AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene070538

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070538.t1 MTR_5g085170 89.318 440 33 1 1 440 1 426 0.0 785
MS.gene070538.t1 MTR_3g116120 84.454 476 38 3 1 440 1 476 0.0 775
MS.gene070538.t1 MTR_6g053260 77.091 275 18 4 1 275 1 230 3.15e-130 392
MS.gene070538.t1 MTR_6g053260 92.632 95 7 0 344 438 234 328 7.67e-48 174
MS.gene070538.t1 MTR_3g099000 32.881 295 134 7 35 267 6 298 4.31e-38 145
MS.gene070538.t1 MTR_4g069830 27.027 296 157 9 39 280 133 423 2.73e-23 103
MS.gene070538.t1 MTR_4g069830 27.027 296 157 9 39 280 133 423 2.73e-23 103
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070538.t1 AT2G33610 50.348 431 180 8 36 438 39 463 4.94e-135 396
MS.gene070538.t1 AT1G21700 23.333 510 291 15 23 436 158 663 2.88e-36 142
MS.gene070538.t1 AT2G47620 27.846 492 250 15 39 429 7 494 2.86e-35 137
MS.gene070538.t1 AT2G47620 33.456 272 131 8 39 263 7 275 7.07e-31 123
MS.gene070538.t1 AT4G34430 30.249 281 145 8 39 268 139 419 7.45e-23 102
MS.gene070538.t1 AT4G34430 30.249 281 145 8 39 268 139 419 7.45e-23 102
MS.gene070538.t1 AT4G34430 30.249 281 145 8 39 268 139 419 7.72e-23 102
MS.gene070538.t1 AT4G34430 30.249 281 145 8 39 268 139 419 7.94e-23 102

Find 99 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAAGCTATTACAAATTTC+GGG 0.188443 5.4:+67258774 MS.gene070538:CDS
CTTGAAGCTATTACAAATTT+CGG 0.191024 5.4:+67258773 MS.gene070538:CDS
GTTGGCGTCGCCAGAGGTTT+TGG 0.205569 5.4:-67258207 None:intergenic
CGCTCAGTAAACCTCTGATA+TGG 0.237129 5.4:+67258495 MS.gene070538:CDS
GGCTGCTTTCCTTTCTGCTT+TGG 0.311179 5.4:+67259000 MS.gene070538:CDS
TTTGTAAACCCTAGGGTTCT+TGG 0.330782 5.4:-67258312 None:intergenic
GTCAGTCATCAATTACTTTC+CGG 0.330900 5.4:-67259413 None:intergenic
GCTTTCCTTTCTGCTTTGGC+AGG 0.337459 5.4:+67259004 MS.gene070538:CDS
CCTAGGGTTCTTGGAAGATT+CGG 0.357797 5.4:-67258303 None:intergenic
GGAGGCTCGACGGTTCTATT+TGG 0.364466 5.4:-67258116 None:intergenic
GGTTCTGGTAATGGGACTTC+CGG 0.374955 5.4:-67258185 None:intergenic
CTAGGGTTCTTGGAAGATTC+GGG 0.380857 5.4:-67258302 None:intergenic
AATGTGTCTCACACCTCTTA+TGG 0.384773 5.4:+67258952 MS.gene070538:CDS
ACCGCCTCCGGTGGCGAAAC+CGG 0.394595 5.4:+67258166 MS.gene070538:CDS
TTTACCAGACGCAACAGTTT+CGG 0.399478 5.4:-67258918 None:intergenic
TCTTCGTTTGCTCCAACTGC+TGG 0.408427 5.4:-67259333 None:intergenic
TCCGGTTTCGCCACCGGAGG+CGG 0.412679 5.4:-67258167 None:intergenic
ATCAATTACTTTCCGGTCGT+TGG 0.415852 5.4:-67259406 None:intergenic
TTACAAATTTCGGGGATGAT+TGG 0.415906 5.4:+67258783 MS.gene070538:CDS
GCAGATTCTCTTAGCTTCAT+TGG 0.416279 5.4:-67258582 None:intergenic
CCTTTCTGCTTTGGCAGGGA+CGG 0.421374 5.4:+67259009 MS.gene070538:CDS
AACACTGCAACCACCGCCTC+CGG 0.442861 5.4:+67258154 MS.gene070538:CDS
AGGAGATTCTGTAGAATTTG+AGG 0.449273 5.4:-67258546 None:intergenic
TCTTGCTGCAATGTATTCCT+TGG 0.449690 5.4:-67259115 None:intergenic
TTCTACAGAATCTCCTTCTC+TGG 0.459502 5.4:+67258553 MS.gene070538:CDS
TGGAAGATTCGGGAATGTTA+CGG 0.464080 5.4:-67258292 None:intergenic
AGGGTTGCTCATCAAGTTGT+TGG 0.467288 5.4:+67258809 MS.gene070538:CDS
ACGGATTTCACATTCGTGAA+TGG 0.467484 5.4:-67258273 None:intergenic
ATTCTCTTAGCTTCATTGGC+AGG 0.470496 5.4:-67258578 None:intergenic
TCTACTTGATTTGTAAACAT+TGG 0.471603 5.4:-67259074 None:intergenic
AAAGCAGCCTGAGCCATGAT+AGG 0.474193 5.4:-67258986 None:intergenic
GATTCACATTCAGCATCAAC+GGG 0.474269 5.4:-67258896 None:intergenic
CAAAATTGATAAGCTTGTCC+TGG 0.486513 5.4:-67259279 None:intergenic
ATTCGTGGGAGCTATCGAAT+TGG 0.488604 5.4:+67258668 MS.gene070538:CDS
CTGTAGTATTTGTAAACCCT+AGG 0.490342 5.4:-67258320 None:intergenic
GGGACTTCCGGTTTCGCCAC+CGG 0.493960 5.4:-67258173 None:intergenic
GCTCAGTAAACCTCTGATAT+GGG 0.498668 5.4:+67258496 MS.gene070538:CDS
CCGAATCTTCCAAGAACCCT+AGG 0.500165 5.4:+67258303 MS.gene070538:CDS
GCCACTCAAGATGGTTCTCA+TGG 0.500382 5.4:+67258246 MS.gene070538:CDS
TTGCACGCAATAGAGATCCT+TGG 0.503227 5.4:-67259180 None:intergenic
GGTTTCGCCACCGGAGGCGG+TGG 0.504542 5.4:-67258164 None:intergenic
GGATGCTAGCAATCCTATCA+TGG 0.508578 5.4:+67258973 MS.gene070538:CDS
AAAACAACTGCACCAACGAC+CGG 0.508881 5.4:+67259394 MS.gene070538:CDS
CGGTGGTTGCAGTGTTTCGA+CGG 0.513001 5.4:-67258147 None:intergenic
CTTTCCTTTCTGCTTTGGCA+GGG 0.515547 5.4:+67259005 MS.gene070538:CDS
TAGCAATCCTATCATGGCTC+AGG 0.529443 5.4:+67258979 MS.gene070538:CDS
GGATTGCTAGCATCCATAAG+AGG 0.529525 5.4:-67258965 None:intergenic
ACGAGCAACACATTCCTTGT+CGG 0.529759 5.4:-67258837 None:intergenic
GGCTTCAACTGATCGCCAAA+AGG 0.531669 5.4:-67258872 None:intergenic
TGTAGTATTTGTAAACCCTA+GGG 0.534357 5.4:-67258319 None:intergenic
AGAACCATCTTGAGTGGCTA+GGG 0.535119 5.4:-67258241 None:intergenic
TGGAACCAACGTCACCGACT+AGG 0.535199 5.4:-67258412 None:intergenic
CGTCGTATTATTCTTATCAC+AGG 0.538639 5.4:-67258627 None:intergenic
GGATTCACATTCAGCATCAA+CGG 0.539105 5.4:-67258897 None:intergenic
ATACAAGATTGAGCTGATCA+AGG 0.541570 5.4:-67259366 None:intergenic
ATCTGAAGCATTACCACCAT+CGG 0.545048 5.4:-67259152 None:intergenic
ATGTATTCCTTGGAAATGAT+CGG 0.546642 5.4:-67259105 None:intergenic
GATGCTTCTGTTGCATCCGA+TGG 0.547872 5.4:+67259136 MS.gene070538:CDS
GGTTTACTGAGCGAAGAAGA+AGG 0.548651 5.4:-67258485 None:intergenic
ATTTCGGGGATGATTGGAAG+AGG 0.548838 5.4:+67258789 MS.gene070538:CDS
ATCTTGAGTGGCTAGGGACA+AGG 0.549549 5.4:-67258235 None:intergenic
TTCTGCTTTGGCAGGGACGG+AGG 0.550159 5.4:+67259012 MS.gene070538:CDS
TTACCAGAACCCAAAACCTC+TGG 0.550434 5.4:+67258197 MS.gene070538:CDS
TTAATAACCGATCATTTCCA+AGG 0.554025 5.4:+67259098 MS.gene070538:CDS
TGAGGATTTAGACGTGCTGA+TGG 0.555586 5.4:+67259300 MS.gene070538:CDS
TGCAAATTTACAGCTTGAGA+AGG 0.556628 5.4:+67259198 MS.gene070538:CDS
CGAATCTTCCAAGAACCCTA+GGG 0.558609 5.4:+67258304 MS.gene070538:CDS
AAAATCAAAGACTCTTCGAA+TGG 0.560578 5.4:-67258432 None:intergenic
TCATTGGCAGGCACCAGAGA+AGG 0.560713 5.4:-67258566 None:intergenic
TTGCTCATCAAGTTGTTGGT+AGG 0.563248 5.4:+67258813 MS.gene070538:CDS
CCGTCCCTGCCAAAGCAGAA+AGG 0.564962 5.4:-67259009 None:intergenic
ATAATACGACGTTGTGTGCG+AGG 0.569617 5.4:+67258639 MS.gene070538:CDS
TAATACAGAATTCAAGAGAG+TGG 0.570887 5.4:+67258697 MS.gene070538:CDS
GAGAACCATCTTGAGTGGCT+AGG 0.580360 5.4:-67258242 None:intergenic
AGTGTTTCGACGGCGGAAGA+CGG 0.582593 5.4:-67258137 None:intergenic
GAATCCGAAACTGTTGCGTC+TGG 0.583173 5.4:+67258914 MS.gene070538:CDS
AATGCTTCAGATTCTATCCA+AGG 0.586687 5.4:+67259163 MS.gene070538:CDS
GAGAATCTGCAGTGTCTGCA+TGG 0.586827 5.4:+67258595 MS.gene070538:CDS
TTGTCCCTAGCCACTCAAGA+TGG 0.586862 5.4:+67258237 MS.gene070538:CDS
ACTGCAACCACCGCCTCCGG+TGG 0.586976 5.4:+67258157 MS.gene070538:CDS
TCTTTATCTTCCCATATCAG+AGG 0.587798 5.4:-67258506 None:intergenic
CAAGAGAGTGGAGATAAGTG+AGG 0.590112 5.4:+67258709 MS.gene070538:CDS
GACGGTTCTATTTGGCGTTG+AGG 0.590322 5.4:-67258108 None:intergenic
GGCGGAAGACGGAGGCTCGA+CGG 0.590550 5.4:-67258126 None:intergenic
GATTATGTTGGCGTCGCCAG+AGG 0.591406 5.4:-67258213 None:intergenic
GCTAGGGACAAGGATTATGT+TGG 0.592687 5.4:-67258225 None:intergenic
TTTCGGGGATGATTGGAAGA+GGG 0.613337 5.4:+67258790 MS.gene070538:CDS
AAAATCCTAGTCGGTGACGT+TGG 0.616879 5.4:+67258407 MS.gene070538:CDS
TGAAGCTATTACAAATTTCG+GGG 0.621595 5.4:+67258775 MS.gene070538:CDS
CCACTCAAGATGGTTCTCAT+GGG 0.624877 5.4:+67258247 MS.gene070538:CDS
TGGTTGCAGTGTTTCGACGG+CGG 0.630155 5.4:-67258144 None:intergenic
ACTTCCGGTTTCGCCACCGG+AGG 0.635420 5.4:-67258170 None:intergenic
GCTTCTGTTGCATCCGATGG+TGG 0.643243 5.4:+67259139 MS.gene070538:CDS
CCCATGAGAACCATCTTGAG+TGG 0.645166 5.4:-67258247 None:intergenic
GTTTCGACGGCGGAAGACGG+AGG 0.649104 5.4:-67258134 None:intergenic
AGTTGTTGGTAGGACCGACA+AGG 0.658858 5.4:+67258823 MS.gene070538:CDS
TCACATTCAGCATCAACGGG+AGG 0.659217 5.4:-67258893 None:intergenic
GATGTTCGCAAAATCCTAGT+CGG 0.674756 5.4:+67258398 MS.gene070538:CDS
TGAGAAGGAAGAATCAGACG+TGG 0.680762 5.4:+67259213 MS.gene070538:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CTTGAAGCTATTACAAATTT+CGG + chr5.4:67258773-67258792 MS.gene070538:CDS 25.0%
! TTGAAGCTATTACAAATTTC+GGG + chr5.4:67258774-67258793 MS.gene070538:CDS 25.0%
!!! TCTACTTGATTTGTAAACAT+TGG - chr5.4:67259077-67259096 None:intergenic 25.0%
AAAATCAAAGACTCTTCGAA+TGG - chr5.4:67258435-67258454 None:intergenic 30.0%
AGTTCAGATGAAAAATATCC+AGG + chr5.4:67259261-67259280 MS.gene070538:CDS 30.0%
ATGTATTCCTTGGAAATGAT+CGG - chr5.4:67259108-67259127 None:intergenic 30.0%
CAACAGAAAAATCACATTCA+CGG + chr5.4:67258376-67258395 MS.gene070538:CDS 30.0%
GTGAGGAGATAAAAAATGAA+TGG + chr5.4:67258726-67258745 MS.gene070538:CDS 30.0%
TAATACAGAATTCAAGAGAG+TGG + chr5.4:67258697-67258716 MS.gene070538:CDS 30.0%
TGAAGCTATTACAAATTTCG+GGG + chr5.4:67258775-67258794 MS.gene070538:CDS 30.0%
TGTAGTATTTGTAAACCCTA+GGG - chr5.4:67258322-67258341 None:intergenic 30.0%
TTAATAACCGATCATTTCCA+AGG + chr5.4:67259098-67259117 MS.gene070538:CDS 30.0%
! GTGAATGTGATTTTTCTGTT+GGG - chr5.4:67258377-67258396 None:intergenic 30.0%
!! CTTTGATTTTCTTGAAGCTT+GGG + chr5.4:67258445-67258464 MS.gene070538:CDS 30.0%
!! GATAAAAAATGAATGGACTG+AGG + chr5.4:67258733-67258752 MS.gene070538:CDS 30.0%
!! TCTTTGATTTTCTTGAAGCT+TGG + chr5.4:67258444-67258463 MS.gene070538:CDS 30.0%
!! TTTGATTTTCTTGAAGCTTG+GGG + chr5.4:67258446-67258465 MS.gene070538:CDS 30.0%
AATGCTTCAGATTCTATCCA+AGG + chr5.4:67259163-67259182 MS.gene070538:CDS 35.0%
AGGAGATTCTGTAGAATTTG+AGG - chr5.4:67258549-67258568 None:intergenic 35.0%
ATACAAGATTGAGCTGATCA+AGG - chr5.4:67259369-67259388 None:intergenic 35.0%
CAAAATTGATAAGCTTGTCC+TGG - chr5.4:67259282-67259301 None:intergenic 35.0%
CGTCGTATTATTCTTATCAC+AGG - chr5.4:67258630-67258649 None:intergenic 35.0%
CTGTAGTATTTGTAAACCCT+AGG - chr5.4:67258323-67258342 None:intergenic 35.0%
TCTTTATCTTCCCATATCAG+AGG - chr5.4:67258509-67258528 None:intergenic 35.0%
TGCAAATTTACAGCTTGAGA+AGG + chr5.4:67259198-67259217 MS.gene070538:CDS 35.0%
TTACAAATTTCGGGGATGAT+TGG + chr5.4:67258783-67258802 MS.gene070538:CDS 35.0%
! CGTGAATGTGATTTTTCTGT+TGG - chr5.4:67258378-67258397 None:intergenic 35.0%
! GGACAAGCTTATCAATTTTG+AGG + chr5.4:67259282-67259301 MS.gene070538:CDS 35.0%
AATGTGTCTCACACCTCTTA+TGG + chr5.4:67258952-67258971 MS.gene070538:CDS 40.0%
ACGGATTTCACATTCGTGAA+TGG - chr5.4:67258276-67258295 None:intergenic 40.0%
ATTCTCTTAGCTTCATTGGC+AGG - chr5.4:67258581-67258600 None:intergenic 40.0%
GATGTTCGCAAAATCCTAGT+CGG + chr5.4:67258398-67258417 MS.gene070538:CDS 40.0%
GATTCACATTCAGCATCAAC+GGG - chr5.4:67258899-67258918 None:intergenic 40.0%
GCAGATTCTCTTAGCTTCAT+TGG - chr5.4:67258585-67258604 None:intergenic 40.0%
GCTCAGTAAACCTCTGATAT+GGG + chr5.4:67258496-67258515 MS.gene070538:CDS 40.0%
GGATTCACATTCAGCATCAA+CGG - chr5.4:67258900-67258919 None:intergenic 40.0%
TCTTGCTGCAATGTATTCCT+TGG - chr5.4:67259118-67259137 None:intergenic 40.0%
TGGAAGATTCGGGAATGTTA+CGG - chr5.4:67258295-67258314 None:intergenic 40.0%
TTCTACAGAATCTCCTTCTC+TGG + chr5.4:67258553-67258572 MS.gene070538:CDS 40.0%
TTTACCAGACGCAACAGTTT+CGG - chr5.4:67258921-67258940 None:intergenic 40.0%
TTTGTAAACCCTAGGGTTCT+TGG - chr5.4:67258315-67258334 None:intergenic 40.0%
! ATCTGAAGCATTACCACCAT+CGG - chr5.4:67259155-67259174 None:intergenic 40.0%
! TTGCTCATCAAGTTGTTGGT+AGG + chr5.4:67258813-67258832 MS.gene070538:CDS 40.0%
!! CGTGGAAAAAGCTATTTCTG+AGG + chr5.4:67259231-67259250 MS.gene070538:CDS 40.0%
!!! AGGTTTTGGGTTCTGGTAAT+GGG - chr5.4:67258196-67258215 None:intergenic 40.0%
!!! CGTTTTCTAGAGCTTCCTTT+TGG + chr5.4:67258857-67258876 MS.gene070538:CDS 40.0%
AAAACAACTGCACCAACGAC+CGG + chr5.4:67259394-67259413 MS.gene070538:CDS 45.0%
AAAATCCTAGTCGGTGACGT+TGG + chr5.4:67258407-67258426 MS.gene070538:CDS 45.0%
ACGAGCAACACATTCCTTGT+CGG - chr5.4:67258840-67258859 None:intergenic 45.0%
AGAACCATCTTGAGTGGCTA+GGG - chr5.4:67258244-67258263 None:intergenic 45.0%
ATAATACGACGTTGTGTGCG+AGG + chr5.4:67258639-67258658 MS.gene070538:CDS 45.0%
ATTCGTGGGAGCTATCGAAT+TGG + chr5.4:67258668-67258687 MS.gene070538:CDS 45.0%
ATTTCGGGGATGATTGGAAG+AGG + chr5.4:67258789-67258808 MS.gene070538:CDS 45.0%
CAAGAGAGTGGAGATAAGTG+AGG + chr5.4:67258709-67258728 MS.gene070538:CDS 45.0%
CCACTCAAGATGGTTCTCAT+GGG + chr5.4:67258247-67258266 MS.gene070538:CDS 45.0%
CCTAGGGTTCTTGGAAGATT+CGG - chr5.4:67258306-67258325 None:intergenic 45.0%
CGAATCTTCCAAGAACCCTA+GGG + chr5.4:67258304-67258323 MS.gene070538:CDS 45.0%
CGCTCAGTAAACCTCTGATA+TGG + chr5.4:67258495-67258514 MS.gene070538:CDS 45.0%
CTAGGGTTCTTGGAAGATTC+GGG - chr5.4:67258305-67258324 None:intergenic 45.0%
CTTTCCTTTCTGCTTTGGCA+GGG + chr5.4:67259005-67259024 MS.gene070538:CDS 45.0%
GCTAGGGACAAGGATTATGT+TGG - chr5.4:67258228-67258247 None:intergenic 45.0%
GGATGCTAGCAATCCTATCA+TGG + chr5.4:67258973-67258992 MS.gene070538:CDS 45.0%
GGATTGCTAGCATCCATAAG+AGG - chr5.4:67258968-67258987 None:intergenic 45.0%
GGTTTACTGAGCGAAGAAGA+AGG - chr5.4:67258488-67258507 None:intergenic 45.0%
TAGCAATCCTATCATGGCTC+AGG + chr5.4:67258979-67258998 MS.gene070538:CDS 45.0%
TGAGAAGGAAGAATCAGACG+TGG + chr5.4:67259213-67259232 MS.gene070538:CDS 45.0%
TGAGGATTTAGACGTGCTGA+TGG + chr5.4:67259300-67259319 MS.gene070538:CDS 45.0%
TTACCAGAACCCAAAACCTC+TGG + chr5.4:67258197-67258216 MS.gene070538:CDS 45.0%
TTGCACGCAATAGAGATCCT+TGG - chr5.4:67259183-67259202 None:intergenic 45.0%
TTTCGGGGATGATTGGAAGA+GGG + chr5.4:67258790-67258809 MS.gene070538:CDS 45.0%
! AGGGTTGCTCATCAAGTTGT+TGG + chr5.4:67258809-67258828 MS.gene070538:CDS 45.0%
!! GTGCGAGGTGTTTTATTCGT+GGG + chr5.4:67258654-67258673 MS.gene070538:CDS 45.0%
!! TGTGCGAGGTGTTTTATTCG+TGG + chr5.4:67258653-67258672 MS.gene070538:CDS 45.0%
!!! GAGGTTTTGGGTTCTGGTAA+TGG - chr5.4:67258197-67258216 None:intergenic 45.0%
AAAGCAGCCTGAGCCATGAT+AGG - chr5.4:67258989-67259008 None:intergenic 50.0%
ATCTTGAGTGGCTAGGGACA+AGG - chr5.4:67258238-67258257 None:intergenic 50.0%
CCCATGAGAACCATCTTGAG+TGG - chr5.4:67258250-67258269 None:intergenic 50.0%
CCGAATCTTCCAAGAACCCT+AGG + chr5.4:67258303-67258322 MS.gene070538:CDS 50.0%
GAATCCGAAACTGTTGCGTC+TGG + chr5.4:67258914-67258933 MS.gene070538:CDS 50.0%
GACGGTTCTATTTGGCGTTG+AGG - chr5.4:67258111-67258130 None:intergenic 50.0%
GAGAACCATCTTGAGTGGCT+AGG - chr5.4:67258245-67258264 None:intergenic 50.0%
GAGAATCTGCAGTGTCTGCA+TGG + chr5.4:67258595-67258614 MS.gene070538:CDS 50.0%
GATGCTTCTGTTGCATCCGA+TGG + chr5.4:67259136-67259155 MS.gene070538:CDS 50.0%
GCCACTCAAGATGGTTCTCA+TGG + chr5.4:67258246-67258265 MS.gene070538:CDS 50.0%
GCTTTCCTTTCTGCTTTGGC+AGG + chr5.4:67259004-67259023 MS.gene070538:CDS 50.0%
GGAAAAAGAACGCCAGCAGT+TGG + chr5.4:67259321-67259340 MS.gene070538:CDS 50.0%
GGCTTCAACTGATCGCCAAA+AGG - chr5.4:67258875-67258894 None:intergenic 50.0%
TCACATTCAGCATCAACGGG+AGG - chr5.4:67258896-67258915 None:intergenic 50.0%
TCTTCGTTTGCTCCAACTGC+TGG - chr5.4:67259336-67259355 None:intergenic 50.0%
TTGTCCCTAGCCACTCAAGA+TGG + chr5.4:67258237-67258256 MS.gene070538:CDS 50.0%
! AGTTGTTGGTAGGACCGACA+AGG + chr5.4:67258823-67258842 MS.gene070538:CDS 50.0%
! GGCTGCTTTCCTTTCTGCTT+TGG + chr5.4:67259000-67259019 MS.gene070538:CDS 50.0%
!! GGTTCTGGTAATGGGACTTC+CGG - chr5.4:67258188-67258207 None:intergenic 50.0%
AGTGTTTCGACGGCGGAAGA+CGG - chr5.4:67258140-67258159 None:intergenic 55.0%
CCTTTCTGCTTTGGCAGGGA+CGG + chr5.4:67259009-67259028 MS.gene070538:CDS 55.0%
CGGTGGTTGCAGTGTTTCGA+CGG - chr5.4:67258150-67258169 None:intergenic 55.0%
GATTATGTTGGCGTCGCCAG+AGG - chr5.4:67258216-67258235 None:intergenic 55.0%
GCTTCTGTTGCATCCGATGG+TGG + chr5.4:67259139-67259158 MS.gene070538:CDS 55.0%
GGAGGCTCGACGGTTCTATT+TGG - chr5.4:67258119-67258138 None:intergenic 55.0%
TCATTGGCAGGCACCAGAGA+AGG - chr5.4:67258569-67258588 None:intergenic 55.0%
TGGTTGCAGTGTTTCGACGG+CGG - chr5.4:67258147-67258166 None:intergenic 55.0%
! TGGAACCAACGTCACCGACT+AGG - chr5.4:67258415-67258434 None:intergenic 55.0%
! TTGGCGTCGCCAGAGGTTTT+GGG - chr5.4:67258209-67258228 None:intergenic 55.0%
!!! TCGCCAGAGGTTTTGGGTTC+TGG - chr5.4:67258203-67258222 None:intergenic 55.0%
AACACTGCAACCACCGCCTC+CGG + chr5.4:67258154-67258173 MS.gene070538:CDS 60.0%
CCGTCCCTGCCAAAGCAGAA+AGG - chr5.4:67259012-67259031 None:intergenic 60.0%
TTCTGCTTTGGCAGGGACGG+AGG + chr5.4:67259012-67259031 MS.gene070538:CDS 60.0%
! GTTGGCGTCGCCAGAGGTTT+TGG - chr5.4:67258210-67258229 None:intergenic 60.0%
ACTTCCGGTTTCGCCACCGG+AGG - chr5.4:67258173-67258192 None:intergenic 65.0%
GGGACTTCCGGTTTCGCCAC+CGG - chr5.4:67258176-67258195 None:intergenic 65.0%
GTTTCGACGGCGGAAGACGG+AGG - chr5.4:67258137-67258156 None:intergenic 65.0%
ACCGCCTCCGGTGGCGAAAC+CGG + chr5.4:67258166-67258185 MS.gene070538:CDS 70.0%
ACTGCAACCACCGCCTCCGG+TGG + chr5.4:67258157-67258176 MS.gene070538:CDS 70.0%
GGCGGAAGACGGAGGCTCGA+CGG - chr5.4:67258129-67258148 None:intergenic 70.0%
TCCGGTTTCGCCACCGGAGG+CGG - chr5.4:67258170-67258189 None:intergenic 70.0%
GGTTTCGCCACCGGAGGCGG+TGG - chr5.4:67258167-67258186 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 67258101 67259423 67258101 ID=MS.gene070538
chr5.4 mRNA 67258101 67259423 67258101 ID=MS.gene070538.t1;Parent=MS.gene070538
chr5.4 exon 67258101 67259423 67258101 ID=MS.gene070538.t1.exon1;Parent=MS.gene070538.t1
chr5.4 CDS 67258101 67259423 67258101 ID=cds.MS.gene070538.t1;Parent=MS.gene070538.t1
Gene Sequence

>MS.gene070538

ATGGCGACCTCAACGCCAAATAGAACCGTCGAGCCTCCGTCTTCCGCCGTCGAAACACTGCAACCACCGCCTCCGGTGGCGAAACCGGAAGTCCCATTACCAGAACCCAAAACCTCTGGCGACGCCAACATAATCCTTGTCCCTAGCCACTCAAGATGGTTCTCATGGGATTCCATTCACGAATGTGAAATCCGTAACATTCCCGAATCTTCCAAGAACCCTAGGGTTTACAAATACTACAGAAACTCTATCGTCAAGTTTTTCAGATTCAATCCCAACAGAAAAATCACATTCACGGATGTTCGCAAAATCCTAGTCGGTGACGTTGGTTCCATTCGAAGAGTCTTTGATTTTCTTGAAGCTTGGGGTTTAATCAATTATCATCCTTCTTCTTCGCTCAGTAAACCTCTGATATGGGAAGATAAAGATGCTAAACTCGACGCAGCCTCAAATTCTACAGAATCTCCTTCTCTGGTGCCTGCCAATGAAGCTAAGAGAATCTGCAGTGTCTGCATGGCTTGCTTTGCCTGTGATAAGAATAATACGACGTTGTGTGCGAGGTGTTTTATTCGTGGGAGCTATCGAATTGGTATGAGTAATACAGAATTCAAGAGAGTGGAGATAAGTGAGGAGATAAAAAATGAATGGACTGAGGAAGAAACACTGAATCTTCTTGAAGCTATTACAAATTTCGGGGATGATTGGAAGAGGGTTGCTCATCAAGTTGTTGGTAGGACCGACAAGGAATGTGTTGCTCGTTTTCTAGAGCTTCCTTTTGGCGATCAGTTGAAGCCTCCCGTTGATGCTGAATGTGAATCCGAAACTGTTGCGTCTGGTAAATCTAGTAAGAGAATGTGTCTCACACCTCTTATGGATGCTAGCAATCCTATCATGGCTCAGGCTGCTTTCCTTTCTGCTTTGGCAGGGACGGAGGTTGCACAAGCTGCAGCTCAAGCTGCTCTGACAAGTTTGTCCAATGTTTACAAATCAAGTAGAATTAATAACCGATCATTTCCAAGGAATACATTGCAGCAAGATGCTTCTGTTGCATCCGATGGTGGTAATGCTTCAGATTCTATCCAAGGATCTCTATTGCGTGCAAATTTACAGCTTGAGAAGGAAGAATCAGACGTGGAAAAAGCTATTTCTGAGGTTATAGAAGTTCAGATGAAAAATATCCAGGACAAGCTTATCAATTTTGAGGATTTAGACGTGCTGATGGAAAAAGAACGCCAGCAGTTGGAGCAAACGAAGAATTTGATTTTCCTTGATCAGCTCAATCTTGTATTTCGTAAAACAACTGCACCAACGACCGGAAAGTAA
Protein sequence

>MS.gene070538.t1

MATSTPNRTVEPPSSAVETLQPPPPVAKPEVPLPEPKTSGDANIILVPSHSRWFSWDSIHECEIRNIPESSKNPRVYKYYRNSIVKFFRFNPNRKITFTDVRKILVGDVGSIRRVFDFLEAWGLINYHPSSSLSKPLIWEDKDAKLDAASNSTESPSLVPANEAKRICSVCMACFACDKNNTTLCARCFIRGSYRIGMSNTEFKRVEISEEIKNEWTEEETLNLLEAITNFGDDWKRVAHQVVGRTDKECVARFLELPFGDQLKPPVDAECESETVASGKSSKRMCLTPLMDASNPIMAQAAFLSALAGTEVAQAAAQAALTSLSNVYKSSRINNRSFPRNTLQQDASVASDGGNASDSIQGSLLRANLQLEKEESDVEKAISEVIEVQMKNIQDKLINFEDLDVLMEKERQQLEQTKNLIFLDQLNLVFRKTTAPTTGK