Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015411.t1 | XP_003611335.1 | 99.3 | 142 | 1 | 0 | 1 | 142 | 1 | 142 | 9.30E-73 | 282.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015411.t1 | Q9SKZ3 | 70.8 | 144 | 40 | 1 | 1 | 142 | 1 | 144 | 7.0e-53 | 208.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015411.t1 | G7JXD6 | 99.3 | 142 | 1 | 0 | 1 | 142 | 1 | 142 | 6.7e-73 | 282.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene015411 | MS.gene09779 | PPI |
| MS.gene013786 | MS.gene015411 | PPI |
| MS.gene015411 | MS.gene025830 | PPI |
| MS.gene02807 | MS.gene015411 | PPI |
| MS.gene57391 | MS.gene015411 | PPI |
| MS.gene09780 | MS.gene015411 | PPI |
| MS.gene46264 | MS.gene015411 | PPI |
| MS.gene030614 | MS.gene015411 | PPI |
| MS.gene015411 | MS.gene053443 | PPI |
| MS.gene09762 | MS.gene015411 | PPI |
| MS.gene015411 | MS.gene034249 | PPI |
| MS.gene015411 | MS.gene073888 | PPI |
| MS.gene015411 | MS.gene02572 | PPI |
| MS.gene65180 | MS.gene015411 | PPI |
| MS.gene015411 | MS.gene053224 | PPI |
| MS.gene03201 | MS.gene015411 | PPI |
| MS.gene70215 | MS.gene015411 | PPI |
| MS.gene53517 | MS.gene015411 | PPI |
| MS.gene28384 | MS.gene015411 | PPI |
| MS.gene015411 | MS.gene000610 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015411.t1 | MTR_5g012890 | 99.296 | 142 | 1 | 0 | 1 | 142 | 1 | 142 | 3.09e-101 | 286 |
| MS.gene015411.t1 | MTR_8g093770 | 94.444 | 126 | 7 | 0 | 17 | 142 | 18 | 143 | 2.31e-86 | 248 |
| MS.gene015411.t1 | MTR_4g006830 | 94.444 | 126 | 7 | 0 | 17 | 142 | 19 | 144 | 3.11e-85 | 246 |
| MS.gene015411.t1 | MTR_5g012890 | 96.262 | 107 | 4 | 0 | 1 | 107 | 1 | 107 | 5.85e-71 | 208 |
| MS.gene015411.t1 | MTR_4g073510 | 70.370 | 54 | 14 | 1 | 1 | 52 | 1 | 54 | 6.42e-17 | 76.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015411.t1 | AT2G32060 | 74.400 | 125 | 31 | 1 | 18 | 142 | 21 | 144 | 1.33e-66 | 199 |
| MS.gene015411.t1 | AT2G32060 | 74.400 | 125 | 31 | 1 | 18 | 142 | 21 | 144 | 1.33e-66 | 199 |
| MS.gene015411.t1 | AT2G32060 | 74.400 | 125 | 31 | 1 | 18 | 142 | 21 | 144 | 1.33e-66 | 199 |
| MS.gene015411.t1 | AT1G15930 | 74.400 | 125 | 31 | 1 | 18 | 142 | 21 | 144 | 5.31e-64 | 192 |
| MS.gene015411.t1 | AT1G15930 | 74.400 | 125 | 31 | 1 | 18 | 142 | 21 | 144 | 5.31e-64 | 192 |
Find 33 sgRNAs with CRISPR-Local
Find 91 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTGTTGCTGCTGCAATTCC+AGG | 0.269519 | 5.2:+5077949 | MS.gene015411:CDS |
| TTCACCAATTTGACATAATC+AGG | 0.271702 | 5.2:-5078120 | None:intergenic |
| GATGCTCTTGTGTGGTTGTC+AGG | 0.354575 | 5.2:+5078439 | MS.gene015411:CDS |
| ATGCTGCAAAACAACATTGT+AGG | 0.363171 | 5.2:-5078575 | None:intergenic |
| CATAAGCACGAGACTTTCTA+AGG | 0.363450 | 5.2:-5078008 | None:intergenic |
| CCAACCTGATTATGTCAAAT+TGG | 0.373462 | 5.2:+5078116 | MS.gene015411:CDS |
| CTTAGAAAGTCTCGTGCTTA+TGG | 0.383982 | 5.2:+5078009 | MS.gene015411:CDS |
| ATCAGGTTGGTCACAATCTT+CGG | 0.412966 | 5.2:-5078103 | None:intergenic |
| ATGATGTCCATTGGCTCGCC+TGG | 0.437864 | 5.2:-5077967 | None:intergenic |
| TCTCCAAGGGTCTTTGCACT+TGG | 0.459795 | 5.2:-5078183 | None:intergenic |
| TATCAACCAAGAAATATGTC+AGG | 0.459989 | 5.2:+5077058 | None:intergenic |
| GGAATTGCAGCAGCAACAAC+AGG | 0.463311 | 5.2:-5077946 | None:intergenic |
| GGTAAAGCTAGGAAGGTTAC+CGG | 0.476827 | 5.2:+5078417 | MS.gene015411:CDS |
| GACAACCACACAAGAGCATC+CGG | 0.522597 | 5.2:-5078436 | None:intergenic |
| TTACGCCAGCCCACTCTCCA+AGG | 0.529322 | 5.2:-5078197 | None:intergenic |
| CCAATTTGACATAATCAGGT+TGG | 0.530247 | 5.2:-5078116 | None:intergenic |
| TGCAAAGACCCTTGGAGAGT+GGG | 0.530356 | 5.2:+5078188 | MS.gene015411:CDS |
| TTTGTTATGCAGTGAAGAAG+AGG | 0.537025 | 5.2:+5077909 | MS.gene015411:intron |
| GTTCCAAGTGCAAAGACCCT+TGG | 0.537039 | 5.2:+5078180 | MS.gene015411:CDS |
| AAGACCCTTGGAGAGTGGGC+TGG | 0.538691 | 5.2:+5078192 | MS.gene015411:CDS |
| TTGATTCAGAAGGTAAAGCT+AGG | 0.543764 | 5.2:+5078406 | MS.gene015411:CDS |
| TTCAGAAGGTAAAGCTAGGA+AGG | 0.557714 | 5.2:+5078410 | MS.gene015411:CDS |
| TGCAATTCCAGGCGAGCCAA+TGG | 0.568250 | 5.2:+5077960 | MS.gene015411:CDS |
| GTGCAAAGACCCTTGGAGAG+TGG | 0.569975 | 5.2:+5078187 | MS.gene015411:CDS |
| ATGCTCTTGTGTGGTTGTCA+GGG | 0.586706 | 5.2:+5078440 | MS.gene015411:CDS |
| AAAGCAGTCATGATGTCCAT+TGG | 0.589394 | 5.2:-5077976 | None:intergenic |
| CTTGCACGAGGTCTTCATGA+AGG | 0.605005 | 5.2:+5078036 | MS.gene015411:CDS |
| AGAAAGTCTCGTGCTTATGG+TGG | 0.620799 | 5.2:+5078012 | MS.gene015411:CDS |
| TTGTGCAAGATTGATTCAGA+AGG | 0.623879 | 5.2:+5078396 | MS.gene015411:CDS |
| TACGCCAGCCCACTCTCCAA+GGG | 0.624257 | 5.2:-5078196 | None:intergenic |
| GCTTATGGTGGTCTTGCACG+AGG | 0.624672 | 5.2:+5078024 | MS.gene015411:CDS |
| ATCAACCAAGAAATATGTCA+GGG | 0.636797 | 5.2:+5077059 | None:intergenic |
| GGTTACCGGATGCTCTTGTG+TGG | 0.638821 | 5.2:+5078431 | MS.gene015411:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAGTTCACAATATAAATT+TGG | - | chr5.2:5078286-5078305 | None:intergenic | 15.0% |
| !! | AAAGTTCACAATATAAATTT+GGG | - | chr5.2:5078285-5078304 | None:intergenic | 15.0% |
| !!! | ATTCTTCTATTTATATGTTT+TGG | + | chr5.2:5077809-5077828 | MS.gene015411:intron | 15.0% |
| !! | ACAATATAAATTTGGGAATT+GGG | - | chr5.2:5078278-5078297 | None:intergenic | 20.0% |
| !! | ATTACTTGTAGTTATTACTA+AGG | - | chr5.2:5077697-5077716 | None:intergenic | 20.0% |
| !! | TGCTAATTTATTCTATCATA+TGG | + | chr5.2:5077088-5077107 | MS.gene015411:intron | 20.0% |
| !! | TTCAAATGCAATTGTAATTT+AGG | + | chr5.2:5077125-5077144 | MS.gene015411:intron | 20.0% |
| !!! | TTTATTTTTTTCTTTTGCTG+TGG | - | chr5.2:5077201-5077220 | None:intergenic | 20.0% |
| ! | AAAAAATAAGCCAATCCAAA+CGG | - | chr5.2:5077394-5077413 | None:intergenic | 25.0% |
| ! | AAAAATAAGACAATGCAAAC+GGG | + | chr5.2:5077568-5077587 | MS.gene015411:intron | 25.0% |
| ! | AAAACTAGGTTAAGAATCAA+GGG | - | chr5.2:5078226-5078245 | None:intergenic | 25.0% |
| ! | AACTTATGAAAACAGCTTAT+GGG | - | chr5.2:5077244-5077263 | None:intergenic | 25.0% |
| ! | ATCCTGCAAACAAAAAAAAA+AGG | - | chr5.2:5078536-5078555 | None:intergenic | 25.0% |
| ! | ATCTTAAACGATAAAACTAC+GGG | - | chr5.2:5077611-5077630 | None:intergenic | 25.0% |
| ! | CAAAAATAAGACAATGCAAA+CGG | + | chr5.2:5077567-5077586 | MS.gene015411:intron | 25.0% |
| ! | CACAATATAAATTTGGGAAT+TGG | - | chr5.2:5078279-5078298 | None:intergenic | 25.0% |
| ! | GATCTTAAACGATAAAACTA+CGG | - | chr5.2:5077612-5077631 | None:intergenic | 25.0% |
| ! | TAAAACTAGGTTAAGAATCA+AGG | - | chr5.2:5078227-5078246 | None:intergenic | 25.0% |
| ! | TCCTGCAAACAAAAAAAAAA+GGG | - | chr5.2:5078535-5078554 | None:intergenic | 25.0% |
| ! | TTAGCATGAAAAAAGACAAT+AGG | - | chr5.2:5078494-5078513 | None:intergenic | 25.0% |
| !! | AATGTATAGAACTTTTTACC+AGG | - | chr5.2:5077792-5077811 | None:intergenic | 25.0% |
| !! | TAAAGGTTTTTAAACTTGCA+AGG | - | chr5.2:5077862-5077881 | None:intergenic | 25.0% |
| !!! | TTTTTTGTTTGCAGGATTTT+GGG | + | chr5.2:5078539-5078558 | MS.gene015411:intron | 25.0% |
| !!! | TTTTTTTGTTTGCAGGATTT+TGG | + | chr5.2:5078538-5078557 | MS.gene015411:intron | 25.0% |
| AAAAATAAGCCAATCCAAAC+GGG | - | chr5.2:5077393-5077412 | None:intergenic | 30.0% | |
| AAAATAAGACAATGCAAACG+GGG | + | chr5.2:5077569-5077588 | MS.gene015411:intron | 30.0% | |
| ACAAGGAATTGGATAAAACT+AGG | - | chr5.2:5078240-5078259 | None:intergenic | 30.0% | |
| ACCACTAGATAAGTTCAATT+AGG | + | chr5.2:5077360-5077379 | MS.gene015411:intron | 30.0% | |
| ACTAGTGAGCAACTTATTAA+AGG | - | chr5.2:5077879-5077898 | None:intergenic | 30.0% | |
| AGATAACTGAATCTGATATC+CGG | + | chr5.2:5077668-5077687 | MS.gene015411:intron | 30.0% | |
| CAACTTATGAAAACAGCTTA+TGG | - | chr5.2:5077245-5077264 | None:intergenic | 30.0% | |
| TGGAAATGAGTTATGATAAG+TGG | - | chr5.2:5077181-5077200 | None:intergenic | 30.0% | |
| TTCACCAATTTGACATAATC+AGG | - | chr5.2:5078123-5078142 | None:intergenic | 30.0% | |
| !! | TTTTTGTTTGCAGGATTTTG+GGG | + | chr5.2:5078540-5078559 | MS.gene015411:intron | 30.0% |
| !!! | TCCCTTTTTTTTTTGTTTGC+AGG | + | chr5.2:5078531-5078550 | MS.gene015411:intron | 30.0% |
| AAATAAGCTGAAGACAGCTA+AGG | + | chr5.2:5077282-5077301 | MS.gene015411:intron | 35.0% | |
| AGATAAGTTCAATTAGGCCA+AGG | + | chr5.2:5077366-5077385 | MS.gene015411:intron | 35.0% | |
| ATCAATCTTGCACAACTACA+TGG | - | chr5.2:5078391-5078410 | None:intergenic | 35.0% | |
| ATCAGATGTTTGTCCAAACA+GGG | - | chr5.2:5077151-5077170 | None:intergenic | 35.0% | |
| ATGCTGCAAAACAACATTGT+AGG | - | chr5.2:5078578-5078597 | None:intergenic | 35.0% | |
| ATTGTAATTTAGGCCCTGTT+TGG | + | chr5.2:5077135-5077154 | MS.gene015411:intron | 35.0% | |
| CCAACCTGATTATGTCAAAT+TGG | + | chr5.2:5078116-5078135 | MS.gene015411:CDS | 35.0% | |
| CCAATTTGACATAATCAGGT+TGG | - | chr5.2:5078119-5078138 | None:intergenic | 35.0% | |
| GATAAGTTCAATTAGGCCAA+GGG | + | chr5.2:5077367-5077386 | MS.gene015411:intron | 35.0% | |
| GCCTAATTGAACTTATCTAG+TGG | - | chr5.2:5077364-5077383 | None:intergenic | 35.0% | |
| GTAGTTATTACTAAGGTGAC+CGG | - | chr5.2:5077690-5077709 | None:intergenic | 35.0% | |
| TATCAGATGTTTGTCCAAAC+AGG | - | chr5.2:5077152-5077171 | None:intergenic | 35.0% | |
| TTGATTCAGAAGGTAAAGCT+AGG | + | chr5.2:5078406-5078425 | MS.gene015411:CDS | 35.0% | |
| ! | TTGTGCAAGATTGATTCAGA+AGG | + | chr5.2:5078396-5078415 | MS.gene015411:CDS | 35.0% |
| ! | TTTGTTATGCAGTGAAGAAG+AGG | + | chr5.2:5077909-5077928 | MS.gene015411:intron | 35.0% |
| !! | GCTGTTTTCATAAGTTGTTG+TGG | + | chr5.2:5077248-5077267 | MS.gene015411:intron | 35.0% |
| AAAGCAGTCATGATGTCCAT+TGG | - | chr5.2:5077979-5077998 | None:intergenic | 40.0% | |
| AATGGATGTGCACAAGGAAT+TGG | - | chr5.2:5078251-5078270 | None:intergenic | 40.0% | |
| AGCTATACATGAGACACACA+AGG | - | chr5.2:5077836-5077855 | None:intergenic | 40.0% | |
| ATTTGGGAATTGGGAGCAAA+TGG | - | chr5.2:5078269-5078288 | None:intergenic | 40.0% | |
| CATAAGCACGAGACTTTCTA+AGG | - | chr5.2:5078011-5078030 | None:intergenic | 40.0% | |
| CTTAGAAAGTCTCGTGCTTA+TGG | + | chr5.2:5078009-5078028 | MS.gene015411:CDS | 40.0% | |
| TGTCTCAAGATTTATGCTCC+TGG | + | chr5.2:5077771-5077790 | MS.gene015411:intron | 40.0% | |
| TTCAGAAGGTAAAGCTAGGA+AGG | + | chr5.2:5078410-5078429 | MS.gene015411:CDS | 40.0% | |
| !! | ATCAGGTTGGTCACAATCTT+CGG | - | chr5.2:5078106-5078125 | None:intergenic | 40.0% |
| AGAAAGTCTCGTGCTTATGG+TGG | + | chr5.2:5078012-5078031 | MS.gene015411:CDS | 45.0% | |
| ATGCTCTTGTGTGGTTGTCA+GGG | + | chr5.2:5078440-5078459 | MS.gene015411:CDS | 45.0% | |
| GATAAAACTACGGGCCTAGT+TGG | - | chr5.2:5077602-5077621 | None:intergenic | 45.0% | |
| GGTAAAGCTAGGAAGGTTAC+CGG | + | chr5.2:5078417-5078436 | MS.gene015411:CDS | 45.0% | |
| !! | TTGTTTGCAGGATTTTGGGG+AGG | + | chr5.2:5078543-5078562 | MS.gene015411:intron | 45.0% |
| !!! | TATTTATTTTATTGATTTTT+GGG | + | chr5.2:5078356-5078375 | MS.gene015411:intron | 5.0% |
| !!! | TTATTTATTTTATTGATTTT+TGG | + | chr5.2:5078355-5078374 | MS.gene015411:intron | 5.0% |
| AACGGGGCCTAAATCCAACT+AGG | + | chr5.2:5077585-5077604 | MS.gene015411:intron | 50.0% | |
| CTACGGGCCTAGTTGGATTT+AGG | - | chr5.2:5077595-5077614 | None:intergenic | 50.0% | |
| CTTGCACGAGGTCTTCATGA+AGG | + | chr5.2:5078036-5078055 | MS.gene015411:CDS | 50.0% | |
| CTTGTGTGGTTGTCAGGGTA+TGG | + | chr5.2:5078445-5078464 | MS.gene015411:intron | 50.0% | |
| GATGCTCTTGTGTGGTTGTC+AGG | + | chr5.2:5078439-5078458 | MS.gene015411:CDS | 50.0% | |
| GGAATTGCAGCAGCAACAAC+AGG | - | chr5.2:5077949-5077968 | None:intergenic | 50.0% | |
| GGAGCAAATGGATGTGCACA+AGG | - | chr5.2:5078257-5078276 | None:intergenic | 50.0% | |
| GTTCCAAGTGCAAAGACCCT+TGG | + | chr5.2:5078180-5078199 | MS.gene015411:CDS | 50.0% | |
| GTTGTTGCTGCTGCAATTCC+AGG | + | chr5.2:5077949-5077968 | MS.gene015411:CDS | 50.0% | |
| TCTCCAAGGGTCTTTGCACT+TGG | - | chr5.2:5078186-5078205 | None:intergenic | 50.0% | |
| TGCAAAGACCCTTGGAGAGT+GGG | + | chr5.2:5078188-5078207 | MS.gene015411:CDS | 50.0% | |
| !! | GACAACCACACAAGAGCATC+CGG | - | chr5.2:5078439-5078458 | None:intergenic | 50.0% |
| AATTAGGCCAAGGGCCCGTT+TGG | + | chr5.2:5077376-5077395 | MS.gene015411:intron | 55.0% | |
| GGTTACCGGATGCTCTTGTG+TGG | + | chr5.2:5078431-5078450 | MS.gene015411:CDS | 55.0% | |
| GTGCAAAGACCCTTGGAGAG+TGG | + | chr5.2:5078187-5078206 | MS.gene015411:CDS | 55.0% | |
| TGCAATTCCAGGCGAGCCAA+TGG | + | chr5.2:5077960-5077979 | MS.gene015411:CDS | 55.0% | |
| ! | GCTTATGGTGGTCTTGCACG+AGG | + | chr5.2:5078024-5078043 | MS.gene015411:CDS | 55.0% |
| !! | ATGATGTCCATTGGCTCGCC+TGG | - | chr5.2:5077970-5077989 | None:intergenic | 55.0% |
| AAGACCCTTGGAGAGTGGGC+TGG | + | chr5.2:5078192-5078211 | MS.gene015411:CDS | 60.0% | |
| AGCCAATCCAAACGGGCCCT+TGG | - | chr5.2:5077386-5077405 | None:intergenic | 60.0% | |
| GCAACAAGACTGCGCGAGCA+TGG | - | chr5.2:5078329-5078348 | None:intergenic | 60.0% | |
| TACGCCAGCCCACTCTCCAA+GGG | - | chr5.2:5078199-5078218 | None:intergenic | 60.0% | |
| TTACGCCAGCCCACTCTCCA+AGG | - | chr5.2:5078200-5078219 | None:intergenic | 60.0% | |
| GGCCAAGGGCCCGTTTGGAT+TGG | + | chr5.2:5077381-5077400 | MS.gene015411:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 5077073 | 5078612 | 5077073 | ID=MS.gene015411 |
| chr5.2 | mRNA | 5077073 | 5078612 | 5077073 | ID=MS.gene015411.t1;Parent=MS.gene015411 |
| chr5.2 | exon | 5077073 | 5077080 | 5077073 | ID=MS.gene015411.t1.exon1;Parent=MS.gene015411.t1 |
| chr5.2 | CDS | 5077073 | 5077080 | 5077073 | ID=cds.MS.gene015411.t1;Parent=MS.gene015411.t1 |
| chr5.2 | exon | 5077921 | 5078215 | 5077921 | ID=MS.gene015411.t1.exon2;Parent=MS.gene015411.t1 |
| chr5.2 | CDS | 5077921 | 5078215 | 5077921 | ID=cds.MS.gene015411.t1;Parent=MS.gene015411.t1 |
| chr5.2 | exon | 5078396 | 5078461 | 5078396 | ID=MS.gene015411.t1.exon3;Parent=MS.gene015411.t1 |
| chr5.2 | CDS | 5078396 | 5078461 | 5078396 | ID=cds.MS.gene015411.t1;Parent=MS.gene015411.t1 |
| chr5.2 | exon | 5078553 | 5078612 | 5078553 | ID=MS.gene015411.t1.exon4;Parent=MS.gene015411.t1 |
| chr5.2 | CDS | 5078553 | 5078612 | 5078553 | ID=cds.MS.gene015411.t1;Parent=MS.gene015411.t1 |
| Gene Sequence |
| Protein sequence |
