Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00279.t1 | XP_013463211.1 | 97.9 | 188 | 4 | 0 | 1 | 188 | 1 | 188 | 4.80E-93 | 350.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00279.t1 | Q84WN3 | 60.1 | 188 | 74 | 1 | 1 | 187 | 1 | 188 | 3.3e-58 | 226.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00279.t1 | A0A072VGC9 | 97.9 | 188 | 4 | 0 | 1 | 188 | 1 | 188 | 3.5e-93 | 350.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene00279 | MS.gene02493 | PPI |
| MS.gene00279 | MS.gene026679 | PPI |
| MS.gene00279 | MS.gene93151 | PPI |
| MS.gene90281 | MS.gene00279 | PPI |
| MS.gene69746 | MS.gene00279 | PPI |
| MS.gene00279 | MS.gene004537 | PPI |
| MS.gene00279 | MS.gene009329 | PPI |
| MS.gene00279 | MS.gene31850 | PPI |
| MS.gene00279 | MS.gene044269 | PPI |
| MS.gene013686 | MS.gene00279 | PPI |
| MS.gene68896 | MS.gene00279 | PPI |
| MS.gene00279 | MS.gene70529 | PPI |
| MS.gene00279 | MS.gene81949 | PPI |
| MS.gene00279 | MS.gene46188 | PPI |
| MS.gene00279 | MS.gene048962 | PPI |
| MS.gene00279 | MS.gene25769 | PPI |
| MS.gene00279 | MS.gene52346 | PPI |
| MS.gene00279 | MS.gene52388 | PPI |
| MS.gene00279 | MS.gene90281 | PPI |
| MS.gene00279 | MS.gene009331 | PPI |
| MS.gene00279 | MS.gene02646 | PPI |
| MS.gene55828 | MS.gene00279 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00279.t1 | MTR_2g436310 | 97.872 | 188 | 4 | 0 | 1 | 188 | 1 | 188 | 1.94e-131 | 369 |
| MS.gene00279.t1 | MTR_5g067530 | 43.889 | 180 | 101 | 0 | 5 | 184 | 10 | 189 | 5.24e-49 | 160 |
| MS.gene00279.t1 | MTR_6g007633 | 48.588 | 177 | 87 | 3 | 8 | 181 | 11 | 186 | 2.16e-48 | 157 |
| MS.gene00279.t1 | MTR_8g096320 | 43.646 | 181 | 101 | 1 | 5 | 184 | 9 | 189 | 1.51e-45 | 150 |
| MS.gene00279.t1 | MTR_6g007623 | 46.893 | 177 | 90 | 3 | 8 | 181 | 8 | 183 | 1.82e-45 | 150 |
| MS.gene00279.t1 | MTR_6g007637 | 45.198 | 177 | 93 | 3 | 8 | 181 | 12 | 187 | 7.04e-45 | 149 |
| MS.gene00279.t1 | MTR_1g029380 | 43.011 | 186 | 103 | 2 | 1 | 184 | 1 | 185 | 1.97e-44 | 147 |
| MS.gene00279.t1 | MTR_3g098930 | 44.199 | 181 | 99 | 2 | 5 | 184 | 10 | 189 | 2.18e-44 | 146 |
| MS.gene00279.t1 | MTR_3g098910 | 41.436 | 181 | 105 | 1 | 5 | 184 | 9 | 189 | 2.54e-44 | 149 |
| MS.gene00279.t1 | MTR_3g080990 | 45.251 | 179 | 96 | 1 | 8 | 184 | 11 | 189 | 7.58e-44 | 147 |
| MS.gene00279.t1 | MTR_3g089125 | 44.134 | 179 | 95 | 3 | 9 | 184 | 9 | 185 | 1.31e-43 | 145 |
| MS.gene00279.t1 | MTR_8g099730 | 45.556 | 180 | 96 | 1 | 8 | 185 | 21 | 200 | 5.25e-42 | 142 |
| MS.gene00279.t1 | MTR_7g007490 | 37.853 | 177 | 110 | 0 | 9 | 185 | 14 | 190 | 2.37e-41 | 140 |
| MS.gene00279.t1 | MTR_8g096310 | 38.889 | 180 | 109 | 1 | 6 | 184 | 10 | 189 | 8.42e-41 | 139 |
| MS.gene00279.t1 | MTR_5g092600 | 37.791 | 172 | 107 | 0 | 14 | 185 | 18 | 189 | 2.78e-40 | 137 |
| MS.gene00279.t1 | MTR_2g007890 | 39.773 | 176 | 105 | 1 | 9 | 184 | 16 | 190 | 5.25e-40 | 137 |
| MS.gene00279.t1 | MTR_1g028460 | 42.541 | 181 | 100 | 1 | 8 | 184 | 9 | 189 | 7.69e-40 | 136 |
| MS.gene00279.t1 | MTR_7g405730 | 42.778 | 180 | 103 | 0 | 5 | 184 | 9 | 188 | 1.51e-39 | 135 |
| MS.gene00279.t1 | MTR_3g090940 | 42.857 | 182 | 98 | 3 | 8 | 184 | 9 | 189 | 7.44e-39 | 133 |
| MS.gene00279.t1 | MTR_3g090950 | 40.884 | 181 | 103 | 1 | 8 | 184 | 9 | 189 | 2.56e-38 | 132 |
| MS.gene00279.t1 | MTR_7g405710 | 39.444 | 180 | 109 | 0 | 5 | 184 | 9 | 188 | 1.85e-35 | 125 |
| MS.gene00279.t1 | MTR_8g042490 | 42.077 | 183 | 95 | 4 | 9 | 185 | 18 | 195 | 5.01e-35 | 123 |
| MS.gene00279.t1 | MTR_4g106990 | 41.718 | 163 | 91 | 3 | 25 | 184 | 35 | 196 | 2.56e-31 | 114 |
| MS.gene00279.t1 | MTR_2g073190 | 41.341 | 179 | 100 | 3 | 9 | 184 | 18 | 194 | 8.62e-31 | 118 |
| MS.gene00279.t1 | MTR_6g007610 | 38.220 | 191 | 93 | 5 | 8 | 181 | 11 | 193 | 3.92e-30 | 110 |
| MS.gene00279.t1 | MTR_8g096320 | 41.353 | 133 | 77 | 1 | 53 | 184 | 2 | 134 | 5.91e-27 | 101 |
| MS.gene00279.t1 | MTR_8g099730 | 40.741 | 135 | 78 | 1 | 53 | 185 | 2 | 136 | 2.52e-23 | 92.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00279.t1 | AT4G15920 | 60.106 | 188 | 74 | 1 | 1 | 187 | 1 | 188 | 7.54e-80 | 238 |
| MS.gene00279.t1 | AT3G16690 | 55.319 | 188 | 83 | 1 | 1 | 187 | 1 | 188 | 3.96e-72 | 218 |
| MS.gene00279.t1 | AT3G16690 | 55.319 | 188 | 83 | 1 | 1 | 187 | 36 | 223 | 8.59e-72 | 218 |
| MS.gene00279.t1 | AT3G16690 | 54.362 | 149 | 67 | 1 | 1 | 148 | 1 | 149 | 4.32e-54 | 171 |
| MS.gene00279.t1 | AT5G50800 | 42.541 | 181 | 103 | 1 | 5 | 184 | 9 | 189 | 3.25e-48 | 159 |
| MS.gene00279.t1 | AT1G21460 | 45.556 | 180 | 91 | 3 | 9 | 184 | 9 | 185 | 2.40e-47 | 154 |
| MS.gene00279.t1 | AT4G10850 | 44.086 | 186 | 97 | 3 | 8 | 188 | 13 | 196 | 3.04e-47 | 155 |
| MS.gene00279.t1 | AT5G40260 | 44.022 | 184 | 97 | 5 | 5 | 183 | 8 | 190 | 6.11e-47 | 153 |
| MS.gene00279.t1 | AT1G21460 | 45.556 | 180 | 91 | 3 | 9 | 184 | 9 | 185 | 9.08e-47 | 154 |
| MS.gene00279.t1 | AT5G40260 | 44.022 | 184 | 97 | 5 | 5 | 183 | 8 | 190 | 1.22e-46 | 153 |
| MS.gene00279.t1 | AT3G48740 | 40.000 | 180 | 108 | 0 | 5 | 184 | 11 | 190 | 7.03e-46 | 152 |
| MS.gene00279.t1 | AT4G25010 | 41.243 | 177 | 103 | 1 | 9 | 184 | 13 | 189 | 1.85e-45 | 151 |
| MS.gene00279.t1 | AT5G62850 | 41.053 | 190 | 105 | 4 | 1 | 184 | 1 | 189 | 4.57e-45 | 148 |
| MS.gene00279.t1 | AT2G39060 | 40.761 | 184 | 109 | 0 | 5 | 188 | 9 | 192 | 1.62e-44 | 148 |
| MS.gene00279.t1 | AT5G62850 | 41.053 | 190 | 105 | 4 | 1 | 184 | 1 | 189 | 3.65e-44 | 147 |
| MS.gene00279.t1 | AT5G23660 | 39.444 | 180 | 109 | 0 | 5 | 184 | 11 | 190 | 7.17e-44 | 147 |
| MS.gene00279.t1 | AT5G13170 | 39.227 | 181 | 109 | 1 | 5 | 184 | 11 | 191 | 1.20e-42 | 144 |
| MS.gene00279.t1 | AT5G53190 | 42.162 | 185 | 95 | 4 | 8 | 184 | 9 | 189 | 6.31e-42 | 142 |
| MS.gene00279.t1 | AT1G66770 | 40.860 | 186 | 103 | 4 | 8 | 188 | 13 | 196 | 3.15e-40 | 137 |
| MS.gene00279.t1 | AT5G50790 | 37.500 | 176 | 110 | 0 | 9 | 184 | 13 | 188 | 8.93e-40 | 137 |
| MS.gene00279.t1 | AT3G28007 | 41.899 | 179 | 100 | 3 | 9 | 184 | 12 | 189 | 1.70e-34 | 122 |
| MS.gene00279.t1 | AT3G14770 | 36.313 | 179 | 111 | 2 | 9 | 185 | 18 | 195 | 2.94e-28 | 105 |
Find 34 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCAATTCTTGCTGGGATTT+TGG | 0.124715 | 2.2:-52748945 | MS.gene00279:CDS |
| CTTGTTCGTGATTCCATTCT+TGG | 0.150295 | 2.2:-52699567 | MS.gene00279:CDS |
| TATAATGCAGACCAACATTT+TGG | 0.198727 | 2.2:-52749667 | MS.gene00279:intron |
| TACCTTGTGGCCACTGTTAA+TGG | 0.201020 | 2.2:-52749536 | MS.gene00279:CDS |
| AGTTTCTTTGTTGGAGTTAT+AGG | 0.210484 | 2.2:-52749963 | MS.gene00279:intron |
| TCTTCATTGTGGACTTACTA+TGG | 0.279770 | 2.2:-52749578 | MS.gene00279:CDS |
| TTGTTCGTGATTCCATTCTT+GGG | 0.316813 | 2.2:-52699566 | MS.gene00279:CDS |
| GTGGCCACTGTTAATGGCTT+TGG | 0.323328 | 2.2:-52749530 | MS.gene00279:CDS |
| GCTTTGAACATTCTTATGTA+TGG | 0.363700 | 2.2:-52748833 | MS.gene00279:CDS |
| GTGAAGACTGCAATTCTTGC+TGG | 0.366290 | 2.2:-52748953 | MS.gene00279:CDS |
| TAGTGGTGCTGTTGGTATCA+TGG | 0.401798 | 2.2:-52748861 | MS.gene00279:CDS |
| GCAGATCCAAGTTTCTTTGT+TGG | 0.416684 | 2.2:-52749972 | MS.gene00279:CDS |
| TGGCTCACCTCTTGCTGTCA+TGG | 0.454725 | 2.2:-52748813 | MS.gene00279:intron |
| TTAAGCAATGTGCATATGTA+AGG | 0.458717 | 2.2:+52749602 | None:intergenic |
| TACTATGGAACTATAAAAGC+TGG | 0.493273 | 2.2:-52749563 | MS.gene00279:CDS |
| ACCAGAGCTTACCCCAAGAA+TGG | 0.501782 | 2.2:+52699554 | None:intergenic |
| TTTATTATTCTCCAAAATGT+TGG | 0.503864 | 2.2:+52749656 | None:intergenic |
| TACTACACAATTAGCATTGG+AGG | 0.507198 | 2.2:-52748894 | MS.gene00279:CDS |
| TGAAGACTGCAATTCTTGCT+GGG | 0.514788 | 2.2:-52748952 | MS.gene00279:CDS |
| ATAACTCCAACAAAGAAACT+TGG | 0.519013 | 2.2:+52749966 | None:intergenic |
| AGTGGTGCTGTTGGTATCAT+GGG | 0.532404 | 2.2:-52748860 | MS.gene00279:CDS |
| GATTACTACACAATTAGCAT+TGG | 0.536087 | 2.2:-52748897 | MS.gene00279:CDS |
| TGTTCGTGATTCCATTCTTG+GGG | 0.555609 | 2.2:-52699565 | None:intergenic |
| AAACTTACCATGACAGCAAG+AGG | 0.581451 | 2.2:+52748806 | None:intergenic |
| ACTACACAATTAGCATTGGA+GGG | 0.582927 | 2.2:-52748893 | MS.gene00279:CDS |
| GAAGCTCGTAGTGGTGCTGT+TGG | 0.583483 | 2.2:-52748869 | MS.gene00279:CDS |
| AGCCATTAACAGTGGCCACA+AGG | 0.600324 | 2.2:+52749534 | None:intergenic |
| CATTGCTTAACTCTTCATTG+TGG | 0.620597 | 2.2:-52749589 | MS.gene00279:CDS |
| TAATGGCTTTGGCATCGTGG+TGG | 0.662663 | 2.2:-52749519 | MS.gene00279:CDS |
| TGTTAATGGCTTTGGCATCG+TGG | 0.667290 | 2.2:-52749522 | MS.gene00279:CDS |
| TTGGAGGGAGAAGCTCGTAG+TGG | 0.699075 | 2.2:-52748878 | MS.gene00279:CDS |
| AAAAGCTGGAGAATACCTTG+TGG | 0.701844 | 2.2:-52749549 | MS.gene00279:CDS |
| TTGTAACTTTCAGAAAACGG+TGG | 0.711959 | 2.2:-52699676 | MS.gene00279:intron |
| GATGCCAAAGCCATTAACAG+TGG | 0.716694 | 2.2:+52749526 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.2 | gene | 52699566 | 52749997 | 52699566 | ID=MS.gene00279 |
| chr2.2 | mRNA | 52699566 | 52749997 | 52699566 | ID=MS.gene00279.t1;Parent=MS.gene00279 |
| chr2.2 | exon | 52749964 | 52749997 | 52749964 | ID=MS.gene00279.t1.exon1;Parent=MS.gene00279.t1 |
| chr2.2 | CDS | 52749964 | 52749997 | 52749964 | ID=cds.MS.gene00279.t1;Parent=MS.gene00279.t1 |
| chr2.2 | exon | 52749782 | 52749818 | 52749782 | ID=MS.gene00279.t1.exon2;Parent=MS.gene00279.t1 |
| chr2.2 | CDS | 52749782 | 52749818 | 52749782 | ID=cds.MS.gene00279.t1;Parent=MS.gene00279.t1 |
| chr2.2 | exon | 52749469 | 52749679 | 52749469 | ID=MS.gene00279.t1.exon3;Parent=MS.gene00279.t1 |
| chr2.2 | CDS | 52749469 | 52749679 | 52749469 | ID=cds.MS.gene00279.t1;Parent=MS.gene00279.t1 |
| chr2.2 | exon | 52748814 | 52748975 | 52748814 | ID=MS.gene00279.t1.exon4;Parent=MS.gene00279.t1 |
| chr2.2 | CDS | 52748814 | 52748975 | 52748814 | ID=cds.MS.gene00279.t1;Parent=MS.gene00279.t1 |
| chr2.2 | exon | 52699566 | 52699685 | 52699566 | ID=MS.gene00279.t1.exon5;Parent=MS.gene00279.t1 |
| chr2.2 | CDS | 52699566 | 52699685 | 52699566 | ID=cds.MS.gene00279.t1;Parent=MS.gene00279.t1 |
| Gene Sequence |
| Protein sequence |
