AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene33828

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33828.t1 MTR_4g088595 100.000 248 0 0 1 248 1 248 0.0 501
MS.gene33828.t1 MTR_7g053160 96.774 248 8 0 1 248 1 248 4.65e-177 486
MS.gene33828.t1 MTR_2g014030 96.774 248 8 0 1 248 1 248 2.02e-176 485
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33828.t1 AT5G10360 91.968 249 18 1 1 247 1 249 7.41e-165 456
MS.gene33828.t1 AT4G31700 91.600 250 19 1 1 248 1 250 1.33e-157 437
MS.gene33828.t1 AT5G10360 73.896 249 11 2 1 247 1 197 4.09e-120 340
MS.gene33828.t1 AT4G31700 90.957 188 15 1 63 248 1 188 2.59e-109 313

Find 79 sgRNAs with CRISPR-Local

Find 173 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATATTGCAGCCGTGCTTTC+TGG 0.027256 4.4:+24244098 MS.gene33828:intron
TTGCTCTCTGCAGGAGTTTA+AGG 0.158668 4.4:+24244248 MS.gene33828:intron
GTTGAACAGCTTACGGATTT+TGG 0.198177 4.4:-24245094 None:intergenic
CAATTCTTGCTCGCTTCCTT+TGG 0.278075 4.4:-24245629 None:intergenic
GTGACCAATCGTTGTATCTT+TGG 0.296066 4.4:-24245594 None:intergenic
GGAAGTTCTTGGTGATGCTT+TGG 0.339745 4.4:+24244138 MS.gene33828:CDS
ATATTGCAGCCGTGCTTTCT+GGG 0.350681 4.4:+24244099 MS.gene33828:intron
TTCCCTCTGCTCCTTCAATC+TGG 0.366847 4.4:-24245718 None:intergenic
CTCTTAGGACCTCTCATCCT+TGG 0.367925 4.4:-24245066 None:intergenic
AAAGGTGATAATGATCTTCC+AGG 0.375447 4.4:+24245024 MS.gene33828:CDS
GACGCAATGGAGAGCGTAGA+AGG 0.381140 4.4:+24244938 MS.gene33828:CDS
GTCAACACTCCCTGCTTCAT+TGG 0.384872 4.4:-24244315 None:intergenic
TGTTGACCCCTGGCCGTGTT+AGG 0.390290 4.4:+24244331 MS.gene33828:CDS
TGCTCTCTGCAGGAGTTTAA+GGG 0.392051 4.4:+24244249 MS.gene33828:intron
AGGATCTCACAGGAAGTTCT+TGG 0.420484 4.4:+24244127 MS.gene33828:CDS
GAAGTTCTTGGTGATGCTTT+GGG 0.420593 4.4:+24244139 MS.gene33828:CDS
ACTCCTTGCTTCCAGATTGA+AGG 0.422012 4.4:+24245707 MS.gene33828:CDS
TGGCTTAGCACTGGATAGTC+TGG 0.428627 4.4:-24245772 None:intergenic
TTCTTGCTCGCTTCCTTTGG+AGG 0.436168 4.4:-24245626 None:intergenic
CGCTTCCTTTGGAGGGTCAA+AGG 0.448439 4.4:-24245618 None:intergenic
TCAACACTCCCTGCTTCATT+GGG 0.455023 4.4:-24244314 None:intergenic
CTGACATCCAGTGGTTGGAT+TGG 0.459762 4.4:-24243409 None:intergenic
TTGTTTGATTGCAGGGAAGA+AGG 0.460053 4.4:+24245560 MS.gene33828:intron
TTGACAGGTTGAACAGCTTA+CGG 0.462619 4.4:-24245101 None:intergenic
AGCAAGAGCCTAACACGGCC+AGG 0.463109 4.4:-24244339 None:intergenic
ACAAGGATTCCCAATGAAGC+AGG 0.478682 4.4:+24244305 MS.gene33828:CDS
TTCTTCTGACATCCAGTGGT+TGG 0.481727 4.4:-24243414 None:intergenic
AAGTTCAAAACAGAGAGGTC+GGG 0.481755 4.4:-24244988 None:intergenic
GCTGCTTATGCTGTGGCAGA+TGG 0.483327 4.4:-24245792 None:intergenic
GCACATCATCATCCTTTGAC+AGG 0.490483 4.4:-24245116 None:intergenic
AACTTGGTTATTGTGAAGAA+AGG 0.498754 4.4:+24245006 MS.gene33828:CDS
AATCAGAAACTTACCATCCT+TGG 0.507683 4.4:-24245175 None:intergenic
AACGTTGCCAATCCAACCAC+TGG 0.507932 4.4:+24243402 MS.gene33828:CDS
TCTTGCTCGCTTCCTTTGGA+GGG 0.509236 4.4:-24245625 None:intergenic
GCAAGAGCCTAACACGGCCA+GGG 0.526221 4.4:-24244338 None:intergenic
GGTTATGTCTTCAAGATCAC+TGG 0.529877 4.4:+24244270 MS.gene33828:CDS
AAGCTTCTTCTGACATCCAG+TGG 0.530433 4.4:-24243418 None:intergenic
TATGTCTTCAAGATCACTGG+TGG 0.541337 4.4:+24244273 MS.gene33828:CDS
AAGCAGGGAGTGTTGACCCC+TGG 0.544754 4.4:+24244321 MS.gene33828:CDS
TTCTCAACATCAGTAAGCCC+TGG 0.548857 4.4:-24245042 None:intergenic
GCCGTGCTTTCTGGGACAAG+AGG 0.553103 4.4:+24244107 MS.gene33828:intron
CAAGTTCAAAACAGAGAGGT+CGG 0.553854 4.4:-24244989 None:intergenic
GGAACGCCGAAGTGAGAGCT+TGG 0.554466 4.4:+24245737 MS.gene33828:CDS
CTTCCGTGTCCACGGAAACA+AGG 0.556351 4.4:-24244916 None:intergenic
GCAGGAACTCCTTGTTTCCG+TGG 0.557291 4.4:+24244907 MS.gene33828:intron
CAGAAACTTACCATCCTTGG+TGG 0.557776 4.4:-24245172 None:intergenic
CAAGAAGAAGAGAATTGCTA+AGG 0.559648 4.4:+24245656 MS.gene33828:CDS
TCCTCTTGTCCCAGAAAGCA+CGG 0.564472 4.4:-24244108 None:intergenic
ACTCCTTGTTTCCGTGGACA+CGG 0.569669 4.4:+24244913 MS.gene33828:CDS
AGCTCCAAAGATACAACGAT+TGG 0.570130 4.4:+24245590 MS.gene33828:CDS
TCACTCCTTTGACCCTCCAA+AGG 0.572684 4.4:+24245613 MS.gene33828:CDS
CGTAGAAGGAAGTCTGTTCG+TGG 0.573391 4.4:+24244952 MS.gene33828:CDS
GCTCCTTCAATCTGGAAGCA+AGG 0.577913 4.4:-24245710 None:intergenic
CCTTGGTGGTGAAAGTTCTA+CGG 0.579080 4.4:-24245158 None:intergenic
TTCCAGATTGAAGGAGCAGA+GGG 0.584168 4.4:+24245716 MS.gene33828:CDS
AGAACTTTCACCACCAAGGA+TGG 0.589936 4.4:+24245162 MS.gene33828:CDS
CATTGCGTCTTCCGTGTCCA+CGG 0.591891 4.4:-24244924 None:intergenic
CTGGGACAAGAGGATCTCAC+AGG 0.592723 4.4:+24244117 MS.gene33828:CDS
TAAGCTGTTCAACCTGTCAA+AGG 0.594543 4.4:+24245104 MS.gene33828:CDS
AGTTCAAAACAGAGAGGTCG+GGG 0.595017 4.4:-24244987 None:intergenic
CGTGGACACGGAAGACGCAA+TGG 0.595810 4.4:+24244925 MS.gene33828:CDS
TTACTGATGTTGAGAAGCCA+AGG 0.596042 4.4:+24245049 MS.gene33828:CDS
GTTGAGAAGCCAAGGATGAG+AGG 0.601224 4.4:+24245057 MS.gene33828:CDS
TGTGGCAGATGGCTTAGCAC+TGG 0.603451 4.4:-24245781 None:intergenic
AAGGTGATAATGATCTTCCA+GGG 0.607328 4.4:+24245025 MS.gene33828:CDS
GTTAGGCTCTTGCTCCATAG+AGG 0.608724 4.4:+24244348 MS.gene33828:CDS
TCTTAGCCAAGCTCTCACTT+CGG 0.610174 4.4:-24245743 None:intergenic
GTGAGAGCTTGGCTAAGAAG+AGG 0.611225 4.4:+24245748 MS.gene33828:CDS
CAAGAGCCTAACACGGCCAG+GGG 0.618346 4.4:-24244337 None:intergenic
CTTCCAGATTGAAGGAGCAG+AGG 0.620391 4.4:+24245715 MS.gene33828:CDS
ATGTCTTCAAGATCACTGGT+GGG 0.622519 4.4:+24244274 MS.gene33828:CDS
AATTGCTAAGGCAAAATCAG+AGG 0.627856 4.4:+24245668 MS.gene33828:CDS
GGTGGTGAAAGTTCTACGGT+AGG 0.627958 4.4:-24245154 None:intergenic
CAAGGATTCCCAATGAAGCA+GGG 0.635106 4.4:+24244306 MS.gene33828:CDS
ACTGGTGGGTGTGACAAACA+AGG 0.636404 4.4:+24244288 MS.gene33828:CDS
GCTGTAAGCTGCTTATGCTG+TGG 0.655143 4.4:-24245799 None:intergenic
CCGTAGAACTTTCACCACCA+AGG 0.691015 4.4:+24245158 MS.gene33828:CDS
TATGGAGCAAGAGCCTAACA+CGG 0.710814 4.4:-24244344 None:intergenic
TAACCAAGTTCAAAACAGAG+AGG 0.738467 4.4:-24244993 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATATATCCTTATATTAA+GGG + chr4.4:24245220-24245239 MS.gene33828:intron 10.0%
!! CTTTATATATCCTTATATTA+AGG + chr4.4:24245219-24245238 MS.gene33828:intron 15.0%
!! TATAAGGATATATAAAGAAA+TGG - chr4.4:24245216-24245235 None:intergenic 15.0%
!! AAGAATATTACTTTCATTGA+AGG + chr4.4:24245380-24245399 MS.gene33828:intron 20.0%
!! ACATGAGATAAATAGTAAAA+TGG - chr4.4:24244516-24244535 None:intergenic 20.0%
!! CAGAAAATTGAAAAGAATTT+CGG - chr4.4:24243596-24243615 None:intergenic 20.0%
!! TAAATAGATTGAAGATTTGA+AGG - chr4.4:24244388-24244407 None:intergenic 20.0%
!! TAGCTTATGAAAATAGTTTA+TGG + chr4.4:24243850-24243869 MS.gene33828:intron 20.0%
!!! TCATTTGAAGTATTTTTCTT+AGG + chr4.4:24243502-24243521 MS.gene33828:intron 20.0%
!!! TTAAGTTGTTTATCAAAACA+AGG + chr4.4:24243983-24244002 MS.gene33828:intron 20.0%
!!! TTTTTGTGAAGTTATTTATG+TGG + chr4.4:24244046-24244065 MS.gene33828:intron 20.0%
! AAAAAAAAATGCTACCTCTA+TGG - chr4.4:24244365-24244384 None:intergenic 25.0%
! AACAATAATCATTGTGATCA+TGG - chr4.4:24244784-24244803 None:intergenic 25.0%
! ACATCCAAAACTATTTAACT+AGG - chr4.4:24244678-24244697 None:intergenic 25.0%
! AGAACAAAAATGAATACAAG+TGG - chr4.4:24245322-24245341 None:intergenic 25.0%
! CGTTCATAAATTTATAGTAG+TGG + chr4.4:24244467-24244486 MS.gene33828:intron 25.0%
! CTTAGTATTTCATTATTTGC+AGG + chr4.4:24244843-24244862 MS.gene33828:intron 25.0%
! GCAAATAATGAAATACTAAG+TGG - chr4.4:24244843-24244862 None:intergenic 25.0%
! GTAATGAATTGTAATCTTAG+TGG + chr4.4:24245534-24245553 MS.gene33828:intron 25.0%
! GTTATCTCTAATATTACATG+TGG - chr4.4:24244598-24244617 None:intergenic 25.0%
! TCATAAATTTATAGTAGTGG+AGG + chr4.4:24244470-24244489 MS.gene33828:intron 25.0%
! TCTTCAATCTATTTACCTTT+CGG + chr4.4:24244393-24244412 MS.gene33828:intron 25.0%
! TTACAATGCTCTACAAAATA+AGG - chr4.4:24244205-24244224 None:intergenic 25.0%
! TTCATTACAAATGTCTGTAT+TGG - chr4.4:24245522-24245541 None:intergenic 25.0%
!! ACTATTTTCATAAGCTATCT+TGG - chr4.4:24243847-24243866 None:intergenic 25.0%
!! AGTTCTTTTCATAAAGTTAG+TGG - chr4.4:24244646-24244665 None:intergenic 25.0%
!! CATTGTCATTTATCTGAATT+TGG + chr4.4:24243710-24243729 MS.gene33828:intron 25.0%
!!! ACAATATGTTTTGATATGCA+TGG + chr4.4:24243678-24243697 MS.gene33828:intron 25.0%
!!! ATTTGGTTTGAACTGATATT+TGG + chr4.4:24243727-24243746 MS.gene33828:intron 25.0%
!!! TAATTTTTTATGCACTTTGC+AGG + chr4.4:24244889-24244908 MS.gene33828:intron 25.0%
!!! TTAAATAGTTTTGGATGTGT+GGG + chr4.4:24244680-24244699 MS.gene33828:intron 25.0%
!!! TTTATTTTATCGTGTGTAGT+AGG + chr4.4:24243895-24243914 MS.gene33828:intron 25.0%
AACTTGGTTATTGTGAAGAA+AGG + chr4.4:24245006-24245025 MS.gene33828:CDS 30.0%
ACAAAAATGAATACAAGTGG+AGG - chr4.4:24245319-24245338 None:intergenic 30.0%
ACAGTATACATTGTCTAGAT+TGG + chr4.4:24245415-24245434 MS.gene33828:intron 30.0%
AGAAATGGTTACAATATTCG+TGG - chr4.4:24245201-24245220 None:intergenic 30.0%
AGTATACATTGTCTAGATTG+GGG + chr4.4:24245417-24245436 MS.gene33828:intron 30.0%
ATATGACTGTAGTGTAAATC+TGG + chr4.4:24243646-24243665 MS.gene33828:intron 30.0%
ATCCTTATATTAAGGGTAGT+GGG + chr4.4:24245227-24245246 MS.gene33828:intron 30.0%
CAGTATACATTGTCTAGATT+GGG + chr4.4:24245416-24245435 MS.gene33828:intron 30.0%
CCAAAACTATTTAACTAGGA+GGG - chr4.4:24244674-24244693 None:intergenic 30.0%
CCAACATAGATTTATGAACA+TGG - chr4.4:24243552-24243571 None:intergenic 30.0%
CCATGTTCATAAATCTATGT+TGG + chr4.4:24243549-24243568 MS.gene33828:intron 30.0%
GAAAATGAAGAGAGAGTAAA+TGG - chr4.4:24243475-24243494 None:intergenic 30.0%
GTGAACTTGTAGAAATAAGT+TGG - chr4.4:24243825-24243844 None:intergenic 30.0%
TATCCTTATATTAAGGGTAG+TGG + chr4.4:24245226-24245245 MS.gene33828:intron 30.0%
TCCAAAACTATTTAACTAGG+AGG - chr4.4:24244675-24244694 None:intergenic 30.0%
TCTCCAATAGTAATACTAAG+AGG - chr4.4:24244447-24244466 None:intergenic 30.0%
TTGCCTCTTAGTATTACTAT+TGG + chr4.4:24244441-24244460 MS.gene33828:intron 30.0%
! GCATATGTAGTTATTTCAGT+AGG + chr4.4:24244564-24244583 MS.gene33828:intron 30.0%
! GTGATTTTGCATTGGATTAT+TGG + chr4.4:24244534-24244553 MS.gene33828:intron 30.0%
! TATCTCATGTGATTTTGCAT+TGG + chr4.4:24244526-24244545 MS.gene33828:intron 30.0%
! TGTAGAAATAAGTTGGAAAC+AGG - chr4.4:24243818-24243837 None:intergenic 30.0%
!! CTGTATTGGTACAATTTTAC+CGG - chr4.4:24245508-24245527 None:intergenic 30.0%
!!! GTTAAATAGTTTTGGATGTG+TGG + chr4.4:24244679-24244698 MS.gene33828:intron 30.0%
AAAAAATCCATGCCATAGTG+AGG + chr4.4:24244724-24244743 MS.gene33828:intron 35.0%
AAAAATCCATGCCATAGTGA+GGG + chr4.4:24244725-24244744 MS.gene33828:intron 35.0%
AAAACGGCACTTCTTTCAAA+CGG - chr4.4:24245453-24245472 None:intergenic 35.0%
AAACAGTTGTCCAAACTACT+AGG + chr4.4:24245283-24245302 MS.gene33828:intron 35.0%
AACAGTTGTCCAAACTACTA+GGG + chr4.4:24245284-24245303 MS.gene33828:intron 35.0%
AATCAGAAACTTACCATCCT+TGG - chr4.4:24245178-24245197 None:intergenic 35.0%
AATTGCTAAGGCAAAATCAG+AGG + chr4.4:24245668-24245687 MS.gene33828:CDS 35.0%
ATCGCAAATTGTGAGGAAAA+CGG - chr4.4:24245469-24245488 None:intergenic 35.0%
CAAGAAGAAGAGAATTGCTA+AGG + chr4.4:24245656-24245675 MS.gene33828:CDS 35.0%
CTAAACAATCCAATCCAAAC+AGG - chr4.4:24243755-24243774 None:intergenic 35.0%
TAACCAAGTTCAAAACAGAG+AGG - chr4.4:24244996-24245015 None:intergenic 35.0%
TCCTTATATTAAGGGTAGTG+GGG + chr4.4:24245228-24245247 MS.gene33828:intron 35.0%
TGGGAAACATGCATATAGTA+TGG + chr4.4:24244699-24244718 MS.gene33828:intron 35.0%
! AAAGGTGATAATGATCTTCC+AGG + chr4.4:24245024-24245043 MS.gene33828:CDS 35.0%
! AAGGTGATAATGATCTTCCA+GGG + chr4.4:24245025-24245044 MS.gene33828:CDS 35.0%
! CCCTCCTAGTTAAATAGTTT+TGG + chr4.4:24244671-24244690 MS.gene33828:intron 35.0%
! GAGTAAGCTTCTTTTCTACT+TGG + chr4.4:24244865-24244884 MS.gene33828:intron 35.0%
AAACGGCACTTCTTTCAAAC+GGG - chr4.4:24245452-24245471 None:intergenic 40.0%
AAGGACAATCGCAAATTGTG+AGG - chr4.4:24245476-24245495 None:intergenic 40.0%
AAGTTCAAAACAGAGAGGTC+GGG - chr4.4:24244991-24245010 None:intergenic 40.0%
ACAGTTGTCCAAACTACTAG+GGG + chr4.4:24245285-24245304 MS.gene33828:intron 40.0%
AGCTCCAAAGATACAACGAT+TGG + chr4.4:24245590-24245609 MS.gene33828:CDS 40.0%
ATGTCTTCAAGATCACTGGT+GGG + chr4.4:24244274-24244293 MS.gene33828:CDS 40.0%
CAAGTTCAAAACAGAGAGGT+CGG - chr4.4:24244992-24245011 None:intergenic 40.0%
GGTTATGTCTTCAAGATCAC+TGG + chr4.4:24244270-24244289 MS.gene33828:CDS 40.0%
GTGACCAATCGTTGTATCTT+TGG - chr4.4:24245597-24245616 None:intergenic 40.0%
TAAAGTTAGTGGAGACGTTG+AGG - chr4.4:24244635-24244654 None:intergenic 40.0%
TAAGCTGTTCAACCTGTCAA+AGG + chr4.4:24245104-24245123 MS.gene33828:CDS 40.0%
TATGTCTTCAAGATCACTGG+TGG + chr4.4:24244273-24244292 MS.gene33828:CDS 40.0%
TTACTGATGTTGAGAAGCCA+AGG + chr4.4:24245049-24245068 MS.gene33828:CDS 40.0%
TTGTTTGATTGCAGGGAAGA+AGG + chr4.4:24245560-24245579 MS.gene33828:intron 40.0%
! ACTGATATTTGGAGCCTGTT+TGG + chr4.4:24243738-24243757 MS.gene33828:intron 40.0%
! ATTTTACCGGATGTCACTGA+AGG - chr4.4:24245495-24245514 None:intergenic 40.0%
! CCTTATATTAAGGGTAGTGG+GGG + chr4.4:24245229-24245248 MS.gene33828:intron 40.0%
! GTTGAACAGCTTACGGATTT+TGG - chr4.4:24245097-24245116 None:intergenic 40.0%
! TATTTGGAGCCTGTTTGGAT+TGG + chr4.4:24243743-24243762 MS.gene33828:intron 40.0%
! TTGACAGGTTGAACAGCTTA+CGG - chr4.4:24245104-24245123 None:intergenic 40.0%
!! AGTGGTTGTTGTTTGATTGC+AGG + chr4.4:24245552-24245571 MS.gene33828:intron 40.0%
!! GAAGTTCTTGGTGATGCTTT+GGG + chr4.4:24244139-24244158 MS.gene33828:CDS 40.0%
!! GTGGTTGTTGTTTGATTGCA+GGG + chr4.4:24245553-24245572 MS.gene33828:intron 40.0%
AAGCTTCTTCTGACATCCAG+TGG - chr4.4:24243421-24243440 None:intergenic 45.0%
ACAAGGATTCCCAATGAAGC+AGG + chr4.4:24244305-24244324 MS.gene33828:CDS 45.0%
ACTCCTTGCTTCCAGATTGA+AGG + chr4.4:24245707-24245726 MS.gene33828:CDS 45.0%
AGAACTTTCACCACCAAGGA+TGG + chr4.4:24245162-24245181 MS.gene33828:CDS 45.0%
AGGATCTCACAGGAAGTTCT+TGG + chr4.4:24244127-24244146 MS.gene33828:CDS 45.0%
AGTTCAAAACAGAGAGGTCG+GGG - chr4.4:24244990-24245009 None:intergenic 45.0%
ATATTGCAGCCGTGCTTTCT+GGG + chr4.4:24244099-24244118 MS.gene33828:intron 45.0%
CAAGGATTCCCAATGAAGCA+GGG + chr4.4:24244306-24244325 MS.gene33828:CDS 45.0%
CAATTCTTGCTCGCTTCCTT+TGG - chr4.4:24245632-24245651 None:intergenic 45.0%
CAGAAACTTACCATCCTTGG+TGG - chr4.4:24245175-24245194 None:intergenic 45.0%
CCCCCACTACCCTTAATATA+AGG - chr4.4:24245232-24245251 None:intergenic 45.0%
CTATGAAGCCCCTAGTAGTT+TGG - chr4.4:24245296-24245315 None:intergenic 45.0%
GATTGTCCTTCAGTGACATC+CGG + chr4.4:24245486-24245505 MS.gene33828:intron 45.0%
GCAACATACAGTATGCCGAA+AGG - chr4.4:24244411-24244430 None:intergenic 45.0%
GCACATCATCATCCTTTGAC+AGG - chr4.4:24245119-24245138 None:intergenic 45.0%
TATATTGCAGCCGTGCTTTC+TGG + chr4.4:24244098-24244117 MS.gene33828:intron 45.0%
TATGGAGCAAGAGCCTAACA+CGG - chr4.4:24244347-24244366 None:intergenic 45.0%
TCAACACTCCCTGCTTCATT+GGG - chr4.4:24244317-24244336 None:intergenic 45.0%
TCTTAGCCAAGCTCTCACTT+CGG - chr4.4:24245746-24245765 None:intergenic 45.0%
TGCTCTCTGCAGGAGTTTAA+GGG + chr4.4:24244249-24244268 MS.gene33828:intron 45.0%
TTCCAGATTGAAGGAGCAGA+GGG + chr4.4:24245716-24245735 MS.gene33828:CDS 45.0%
TTCTCAACATCAGTAAGCCC+TGG - chr4.4:24245045-24245064 None:intergenic 45.0%
TTCTTCTGACATCCAGTGGT+TGG - chr4.4:24243417-24243436 None:intergenic 45.0%
TTGCTCTCTGCAGGAGTTTA+AGG + chr4.4:24244248-24244267 MS.gene33828:intron 45.0%
! CGGATTTTGGATGCTCTCTT+AGG - chr4.4:24245084-24245103 None:intergenic 45.0%
! GTTGTGATTTTGCTCTCTGC+AGG + chr4.4:24244239-24244258 MS.gene33828:intron 45.0%
! TGGAGACGTTGAGGTATTTG+TGG - chr4.4:24244626-24244645 None:intergenic 45.0%
!! CCTTGGTGGTGAAAGTTCTA+CGG - chr4.4:24245161-24245180 None:intergenic 45.0%
!! CGACCTCTCTGTTTTGAACT+TGG + chr4.4:24244990-24245009 MS.gene33828:CDS 45.0%
!! GGAAGTTCTTGGTGATGCTT+TGG + chr4.4:24244138-24244157 MS.gene33828:CDS 45.0%
AACGTTGCCAATCCAACCAC+TGG + chr4.4:24243402-24243421 MS.gene33828:CDS 50.0%
ATCATGGACAGCCCTACCTT+AGG - chr4.4:24244768-24244787 None:intergenic 50.0%
CCGTAGAACTTTCACCACCA+AGG + chr4.4:24245158-24245177 MS.gene33828:CDS 50.0%
CGTAGAAGGAAGTCTGTTCG+TGG + chr4.4:24244952-24244971 MS.gene33828:CDS 50.0%
CTCTTAGGACCTCTCATCCT+TGG - chr4.4:24245069-24245088 None:intergenic 50.0%
CTGACATCCAGTGGTTGGAT+TGG - chr4.4:24243412-24243431 None:intergenic 50.0%
CTTCCAGATTGAAGGAGCAG+AGG + chr4.4:24245715-24245734 MS.gene33828:CDS 50.0%
GCTCCTTCAATCTGGAAGCA+AGG - chr4.4:24245713-24245732 None:intergenic 50.0%
GGTGGTGAAAGTTCTACGGT+AGG - chr4.4:24245157-24245176 None:intergenic 50.0%
GTCAACACTCCCTGCTTCAT+TGG - chr4.4:24244318-24244337 None:intergenic 50.0%
GTGAGAGCTTGGCTAAGAAG+AGG + chr4.4:24245748-24245767 MS.gene33828:CDS 50.0%
GTTAGGCTCTTGCTCCATAG+AGG + chr4.4:24244348-24244367 MS.gene33828:CDS 50.0%
GTTGAGAAGCCAAGGATGAG+AGG + chr4.4:24245057-24245076 MS.gene33828:CDS 50.0%
TCACTCCTTTGACCCTCCAA+AGG + chr4.4:24245613-24245632 MS.gene33828:CDS 50.0%
TCATGGACAGCCCTACCTTA+GGG - chr4.4:24244767-24244786 None:intergenic 50.0%
TCCTCTTGTCCCAGAAAGCA+CGG - chr4.4:24244111-24244130 None:intergenic 50.0%
TCTTGCTCGCTTCCTTTGGA+GGG - chr4.4:24245628-24245647 None:intergenic 50.0%
TTCCCTCTGCTCCTTCAATC+TGG - chr4.4:24245721-24245740 None:intergenic 50.0%
TTCTTGCTCGCTTCCTTTGG+AGG - chr4.4:24245629-24245648 None:intergenic 50.0%
! ACTCCTTGTTTCCGTGGACA+CGG + chr4.4:24244913-24244932 MS.gene33828:CDS 50.0%
! ACTGGTGGGTGTGACAAACA+AGG + chr4.4:24244288-24244307 MS.gene33828:CDS 50.0%
! TGGCTTAGCACTGGATAGTC+TGG - chr4.4:24245775-24245794 None:intergenic 50.0%
CATGGACAGCCCTACCTTAG+GGG - chr4.4:24244766-24244785 None:intergenic 55.0%
CATTGCGTCTTCCGTGTCCA+CGG - chr4.4:24244927-24244946 None:intergenic 55.0%
CTGGGACAAGAGGATCTCAC+AGG + chr4.4:24244117-24244136 MS.gene33828:CDS 55.0%
CTTCCGTGTCCACGGAAACA+AGG - chr4.4:24244919-24244938 None:intergenic 55.0%
TGTGGCAGATGGCTTAGCAC+TGG - chr4.4:24245784-24245803 None:intergenic 55.0%
! CGCTTCCTTTGGAGGGTCAA+AGG - chr4.4:24245621-24245640 None:intergenic 55.0%
! GACGCAATGGAGAGCGTAGA+AGG + chr4.4:24244938-24244957 MS.gene33828:CDS 55.0%
! GCAGGAACTCCTTGTTTCCG+TGG + chr4.4:24244907-24244926 MS.gene33828:intron 55.0%
AGCAAGAGCCTAACACGGCC+AGG - chr4.4:24244342-24244361 None:intergenic 60.0%
CAAGAGCCTAACACGGCCAG+GGG - chr4.4:24244340-24244359 None:intergenic 60.0%
CCATGCCATAGTGAGGGCCA+AGG + chr4.4:24244731-24244750 MS.gene33828:intron 60.0%
CCCACACCTCCCCTAAGGTA+GGG + chr4.4:24244754-24244773 MS.gene33828:intron 60.0%
CCTTGGCCCTCACTATGGCA+TGG - chr4.4:24244734-24244753 None:intergenic 60.0%
CGTGGACACGGAAGACGCAA+TGG + chr4.4:24244925-24244944 MS.gene33828:CDS 60.0%
GCAAGAGCCTAACACGGCCA+GGG - chr4.4:24244341-24244360 None:intergenic 60.0%
GGAACGCCGAAGTGAGAGCT+TGG + chr4.4:24245737-24245756 MS.gene33828:CDS 60.0%
TGTTGACCCCTGGCCGTGTT+AGG + chr4.4:24244331-24244350 MS.gene33828:CDS 60.0%
! CCCTACCTTAGGGGAGGTGT+GGG - chr4.4:24244757-24244776 None:intergenic 60.0%
!! AAGCAGGGAGTGTTGACCCC+TGG + chr4.4:24244321-24244340 MS.gene33828:CDS 60.0%
!! GCCGTGCTTTCTGGGACAAG+AGG + chr4.4:24244107-24244126 MS.gene33828:intron 60.0%
CAAGGCCCACACCTCCCCTA+AGG + chr4.4:24244749-24244768 MS.gene33828:intron 65.0%
GCCCACACCTCCCCTAAGGT+AGG + chr4.4:24244753-24244772 MS.gene33828:intron 65.0%
GCCCTACCTTAGGGGAGGTG+TGG - chr4.4:24244758-24244777 None:intergenic 65.0%
GGACAGCCCTACCTTAGGGG+AGG - chr4.4:24244763-24244782 None:intergenic 65.0%
GTGGGCCTTGGCCCTCACTA+TGG - chr4.4:24244739-24244758 None:intergenic 65.0%
! CTTAGGGGAGGTGTGGGCCT+TGG - chr4.4:24244751-24244770 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 24243300 24245809 24243300 ID=MS.gene33828
chr4.4 mRNA 24243300 24245809 24243300 ID=MS.gene33828.t1;Parent=MS.gene33828
chr4.4 exon 24243300 24243305 24243300 ID=MS.gene33828.t1.exon1;Parent=MS.gene33828.t1
chr4.4 CDS 24243300 24243305 24243300 ID=cds.MS.gene33828.t1;Parent=MS.gene33828.t1
chr4.4 exon 24243399 24243463 24243399 ID=MS.gene33828.t1.exon2;Parent=MS.gene33828.t1
chr4.4 CDS 24243399 24243463 24243399 ID=cds.MS.gene33828.t1;Parent=MS.gene33828.t1
chr4.4 exon 24244108 24244165 24244108 ID=MS.gene33828.t1.exon3;Parent=MS.gene33828.t1
chr4.4 CDS 24244108 24244165 24244108 ID=cds.MS.gene33828.t1;Parent=MS.gene33828.t1
chr4.4 exon 24244261 24244369 24244261 ID=MS.gene33828.t1.exon4;Parent=MS.gene33828.t1
chr4.4 CDS 24244261 24244369 24244261 ID=cds.MS.gene33828.t1;Parent=MS.gene33828.t1
chr4.4 exon 24244911 24245183 24244911 ID=MS.gene33828.t1.exon5;Parent=MS.gene33828.t1
chr4.4 CDS 24244911 24245183 24244911 ID=cds.MS.gene33828.t1;Parent=MS.gene33828.t1
chr4.4 exon 24245574 24245809 24245574 ID=MS.gene33828.t1.exon6;Parent=MS.gene33828.t1
chr4.4 CDS 24245574 24245809 24245574 ID=cds.MS.gene33828.t1;Parent=MS.gene33828.t1
Gene Sequence

>MS.gene33828

ATGAAGGTTTGTTTTCAATCTCTTCAATTCTTCGATTTCAATGTTGTTGTTGTTGTTGTTTCATTAAACTGAATCATTGTGTTTTCGTTTTTGTTTCAGTTTAACGTTGCCAATCCAACCACTGGATGTCAGAAGAAGCTTGAAATCGATGATGACATGAAGCTGTAATAATCCATTTACTCTCTCTTCATTTTCGCATTTTTCATTTGAAGTATTTTTCTTAGGACAAAAATCATTACTTTGAAGTGACCATGTTCATAAATCTATGTTGGTTTCTGTTCTTTATGTTTGTGCCGAAATTCTTTTCAATTTTCTGTGTAATTGTTTTTTTTTTCTTTTCATTTTTATATGACTGTAGTGTAAATCTGGTACTTGTTTACAATATGTTTTGATATGCATGGTGAAATGAACATTGTCATTTATCTGAATTTGGTTTGAACTGATATTTGGAGCCTGTTTGGATTGGATTGTTTAGTCGAGAGAATTTATAAAAACAACTTATGACATGTTTATAACCTGTTTCCAACTTATTTCTACAAGTTCACCAAGATAGCTTATGAAAATAGTTTATGGCTTAATACAAAAACAATCTGACTTTATTTTATCGTGTGTAGTAGGAATAGTTTATTCACAAGTGCTTGTTGTTAGTGCTTATTATAAGCACTTGTGCTATATGCTGCAAATTAAGTTGTTTATCAAAACAAGGTCTCAGTGTAGTGCTATTGATAGTTTCTTTTGTATGTTTGTTTTTGTGAAGTTATTTATGTGGCTTCTCTTTGATTATTTAATTATCTTTTGTATATTGCAGCCGTGCTTTCTGGGACAAGAGGATCTCACAGGAAGTTCTTGGTGATGCTTTGGGAGAAGTATGATCTCTAGCATGAAATTTGCGTTGTCTGCTTCCTTATTTTGTAGAGCATTGTAATTGATCTATTTTGTGTTGTGATTTTGCTCTCTGCAGGAGTTTAAGGGTTATGTCTTCAAGATCACTGGTGGGTGTGACAAACAAGGATTCCCAATGAAGCAGGGAGTGTTGACCCCTGGCCGTGTTAGGCTCTTGCTCCATAGAGGTAGCATTTTTTTTTCCTTCAAATCTTCAATCTATTTACCTTTCGGCATACTGTATGTTGCATTGAAAATTTTGCCTCTTAGTATTACTATTGGAGACGTTCATAAATTTATAGTAGTGGAGGCATTATAAGTAAAGAAACAACCATTTTACTATTTATCTCATGTGATTTTGCATTGGATTATTGGTAATATAGCATATGTAGTTATTTCAGTAGGTGCAATTTCCACATGTAATATTAGAGATAACTTTAACCACAAATACCTCAACGTCTCCACTAACTTTATGAAAAGAACTTTATTCCCTCCTAGTTAAATAGTTTTGGATGTGTGGGAAACATGCATATAGTATGGAAAAAAAATCCATGCCATAGTGAGGGCCAAGGCCCACACCTCCCCTAAGGTAGGGCTGTCCATGATCACAATGATTATTGTTTGTGATGCAACACGTAATGTTTTATGTTTTTGTGCTCCACTTAGTATTTCATTATTTGCAGGAGTAAGCTTCTTTTCTACTTGGCTAATTTTTTATGCACTTTGCAGGAACTCCTTGTTTCCGTGGACACGGAAGACGCAATGGAGAGCGTAGAAGGAAGTCTGTTCGTGGATGCATTGTCAGCCCCGACCTCTCTGTTTTGAACTTGGTTATTGTGAAGAAAGGTGATAATGATCTTCCAGGGCTTACTGATGTTGAGAAGCCAAGGATGAGAGGTCCTAAGAGAGCATCCAAAATCCGTAAGCTGTTCAACCTGTCAAAGGATGATGATGTGCGAAAATATGTTAACACCTACCGTAGAACTTTCACCACCAAGGATGGTAAGTTTCTGATTCCACGAATATTGTAACCATTTCTTTATATATCCTTATATTAAGGGTAGTGGGGGAGATGTAGAATTAAAATTTTTTTTGATTAAAAAACAGTTGTCCAAACTACTAGGGGCTTCATAGCCTCCACTTGTATTCATTTTTGTTCTAGCAGAGACTTGCTGTAGTTTTCTTTGTTTTAAGTATAAAGAATATTACTTTCATTGAAGGCAGTGATACTTTACAGTATACATTGTCTAGATTGGGGTTTAGAGTACCCGTTTGAAAGAAGTGCCGTTTTCCTCACAATTTGCGATTGTCCTTCAGTGACATCCGGTAAAATTGTACCAATACAGACATTTGTAATGAATTGTAATCTTAGTGGTTGTTGTTTGATTGCAGGGAAGAAGGTTAGCAAAGCTCCAAAGATACAACGATTGGTCACTCCTTTGACCCTCCAAAGGAAGCGAGCAAGAATTGCAGACAAGAAGAAGAGAATTGCTAAGGCAAAATCAGAGGCAGCAGATTATCAGAAACTCCTTGCTTCCAGATTGAAGGAGCAGAGGGAACGCCGAAGTGAGAGCTTGGCTAAGAAGAGGTCCAGACTATCCAGTGCTAAGCCATCTGCCACAGCATAA
Protein sequence

>MS.gene33828.t1

MKFNVANPTTGCQKKLEIDDDMKLRAFWDKRISQEVLGDALGEEFKGYVFKITGGCDKQGFPMKQGVLTPGRVRLLLHRGTPCFRGHGRRNGERRRKSVRGCIVSPDLSVLNLVIVKKGDNDLPGLTDVEKPRMRGPKRASKIRKLFNLSKDDDVRKYVNTYRRTFTTKDGKKVSKAPKIQRLVTPLTLQRKRARIADKKKRIAKAKSEAADYQKLLASRLKEQRERRSESLAKKRSRLSSAKPSATA