AlfalfaGEDB | MS.gene94315

M. sativa cultivar XinJiangDaYe / MS.gene94315

KEGG: K11649
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF00249.34 PF16495.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94315.t1	XP_003616869.1	88.8	276	23	1	1	268	158	433	3.60E-126	461.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94315.t1	Q84JG2	46.5	286	124	5	1	261	184	465	7.7e-61	235.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94315.t1	G7KH84	88.8	276	23	1	1	268	158	433	2.6e-126	461.1

TFs/TRs:

Gene ID	Type	Classification
MS.gene94315.t1	TF	MYB-related

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene000128	MS.gene94315	PPI
MS.gene58116	MS.gene94315	PPI
MS.gene99648	MS.gene94315	PPI
MS.gene94315	MS.gene006556	PPI
MS.gene94315	MS.gene04748	PPI
MS.gene030661	MS.gene94315	PPI
MS.gene28432	MS.gene94315	PPI
MS.gene94315	MS.gene73873	PPI
MS.gene97703	MS.gene94315	PPI
MS.gene014197	MS.gene94315	PPI
MS.gene94315	MS.gene05905	PPI
MS.gene94315	MS.gene64164	PPI
MS.gene91631	MS.gene94315	PPI
MS.gene021887	MS.gene94315	PPI
MS.gene009155	MS.gene94315	PPI
MS.gene94315	MS.gene64820	PPI
MS.gene94315	MS.gene67685	PPI
MS.gene94315	MS.gene027909	PPI
MS.gene39538	MS.gene94315	PPI
MS.gene78922	MS.gene94315	PPI
MS.gene071851	MS.gene94315	PPI
MS.gene47573	MS.gene94315	PPI
MS.gene065792	MS.gene94315	PPI
MS.gene94315	MS.gene063084	PPI
MS.gene28434	MS.gene94315	PPI
MS.gene94315	MS.gene030664	PPI
MS.gene97701	MS.gene94315	PPI
MS.gene057458	MS.gene94315	PPI
MS.gene059054	MS.gene94315	PPI
MS.gene063673	MS.gene94315	PPI
MS.gene031750	MS.gene94315	PPI
MS.gene065890	MS.gene94315	PPI
MS.gene068178	MS.gene94315	PPI
MS.gene94315	MS.gene017431	PPI
MS.gene94315	MS.gene70258	PPI
MS.gene006560	MS.gene94315	PPI
MS.gene94315	MS.gene056335	PPI
MS.gene94315	MS.gene58112	PPI
MS.gene66051	MS.gene94315	PPI
MS.gene67503	MS.gene94315	PPI
MS.gene94315	MS.gene23886	PPI
MS.gene030666	MS.gene94315	PPI
MS.gene94315	MS.gene68733	PPI
MS.gene031751	MS.gene94315	PPI
MS.gene049111	MS.gene94315	PPI
MS.gene027904	MS.gene94315	PPI
MS.gene00233	MS.gene94315	PPI
MS.gene70263	MS.gene94315	PPI
MS.gene94315	MS.gene024895	PPI
MS.gene94315	MS.gene26084	PPI
MS.gene94315	MS.gene78227	PPI
MS.gene006555	MS.gene94315	PPI
MS.gene065891	MS.gene94315	PPI
MS.gene65125	MS.gene94315	PPI
MS.gene29445	MS.gene94315	PPI
MS.gene26406	MS.gene94315	PPI
MS.gene94315	MS.gene78224	PPI
MS.gene89203	MS.gene94315	PPI
MS.gene068179	MS.gene94315	PPI
MS.gene006557	MS.gene94315	PPI
MS.gene92318	MS.gene94315	PPI
MS.gene015760	MS.gene94315	PPI
MS.gene58114	MS.gene94315	PPI
MS.gene78924	MS.gene94315	PPI
MS.gene58285	MS.gene94315	PPI
MS.gene94315	MS.gene70990	PPI
MS.gene94315	MS.gene28438	PPI
MS.gene94315	MS.gene91416	PPI
MS.gene94315	MS.gene70261	PPI
MS.gene94315	MS.gene80094	PPI
MS.gene038612	MS.gene94315	PPI
MS.gene027907	MS.gene94315	PPI
MS.gene68134	MS.gene94315	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94315.t1	MTR_5g085170	88.768	276	23	1	1	268	158	433	2.82e-171	480
MS.gene94315.t1	MTR_3g116120	83.729	295	21	2	1	268	189	483	2.28e-169	477
MS.gene94315.t1	MTR_6g053260	91.089	101	9	0	165	265	234	334	3.44e-53	183
MS.gene94315.t1	MTR_6g053260	83.333	96	8	1	9	96	135	230	1.12e-40	149
MS.gene94315.t1	MTR_3g099000	25.378	331	158	6	4	254	203	524	2.21e-19	87.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94315.t1	AT2G33610	46.503	286	124	5	1	261	184	465	5.53e-76	238
MS.gene94315.t1	AT1G21700	21.523	302	185	6	8	257	362	663	8.37e-18	83.2

Find 50 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGAAGCTATTACAAATTTC+GGG	0.188443	6.2:+43208330	MS.gene94315:CDS
CTTGAAGCTATTACAAATTT+CGG	0.191024	6.2:+43208329	MS.gene94315:CDS
GGCTGCTTTCCTTTCTGCTT+TGG	0.311179	6.2:+43208556	MS.gene94315:CDS
GCTTTCCTTTCTGCTTTGGC+AGG	0.337459	6.2:+43208560	MS.gene94315:CDS
AATGTGTCTCACACCTCTTA+TGG	0.384773	6.2:+43208508	MS.gene94315:CDS
TTACAAATTTCGGGGATGAT+TGG	0.415906	6.2:+43208339	MS.gene94315:CDS
ATTCGTGGAAACTATCGAAT+TGG	0.417271	6.2:+43208248	MS.gene94315:CDS
CCTTTCTGCTTTGGCAGGGA+CGG	0.421374	6.2:+43208565	MS.gene94315:CDS
TCTTCGTTTGCTCCAACTGC+TGG	0.436192	6.2:-43208889	None:intergenic
TCTTGCTGCAATGTATTCCT+TGG	0.449690	6.2:-43208671	None:intergenic
CAAAATTGATAAGCTTGTCC+TGG	0.455951	6.2:-43208835	None:intergenic
AGGGTTGCTCATCAAGTTGT+TGG	0.467288	6.2:+43208365	MS.gene94315:CDS
AAAGCAGCCTGAGCCATGAT+AGG	0.474193	6.2:-43208542	None:intergenic
AATCAACTAGAATTAATTAC+CGG	0.474504	6.2:+43208642	MS.gene94315:CDS
AGTTCAGATGAAAATAATCC+AGG	0.500753	6.2:+43208817	MS.gene94315:CDS
TTGCACGCAATAGAGATCCT+TGG	0.503227	6.2:-43208736	None:intergenic
GGATGCTAGCAATCCTATCA+TGG	0.508578	6.2:+43208529	MS.gene94315:CDS
CTTTCCTTTCTGCTTTGGCA+GGG	0.515547	6.2:+43208561	MS.gene94315:CDS
ATAAAAGATTGAGCTGATCA+AGG	0.521081	6.2:-43208922	None:intergenic
TAGCAATCCTATCATGGCTC+AGG	0.529443	6.2:+43208535	MS.gene94315:CDS
GGATTGCTAGCATCCATAAG+AGG	0.529525	6.2:-43208521	None:intergenic
GGCTTCAACTGATCGCCAAA+AGG	0.531669	6.2:-43208428	None:intergenic
ACGCGCAACACATTCCTTGT+CGG	0.532524	6.2:-43208393	None:intergenic
TGGAAACTATCGAATTGGTA+TGG	0.532567	6.2:+43208253	MS.gene94315:CDS
GAATCAGAAACTGTTGCGTC+GGG	0.534084	6.2:+43208470	MS.gene94315:CDS
CGTCATATTATTCTTATCAC+AGG	0.538639	6.2:-43208207	None:intergenic
TGATTGCCTTCTCTGGTCGT+TGG	0.540914	6.2:-43208962	None:intergenic
TTAATTACCGGTCATTTCCA+AGG	0.542958	6.2:+43208654	MS.gene94315:CDS
ATTTCGGGGATGATTGGAAG+AGG	0.548838	6.2:+43208345	MS.gene94315:CDS
GATTCACATTCAGCATCAGC+GGG	0.549492	6.2:-43208452	None:intergenic
TTCTGCTTTGGCAGGGACGG+AGG	0.550159	6.2:+43208568	MS.gene94315:CDS
TGTGCGAGGTGTTATATTCG+TGG	0.552624	6.2:+43208233	MS.gene94315:CDS
GATGCTTCTGTTGCATGCGA+TGG	0.554502	6.2:+43208692	MS.gene94315:CDS
TGAGGATTTAGACGTGCTGA+TGG	0.555586	6.2:+43208856	MS.gene94315:CDS
TGCAAATTTACAGCTTGAGA+AGG	0.556628	6.2:+43208754	MS.gene94315:CDS
ATGTATTCCTTGGAAATGAC+CGG	0.559908	6.2:-43208661	None:intergenic
TGAATCAGAAACTGTTGCGT+CGG	0.561653	6.2:+43208469	MS.gene94315:CDS
TTGCTCATCAAGTTGTTGGT+AGG	0.563248	6.2:+43208369	MS.gene94315:CDS
CCGTCCCTGCCAAAGCAGAA+AGG	0.564962	6.2:-43208565	None:intergenic
AATGCTTCAGATTCTATCCA+AGG	0.586687	6.2:+43208719	MS.gene94315:CDS
TCTGCACCAACGACCAGAGA+AGG	0.588232	6.2:+43208956	MS.gene94315:CDS
TGATTCACATTCAGCATCAG+CGG	0.598353	6.2:-43208453	None:intergenic
GCTTCTGTTGCATGCGATGG+TGG	0.606537	6.2:+43208695	MS.gene94315:CDS
TTTCGGGGATGATTGGAAGA+GGG	0.613337	6.2:+43208346	MS.gene94315:CDS
TGAAGCTATTACAAATTTCG+GGG	0.621595	6.2:+43208331	MS.gene94315:CDS
AATTGGTATGGAGATAAGTG+AGG	0.626402	6.2:+43208265	MS.gene94315:CDS
TCACATTCAGCATCAGCGGG+AGG	0.629791	6.2:-43208449	None:intergenic
AGTTGTTGGTAGGACCGACA+AGG	0.658858	6.2:+43208379	MS.gene94315:CDS
ATAATATGACGTTGTGTGCG+AGG	0.667737	6.2:+43208219	MS.gene94315:CDS
TGAGAAGGAAGAATCAGACG+TGG	0.680762	6.2:+43208769	MS.gene94315:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATCAACTAGAATTAATTAC+CGG	+	chr6.2:43208642-43208661	MS.gene94315:CDS	20.0%
!	CTTGAAGCTATTACAAATTT+CGG	+	chr6.2:43208329-43208348	MS.gene94315:CDS	25.0%
!	TTGAAGCTATTACAAATTTC+GGG	+	chr6.2:43208330-43208349	MS.gene94315:CDS	25.0%
	AGTTCAGATGAAAATAATCC+AGG	+	chr6.2:43208817-43208836	MS.gene94315:CDS	30.0%
	ATAAAAGATTGAGCTGATCA+AGG	-	chr6.2:43208925-43208944	None:intergenic	30.0%
	CGTCATATTATTCTTATCAC+AGG	-	chr6.2:43208210-43208229	None:intergenic	30.0%
	TGAAGCTATTACAAATTTCG+GGG	+	chr6.2:43208331-43208350	MS.gene94315:CDS	30.0%
	AATGCTTCAGATTCTATCCA+AGG	+	chr6.2:43208719-43208738	MS.gene94315:CDS	35.0%
	AATTGGTATGGAGATAAGTG+AGG	+	chr6.2:43208265-43208284	MS.gene94315:CDS	35.0%
	ATGTATTCCTTGGAAATGAC+CGG	-	chr6.2:43208664-43208683	None:intergenic	35.0%
	ATTCGTGGAAACTATCGAAT+TGG	+	chr6.2:43208248-43208267	MS.gene94315:CDS	35.0%
	CAAAATTGATAAGCTTGTCC+TGG	-	chr6.2:43208838-43208857	None:intergenic	35.0%
	GTGAGGAGACAAAAACTAAA+TGG	+	chr6.2:43208282-43208301	MS.gene94315:CDS	35.0%
	TGCAAATTTACAGCTTGAGA+AGG	+	chr6.2:43208754-43208773	MS.gene94315:CDS	35.0%
	TGGAAACTATCGAATTGGTA+TGG	+	chr6.2:43208253-43208272	MS.gene94315:CDS	35.0%
	TTAATTACCGGTCATTTCCA+AGG	+	chr6.2:43208654-43208673	MS.gene94315:CDS	35.0%
	TTACAAATTTCGGGGATGAT+TGG	+	chr6.2:43208339-43208358	MS.gene94315:CDS	35.0%
!	GGACAAGCTTATCAATTTTG+AGG	+	chr6.2:43208838-43208857	MS.gene94315:CDS	35.0%
!	TTTTACATGATTGCCTTCTC+TGG	-	chr6.2:43208972-43208991	None:intergenic	35.0%
!!	AAAAACTAAATGGACTGAGG+AGG	+	chr6.2:43208292-43208311	MS.gene94315:CDS	35.0%
!!	CGTTTTCTAGAGATTCCTTT+TGG	+	chr6.2:43208413-43208432	MS.gene94315:CDS	35.0%
!!	GACAAAAACTAAATGGACTG+AGG	+	chr6.2:43208289-43208308	MS.gene94315:CDS	35.0%
	AATGTGTCTCACACCTCTTA+TGG	+	chr6.2:43208508-43208527	MS.gene94315:CDS	40.0%
	ATAATATGACGTTGTGTGCG+AGG	+	chr6.2:43208219-43208238	MS.gene94315:CDS	40.0%
	TCTTGCTGCAATGTATTCCT+TGG	-	chr6.2:43208674-43208693	None:intergenic	40.0%
	TGAATCAGAAACTGTTGCGT+CGG	+	chr6.2:43208469-43208488	MS.gene94315:CDS	40.0%
	TGATTCACATTCAGCATCAG+CGG	-	chr6.2:43208456-43208475	None:intergenic	40.0%
!	TTGCTCATCAAGTTGTTGGT+AGG	+	chr6.2:43208369-43208388	MS.gene94315:CDS	40.0%
!!	CGTGGAAAAAGCTATTTCTG+AGG	+	chr6.2:43208787-43208806	MS.gene94315:CDS	40.0%
	ATTTCGGGGATGATTGGAAG+AGG	+	chr6.2:43208345-43208364	MS.gene94315:CDS	45.0%
	CTTTCCTTTCTGCTTTGGCA+GGG	+	chr6.2:43208561-43208580	MS.gene94315:CDS	45.0%
	GAATCAGAAACTGTTGCGTC+GGG	+	chr6.2:43208470-43208489	MS.gene94315:CDS	45.0%
	GATTCACATTCAGCATCAGC+GGG	-	chr6.2:43208455-43208474	None:intergenic	45.0%
	GGATGCTAGCAATCCTATCA+TGG	+	chr6.2:43208529-43208548	MS.gene94315:CDS	45.0%
	GGATTGCTAGCATCCATAAG+AGG	-	chr6.2:43208524-43208543	None:intergenic	45.0%
	TAGCAATCCTATCATGGCTC+AGG	+	chr6.2:43208535-43208554	MS.gene94315:CDS	45.0%
	TGAGAAGGAAGAATCAGACG+TGG	+	chr6.2:43208769-43208788	MS.gene94315:CDS	45.0%
	TGAGGATTTAGACGTGCTGA+TGG	+	chr6.2:43208856-43208875	MS.gene94315:CDS	45.0%
	TTGCACGCAATAGAGATCCT+TGG	-	chr6.2:43208739-43208758	None:intergenic	45.0%
	TTTCGGGGATGATTGGAAGA+GGG	+	chr6.2:43208346-43208365	MS.gene94315:CDS	45.0%
!	AGGGTTGCTCATCAAGTTGT+TGG	+	chr6.2:43208365-43208384	MS.gene94315:CDS	45.0%
!	TGTGCGAGGTGTTATATTCG+TGG	+	chr6.2:43208233-43208252	MS.gene94315:CDS	45.0%
	AAAGCAGCCTGAGCCATGAT+AGG	-	chr6.2:43208545-43208564	None:intergenic	50.0%
	ACGCGCAACACATTCCTTGT+CGG	-	chr6.2:43208396-43208415	None:intergenic	50.0%
	GATGCTTCTGTTGCATGCGA+TGG	+	chr6.2:43208692-43208711	MS.gene94315:CDS	50.0%
	GCTTTCCTTTCTGCTTTGGC+AGG	+	chr6.2:43208560-43208579	MS.gene94315:CDS	50.0%
	GGAAAAAGAACGCCAGCAGT+TGG	+	chr6.2:43208877-43208896	MS.gene94315:CDS	50.0%
	GGCTTCAACTGATCGCCAAA+AGG	-	chr6.2:43208431-43208450	None:intergenic	50.0%
	TCTTCGTTTGCTCCAACTGC+TGG	-	chr6.2:43208892-43208911	None:intergenic	50.0%
	TGATTGCCTTCTCTGGTCGT+TGG	-	chr6.2:43208965-43208984	None:intergenic	50.0%
!	AGTTGTTGGTAGGACCGACA+AGG	+	chr6.2:43208379-43208398	MS.gene94315:CDS	50.0%
!	GGCTGCTTTCCTTTCTGCTT+TGG	+	chr6.2:43208556-43208575	MS.gene94315:CDS	50.0%
	CCTTTCTGCTTTGGCAGGGA+CGG	+	chr6.2:43208565-43208584	MS.gene94315:CDS	55.0%
	GCTTCTGTTGCATGCGATGG+TGG	+	chr6.2:43208695-43208714	MS.gene94315:CDS	55.0%
	TCACATTCAGCATCAGCGGG+AGG	-	chr6.2:43208452-43208471	None:intergenic	55.0%
	TCTGCACCAACGACCAGAGA+AGG	+	chr6.2:43208956-43208975	MS.gene94315:CDS	55.0%
	CCGTCCCTGCCAAAGCAGAA+AGG	-	chr6.2:43208568-43208587	None:intergenic	60.0%
	TTCTGCTTTGGCAGGGACGG+AGG	+	chr6.2:43208568-43208587	MS.gene94315:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.2	gene	43208194	43209000	43208194	ID=MS.gene94315
chr6.2	mRNA	43208194	43209000	43208194	ID=MS.gene94315.t1;Parent=MS.gene94315
chr6.2	exon	43208194	43209000	43208194	ID=MS.gene94315.t1.exon1;Parent=MS.gene94315.t1
chr6.2	CDS	43208194	43209000	43208194	ID=cds.MS.gene94315.t1;Parent=MS.gene94315.t1

Gene Sequence

>MS.gene94315

ATGGCTTGCTTTGCCTGTGATAAGAATAATATGACGTTGTGTGCGAGGTGTTATATTCGTGGAAACTATCGAATTGGTATGGAGATAAGTGAGGAGACAAAAACTAAATGGACTGAGGAGGAAACACTGAATCTTCTTGAAGCTATTACAAATTTCGGGGATGATTGGAAGAGGGTTGCTCATCAAGTTGTTGGTAGGACCGACAAGGAATGTGTTGCGCGTTTTCTAGAGATTCCTTTTGGCGATCAGTTGAAGCCTCCCGCTGATGCTGAATGTGAATCAGAAACTGTTGCGTCGGGTAAATCTAGTAAGAGAATGTGTCTCACACCTCTTATGGATGCTAGCAATCCTATCATGGCTCAGGCTGCTTTCCTTTCTGCTTTGGCAGGGACGGAGGTTGCACAAGCTGCAGCTCAAGCTGCTCTGACAAGTTTGTGCAATGTTTACAAATCAACTAGAATTAATTACCGGTCATTTCCAAGGAATACATTGCAGCAAGATGCTTCTGTTGCATGCGATGGTGGTAATGCTTCAGATTCTATCCAAGGATCTCTATTGCGTGCAAATTTACAGCTTGAGAAGGAAGAATCAGACGTGGAAAAAGCTATTTCTGAGGTTATAAAAGTTCAGATGAAAATAATCCAGGACAAGCTTATCAATTTTGAGGATTTAGACGTGCTGATGGAAAAAGAACGCCAGCAGTTGGAGCAAACGAAGAGTTTATATTTCCTTGATCAGCTCAATCTTTTATTTCGTAAAACATCTGCACCAACGACCAGAGAAGGCAATCATGTAAAAAGTAATTGA

Protein sequence

>MS.gene94315.t1

MACFACDKNNMTLCARCYIRGNYRIGMEISEETKTKWTEEETLNLLEAITNFGDDWKRVAHQVVGRTDKECVARFLEIPFGDQLKPPADAECESETVASGKSSKRMCLTPLMDASNPIMAQAAFLSALAGTEVAQAAAQAALTSLCNVYKSTRINYRSFPRNTLQQDASVACDGGNASDSIQGSLLRANLQLEKEESDVEKAISEVIKVQMKIIQDKLINFEDLDVLMEKERQQLEQTKSLYFLDQLNLLFRKTSAPTTREGNHVKSN