515-15441534 MS.gene08259:intron 25.0%
!!! AAACTTGTCACTTTTTTCAT+GGG + chr4.1:15441483-15441502 MS.gene08259:intron 25.0%
!!! TATTTGTTGCTTTGAATTTG+AGG + chr4.1:15442113-15442132 MS.gene08259:intron 25.0%
AAGAATTTACCTGGAAAGTT+CGG + chr4.1:15442687-15442706 MS.gene08259:CDS 30.0%
AAGCTGATAATTATGTACGA+CGG - chr4.1:15441285-15441304 None:intergenic 30.0%
AATACCTATTAATCAGCTTG+AGG - chr4.1:15443579-15443598 None:intergenic 30.0%
AATTCCTACAAGTTTATTGC+AGG + chr4.1:15444208-15444227 MS.gene08259:CDS 30.0%
AGATACAATAGTTAAGGTTG+AGG - chr4.1:15441809-15441828 None:intergenic 30.0%
AGGATATTAGTTACATACAC+AGG + chr4.1:15443515-15443534 MS.gene08259:intron 30.0%
ATACCTATTAATCAGCTTGA+GGG - chr4.1:15443578-15443597 None:intergenic 30.0%
ATCTAGTTAATAGGACATTG+AGG - chr4.1:15441099-15441118 None:intergenic 30.0%
ATGTCATTAACATGTGATAC+TGG + chr4.1:15442034-15442053 MS.gene08259:intron 30.0%
CACGTAGTAAAACTAAATGT+GGG - chr4.1:15441359-15441378 None:intergenic 30.0%
CAGAATATTGTATACAAGCT+GGG - chr4.1:15443609-15443628 None:intergenic 30.0%
CATTCAAATAAGACATGGAT+TGG - chr4.1:15442242-15442261 None:intergenic 30.0%
CCTATCTTAACTATATGAGT+GGG - chr4.1:15442651-15442670 None:intergenic 30.0%
CTTAGCATCATTACTGTTAA+AGG + chr4.1:15444056-15444075 MS.gene08259:intron 30.0%
GAGAAAAGCAGTAGTTTATT+TGG - chr4.1:15442522-15442541 None:intergenic 30.0%
GCAATAAACTTGTAGGAATT+TGG - chr4.1:15444208-15444227 None:intergenic 30.0%
GCATAACAATGTGACAAAAT+AGG - chr4.1:15442140-15442159 None:intergenic 30.0%
GCATTCATTCAAATAAGACA+TGG - chr4.1:15442247-15442266 None:intergenic 30.0%
GTTGAAATATCTGAATCACT+AGG - chr4.1:15442969-15442988 None:intergenic 30.0%
TATATGTTAGAAAACCTACC+TGG - chr4.1:15441214-15441233 None:intergenic 30.0%
TTAATAAAGTTACTCCAGGT+AGG + chr4.1:15441197-15441216 MS.gene08259:intron 30.0%
TTATTACTTTGAGCTACCAA+AGG + chr4.1:15444308-15444327 MS.gene08259:intron 30.0%
TTTCATAGTTTATACCTCTC+TGG - chr4.1:15441671-15441690 None:intergenic 30.0%
! TTTGTCTGTTTGTAGTTTGT+AGG + chr4.1:15442191-15442210 MS.gene08259:intron 30.0%
!! GTATACAATATTCTGGAACA+TGG + chr4.1:15443614-15443633 MS.gene08259:intron 30.0%
!! GTTTAAAATTTTCAAGCACG+TGG - chr4.1:15441457-15441476 None:intergenic 30.0%
!! TTCTGATCGAAAGTATCTTA+AGG - chr4.1:15441968-15441987 None:intergenic 30.0%
!!! ACTTTTTTCAGAGAACCAAA+TGG - chr4.1:15444161-15444180 None:intergenic 30.0%
!!! CAAACTTGTCACTTTTTTCA+TGG + chr4.1:15441482-15441501 MS.gene08259:intron 30.0%
!!! GTTTTGATTTCTTGTGATTC+AGG + chr4.1:15444080-15444099 MS.gene08259:intron 30.0%
!!! TGTTTTTTGTTTACAGGCAT+TGG - chr4.1:15443717-15443736 None:intergenic 30.0%
AACATTATAAGGACAGTGAG+AGG - chr4.1:15443018-15443037 None:intergenic 35.0%
ACATTTACATCCCACAACAA+TGG + chr4.1:15440975-15440994 MS.gene08259:CDS 35.0%
ACCAAAGGAAAACTTTCTGT+TGG + chr4.1:15444323-15444342 MS.gene08259:intron 35.0%
ACTAAACTAGCAGCTGAAAA+TGG - chr4.1:15442811-15442830 None:intergenic 35.0%
ACTGATCTGAAGAATTTACC+TGG + chr4.1:15442678-15442697 MS.gene08259:CDS 35.0%
AGAATTTACCTGGAAAGTTC+GGG + chr4.1:15442688-15442707 MS.gene08259:CDS 35.0%
AGGTAAATTCTTCAGATCAG+TGG - chr4.1:15442679-15442698 None:intergenic 35.0%
ATGACAGCCAGAAAATTAGA+TGG - chr4.1:15444022-15444041 None:intergenic 35.0%
ATGGTAAGAGAGATAAAACC+AGG - chr4.1:15441175-15441194 None:intergenic 35.0%
ATTCCAAAATGTCTCGTCAT+AGG + chr4.1:15443289-15443308 MS.gene08259:intron 35.0%
CAGTTTCAAGATAAGCAACT+CGG - chr4.1:15441126-15441145 None:intergenic 35.0%
CATAGAGTTGAATCATTTGG+AGG - chr4.1:15443477-15443496 None:intergenic 35.0%
CATAGTTTATACATCTCTCG+TGG - chr4.1:15441773-15441792 None:intergenic 35.0%
CATGTACATAGAACACTCAT+TGG - chr4.1:15443370-15443389 None:intergenic 35.0%
CCAGAATATTGTATACAAGC+TGG - chr4.1:15443610-15443629 None:intergenic 35.0%
CCAGCTTGTATACAATATTC+TGG + chr4.1:15443607-15443626 MS.gene08259:intron 35.0%
CCCACTCATATAGTTAAGAT+AGG + chr4.1:15442648-15442667 MS.gene08259:CDS 35.0%
CTTACCTGCAATAAACTTGT+AGG - chr4.1:15444215-15444234 None:intergenic 35.0%
GCACGTAGTAAAACTAAATG+TGG - chr4.1:15441360-15441379 None:intergenic 35.0%
GCCTATCTTAACTATATGAG+TGG - chr4.1:15442652-15442671 None:intergenic 35.0%
GCTTATCTTGAAACTGATGT+TGG + chr4.1:15441130-15441149 MS.gene08259:CDS 35.0%
TAATGTGGTCAACTAGTTGA+AGG + chr4.1:15443658-15443677 MS.gene08259:intron 35.0%
TACCTATTAATCAGCTTGAG+GGG - chr4.1:15443577-15443596 None:intergenic 35.0%
TAGGGAATCTCAAACATCAT+CGG - chr4.1:15443440-15443459 None:intergenic 35.0%
TAGTTTCTTGTTCTGCCTTA+GGG - chr4.1:15443396-15443415 None:intergenic 35.0%
TGAGTTGCAACACATCTTAA+TGG + chr4.1:15443887-15443906 MS.gene08259:intron 35.0%
TTAGTTTCTTGTTCTGCCTT+AGG - chr4.1:15443397-15443416 None:intergenic 35.0%
! ACCAACAGAAAGTTTTCCTT+TGG - chr4.1:15444327-15444346 None:intergenic 35.0%
! ATATTTCTGTTGGCTGTAAC+AGG + chr4.1:15443857-15443876 MS.gene08259:intron 35.0%
! ATGCTACTTATCATCTCTTC+TGG + chr4.1:15443087-15443106 MS.gene08259:intron 35.0%
! CATACAGCCATCTAATTTTC+TGG + chr4.1:15444012-15444031 MS.gene08259:intron 35.0%
! TCAATTCTTGACTTGACCAA+TGG + chr4.1:15441839-15441858 MS.gene08259:intron 35.0%
! TTGTGATTCAGGTATTGTGA+GGG + chr4.1:15444091-15444110 MS.gene08259:intron 35.0%
!! TGGAGAGTATTTCAGAGATT+GGG - chr4.1:15443170-15443189 None:intergenic 35.0%
!!! CACTCATTGGTGTTTTGTTT+AGG - chr4.1:15443357-15443376 None:intergenic 35.0%
!!! CGTAACAGATCCCTTTTTTT+TGG + chr4.1:15442313-15442332 MS.gene08259:intron 35.0%
!!! CTTTCTGTTGGTTTTTCCTT+AGG + chr4.1:15444335-15444354 MS.gene08259:intron 35.0%
!!! TGACGAGACATTTTGGAATT+TGG - chr4.1:15443288-15443307 None:intergenic 35.0%
AAAAGTCAACCTCTTGCTTC+AGG + chr4.1:15444175-15444194 MS.gene08259:CDS 40.0%
AAAGTCAACCTCTTGCTTCA+GGG + chr4.1:15444176-15444195 MS.gene08259:CDS 40.0%
AACAATACTAGCATTCAGGC+TGG - chr4.1:15443190-15443209 None:intergenic 40.0%
AAGGACCTAAAAAGTTACCC+TGG + chr4.1:15443229-15443248 MS.gene08259:CDS 40.0%
ACCTATTAATCAGCTTGAGG+GGG - chr4.1:15443576-15443595 None:intergenic 40.0%
AGCCAACAATACTAGCATTC+AGG - chr4.1:15443194-15443213 None:intergenic 40.0%
AGCCTGAATGCTAGTATTGT+TGG + chr4.1:15443189-15443208 MS.gene08259:CDS 40.0%
ATATCCCTATTTGTTTCCCC+AGG + chr4.1:15442874-15442893 MS.gene08259:intron 40.0%
ATCTTGTAATGACGAGCACA+TGG + chr4.1:15443987-15444006 MS.gene08259:intron 40.0%
ATGAATGCGCTCACTTGTAA+TGG + chr4.1:15442259-15442278 MS.gene08259:intron 40.0%
CGAATCTTTATGCTTGCGAT+GGG - chr4.1:15443041-15443060 None:intergenic 40.0%
CTTGCGATGGGAACATTATA+AGG - chr4.1:15443029-15443048 None:intergenic 40.0%
GCTCCACAAACAAATGTGAT+GGG - chr4.1:15441040-15441059 None:intergenic 40.0%
GTAAGAGAGATAAAACCAGG+TGG - chr4.1:15441172-15441191 None:intergenic 40.0%
GTCTTCTACAAACAGTGAGT+TGG + chr4.1:15442161-15442180 MS.gene08259:intron 40.0%
TAGCAGTGAGGTACACTAAA+AGG + chr4.1:15443800-15443819 MS.gene08259:intron 40.0%
TATCCCTATTTGTTTCCCCA+GGG + chr4.1:15442875-15442894 MS.gene08259:intron 40.0%
TCAAACTTATGGTAGCAGTG+AGG + chr4.1:15443788-15443807 MS.gene08259:intron 40.0%
TCGAATCTTTATGCTTGCGA+TGG - chr4.1:15443042-15443061 None:intergenic 40.0%
TCTATGTACATGAGACCCTA+AGG + chr4.1:15443378-15443397 MS.gene08259:intron 40.0%
TGCTCCACAAACAAATGTGA+TGG - chr4.1:15441041-15441060 None:intergenic 40.0%
TGTTGTGGGATGTAAATGTC+TGG - chr4.1:15440974-15440993 None:intergenic 40.0%
TTACAAGATCAGAAGACCAG+AGG - chr4.1:15443976-15443995 None:intergenic 40.0%
TTGACCACATTACTGCCAAA+GGG - chr4.1:15443650-15443669 None:intergenic 40.0%
! CAACCTATGACGAGACATTT+TGG - chr4.1:15443295-15443314 None:intergenic 40.0%
! CATTGCAGGTGATGGTTATA+AGG + chr4.1:15442596-15442615 MS.gene08259:intron 40.0%
! CTTGTGATTCAGGTATTGTG+AGG + chr4.1:15444090-15444109 MS.gene08259:intron 40.0%
! GTAATTGAGTCTGAGACCAT+TGG - chr4.1:15441858-15441877 None:intergenic 40.0%
! TATACCTCTCTGGTGTTTTC+AGG - chr4.1:15441661-15441680 None:intergenic 40.0%
! TGTGATTCAGGTATTGTGAG+GGG + chr4.1:15444092-15444111 MS.gene08259:intron 40.0%
! TGTGTTTTGTCTTGCATTGC+AGG + chr4.1:15442582-15442601 MS.gene08259:intron 40.0%
!! CTGGAGAGTATTTCAGAGAT+TGG - chr4.1:15443171-15443190 None:intergenic 40.0%
!!! CTTGTCACTTTTTTCATGGG+AGG + chr4.1:15441486-15441505 MS.gene08259:intron 40.0%
AAAAAGTTACCCTGGTGCCT+TGG + chr4.1:15443237-15443256 MS.gene08259:CDS 45.0%
AAACCAGGTGGAGTAAACTC+AGG - chr4.1:15441160-15441179 None:intergenic 45.0%
AATACCTCTGGCTGGGTTAA+AGG + chr4.1:15442469-15442488 MS.gene08259:CDS 45.0%
ATAAGGACAGTGAGAGGTCA+AGG - chr4.1:15443012-15443031 None:intergenic 45.0%
ATTGAGGAGATTTCGCGAGA+AGG - chr4.1:15441083-15441102 None:intergenic 45.0%
ATTGGAAGCCTTCTACACGA+AGG - chr4.1:15443699-15443718 None:intergenic 45.0%
CAAGTTACCTACCAAGACCA+AGG - chr4.1:15443257-15443276 None:intergenic 45.0%
CAGCTTGTGACCATTGTTGT+GGG - chr4.1:15440988-15441007 None:intergenic 45.0%
CCTCCCATCACATTTGTTTG+TGG + chr4.1:15441034-15441053 MS.gene08259:CDS 45.0%
CCTCTTGTAGTGAATACCTC+TGG + chr4.1:15442457-15442476 MS.gene08259:CDS 45.0%
CTCGGTCGCATCTAGTTAAT+AGG - chr4.1:15441108-15441127 None:intergenic 45.0%
CTGGAAAGTTCGGGTCAAAT+GGG + chr4.1:15442697-15442716 MS.gene08259:CDS 45.0%
GGATATTACCCTGAAGCAAG+AGG - chr4.1:15444187-15444206 None:intergenic 45.0%
GGCTTAGCAGTTGAATCTGA+AGG + chr4.1:15443210-15443229 MS.gene08259:CDS 45.0%
GTGGGATGTAAATGTCTGGA+AGG - chr4.1:15440970-15440989 None:intergenic 45.0%
GTTGACCACATTACTGCCAA+AGG - chr4.1:15443651-15443670 None:intergenic 45.0%
GTTTGTGGAGCAAGTAACTG+CGG + chr4.1:15441049-15441068 MS.gene08259:CDS 45.0%
TACACGAAGGCCGCAAAATA+GGG - chr4.1:15443686-15443705 None:intergenic 45.0%
TCATTTGGAGGACTCGTTAG+TGG - chr4.1:15443465-15443484 None:intergenic 45.0%
TCCCCCTCAAGCTGATTAAT+AGG + chr4.1:15443572-15443591 MS.gene08259:intron 45.0%
TGAGATATATCCAGCTCGTC+AGG + chr4.1:15442746-15442765 MS.gene08259:CDS 45.0%
TGTAGTGAATACCTCTGGCT+GGG + chr4.1:15442462-15442481 MS.gene08259:CDS 45.0%
TTCAGAGAACCAAATGGCAC+AGG - chr4.1:15444155-15444174 None:intergenic 45.0%
TTGTAGTGAATACCTCTGGC+TGG + chr4.1:15442461-15442480 MS.gene08259:CDS 45.0%
TTGTCTTGCATTGCAGGTGA+TGG + chr4.1:15442588-15442607 MS.gene08259:intron 45.0%
TTTAATGAGTCCTGACGAGC+TGG - chr4.1:15442759-15442778 None:intergenic 45.0%
TTTACCTTTAACCCAGCCAG+AGG - chr4.1:15442476-15442495 None:intergenic 45.0%
! ACAAATGTGATGGGAGGAGT+TGG - chr4.1:15441031-15441050 None:intergenic 45.0%
! AGGAGTTGGACTGGATGTAA+TGG - chr4.1:15441017-15441036 None:intergenic 45.0%
! AGGCACCAGGGTAACTTTTT+AGG - chr4.1:15443237-15443256 None:intergenic 45.0%
! CCACAAACAAATGTGATGGG+AGG - chr4.1:15441037-15441056 None:intergenic 45.0%
! CTGATTACACCTGTGCCATT+TGG + chr4.1:15444143-15444162 MS.gene08259:CDS 45.0%
! GGGAACTGAGACTGTCTTTT+TGG + chr4.1:15442085-15442104 MS.gene08259:intron 45.0%
!! AGGGGCATTGACACAGTTAA+AGG + chr4.1:15444110-15444129 MS.gene08259:CDS 45.0%
!! CCAGAGGTATTCACTACAAG+AGG - chr4.1:15442460-15442479 None:intergenic 45.0%
!! CTTGGTCTTGGTAGGTAACT+TGG + chr4.1:15443255-15443274 MS.gene08259:intron 45.0%
!!! TTTTTAAATTTAAAAGTTTA+AGG + chr4.1:15441395-15441414 MS.gene08259:intron 5.0%
ACACGAAGGCCGCAAAATAG+GGG - chr4.1:15443685-15443704 None:intergenic 50.0%
CAACCTGAGTTTACTCCACC+TGG + chr4.1:15441154-15441173 MS.gene08259:CDS 50.0%
CAGGTGGAGTAAACTCAGGT+TGG - chr4.1:15441156-15441175 None:intergenic 50.0%
CATGCCTGAAAACACCAGAG+AGG + chr4.1:15441654-15441673 MS.gene08259:CDS 50.0%
CCATTTGACCCGAACTTTCC+AGG - chr4.1:15442699-15442718 None:intergenic 50.0%
CCTGGAAAGTTCGGGTCAAA+TGG + chr4.1:15442696-15442715 MS.gene08259:CDS 50.0%
CTACACGAAGGCCGCAAAAT+AGG - chr4.1:15443687-15443706 None:intergenic 50.0%
GAGGACTCGTTAGTGGCTTA+GGG - chr4.1:15443458-15443477 None:intergenic 50.0%
GATTCGACGTGTTCTTCACG+AGG + chr4.1:15443055-15443074 MS.gene08259:CDS 50.0%
GCACCCTGGGGAAACAAATA+GGG - chr4.1:15442881-15442900 None:intergenic 50.0%
GCAGCTTGTGACCATTGTTG+TGG - chr4.1:15440989-15441008 None:intergenic 50.0%
TTGGCCCTTTGGCAGTAATG+TGG + chr4.1:15443643-15443662 MS.gene08259:intron 50.0%
! AGACGAGCATCCTTTTGAGC+AGG - chr4.1:15442903-15442922 None:intergenic 50.0%
! GTTGAAGGCCCCCTATTTTG+CGG + chr4.1:15443673-15443692 MS.gene08259:intron 50.0%
! TTTTGCGGCCTTCGTGTAGA+AGG + chr4.1:15443688-15443707 MS.gene08259:intron 50.0%
!! CTCTCTGGTGTTTTCAGGCA+TGG - chr4.1:15441656-15441675 None:intergenic 50.0%
!! TTCTGGAACATGGTCCTGCT+TGG + chr4.1:15443624-15443643 MS.gene08259:intron 50.0%
CACGAAGGCCGCAAAATAGG+GGG - chr4.1:15443684-15443703 None:intergenic 55.0%
GGAGGACTCGTTAGTGGCTT+AGG - chr4.1:15443459-15443478 None:intergenic 55.0%
GGCACCCTGGGGAAACAAAT+AGG - chr4.1:15442882-15442901 None:intergenic 55.0%
TTACCCTGGTGCCTTGGTCT+TGG + chr4.1:15443243-15443262 MS.gene08259:CDS 55.0%
TTACTGCCAAAGGGCCAAGC+AGG - chr4.1:15443641-15443660 None:intergenic 55.0%
! ATCCTTTTGAGCAGGCACCC+TGG - chr4.1:15442895-15442914 None:intergenic 55.0%
! CTACCAAGACCAAGGCACCA+GGG - chr4.1:15443249-15443268 None:intergenic 55.0%
! TCCTTTTGAGCAGGCACCCT+GGG - chr4.1:15442894-15442913 None:intergenic 55.0%
! TGTGATGGGAGGAGTTGGAC+TGG - chr4.1:15441026-15441045 None:intergenic 55.0%
CATGGTCCTGCTTGGCCCTT+TGG + chr4.1:15443632-15443651 MS.gene08259:intron 60.0%
! CCTACCAAGACCAAGGCACC+AGG - chr4.1:15443250-15443269 None:intergenic 60.0%
! CCTTTTGAGCAGGCACCCTG+GGG - chr4.1:15442893-15442912 None:intergenic 60.0%
!! CCTGGTGCCTTGGTCTTGGT+AGG + chr4.1:15443247-15443266 MS.gene08259:intron 60.0%
! CCCCAGGGTGCCTGCTCAAA+AGG + chr4.1:15442890-15442909 MS.gene08259:intron 65.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 15440947 15444365 15440947 ID=MS.gene08259
chr4.1 mRNA 15440947 15444365 15440947 ID=MS.gene08259.t1;Parent=MS.gene08259
chr4.1 exon 15440947 15441214 15440947 ID=MS.gene08259.t1.exon1;Parent=MS.gene08259.t1
chr4.1 CDS 15440947 15441214 15440947 ID=cds.MS.gene08259.t1;Parent=MS.gene08259.t1
chr4.1 exon 15441642 15441675 15441642 ID=MS.gene08259.t1.exon2;Parent=MS.gene08259.t1
chr4.1 CDS 15441642 15441675 15441642 ID=cds.MS.gene08259.t1;Parent=MS.gene08259.t1
chr4.1 exon 15442321 15442490 15442321 ID=MS.gene08259.t1.exon3;Parent=MS.gene08259.t1
chr4.1 CDS 15442321 15442490 15442321 ID=cds.MS.gene08259.t1;Parent=MS.gene08259.t1
chr4.1 exon 15442604 15442784 15442604 ID=MS.gene08259.t1.exon4;Parent=MS.gene08259.t1
chr4.1 CDS 15442604 15442784 15442604 ID=cds.MS.gene08259.t1;Parent=MS.gene08259.t1
chr4.1 exon 15442896 15443076 15442896 ID=MS.gene08259.t1.exon5;Parent=MS.gene08259.t1
chr4.1 CDS 15442896 15443076 15442896 ID=cds.MS.gene08259.t1;Parent=MS.gene08259.t1
chr4.1 exon 15443165 15443264 15443165 ID=MS.gene08259.t1.exon6;Parent=MS.gene08259.t1
chr4.1 CDS 15443165 15443264 15443165 ID=cds.MS.gene08259.t1;Parent=MS.gene08259.t1
chr4.1 exon 15444102 15444229 15444102 ID=MS.gene08259.t1.exon7;Parent=MS.gene08259.t1
chr4.1 CDS 15444102 15444229 15444102 ID=cds.MS.gene08259.t1;Parent=MS.gene08259.t1
chr4.1 exon 15444357 15444365 15444357 ID=MS.gene08259.t1.exon8;Parent=MS.gene08259.t1
chr4.1 CDS 15444357 15444365 15444357 ID=cds.MS.gene08259.t1;Parent=MS.gene08259.t1
Gene Sequence

>MS.gene08259

ATGGGTTCATCTGGTTTTGTCCTTCCAGACATTTACATCCCACAACAATGGTCACAAGCTGCTAACTCCATTACATCCAGTCCAACTCCTCCCATCACATTTGTTTGTGGAGCAAGTAACTGCGGCAAATCAACCTTCTCGCGAAATCTCCTCAATGTCCTATTAACTAGATGCGACCGAGTTGCTTATCTTGAAACTGATGTTGGCCAACCTGAGTTTACTCCACCTGGTTTTATCTCTCTTACCATTGTTAATAAAGTTACTCCAGGTAGGTTTTCTAACATATACCTATTCTAACATTTACATGTAATAGAATCACAAGTTAAATAAGTTTTCCGTCGTACATAATTATCAGCTTTCGTTTTAATGTTCTCGTAAAAATTGTTGCATGTTTGTGTCTAATAAAATTCCCACATTTAGTTTTACTACGTGCTAAATTCAAATTTGTTTTTTAAATTTAAAAGTTTAAGGAAAATTAAACAAATTTGAATTTAACATGAATTGAAACCACGTGCTTGAAAATTTTAAACAGACGCAAACTTGTCACTTTTTTCATGGGAGGAAGAAACGAGATTTGATAATTATGTTGGGACAACAATCATATTTAACCCTATATTCATTGATTCAATAATTTTCTATAAAAATGTTCATTATGGATATTGACACGAGTTTTTTTTGTGTTTTTACTATGTTAGATTTGACGATTCCATGCCTGAAAACACCAGAGAGGTATAAACTATGAAATGTTATCTCATATCTTGATTAACTTTGTACTAATGATTAGTTAACTTTATACTAATGATGTAAATCAAATATTTTGAAACCACGAGAGATGTATAAACTATGAAATTTGATGTTGCCTCAACCTTAACTATTGTATCTTGTTAAATAATCAATTCTTGACTTGACCAATGGTCTCAGACTCAATTACTAGTTTAGATATTGTCAGATTCTTAATAGCATAGATTTGAGGCTTTTGCCACTCACATAGATAAAAGAATTGTGAATAGTTTACATGCCTTAAGATACTTTCGATCAGAAATTTTCGCAACAGTGCTAGTTTCAGTATCAATACCAGTTTGTATCAATGTCATTAACATGTGATACTGGCACTCATGATATCTCATCTCAATAATTTGGGAACTGAGACTGTCTTTTTGGTTTTTTATTTGTTGCTTTGAATTTGAGGTCCTATTTTGTCACATTGTTATGCAGTCTTCTACAAACAGTGAGTTGGATGAAATTTTGTCTGTTTGTAGTTTGTAGGATATATTTGATTCTTTTAGTGTCGGCCAATCCATGTCTTATTTGAATGAATGCGCTCACTTGTAATGGAGTTGCTTGACTCACTTTCTGTCGTTTATTGCGTAACAGATCCCTTTTTTTTGGTGATGTTTCTTCTCAGAAAGATCCGTCAACATACTTAAAATACATATGTTCTATTTATGATTATTATCATAAGGAGTATTGCACCTTTGATAAGAGACAAAATTCTTCTAAGATTCACTTGCCTCTTGTAGTGAATACCTCTGGCTGGGTTAAAGGTAAACTCGTATATTTTGTATTTTTACTCCAAATAAACTACTGCTTTTCTCGACCAATAACAATATATCAATTATTATGTTACTCACTCAATGTGTTTTGTCTTGCATTGCAGGTGATGGTTATAAGGTATTAGTGAACATGCTAAAATATATTGGTCCCACTCATATAGTTAAGATAGGCATATCCACTGATCTGAAGAATTTACCTGGAAAGTTCGGGTCAAATGGGAAGAATAACAGAAGAGTTAAATTGATTGAGATATATCCAGCTCGTCAGGACTCATTAAATAAATCGTAAGCACAAATAATATAAGTTACCATTTTCAGCTGCTAGTTTAGTTCATTATATTTTAGACTTAATTTAAGTATTACAGAATAACAGAATATCCCTATTTGTTTCCCCAGGGTGCCTGCTCAAAAGGATGCTCGTCTTGTGCGCGATTCACGAATAATAGCTTATTTCAGACAGTGCTTTCCTAGTGATTCAGATATTTCAACAATCAAGGATCTTGCAAATTCCTTGACCTCTCACTGTCCTTATAATGTTCCCATCGCAAGCATAAAGATTCGACGTGTTCTTCACGAGGTTAGTTCTAATGCTACTTATCATCTCTTCTGGTTCTGTCTCATTCATTGATCAAAACATTTTACATGACTTATGAATATTTTTGTAGGTCCCAATCTCTGAAATACTCTCCAGCCTGAATGCTAGTATTGTTGGCTTAGCAGTTGAATCTGAAGGACCTAAAAAGTTACCCTGGTGCCTTGGTCTTGGTAGGTAACTTGGTTGACTTCCAAATTCCAAAATGTCTCGTCATAGGTTGATTTCTCAAAATTTAAAAGAATATGAATTATTTATTCATCCTAAACAAAACACCAATGAGTGTTCTATGTACATGAGACCCTAAGGCAGAACAAGAAACTAATTAATGAATAATGACTAAATCCGATGATGTTTGAGATTCCCTAAGCCACTAACGAGTCCTCCAAATGATTCAACTCTATGAATAAAGTTACAAAATAAAGGATATTAGTTACATACACAGGACTATATCTGTTATAGAAACAAACATGCTGACGATCCCCCTCAAGCTGATTAATAGGTATTTACTTTTCCCAGCTTGTATACAATATTCTGGAACATGGTCCTGCTTGGCCCTTTGGCAGTAATGTGGTCAACTAGTTGAAGGCCCCCTATTTTGCGGCCTTCGTGTAGAAGGCTTCCAATGCCTGTAAACAAAAAACAACATTTGGATTATAATGAAAAGATAAATTTAAATTTTGAACTTGATATCATTCAAACTTATGGTAGCAGTGAGGTACACTAAAAGGAATTTGCATTTGTAGGTTGACTTCATTATATCTCATATTTCTGTTGGCTGTAACAGGAGTATCATGAGTTGCAACACATCTTAATGGATAATTACCTTTTTAATGTATCAAACATCCACAAATAGAAAAGATAAAGAATAAATTCATATTCCTCTGGTCTTCTGATCTTGTAATGACGAGCACATGGATCATACAGCCATCTAATTTTCTGGCTGTCATGCATTTGAGTAATTCTTAGCATCATTACTGTTAAAGGCGTTTTGATTTCTTGTGATTCAGGTATTGTGAGGGGCATTGACACAGTTAAAGGCATGCTCTATCTGATTACACCTGTGCCATTTGGTTCTCTGAAAAAAGTCAACCTCTTGCTTCAGGGTAATATCCAAATTCCTACAAGTTTATTGCAGGTAAGAAATTGTTTTATGTACTTGCTCTTTCTTCTGAACATTAACTTGTAGAAATAATTCTTGTAAAAAAAGTTAACTTTATTACTTTGAGCTACCAAAGGAAAACTTTCTGTTGGTTTTTCCTTAGGTTACATGA
Protein sequence

>MS.gene08259.t1

MGSSGFVLPDIYIPQQWSQAANSITSSPTPPITFVCGASNCGKSTFSRNLLNVLLTRCDRVAYLETDVGQPEFTPPGFISLTIVNKVTPDLTIPCLKTPERSLFFGDVSSQKDPSTYLKYICSIYDYYHKEYCTFDKRQNSSKIHLPLVVNTSGWVKGDGYKVLVNMLKYIGPTHIVKIGISTDLKNLPGKFGSNGKNNRRVKLIEIYPARQDSLNKSVPAQKDARLVRDSRIIAYFRQCFPSDSDISTIKDLANSLTSHCPYNVPIASIKIRRVLHEVPISEILSSLNASIVGLAVESEGPKKLPWCLGLGIVRGIDTVKGMLYLITPVPFGSLKKVNLLLQGNIQIPTSLLQVT