AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene016712

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016712.t1 MTR_5g085170 89.091 440 34 1 1 440 1 426 0.0 780
MS.gene016712.t1 MTR_3g116120 84.244 476 39 3 1 440 1 476 0.0 772
MS.gene016712.t1 MTR_6g053260 77.091 275 18 4 1 275 1 230 1.47e-127 385
MS.gene016712.t1 MTR_6g053260 91.579 95 8 0 344 438 234 328 1.63e-47 174
MS.gene016712.t1 MTR_3g099000 33.916 286 125 7 44 267 15 298 9.52e-39 147
MS.gene016712.t1 MTR_4g069830 26.689 296 158 9 39 280 133 423 1.29e-22 101
MS.gene016712.t1 MTR_4g069830 26.689 296 158 9 39 280 133 423 1.30e-22 101
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016712.t1 AT2G33610 50.350 429 179 8 38 438 41 463 4.68e-134 394
MS.gene016712.t1 AT1G21700 23.529 510 290 16 23 436 158 663 2.28e-36 142
MS.gene016712.t1 AT2G47620 28.455 492 247 16 39 429 7 494 8.48e-36 138
MS.gene016712.t1 AT2G47620 34.559 272 128 9 39 263 7 275 4.94e-32 126
MS.gene016712.t1 AT4G34430 29.893 281 146 8 39 268 139 419 1.08e-21 98.6
MS.gene016712.t1 AT4G34430 29.893 281 146 8 39 268 139 419 1.09e-21 98.6
MS.gene016712.t1 AT4G34430 29.893 281 146 8 39 268 139 419 1.09e-21 98.6
MS.gene016712.t1 AT4G34430 29.893 281 146 8 39 268 139 419 1.16e-21 98.6

Find 97 sgRNAs with CRISPR-Local

Find 115 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAAGCTATTACAAATTTC+GGG 0.188443 5.3:+68783383 MS.gene016712:CDS
CTTGAAGCTATTACAAATTT+CGG 0.191024 5.3:+68783382 MS.gene016712:CDS
CGCTCAGTAAACCTCTGATA+TGG 0.237129 5.3:+68783104 MS.gene016712:CDS
CGTTCTGGTAATGGGACTTC+TGG 0.306086 5.3:-68782794 None:intergenic
GGCTGCTTTCCTTTCTGCTT+TGG 0.311179 5.3:+68783609 MS.gene016712:CDS
TTTGTAAACCCTAGGGTTCT+TGG 0.330782 5.3:-68782921 None:intergenic
GTCAGTCATCAATTACTTTC+CGG 0.330900 5.3:-68784022 None:intergenic
GCTTTCCTTTCTGCTTTGGC+AGG 0.337459 5.3:+68783613 MS.gene016712:CDS
CCTAGGGTTCTTGGAAGATT+CGG 0.357797 5.3:-68782912 None:intergenic
GGAGGCTCGACGGTTCTATT+TGG 0.364466 5.3:-68782725 None:intergenic
CTAGGGTTCTTGGAAGATTC+GGG 0.380857 5.3:-68782911 None:intergenic
AATGTGTCTCACACCTCTTA+TGG 0.384773 5.3:+68783561 MS.gene016712:CDS
TTTACCAGACGCAACAGTTT+CGG 0.399478 5.3:-68783527 None:intergenic
TCTTCGTTTGCTCCAACTGC+TGG 0.408427 5.3:-68783942 None:intergenic
ATCAATTACTTTCCGGTCGT+TGG 0.415852 5.3:-68784015 None:intergenic
TTACAAATTTCGGGGATGAT+TGG 0.415906 5.3:+68783392 MS.gene016712:CDS
GCAGATTCTCTTAGCTTCAT+TGG 0.416279 5.3:-68783191 None:intergenic
CCTTTCTGCTTTGGCAGGGA+CGG 0.421374 5.3:+68783618 MS.gene016712:CDS
TCTGGTTTCGCCACCGGAGG+TGG 0.425880 5.3:-68782776 None:intergenic
ACGGATTTCGCATTCGTGAA+TGG 0.432570 5.3:-68782882 None:intergenic
AGGAGATTCTGTAGAATTTG+AGG 0.449273 5.3:-68783155 None:intergenic
TCTTGCTGCAATGTATTCCT+TGG 0.449690 5.3:-68783724 None:intergenic
TTCTACAGAATCTCCTTCTC+TGG 0.459502 5.3:+68783162 MS.gene016712:CDS
GGTTTCGCCACCGGAGGTGG+TGG 0.461023 5.3:-68782773 None:intergenic
AGGGTTGCTCATCAAGTTGT+TGG 0.467288 5.3:+68783418 MS.gene016712:CDS
ATTCTCTTAGCTTCATTGGC+AGG 0.470496 5.3:-68783187 None:intergenic
TCTACTTGATTTGTAAACAT+TGG 0.471603 5.3:-68783683 None:intergenic
GGGACTTCTGGTTTCGCCAC+CGG 0.473017 5.3:-68782782 None:intergenic
AAAGCAGCCTGAGCCATGAT+AGG 0.474193 5.3:-68783595 None:intergenic
GATTCACATTCAGCATCAAC+GGG 0.474269 5.3:-68783505 None:intergenic
AACACTGCAACCACCACCTC+CGG 0.479112 5.3:+68782763 MS.gene016712:CDS
CAAAATTGATAAGCTTGTCC+TGG 0.486513 5.3:-68783888 None:intergenic
CTGTAGTATTTGTAAACCCT+AGG 0.490342 5.3:-68782929 None:intergenic
GCTCAGTAAACCTCTGATAT+GGG 0.498668 5.3:+68783105 MS.gene016712:CDS
CCGAATCTTCCAAGAACCCT+AGG 0.500165 5.3:+68782912 MS.gene016712:CDS
GCCACTCAAGATGGTTCTCA+TGG 0.500382 5.3:+68782855 MS.gene016712:CDS
TGAGGATTTAGACGTGGTGA+TGG 0.502057 5.3:+68783909 MS.gene016712:CDS
TTGCACGCAATAGAGATCCT+TGG 0.503227 5.3:-68783789 None:intergenic
GGATGCTAGCAATCCTATCA+TGG 0.508578 5.3:+68783582 MS.gene016712:CDS
AAAACAACTGCACCAACGAC+CGG 0.508881 5.3:+68784003 MS.gene016712:CDS
CTTTCCTTTCTGCTTTGGCA+GGG 0.515547 5.3:+68783614 MS.gene016712:CDS
ATTCGTGGGAACTATAGGAT+TGG 0.516233 5.3:+68783277 MS.gene016712:CDS
TTACCAGAACGCAAAACCTC+TGG 0.520797 5.3:+68782806 MS.gene016712:CDS
TGGTGGTTGCAGTGTTTCAA+CGG 0.522006 5.3:-68782756 None:intergenic
TAGCAATCCTATCATGGCTC+AGG 0.529443 5.3:+68783588 MS.gene016712:CDS
GGATTGCTAGCATCCATAAG+AGG 0.529525 5.3:-68783574 None:intergenic
ACGAGCAACACATTCCTTGT+CGG 0.529759 5.3:-68783446 None:intergenic
GGCTTCAACTGATCGCCAAA+AGG 0.531669 5.3:-68783481 None:intergenic
CGTCGTATTGTTCTTATCAC+AGG 0.533035 5.3:-68783236 None:intergenic
TGTAGTATTTGTAAACCCTA+GGG 0.534357 5.3:-68782928 None:intergenic
AGAACCATCTTGAGTGGCTA+GGG 0.535119 5.3:-68782850 None:intergenic
GGATTCACATTCAGCATCAA+CGG 0.539105 5.3:-68783506 None:intergenic
ATACAAGATTGAGCTGATCA+AGG 0.541570 5.3:-68783975 None:intergenic
ATCTGAAGCATTACCACCAT+CGG 0.545048 5.3:-68783761 None:intergenic
ATGTATTCCTTGGAAATGAT+CGG 0.546642 5.3:-68783714 None:intergenic
GATGCTTCTGTTGCATCCGA+TGG 0.547872 5.3:+68783745 MS.gene016712:CDS
GGTTTACTGAGCGAAGAAGA+AGG 0.548651 5.3:-68783094 None:intergenic
ATTTCGGGGATGATTGGAAG+AGG 0.548838 5.3:+68783398 MS.gene016712:CDS
ATCTTGAGTGGCTAGGGACA+AGG 0.549549 5.3:-68782844 None:intergenic
TTCTGCTTTGGCAGGGACGG+AGG 0.550159 5.3:+68783621 MS.gene016712:CDS
TTAATAACCGATCATTTCCA+AGG 0.554025 5.3:+68783707 MS.gene016712:CDS
CGAATCTTCCAAGAACCCTA+GGG 0.558609 5.3:+68782913 MS.gene016712:CDS
AAAATCAAAGACTCTTCGAA+TGG 0.560578 5.3:-68783041 None:intergenic
TCATTGGCAGGCACCAGAGA+AGG 0.560713 5.3:-68783175 None:intergenic
TGCAAATTTACAGCTTGCGA+AGG 0.562614 5.3:+68783807 MS.gene016712:CDS
TTGCTCATCAAGTTGTTGGT+AGG 0.563248 5.3:+68783422 MS.gene016712:CDS
CCGTCCCTGCCAAAGCAGAA+AGG 0.564962 5.3:-68783618 None:intergenic
ACTTCTGGTTTCGCCACCGG+AGG 0.565320 5.3:-68782779 None:intergenic
TGGAACCAACGTCACCAACT+AGG 0.567588 5.3:-68783021 None:intergenic
TGGAAGATTCGGGAATGTAA+CGG 0.569089 5.3:-68782901 None:intergenic
TAATACAGAATTCAAGAGAG+TGG 0.570887 5.3:+68783306 MS.gene016712:CDS
ACAATACGACGTTGTGTGCG+AGG 0.571252 5.3:+68783248 MS.gene016712:CDS
GATGTTCGCAAAATCCTAGT+TGG 0.572423 5.3:+68783007 MS.gene016712:CDS
GAGAACCATCTTGAGTGGCT+AGG 0.580360 5.3:-68782851 None:intergenic
GAATCCGAAACTGTTGCGTC+TGG 0.583173 5.3:+68783523 MS.gene016712:CDS
AGTGTTTCAACGGCGGAAGA+CGG 0.585442 5.3:-68782746 None:intergenic
AATGCTTCAGATTCTATCCA+AGG 0.586687 5.3:+68783772 MS.gene016712:CDS
GAGAATCTGCAGTGTCTGCA+TGG 0.586827 5.3:+68783204 MS.gene016712:CDS
TTGTCCCTAGCCACTCAAGA+TGG 0.586862 5.3:+68782846 MS.gene016712:CDS
TCTTTATCTTCCCATATCAG+AGG 0.587798 5.3:-68783115 None:intergenic
CAAGAGAGTGGAGATAAGTG+AGG 0.590112 5.3:+68783318 MS.gene016712:CDS
GACGGTTCTATTTGGCGTTG+AGG 0.590322 5.3:-68782717 None:intergenic
GATTATGTTGGCGTCGCCAG+AGG 0.591406 5.3:-68782822 None:intergenic
GGCGGAAGACGGAGGCTCGA+CGG 0.592570 5.3:-68782735 None:intergenic
GCTAGGGACAAGGATTATGT+TGG 0.592687 5.3:-68782834 None:intergenic
TTTCGGGGATGATTGGAAGA+GGG 0.613337 5.3:+68783399 MS.gene016712:CDS
ACTGCAACCACCACCTCCGG+TGG 0.615979 5.3:+68782766 MS.gene016712:CDS
TGAAGCTATTACAAATTTCG+GGG 0.621595 5.3:+68783384 MS.gene016712:CDS
CCACTCAAGATGGTTCTCAT+GGG 0.624877 5.3:+68782856 MS.gene016712:CDS
AAAATCCTAGTTGGTGACGT+TGG 0.629448 5.3:+68783016 MS.gene016712:CDS
GCTTCTGTTGCATCCGATGG+TGG 0.643243 5.3:+68783748 MS.gene016712:CDS
CCCATGAGAACCATCTTGAG+TGG 0.645166 5.3:-68782856 None:intergenic
AGTTGTTGGTAGGACCGACA+AGG 0.658858 5.3:+68783432 MS.gene016712:CDS
TCACATTCAGCATCAACGGG+AGG 0.659217 5.3:-68783502 None:intergenic
GTTTCAACGGCGGAAGACGG+AGG 0.661965 5.3:-68782743 None:intergenic
TGCGAAGGAAGAATCAGACG+TGG 0.692021 5.3:+68783822 MS.gene016712:CDS
TGGTTGCAGTGTTTCAACGG+CGG 0.699529 5.3:-68782753 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CTTGAAGCTATTACAAATTT+CGG + chr5.3:68783382-68783401 MS.gene016712:CDS 25.0%
! TTGAAGCTATTACAAATTTC+GGG + chr5.3:68783383-68783402 MS.gene016712:CDS 25.0%
!!! TCTACTTGATTTGTAAACAT+TGG - chr5.3:68783686-68783705 None:intergenic 25.0%
AAAATCAAAGACTCTTCGAA+TGG - chr5.3:68783044-68783063 None:intergenic 30.0%
AGTTCAGATGAAAAATATCC+AGG + chr5.3:68783870-68783889 MS.gene016712:CDS 30.0%
ATGTATTCCTTGGAAATGAT+CGG - chr5.3:68783717-68783736 None:intergenic 30.0%
CAACAGAAAAATCACATTCA+CGG + chr5.3:68782985-68783004 MS.gene016712:CDS 30.0%
TAATACAGAATTCAAGAGAG+TGG + chr5.3:68783306-68783325 MS.gene016712:CDS 30.0%
TGAAGCTATTACAAATTTCG+GGG + chr5.3:68783384-68783403 MS.gene016712:CDS 30.0%
TGTAGTATTTGTAAACCCTA+GGG - chr5.3:68782931-68782950 None:intergenic 30.0%
TTAATAACCGATCATTTCCA+AGG + chr5.3:68783707-68783726 MS.gene016712:CDS 30.0%
! GTGAATGTGATTTTTCTGTT+GGG - chr5.3:68782986-68783005 None:intergenic 30.0%
!! CTTTGATTTTCTTGAAGCTT+GGG + chr5.3:68783054-68783073 MS.gene016712:CDS 30.0%
!! TCTTTGATTTTCTTGAAGCT+TGG + chr5.3:68783053-68783072 MS.gene016712:CDS 30.0%
!! TTTGATTTTCTTGAAGCTTG+GGG + chr5.3:68783055-68783074 MS.gene016712:CDS 30.0%
AATGCTTCAGATTCTATCCA+AGG + chr5.3:68783772-68783791 MS.gene016712:CDS 35.0%
AGGAGATTCTGTAGAATTTG+AGG - chr5.3:68783158-68783177 None:intergenic 35.0%
ATACAAGATTGAGCTGATCA+AGG - chr5.3:68783978-68783997 None:intergenic 35.0%
CAAAATTGATAAGCTTGTCC+TGG - chr5.3:68783891-68783910 None:intergenic 35.0%
CTGTAGTATTTGTAAACCCT+AGG - chr5.3:68782932-68782951 None:intergenic 35.0%
GTGAGGAGACAAAAAATGAA+TGG + chr5.3:68783335-68783354 MS.gene016712:CDS 35.0%
TCTTTATCTTCCCATATCAG+AGG - chr5.3:68783118-68783137 None:intergenic 35.0%
TTACAAATTTCGGGGATGAT+TGG + chr5.3:68783392-68783411 MS.gene016712:CDS 35.0%
! CGTGAATGTGATTTTTCTGT+TGG - chr5.3:68782987-68783006 None:intergenic 35.0%
! GGACAAGCTTATCAATTTTG+AGG + chr5.3:68783891-68783910 MS.gene016712:CDS 35.0%
!! CAATTTTGAGGATTTAGACG+TGG + chr5.3:68783903-68783922 MS.gene016712:CDS 35.0%
!! GACAAAAAATGAATGGACTG+AGG + chr5.3:68783342-68783361 MS.gene016712:CDS 35.0%
!! GTTTTATTCGTGGGAACTAT+AGG + chr5.3:68783272-68783291 MS.gene016712:CDS 35.0%
AATGTGTCTCACACCTCTTA+TGG + chr5.3:68783561-68783580 MS.gene016712:CDS 40.0%
ATTCGTGGGAACTATAGGAT+TGG + chr5.3:68783277-68783296 MS.gene016712:CDS 40.0%
ATTCTCTTAGCTTCATTGGC+AGG - chr5.3:68783190-68783209 None:intergenic 40.0%
CGTCGTATTGTTCTTATCAC+AGG - chr5.3:68783239-68783258 None:intergenic 40.0%
GATGTTCGCAAAATCCTAGT+TGG + chr5.3:68783007-68783026 MS.gene016712:CDS 40.0%
GATTCACATTCAGCATCAAC+GGG - chr5.3:68783508-68783527 None:intergenic 40.0%
GCAGATTCTCTTAGCTTCAT+TGG - chr5.3:68783194-68783213 None:intergenic 40.0%
GCTCAGTAAACCTCTGATAT+GGG + chr5.3:68783105-68783124 MS.gene016712:CDS 40.0%
GGATTCACATTCAGCATCAA+CGG - chr5.3:68783509-68783528 None:intergenic 40.0%
TCTTGCTGCAATGTATTCCT+TGG - chr5.3:68783727-68783746 None:intergenic 40.0%
TGCAAATTTACAGCTTGCGA+AGG + chr5.3:68783807-68783826 MS.gene016712:CDS 40.0%
TGGAAGATTCGGGAATGTAA+CGG - chr5.3:68782904-68782923 None:intergenic 40.0%
TTCTACAGAATCTCCTTCTC+TGG + chr5.3:68783162-68783181 MS.gene016712:CDS 40.0%
TTTACCAGACGCAACAGTTT+CGG - chr5.3:68783530-68783549 None:intergenic 40.0%
TTTGTAAACCCTAGGGTTCT+TGG - chr5.3:68782924-68782943 None:intergenic 40.0%
! AGGTTTTGCGTTCTGGTAAT+GGG - chr5.3:68782805-68782824 None:intergenic 40.0%
! ATCTGAAGCATTACCACCAT+CGG - chr5.3:68783764-68783783 None:intergenic 40.0%
! TTGCTCATCAAGTTGTTGGT+AGG + chr5.3:68783422-68783441 MS.gene016712:CDS 40.0%
!! AAAATCCTAGTTGGTGACGT+TGG + chr5.3:68783016-68783035 MS.gene016712:CDS 40.0%
!! CGTGGAAAAAGCTATTTCTG+AGG + chr5.3:68783840-68783859 MS.gene016712:CDS 40.0%
!!! CGTTTTCTAGAGCTTCCTTT+TGG + chr5.3:68783466-68783485 MS.gene016712:CDS 40.0%
AAAACAACTGCACCAACGAC+CGG + chr5.3:68784003-68784022 MS.gene016712:CDS 45.0%
ACGAGCAACACATTCCTTGT+CGG - chr5.3:68783449-68783468 None:intergenic 45.0%
ACGGATTTCGCATTCGTGAA+TGG - chr5.3:68782885-68782904 None:intergenic 45.0%
AGAACCATCTTGAGTGGCTA+GGG - chr5.3:68782853-68782872 None:intergenic 45.0%
ATTTCGGGGATGATTGGAAG+AGG + chr5.3:68783398-68783417 MS.gene016712:CDS 45.0%
CAAGAGAGTGGAGATAAGTG+AGG + chr5.3:68783318-68783337 MS.gene016712:CDS 45.0%
CCACTCAAGATGGTTCTCAT+GGG + chr5.3:68782856-68782875 MS.gene016712:CDS 45.0%
CCTAGGGTTCTTGGAAGATT+CGG - chr5.3:68782915-68782934 None:intergenic 45.0%
CGAATCTTCCAAGAACCCTA+GGG + chr5.3:68782913-68782932 MS.gene016712:CDS 45.0%
CGCTCAGTAAACCTCTGATA+TGG + chr5.3:68783104-68783123 MS.gene016712:CDS 45.0%
CTAGGGTTCTTGGAAGATTC+GGG - chr5.3:68782914-68782933 None:intergenic 45.0%
CTTTCCTTTCTGCTTTGGCA+GGG + chr5.3:68783614-68783633 MS.gene016712:CDS 45.0%
GCTAGGGACAAGGATTATGT+TGG - chr5.3:68782837-68782856 None:intergenic 45.0%
GGATGCTAGCAATCCTATCA+TGG + chr5.3:68783582-68783601 MS.gene016712:CDS 45.0%
GGATTGCTAGCATCCATAAG+AGG - chr5.3:68783577-68783596 None:intergenic 45.0%
GGTTTACTGAGCGAAGAAGA+AGG - chr5.3:68783097-68783116 None:intergenic 45.0%
TAGCAATCCTATCATGGCTC+AGG + chr5.3:68783588-68783607 MS.gene016712:CDS 45.0%
TGAGGATTTAGACGTGGTGA+TGG + chr5.3:68783909-68783928 MS.gene016712:CDS 45.0%
TGGTGGTTGCAGTGTTTCAA+CGG - chr5.3:68782759-68782778 None:intergenic 45.0%
TTACCAGAACGCAAAACCTC+TGG + chr5.3:68782806-68782825 MS.gene016712:CDS 45.0%
TTGCACGCAATAGAGATCCT+TGG - chr5.3:68783792-68783811 None:intergenic 45.0%
TTTCGGGGATGATTGGAAGA+GGG + chr5.3:68783399-68783418 MS.gene016712:CDS 45.0%
! AGGGTTGCTCATCAAGTTGT+TGG + chr5.3:68783418-68783437 MS.gene016712:CDS 45.0%
! GAGGTTTTGCGTTCTGGTAA+TGG - chr5.3:68782806-68782825 None:intergenic 45.0%
!! GTGCGAGGTGTTTTATTCGT+GGG + chr5.3:68783263-68783282 MS.gene016712:CDS 45.0%
!! TGTGCGAGGTGTTTTATTCG+TGG + chr5.3:68783262-68783281 MS.gene016712:CDS 45.0%
AAAGCAGCCTGAGCCATGAT+AGG - chr5.3:68783598-68783617 None:intergenic 50.0%
ACAATACGACGTTGTGTGCG+AGG + chr5.3:68783248-68783267 MS.gene016712:CDS 50.0%
AGTGTTTCAACGGCGGAAGA+CGG - chr5.3:68782749-68782768 None:intergenic 50.0%
ATCTTGAGTGGCTAGGGACA+AGG - chr5.3:68782847-68782866 None:intergenic 50.0%
CCCATGAGAACCATCTTGAG+TGG - chr5.3:68782859-68782878 None:intergenic 50.0%
CCGAATCTTCCAAGAACCCT+AGG + chr5.3:68782912-68782931 MS.gene016712:CDS 50.0%
GAATCCGAAACTGTTGCGTC+TGG + chr5.3:68783523-68783542 MS.gene016712:CDS 50.0%
GACGGTTCTATTTGGCGTTG+AGG - chr5.3:68782720-68782739 None:intergenic 50.0%
GAGAACCATCTTGAGTGGCT+AGG - chr5.3:68782854-68782873 None:intergenic 50.0%
GAGAATCTGCAGTGTCTGCA+TGG + chr5.3:68783204-68783223 MS.gene016712:CDS 50.0%
GATGCTTCTGTTGCATCCGA+TGG + chr5.3:68783745-68783764 MS.gene016712:CDS 50.0%
GCCACTCAAGATGGTTCTCA+TGG + chr5.3:68782855-68782874 MS.gene016712:CDS 50.0%
GCTTTCCTTTCTGCTTTGGC+AGG + chr5.3:68783613-68783632 MS.gene016712:CDS 50.0%
GGAAAAAGAACGCCAGCAGT+TGG + chr5.3:68783930-68783949 MS.gene016712:CDS 50.0%
GGCTTCAACTGATCGCCAAA+AGG - chr5.3:68783484-68783503 None:intergenic 50.0%
TCACATTCAGCATCAACGGG+AGG - chr5.3:68783505-68783524 None:intergenic 50.0%
TCTTCGTTTGCTCCAACTGC+TGG - chr5.3:68783945-68783964 None:intergenic 50.0%
TGCGAAGGAAGAATCAGACG+TGG + chr5.3:68783822-68783841 MS.gene016712:CDS 50.0%
TGGAACCAACGTCACCAACT+AGG - chr5.3:68783024-68783043 None:intergenic 50.0%
TGGTTGCAGTGTTTCAACGG+CGG - chr5.3:68782756-68782775 None:intergenic 50.0%
TTGTCCCTAGCCACTCAAGA+TGG + chr5.3:68782846-68782865 MS.gene016712:CDS 50.0%
! AGTTGTTGGTAGGACCGACA+AGG + chr5.3:68783432-68783451 MS.gene016712:CDS 50.0%
! GGCTGCTTTCCTTTCTGCTT+TGG + chr5.3:68783609-68783628 MS.gene016712:CDS 50.0%
!! CGTTCTGGTAATGGGACTTC+TGG - chr5.3:68782797-68782816 None:intergenic 50.0%
AACACTGCAACCACCACCTC+CGG + chr5.3:68782763-68782782 MS.gene016712:CDS 55.0%
CCTTTCTGCTTTGGCAGGGA+CGG + chr5.3:68783618-68783637 MS.gene016712:CDS 55.0%
GATTATGTTGGCGTCGCCAG+AGG - chr5.3:68782825-68782844 None:intergenic 55.0%
GCTTCTGTTGCATCCGATGG+TGG + chr5.3:68783748-68783767 MS.gene016712:CDS 55.0%
GGAGGCTCGACGGTTCTATT+TGG - chr5.3:68782728-68782747 None:intergenic 55.0%
TCATTGGCAGGCACCAGAGA+AGG - chr5.3:68783178-68783197 None:intergenic 55.0%
! TCGCCAGAGGTTTTGCGTTC+TGG - chr5.3:68782812-68782831 None:intergenic 55.0%
CCGTCCCTGCCAAAGCAGAA+AGG - chr5.3:68783621-68783640 None:intergenic 60.0%
GTTTCAACGGCGGAAGACGG+AGG - chr5.3:68782746-68782765 None:intergenic 60.0%
TTCTGCTTTGGCAGGGACGG+AGG + chr5.3:68783621-68783640 MS.gene016712:CDS 60.0%
!! ACTTCTGGTTTCGCCACCGG+AGG - chr5.3:68782782-68782801 None:intergenic 60.0%
!! GGGACTTCTGGTTTCGCCAC+CGG - chr5.3:68782785-68782804 None:intergenic 60.0%
ACTGCAACCACCACCTCCGG+TGG + chr5.3:68782766-68782785 MS.gene016712:CDS 65.0%
! TCTGGTTTCGCCACCGGAGG+TGG - chr5.3:68782779-68782798 None:intergenic 65.0%
GGCGGAAGACGGAGGCTCGA+CGG - chr5.3:68782738-68782757 None:intergenic 70.0%
GGTTTCGCCACCGGAGGTGG+TGG - chr5.3:68782776-68782795 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 68782710 68784032 68782710 ID=MS.gene016712
chr5.3 mRNA 68782710 68784032 68782710 ID=MS.gene016712.t1;Parent=MS.gene016712
chr5.3 exon 68782710 68784032 68782710 ID=MS.gene016712.t1.exon1;Parent=MS.gene016712.t1
chr5.3 CDS 68782710 68784032 68782710 ID=cds.MS.gene016712.t1;Parent=MS.gene016712.t1
Gene Sequence

>MS.gene016712

ATGGCGACCTCAACGCCAAATAGAACCGTCGAGCCTCCGTCTTCCGCCGTTGAAACACTGCAACCACCACCTCCGGTGGCGAAACCAGAAGTCCCATTACCAGAACGCAAAACCTCTGGCGACGCCAACATAATCCTTGTCCCTAGCCACTCAAGATGGTTCTCATGGGATTCCATTCACGAATGCGAAATCCGTTACATTCCCGAATCTTCCAAGAACCCTAGGGTTTACAAATACTACAGAAACTCTATCGTCAAGTTTTTCAGATTCAATCCCAACAGAAAAATCACATTCACGGATGTTCGCAAAATCCTAGTTGGTGACGTTGGTTCCATTCGAAGAGTCTTTGATTTTCTTGAAGCTTGGGGTTTAATCAATTATGATCCTTCTTCTTCGCTCAGTAAACCTCTGATATGGGAAGATAAAGATGCTAAACTCGACGCAGCCTCAAATTCTACAGAATCTCCTTCTCTGGTGCCTGCCAATGAAGCTAAGAGAATCTGCAGTGTCTGCATGGCTTGCTTTGCCTGTGATAAGAACAATACGACGTTGTGTGCGAGGTGTTTTATTCGTGGGAACTATAGGATTGGTATGAGTAATACAGAATTCAAGAGAGTGGAGATAAGTGAGGAGACAAAAAATGAATGGACTGAGGAAGAAACACTGAATCTTCTTGAAGCTATTACAAATTTCGGGGATGATTGGAAGAGGGTTGCTCATCAAGTTGTTGGTAGGACCGACAAGGAATGTGTTGCTCGTTTTCTAGAGCTTCCTTTTGGCGATCAGTTGAAGCCTCCCGTTGATGCTGAATGTGAATCCGAAACTGTTGCGTCTGGTAAATCTAGTAAGAGAATGTGTCTCACACCTCTTATGGATGCTAGCAATCCTATCATGGCTCAGGCTGCTTTCCTTTCTGCTTTGGCAGGGACGGAGGTTGCACAAGCTGCAGCTCAAGCTGCTCTGACAAGTTTGTCCAATGTTTACAAATCAAGTAGAATTAATAACCGATCATTTCCAAGGAATACATTGCAGCAAGATGCTTCTGTTGCATCCGATGGTGGTAATGCTTCAGATTCTATCCAAGGATCTCTATTGCGTGCAAATTTACAGCTTGCGAAGGAAGAATCAGACGTGGAAAAAGCTATTTCTGAGGTTATAGAAGTTCAGATGAAAAATATCCAGGACAAGCTTATCAATTTTGAGGATTTAGACGTGGTGATGGAAAAAGAACGCCAGCAGTTGGAGCAAACGAAGAATTTGTTTTTCCTTGATCAGCTCAATCTTGTATTTCGTAAAACAACTGCACCAACGACCGGAAAGTAA
Protein sequence

>MS.gene016712.t1

MATSTPNRTVEPPSSAVETLQPPPPVAKPEVPLPERKTSGDANIILVPSHSRWFSWDSIHECEIRYIPESSKNPRVYKYYRNSIVKFFRFNPNRKITFTDVRKILVGDVGSIRRVFDFLEAWGLINYDPSSSLSKPLIWEDKDAKLDAASNSTESPSLVPANEAKRICSVCMACFACDKNNTTLCARCFIRGNYRIGMSNTEFKRVEISEETKNEWTEEETLNLLEAITNFGDDWKRVAHQVVGRTDKECVARFLELPFGDQLKPPVDAECESETVASGKSSKRMCLTPLMDASNPIMAQAAFLSALAGTEVAQAAAQAALTSLSNVYKSSRINNRSFPRNTLQQDASVASDGGNASDSIQGSLLRANLQLAKEESDVEKAISEVIEVQMKNIQDKLINFEDLDVVMEKERQQLEQTKNLFFLDQLNLVFRKTTAPTTGK