AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene069975

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069975.t1 MTR_3g116120 98.137 483 9 0 1 483 1 483 0.0 976
MS.gene069975.t1 MTR_5g085170 81.988 483 37 3 1 483 1 433 0.0 767
MS.gene069975.t1 MTR_6g053260 52.807 481 79 11 1 480 1 334 3.88e-128 388
MS.gene069975.t1 MTR_3g099000 27.392 533 263 12 47 469 6 524 7.39e-54 189
MS.gene069975.t1 MTR_3g088480 25.388 516 282 18 56 474 170 679 4.89e-33 133
MS.gene069975.t1 MTR_4g069830 29.643 280 148 6 51 281 133 412 1.63e-28 120
MS.gene069975.t1 MTR_4g069830 29.643 280 148 6 51 281 133 412 1.84e-28 120
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069975.t1 AT2G33610 52.174 437 187 8 48 474 39 463 4.15e-147 429
MS.gene069975.t1 AT2G47620 27.642 492 275 11 51 465 7 494 1.52e-37 144
MS.gene069975.t1 AT1G21700 24.423 520 297 17 35 472 158 663 1.58e-37 147
MS.gene069975.t1 AT2G47620 31.419 296 153 6 51 300 7 298 1.03e-33 132
MS.gene069975.t1 AT4G34430 30.686 277 146 7 51 281 139 415 3.07e-25 110
MS.gene069975.t1 AT4G34430 30.686 277 146 7 51 281 139 415 3.22e-25 110
MS.gene069975.t1 AT4G34430 30.686 277 146 7 51 281 139 415 3.40e-25 110
MS.gene069975.t1 AT4G34430 30.686 277 146 7 51 281 139 415 3.40e-25 110

Find 121 sgRNAs with CRISPR-Local

Find 183 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGGCGTCGCTAGAGGTTT+TGG 0.216425 3.2:+91770347 None:intergenic
CTTGAAGCTATTACGAATTT+CGG 0.268562 3.2:-91769426 MS.gene069975:CDS
GAGGCACGACGGTTCTGTTT+GGG 0.304033 3.2:+91770475 None:intergenic
GGCTGCTTTCCTTTCTGCTT+TGG 0.304195 3.2:-91768833 MS.gene069975:CDS
GGTTCTGGTAATGGGACTTC+AGG 0.304961 3.2:+91770369 None:intergenic
TTCTGAGGTTACAGAAGTTC+AGG 0.308719 3.2:-91768395 MS.gene069975:intron
GCCACCGGAGGCGGCGGTGG+TGG 0.310149 3.2:+91770396 None:intergenic
TTTGTAAACCCTAGGGTTCT+TGG 0.330782 3.2:+91770242 None:intergenic
AGGCGGCGGTGGTGGGTTCG+AGG 0.332048 3.2:+91770404 None:intergenic
GCTTTCCTTTCTGCTTTGGC+AGG 0.337459 3.2:-91768829 MS.gene069975:CDS
GAGAAGGCAATCATGTAAAA+AGG 0.339330 3.2:-91768130 MS.gene069975:CDS
TTTATCCGACGCAACAGTTT+CGG 0.342652 3.2:+91769224 None:intergenic
ATTCGTGGAAATTATCGAAT+TGG 0.350506 3.2:-91769531 MS.gene069975:CDS
CCTAGGGTTCTTGGAAGATT+CGG 0.357797 3.2:+91770251 None:intergenic
GGAGGCACGACGGTTCTGTT+TGG 0.358696 3.2:+91770474 None:intergenic
CGCTCAGTAAACCTCTCAAA+TGG 0.370402 3.2:-91770059 MS.gene069975:CDS
CTAGGGTTCTTGGAAGATTC+GGG 0.380857 3.2:+91770252 None:intergenic
ATTCGATGCTGCGTCGGGTT+TGG 0.382011 3.2:+91770023 None:intergenic
GACGGTTCTGTTTGGGGTTG+AGG 0.382202 3.2:+91770482 None:intergenic
AGCCGGAGGTTGAGGTGTTC+CGG 0.383049 3.2:+91770440 None:intergenic
TTCGCCACCGGAGGCGGCGG+TGG 0.405270 3.2:+91770393 None:intergenic
TGAGGATTTAGACGTGCTAA+TGG 0.416011 3.2:-91768246 MS.gene069975:CDS
TTACGAATTTCGGTGATGAT+TGG 0.419721 3.2:-91769416 MS.gene069975:CDS
CCTTTCTGCTTTGGCAGGGA+CGG 0.421374 3.2:-91768824 MS.gene069975:CDS
TCTTCGTTTGCTCCAACTGC+TGG 0.436192 3.2:+91768213 None:intergenic
CCACCGGAGGCGGCGGTGGT+GGG 0.442186 3.2:+91770397 None:intergenic
GAAAGAACCTGAGCCATGAT+AGG 0.443755 3.2:+91769156 None:intergenic
TCAGGTTTCGCCACCGGAGG+CGG 0.443862 3.2:+91770387 None:intergenic
ACGGATTTCGCATTCGTGAA+TGG 0.445293 3.2:+91770281 None:intergenic
GGTGGTGGGTTCGAGGGAGG+CGG 0.448251 3.2:+91770411 None:intergenic
GCGGAATTCGATGCTGCGTC+GGG 0.449596 3.2:+91770018 None:intergenic
TGGAAGATTCGGGAATGTTA+CGG 0.464080 3.2:+91770262 None:intergenic
TTGCACGCAATAAAGATCCT+TGG 0.465240 3.2:+91768461 None:intergenic
AGGGTTGCTCATCAAGTTGT+TGG 0.467288 3.2:-91769390 MS.gene069975:CDS
GAACCCACCACCGCCGCCTC+CGG 0.468860 3.2:-91770400 MS.gene069975:CDS
GTGCCTCCGTCTTCCGCCAC+CGG 0.470261 3.2:-91770459 MS.gene069975:CDS
ATCTGAAGCATTACCACCAT+TGG 0.471038 3.2:+91768489 None:intergenic
GATTCACATTCAGCATCAAC+GGG 0.474269 3.2:+91769246 None:intergenic
AATCAACTAGAATTAATTAC+CGG 0.474504 3.2:-91768747 MS.gene069975:CDS
TCTAGTTGATTTGTAAACAT+TGG 0.479041 3.2:+91768759 None:intergenic
CGAACCAACGTCACCGACTA+GGG 0.480955 3.2:+91770143 None:intergenic
GGCGGTGGTGGGTTCGAGGG+AGG 0.482062 3.2:+91770408 None:intergenic
ATTCTCTTAGCTTCTTTGAC+AGG 0.485476 3.2:+91769976 None:intergenic
CAAAATTGATAAGCTTGTCC+TGG 0.486513 3.2:+91768267 None:intergenic
CTGTAGTATTTGTAAACCCT+AGG 0.490342 3.2:+91770234 None:intergenic
TCGAACCAACGTCACCGACT+AGG 0.492371 3.2:+91770142 None:intergenic
TCTGCTCCTCGCATTGATGA+TGG 0.492996 3.2:-91769288 MS.gene069975:CDS
CCGAATCTTCCAAGAACCCT+AGG 0.500165 3.2:-91770251 MS.gene069975:CDS
GCCACTCAAGATGGTTCTCA+TGG 0.500382 3.2:-91770308 MS.gene069975:CDS
GGTGTTCCGGTGGCGGAAGA+CGG 0.500983 3.2:+91770453 None:intergenic
AGGTTGAGGTGTTCCGGTGG+CGG 0.501069 3.2:+91770446 None:intergenic
GGGACTTCAGGTTTCGCCAC+CGG 0.504355 3.2:+91770381 None:intergenic
TGCGGAATTCGATGCTGCGT+CGG 0.506487 3.2:+91770017 None:intergenic
GGTTTCGCCACCGGAGGCGG+CGG 0.509368 3.2:+91770390 None:intergenic
GGAGTAGTAGCCGGAGGTTG+AGG 0.511155 3.2:+91770432 None:intergenic
CACCGGAACACCTCAACCTC+CGG 0.512230 3.2:-91770442 MS.gene069975:CDS
TCTTTGACAGGCGCCGGAGA+AGG 0.514796 3.2:+91769988 None:intergenic
CTTTCCTTTCTGCTTTGGCA+GGG 0.515547 3.2:-91768828 MS.gene069975:CDS
TGATTGCCTTCTCCGGTCGT+TGG 0.518632 3.2:+91768140 None:intergenic
GATGCTGCTGTTGCATCCAA+TGG 0.519252 3.2:-91768505 MS.gene069975:CDS
GGCTTGCTTTGCCTGTGAGA+AGG 0.520661 3.2:-91769923 MS.gene069975:intron
ATAAAAGATTGAGCTGATCA+AGG 0.521081 3.2:+91768180 None:intergenic
ACGAGCAACACATTCCTTGT+CGG 0.522938 3.2:+91769362 None:intergenic
ATCAATGCGAGGAGCAGACT+CGG 0.528293 3.2:+91769293 None:intergenic
GGATGCTAGCAATCCTATCA+TGG 0.529881 3.2:-91769169 MS.gene069975:CDS
TGCAGAAACTGATCACCAAA+AGG 0.531795 3.2:+91769327 None:intergenic
AGAACCATCTTGAGTGGCTA+GGG 0.532947 3.2:+91770313 None:intergenic
TAGCAATCCTATCATGGCTC+AGG 0.533854 3.2:-91769163 MS.gene069975:intron
TCTTTATCTTCCCATTTGAG+AGG 0.534057 3.2:+91770048 None:intergenic
TGTAGTATTTGTAAACCCTA+GGG 0.534357 3.2:+91770235 None:intergenic
AAAACATCTGCACCAACGAC+CGG 0.535739 3.2:-91768152 MS.gene069975:CDS
TCTGCACCAACGACCGGAGA+AGG 0.536832 3.2:-91768146 MS.gene069975:CDS
GGATTCACATTCAGCATCAA+CGG 0.539105 3.2:+91769245 None:intergenic
GCTCAGTAAACCTCTCAAAT+GGG 0.539806 3.2:-91770058 MS.gene069975:CDS
CCCACCACCGCCGCCTCCGG+TGG 0.540370 3.2:-91770397 MS.gene069975:CDS
TTAGCTTCTTTGACAGGCGC+CGG 0.542076 3.2:+91769982 None:intergenic
ATTTCGGTGATGATTGGAAG+AGG 0.544103 3.2:-91769410 MS.gene069975:CDS
AACTAAACTGACCTTCTCAC+AGG 0.545557 3.2:+91769912 None:intergenic
GCTAGGGACTAGTATTAAGT+TGG 0.546014 3.2:+91770329 None:intergenic
GGTTTACTGAGCGAAGAAGA+AGG 0.548651 3.2:+91770069 None:intergenic
GAGGGAGGCGGAGTAGTAGC+CGG 0.549317 3.2:+91770423 None:intergenic
TTCTGCTTTGGCAGGGACGG+AGG 0.550159 3.2:-91768821 MS.gene069975:CDS
TGCAAATTTACAGCTTGAGA+AGG 0.556628 3.2:-91768443 MS.gene069975:CDS
TTCCGCAGAATCTCCTTCTC+CGG 0.556844 3.2:-91770001 MS.gene069975:CDS
CGAATCTTCCAAGAACCCTA+GGG 0.558609 3.2:-91770250 MS.gene069975:CDS
CCTTGCTGCAATGTATTCCT+CGG 0.560596 3.2:+91768718 None:intergenic
ATGTATTCCTCGGAAATGAC+CGG 0.561368 3.2:+91768728 None:intergenic
TTGCTCATCAAGTTGTTGGT+AGG 0.563248 3.2:-91769386 MS.gene069975:CDS
CCGTCCCTGCCAAAGCAGAA+AGG 0.564962 3.2:+91768824 None:intergenic
GGCGGCGGTGGTGGGTTCGA+GGG 0.565038 3.2:+91770405 None:intergenic
AGGCACGACGGTTCTGTTTG+GGG 0.565942 3.2:+91770476 None:intergenic
TATTAAGTTGGCGTCGCTAG+AGG 0.569207 3.2:+91770341 None:intergenic
TAATACAGAATTCAAGAGAG+TGG 0.570887 3.2:-91769502 MS.gene069975:CDS
GAGAACCATCTTGAGTGGCT+AGG 0.580360 3.2:+91770312 None:intergenic
GACGGAGGTTGCACAAGCTG+CGG 0.582049 3.2:-91768806 MS.gene069975:CDS
AATGCTTCAGATTCTATCCA+AGG 0.586687 3.2:-91768478 MS.gene069975:CDS
CAAGAGAGTGGAGATAAGTG+AGG 0.590112 3.2:-91769490 MS.gene069975:CDS
ACTTCAGGTTTCGCCACCGG+AGG 0.593361 3.2:+91770384 None:intergenic
TAGTCCCTAGCCACTCAAGA+TGG 0.598302 3.2:-91770317 MS.gene069975:CDS
TTAATTACCGGTCATTTCCG+AGG 0.609744 3.2:-91768735 MS.gene069975:CDS
TTTCGGTGATGATTGGAAGA+GGG 0.613337 3.2:-91769409 MS.gene069975:CDS
AATGTGTCTCACACCTCTTG+CGG 0.617103 3.2:-91769190 MS.gene069975:CDS
GGATTGCTAGCATCCGCAAG+AGG 0.621759 3.2:+91769177 None:intergenic
CCACTCAAGATGGTTCTCAT+GGG 0.624877 3.2:-91770307 MS.gene069975:CDS
CGCCGGAGAAGGAGATTCTG+CGG 0.634496 3.2:+91769999 None:intergenic
CCGAGGAATACATTGCAGCA+AGG 0.638324 3.2:-91768718 MS.gene069975:intron
TGAATCCGAAACTGTTGCGT+CGG 0.639096 3.2:-91769229 MS.gene069975:CDS
GGAGGCGGAGTAGTAGCCGG+AGG 0.642192 3.2:+91770426 None:intergenic
CGGAGGTTGAGGTGTTCCGG+TGG 0.643233 3.2:+91770443 None:intergenic
CCCATGAGAACCATCTTGAG+TGG 0.645166 3.2:+91770307 None:intergenic
GTTCCGGTGGCGGAAGACGG+AGG 0.648161 3.2:+91770456 None:intergenic
GAAGCTAAGAGAATCTGTAG+TGG 0.654963 3.2:-91769967 MS.gene069975:CDS
AGTTGTTGGTAGGACCGACA+AGG 0.658858 3.2:-91769376 MS.gene069975:CDS
TCACATTCAGCATCAACGGG+AGG 0.659217 3.2:+91769249 None:intergenic
GATGTTCGCAAAACCCTAGT+CGG 0.661918 3.2:-91770156 MS.gene069975:CDS
ATAATATGACGTTGTGTGCG+AGG 0.667737 3.2:-91769560 MS.gene069975:CDS
TGAGAAGGAAGAATCAGACG+TGG 0.680762 3.2:-91768428 MS.gene069975:CDS
AAAACCCTAGTCGGTGACGT+TGG 0.681388 3.2:-91770147 MS.gene069975:CDS
GGCGGAAGACGGAGGCACGA+CGG 0.688333 3.2:+91770464 None:intergenic
GCTGCTGTTGCATCCAATGG+TGG 0.695124 3.2:-91768502 MS.gene069975:CDS
TCAGAACCATCATCAATGCG+AGG 0.698974 3.2:+91769282 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAAAATATTTTCATGATT+TGG + chr3.2:91768852-91768871 None:intergenic 10.0%
!!! ATAATTTGAAATGTGAATTT+CGG - chr3.2:91769024-91769043 MS.gene069975:intron 15.0%
!! AATCAACTAGAATTAATTAC+CGG - chr3.2:91769866-91769885 MS.gene069975:intron 20.0%
!! AATCTGTCATATATCTTAAT+AGG - chr3.2:91769975-91769994 MS.gene069975:CDS 20.0%
!! ATCTGTCATATATCTTAATA+GGG - chr3.2:91769976-91769995 MS.gene069975:CDS 20.0%
!! ATTTGTGTGTTATAATGTTT+GGG - chr3.2:91769532-91769551 MS.gene069975:CDS 20.0%
!! CCAATCATATATGTATTTAA+TGG + chr3.2:91768941-91768960 None:intergenic 20.0%
!! CCATTAAATACATATATGAT+TGG - chr3.2:91768938-91768957 MS.gene069975:intron 20.0%
!! GAAATTCACATTTCAAATTA+TGG + chr3.2:91769025-91769044 None:intergenic 20.0%
!! TAAACAATTCAAAGAAAACA+AGG + chr3.2:91769498-91769517 None:intergenic 20.0%
!! TAATAATCTTTGCTGAATAT+TGG - chr3.2:91770297-91770316 MS.gene069975:CDS 20.0%
!! TTATTTGTTCAAATTATTGC+AGG - chr3.2:91769759-91769778 MS.gene069975:intron 20.0%
!!! AAAATATTTTCATGATTTGG+CGG + chr3.2:91768849-91768868 None:intergenic 20.0%
! AAATCATTTGTCATTCAACA+AGG + chr3.2:91769713-91769732 None:intergenic 25.0%
! AATCATTTGTCATTCAACAA+GGG + chr3.2:91769712-91769731 None:intergenic 25.0%
! AGAACAATTGGAATTTGATT+CGG + chr3.2:91769608-91769627 None:intergenic 25.0%
! CATTTGTGTGTTATAATGTT+TGG - chr3.2:91769531-91769550 MS.gene069975:CDS 25.0%
! TCTATAGATGAAAAATATCC+AGG - chr3.2:91770328-91770347 MS.gene069975:CDS 25.0%
! TTAAAATTAGTTGCATGACA+TGG - chr3.2:91768867-91768886 MS.gene069975:intron 25.0%
! TTTGTGTGTTATAATGTTTG+GGG - chr3.2:91769533-91769552 MS.gene069975:CDS 25.0%
!!! CGATTTAGTGTTTTATTTGA+TGG - chr3.2:91770256-91770275 MS.gene069975:CDS 25.0%
!!! TCTAGTTGATTTGTAAACAT+TGG + chr3.2:91769857-91769876 None:intergenic 25.0%
AATCCGAGTTTAAAGATTTC+CGG + chr3.2:91770061-91770080 None:intergenic 30.0%
ATAAAAGATTGAGCTGATCA+AGG + chr3.2:91770436-91770455 None:intergenic 30.0%
ATTCGTGGAAATTATCGAAT+TGG - chr3.2:91769082-91769101 MS.gene069975:intron 30.0%
CTGTCTTAATTCTTAGTAGT+TGG - chr3.2:91769635-91769654 MS.gene069975:intron 30.0%
CTTGAAGCTATTACGAATTT+CGG - chr3.2:91769187-91769206 MS.gene069975:CDS 30.0%
GAATCAAATTCCAATTGTTC+TGG - chr3.2:91769607-91769626 MS.gene069975:intron 30.0%
TAATACAGAATTCAAGAGAG+TGG - chr3.2:91769111-91769130 MS.gene069975:intron 30.0%
TAATAGGGTTTATCTCGAAA+TGG - chr3.2:91769991-91770010 MS.gene069975:CDS 30.0%
TGTAGTATTTGTAAACCCTA+GGG + chr3.2:91768381-91768400 None:intergenic 30.0%
TTGATTACGTGTAGCATTAA+AGG - chr3.2:91769573-91769592 MS.gene069975:intron 30.0%
! GTACACTTCATTAGTTTGAA+TGG - chr3.2:91770033-91770052 MS.gene069975:CDS 30.0%
! TCGGATTTTCTCATTAGAAA+TGG - chr3.2:91770074-91770093 MS.gene069975:CDS 30.0%
! TTCATTAGTTTGAATGGTTC+CGG - chr3.2:91770039-91770058 MS.gene069975:CDS 30.0%
! TTTTCAGATTCAATCCTAAC+AGG - chr3.2:91768419-91768438 MS.gene069975:CDS 30.0%
!! AAAAAATGAATGGACTGAGA+AGG - chr3.2:91769150-91769169 MS.gene069975:intron 30.0%
!! CTTTGATTTTCTTGAAGCTT+GGG - chr3.2:91768504-91768523 MS.gene069975:CDS 30.0%
!! TCTTTGATTTTCTTGAAGCT+TGG - chr3.2:91768503-91768522 MS.gene069975:CDS 30.0%
!! TTTGATTTTCTTGAAGCTTG+GGG - chr3.2:91768505-91768524 MS.gene069975:CDS 30.0%
AAATCCTATAATCTTAGCCC+AGG + chr3.2:91769937-91769956 None:intergenic 35.0%
AATGCTTCAGATTCTATCCA+AGG - chr3.2:91770135-91770154 MS.gene069975:CDS 35.0%
ATAACCTGGGCTAAGATTAT+AGG - chr3.2:91769930-91769949 MS.gene069975:CDS 35.0%
ATTCTCTTAGCTTCTTTGAC+AGG + chr3.2:91768640-91768659 None:intergenic 35.0%
CAAAATTGATAAGCTTGTCC+TGG + chr3.2:91770349-91770368 None:intergenic 35.0%
CTGTAGTATTTGTAAACCCT+AGG + chr3.2:91768382-91768401 None:intergenic 35.0%
CTTTGCTGAATATTGGATGT+TGG - chr3.2:91770304-91770323 MS.gene069975:CDS 35.0%
GAATCTGAAAAACTTGACGA+TGG + chr3.2:91768410-91768429 None:intergenic 35.0%
GAGAAGGCAATCATGTAAAA+AGG - chr3.2:91770483-91770502 MS.gene069975:CDS 35.0%
GTGAGAATGACATATAACCT+GGG - chr3.2:91769917-91769936 MS.gene069975:intron 35.0%
GTGAGGAGACAAAAAATGAA+TGG - chr3.2:91769140-91769159 MS.gene069975:intron 35.0%
GTTCCGGAAATCTTTAAACT+CGG - chr3.2:91770055-91770074 MS.gene069975:CDS 35.0%
TCTTTATCTTCCCATTTGAG+AGG + chr3.2:91768568-91768587 None:intergenic 35.0%
TGCAAATTTACAGCTTGAGA+AGG - chr3.2:91770170-91770189 MS.gene069975:CDS 35.0%
TTACGAATTTCGGTGATGAT+TGG - chr3.2:91769197-91769216 MS.gene069975:CDS 35.0%
! GGACAAGCTTATCAATTTTG+AGG - chr3.2:91770349-91770368 MS.gene069975:CDS 35.0%
! GTGAATGTGATTTTCCTGTT+AGG + chr3.2:91768436-91768455 None:intergenic 35.0%
! TTTTACATGATTGCCTTCTC+CGG + chr3.2:91770483-91770502 None:intergenic 35.0%
!!! CGTTTTTTAGAGCTTCCTTT+TGG - chr3.2:91769271-91769290 MS.gene069975:CDS 35.0%
!!! TGGCGGAATACAAAGTTTTT+TGG + chr3.2:91768832-91768851 None:intergenic 35.0%
AACTAAACTGACCTTCTCAC+AGG + chr3.2:91768704-91768723 None:intergenic 40.0%
ATAATATGACGTTGTGTGCG+AGG - chr3.2:91769053-91769072 MS.gene069975:intron 40.0%
ATGTATTCCTCGGAAATGAC+CGG + chr3.2:91769888-91769907 None:intergenic 40.0%
ATTTCGGTGATGATTGGAAG+AGG - chr3.2:91769203-91769222 MS.gene069975:CDS 40.0%
CGTGGAAAAAGCAATTTCTG+AGG - chr3.2:91770203-91770222 MS.gene069975:CDS 40.0%
CTGCAAAAATCTCTGTGTCA+TGG - chr3.2:91768669-91768688 MS.gene069975:intron 40.0%
GAAGCTAAGAGAATCTGTAG+TGG - chr3.2:91768646-91768665 MS.gene069975:intron 40.0%
GAAGTGTACACGATGAACAT+TGG + chr3.2:91770022-91770041 None:intergenic 40.0%
GATTCACATTCAGCATCAAC+GGG + chr3.2:91769370-91769389 None:intergenic 40.0%
GCTCAGTAAACCTCTCAAAT+GGG - chr3.2:91768555-91768574 MS.gene069975:intron 40.0%
GGATTCACATTCAGCATCAA+CGG + chr3.2:91769371-91769390 None:intergenic 40.0%
GGTGAGAATGACATATAACC+TGG - chr3.2:91769916-91769935 MS.gene069975:intron 40.0%
TGAGGATTTAGACGTGCTAA+TGG - chr3.2:91770367-91770386 MS.gene069975:CDS 40.0%
TGCAGAAACTGATCACCAAA+AGG + chr3.2:91769289-91769308 None:intergenic 40.0%
TGGAAGATTCGGGAATGTTA+CGG + chr3.2:91768354-91768373 None:intergenic 40.0%
TTAATGACTGTGCTCTGACA+CGG - chr3.2:91769661-91769680 MS.gene069975:intron 40.0%
TTAATTACCGGTCATTTCCG+AGG - chr3.2:91769878-91769897 MS.gene069975:intron 40.0%
TTGCACGCAATAAAGATCCT+TGG + chr3.2:91770155-91770174 None:intergenic 40.0%
TTTATCCGACGCAACAGTTT+CGG + chr3.2:91769392-91769411 None:intergenic 40.0%
TTTCGGTGATGATTGGAAGA+GGG - chr3.2:91769204-91769223 MS.gene069975:CDS 40.0%
TTTGTAAACCCTAGGGTTCT+TGG + chr3.2:91768374-91768393 None:intergenic 40.0%
TTTGTCATTCAACAAGGGAC+TGG + chr3.2:91769707-91769726 None:intergenic 40.0%
! ATCTGAAGCATTACCACCAT+TGG + chr3.2:91770127-91770146 None:intergenic 40.0%
! GCAGATTGATGGTTTCTTAG+CGG - chr3.2:91768889-91768908 MS.gene069975:intron 40.0%
! GCTAGGGACTAGTATTAAGT+TGG + chr3.2:91768287-91768306 None:intergenic 40.0%
! TTGCTCATCAAGTTGTTGGT+AGG - chr3.2:91769227-91769246 MS.gene069975:CDS 40.0%
!! TTCTGAGGTTACAGAAGTTC+AGG - chr3.2:91770218-91770237 MS.gene069975:CDS 40.0%
!!! AGGTTTTGGGTTCTGGTAAT+GGG + chr3.2:91768255-91768274 None:intergenic 40.0%
AAAACATCTGCACCAACGAC+CGG - chr3.2:91770461-91770480 MS.gene069975:CDS 45.0%
AATGTGTCTCACACCTCTTG+CGG - chr3.2:91769423-91769442 MS.gene069975:CDS 45.0%
ACGAGCAACACATTCCTTGT+CGG + chr3.2:91769254-91769273 None:intergenic 45.0%
ACGGATTTCGCATTCGTGAA+TGG + chr3.2:91768335-91768354 None:intergenic 45.0%
AGAACCATCTTGAGTGGCTA+GGG + chr3.2:91768303-91768322 None:intergenic 45.0%
CAAGAGAGTGGAGATAAGTG+AGG - chr3.2:91769123-91769142 MS.gene069975:intron 45.0%
CCACTCAAGATGGTTCTCAT+GGG - chr3.2:91768306-91768325 MS.gene069975:intron 45.0%
CCTAGGGTTCTTGGAAGATT+CGG + chr3.2:91768365-91768384 None:intergenic 45.0%
CCTTGCTGCAATGTATTCCT+CGG + chr3.2:91769898-91769917 None:intergenic 45.0%
CGAATCTTCCAAGAACCCTA+GGG - chr3.2:91768363-91768382 MS.gene069975:intron 45.0%
CGCTCAGTAAACCTCTCAAA+TGG - chr3.2:91768554-91768573 MS.gene069975:intron 45.0%
CTAGGGTTCTTGGAAGATTC+GGG + chr3.2:91768364-91768383 None:intergenic 45.0%
CTTTCCTTTCTGCTTTGGCA+GGG - chr3.2:91769785-91769804 MS.gene069975:intron 45.0%
GAAAGAACCTGAGCCATGAT+AGG + chr3.2:91769460-91769479 None:intergenic 45.0%
GACAGCGATTCCAGAACAAT+TGG + chr3.2:91769620-91769639 None:intergenic 45.0%
GATGTTCGCAAAACCCTAGT+CGG - chr3.2:91768457-91768476 MS.gene069975:CDS 45.0%
GGATGCTAGCAATCCTATCA+TGG - chr3.2:91769444-91769463 MS.gene069975:CDS 45.0%
GGTTTACTGAGCGAAGAAGA+AGG + chr3.2:91768547-91768566 None:intergenic 45.0%
TAGCAATCCTATCATGGCTC+AGG - chr3.2:91769450-91769469 MS.gene069975:CDS 45.0%
TCAGAACCATCATCAATGCG+AGG + chr3.2:91769334-91769353 None:intergenic 45.0%
TCATGGCTCAGGTTCTTTCT+TGG - chr3.2:91769461-91769480 MS.gene069975:CDS 45.0%
TGAATCCGAAACTGTTGCGT+CGG - chr3.2:91769384-91769403 MS.gene069975:CDS 45.0%
TGAGAAGGAAGAATCAGACG+TGG - chr3.2:91770185-91770204 MS.gene069975:CDS 45.0%
TGCATGACATGGCAGATTGA+TGG - chr3.2:91768878-91768897 MS.gene069975:intron 45.0%
! AGGGTTGCTCATCAAGTTGT+TGG - chr3.2:91769223-91769242 MS.gene069975:CDS 45.0%
! GACTAGGGTTTTGCGAACAT+CGG + chr3.2:91768458-91768477 None:intergenic 45.0%
!! TATTAAGTTGGCGTCGCTAG+AGG + chr3.2:91768275-91768294 None:intergenic 45.0%
!! TGTGCGAGGTGTTTTATTCG+TGG - chr3.2:91769067-91769086 MS.gene069975:intron 45.0%
!!! GAGGTTTTGGGTTCTGGTAA+TGG + chr3.2:91768256-91768275 None:intergenic 45.0%
AAAACCCTAGTCGGTGACGT+TGG - chr3.2:91768466-91768485 MS.gene069975:CDS 50.0%
ATCAATGCGAGGAGCAGACT+CGG + chr3.2:91769323-91769342 None:intergenic 50.0%
CCCATGAGAACCATCTTGAG+TGG + chr3.2:91768309-91768328 None:intergenic 50.0%
CCGAATCTTCCAAGAACCCT+AGG - chr3.2:91768362-91768381 MS.gene069975:intron 50.0%
CCGAGGAATACATTGCAGCA+AGG - chr3.2:91769895-91769914 MS.gene069975:intron 50.0%
GAGAACCATCTTGAGTGGCT+AGG + chr3.2:91768304-91768323 None:intergenic 50.0%
GATGCTGCTGTTGCATCCAA+TGG - chr3.2:91770108-91770127 MS.gene069975:CDS 50.0%
GCCACTCAAGATGGTTCTCA+TGG - chr3.2:91768305-91768324 MS.gene069975:intron 50.0%
GCTTTCCTTTCTGCTTTGGC+AGG - chr3.2:91769784-91769803 MS.gene069975:intron 50.0%
GGAAAAAGAACGCCAGCAGT+TGG - chr3.2:91770388-91770407 MS.gene069975:CDS 50.0%
TAGTCCCTAGCCACTCAAGA+TGG - chr3.2:91768296-91768315 MS.gene069975:intron 50.0%
TCACATTCAGCATCAACGGG+AGG + chr3.2:91769367-91769386 None:intergenic 50.0%
TCTTCGTTTGCTCCAACTGC+TGG + chr3.2:91770403-91770422 None:intergenic 50.0%
TTAGCTTCTTTGACAGGCGC+CGG + chr3.2:91768634-91768653 None:intergenic 50.0%
TTCCGCAGAATCTCCTTCTC+CGG - chr3.2:91768612-91768631 MS.gene069975:intron 50.0%
! AGTTGTTGGTAGGACCGACA+AGG - chr3.2:91769237-91769256 MS.gene069975:CDS 50.0%
! GGCTGCTTTCCTTTCTGCTT+TGG - chr3.2:91769780-91769799 MS.gene069975:intron 50.0%
! TCTGCTCCTCGCATTGATGA+TGG - chr3.2:91769325-91769344 MS.gene069975:CDS 50.0%
! TTGGCGTCGCTAGAGGTTTT+GGG + chr3.2:91768268-91768287 None:intergenic 50.0%
!! GGTTCTGGTAATGGGACTTC+AGG + chr3.2:91768247-91768266 None:intergenic 50.0%
!!! TCGCTAGAGGTTTTGGGTTC+TGG + chr3.2:91768262-91768281 None:intergenic 50.0%
AGGCACGACGGTTCTGTTTG+GGG + chr3.2:91768140-91768159 None:intergenic 55.0%
ATTCGATGCTGCGTCGGGTT+TGG + chr3.2:91768593-91768612 None:intergenic 55.0%
CCTTTCTGCTTTGGCAGGGA+CGG - chr3.2:91769789-91769808 MS.gene069975:intron 55.0%
GAGGCACGACGGTTCTGTTT+GGG + chr3.2:91768141-91768160 None:intergenic 55.0%
GCTGCTGTTGCATCCAATGG+TGG - chr3.2:91770111-91770130 MS.gene069975:CDS 55.0%
GGATTGCTAGCATCCGCAAG+AGG + chr3.2:91769439-91769458 None:intergenic 55.0%
TGATTGCCTTCTCCGGTCGT+TGG + chr3.2:91770476-91770495 None:intergenic 55.0%
TGCGGAATTCGATGCTGCGT+CGG + chr3.2:91768599-91768618 None:intergenic 55.0%
! CGAACCAACGTCACCGACTA+GGG + chr3.2:91768473-91768492 None:intergenic 55.0%
! GACGGTTCTGTTTGGGGTTG+AGG + chr3.2:91768134-91768153 None:intergenic 55.0%
! GGCTTGCTTTGCCTGTGAGA+AGG - chr3.2:91768690-91768709 MS.gene069975:intron 55.0%
! GTTGGCGTCGCTAGAGGTTT+TGG + chr3.2:91768269-91768288 None:intergenic 55.0%
! TCGAACCAACGTCACCGACT+AGG + chr3.2:91768474-91768493 None:intergenic 55.0%
CACCGGAACACCTCAACCTC+CGG - chr3.2:91768171-91768190 MS.gene069975:CDS 60.0%
CCGTCCCTGCCAAAGCAGAA+AGG + chr3.2:91769792-91769811 None:intergenic 60.0%
CGCCGGAGAAGGAGATTCTG+CGG + chr3.2:91768617-91768636 None:intergenic 60.0%
GACGGAGGTTGCACAAGCTG+CGG - chr3.2:91769807-91769826 MS.gene069975:intron 60.0%
GCGGAATTCGATGCTGCGTC+GGG + chr3.2:91768598-91768617 None:intergenic 60.0%
GGAGTAGTAGCCGGAGGTTG+AGG + chr3.2:91768184-91768203 None:intergenic 60.0%
TCTGCACCAACGACCGGAGA+AGG - chr3.2:91770467-91770486 MS.gene069975:CDS 60.0%
TTCTGCTTTGGCAGGGACGG+AGG - chr3.2:91769792-91769811 MS.gene069975:intron 60.0%
! ACTTCAGGTTTCGCCACCGG+AGG + chr3.2:91768232-91768251 None:intergenic 60.0%
! AGCCGGAGGTTGAGGTGTTC+CGG + chr3.2:91768176-91768195 None:intergenic 60.0%
! AGGTTGAGGTGTTCCGGTGG+CGG + chr3.2:91768170-91768189 None:intergenic 60.0%
! GGAGGCACGACGGTTCTGTT+TGG + chr3.2:91768142-91768161 None:intergenic 60.0%
! GGGACTTCAGGTTTCGCCAC+CGG + chr3.2:91768235-91768254 None:intergenic 60.0%
!! TCTTTGACAGGCGCCGGAGA+AGG + chr3.2:91768628-91768647 None:intergenic 60.0%
GAGGGAGGCGGAGTAGTAGC+CGG + chr3.2:91768193-91768212 None:intergenic 65.0%
TCAGGTTTCGCCACCGGAGG+CGG + chr3.2:91768229-91768248 None:intergenic 65.0%
! CGGAGGTTGAGGTGTTCCGG+TGG + chr3.2:91768173-91768192 None:intergenic 65.0%
!! GGTGTTCCGGTGGCGGAAGA+CGG + chr3.2:91768163-91768182 None:intergenic 65.0%
GGAGGCGGAGTAGTAGCCGG+AGG + chr3.2:91768190-91768209 None:intergenic 70.0%
GTGCCTCCGTCTTCCGCCAC+CGG - chr3.2:91768154-91768173 MS.gene069975:CDS 70.0%
! GGCGGAAGACGGAGGCACGA+CGG + chr3.2:91768152-91768171 None:intergenic 70.0%
!! GGTGGTGGGTTCGAGGGAGG+CGG + chr3.2:91768205-91768224 None:intergenic 70.0%
!! GTTCCGGTGGCGGAAGACGG+AGG + chr3.2:91768160-91768179 None:intergenic 70.0%
GAACCCACCACCGCCGCCTC+CGG - chr3.2:91768213-91768232 MS.gene069975:CDS 75.0%
GGTTTCGCCACCGGAGGCGG+CGG + chr3.2:91768226-91768245 None:intergenic 75.0%
!! AGGCGGCGGTGGTGGGTTCG+AGG + chr3.2:91768212-91768231 None:intergenic 75.0%
!! GGCGGCGGTGGTGGGTTCGA+GGG + chr3.2:91768211-91768230 None:intergenic 75.0%
!! GGCGGTGGTGGGTTCGAGGG+AGG + chr3.2:91768208-91768227 None:intergenic 75.0%
! CCACCGGAGGCGGCGGTGGT+GGG + chr3.2:91768219-91768238 None:intergenic 80.0%
! TTCGCCACCGGAGGCGGCGG+TGG + chr3.2:91768223-91768242 None:intergenic 80.0%
! CCCACCACCGCCGCCTCCGG+TGG - chr3.2:91768216-91768235 MS.gene069975:CDS 85.0%
! GCCACCGGAGGCGGCGGTGG+TGG + chr3.2:91768220-91768239 None:intergenic 85.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 91768124 91770511 91768124 ID=MS.gene069975
chr3.2 mRNA 91768124 91770511 91768124 ID=MS.gene069975.t1;Parent=MS.gene069975
chr3.2 exon 91769924 91770511 91769924 ID=MS.gene069975.t1.exon1;Parent=MS.gene069975.t1
chr3.2 CDS 91769924 91770511 91769924 ID=cds.MS.gene069975.t1;Parent=MS.gene069975.t1
chr3.2 exon 91769164 91769583 91769164 ID=MS.gene069975.t1.exon2;Parent=MS.gene069975.t1
chr3.2 CDS 91769164 91769583 91769164 ID=cds.MS.gene069975.t1;Parent=MS.gene069975.t1
chr3.2 exon 91768719 91768854 91768719 ID=MS.gene069975.t1.exon3;Parent=MS.gene069975.t1
chr3.2 CDS 91768719 91768854 91768719 ID=cds.MS.gene069975.t1;Parent=MS.gene069975.t1
chr3.2 exon 91768396 91768526 91768396 ID=MS.gene069975.t1.exon4;Parent=MS.gene069975.t1
chr3.2 CDS 91768396 91768526 91768396 ID=cds.MS.gene069975.t1;Parent=MS.gene069975.t1
chr3.2 exon 91768124 91768300 91768124 ID=MS.gene069975.t1.exon5;Parent=MS.gene069975.t1
chr3.2 CDS 91768124 91768300 91768124 ID=cds.MS.gene069975.t1;Parent=MS.gene069975.t1
Gene Sequence

>MS.gene069975

ATGGCGACCTCAACCCCAAACAGAACCGTCGTGCCTCCGTCTTCCGCCACCGGAACACCTCAACCTCCGGCTACTACTCCGCCTCCCTCGAACCCACCACCGCCGCCTCCGGTGGCGAAACCTGAAGTCCCATTACCAGAACCCAAAACCTCTAGCGACGCCAACTTAATACTAGTCCCTAGCCACTCAAGATGGTTCTCATGGGATTCCATTCACGAATGCGAAATCCGTAACATTCCCGAATCTTCCAAGAACCCTAGGGTTTACAAATACTACAGAAACTCCATCGTCAAGTTTTTCAGATTCAATCCTAACAGGAAAATCACATTCACCGATGTTCGCAAAACCCTAGTCGGTGACGTTGGTTCGATTCGAAGAGTCTTTGATTTTCTTGAAGCTTGGGGTTTAATCAATTATCATCCTTCTTCTTCGCTCAGTAAACCTCTCAAATGGGAAGATAAAGACACCAAACCCGACGCAGCATCGAATTCCGCAGAATCTCCTTCTCCGGCGCCTGTCAAAGAAGCTAAGAGAATCTGTAGTGGCTGCAAAAATCTCTGTGTCATGGCTTGCTTTGCCTGTGAGAAGGTCAGTTTAGTTAAAGATTTTTTACACTTTCAATCAATTGTGATTCTCGTATTTTTTTAAGAGAAAACAGAAGATGCATTGATGATGTAAAATAGTAAAATAATTTTACACTGACATCCAAAAAACTTTGTATTCCGCCAAATCATGAAAATATTTTAAAATTAGTTGCATGACATGGCAGATTGATGGTTTCTTAGCGGATGACAGTGTAAAACTCAATTTATCACCATTAAATACATATATGATTGGCTGTGATTTTTTTGACTGTGTAAAACTTTTATGCTGAGAGTGTATTGAAATTAAACTAATTCCATAATTTGAAATGTGAATTTCGGTGCAGAATAATATGACGTTGTGTGCGAGGTGTTTTATTCGTGGAAATTATCGAATTGGTATGAGTAATACAGAATTCAAGAGAGTGGAGATAAGTGAGGAGACAAAAAATGAATGGACTGAGAAGGAAACACTGAATCTTCTTGAAGCTATTACGAATTTCGGTGATGATTGGAAGAGGGTTGCTCATCAAGTTGTTGGTAGGACCGACAAGGAATGTGTTGCTCGTTTTTTAGAGCTTCCTTTTGGTGATCAGTTTCTGCATTATCAACACTCCGAGTCTGCTCCTCGCATTGATGATGGTTCTGATCAGTTGAAGCCTCCCGTTGATGCTGAATGTGAATCCGAAACTGTTGCGTCGGATAAATCTAGTAAGAGAATGTGTCTCACACCTCTTGCGGATGCTAGCAATCCTATCATGGCTCAGGTTCTTTCTTGGCTTAAAATTATCCTTGTTTTCTTTGAATTGTTTATTGTTATTGATTACATTTGTGTGTTATAATGTTTGGGGAAAAAATGAAACTTCATTTGATTACGTGTAGCATTAAAGGAACATGATACCGAATCAAATTCCAATTGTTCTGGAATCGCTGTCTTAATTCTTAGTAGTTGGTTGTTAATGACTGTGCTCTGACACGGTCTTTTGAAATTGATTATAGCCAGTCCCTTGTTGAATGACAAATGATTTCTTTCAAGTTCTTGTGTCTTAACAATTTATTTGTTCAAATTATTGCAGGCTGCTTTCCTTTCTGCTTTGGCAGGGACGGAGGTTGCACAAGCTGCGGCTCAAGCTGCTCTGACAAGTTTGTCCAATGTTTACAAATCAACTAGAATTAATTACCGGTCATTTCCGAGGAATACATTGCAGCAAGGTGAGAATGACATATAACCTGGGCTAAGATTATAGGATTTGAATGAATTTAAGCTGTCAATCTGTCATATATCTTAATAGGGTTTATCTCGAAATGGATGAACCAATGTTCATCGTGTACACTTCATTAGTTTGAATGGTTCCGGAAATCTTTAAACTCGGATTTTCTCATTAGAAATGGCGTTTCATGCAGATGCTGCTGTTGCATCCAATGGTGGTAATGCTTCAGATTCTATCCAAGGATCTTTATTGCGTGCAAATTTACAGCTTGAGAAGGAAGAATCAGACGTGGAAAAAGCAATTTCTGAGGTTACAGAAGTTCAGGTCTGTCATGTGTTAGCGATTTAGTGTTTTATTTGATGGTGTTTGTACTTATTAGTTTAATAATCTTTGCTGAATATTGGATGTTGGCTCTATAGATGAAAAATATCCAGGACAAGCTTATCAATTTTGAGGATTTAGACGTGCTAATGGAAAAAGAACGCCAGCAGTTGGAGCAAACGAAGAGTTTGTTTTTCCTTGATCAGCTCAATCTTTTATTTCGTAAAACATCTGCACCAACGACCGGAGAAGGCAATCATGTAAAAAGGAATTGA
Protein sequence

>MS.gene069975.t1

MATSTPNRTVVPPSSATGTPQPPATTPPPSNPPPPPPVAKPEVPLPEPKTSSDANLILVPSHSRWFSWDSIHECEIRNIPESSKNPRVYKYYRNSIVKFFRFNPNRKITFTDVRKTLVGDVGSIRRVFDFLEAWGLINYHPSSSLSKPLKWEDKDTKPDAASNSAESPSPAPVKEAKRICSGCKNLCVMACFACEKNNMTLCARCFIRGNYRIGMSNTEFKRVEISEETKNEWTEKETLNLLEAITNFGDDWKRVAHQVVGRTDKECVARFLELPFGDQFLHYQHSESAPRIDDGSDQLKPPVDAECESETVASDKSSKRMCLTPLADASNPIMAQAAFLSALAGTEVAQAAAQAALTSLSNVYKSTRINYRSFPRNTLQQDAAVASNGGNASDSIQGSLLRANLQLEKEESDVEKAISEVTEVQMKNIQDKLINFEDLDVLMEKERQQLEQTKSLFFLDQLNLLFRKTSAPTTGEGNHVKRN